keyword
MENU ▼
Read by QxMD icon Read
search

Pyruvate dehydrogenase

keyword
https://www.readbyqxmd.com/read/28814762/down-regulation-of-pdk4-is-critical-for-the-switch-of-carbohydrate-catabolism-during-syncytialization-of-human-placental-trophoblasts
#1
Xiaohui Liu, Rujuan Zuo, Yirong Bao, Xiaoxian Qu, Kang Sun, Hao Ying
Pyruvate dehydrogenase kinase (PDK) is known as a gatekeeper directing the carbon flux into glycolysis via inhibition of the pyruvate dehydrogenase complex. During syncytialization of placental trophoblasts, both ATP production and oxygen consumption are increased to meet enhanced energetic demands by syntiotrophoblasts. We hypothesized that down-regulation of PDK expression may play a central role in the switch from glycolysis to oxidative phosphorylation (OXPHOS) during syncytialization. By using primary human trophoblasts, we demonstrated that PDK4 was the dominating PDK isoform in human cytotrophoblasts, and its abundance was substantially decreased upon syncytialization, which was accompanied by decreases in lactate production and increases in ATP production...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812580/lactate-dehydrogenase-activity-drives-hair-follicle-stem-cell-activation
#2
Aimee Flores, John Schell, Abigail S Krall, David Jelinek, Matilde Miranda, Melina Grigorian, Daniel Braas, Andrew C White, Jessica L Zhou, Nicholas A Graham, Thomas Graeber, Pankaj Seth, Denis Evseenko, Hilary A Coller, Jared Rutter, Heather R Christofk, William E Lowry
Although normally dormant, hair follicle stem cells (HFSCs) quickly become activated to divide during a new hair cycle. The quiescence of HFSCs is known to be regulated by a number of intrinsic and extrinsic mechanisms. Here we provide several lines of evidence to demonstrate that HFSCs utilize glycolytic metabolism and produce significantly more lactate than other cells in the epidermis. Furthermore, lactate generation appears to be critical for the activation of HFSCs as deletion of lactate dehydrogenase (Ldha) prevented their activation...
August 14, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28812382/can-elevated-lactate-and-ldh-produce-a-false-positive-enzymatic-ethanol-result-in-live-patients-presenting-to-the-emergency-department
#3
Nicholas Nacca, Michael J Hodgman, Kirselle Lao, Matthew Elkins, Michael G Holland
BACKGROUND: There have been allegations in the courtroom that elevated serum lactic acid in trauma victims can yield a falsely elevated serum ethanol assay. Most hospitals utilize an indirect method of ethanol measurement where a serum sample is added to a mix of alcohol dehydrogenase and oxidized nicotinamide adenine dinucleotide (NAD+). This allows any ethanol in the patient's serum to be metabolized to acetaldehyde, and in the process results in the reduction of NAD + to NADH. NADH is then measured using spectrophotometry...
August 16, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28808393/evaluation-of-the-antidiabetic-properties-of-s-1708-mulberry-variety
#4
Brijesh Ranjan, Randhir Kumar, Neeraj Verma, Swati Mittal, Pranab Lal Pakrasi, R Venkatesh Kumar
BACKGROUND: Diabetes is a metabolic disease prevalent worldwide in all age group of people. The source of diabetes is due to an oxidation process that can produce free radicals. An increase in oxidative free radicals in the body is reported to be one of the several causes of diabetes. The best remedy to combat oxidative stress is the use of antioxidants, which inhibit and scavenge free radicals. AIM: This study has been undertaken to evaluate the antioxidant activity and antidiabetic effect of mulberry leaf extract in diabetic mice...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28807341/ultrastructural-examination-of-skin-biopsies-may-assist-in-diagnosing-mitochondrial-cytopathy-when-muscle-biopsies-yield-negative-results
#5
John L McAfee, Christine B Warren, Richard A Prayson
Ultrastructural evaluation of skin biopsies has been utilized for diagnosis of mitochondrial disease. This study investigates how frequently skin biopsies reveal mitochondrial abnormalities, correlates skin and muscle biopsy findings, and describes clinical diagnoses rendered following the evaluation. A retrospective review of surgical pathology reports from 1990 to 2015 identified skin biopsies examined by electron microscopy for suspected metabolic disease. A total of 630 biopsies were included from 615 patients...
August 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/28802880/isobutanol-and-2-ketoisovalerate-production-by-klebsiella-pneumoniae-via-a-native-pathway
#6
Jinjie Gu, Jidong Zhou, Zhongxi Zhang, Chul Ho Kim, Biao Jiang, Jiping Shi, Jian Hao
Isobutanol is a valuable chemical and is considered a new generation biofuel. Construction of isobutanol synthesis pathways in bacteria is a hot topic in isobutanol production. Here, we show that an isobutanol synthesis pathway exists naturally in Klebsiella pneumoniae; however, this pathway is dormant in the wild-type bacterium. K. pneumoniae is a 2,3-butanediol producer, and the synthesis pathways of 2,3-butanediol, valine and isobutanol all start from condensation of two pyruvate molecules to yield α-acetolactate...
August 9, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28801776/effects-of-training-status-on-pdh-regulation-in-human-skeletal-muscle-during-exercise
#7
Anders Gudiksen, Lærke Bertholdt, Tomasz Stankiewicz, Jonas Tybirk, Peter Plomgaard, Jens Bangsbo, Henriette Pilegaard
Pyruvate dehydrogenase (PDH) is the gateway enzyme for carbohydrate-derived pyruvate feeding into the TCA cycle. PDH may play a central role in regulating substrate shifts during exercise, but the influence of training state on PDH regulation during exercise is not fully elucidated. The purpose of this study was to investigate the impact of training state on post-translational regulation of PDHa activity during submaximal and exhaustive exercise. Eight untrained and nine endurance exercise-trained healthy male subjects performed incremental exercise on a cycle ergometer: 40 min at 50% incremental peak power output (IPPO), 10 min at 65% (IPPO), followed by 80% (IPPO) until exhaustion...
August 11, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28801642/extreme-anoxia-tolerance-in-crucian-carp-and-goldfish-through-neofunctionalization-of-duplicated-genes-creating-a-new-ethanol-producing-pyruvate-decarboxylase-pathway
#8
Cathrine E Fagernes, Kåre-Olav Stensløkken, Åsmund K Røhr, Michael Berenbrink, Stian Ellefsen, Göran E Nilsson
Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to survive prolonged periods without oxygen by producing ethanol as their metabolic end-product. Here we show that this has been made possible by the evolution of a pyruvate decarboxylase, analogous to that in brewer's yeast and the first described in vertebrates, in addition to a specialized alcohol dehydrogenase...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28799513/ketogenic-diets-in-the-treatment-of-epilepsy
#9
Maurizio Elia, Joerg Klepper, Baerbel Leiendecker, Hans Hartmann
BACKGROUND: Although a larger number of antiepileptic drugs became available in the last decades, epilepsy remains drug-resistant in approximately a third of patients. Ketogenic diet (KD), first proposed at the beginning of the last century, is complex and has anticonvulsant effects, yet not completely understood. Over the last decades, different types of ketogenic diets (KDs) have been developed, namely classical KD and modified Atkins diet (MAD). They offer an effective alternative for children and adults with drug-resistant epilepsies...
August 9, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28795319/levofloxacin-is-phytotoxic-and-modifies-the-protein-profile-of-lupin-seedlings
#10
Aleksandra Orzoł, Agnieszka I Piotrowicz-Cieślak
The toxicity of levofloxacin to yellow lupin plants was evaluated in this study. Recommended indexes of plant (roots and shoots) growth were determined and new indexes were proposed which better characterise the phytotoxicity of levofloxacin. These were, in particular, the activity of antioxidative enzymes, the content of free radicals, as well as the root protein content and the root protein profile. The results showed that levofloxacin considerably affected EC50, measured as the activity of catalase in roots, and leaves (1...
August 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28781146/the-impact-of-foxo-1-to-cardiac-pathology-in-diabetes-mellitus-and-diabetes-related-metabolic-abnormalities
#11
Dimitry A Chistiakov, Alexander N Orekhov, Yuri V Bobryshev
Diabetic heart pathology has a serious social impact due to high prevalence worldwide and significant mortality/invalidation of diabetic patients suffered from cardiomyopathy. The pathogenesis of diabetic and diabetes-related cardiomyopathy is associated with progressive loss and impairment of cardiac function due to adverse effects of metabolic, prooxidant, proinflammatory, and pro-apoptotic stress factors. In the adult heart, the transcriptional factor forkhead box-1 (FOXO-1) is involved in maintaining cardiomyocytes in the homeostatic state and induction of their adaptation to metabolic and pro-oxidant stress stimuli...
August 2, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28771799/modulation-of-nfkb1-p50-by-ros-leads-to-impaired-atp-production-during-mi-compared-to-cardiac-hypertrophy
#12
Arkadeep Mitra, Ritwik Datta, Santanu Rana, Sagartirtha Sarkar
Pathological hypertrophy and myocardial infarction (MI) are two etiologically different cardiac disorders having differential molecular mechanisms of disease manifestation. However, no study has been conducted so far to analyze and compare the differential status of energy metabolism in these two disease forms. It was shown recently by our group that production of ATP is significantly impaired during MI along with inhibition of pyruvate dehydrogenase E1-β (PDHE1 B) by pyruvate dehydrogenase kinase 4 (PDK4)...
August 3, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28768647/activation-of-transient-receptor-potential-melastatin-subtype-8-attenuates-cold-induced-hypertension-through-ameliorating-vascular-mitochondrial-dysfunction
#13
Shiqiang Xiong, Bin Wang, Shaoyang Lin, Hexuan Zhang, Yingsha Li, Xing Wei, Yuanting Cui, Xiao Wei, Zongshi Lu, Peng Gao, Li Li, Zhigang Zhao, Daoyan Liu, Zhiming Zhu
BACKGROUND: Environmental cold-induced hypertension is common, but how to treat cold-induced hypertension remains an obstacle. Transient receptor potential melastatin subtype 8 (TRPM8) is a mild cold-sensing nonselective cation channel that is activated by menthol. Little is known about the effect of TRPM8 activation by menthol on mitochondrial Ca(2+) homeostasis and the vascular function in cold-induced hypertension. METHODS AND RESULTS: Primary vascular smooth muscle cells from wild-type or Trpm8(-/-) mice were cultured...
August 2, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28767746/exogenous-catalase-and-pyruvate-dehydrogenase-improve-survival-and-regeneration-and-affect-oxidative-stress-in-cryopreserved-dendrobium-nobile-protocorm-like-bodies
#14
W Di, M X Jia, J Xu, B L Li, Y Liu
BACKGROUND: Reactive oxygen species (ROS)-induced oxidative damage is responsible for viability loss in plant tissues following cryopreservation. Antioxidants may improve viability by preventing or repairing the injury. OBJECTIVE: This work aimed at studying the effect of catalase (CAT) and pyruvate dehydrogenase (PDH), which are involved in ROS metabolism and are differentially expressed during pollen cryopreservation, for cryopreservation of Dendrobium nobile Lindl...
May 2017: Cryo Letters
https://www.readbyqxmd.com/read/28763106/identification-of-proteome-antigen-protein-and-antigen-membrane-protein-from-spiroplasma-eriocheiris
#15
Yuhan Liu, Yinbin Xu, Shuang Li, Xuechuan Xu, Qi Gao, Meijun Yuan, Wei Gu, Wen Wang, Qingguo Meng
Spiroplasma eriocheiris, which causes tremor disease of Chinese mitten crab Eriocheir sinensis, has led to catastrophic economic loses in aquaculture. Immunoproteomics, a new scientific technique combining proteomics and immunological analytical methods, provided the direction of our research on S. eriocheiris. The aim of our study was to identify the proteome, antigen proteins and antigen membrane proteins of S. eriocheiris. A total of 780 S. eriocheiris proteins were identified by LC-MS/MS technique. Based on immunoproteomics, 51 proteins and 7 proteins in S...
August 1, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28761044/resveratrol-reverses-the-warburg-effect-by-targeting-the-pyruvate-dehydrogenase-complex-in-colon-cancer-cells
#16
Elise Saunier, Samantha Antonio, Anne Regazzetti, Nicolas Auzeil, Olivier Laprévote, Jerry W Shay, Xavier Coumoul, Robert Barouki, Chantal Benelli, Laurence Huc, Sylvie Bortoli
Resveratrol (RES), a polyphenol found in natural foods, displays anti-oxidant, anti-inflammatory and anti-proliferative properties potentially beneficial in cancers, in particular in the prevention of tumor growth. However, the rapid metabolism of resveratrol strongly limits its bioavailability. The molecular mechanisms sustaining the potential biological activity of low doses of resveratrol has not been extensively studied and, thus, needs better characterization. Here, we show that resveratrol (10 µM, 48 hr) induces both a cell growth arrest and a metabolic reprogramming in colon cancer cells...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28760957/hyperpolarized-13-c-mr-metabolic-imaging-can-detect-neuroinflammation-in-vivo-in-a-multiple-sclerosis-murine-model
#17
Caroline Guglielmetti, Chloé Najac, Alessandro Didonna, Annemie Van der Linden, Sabrina M Ronen, Myriam M Chaumeil
Proinflammatory mononuclear phagocytes (MPs) play a crucial role in the progression of multiple sclerosis (MS) and other neurodegenerative diseases. Despite advances in neuroimaging, there are currently limited available methods enabling noninvasive detection of MPs in vivo. Interestingly, upon activation and subsequent differentiation toward a proinflammatory phenotype MPs undergo metabolic reprogramming that results in increased glycolysis and production of lactate. Hyperpolarized (HP) (13)C magnetic resonance spectroscopic imaging (MRSI) is a clinically translatable imaging method that allows noninvasive monitoring of metabolic pathways in real time...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28759252/long-noncoding-rna-cancer-susceptibility-candidate-8-suppresses-the-proliferation-of-bladder-cancer-cells-via-regulating-glycolysis
#18
Renguang Hu, Peng Zhong, Lu Xiong, Liangbin Duan
Increasing evidence highlights the critical regulatory role of long noncoding RNAs (lncRNAs) in carcinogenesis. Bladder cancer has become the most prevalent urologic malignancy, which is commonly diagnosed among adults. In this study, we showed that the lncRNA cancer susceptibility candidate 8 (CASC8) is significantly downregulated in bladder cancers and associated with the advanced stage of bladder cancer patients. Overexpression of CASC8 remarkably suppressed the bladder cancer cell proliferation. Mechanistically, we illustrated that CASC8 reduced the glycolysis of bladder cancer cells via interacting with the fibroblast growth factor receptor 1 (FGFR1)...
July 31, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28757214/vulnerability-of-marsh-frog-pelophylax-ridibundus-to-the-typical-wastewater-effluents-ibuprofen-triclosan-and-estrone-detected-by-multi-biomarker-approach
#19
Halina I Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, Oksana B Stoliar
Pharmaceutical and personal care products (PPCPs) are the environmental pollutants of growing concern. The aim of this study was to indicate the effects of typical PPCPs on the marsh frog Pelophylax ridibundus. We treated male frogs with waterborne ibuprofen (IBU, 250ng·L(-1)), triclosan (TCS, 500ng·L(-1)), or estrone (E1, 100ng·L(-1)) for 14days. Common vulnerability of the frogs was detected from dramatic decrease of Zn, total and metalated metallothionein (MT) concentrations, Zn/Cu ratio, the elevation of activity of glutathione-S-transferase, cathepsin D and DNA instability in the liver, the depletion of cholinesterase in the brain and cortisol in the blood plasma in all exposures...
July 27, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28757203/biallelic-mutations-in-lipt2-cause-a-mitochondrial-lipoylation-defect-associated-with-severe-neonatal-encephalopathy
#20
Florence Habarou, Yamina Hamel, Tobias B Haack, René G Feichtinger, Elise Lebigot, Iris Marquardt, Kanetee Busiah, Cécile Laroche, Marine Madrange, Coraline Grisel, Clément Pontoizeau, Monika Eisermann, Audrey Boutron, Dominique Chrétien, Bernadette Chadefaux-Vekemans, Robert Barouki, Christine Bole-Feysot, Patrick Nitschke, Nicolas Goudin, Nathalie Boddaert, Ivan Nemazanyy, Agnès Delahodde, Stefan Kölker, Richard J Rodenburg, G Christoph Korenke, Thomas Meitinger, Tim M Strom, Holger Prokisch, Agnes Rotig, Chris Ottolenghi, Johannes A Mayr, Pascale de Lonlay
Lipoate serves as a cofactor for the glycine cleavage system (GCS) and four 2-oxoacid dehydrogenases functioning in energy metabolism (α-oxoglutarate dehydrogenase [α-KGDHc] and pyruvate dehydrogenase [PDHc]), or amino acid metabolism (branched-chain oxoacid dehydrogenase, 2-oxoadipate dehydrogenase). Mitochondrial lipoate synthesis involves three enzymatic steps catalyzed sequentially by lipoyl(octanoyl) transferase 2 (LIPT2), lipoic acid synthetase (LIAS), and lipoyltransferase 1 (LIPT1). Mutations in LIAS have been associated with nonketotic hyperglycinemia-like early-onset convulsions and encephalopathy combined with a defect in mitochondrial energy metabolism...
August 3, 2017: American Journal of Human Genetics
keyword
keyword
94832
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"