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https://www.readbyqxmd.com/read/29454277/nitrogen-removal-enhancement-using-lactic-acid-fermentation-products-from-food-waste-as-external-carbon-sources-performance-and-microbial-communities
#1
Jialing Tang, Xiaochang C Wang, Yisong Hu, Yunhui Pu, Jin Huang, Huu Hao Ngo, Yonggang Zeng, Yuyou Li
In this study, nitrogen removal using the lactic acid fermentation products from food waste and other external chemical carbon sources (sodium acetate, sodium lactate and starch) was investigated. Similar to sodium acetate and lactate, the lactic acid-enriched fermentation liquid from food waste (FLFW) exhibited a high denitrification rate (5.5 mg NO x -N/(g-VSS h)) and potential (0.16 g NO 3 - -N/g COD), and could achieve high NH 4 + -N and total nitrogen (TN) removal efficiencies during long-term operation...
February 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29454255/effects-of-adjunctive-eslicarbazepine-acetate-on-neurocognitive-functioning-in-children-with-refractory-focal-onset-seizures
#2
Sergiusz Jóźwiak, P Veggiotti, J Moreira, H Gama, F Rocha, P Soares-da-Silva
PURPOSE: This was a phase-II, randomized, double-blind (DB), placebo-controlled study aimed to evaluate neurocognitive effects of eslicarbazepine acetate (ESL) as adjunctive therapy in pediatric patients with refractory focal-onset seizures (FOS). METHODS: Children (6-16years old) with FOS were randomized (2:1) to ESL or placebo. Treatment started at 10mg/kg/day, was up-titrated up to 30mg/kg/day (target dose), and maintained for 8weeks, followed by one-year open-label follow-up...
February 13, 2018: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/29454193/revealing-metabolic-storage-processes-in-electrode-respiring-bacteria-by-differential-electrochemical-mass-spectrometry
#3
F Kubannek, U Schröder, U Krewer
In this work we employ differential electrochemical mass spectrometry (DEMS) in combination with static and dynamic electrochemical techniques for the study of metabolic processes of electrochemically active bacteria. CO 2 production during acetate oxidation by electrode respiring bacteria was measured, in-vivo and online with a sensitivity of 6.5 ⋅ 10 -13 mol/s. The correlation of ion current and electrical current provides insight into the interaction of metabolic processes and extra-cellular electron transfer...
February 8, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29454169/farmyard-manure-alone-and-combined-with-immobilizing-amendments-reduced-cadmium-accumulation-in-wheat-and-rice-grains-grown-in-field-irrigated-with-raw-effluents
#4
Muhammad Zia Ur Rehman, Muhammad Rizwan, Hinnan Khalid, Shafaqat Ali, Asif Naeem, Balal Yousaf, Guijian Liu, Muhammad Sabir, Muhammad Farooq
Cadmium (Cd) stress is a serious concern in agricultural soils worldwide whereas little is known about the impact of farmyard manure (FYM) alone or combined with limestone, lignite and biochar on Cd concentrations in plants. Wheat was grown in Cd-contaminated field amended with control (T 1 ), FYM @ 0.1% (T 2 ), FYM + limestone @ 0.05% each (T 3 ), FYM + lignite @ 0.05% each (T 4 ), FYM + biochar @ 0.05% each (T 5 ) and subsequent rice was grown without additional use of amendments. Soil application of amendments increased straw and grain yield and thousand grain weight being maximum in FYM + limestone treatment...
February 8, 2018: Chemosphere
https://www.readbyqxmd.com/read/29454079/a-novel-metric-that-quantifies-risk-stratification-for-evaluating-diagnostic-tests-the-example-of-evaluating-cervical-cancer-screening-tests-across-populations
#5
Hormuzd A Katki, Mark Schiffman
Our work involves assessing whether new biomarkers might be useful for cervical-cancer screening across populations with different disease prevalences and biomarker distributions. When comparing across populations, we show that standard diagnostic accuracy statistics (predictive values, risk-differences, Youden's index and Area Under the Curve (AUC)) can easily be misinterpreted. We introduce an intuitively simple statistic for a 2 × 2 table, Mean Risk Stratification (MRS): the average change in risk (pre-test vs...
February 14, 2018: Preventive Medicine
https://www.readbyqxmd.com/read/29454033/cause-or-coincidence-spontaneous-hematometra-in-young-women-on-depomedroxyprogesterone-acetate-a-small-case-series
#6
Julie G Thorne, Elizabeth H Russell, Danielle Rumbolt, Mary Anne Jamieson
BACKGROUND: Abdominal pain, secondary amenorrhea and abnormal uterine bleeding (AUB) are common gynecologic presentations in adolescence. Rarely this can be associated with an acquired hematometra. Hematometra is a condition of retained blood or clot within the uterus. High dose progestogenic agents in this age group have been implicated in the accumulation of a hematometra without other explanation. CASES: We present 4 cases of hematometra following depomedroxyprogesterone acetate (DMPA) therapy in previously menstruating adolescents...
February 14, 2018: Journal of Pediatric and Adolescent Gynecology
https://www.readbyqxmd.com/read/29454028/fabricating-3d-printed-orally-disintegrating-printlets-using-selective-laser-sintering
#7
Fabrizio Fina, Christine M Madla, Alvaro Goyanes, Jiaxin Zhang, Simon Gaisford, Abdul W Basit
Selective laser sintering (SLS) is a three-dimensional printing (3DP) technology employed to manufacture plastic, metallic or ceramic objects. The aim of this study was to demonstrate the feasibility of SLS to fabricating novel solid dosage forms with accelerated drug release properties, and with a view to create orally disintegrating formulations. Two polymers (hydroxypropyl methylcellulose (HPMC E5) and vinylpyrrolidone-vinyl acetate copolymer (Kollidon ® VA 64)) were separately mixed with 5% paracetamol (used as a model drug) and 3% Candurin ® gold sheen colorant; the powder mixes were subjected to SLS printing resulting in the manufacture of printlets (3DP tablets)...
February 14, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29453917/comparative-chemical-investigation-and-evaluation-of-antioxidant-and-tyrosinase-inhibitory-effects-of-withania-somnifera-l-dunal-and-solanum-nigrum-l-berries
#8
Abdul Hameed, Naveed Akhtar
In the present study, berries of two different species of Solanaceae family, Withania somnifera (WS) and Solanum nigrum (SN), were extracted in methanol and then fractionated with solvents, ranging from non-polar to polar, for their phytochemical profiling and investigation of antioxidant and tyrosinase enzyme inhibition capacity. The methanolic extract and n-hexane, ethyl acetate (WSEA, SNEA) and aqueous fractions were chemically analyzed and evaluated for biological activity. Total flavonoids and total phenolics were quantified in WSEA (96...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29453912/hptlc-determination-of-diosgenin-in-fenugreek-seeds
#9
Barbara Król-Kogus, Khenifi Mohammed Lamine, Piotr Migas, Messaoud Boudjeniba, Mirosława Krauze-Baranowska
A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29453736/reproductive-epidemiology-of-glial-tumors-may-reveal-novel-treatments-high-dose-progestins-or-progesterone-antagonists-as-endocrino-immune-modifiers-against-glioma
#10
REVIEW
Meric A Altinoz, Aysel Ozpinar, Ilhan Elmaci
Female gender, contraceptives, and menopausal hormone replacement treatments containing progesterone analogues associate with higher risk of meningiomas yet with lower risk of gliomas. Progesterone receptor (PR) expression and mifepristone treatment was highly discussed for meningiomas. However, much less is known in regard to progesterone actions in gliomas despite PR expression strongly correlates with their grade. Meningiomas and gliomas may grow faster during gestation; but paradoxically, parousity reduces lifetime risk of gliomas which can be explained with dichotomous cell growth-stimulating and inhibitory actions of progesterone at low versus high levels...
February 17, 2018: Neurosurgical Review
https://www.readbyqxmd.com/read/29453625/effects-of-feedstock-and-co-culture-of-lactobacillus-fermentum-and-wild-saccharomyces-cerevisiae-strain-during-fuel-ethanol-fermentation-by-the-industrial-yeast-strain-pe-2
#11
Vanda R Reis, Ana Paula G Bassi, Bianca C Cerri, Amanda R Almeida, Isis G B Carvalho, Reinaldo G Bastos, Sandra R Ceccato-Antonini
Even though contamination by bacteria and wild yeasts are frequently observed during fuel ethanol fermentation, our knowledge regarding the effects of both contaminants together is very limited, especially considering that the must composition can vary from exclusively sugarcane juice to a mixture of molasses and juice, affecting the microbial development. Here we studied the effects of the feedstock (sugarcane juice and molasses) and the co-culture of Lactobacillus fermentum and a wild Saccharomyces cerevisiae strain (rough colony and pseudohyphae) in single and multiple-batch fermentation trials with an industrial strain of S...
February 16, 2018: AMB Express
https://www.readbyqxmd.com/read/29453603/repeatable-and-reproducible-measurements-of-myocardial-oxidative-metabolism-blood-flow-and-external-efficiency-using-11-c-acetate-pet
#12
Kai Yi Wu, Vincent Dinculescu, Jennifer M Renaud, Shin-Yee Chen, Ian G Burwash, Lisa M Mielniczuk, Rob S B Beanlands, Robert A deKemp
BACKGROUND: Non-invasive approaches to investigate myocardial efficiency can help track the progression of heart failure (HF). This study evaluates the repeatability and reproducibility of 11 C-acetate positron emission tomography (PET) imaging of oxidative metabolism. METHODS AND RESULTS: Dynamic 11 C-acetate PET scans were performed at baseline and followup (47 ± 22 days apart) in 20 patients with stable HF with reduced ejection fraction. Two observers blinded to patients' clinical data used FlowQuant ® to evaluate test-retest repeatability, as well as intra- and inter-observer reproducibility of 11 C-acetate tracer uptake and clearance rates, for the measurement of myocardial oxygen consumption (MVO 2 ), myocardial external efficiency (MEE), work metabolic index (WMI), and myocardial blood flow...
February 16, 2018: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/29453373/dormancy-release-and-germination-of-taxus-yunnanensis-seeds-during-wet-sand-storage
#13
Fangyuan Bian, Jianrong Su, Wande Liu, Shuaifeng Li
Dormancy is an innate constraint on germination that occurs across all life forms. In this study, we investigated the seed dormancy release and germination characters of Taxus yunnanensis by exploring the seed morphology, permeability, germination inhibitors, endogenous hormones, and embryo germination in vitro during wet sand storage. Our results showed that seeds and embryos grew to a critical size to germination and permeability increased with the extension of storage. Seed coat and kernel methanol extracts reduced Brassica campestris seed vigor index...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453063/-1-h-nmr-based-metabolomics-reveals-interactive-effects-between-the-carrier-solvent-methanol-and-a-pharmaceutical-mixture-in-an-amphibian-developmental-bioassay-with-limnodynastes-peronii
#14
Steven D Melvin, Oliver A H Jones, Anthony R Carroll, Frederic D L Leusch
Organic carrier solvents are used in aquatic toxicity testing to improve chemical solubility and facilitate the exploration of dose-response relationships. Both water- and solvent-control groups are normally included in these scenarios to ensure that the solvent itself has no effect on the test organism, but this fails to consider possible interactive effects between carrier solvents and contaminants of interest. We explored this topic by exposing Limnodynastes peronii tadpoles to a mixture of common water-soluble pharmaceuticals (diclofenac, metformin and valproic acid) in the presence and absence of the carrier solvent methanol, according to standard developmental bioassay methodology...
February 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29453055/enhanced-electrosynthesis-performance-of-moorella-thermoautotrophica-by-improving-cell-permeability
#15
Shanshan Chen, Yanlun Fang, Xianyue Jing, Hailin Luo, Jing Chen, Shungui Zhou
Microbial electrosynthesis systems (MES) are promising devices in which microbes obtain electrons from electrodes to produce extracellular multicarbon compounds. This study investigates whether improvement in cell permeability can enhance electrosynthesis performance of Gram-positive Moorella thermoautotrophica in MES. Results showed that when ≤30mg/L penicillin was added, the cell permeability was doubled, and the electron uptake per biomass (including both cathode-associated biomass and suspended biomass) was 1...
February 8, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29452584/high-phosphorus-intake-and-gut-related-parameters-results-of-a-randomized-placebo-controlled-human-intervention-study
#16
Ulrike Trautvetter, Amélia Camarinha-Silva, Gerhard Jahreis, Stefan Lorkowski, Michael Glei
BACKGROUND: In recent years, high phosphate intakes were discussed critically. In the small intestine, a part of the ingested phosphate and calcium precipitates to amorphous calcium phosphate (ACP), which in turn can precipitate other intestinal substances, thus leading to a beneficial modulation of the intestinal environment. Therefore, we analysed faecal samples obtained from a human intervention study regarding gut-related parameters. METHODS: Sixty-two healthy subjects (men, n = 30; women, n = 32) completed the double-blind, placebo-controlled and parallel designed study (mean age: 29 ± 7 years; mean BMI: 24 ± 3 kg/m 2 )...
February 16, 2018: Nutrition Journal
https://www.readbyqxmd.com/read/29452421/the-short-cosyntropintest-revisited-new-normal-reference-range-using-lcmsms
#17
Grethe Å Ueland, Paal Methlie, Marianne Øksnes, Hrafnkell B Thordarson, Jørn Sagen, Ralf Kellmann, Gunnar Mellgren, Maria Ræder, Per Dahlqvist, Sandra R Dahl, Per M Thorsby, Kristian Løvås, Eystein S Husebye
Background: The cosyntropin test is used to diagnose adrenal insufficiency (AI) and non-classical congenital adrenal hyperplasia (NCCAH). Current cutoffs for cortisol and 17-hydroxyprogesterone (17-OHP) are derived from non-standardized immunoassays. Liquid chromatography tandem mass spectrometry (LCMSMS) offers direct measurement of steroids, prompting the need to re-establish normal ranges. Aim: Define cutoff values for cortisol and 17-OHP in serum by LCMSMS 30 and 60 minutes after intravenous administration of 250 µg tetracosactide acetate (Synachten©) to healthy volunteers, and compare LCMSMS with routine immunoassays...
February 14, 2018: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29452158/protein-kinase-c-epsilon-mediates-the-inhibition-of-angiotensin-ii-on-the-slowly-activating-delayed-rectifier-potassium-current-through-channel-phosphorylation
#18
Xiangbo Gou, Wenying Wang, Sihao Zou, Yajuan Qi, Yanfang Xu
The slowly activating delayed rectifier K + current (I Ks ) is one of the main repolarizing currents in the human heart. Evidence has shown that angiotensin II (Ang II) regulates I Ks through the protein kinase C (PKC) pathway, but the related results are controversial. This study was designed to identify PKC isoenzymes involved in the regulation of I Ks by Ang II and the underlying molecular mechanism. The whole-cell patch-clamp technique was used to record I Ks in isolated guinea pig ventricular cardiomyocytes and in human embryonic kidney (HEK) 293 cells co-transfected with human KCNQ1/KCNE1 genes and Ang II type 1 receptor genes...
February 13, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29452138/exposure-to-low-level-of-lead-during-preweaning-period-increases-metallothionein-3-expression-and-dysregulates-divalent-cation-levels-in-the-brain-of-young-rats
#19
Abdur Rahman, Khalid M Khan, Muddanna S Rao
Lead (Pb) is a neurotoxic heavy metal, but the mechanism of its neurotoxicity is not clearly understood. Expression of metallothioneins (MTs) is induced in response to heavy metal exposure as a protective mechanism against heavy metal toxicity. There are several isoforms of MTs (MT-1 to 4), of which MT-3 is the neuron specific isoform, which also has neurite growth inhibitory effects. Whereas, the induction of MT-1 and 2 in response to Pb has been reported, the effect of Pb on the expression of MT-3 in the brain has not been documented...
February 13, 2018: Neurotoxicology
https://www.readbyqxmd.com/read/29451798/unraveling-the-nature-of-active-sites-in-ethanol-electro-oxidation-by-site-specific-marking-of-a-pt-catalyst-with-isotope-labeled-13co
#20
Manuel J S Farias, William Cheuquepán, Auro A Tanaka, Juan M Feliu
This works deals with the identification of preferential site-specific activation at a model Pt surface during a multi-product reaction. The (110)-type steps of a Pt(332) surface were selectively marked by attaching isotope-labeled 13CO molecules to them, and ethanol oxidation was probed by in situ FTIR in order to precisely determine the specific sites at which CO2, acetic acid, and acetaldehyde were preferentially formed. The (110) steps were active for splitting the C-C bond but, unexpectedly, we provide evidence that the pathway of CO2 formation was preferentially activated at (111) terraces, rather than at (110) steps...
February 16, 2018: Journal of Physical Chemistry Letters
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