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https://www.readbyqxmd.com/read/28647649/recovery-after-pilp-remineralization-of-dentin-lesions-created-with-two-cariogenic-acids
#1
K Saeki, Y-C Chien, G Nonomura, A F Chin, S Habelitz, L B Gower, S J Marshall, G W Marshall
OBJECTIVES: Acetate and lactate are important cariogenic acids produced by oral bacteria. They produced different residual dentin structures in artificial lesions of similar depth. We evaluated if such lesions responded in the same way to a polymer-induced-liquid-precursor (PILP) remineralization. DESIGN: Dentin blocks obtained from human third molars, divided into 6 groups (n=3). Blocks were demineralized with acetate (66h) or lactate (168h) buffer at pH 5.0 to create 140μm target lesion depths...
June 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28647614/inefficiency-of-ulipristal-acetate-on-uterus-leiomyomas-as-an-additional-sign-to-suspect-leiomyosarcoma
#2
Philippe Kadhel, Maïté Smail, Daphné Borja De Mozota
The unexpected diagnosis of a leiomyosarcoma from a surgical specimen of a presumed leiomyoma is rare, but with deleterious consequences, especially if morcellation techniques are used. There are currently no effective methods for such a preoperative diagnosis. Ulipristal acetate has demonstrated efficacy for the preoperative treatment of leiomyomas, but not for all patients. We report two cases of leiomyosarcomas in patients treated with ulipristal acetate for a presumed leiomyoma. We propose that ineffective ulipristal acetate treatment may indicate a population in which uterine leiomyosarcoma is more prevalent...
June 21, 2017: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/28647528/the-linkage-between-nutrient-supply-intracellular-enzyme-abundances-and-bacterial-growth-new-evidences-from-the-central-carbon-metabolism-of-corynebacterium-glutamicum
#3
Stephan Noack, Raphael Voges, Jochem Gätgens, Wolfgang Wiechert
Corynebacterium glutamicum serves as important production host for small molecular compounds that are derived from precursor molecules of the central carbon metabolism. It is therefore a well-studied model organism of industrial biotechnology. However, a deeper understanding of the regulatory principles underlying the synthesis of central metabolic enzymes under different environmental conditions as well as its impact on cell growth is still missing. We studied enzyme abundances in C. glutamicum in response to growth on: i) one limiting carbon source by sampling chemostat and fed-batch cultivations and ii) changing carbon sources provided in excess by sampling batch cultivations...
June 21, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28647504/antimicrobial-investigation-of-selected-soil-actinomycetes-isolated-from-unexplored-regions-of-kashmir-himalayas-india
#4
Aabid Manzoor Shah, Shakeel-U Rehman, Aehtesham Hussain, Saleem Mushtaq, Muzafar Ahmad Rather, Aiyatullah Shah, Zahoor Ahmad, Inshad Ali Khan, Khursheed Ahmad Bhat, Qazi Parvaiz Hassan
The aim of the present study was to isolate and evaluate the antimicrobial potential of soil actinomycetes of Kashmir Himalayas. The secondary metabolites of actinomycetes are the prominent source of antibiotics. A total of 121 morphologically different actinomycete strains were isolated and screened for antimicrobial activity against various human pathogens. The ethyl acetate extract of fermented broth an actinomycete strain, identified as Streptomyces pratensis exhibited significant antimicrobial activity against Staphylococcus aureus ATCC 29213 with MIC 0...
June 21, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28647447/dienogest-therapy-as-a-treatment-for-catamenial-pneumothorax-case-report-and-review-of-hormonal-options
#5
Shifana Lalani, Amanda Black, Meryl C Hodge, Togas Tulandi, Innie Chen
BACKGROUND: Catamenial pneumothorax is a rare but serious condition for women of reproductive age. We describe a trial of dienogest as hormonal therapy for catamenial pneumothorax and review the literature on hormonal suppressive therapy for this condition. CASE: A 39-year-old female, gravida 0 para 0, presented with recurrent pneumothoraces coinciding with her menses. After surgical therapy, she was started on leuprolide acetate injections for 6 months to reduce recurrence...
June 21, 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28647247/spatial-variability-and-possible-sources-of-acetate-and-formate-in-the-surface-snow-of-east-antarctica
#6
Gautami Samui, Runa Antony, Kanthanathan Mahalinganathan, Meloth Thamban
Spatial trends of acetate (Ac(-)) and formate (Fo(-)) were determined in surface snow samples along a coastal-inland transect (180km) in the ice cap region at Princess Elizabeth Land and along a coastal transect in the Amery Ice Shelf (130km), East Antarctica. Variations in both Ac(-) and Fo(-) seem to be unrelated to the acidity of snow. Ionic balance determined for the snow samples indicate the availability of HNO3 that could undergo photolysis to produce hydroxyl radical (OH), one of the major reactants involved in oxidation reactions with organic matter...
July 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28647122/effect-of-succinate-on-phosphate-solubilization-in-nitrogen-fixing-bacteria-harbouring-chick-pea-and-their-effect-on-plant-growth
#7
Bhagya Iyer, Mahendrapal Singh Rajput, Shalini Rajkumar
Diverse nitrogen fixing bacteria harbouring chick pea rhizosphere and root nodules were tested for multiple plant growth promoting traits like tricalcium phosphate (TCP) and rock phosphate (RP) solubilization, production of ammonia, indole 3-acetic acid, chitinase, phytase and alkaline phosphatase. Isolates belonged to diverse genus like Enterobacter, Acinetobacter, Erwinia, Pseudomonas, Rhizobium, Sinorhizobium, Ensifer, Klebsiella, etc. Most isolates solubilized TCP and RP along with the lowering of media pH, indicating acidification to be the chief mechanism behind this solubilization...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647117/isolation-of-a-potential-biocontrol-agent-paenibacillus-polymyxa-nsy50-from-vinegar-waste-compost-and-its-induction-of-host-defense-responses-against-fusarium-wilt-of-cucumber
#8
Nanshan Du, Lu Shi, Yinghui Yuan, Jin Sun, Sheng Shu, Shirong Guo
Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC) is one of the major destructive soil-borne diseases infecting cucumber. In this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. The 16S rDNA gene demonstrated that the biocontrol agents were Paenibacillus polymyxa (P. polymyxa), Bacillus amyloliquefaciens (B. amyloliquefaciens) and Bacillus licheniformis (B...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28646696/occurrence-of-the-most-common-helminth-infections-among-children-in-the-eastern-slovak-republic
#9
Jana Pipiková, Ingrid Papajová, Jindřich Šoltys, Ingrid Schusterová
OBJECTIVES: Ascariasis, trichuriasis and hymenolepiasis occur primarily within poor communities with low hygiene standards. This study examined the occurrence of intestinal helminth infections among children living in two counties (Košice and Prešov) in the Eastern Slovak Republic. STUDY DESIGN: Four hundred and twenty-six children were divided into groups according to ethnicity (non-Roma and Roma), age, sex, urban/rural residency and county of residence. METHODS: Stool samples collected from participants were processed by formalin-ethyl acetate sedimentation and examined microscopically...
June 21, 2017: Public Health
https://www.readbyqxmd.com/read/28646567/severe-menses-associated-hypertension-successfully-treated-with-gonadotropin-releasing-hormone-agonist
#10
Luke J Laffin, Munnam S Jafar, George L Bakris
A case of a 32-year-old nulliparous white woman referred for a 5-year history of severe hypertension, hypokalemia, and resultant systolic dysfunction is presented. She additionally had a left ventricular ejection fraction of 30% including left ventricular dilation and normal left ventricular mass index, as measured by cardiac magnetic resonance imaging when she initially presented to us. Her history revealed that her severe hypertension episodes were monthly and would occur around the catamenial (menses-associated) time...
June 23, 2017: Journal of Clinical Hypertension
https://www.readbyqxmd.com/read/28646418/in-vivo-nmr-as-a-tool-for-probing-molecular-structure-and-dynamics-in-intact-chlamydomonas-reinhardtii-cells
#11
Fatemeh Azadi-Chegeni, Christo Schiphorst, Anjali Pandit
We report the application of NMR dynamic spectral editing for probing the structure and dynamics of molecular constituents in fresh, intact cells and in freshly prepared thylakoid membranes of Chlamydomonas reinhardtii (Cr.) green algae. For isotope labeling, wild-type Cr. cells were grown on (13)C acetate-enriched minimal medium. 1D (13)C J-coupling based and dipolar-based MAS NMR spectra were applied to distinguish (13)C resonances of different molecular components. 1D spectra were recorded over a physiological temperature range, and whole-cell spectra were compared to those taken from thylakoid membranes, evaluating their composition and dynamics...
June 23, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28646332/a-rational-approach-for-the-improvement-of-biomass-production-and-lipid-profile-in-cacao-cell-suspensions
#12
Adriana María Gallego Rúa, Luisa Fernanda Rojas, Aura Ines Urrea Trujillo, Oriana Parra Zuleta, Cristian David Correa Alvarez, Lucía Atehortúa Garcés
Cocoa butter (CB) is produced in the seeds of Theobroma cacao representing 50% of its dry weight. The lipid composition plays an important role in the physicochemical, rheological, and sensory properties of the CB, making this fat a valuable resource for the production of chocolates, cosmetics, and pharmaceuticals. In this paper, are described experimental strategies used for a rational improvement of biomass production and fatty acids in cacao cell suspension cultures. First, the lipid profile in four cacao varieties is characterized, and then, one variety is selected to induce cell suspensions using a direct method without previous establishment of a callus phase...
June 23, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28645783/maerua-angolensis-stem-bark-extract-reverses-anxiety-and-related-behaviours-in-zebrafish-involvement-of-gabaergic-and-5-ht-systems
#13
Charles Kwaku Benneh, Robert Peter Biney, Priscilla Kolibea Mante, Augustine Tandoh, Donatus Wewura Adongo, Eric Woode
ETHNOPHARMACOLOGICAL RELEVANCE: Maerua angolensis DC (Capparaceae) has been employed in the management of several central nervous system (CNS) disorders including anxiety. This study evaluated the anxiolytic effects of the petroleum ether/ethyl acetate fraction stem bark extract and its possible mechanism(s) using zebrafish anxiety models. METHODS: Adult zebrafish, tested in the novel tank and light dark tests, have shown by previous authors to be sensitive to the anxiolytic effects of known anxiolytic drugs...
June 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28645652/androgen-inhibits-key-atherosclerotic-processes-by-directly-activating-adtrp-transcription
#14
Chunyan Luo, Elisabeth Pook, Bo Tang, Weiyi Zhang, Sisi Li, Kirsten Leineweber, Shing-Hu Cheung, Qiuyun Chen, Martin Bechem, Jing-Shan Hu, Volker Laux, Qing Kenneth Wang
Low androgen levels are associated with an increased risk of coronary artery disease (CAD), thrombosis and myocardial infarction (MI), suggesting that androgen has a protective role. However, little is known about the underlying molecular mechanism. Our genome-wide association study identified the ADTRP gene encoding the androgen-dependent TFPI regulating protein as a susceptibility gene for CAD and MI. The expression level of ADTRP was regulated by androgen, but the molecular mechanism is unknown. In this study, we identified the molecular mechanism by which androgen regulates ADTRP expression and tested the hypothesis that androgen plays a protective role in cardiovascular disease by activating ADTRP expression...
June 20, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28645106/phorbol-esters-dppa-dpa-promote-furin-expression-involving-transcription-factor-cebp%C3%AE-in-neuronal-cells
#15
Jing-Si Zha, Bing-Lin Zhu, Lu Liu, Yu-Jie Lai, Yan Long, Xiao-Tong Hu, Xiao-Juan Deng, Xue-Feng Wang, Zhen Yan, Guo-Jun Chen
Using high-throughput small molecule screening targeting furin gene, we identified that phorbol esters dPPA (12-Deoxyphorbol 13-phenylacetate 20-acetate) and dPA (12-Deoxyphorbol 13-acetate) significantly increased furin protein and mRNA expression in SH-SY5Y cells. This effect was prevented by PKC (protein kinase C) inhibitor calphostin C but not Ro318220, suggesting that the C1 domain, rather than the catalytic domain of PKC plays an important role. Luciferase assay revealed that nucleotides -7925 to -7426 were sufficient to mediate dPPA/dPA enhancement of furin P1 promoter activity...
June 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28645033/acetic-acid-assisted-fabrication-of-hierarchical-flower-like-bi2o3-for-photocatalytic-degradation-of-sulfamethoxazole-and-rhodamine-b-under-solar-irradiation
#16
Yueping Bao, Teik-Thye Lim, Ziyi Zhong, Rong Wang, Xiao Hu
With the assistance of acetic acid (CH3COOH), a novel 3D flower-like Bi2O3 was synthesized via hydrothermal process followed by calcination. For the first time, the role of CH3COOH as a capping agent in the formation of flower-like structure was investigated. The as-prepared flower-like Bi2O3 had a high activity on the degradation of sulfamethoxazole (SMX) under simulated solar light irradiation due to the narrow band gap of 2.69eV, high percentage of β-Bi2O3 as well as high intensity of polar facets (120) and (200)...
May 23, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28645021/development-a-validated-highly-sensitive-lc-ms-ms-method-for-simultaneous-quantification-of-ledipasvir-sofosbuvir-and-its-major-metabolite-gs-331007-in-human-plasma-application-to-a-human-pharmacokinetic-study
#17
Ola M Abdallah, Ahmed M Abdel-Megied, Amira S Gouda
A highly sensitive and rapid LC-MS/MS method was developed, fully optimized and validated for the simultaneous determination of Ledipasvir (LED) and Sofosbuvir (SOF) in the presence of its major metabolite GS-331007 in human plasma using Daclatasvir as internal standard (IS). The extraction of analytes and IS from plasma was performed using liquid-liquid extraction with ethyl acetate. The chromatographic separation of these prepared samples was achieved on Xterra MS C8 column (4.6×50mm,5μm) using gradient elution with a mobile phase of ammonium formate buffer (pH 3...
June 20, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28645006/study-of-intestinal-anti-inflammatory-activity-of-phoenix-loureiroi-kunth-arecaceae-fruit
#18
Rajan Murugan, Shanmugam Saravanan, Thangaraj Parimelazhagan
Phoenix loureiroi Kunth belongs to the family Arecaceae, the fruits are widely consumed by Kurumba and Irula tribes of Tamil Nadu (India) and also used to cure intestinal related diseases in folklore medicine. Therefore, in vivo animal studies were evaluated to confirm the prevention of inflammatory bowel disease (IBD) with the treatment of this plant fruits. The IBD was studied in Wistar albino rats by indomethacin (s.c.), acetic acid (i.c.) and dextran sulfate sodium (DSS) induced models. The diseases parameters such as macroscopic, microscopic features, serum biomarkers levels, haematological profile, biochemical and antioxidant levels were determined...
June 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28644711/screening-for-squamous-cell-anal-cancer-in-hiv-positive-patients-a-five-year-experience
#19
Chiara Santorelli, Cosimo Alex Leo, Jonathan D Hodgkinson, Franco Baldelli, Francesco Cantarella, Emanuel Cavazzoni
AIM: Potential screening modalities for early diagnosis of squamous cell anal cancer (SCC) in HIV patients include digital anorectal examination (DARE), anal Papanicolaou testing (Pap test), human papilloma virus (HPV) co-testing, and high-resolution anoscopy. The aim of this study was to demonstrate the results of a five-year screening program for SCC in HIV patients. MATERIALS AND METHODS: We conducted a retrospective study on 204 HIV patients who underwent a screening program for SCC from October 2010 to January 2015...
June 23, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/28644629/environmental-polymer-degradation-using-the-distonic-radical-ion-approach-to-study-the-gas-phase-reactions-of-model-polyester-radicals
#20
Bethany I Taggert, Richard A J O'Hair, Uta Wille
A novel precursor to the distonic O- and C-centred radical cations Oxo+O• and Oxo+C• was designed and synthesized, which represent model systems for radicals produced during polyester degradation. The precursor is equipped with a nitrate functional group, which serves as a masked site for these alkoxyl and carbon radicals that are unleashed through collision-induced dissociation (CID). Oxo+O• and Oxo+C• feature a cyclic carboxonium ion as permanent charge tag to enable monitoring their ion-molecule reactions on the millisecond to second timescale in the ion trap of the mass spectrometer...
June 23, 2017: Journal of Physical Chemistry. A
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