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Aldehyde dehydrogenase

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https://www.readbyqxmd.com/read/28807058/role-of-metabolites-of-cyclophosphamide-in-cardiotoxicity
#1
Koichiro Kurauchi, Takuro Nishikawa, Emiko Miyahara, Yasuhiro Okamoto, Yoshifumi Kawano
BACKGROUND: The dose-limiting toxic effect of cyclophosphamide (CY) is cardiotoxicity. The pathogenesis of myocardial damage is poorly understood, and there is no established means of prevention. In previous studies, we suggested that for CY-induced cardiotoxicity, whereas acrolein is the key toxic metabolite, carboxyethylphosphoramide mustard (CEPM) is protective. We sought to verify that acrolein is the main cause of cardiotoxicity and to investigate whether aldehyde dehydrogenase (ALDH), which is associated with greater CEPM production, is involved in the protective effect for cardiotoxicity...
August 14, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28799697/methylmalonate-semialdehyde-dehydrogenase-mediated-metabolite-homeostasis-essentially-regulate-conidiation-polarized-germination-and-pathogenesis-in-magnaporthe-oryzae
#2
Justice Norvienyeku, Zhenhui Zhong, Lili Lin, Xie Dang, Meilian Chen, Xiaolian Lin, Honghong Zhang, Wilfred Mabeche Anjago, Lianyu Lin, Waheed Abdul, Zonghua Wang
Plants generate multitude of aldehydes under abiotic and biotic stress conditions. Ample demonstrations have shown that rice-derived aldehydes enhance the resistance of rice against the rice-blast fungus Magnaporthe oryzae. However, how the fungal pathogen nullifies the inhibitory effects of host aldehydes to establish compatible interaction remains unknown. Here we identified and evaluated the in vivo transcriptional activities of M. oryzae aldehyde dehydrogenase (ALDH) genes. Transcriptional analysis of M...
August 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28797171/novel-composite-drug-delivery-system-as-a-novel-radio-sensitizer-for-the-local-treatment-of-cervical-carcinoma
#3
Shan Xu, Yu Ying Tang, Yan Xin Yu, Qin Yun, Jing Pin Yang, Heng Zhang, Qiuxia Peng, Xiaoyang Sun, Ling Lin Yang, ShaoZhi Fu, Jing Bo Wu
In this study, we investigated in vivo radiosensitizing effects of a gel-based dual drug delivery system (DDS) (PECE/DDP + mPEG-PCL/PTX, or PDMP) in a cervical cancer model, and determined its possible mechanisms of action. A xenograft cervical cancer model was used to investigate the radio sensitization effect of PDMP. Mice underwent paclitaxel (PTX) + cisplatin (DDP), PECE, or PDMP treatment followed by single radiation doses ranging from 0 Gy to 20 Gy. Radio sensitization was analyzed by tumor regrowth delay (TGD)...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28796245/dioscin-induces-prostate-cancer-cell-apoptosis-through-activation-of-estrogen-receptor-%C3%AE
#4
Xufeng Tao, Lina Xu, Lianhong Yin, Xu Han, Yan Qi, Youwei Xu, Shasha Song, Yanyan Zhao, Jinyong Peng
Recent researches have shown that estrogen receptor-β (ERβ) activator may be a potent anticancer agent for prostate cancer (PCa), and our previous study also indicated that dioscin can upregulate the expression of ERβ in MC3T3-E1 cell. In the present work, the activity and mechanism of dioscin, a natural product, against PCa were investigated. The results showed that dioscin markedly inhibited cell viability, colony formation, motility and induced apoptosis in PC3 cells. Moreover, dioscin disrupted the formation of PC3 cell-derived mammospheres and reduced aldehyde dehydrogenase (ALDH) level and the CD133(+)/CD44(+) cells, indicating that dioscin had a potent inhibitory activity on prostate cancer stem cells (PCSCs)...
August 10, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28792511/aldehyde-dehydrogenase-1-aldh1-isoform-expression-and-potential-clinical-implications-in-hepatocellular-carcinoma
#5
Cheng-Kun Yang, Xiang-Kun Wang, Xi-Wen Liao, Chuang-Ye Han, Ting-Dong Yu, Wei Qin, Guang-Zhi Zhu, Hao Su, Long Yu, Xiao-Guang Liu, Si-Cong Lu, Zhi-Wei Chen, Zhen Liu, Ke-Tuan Huang, Zheng-Tao Liu, Yu Liang, Jian-Lu Huang, Kai-Yin Xiao, Min-Hao Peng, Cheryl Ann Winkle, Stephen J O'Brien, Tao Peng
Hepatocellular carcinoma (HCC) is one of the most prevalent and life-threatening malignancies worldwide. There are few diagnostic and prognostic biomarkers and druggable targets for HCC. Aldehyde dehydrogenase 1 (ALDH1) is a marker of stem cells in a variety of cancers, but the mRNA levels and prognostic value of ALDH1 isoforms in HCC patients remain unknown. In the present study, gene ontology annotation of the ALDH1 family was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID), and the gene pathway analsis was performed using GeneMANIA software...
2017: PloS One
https://www.readbyqxmd.com/read/28791590/heterologous-expression-of-aldehyde-dehydrogenase-in-lactococcus-lactis-for-acetaldehyde-detoxification-at-low-ph
#6
Yunbin Lyu, Gisèle LaPointe, Lei Zhong, Jing Lu, Chong Zhang, Zhaoxin Lu
Aldehyde dehydrogenase (E.C. 1.2.1.x) can catalyze detoxification of acetaldehydes. A novel acetaldehyde dehydrogenase (istALDH) from the non-Saccharomyces yeast Issatchenkia terricola strain XJ-2 has been previously characterized. In this work, Lactococcus lactis with the NIsin Controlled Expression (NICE) System was applied to express the aldehyde dehydrogenase gene (istALDH) in order to catalyze oxidation of acetaldehyde at low pH. A recombinant L. lactis NZ3900 was obtained and applied for the detoxification of acetaldehyde as whole-cell biocatalysts...
August 8, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28769963/tissue-specific-floral-transcriptome-analysis-of-the-sexually-deceptive-orchid-chiloglottis-trapeziformis-provides-insights-into-the-biosynthesis-and-regulation-of-its-unique-uv-b-dependent-floral-volatile-chiloglottone-1
#7
Darren C J Wong, Ranamalie Amarasinghe, Claudia Rodriguez-Delgado, Rodney Eyles, Eran Pichersky, Rod Peakall
The Australian sexually deceptive orchid, Chiloglottis trapeziformis, employs a unique UV-B-dependent floral volatile, chiloglottone 1, for specific male wasp pollinator attraction. Chiloglottone 1 and related variants (2,5-dialkylcyclohexane-1,3-diones), represent a unique class of specialized metabolites presumed to be the product of cyclization between two fatty acid (FA) precursors. However, the genes involved in the biosynthesis of precursors, intermediates, and transcriptional regulation remains to be discovered...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28766688/stathmin1-expression-is-associated-with-aggressive-phenotypes-and-cancer-stem-cell-marker-expression-in-breast-cancer-patients
#8
Sayaka Obayashi, Jun Horiguchi, Toru Higuchi, Ayaka Katayama, Tadashi Handa, Bolag Altan, Tuya Bai, Pinjie Bao, Halin Bao, Takehiko Yokobori, Masahiko Nishiyama, Tetsunari Oyama, Hiroyuki Kuwano
Stathmin1 (STMN1) regulates progression in various cancers. The present study aimed to determine the relationship between STMN1 expression and several cancer-related markers in breast cancer. Using immunohistochemistry, we evaluated STMN1, estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, Ki-67, epidermal growth factor receptor (EGFR), CK5/6, CD44, CD24, aldehyde dehydrogenase 1, E-cadherin, epithelial cell adhesion molecule, and vimentin in 237 breast cancer patients and the clinical significance of STMN1...
September 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28766170/aegle-marmelos-differentially-affects-hepatic-markers-of-glycolysis-insulin-signalling-pathway-hypoxia-and-inflammation-in-hepg2-cells-grown-in-fructose-versus-glucose-rich-environment
#9
H Aggarwal, J Nair, P Sharma, R Sehgal, U Naeem, P Rajora, R Mathur
Fructose consumption is responsible for the onset of insulin resistance (IR), and metabolic syndrome. It possesses no functional utility in body and its detrimental effects on hepatic metabolic milieu are beyond those produced by glucose. The need of the hour is to identify fructose-induced IR as an unique pathological state to be managed differentially. The effect of aqueous leaf extract of Aegle marmelos (AM) on hepatic markers of insulin resistance using HepG2 cells cultured in either fructose or glucose-rich environment is investigated...
August 1, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28763253/alda-1-protects-against-acrolein-induced-acute-lung-injury-and-endothelial-barrier-dysfunction
#10
Qing Lu, Miles Mundy, Eboni Chambers, Thilo Lange, Julie Newton, Diana Borgas, Hongwei Yao, Gaurav Choudhary, Rajshekhar Basak, Mahogany Oldham, Sharon Rounds
Inhalation of acrolein, a highly reactive aldehyde, causes lung edema. The underlying mechanism is poorly understood and there is no effective treatment. In this study, we demonstrated that acrolein not only dose-dependently induced lung edema, but also promoted lipopolysaccharide (LPS)-induced acute lung injury (ALI). Importantly, acrolein-induced lung injury were prevented and rescued by Alda-1, an activator of mitochondrial aldehyde dehydrogenase (ALDH) 2. Acrolein also dose-dependently increased monolayer permeability, disrupted adherens junctions and focal adhesion complexes, and caused intercellular gap formation in primary cultured lung microvascular endothelial cells (LMVEC); effects that were attenuated by Alda-1 and the antioxidant, N-acetylcysteine, but not by the NADPH inhibitor, apocynin...
August 1, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28760736/targeting-long-non-coding-rna-dancr-inhibits-triple-negative-breast-cancer-progression
#11
Sha Sha, Dongya Yuan, Yuejun Liu, Baosan Han, Nanbert Zhong, Zhiqiang Liu
Triple negative breast cancer (TNBC) is non-responsive to conventional anti-hormonal and Her2 targeted therapies, making it necessary to identify new molecular targets for therapy. Long non-coding RNA anti-differentiation ncRNA (lncRNA DANCR) was identified in participating carcinogenesis of hepatocellular carcinoma, but its expression and potential role in TNBC progression is still unclear. In the present study, our results showed that DANCR expression was increased in TNBC tissues compared with the adjacent normal tissues using quantitative real-time PCR (qRT-PCR) in 63 TNBC specimens...
July 31, 2017: Biology Open
https://www.readbyqxmd.com/read/28759873/the-alterations-in-alcohol-dehydrogenase-and-aldehyde-dehydrogenase-activities-in-the-sera-of-patients-with-renal-cell-carcinoma
#12
Karolina Orywal, Wojciech Jelski, Tadeusz Werel, Maciej Szmitkowski
PURPOSE: In a previous study we showed that the total activity of alcohol dehydrogenase (ADH) and its isoenzyme class I was significantly higher in renal cancer (RCC) cells compared to normal kidney. The aim of this study was to compare the activities of ADH isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with different stages of RCC and healthy subjects. MATERIALS AND METHODS: Serum samples were taken from 54 patients with clear cell RCC (17 patients in stage II, 22 in stage III and 15 in stage IV) and 52 healthy patients...
July 28, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28751278/production-of-3-hydroxypropionic-acid-by-balancing-the-pathway-enzymes-using-synthetic-cassette-architecture
#13
Mugesh Sankaranarayanan, Ashok Somasundar, Eunhee Seol, Ashish Singh Chauhan, Seongjin Kwon, Gyoo Yeol Jung, Sunghoon Park
Biological 3-hydroxypropionic acid (3-HP) production from glycerol is a two-step reaction catalyzed by glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH). Recombinant strains developed for 3-HP production often suffer from the accumulation of a toxic intermediate, 3-hydroxypropionaldehyde (3-HPA). In order to avoid 3-HPA accumulation, balancing of the two enzymatic activities, in the present study, was attempted by employment of synthetic-regulatory cassettes comprising varying-strength promoters and bicistronic ribosome-binding sites (RBSs)...
July 24, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28750526/improved-sensitivity-of-acetaldehyde-biosensor-by-detecting-adh-reverse-reaction-mediated-nadh-fluoro-quenching-for-wine-evaluation
#14
Kenta Iitani, Po-Jen Chien, Takuma Suzuki, Koji Toma, Takahiro Arakawa, Yasuhiko Iwasaki, Kohji Mitsubayashi
Acetaldehyde (AcH) is found in ambient air, foods, and the living body. This toxic substance is also contained in wine and known as an important ingredient affecting the quality of wine. Herein, we constructed and evaluated two different fiber-optic biosensors for measurement of AcH in the liquid phase (AcH biosensor) using aldehyde dehydrogenase (ALDH) or alcohol dehydrogenase (ADH). The AcH biosensor measured a concentration of AcH using fluorescence intensity of a reduced form of nicotinamide adenine dinucleotide (NADH) that was produced or consumed via catalytic reaction of the respective enzyme...
July 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28749584/the-aldh21-gene-found-in-lower-plants-and-some-vascular-plants-codes-for-a-nadp-dependent-succinic-semialdehyde-dehydrogenase
#15
Martina Kopečná, Armelle Vigouroux, Jan Vilím, Radka Končitíková, Pierre Briozzo, Eva Hájková, Lenka Jašková, Klaus von Schwartzenberg, Marek Šebela, Solange Moréra, David Kopečný
Lower plant species including some green algae, non-vascular plants (bryophytes) as well as the oldest vascular plants (lycopods) and ferns (monilophytes) possess a unique aldehyde dehydrogenase (ALDH) gene named ALDH21, which is upregulated during dehydration. However, the gene is absent in flowering plants. Here, we show that ALDH21 from the moss Physcomitrella patens codes for a tetrameric NADP(+) -dependent succinic semialdehyde dehydrogenase (SSALDH), which converts succinic semialdehyde, an intermediate of the γ-aminobutyrate (GABA) shunt pathway, into succinate in the cytosol...
July 27, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28747008/enhanced-robustness-in-acetone-butanol-ethanol-fermentation-with-engineered-clostridium-beijerinckii-overexpressing-adhe2-and-ctfab
#16
Congcong Lu, Le Yu, Saju Varghese, Mingrui Yu, Shang-Tian Yang
Clostridium beijerinckii CC101 was engineered to overexpress aldehyde/alcohol dehydrogenase (adhE2) and CoA-transferase (ctfAB). Solvent production and acid assimilation were compared between the parental and engineered strains expressing only adhE2 (CC101-SV4) and expressing adhE2, ald and ctfAB (CC101-SV6). CC101-SV4 showed an early butanol production from glucose but stopped pre-maturely at a low butanol concentration of ∼6g/L. Compared to CC101, CC101-SV6 produced more butanol (∼12g/L) from glucose and was able to re-assimilate more acids, which prevented "acid crash" and increased butanol production, under all conditions studied...
July 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28744156/cholesterol-biosynthesis-inhibitor-ro-48-8071-reduces-progesterone-receptor-expression-and-inhibits-progestin-dependent-stem-cell-like-cell-growth-in-hormone-dependent-human-breast-cancer-cells
#17
Yayun Liang, Sandy Goyette, Salman M Hyder
Clinical trials and studies have shown that postmenopausal women undergoing combination hormone replacement therapy containing estrogen and progestin have an increased risk of breast cancer compared with women taking estrogen or placebo alone. Using animal models, we have previously shown that synthetic progestins, including medroxyprogesterone acetate (MPA), which is widely used clinically, accelerate breast cancer tumor growth and promote metastasis. Furthermore, we have found that MPA elevates CD44 protein expression and aldehyde dehydrogenase (ALDH) activity, two markers of cancer stem cells (CSCs), and increases mammosphere formation, another hallmark of stem cells, in hormone-dependent T47-D human breast cancer cells...
2017: Breast Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/28732729/crystal-structures-of-an-atypical-aldehyde-dehydrogenase-having-bidirectional-oxidizing-and-reducing-activities
#18
Kyoungho Jung, Seung-Hye Hong, Ho-Phuong-Thuy Ngo, Thien-Hoang Ho, Yeh-Jin Ahn, Deok-Kun Oh, Lin-Woo Kang
Aldehyde dehydrogenases (ALDHs) are NAD(P)(+)-dependent oxidoreductases that catalyze the oxidation of a variety of aldehydes to their acid forms. In this study, we determined the crystal structures of ALDH from Bacillus cereus (BcALDH), alone, and in complex with NAD(+) and NADP(+). This enzyme can oxidize all-trans-retinal to all-trans-retinoic acid using either NAD(+) or NADP(+) with equal efficiency, and atypically, as a minor activity, can reduce all-trans-retinal to all-trans-retinol using NADPH. BcALDH accommodated the additional 2'-phosphate of NADP(+) by expanding the cofactor-binding pocket and upshifting the AMP moiety in NADP(+)...
July 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28731185/snail-maintains-metastatic-potential-cancer-stem-like-properties-and-chemoresistance-in-mesenchymal-mouse-breast-cancer-tubo%C3%A2-p2j-cells
#19
Sun Young Ma, Jin-Hee Park, Hana Jung, Sung-Min Ha, Yeonye Kim, Dong Hyen Park, Deuk Hee Lee, Sooyong Lee, In-Ho Chu, So Young Jung, Il-Hwan Kim, Il-Whan Choi, Chang Soo Choi, Saegwang Park
Snail, a zinc-finger transcriptional repressor of E-cadherin expression, is one of the key inducers of epithelial-mesenchymal transition (EMT) in epithelial cancer. In breast cancer, EMT has been associated with malignancies, including metastasis, cancer stem-like properties, and resistance to chemotherapy and radiotherapy. In this study, we analysed the role of Snail in the highly metastatic mesenchymal TUBO‑P2J mouse breast cancer cells, by loss of function using short hairpin RNA. Though silencing Snail did not restore the E-cadherin expression or induce morphological changes, Snail silencing significantly ablated in vitro and in vivo metastatic potentials...
July 18, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28729482/brca1-and-brca2-tumor-suppressors-protect-against-endogenous-acetaldehyde-toxicity
#20
Eliana Mc Tacconi, Xianning Lai, Cecilia Folio, Manuela Porru, Gijs Zonderland, Sophie Badie, Johanna Michl, Irene Sechi, Mélanie Rogier, Verónica Matía García, Ankita Sati Batra, Oscar M Rueda, Peter Bouwman, Jos Jonkers, Anderson Ryan, Bernardo Reina-San-Martin, Joannie Hui, Nelson Tang, Alejandra Bruna, Annamaria Biroccio, Madalena Tarsounas
Maintenance of genome integrity requires the functional interplay between Fanconi anemia (FA) and homologous recombination (HR) repair pathways. Endogenous acetaldehyde, a product of cellular metabolism, is a potent source of DNA damage, particularly toxic to cells and mice lacking the FA protein FANCD2. Here, we investigate whether HR-compromised cells are sensitive to acetaldehyde, similarly to FANCD2-deficient cells. We demonstrate that inactivation of HR factors BRCA1, BRCA2, or RAD51 hypersensitizes cells to acetaldehyde treatment, in spite of the FA pathway being functional...
July 20, 2017: EMBO Molecular Medicine
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