keyword
https://read.qxmd.com/read/35536721/evaluation-of-spatially-targeted-scleral-stiffening-on-neuroprotection-in-a-rat-model-of-glaucoma
#21
JOURNAL ARTICLE
Brandon G Gerberich, Bailey G Hannon, Dillon M Brown, A Thomas Read, Matthew D Ritch, Elisa Schrader Echeverri, Lauren Nichols, Cahil Potnis, Sreesh Sridhar, Maya G Toothman, Stephen A Schwaner, Erin J Winger, Hannah Huang, Gabby S Gershon, Andrew J Feola, Machelle T Pardue, Mark R Prausnitz, C Ross Ethier
Purpose: Scleral stiffening may protect against glaucomatous retinal ganglion cell (RGC) loss or dysfunction associated with ocular hypertension. Here, we assess the potential neuroprotective effects of two treatments designed to stiffen either the entire posterior sclera or only the sclera adjacent to the peripapillary sclera in an experimental model of glaucoma. Methods: Rat sclerae were stiffened in vivo using either genipin (crosslinking the entire posterior sclera) or a regionally selective photosensitizer, methylene blue (stiffening only the juxtaperipapillary region surrounding the optic nerve)...
May 2, 2022: Translational Vision Science & Technology
https://read.qxmd.com/read/35513208/computational-modeling-of-corneal-and-scleral-collagen-photocrosslinking
#22
JOURNAL ARTICLE
Brandon G Gerberich, Amy J Wood-Yang, Afsane Radmand, Lauren M Nichols, Amir Hejri, Elisa Schrader Echeverri, Hannah G Gersch, Mark R Prausnitz
Scleral photocrosslinking is increasingly investigated for treatment of myopia and glaucoma. In this study a computational model was developed to predict crosslinking efficiency of visible/near infrared photosensitizers in the sclera. Photocrosslinking was validated against riboflavin corneal crosslinking experimental studies and subsequently modeled for the sensitizer, methylene blue, administered by retrobulbar injection to the posterior sclera and irradiated with a transpupillary light beam. Optimal ranges were determined for treatment parameters including light intensity, methylene blue concentration, injection volume, and inspired oxygen concentration...
May 2, 2022: Journal of Controlled Release
https://read.qxmd.com/read/34926791/thermostability-of-measles-and-rubella-vaccines-in-a-microneedle-patch
#23
JOURNAL ARTICLE
Jessica C Joyce, Marcus L Collins, Paul A Rota, Mark R Prausnitz
Measles and rubella vaccinations are highly effective at reducing disease prevalence; however, logistic issues related to subcutaneous administration and vaccine wastage limit the extent of vaccination coverage. Microneedle (MN) patches can increase coverage by easing logistics through simplified administration and improved stability. This study demonstrates the thermostability of a bivalent measles and rubella vaccine MN patch. Rubella vaccine stability required pH buffering during drying; potassium phosphate buffer at neutral pH was optimal for both vaccines...
October 2021: Advanced Therapeutics
https://read.qxmd.com/read/34921972/coaxial-electrospray-of-uniform-polylactide-core-shell-microparticles-for-long-acting-contraceptive
#24
JOURNAL ARTICLE
Jie Tang, Richard Schutzman, Carlos A Rodríguez, Joerg Lahann, Naír Rodríguez-Hornedo, Mark R Prausnitz, Steven P Schwendeman
Despite its high efficacy and good patient compliance, the only long-acting injectable (LAI) contraceptive currently available in the US, depot medroxyprogesterone acetate (DMPA), is limited by significant side effects and a delayed return to fertility for up to 10 months after its intended duration of action. To overcome these limitations, we sought to develop an injectable poly(D,l-lactide) (PLA) microparticle for sustained release of contraceptive hormone, etonogestrel (ENG). A one-step technique, coaxial electrospray method was applied to prepare uniform ENG loaded core-shell structured and slow-degrading PLA microparticles (ENG-cs-MPs) to provide release control while minimizing polymer content...
December 15, 2021: Journal of Controlled Release
https://read.qxmd.com/read/34744551/efficient-drug-delivery-into-skin-using-a-biphasic-dissolvable-microneedle-patch-with-water-insoluble-backing
#25
JOURNAL ARTICLE
Song Li, Dengning Xia, Mark R Prausnitz
Dissolvable microneedle patches (MNPs) enable simplified delivery of therapeutics via the skin. However, most dissolvable MNPs do not deliver their full drug loading to the skin because only some of the drug is localized in the microneedles (MNs), and the rest remains adhered to the patch backing after removal from the skin. In this work, biphasic dissolvable MNPs are developed by mounting water-soluble MNs on a water-insoluble backing layer. These MNPs enable the drug to be contained in the MNs without migrating into the patch backing due to the inability of the drugs to partition into the hydrophobic backing materials during MNP fabrication...
October 26, 2021: Advanced Functional Materials
https://read.qxmd.com/read/34670842/an-ultra-low-cost-electroporator-with-microneedle-electrodes-epatch-for-sars-cov-2-vaccination
#26
JOURNAL ARTICLE
Dengning Xia, Rui Jin, Gaurav Byagathvalli, Huan Yu, Ling Ye, Chao-Yi Lu, M Saad Bhamla, Chinglai Yang, Mark R Prausnitz
Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other pathogens with pandemic potential requires safe, protective, inexpensive, and easily accessible vaccines that can be developed and manufactured rapidly at a large scale. DNA vaccines can achieve these criteria, but induction of strong immune responses has often required bulky, expensive electroporation devices. Here, we report an ultra-low-cost (<1 USD), handheld (<50 g) electroporation system utilizing a microneedle electrode array ("ePatch") for DNA vaccination against SARS-CoV-2...
November 9, 2021: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/34605694/continuous-ketone-monitoring-consensus-report-2021
#27
JOURNAL ARTICLE
Kevin T Nguyen, Nicole Y Xu, Jennifer Y Zhang, Trisha Shang, Ananda Basu, Richard M Bergenstal, Kristin Castorino, Kong Y Chen, David Kerr, Suneil K Koliwad, Lori M Laffel, Nestoras Mathioudakis, L Kurt Midyett, Joshua D Miller, James H Nichols, Francisco J Pasquel, Priya Prahalad, Mark R Prausnitz, Jane Jeffrie Seley, Jennifer L Sherr, Elias K Spanakis, Guillermo E Umpierrez, Amisha Wallia, David C Klonoff
This article is the work product of the Continuous Ketone Monitoring Consensus Panel, which was organized by Diabetes Technology Society and met virtually on April 20, 2021. The panel consisted of 20 US-based experts in the use of diabetes technology, representing adult endocrinology, pediatric endocrinology, advanced practice nursing, diabetes care and education, clinical chemistry, and bioengineering. The panelists were from universities, hospitals, freestanding research institutes, government, and private practice...
October 4, 2021: Journal of Diabetes Science and Technology
https://read.qxmd.com/read/34597745/microneedle-patch-designs-to-increase-dose-administered-to-human-subjects
#28
JOURNAL ARTICLE
Wei Li, Song Li, Xiyi Fan, Mark R Prausnitz
Microneedle (MN) patches are being developed for many different kinds of drugs, but are often limited to delivering sub-milligram doses. This is because larger patches can be more difficult to apply to the skin, and acceptability of larger MN patches by human subjects has received limited study. Here, we fabricated 18 different large MN patch designs by laser microfabrication with different MN length (800-1500 μm), number of MNs (225 to 900 MNs per patch), space between MNs (600-1100 μm), and MN base diameter (200-250 μm)...
September 28, 2021: Journal of Controlled Release
https://read.qxmd.com/read/34589599/administration-of-pilocarpine-by-microneedle-patch-as-a-novel-method-for-cystic-fibrosis-sweat-testing
#29
JOURNAL ARTICLE
Song Li, Kelsey Hart, Natalie Norton, Clare A Ryan, Lokesh Guglani, Mark R Prausnitz
The sweat test is the gold standard for the diagnosis of cystic fibrosis (CF). The test utilizes iontophoresis to administer pilocarpine to the skin to induce sweating for measurement of chloride concentration in sweat. However, the sweat test procedure needs to be conducted in an accredited lab with dedicated instrumentation, and it can lead to inadequate sweat samples being collected in newborn babies and young children due to variable sweat production with pilocarpine iontophoresis. We tested the feasibility of using microneedle (MN) patches as an alternative to iontophoresis to administer pilocarpine to induce sweating...
September 2021: Bioengineering & Translational Medicine
https://read.qxmd.com/read/34480297/fabrication-of-pure-drug-microneedles-for-delivery-of-montelukast-sodium
#30
JOURNAL ARTICLE
Erkan Azizoglu, Ozgen Ozer, Mark R Prausnitz
Dissolving microneedle (MN) patches are usually formulated with a blend of drug and excipients added for mechanical strength and drug stabilization. In this study, we developed MNs made of pure drug to maximize drug loading capacity. MN patches were fabricated for transdermal delivery of montelukast sodium (MS) which is used to treat asthma and allergic rhinitis. We developed three different fabrication methods - solvent casting, melt casting, and solvent washing - and determined that filling molds with MS powder followed by a solvent washing method enabled MS to be loaded selectively to the MNs...
September 3, 2021: Drug Delivery and Translational Research
https://read.qxmd.com/read/34375689/immediate-detachment-of-microneedles-by-interfacial-fracture-for-sustained-delivery-of-a-contraceptive-hormone-in-the-skin
#31
JOURNAL ARTICLE
Yunki Lee, Wei Li, Jie Tang, Steven P Schwendeman, Mark R Prausnitz
Despite their high efficacy and safety, long-acting contraceptive methods are underutilized among women in some settings because they usually require injection or implantation by healthcare personnel. Here, we report a self-administrable microneedle (MN) patch for the rapid administration of a sustained-release contraceptive hormone delivery system into the skin that increases the simplicity and reliability of the MN delivery. We developed an immediate microneedle detachment system using a porous patch backing that has sufficient strength during MN insertion into skin under compression, but enables immediate detachment (< 1 s) of the MNs due to fracture at the MN - backing interface upon patch removal under tension from the skin surface...
August 7, 2021: Journal of Controlled Release
https://read.qxmd.com/read/34265331/development-of-a-thermostable-oxytocin-microneedle-patch
#32
JOURNAL ARTICLE
Yasmine Gomaa, Chandana Kolluru, Mikolaj Milewski, Dinah Lee, Jingtao Zhang, Robert Saklatvala, Mark R Prausnitz
Oxytocin is a nonapeptide hormone used in labor to initiate uterine contractions and to prevent and treat postpartum hemorrhage. Oxytocin is currently administered by injection and requires refrigerated transport and storage, which limits access, especially during home birth in developing countries. Here, we propose a thermostable, simple-to-administer microneedle (MN) patch for rapid delivery of oxytocin suitable for use by healthcare workers with limited training, like traditional birth attendants. Oxytocin (10 IU, 16...
September 10, 2021: Journal of Controlled Release
https://read.qxmd.com/read/34262052/immunologic-mechanisms-of-seasonal-influenza-vaccination-administered-by-microneedle-patch-from-a-randomized-phase-i-trial
#33
JOURNAL ARTICLE
Nadine G Rouphael, Lilin Lai, Sonia Tandon, Michele Paine McCullough, Yunchuan Kong, Sarah Kabbani, Muktha S Natrajan, Yongxian Xu, Yerun Zhu, Dongli Wang, Jesse O'Shea, Amy Sherman, Tianwei Yu, Sebastien Henry, Devin McAllister, Daniel Stadlbauer, Surender Khurana, Hana Golding, Florian Krammer, Mark J Mulligan, Mark R Prausnitz
In a phase 1 randomized, single-center clinical trial, inactivated influenza virus vaccine delivered through dissolvable microneedle patches (MNPs) was found to be safe and immunogenic. Here, we compare the humoral and cellular immunologic responses in a subset of participants receiving influenza vaccination by MNP to the intramuscular (IM) route of administration. We collected serum, plasma, and peripheral blood mononuclear cells in 22 participants up to 180 days post-vaccination. Hemagglutination inhibition (HAI) titers and antibody avidity were similar after MNP and IM vaccination, even though MNP vaccination used a lower antigen dose...
July 14, 2021: NPJ Vaccines
https://read.qxmd.com/read/34179728/skin-vaccination-with-dissolvable-microneedle-patches-incorporating-influenza-neuraminidase-and-flagellin-protein-nanoparticles-induces-broad-immune-protection-against-multiple-influenza-viruses
#34
JOURNAL ARTICLE
Ye Wang, Song Li, Chunhong Dong, Yao Ma, Yufeng Song, Wandi Zhu, Joo Kim, Lei Deng, Timothy L Denning, Sang-Moo Kang, Mark R Prausnitz, Bao-Zhong Wang
We generated self-adjuvanted protein nanoparticles of conserved influenza antigens and immunized mice via skin vaccination with dissolvable microneedle patches (MNPs) to increase the strength and breadth of immune responses. We produced M2e nanoparticles via ethanol desolvation, and double-layered NA1/M2e (shell/core), NA1-FliC/M2e, NA2/M2e, and NA2-FliC/M2e protein nanoparticles by chemically crosslinking influenza NA and flagellin (FliC) onto the surfaces of the M2e nanoparticles. The resulting nanoparticles retained FliC TLR5 innate signaling activity and significantly increased antigen-uptake and dendritic cell maturation in vitro ...
June 21, 2021: ACS Applied Bio Materials
https://read.qxmd.com/read/34175453/optimization-of-intracellular-macromolecule-delivery-by-nanoparticle-mediated-photoporation
#35
JOURNAL ARTICLE
Simple Kumar, Andrew Li, Naresh N Thadhani, Mark R Prausnitz
Nanoparticle-mediated photoporation is a novel delivery platform for intracellular molecule delivery. We studied the dependence of macromolecular delivery on molecular weight and sought to enhance delivery efficiency. DU145 prostate cancer cells were exposed to pulsed laser beam in the presence of carbon-black nanoparticles. Intracellular uptake of molecules decreased with increasing molecular weight. Attributing this dependence to molecular diffusivity, we hypothesized that macromolecular delivery efficiency could be enhanced by increasing either laser fluence or laser exposure duration at low fluence...
June 24, 2021: Nanomedicine: Nanotechnology, Biology, and Medicine
https://read.qxmd.com/read/34103912/serum-protects-cells-and-increases-intracellular-delivery-of-molecules-by-nanoparticle-mediated-photoporation
#36
JOURNAL ARTICLE
Simple Kumar, Eunice Lazau, Carter Kim, Naresh N Thadhani, Mark R Prausnitz
Introduction: Intracellular delivery of molecules is central to applications in biotechnology, medicine, and basic research. Nanoparticle-mediated photoporation using carbon black nanoparticles exposed to pulsed, near-infrared laser irradiation offers a physical route to create transient cell membrane pores, enabling intracellular delivery. However, nanoparticle-mediated photoporation, like other physical intracellular delivery technologies, necessitates a trade-off between achieving efficient uptake of exogenous molecules and maintaining high cell viability...
2021: International Journal of Nanomedicine
https://read.qxmd.com/read/33915696/dissolvable-microneedle-patches-to-enable-increased-access-to-vaccines-against-sars-cov-2-and-future-pandemic-outbreaks
#37
JOURNAL ARTICLE
Jesse O'Shea, Mark R Prausnitz, Nadine Rouphael
Vaccines are an essential component of pandemic preparedness but can be limited due to challenges in production and logistical implementation. While vaccine candidates were rapidly developed against severe acute respiratory syndrome coronavirus 2 (SARS-COV-2), immunization campaigns remain an obstacle to achieving herd immunity. Dissolvable microneedle patches are advantageous for many possible reasons: improved immunogenicity; dose-sparing effects; expected low manufacturing cost; elimination of sharps; reduction of vaccine wastage; no need for reconstitution; simplified supply chain, with reduction of cold chain supply through increased thermostability; ease of use, reducing the need for healthcare providers; and greater acceptability compared to traditional hypodermic injections...
April 1, 2021: Vaccines
https://read.qxmd.com/read/33908888/physician-stress-during-electronic-health-record-inbox-work-in-situ-measurement-with-wearable-sensors
#38
JOURNAL ARTICLE
Fatema Akbar, Gloria Mark, Stephanie Prausnitz, E Margaret Warton, Jeffrey A East, Mark F Moeller, Mary E Reed, Tracy A Lieu
BACKGROUND: Increased work through electronic health record (EHR) messaging is frequently cited as a factor of physician burnout. However, studies to date have relied on anecdotal or self-reported measures, which limit the ability to match EHR use patterns with continuous stress patterns throughout the day. OBJECTIVE: The aim of this study is to collect EHR use and physiologic stress data through unobtrusive means that provide objective and continuous measures, cluster distinct patterns of EHR inbox work, identify physicians' daily physiologic stress patterns, and evaluate the association between EHR inbox work patterns and physician physiologic stress...
April 28, 2021: JMIR Medical Informatics
https://read.qxmd.com/read/33590465/fabrication-of-microneedle-patches-with-lyophilized-influenza-vaccine-suspended-in-organic-solvent
#39
JOURNAL ARTICLE
Yoo Chun Kim, Jeong Woo Lee, E Stein Esser, Haripriya Kalluri, Jessica C Joyce, Richard W Compans, Ioanna Skountzou, Mark R Prausnitz
Skin vaccination by microneedle (MN) patch simplifies the immunization process to increase access to vaccines for global health. Lyophilization has been widely used to stabilize vaccines and other biologics during storage, but is generally not compatible with the MN patch manufacturing processes. In this study, our goal was to develop a method to incorporate lyophilized inactivated H1N1 influenza vaccine into MN patches during manufacturing by suspending freeze-dried vaccine in anhydrous organic solvent during the casting process...
February 15, 2021: Drug Delivery and Translational Research
https://read.qxmd.com/read/33511001/drug-free-nonsurgical-reduction-of-intraocular-pressure-for-four-months-after-suprachoroidal-injection-of-hyaluronic-acid-hydrogel
#40
JOURNAL ARTICLE
J Jeremy Chae, Jae Hwan Jung, Wei Zhu, Brandon G Gerberich, Mohammad Reza Bahrani Fard, Hans E Grossniklaus, C Ross Ethier, Mark R Prausnitz
Glaucoma is the leading cause of irreversible blindness. Current treatments use drugs or surgery to reduce intraocular pressure (IOP). In this study, a drug-free, nonsurgical method is developed that lowers IOP for 4 months without requiring daily patient adherence. The approach involves expanding the suprachoroidal space (SCS) of the eye with an in situ-forming hydrogel injected using a microneedle. This study tests the hypothesis that SCS expansion increases the drainage of aqueous humor from the eye via the unconventional pathway, which thereby lowers IOP...
January 2021: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
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