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Bone Harvesting

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https://www.readbyqxmd.com/read/27923216/efficacy-of-a-cellular-bone-allograft-for-foot-and-ankle-arthrodesis-and-revision-nonunion-procedures
#1
Travis J Dekker, Peter White, Samuel B Adams
BACKGROUND: Bone graft substitutes are often required in patients at risk for nonunion, and therefore, an allograft that most closely mimics an autograft is highly sought after. This study explored the utility and efficacy of a cellular bone allograft used for foot and ankle arthrodesis and revision nonunion procedures in a patient population at risk for nonunion. METHODS: An institutional review board-approved retrospective review of consecutive patients who underwent arthrodesis and revision nonunion procedures with a cellular bone allograft was performed at a single academic institution...
October 23, 2016: Foot & Ankle International
https://www.readbyqxmd.com/read/27916375/the-role-of-the-adaptive-immune-system-in-burn-induced-heterotopic-ossification-and-mesenchymal-cell-osteogenic-differentiation
#2
Kavitha Ranganathan, Shailesh Agarwal, David Cholok, Shawn Loder, Jonathan Li, Hsiao Hsin Sung Hsieh, Stewart C Wang, Steven R Buchman, Benjamin Levi
BACKGROUND: Heterotopic ossification (HO) is the pathologic process of extraskeletal bone formation. Although the exact etiology remains unknown, inflammation appears to catalyze disease progression. The goal of this study is to determine the impact of the adaptive immune system on HO. METHODS: HO was induced in 8-wk-old control C57BL/6 and immunocompromised Rag1tm1Mom (Rag1 KO) male mice deficient in B- and T-lymphocytes via combined Achilles tenotomy and burn injury...
November 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27916351/circumflex-scapular-artery-myocutaneous-and-or-vascularized-scapular-chimeric-flap
#3
Arzu Akcal, Tahsin Gorgulu, Seckin Aydin Savas, Mehmet Af Akcal, Mehmet Can Ubur, Cumhur Ibrahim Bassorgun
BACKGROUND: Chimeric osteomyocutaneous flaps harvested from the subscapular artery system have been used in clinical practice. We describe the use of a novel circumflex scapular artery myocutaneous and/or vascularized scapular chimeric flap in a rat model and demonstrate optimal skin flap dimensions. MATERIALS AND METHODS: An 8 × 4-cm-rectangular skin flap based on the circumflex scapular artery flap was harvested, and the mean percentage of the surviving flap area and the necrotic area were calculated to be 71% ± 17...
November 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27913902/a-new-multiscale-micromechanical-model-of-vertebral-trabecular-bones
#4
Rami Haj-Ali, Eyass Massarwa, Jacob Aboudi, Fabio Galbusera, Uwe Wolfram, Hans-Joachim Wilke
A new three-dimensional (3D) multiscale micromechanical model has been suggested as adept at predicting the overall linear anisotropic mechanical properties of a vertebral trabecular bone (VTB) highly porous microstructure. A nested 3D modeling analysis framework spanning the multiscale nature of the VTB is presented herein. This hierarchical analysis framework employs the following micromechanical methods: the 3D parametric high-fidelity generalized method of cells (HFGMC) as well as the 3D sublaminate model...
December 2, 2016: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/27912213/histomorphometric-evaluation-of-two-different-bone-substitutes-in-sinus-augmentation-procedures-a-randomized-controlled-trial-in-humans
#5
Ruggero Rodriguez Baena, Roberta Pastorino, Enrico Felice Gherlone, Letizia Perillo, Saturnino Marco Lupi, Alessandra Lucchese
PURPOSE: The histomorphometric results of sinus floor augmentation with deproteinized bovine bone (DBB) and a new fully synthetic bone substitute, poly(lactic-co-glycolic acid/hydroxyapatite) (PLGA/HA), were compared in humans. MATERIALS AND METHODS: Twelve maxillary sinuses of eight patients requiring major maxillary sinus floor augmentation and free of concomitant conditions (ASA scores 1 and 2) were studied. Lateral sinus augmentation was performed using DBB or PLGA/HA grafts; sites were randomly assigned to control or test groups...
December 2, 2016: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/27910011/chromosome-preparation-for-acute-lymphoblastic-leukemia
#6
Mary Shago
The chromosome abnormalities observed in acute lymphoblastic leukemia have been demonstrated to contribute to patient management and treatment stratification. This chapter provides a basic protocol for the procurement, culture, harvest, and slide preparation of bone marrow aspirate and unstimulated peripheral blood specimens.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909674/minimally-invasive-quadriceps-tendon-harvest-and-graft-preparation-for-all-inside-anterior-cruciate-ligament-reconstruction
#7
Harris S Slone, William B Ashford, John W Xerogeanes
The quadriceps tendon is an infrequently used graft option for anterior cruciate ligament reconstruction despite favorable clinical results in the literature with low donor site morbidity. It is a versatile graft that can be harvested with bone or as a soft tissue graft alone. In addition, it can be used for anatomic, double-bundle, transtibial, and all-inside reconstructions. The unique characteristics of the quadriceps tendon allow for the ability to harvest a single-bundle large-diameter graft and minimize disruption of normal anatomy, which makes the quadriceps tendon an excellent choice for all-inside techniques...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909665/autologous-osteophyte-grafting-for-open-wedge-high-tibial-osteotomy
#8
Takenori Akiyama, Ken Okazaki, Taro Mawatari, Satoshi Ikemura, Shunsuke Nakamura
Osteophytes are physiological bony outgrowths that develop at the margins of the articular surfaces during the progression of osteoarthritis; they are associated with active endochondral bone formation processes and expressions of various growth factors. We believe they could be a source of bone grafts as a result of a potentially strong osteoinductive effect. Moreover, osteophytes can be easily harvested by arthroscopy in patients undergoing open-wedge high tibial osteotomy (OW-HTO) for medial unicompartmental knee osteoarthritis...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27907194/preservation-of-multiple-mammalian-tissues-to-maximize-science-return-from-ground-based-and-spaceflight-experiments
#9
Sungshin Choi, Hami E Ray, San-Huei Lai, Joshua S Alwood, Ruth K Globus
BACKGROUND: Even with recent scientific advancements, challenges posed by limited resources and capabilities at the time of sample dissection continue to limit the collection of high quality tissues from experiments that can be conducted only infrequently and at high cost, such as in space. The resources and time it takes to harvest tissues post-euthanasia, and the methods and duration of long duration storage, potentially have negative impacts on sample quantity and quality, thereby limiting the scientific outcome that can be achieved...
2016: PloS One
https://www.readbyqxmd.com/read/27904498/pre-degenerated-peripheral-nerves-co-cultured-with-bone-marrow-derived-cells-a-new-technique-for-harvesting-high-purity-schwann-cells
#10
Xiao-Pan Wang, Min Wu, Jian-Zhong Guan, Zhao-Dong Wang, Xu-Bin Gao, Yang-Yang Liu
Schwann cells play an important role in the peripheral nervous system, especially in nerve repair following injury, so artificial nerve regeneration requires an effective technique for obtaining purified Schwann cells. In vivo and in vitro pre-degeneration of peripheral nerves have been shown to obtain high-purity Schwann cells. We believed that in vitro pre-degeneration was simple and controllable, and available for the clinic. Thus, we co-cultured the crushed sciatic nerves with bone marrow-derived cells in vitro...
October 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27903590/anterior-cruciate-ligament-derived-stem-cells-transduced-with-bmp2-accelerate-graft-bone-integration-after-acl-reconstruction
#11
Yohei Kawakami, Koji Takayama, Tomoyuki Matsumoto, Ying Tang, Bing Wang, Yutaka Mifune, James H Cummins, Ryan J Warth, Ryosuke Kuroda, Masahiro Kurosaka, Freddie H Fu, Johnny Huard
BACKGROUND: Strong graft-bone integration is a prerequisite for successful graft remodeling after reconstruction of the anterior cruciate ligament (ACL) using soft tissue grafts. Novel strategies to accelerate soft tissue graft-bone integration are needed to reduce the need for bone-tendon-bone graft harvest, reduce patient convalescence, facilitate rehabilitation, and reduce total recovery time after ACL reconstruction. HYPOTHESIS: The application of ACL-derived stem cells with enhanced expression of bone morphogenetic protein 2 (BMP2) onto soft tissue grafts in the form of cell sheets will both accelerate and improve the quality of graft-bone integration after ACL reconstruction in a rat model...
November 30, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27897302/structure-and-properties-of-various-hybrids-fabricated-by-silk-nanofibrils-and-nanohydroxyapatite
#12
Ruixin Mi, Yingxin Liu, Xin Chen, Zhengzhong Shao
To harvest silk fibroin (SF) based organic/inorganic composites with various general properties (e.g. hard or soft), the strategies of vacuum filtration and centrifugation were employed in this work to produce a film and hydrogel of SF-nanofibril/nanohydroxyapatite, respectively. It was found that the SF-nanofibril mediated the mineralization of hydroxyapatites (HAP) in situ and the morphology of such organic/inorganic nanohybrids presented a "flower-like" structure, mainly because of the strong interaction between SF-nanofibrils and nanohydroxyapatites...
November 29, 2016: Nanoscale
https://www.readbyqxmd.com/read/27895892/sexual-dimorphism-in-the-mast-cell-transcriptome-and-the-pathophysiological-responses-to-immunological-and-psychological-stress
#13
Emily Mackey, Saravanan Ayyadurai, Calvin S Pohl, Susan D' Costa, Yihang Li, Adam J Moeser
BACKGROUND: Biological sex plays a prominent role in the prevalence and severity of a number of important stress-related gastrointestinal and immune-related diseases including IBS and allergy/anaphylaxis. Despite the establishment of sex differences in these diseases, the underlying mechanisms contributing to sex differences remain poorly understood. The objective of this study was to define the role of biological sex on mast cells (MCs), an innate immune cell central to the pathophysiology of many GI and allergic disorders...
2016: Biology of Sex Differences
https://www.readbyqxmd.com/read/27894174/modified-iliac-crest-reconstruction-with-bone-cement-for-reduction-of-donor-site-pain-and-morbidity-after-open-wedge-high-tibial-osteotomy-a-prospective-study
#14
Jong Seong Lee, Yong Jee Park, Lih Wang, Yong Suk Chang, Gautam M Shetty, Kyung Wook Nha
Purpose: This study was to determine the efficacy of iliac crest reconstruction using bone cement in reducing pain and morbidity at the donor site in patients undergoing open wedge high tibial osteotomy (OWHTO) with tricortical iliac crest autologous graft. Materials and Methods: Thirty-three patients who underwent iliac crest reconstruction using polymethyl methacrylate (PMMA) bone cement (group A) and thirty patients who had no iliac crest reconstruction (group B) were enrolled in this study...
December 1, 2016: Knee Surgery & related Research
https://www.readbyqxmd.com/read/27893560/cranial-bone-graft-donor-site-reconstruction
#15
Muzaffer Çelik
My most important concern, in my entire experience with cranial bone grafting procedures, is managing the bone graft donor site such as donor site cavity from harvesting and weakness of the cranium. The most common patient complaint, following cranial bone grafting for aesthetic indications, is the presence of a cavity at the donor site. The authors have managed more than 200 patients since 2001, wherein the cranial bone graft-donor sites were reconstructed with tiny bone chip lamellae harvested from the area adjacent to the donor site...
November 23, 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/27886022/fracture-risk-of-vertebral-bodies-after-cryosurgery-using-a-miniature-cryoprobe-a-biomechanical-in-vitro-analysis-on-human-bones
#16
K Zarghooni, L Westermann, R Sobottke, M Röllinghoff, J Bredow, J Siewe, Peer Eysel, M J Scheyerer
INTRODUCTION: Due to spinal instability and compressive neurologic deficits surgical management is sometimes necessary in patients with metastatic spinal lesions. However, in some cases open surgery is not possible and minimally invasive procedures, like cryoablation, are needed. The aim of the current study was to investigate whether a miniature cryoprobe provides adequate tissue cooling in vertebrae and to evaluate the direct impact of cryosurgery on vertebral body stability. MATERIALS AND METHODS: Twelve thoracic vertebral bodies were harvested from fresh cadavers...
November 18, 2016: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
https://www.readbyqxmd.com/read/27882438/histochemical-examination-of-systemic-administration-of-eldecalcitol-combined-with-guided-bone-regeneration-for-bone-defect-restoration-in-rats
#17
Xiuchun Han, Juan Du, Di Liu, Hongrui Liu, Norio Amizuka, Minqi Li
The aim of this experiment was to elucidate the histological alterations after systemic administration of eldecalcitol (ELD) combined with guided bone regeneration during the restoration of bone defect healing in rats. The femurs of 8-week-old Wister rats were used to generate bone defect models. The defect was covered with a collagen membrane, and ELD group was administrated with eldecalcitol (50 ng/kg body weight) intragastrically once every other day. Femora were harvested at 1, 2, 4 and 8 weeks post-surgery...
November 23, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27879598/alveolar-bone-grafting-and-cleft-lip-and-palate-a-review
#18
E Hope Weissler, Kaitlyn M Paine, Mairaj K Ahmed, Peter J Taub
Alveolar bone grafting in the mixed dentition stage is an accepted step in the management of cleft alveoli, providing maxillary arch support for dentition and mastication. Points of contention regarding best practices remain, including specific timing, perioperative orthodontic management, bone harvest and substitutes, pain management, and outcomes evaluations. SCOPUS and MEDLINE were searched for articles about alveolar bone grafting, which were read independently by two authors and selected for inclusion on the basis of relevance and merit...
December 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27876575/effects-of-collagen-membrane-application-and-cortical-bone-perforation-on-de-novo-bone-formation-in-periosteal-distraction-an-experimental-study-in-a-rabbit-calvaria
#19
Ken Nakahara, Maiko Haga-Tsujimura, Kosaku Sawada, Matthias Mottini, Benoit Schaller, Nikola Saulacic
OBJECTIVES: The aim of the present study was to assess the impact of collagen membrane application and cortical bone perforations in periosteal distraction osteogenesis. STUDY DESIGN: A total of 32 New Zealand rabbits were randomized into four experimental groups, considering two treatment modalities. Calvarial bone was perforated or left intact (P+/-). In half the animals, the distraction mesh was covered with a collagen membrane (M+/-). All animals were subjected to a 7-day latency period and a 10-day distraction period...
September 28, 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/27876350/a-solution-to-the-vessel-shortage-during-free-vascularized-fibular-grafting-for-reconstructing-infected-bone-defects-of-the-femur-bridging-with-vein-transplantation
#20
Ren Gao-Hong, Li Run-Guang, Jiang Gui-Yong, Chen Chao-Jie, Bao Zhi-Gang
PURPOSE: The present study aimed to evaluate the feasibility and clinical efficacy of bridging vein transplantation to deal with the vessel shortage during free vascularized fibular grafting for reconstructing infected bone defects of the femur. METHODS: Twelve patients (aged 15-58 years) with infected bone defects of the femur (between 6.0 and 18.0cm) were recruited in this study. Vacuum sealing drainage were applied after extensive debridement of the infected bone defects and irrigated with 0...
October 22, 2016: Injury
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