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https://www.readbyqxmd.com/read/28427840/aav-id-a-rapid-and-robust-assay-for-batch-to-batch-consistency-evaluation-of-aav-preparations
#1
Simon Pacouret, Mohammed Bouzelha, Rajani Shelke, Eva Andres-Mateos, Ru Xiao, Anna Maurer, Mathieu Mevel, Heikki Turunen, Trisha Barungi, Magalie Penaud-Budloo, Frédéric Broucque, Véronique Blouin, Philippe Moullier, Eduard Ayuso, Luk H Vandenberghe
Adeno-associated virus (AAV) vectors are promising clinical candidates for therapeutic gene transfer, and a number of AAV-based drugs may emerge on the market over the coming years. To insure the consistency in efficacy and safety of any drug vial that reaches the patient, regulatory agencies require extensive characterization of the final product. Identity is a key characteristic of a therapeutic product, as it ensures its proper labeling and batch-to-batch consistency. Currently, there is no facile, fast, and robust characterization assay enabling to probe the identity of AAV products at the protein level...
April 17, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28373070/disulfiram-suppresses-cancer-stem-like-properties-and-stat3-signaling-in-triple-negative-breast-cancer-cells
#2
Yoon-Jae Kim, Ji Young Kim, Nahyun Lee, Eunhye Oh, Daeil Sung, Tae-Min Cho, Jae Hong Seo
In the presence of copper (Cu), disulfiram (DSF) suppresses properties associated with cancer stem cells (CSCs) in breast cancer, but the mechanism of action is poorly understood. In the present study, we observed that DSF/Cu treatment induced apoptosis, mediated by caspase-3 activation in triple-negative breast cancer (TNBC) cells. DSF/Cu treatment also specifically targeted CSC-like cell populations, marked by the inhibition of ALDH1 activity, the suppression of CD44+/CD24-and CD49f+/CD24 + subpopulations, and the subsequent impairment of mammosphere formation...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28371560/differential-scanning-calorimetry-and-fluorimetry-measurements-of-monoclonal-antibodies-and-reference-proteins-effect-of-scanning-rate-and-dye-selection
#3
Brian E Lang, Kenneth D Cole
Differential scanning calorimetry (DSC) and differential scanning fluorimetry (DSF) were used to measure the transition temperatures of four proteins: RNase A, invertase, rituximab, and the NISTmAb (NIST Reference Material, RM 8671). The proteins were combined with several different fluorescent dyes for the DSF measurements. This study compares the results of DSC and DSF measurements of transition temperatures with different types of proteins, dye combinations, and thermal scan rates. As protein unfolding is often influenced by kinetic effects, we measured the transition temperatures of the proteins using DSC over a range of temperature scan rates and compared them to the data obtained from DSF over comparable temperature scan rates...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28369120/fatty-acid-dsf-binds-and-allosterically-activates-histidine-kinase-rpfc-of-phytopathogenic-bacterium-xanthomonas-campestris-pv-campestris-to-regulate-quorum-sensing-and-virulence
#4
Zhen Cai, Zhi-Hui Yuan, Huan Zhang, Yue Pan, Yao Wu, Xiu-Qi Tian, Fang-Fang Wang, Li Wang, Wei Qian
As well as their importance to nutrition, fatty acids (FA) represent a unique group of quorum sensing chemicals that modulate the behavior of bacterial population in virulence. However, the way in which full-length, membrane-bound receptors biochemically detect FA remains unclear. Here, we provide genetic, enzymological and biophysical evidences to demonstrate that in the phytopathogenic bacterium Xanthomonas campestris pv. campestris, a medium-chain FA diffusible signal factor (DSF) binds directly to the N-terminal, 22 amino acid-length sensor region of a receptor histidine kinase (HK), RpfC...
April 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28355693/-influence-of-occupational-aluminum-exposure-on-cognitive-function-and-glutamate-receptor-protein-expression-in-workers
#5
P Ren, R Li, Y Z Yuan, X T Lu, Q Niu
Objective: To investigate the influence of occupational aluminum exposure on cognitive function and glutamate receptor protein expression in peripheral blood lymphocytes in workers and the possibility of glutamate receptor being used as a biomarker for cognitive impairment in aluminum workers. Methods: From October to December, 2014, cluster sampling was performed to select 121 workers in aluminum electrolysis workshop as exposure group and 231 workers in thermoelectric workshop and logistics department as control group...
February 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28351836/structural-and-biophysical-analyses-of-the-skeletal-dihydropyridine-receptor-beta-subunit-%C3%AE-1a-reveal-critical-roles-of-domain-interactions-for-stability
#6
Nicole C Norris, Soumya Joseph, Shouvik Aditya, Yamuna Karunasekara, Philip G Board, Angela F Dulhunty, Aaron J Oakley, Marco G Casarotto
Excitation-contraction (EC) coupling in skeletal muscle requires a physical interaction between the voltage-gated calcium channel dihydropyridine receptor (DHPR) and the ryanodine receptor (RyR1) Ca2+ release channel. Although the exact molecular mechanism that initiates skeletal EC coupling is unresolved, it is clear that both the α1s and β subunits of DHPR are essential for this process. Here, we employed a series of techniques, including size-exclusion chromatography-multi-angle light scattering, differential scanning fluorimetry and isothermal calorimetry, to characterize various biophysical properties of the skeletal DHPR beta subunit β1a...
March 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28344087/subacute-alcohol-and-or-disulfiram-intake-affects-bioelements-and-redox-status-in-rat-testes
#7
Ana Djuric, Aida Begic, Borko Gobeljic, Ana Pantelic, Goran Zebic, Ivana Stevanovic, Dragan Djurdjevic, Milica Ninkovic, Vera Prokic, Ivan Stanojevic, Danilo Vojvodic, Mirjana Djukic
The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C21/C42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A1-21 groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A21+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol)...
March 24, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28343267/tumor-associated-macrophages-and-regulatory-t-cells-infiltration-and-the-clinical-outcome-in-colorectal-cancer
#8
Dariusz Waniczek, Zbigniew Lorenc, Mirosław Śnietura, Mariusz Wesecki, Agnieszka Kopec, Małgorzata Muc-Wierzgoń
The aim of the study is the assessment of the intensity of the infiltration of tumor-associated macrophages (TAMs) CD68(+)/iNOS(-) and Tregs CD8(+)/FoxP3(+) in colorectal cancer (CRC) patients as prognostic factors with respect to disease-free survival (DFS) and overall survival (OS). In this retrospective study, tissue samples were obtained from 89 patients undergoing resection for CRC (stage IIA, pT3N0M0 and stages IIIB and IIIC, pT3N1-2M0). Recurrence was observed in 45 patients at the time of the follow-up (10 local recurrences, 35 distant metastases)...
March 25, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28340172/repositioning-disulfiram-as-a-radiosensitizer-against-atypical-teratoid-rhabdoid-tumor
#9
Young Eun Lee, Seung Ah Choi, Pil Ae Kwack, Hak Jae Kim, Il Han Kim, Kyu-Chang Wang, Ji Hoon Phi, Ji Yeoun Lee, Sangjoon Chong, Sung-Hye Park, Kyung Duk Park, Do Won Hwang, Kyeung Min Joo, Seung-Ki Kim
Background.: Atypical teratoid/rhabdoid tumor (AT/RT) is one of the most common malignant brain tumors in infants. Although cancer stem cells of AT/RT express aldehyde dehydrogenase (ALDH), effective chemotherapies against AT/RT have not been established. Here, we examined radiosensitizing effects of disulfiram (DSF), an irreversible inhibitor of ALDH against AT/RT for a novel therapeutic method. Methods.: Patient-derived primary cultured AT/RT cells (SNU.AT/RT-5 and SNU...
March 17, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28317003/peri-operative-inflammatory-cytokines-in-plasma-of-the-elderly-correlate-in-prospective-study-with-postoperative-changes-in-cognitive-test-scores
#10
R Kline, E Wong, M Haile, S Didehvar, S Farber, A Sacks, E Pirraglia, M J de Leon, A Bekker
Increasingly, postoperative cognitive dysfunction (POCD) is recognized as a complication after surgery in the elderly; but it's etiology remains unclear. Here we examine changes in cytokine levels during both the pre-operative and postoperative period, comparing them with long term variation in cognitive test scores. Forty-one patients aged 65 and older undergoing major surgery with general anesthesia were recruited after written consent in this IRB approved study. Thirty went on to complete this prospective, non-interven-tional and non-randomized study...
August 2016: International Journal of Anesthesiology & Research
https://www.readbyqxmd.com/read/28301313/erratum-proteomics-analysis-of-the-regulatory-role-of-rpf-dsf-cell-to-cell-signaling-system-in-the-virulence-of-xanthomonas-campestris
#11
(no author information available yet)
No abstract text is available yet for this article.
February 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28291554/implantation-of-posterior-chamber-phakic-intraocular-lens-for-myopia-and-hyperopia%C3%A2-%C3%A2-long-term-clinical-outcomes
#12
H Kocová, E Vlková, L Michalcová, O Motyka
PURPOSE: To evaluate long-term refractive outcomes of implantable collamer lens (ICL) implantation and late postoperative complications. METHODS: We assessed outcomes of patients who underwent ICL implantation (type ICM V4 for myopia, ICH V3 for hyperopia, TICM V4 for astigmatism) in our department between 1998 and 2013. It comprised 62 eyes (40 myopic and 22 hyperopic). The average follow-up period was 10.5 years. We evaluated: uncorrected and best-corrected visual acuity (UCVA and BCVA), spherical equivalent (SE), ICL vault, endothelial cell density and late postoperative complications...
March 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28258929/liver-transplantation-and-combined-hepatocellular-cholangiocarcinoma-feasibility-and-outcomes
#13
REVIEW
Paolo Magistri, Giuseppe Tarantino, Valentina Serra, Cristiano Guidetti, Roberto Ballarin, Fabrizio Di Benedetto
INTRODUCTION: Combined hepatocellular-cholangiocarcinoma (CHC or cHCC-CC) is a rare primary liver tumor displaying histological features of both hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). Most patients are not suitable for surgery because of the advanced stage of the disease at the moment of diagnosis. We decided to review the literature in order to identify the outcomes after liver transplantation for CHC and to clarify which is the most appropriate treatment. MATERIAL AND METHODS: A systematic literature search was performed...
February 6, 2017: Digestive and Liver Disease
https://www.readbyqxmd.com/read/28244030/characterization-of-a-bacillus-thuringiensis-chitinase-that-binds-to-cellulose-and-chitin
#14
Shotaro Honda, Toshiyuki Kunii, Kenta Nohara, Satoshi Wakita, Yasusato Sugahara, Masao Kawakita, Fumitaka Oyama, Masayoshi Sakaguchi
Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticidal process, chitinases are suggested to perforate the peritrophic membrane barrier to facilitate the invasion of the Cry proteins into epithelial membranes. A chitinase gene from B. thuringiensis was successfully expressed in a soluble form in Escherichia coli, and the gene product was purified and characterized. The purified recombinant enzyme, BthChi74, hydrolyzed an artificial substrate, 4-nitrophenyl N,N'-diacetyl-β-D-chitobioside [4NP-(GlcNAc)2], and the natural substrates, colloidal chitin and crystalline α-chitin, but it did not hydrolyze cellulose...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28243753/pain-in-parkinson-disease-a-cross-sectional-survey-of-its-prevalence-specifics-and-therapy
#15
Carsten Buhmann, Nathalie Wrobel, Wiebke Grashorn, Odette Fruendt, Katharina Wesemann, Sabrina Diedrich, Ulrike Bingel
We aimed to evaluate prevalence, phenotype, and therapeutic realities of pain in patients with Parkinson disease (PD). Therefore, we assessed 181 outpatients with PD using a cross-sectional approach applying the German Pain Questionaire (DSF), the PainDetect, and a self-developed Parkinson Disease Pain Questionaire (UPDPQ) covering detailed therapeutic aspects. Furthermore, we investigated the association between pain and PD-disease characteristics, quality of life (PDQ-39), depression, and anxiety (HADS-D, HADS-A)...
February 27, 2017: Journal of Neurology
https://www.readbyqxmd.com/read/28219041/coupling-the-advection-dispersion-equation-with-fully-kinetic-reversible-irreversible-sorption-terms-to-model-radiocesium-soil-profiles-in-fukushima-prefecture
#16
Hiroshi Kurikami, Alex Malins, Minoru Takeishi, Kimiaki Saito, Kazuki Iijima
Radiocesium is an important environmental contaminant in fallout from nuclear reactor accidents and atomic weapons testing. A modified Diffusion-Sorption-Fixation (mDSF) model, based on the advection-dispersion equation, is proposed to describe the vertical migration of radiocesium in soils following fallout. The model introduces kinetics for the reversible binding of radiocesium. We test the model by comparing its results to depth profiles measured in Fukushima Prefecture, Japan, since 2011. The results from the mDSF model are a better fit to the measurement data (as quantified by R(2)) than results from a simple diffusion model and the original DSF model...
May 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28151054/the-effect-of-organic-shock-loads-on-the-stability-of-anaerobic-granular-sludge
#17
Yangcheng Ding, Huajun Feng, Dongsheng Shen, Na Li, Meizhen Wang, Yuyang Zhou
This study investigated the effect of organic shock loads on the stability of anaerobic granular sludge (AnGS). At double and triple shock intensity (8 and 12 kg COD/m(3)d organic loading rate (OLR), respectively), the accumulation of volatile fatty acids resulted in the decreasing pH of the reactors. The unstable performances of AnGS also appeared in the macroscopic properties, including the decreasing contents of relative hydrophobicity (RH), granular strength and diameter, and the fluctuation of EPS production...
February 15, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28143565/disulfiram-overcomes-bortezomib-and-cytarabine-resistance-in-down-syndrome-associated-acute-myeloid-leukemia-cells
#18
Ranjan Bista, David W Lee, Oliver B Pepper, David O Azorsa, Robert J Arceci, Eiman Aleem
BACKGROUND: Children with Down syndrome (DS) have increased risk for developing AML (DS-AMKL), and they usually experience severe therapy-related toxicities compared to non DS-AMKL. Refractory/relapsed disease has very poor outcome, and patients would benefit from novel, less toxic, therapeutic strategies that overcome resistance. Relapse/resistance are linked to cancer stem cells with high aldehyde dehydrogenase (ALDH) activity. The purpose of the present work was to study less toxic alternative therapeutic agents for relapsed/refractory DS-AMKL...
February 1, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28126305/hypofractionated-regional-nodal-irradiation-for-women-with-node-positive-breast-cancer
#19
Sara Bellefqih, Sanaa Elmajjaoui, Jihan Aarab, Jihane Khalil, Mohamed Afif, Amine Lachgar, Hanan El Kacemi, Tayeb Kebdani, Noureddine Benjaafar
PURPOSE: To evaluate the effect of hypofractionated radiation therapy (HFRT) of the breast/chest wall and regional nodes on overall survival (OS), disease-free survival (DFS), locoregional control and on treatment-related toxicity in patients with breast cancer and nodal involvement. METHODS AND MATERIALS: Two hundred fifty-seven patients treated between October 2009 and June 2011 with hypofractionated locoregional radiation therapy (42 Gy in 15 fractions) were retrospectively reviewed, 51 (19...
March 1, 2017: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/28111334/the-selective-cytotoxicity-of-dsf-cu-attributes-to-the-biomechanical-properties-and-cytoskeleton-rearrangements-in-the-normal-and-cancerous-nasopharyngeal-epithelial-cells
#20
Yaping Yang, Mengjia Li, Xiaoxue Sun, Congran Zhou, Yawei Wang, Liwei Wang, Lixin Chen, Zhihong Liang, Linyan Zhu, Haifeng Yang
Cancer initiation and progression follow complex changes of cellular architecture and biomechanical property. Cancer cells with more submissive (or "softer") than their healthy counterparts attributed to the reorganization of the complex cytoskeleton structure, may be considered as a potential anti-tumor therapeutic target. In this study, atomic force microscopy (AFM) was carried out to detect the topographical and biophysical changes of nasopharyngeal carcinoma CNE-2Z cells and normal nasopharyngeal epithelial cells NP69-SV40T by treating the Disulfiram chelated with Cu(2+) (DSF-Cu)...
January 19, 2017: International Journal of Biochemistry & Cell Biology
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