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Non-ionic contrast

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https://www.readbyqxmd.com/read/27878040/effect-of-contrast-media-on-urinary-cytopathology-specimens
#1
Sebastian Frees, Samir Bidnur, Michael Metcalfe, Peter Raven, Claudia Chavez-Munoz, Igor Moskalev, Ladan Fazli, Alan So
INTRODUCTION: Urological dogma dictates that washings collected from the urinary tract for cytological assessment must be performed without interference from contrast agents that may alter cellular integrity and diagnostic interpretation. In practice, the initial contrast used to outline the upper tracts is commonly discarded with subsequent saline washings sent for cytology. We hypothesize that contrast washings do not affect the morphology of urothelial carcinoma cells or the integrity of cytology interpretation...
August 2016: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/27877155/weak-and-saturable-protein-surfactant-interactions-in-the-denaturation-of-apo-%C3%AE-lactalbumin-by-acidic-and-lactonic-sophorolipid
#2
Kell K Andersen, Brian S Vad, Sophie Roelants, Inge N A van Bogaert, Daniel E Otzen
Biosurfactants are of growing interest as sustainable alternatives to fossil-fuel-derived chemical surfactants, particularly for the detergent industry. To realize this potential, it is necessary to understand how they affect proteins which they may encounter in their applications. However, knowledge of such interactions is limited. Here, we present a study of the interactions between the model protein apo-α-lactalbumin (apo-aLA) and the biosurfactant sophorolipid (SL) produced by the yeast Starmerella bombicola...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27869282/adsorption-and-desorption-behavior-of-ionic-and-nonionic-surfactants-on-polymer-surfaces
#3
Giulia Magi Meconi, Nicholas Ballard, José M Asua, Ronen Zangi
We report combined experimental and computational studies aiming to elucidate the adsorption properties of ionic and nonionic surfactants on hydrophobic polymer surface such as poly(styrene). To represent these two types of surfactants, we choose sodium dodecyl sulfate and poly(ethylene glycol)-poly(ethylene) block copolymers, both commonly utilized in emulsion polymerization. By applying quartz crystal microbalance with dissipation monitoring we find that the non-ionic surfactants are desorbed from the poly(styrene) surface slower, and at low surfactant concentrations they adsorb with stronger energy, than the ionic surfactant...
November 21, 2016: Soft Matter
https://www.readbyqxmd.com/read/27868467/k-channel-reorganization-and-homeostatic-plasticity-during-postembryonic-development-biophysical-and-genetic-analyses-in-acutely-dissociated-drosophila-central-neurons
#4
Taixiang Saur, I-Feng Peng, Peng Jiang, Neng Gong, Wei-Dong Yao, Tian-Le Xu, Chun-Fang Wu
Intrinsic electric activities of neurons play important roles in establishing and refining neural circuits during development. However, how the underlying ionic currents undergo postembryonic reorganizations remains largely unknown. Using acutely dissociated neurons from larval, pupal, and adult Drosophila brains, we show drastic re-assemblies and compensatory regulations of voltage-gated (IKv) and Ca(2+)-activated (IK(Ca)) K(+ )currents during postembryonic development. Larval and adult neurons displayed prominent fast-inactivating IKv, mediated by the Shaker (Sh) channel to a large extent, while in the same neurons IK(Ca) was far smaller in amplitude...
November 21, 2016: Journal of Neurogenetics
https://www.readbyqxmd.com/read/27863354/contrast-media-use-in-patients-with-chronic-kidney-disease-undergoing-coronary-angiography-a-systematic-review-and-meta-analysis-of-randomized-trials
#5
Bhavi Pandya, Jean Chaloub, Valay Parikh, Sainath Gaddam, Jonathan Spagnola, Suzanne El-Sayegh, Marc Bogin, Ruben Kandov, James Lafferty, Sripal Bangalore
BACKGROUND: Patients with chronic kidney disease (CKD) undergoing coronary angiography (CA), adequate hydration and minimizing volume of contrast media (CM) are class 1b recommendations for preventing contrast induced nephropathy (CIN). Current data are insufficient to justify specific recommendations about isoosmolar vs. low-osmolar contrast media by the ACCF/AHA/SCAI guidelines. METHODS: Randomized trials comparing IOCM to LOCM in CKD stage 3 and above patients undergoing CA, and reporting incidence of CIN (defined by a rise in creatinine of 25% from baseline) were included in the analysis...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27826994/diagnostic-efficacy-of-single-pass-abdominal-multidetector-row-ct-prospective-evaluation-of-a-low-dose-protocol
#6
Luigi Camera, Immacolata Liccardo, Federica Romano, Raffaele Liuzzi, Antonio Rispo, Massimo Imbriaco, Anna Testa, Gaetano Luglio, Simona De Fronzo, Fabiana Castiglione, Luigi Bucci, Arturo Brunetti
OBJECTIVES: To evaluate the diagnostic efficacy of single-pass contrast-enhanced multi-detector CT (CE-MDCT) performed with a low radiation-high contrast (LR-HC) dose protocol in selected patients with non traumatic acute bowel disease. METHODS: Sixty-five (32 M; 33 F; aged 20-67 years) consecutive patients with non traumatic acute bowel disease underwent single-pass CE-MDCT performed 70-100 sec after i.v. bolus injection of a non ionic iodinated contrast media (370 mgI/ml)...
November 9, 2016: British Journal of Radiology
https://www.readbyqxmd.com/read/27809540/chemisorption-of-hydroxide-on-2d-materials-from-dft-calculations-graphene-versus-hexagonal-boron-nitride
#7
Benoit Grosjean, Clarisse Péan, Alessandro Siria, Lyderic Bocquet, Rodolphe Vuilleumier, Marie-Laure Bocquet
Recent nanofluidic measurements revealed strongly different surface charge mea- surements for boron-nitride and graphitic nanotubes when in contact with saline and alkaline water.1,2 These observations contrast with the similar reactivity of a graphene layer and its boron nitride counterpart, using Density Functional Theory (DFT) frame- work, for intact and dissociative adsorption of gaseous water molecules. Here, we in- vestigate by DFT in implicit water single and multiple adsorption of anionic hydroxide on single layers...
November 3, 2016: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/27796332/smooth-deuterated-cellulose-films-for-the-visualisation-of-adsorbed-bio-macromolecules
#8
Jielong Su, Vikram S Raghuwanshi, Warwick Raverty, Christopher J Garvey, Peter J Holden, Marie Gillon, Stephen A Holt, Rico Tabor, Warren Batchelor, Gil Garnier
Novel thin and smooth deuterated cellulose films were synthesised to visualize adsorbed bio-macromolecules using contrast variation neutron reflectivity (NR) measurements. Incorporation of varying degrees of deuteration into cellulose was achieved by growing Gluconacetobacter xylinus in deuterated glycerol as carbon source dissolved in growth media containing D2O. The derivative of deuterated cellulose was prepared by trimethylsilylation(TMS) in ionic liquid(1-butyl-3-methylimidazolium chloride). The TMS derivative was dissolved in toluene for thin film preparation by spin-coating...
October 31, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27792324/how-do-short-chain-non-ionic-detergents-destabilize-gpcrs
#9
Sangbae Lee, Allen Mao, Supriyo Bhattacharya, Nathan Robertson, Reinhard Grisshammer, Christopher G Tate, Nagarajan Vaidehi
Stability of detergent-solubilized GPCRs is crucial for their purification in a biologically relevant state and it is well-known that short chain detergents such as octylglucoside are more denaturing than long chain detergents such as dodecylmaltoside. However, the molecular basis for this phenomenon is poorly understood. To gain insights into the mechanism of detergent destabilization of GPCRs, we used atomistic molecular dynamics simulations of thermostabilized adenosine receptor (A2AR) mutants embedded in either a lipid bilayer or detergent micelles of alkylmaltosides and alkylglucosides...
October 28, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27789781/the-dnae-polymerase-from-deinococcus-radiodurans-features-reca-dependent-dna-polymerase-activity
#10
Lorenzo Randi, Alessandro Perrone, Mirko Maturi, Fabrizio Dal Piaz, Michela Camerani, Alejandro Hochkoeppler
We report here on the catalytic properties of the Deinococcus radiodurans DNA Polymerase III α subunit (αDr). The αDr enzyme was overexpressed in E. coli , both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA Polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inclusion bodies, αDr was devoid of E. coli RNA Polymerase, and was purified to homogeneity. When assayed with different DNA substrates, αDr featured slower DNA extension rates when compared to the corresponding enzyme from E...
October 27, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27785644/effect-of-ica-l-blockade-on-adrenergic-stimulation-in-developing-heart
#11
T L Zefirov, A M Kuptsova, R G Biktemirova, N I Ziyatdinova, A L Zefirov
The effect of verapamil-induced blockade of L-type calcium ionic currents (ICa,L) on the action of non-selective adrenergic cardiac stimulation by norepinephrine was examined during different periods of early postnatal ontogeny. In 1-week-old rats, intravenous norepinephrine induced a short-term tachycardia both with and without preliminary injected verapamil. In 3-week-old rats, norepinephrine alone produced no chronotropic effect; in contrast, it induced a biphasic tachycardia in verapamil-treated rats. In 6- and 20-month-old rats, norepinephrine induced a short-term tachycardia, which could be prevented by verapamil...
October 26, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27782655/nonlocal-electrostatics-in-ionic-liquids-the-key-to-an-understanding-of-the-screening-decay-length-and-screened-interactions
#12
Roland Kjellander
Screened electrostatic interactions in ionic liquids are investigated by means of exact statistical mechanical analysis combined with physical arguments that enhance the transparency and conceptual accessibility of the analysis and results. The constituent ions and immersed particles in the liquid can have arbitrary shapes and any internal charge distributions. The decay of the screened electrostatic potential and the free energy of interaction in ionic liquids can be exponentially damped oscillatory (like in molten simple salts) as well as plain exponential and long-ranged (like in dilute electrolyte solutions)...
September 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27767294/manipulating-and-visualizing-molecular-interactions-in-customized-nanoscale-spaces
#13
Gil Henkin, Daniel Berard, Francis Stabile, Marjan Shayegan, Jason S Leith, Sabrina R Leslie
We present a dynamically adjustable nanofluidic platform for formatting the conformations of and visualizing the interaction kinetics between biomolecules in solution, offering new time resolution and control of the reaction processes. This platform extends Convex Lens-induced Confinement (CLiC), a technique for imaging molecules under confinement, by introducing a system for in-situ modification of the chemical environment; this system uses a deep microchannel to diffusively exchange reagents within the nanoscale imaging region, whose height is fixed by a nanopost array...
October 21, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27763572/destabilization-mechanism-of-ionic-surfactant-on-curcumin-nanocrystal-against-electrolytes
#14
Heni Rachmawati, Annisa Rahma, Loaye Al Shaal, Rainer H Müller, Cornelia M Keck
We have successfully developed curcumin nanosuspension intended for oral delivery. The main purpose is to improve bioavailability through enhancing its solubility. The nanoparticles were stabilized using various stabilizers, including polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA), sodium carboxymethylcellulose (Na-CMC), d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), and sodium dodecyl sulfate (SDS). The average diameter of particles, microscopic appearance, and sedimentation of each preparation was observed and compared...
October 18, 2016: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/27762420/probing-driving-forces-in-aerolysin-and-%C3%AE-hemolysin-biological-nanopores-electrophoresis-versus-electroosmosis
#15
Mordjane Boukhet, Fabien Piguet, Hadjer Ouldali, Manuela Pastoriza-Gallego, Juan Pelta, Abdelghani Oukhaled
The transport of macromolecules through nanopores is involved in many biological functions and is today at the basis of promising technological applications. Nevertheless the interpretation of the dynamics of the macromolecule/nanopore interaction is still misunderstood and under debate. At the nanoscale, inside biomimetic channels under an external applied voltage, electrophoresis, which is the electric force acting on electrically charged molecules, and electroosmotic flow (EOF), which is the fluid transport associated with ions, contribute to the direction and magnitude of the molecular transport...
October 20, 2016: Nanoscale
https://www.readbyqxmd.com/read/27733735/physicochemical-evaluation-and-developability-assessment-of-co-amorphouses-of-low-soluble-drugs-and-comparison-to-the-co-crystals
#16
Katsuhiko Yamamoto, Taro Kojima, Masatoshi Karashima, Yukihiro Ikeda
To judge the developability and analyze functional mechanism of co-amorphouses, we investigated the physicochemical properties of co-amorphouses and compare the properties with the co-crystals having the same drug and counters. Co-amorphous compounds are a novel approach to improve the physicochemical properties of drugs. A co-amorphous is in an amorphous solid state allowing non-ionic interactions between drug molecules and counter molecules. The co-amorphous compounds composed of itraconazole (ITZ) with the organic carboxyl acid, fumaric acid (FA) or L-tartaric acid (TA), were prepared by mechanical grinding...
December 1, 2016: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27720960/pectin-non-starch-nanofibers-biocomposites-as-novel-gastrointestinal-resistant-prebiotics
#17
Alireza Chackoshian Khorasani, Seyed Abbas Shojaosadati
Incorporation of nanofibers of chitin (NC), lignocellulose (NLC) and bacterial cellulose (BNC) in pectin was studied to improve prebiotic activity and gastrointestinal resistance of the pectin-nanofibers biocomposites for protection of probiotics under simulated gastrointestinal conditions. The biocomposites were prepared using various compositions of pectin and nanofibers, which were designed using D-optimal mixture method. The incorporation of the nanofibers in pectin led to a slow degradation of the pectin-nanofibers biocomposites in contrast to their rapid swelling...
October 6, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27695562/postoperative-seizure-following-transforaminal-percutaneous-endoscopic-lumbar-discectomy
#18
Hayri Kertmen, Bora Gürer, Erdal Resit Yilmaz, Zeki Sekerci
Endoscopic surgery for lumbar disc herniation has been available for more than 30 years. Transforaminal percutaneous endoscopic lumbar discectomy is a well-known, safe, and effective method used for the treatment of the lumbar disc herniation. The published complications of the transforaminal percutaneous endoscopic lumbar discectomy consist of infections, thrombophlebitis, dysesthesia, dural tear, vascular injury, and death. Seizure after transforaminal percutaneous endoscopic lumbar discectomy is an extremely rare complication...
October 2016: Asian Journal of Neurosurgery
https://www.readbyqxmd.com/read/27679715/iohexol-plasma-clearance-for-measuring-glomerular-filtration-rate-in-clinical-practice-and-research-a-review-part-1-how-to-measure-glomerular-filtration-rate-with-iohexol
#19
Pierre Delanaye, Natalie Ebert, Toralf Melsom, Flavio Gaspari, Christophe Mariat, Etienne Cavalier, Jonas Björk, Anders Christensson, Ulf Nyman, Esteban Porrini, Giuseppe Remuzzi, Piero Ruggenenti, Elke Schaeffner, Inga Soveri, Gunnar Sterner, Bjørn Odvar Eriksen, Sten-Erik Bäck
While there is general agreement on the necessity to measure glomerular filtration rate (GFR) in many clinical situations, there is less agreement on the best method to achieve this purpose. As the gold standard method for GFR determination, urinary (or renal) clearance of inulin, fades into the background due to inconvenience and high cost, a diversity of filtration markers and protocols compete to replace it. In this review, we suggest that iohexol, a non-ionic contrast agent, is most suited to replace inulin as the marker of choice for GFR determination...
October 2016: Clinical Kidney Journal
https://www.readbyqxmd.com/read/27670442/contrast-associated-transient-cortical-blindness-three-cases-with-mri-and-electrophysiology-findings
#20
Marius Baguma, Nadia Younan, Frédéric London, Michel Ossemann, Yves Vandermeeren
Transient cortical blindness (TCB) is a rare but striking complication following contrast agent injection. TCB might be secondary to a direct toxicity of the contrast agent, leading to an osmotic disruption of the blood-brain barrier (BBB), with a preferential involvement of the posterior circulation and occipital cortex. We report a series of three patients with contrast medium-associated TCB (intra-arterial injection of non-ionic contrast agent during diagnostic cerebral angiography for two of them and coronary angioplasty for the other one)...
September 26, 2016: Acta Neurologica Belgica
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