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Vitamin D AND transcription factor

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https://www.readbyqxmd.com/read/29535889/effects-of-1-25-dihydroxyvitamin-d-3-on-the-differentiation-of-mc3t3-e1-osteoblast-like-cells
#1
Hyun-Soo Kim, Mingzhen Zheng, Do-Kyung Kim, Won-Pyo Lee, Sang-Joun Yu, Byung-Ock Kim
Purpose: The purpose of this study was to evaluate the effects of 1,25-dihydroxyvitamin D3 on the proliferation, differentiation, and matrix mineralization of MC3T3-E1 osteoblast-like cells in vitro. Methods: MC3T3-E1 osteoblastic cells and 1,25-dihydroxyvitamin D3 were prepared. Cytotoxic effects and osteogenic differentiation were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) activity assay, ALP staining, alizarin red S staining, and reverse transcription-polymerase chain reaction (RT-PCR) for osteogenic differentiation markers such as ALP, collagen type I (Col-I), osteocalcin (OCN), vitamin D receptor (VDR), and glyceraldehyde 3-phosphate dehydrogenase...
February 2018: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/29518658/in-vitro-profiling-of-toxic-effects-of-prominent-environmental-lower-chlorinated-pcb-congeners-linked-with-endocrine-disruption-and-tumor-promotion
#2
Kateřina Pěnčíková, Lucie Svržková, Simona Strapáčová, Jiří Neča, Iveta Bartoňková, Zdeněk Dvořák, Martina Hýžďalová, Jakub Pivnička, Lenka Pálková, Hans-Joachim Lehmler, Xueshu Li, Jan Vondráček, Miroslav Machala
The mechanisms contributing to toxic effects of airborne lower-chlorinated PCB congeners (LC-PCBs) remain poorly characterized. We evaluated in vitro toxicities of environmental LC-PCBs found in both indoor and outdoor air (PCB 4, 8, 11, 18, 28 and 31), and selected hydroxylated metabolites of PCB 8, 11 and 18, using reporter gene assays, as well as other functional cellular bioassays. We focused on processes linked with endocrine disruption, tumor promotion and/or regulation of transcription factors controlling metabolism of both endogenous compounds and xenobiotics...
March 5, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29476479/vitamin-d-3-supplementation-reduces-subsequent-brain-injury-and-inflammation-associated-with-ischemic-stroke
#3
Megan A Evans, Hyun Ah Kim, Yeong Hann Ling, Sandy Uong, Antony Vinh, T Michael De Silva, Thiruma V Arumugam, Andrew N Clarkson, Graeme R Zosky, Grant R Drummond, Brad R S Broughton, Christopher G Sobey
Acute inflammation can exacerbate brain injury after ischemic stroke. Beyond its well-characterized role in calcium metabolism, it is becoming increasingly appreciated that the active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25-VitD3 ), has potent immunomodulatory properties. Here, we aimed to determine whether 1,25-VitD3 supplementation could reduce subsequent brain injury and associated inflammation after ischemic stroke. Male C57Bl6 mice were randomly assigned to be administered either 1,25-VitD3 (100 ng/kg/day) or vehicle i...
February 23, 2018: Neuromolecular Medicine
https://www.readbyqxmd.com/read/29476020/vitamin-d-binding-protein-enhances-epithelial-ovarian-cancer-progression-by-regulating-the-insulin-like-growth-factor-1-akt-pathway-and-vitamin-d-receptor-transcription
#4
Yu-Fang Huang, Yi-Hui Wu, Wen-Fang Cheng, Shu-Ling Peng, Wan-Lin Shen, Cheng-Yang Chou
PURPOSE: Malignant ascites of epithelial ovarian cancer (EOC) help identify prognostic biomarkers or mechanisms of tumor progression. Vitamin D-binding protein (DBP) was revealed to be upregulated in EOC ascites in our previous proteomic study. Here, we examined the role of DBP in EOC. EXPERIMENTAL DESIGN: We analyzed ascites, serum, and tissue samples of patients with newly diagnosed EOC to determine the prognostic effects of DBP. We verified DBP function using orthotopic animal models and DBP regulation in ovarian cancer cell lines...
February 23, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29467625/effect-of-vitamin-d-in-hn9-10e-embryonic-hippocampal-cells-and-in-hippocampus-from-mptp-induced-parkinson-s-disease-mouse-model
#5
Samuela Cataldi, Cataldo Arcuri, Stéphane Hunot, Carmen Mecca, Michela Codini, Maria E Laurenti, Ivana Ferri, Elisabetta Loreti, Mercedes Garcia-Gil, Giovanna Traina, Carmela Conte, Francesco S Ambesi-Impiombato, Tommaso Beccari, Francesco Curcio, Elisabetta Albi
It has long been proven that neurogenesis continues in the adult brains of mammals in the dentatus gyrus of the hippocampus due to the presence of neural stem cells. Although a large number of studies have been carried out to highlight the localization of vitamin D receptor in hippocampus, the expression of vitamin D receptor in neurogenic dentatus gyrus of hippocampus in Parkinson's disease (PD) and the molecular mechanisms triggered by vitamin D underlying the production of differentiated neurons from embryonic cells remain unknown...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29444238/vitamin-d-receptor-restricts-th2-biased-inflammation-in-the-heart
#6
Jiangping Song, Xiao Chen, Liang Cheng, Man Rao, Kai Chen, Ningning Zhang, Jian Meng, Mengmeng Li, Zhi-Qiang Liu, Ping-Chang Yang
Background and aims: The aberrant immune responses play a critical role in the pathogenesis of myocarditis. Vitamin D receptor (VDR) has immune regulatory functions. This study aims to investigate the role of VDR in the restricting the immune inflammation in the heart. Methods: The human heart samples were obtained from the heart transplantation. T helper (Th)2 and Th1 responses in the heart tissue were characterized by histology and immune assay. VDR-/- mice and Rag2-/- mice were used in the experiments to test the role of VDR in the maintaining the homeostasis in the heart...
February 12, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29401702/bioactive-dietary-vdr-ligands-regulate-genes-encoding-biomarkers-of-skin-repair-that-are-associated-with-risk-for-psoriasis
#7
Amitis Karrys, Islam Rady, Roxane-Cherille N Chamcheu, Marya S Sabir, Sanchita Mallick, Jean Christopher Chamcheu, Peter W Jurutka, Mark R Haussler, G Kerr Whitfield
Treatment with 1,25-dihydroxyvitamin D₃ (1,25D) improves psoriasis symptoms, possibly by inducing the expression of late cornified envelope (LCE)3 genes involved in skin repair. In psoriasis patients, the majority of whom harbor genomic deletion of LCE3B and LCE3C (LCE3C_LCE3B-del), we propose that certain dietary analogues of 1,25D activate the expression of residual LCE3A/LCE3D/LCE3E genes to compensate for the loss of LCE3B/LCE3C in the deletant genotype. Herein, human keratinocytes (HEKn) homozygous for LCE3C_LCE3B-del were treated with docosahexaenoic acid (DHA) and curcumin, two low-affinity, nutrient ligands for the vitamin D receptor (VDR)...
February 4, 2018: Nutrients
https://www.readbyqxmd.com/read/29401596/prostaglandin-e-2-suppresses-hcap18-ll-37-expression-in-human-macrophages-via-ep2-ep4-implications-for-treatment-of-mycobacterium-tuberculosis-infection
#8
Min Wan, Xiao Tang, Rokeya Sultana Rekha, S S V Jagadeeswara Rao Muvva, Susanna Brighenti, Birgitta Agerberth, Jesper Z Haeggström
Prostaglandin (PG)E2 is an arachidonic acid-derived lipid mediator that plays an important role in inflammation and immunity. In this study, we demonstrate that PGE2 suppresses basal and 1,25-dihydroxy vitamin D3 (VD3 )-induced expression of hCAP18/LL-37 via E prostanoid (EP)2 and EP4 receptors. In humans, VD3 up-regulates vitamin D receptor (VDR) expression and promotes transcription of the cathelicidin hCAP18/LL-37 gene, whereas PGE2 counteracts this effect. We find that PGE2 induces the cAMP/PKA-signaling pathway and enhances the expression of the inhibitory transcription factor cAMP-responsive modulator/inducible cAMP early repressor, which prevents VDR expression and induction of hCAP18/LL-37 in human macrophages...
January 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29391005/parents-knowledge-attitudes-and-beliefs-regarding-sun-protection-in-children-a-qualitative-study
#9
Zoe Littlewood, Sheila Greenfield
BACKGROUND: Childhood is a critical period for sun protection, when the skin is particularly susceptible to the carcinogenic effects of ultraviolet radiation. Children are dependent upon parents to implement sun protective measures. Existing qualitative research exploring parents' attitudes and beliefs underpinning children's sun protection is from Australia, which has the highest melanoma incidence rates globally, and thus benefits from widespread sun protection awareness campaigns. Parents' sun protective behaviour may, therefore, differ between Australia and the UK...
February 1, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29386352/stop-codon-readthrough-generates-a-c-terminally-extended-variant-of-the-human-vitamin-d-receptor-with-reduced-calcitriol-response
#10
Gary Loughran, Irwin Jungreis, Ioanna Tzani, Michael Power, Ruslan I Dmitriev, Ivaylo P Ivanov, Manolis Kellis, John F Atkins
Although stop codon readthrough is used extensively by viruses to expand their gene expression, verified instances of mammalian readthrough have only recently been uncovered by systems biology and comparative genomics approaches. Previously our analysis of conserved protein coding signatures that extend beyond annotated stop codons predicted stop codon readthrough of several mammalian genes, all of which have been validated experimentally. Four mRNAs display highly efficient stop codon readthrough, and these mRNAs have a UGA stop codon immediately followed by CUAG (UGA_CUAG) that is conserved throughout vertebrates...
January 31, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29383703/epigenetic-changes-and-their-implications-in-autoimmune-hepatitis
#11
REVIEW
Albert J Czaja
BACKGROUND: The genetic risk of autoimmune hepatitis is insufficient to explain the observed risk, and epigenetic changes may explain disparities in disease occurrence in different populations within and between countries. The goal of this review is to examine how epigenetic changes induced by the environment or inherited as a phenotypic trait may affect autoimmune hepatitis and be amenable to therapeutic intervention. MATERIALS AND METHODS: Pertinent abstracts were identified in PubMed by multiple search terms...
January 31, 2018: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29349779/skeletogenesis-in-the-persian-sturgeon-acipenser-persicus-and-its-correlation-with-gene-expression-of-vitamin-k-dependent-proteins-during-larval-development
#12
H Eshaghzadeh, A Akbarzadeh, M Yarmohammadi, E Gisbert
The present study describes morphological development of the skeleton in the Persian sturgeon Acipenser persicus and discusses the hypothesis that expression of genes encoding vitamin K-dependent proteins (VKDP) might be correlated with the mineralization of skeletal tissues during early development in sturgeons. Results showed that development of cartilage started just after hatching (mean ± S.D., 10·9 ± 0·7 mm in total length, LT ) in the head and notochord, whereas the first signs of mineralization occurred in the dentary and in the dermopalatine and palatopterygoid elements of the upper jaw, coinciding with the onset of exogenous feeding (20·1 ± 1·5 mm LT )...
February 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29346788/vitamin-d-classic-and-novel-actions
#13
Ángel Gil, Julio Plaza-Diaz, María Dolores Mesa
BACKGROUND: Classically, vitamin D has been implicated in bone health by promoting calcium absorption in the gut and maintenance of serum calcium and phosphate concentrations, as well as by its action on bone growth and reorganization through the action of osteoblasts and osteoclasts cells. However, in the last 2 decades, novel actions of vitamin D have been discovered. The present report summarizes both classic and novel actions of vitamin D. SUMMARY: 1,25(OH)2 vitamin D, the active metabolite of vitamin D, also known as calcitriol, regulates not only calcium and phosphate homeostasis but also cell proliferation and differentiation, and has a key a role to play in the responses of the immune and nervous systems...
2018: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/29250167/associations-between-abnormal-vitamin-d-metabolism-pathway-function-and-non-small-cell-lung-cancer
#14
Nan Ge, Xiu-Mei Chu, Yun-Peng Xuan, Dun-Qiang Ren, Yongjie Wang, Kai Ma, Hui-Jiang Gao, Wen-Jie Jiao
Lung cancer is a type of malignant tumor derived from the respiratory system, which is the leading cause of cancer-associated mortality worldwide, of which ~80% of cases are attributable to non-small cell lung cancer (NSCLC). A previous study demonstrated that 1α,25-Dihydroxyvitamin D3 (1α,25(OH)2 D3 ), derived from the vitamin D metabolic pathway contributes an antitumor effect. Aberrant expression of the essential enzyme encoding genes, Cytochrome P450 Family 27 Subfamily A Member 1 ( CYP27A1 ), Cytochrome P450 Family 27 Subfamily B Member 1 ( CYP27B1 ), and Cytochrome P450 Family 24 Subfamily A Member 1 ( CYP24A1 ) may be associated with lung cancer...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29243793/a-new-antioxidant-formulation-reduces-the-apoptotic-and-damaging-effect-of-cigarette-smoke-extract-on-human-bronchial-epithelial-cells
#15
L Vanella, G Li Volti, A Distefano, M Raffaele, V Zingales, R Avola, D Tibullo, I Barbagallo
OBJECTIVE: In this study we evaluated the possible protective effect of an antioxidant formulation containing microfiltered milk derived polypeptides, Curcumin, Vitamin B2, Carnitine and N-Acetyl-cysteine (NAC) in an in vitro model of chronic obstructive pulmonary disease (COPD). MATERIALS AND METHODS: Human bronchial epithelial cells (16HBE) were used in this study. Cells were treated for 24 h in the presence or absence of 10% of cigarette smoke extract (CSE) and in the presence or absence of antioxidant formulation...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29218094/mir-351-negatively-regulates-osteoblast-differentiation-of-mscs-induced-by-cholesten-3-one-through-targeting-vdr
#16
Qiuke Hou, Yongquan Huang, Yiwen Luo, Bin Wang, Yamei Liu, Rudong Deng, Saixia Zhang, Fengbin Liu, Dongfeng Chen
Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. Overexpression of miR-351 by miR-351 precursor transfection markedly inhibited the expression of osteoblast-specific genes, such as alkaline phosphatase (ALP), collagen type II, osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), which consequently decreased a number of calcium mineralized nodules...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29182680/differential-activity-of-2-methylene-19-nor-vitamin-d-analogs-on-growth-factor-gene-expression-in-rhino-mouse-skin-and-comparison-to-all-trans-retinoic-acid
#17
COMPARATIVE STUDY
Jamie M Ahrens, James D Jones, Nirca J Nieves, Ann M Mitzey, Hector F DeLuca, Margaret Clagett-Dame
While all 2-methylene-19-nor analogs of 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) tested produce an increase in epidermal thickness in the rhino mouse, only a subset reduce utricle size (comedolysis). All-trans retinoic acid (atRA) also causes epidermal thickening and a reduction in utricle size in the rhino mouse. We now report that 2-methylene-19-nor-(20S)-1α-hydroxybishomopregnacalciferol (2MbisP), a comedolytic analog, increases epidermal thickening more rapidly than does atRA, while both reduce utricle area at an equal rate...
2017: PloS One
https://www.readbyqxmd.com/read/29178579/vitamin-d-receptor-expression-in-canine-mammary-gland-and-relationship-with-clinicopathological-parameters-and-progesterone-oestrogen-receptors
#18
R Sánchez-Céspedes, M D Fernández-Martínez, A Raya, C Pineda, I López, Y Millán
The vitamin D receptor (VDR) belongs to the nuclear class II receptor family. VDR is a ligand transcription factor and mediates the actions of calcitriol, the active product of vitamin D synthesis. Nowadays, it is known that the biological actions of calcitriol include the capacity to modulate cancer features, such as proliferation and differentiation, apoptosis, angiogenesis, invasion and metastasis. VDR expression has been demonstrated in human breast cancer and vitamin D has emerged as a promising targeted therapy...
March 2018: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/29136005/a-17-molecule-set-as-a-predictor-of-complete-response-to-neoadjuvant-chemotherapy-with-docetaxel-cisplatin-and-5-fluorouracil-in-esophageal-cancer
#19
Hajime Fujishima, Shoichi Fumoto, Tomotaka Shibata, Kohei Nishiki, Yoshiyuki Tsukamoto, Tsuyoshi Etoh, Masatsugu Moriyama, Norio Shiraishi, Masafumi Inomata
BACKGROUND: Recently, neoadjuvant chemotherapy with docetaxel/cisplatin/5-fluorouracil (NAC-DCF) was identified as a novel strong regimen with a high rate of pathological complete response (pCR) in advanced esophageal cancer in Japan. Predicting pCR will contribute to the therapeutic strategy and the prevention of surgical invasion. However, a predictor of pCR after NAC-DCF has not yet been developed. The aim of this study was to identify a novel predictor of pCR in locally advanced esophageal cancer treated with NAC-DCF...
2017: PloS One
https://www.readbyqxmd.com/read/29115927/transcriptomic-responses-to-wounding-meta-analysis-of-gene-expression-microarray-data
#20
Piotr Andrzej Sass, Michał Dąbrowski, Agata Charzyńska, Paweł Sachadyn
BACKGROUND: A vast amount of microarray data on transcriptomic response to injury has been collected so far. We designed the analysis in order to identify the genes displaying significant changes in expression after wounding in different organisms and tissues. This meta-analysis is the first study to compare gene expression profiles in response to wounding in as different tissues as heart, liver, skin, bones, and spinal cord, and species, including rat, mouse and human. RESULTS: We collected available microarray transcriptomic profiles obtained from different tissue injury experiments and selected the genes showing a minimum twofold change in expression in response to wounding in prevailing number of experiments for each of five wound healing stages we distinguished: haemostasis & early inflammation, inflammation, early repair, late repair and remodelling...
November 7, 2017: BMC Genomics
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