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https://www.readbyqxmd.com/read/27646677/improving-icsi-a-review-from-the-spermatozoon-perspective
#1
Mara Simopoulou, Laertis Gkoles, Panagiotis Bakas, Polina Giannelou, Theodoros Kalampokas, Konstantinos Pantos, Michael Koutsilieris
: Intracytoplasmic sperm injection (ICSI) is the most frequently applied method for fertilization making the process of identifying the perfect spermatozoon fundamental. Herein we offer a critical and thorough presentation on the techniques reported regarding (i) handling and preparing semen samples, (ii) identifying and 'fishing' spermatozoa, and (iii) improving key factors, such as motility for a successful ICSI practice. These approaches are suggested to make the process easier and more effective especially in atypical and challenging circumstances...
December 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27075719/evidence-for-decreased-expression-of-appl1-associated-with-reduced-insulin-and-adiponectin-receptors-expression-in-pcos-patients
#2
R Dehghan, M Saidijam, M Mehdizade, N Shabab, M Yavangi, T Artimani
PURPOSE: To investigate the expression of Adaptor protein containing a PH domain, PTB domain and leucine zipper motif 1 (APPL1), insulin receptor (INSR), adiponectin and adiponectin receptors (adipoR1 and R2) and their possible associations in granulosa cells (GCs) of 22 polycystic ovary syndrome (PCOS) women compared to the 22 non-PCOS controls with normal ovulatory function matched for BMI (body mass index). METHODS: In this study, 44 infertile women aged 18-40 years undergoing in vitro fertilization (IVF) protocol were recruited...
September 2016: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/26161404/anesthesia-related-toxic-effects-on-in-vitro-fertilization-outcome-burden-of-proof
#3
REVIEW
Paraskevi Matsota, Eva Kaminioti, Georgia Kostopanagiotou
Management of pain and anxiety during oocyte retrieval makes anesthesia an important part of the in vitro fertilization (IVF) procedures. There are many studies investigating the influence of anesthesia on IVF success. This review article provides an overview of published data regarding the potential toxic effects of different anesthetic techniques (Loco-regional, general anesthesia (GA), and monitored anesthesia care (MAC)), different anesthetic agents, and alternative medicine approach (principally acupuncture) on the IVF outcome...
2015: BioMed Research International
https://www.readbyqxmd.com/read/25349438/biopsy-induced-inflammatory-conditions-improve-endometrial-receptivity-the-mechanism-of-action
#4
Y Gnainsky, I Granot, P Aldo, A Barash, Y Or, G Mor, N Dekel
A decade ago, we first reported that endometrial biopsy significantly improves the success of pregnancy in IVF patients with recurrent implantation failure, an observation that was later confirmed by others. Recently, we have demonstrated that this treatment elevated the levels of endometrial pro-inflammatory cytokines and increased the abundance of macrophages (Mac) and dendritic cells (DCs). We therefore hypothesised that the biopsy-related successful pregnancy is secondary to an inflammatory response, and aimed at deciphering its mechanism of action...
January 2015: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/25305728/removal-of-annexin-v-positive-sperm-cells-for-intracytoplasmic-sperm-injection-in-ovum-donation-cycles-does-not-improve-reproductive-outcome-a-controlled-and-randomized-trial-in-unselected-males
#5
RANDOMIZED CONTROLLED TRIAL
Laura Romany, Nicolás Garrido, Yamileth Motato, Belén Aparicio, José Remohí, Marcos Meseguer
OBJECTIVE: To determine the effect of removing presumptive apoptotic sperm cells from samples from unselected males by means of magnetic activated cell sorting (MACS) on live-birth delivery rates after intracytoplasmic sperm injection (ICSI) in couples undergoing ovum donation (OD). DESIGN: Prospective, randomized, triple-blinded, and controlled study. SETTING: Private university-affiliated IVF center. PATIENT(S): A total of 237 infertile couples undergoing ICSI as part of an OD program...
December 2014: Fertility and Sterility
https://www.readbyqxmd.com/read/24809430/the-role-of-inflammation-for-a-successful-implantation
#6
REVIEW
Nava Dekel, Yulia Gnainsky, Irit Granot, Karen Racicot, Gil Mor
Approximately half of all human embryo implantations result in failed pregnancy. Multiple factors may contribute to this failure, including genetic or metabolic abnormalities of the embryo. However, many of these spontaneous early abortion cases are attributed to poor uterine receptivity. Furthermore, although many fertility disorders have been overcome by a variety of assisted reproductive techniques, implantation remains the rate-limiting step for the success of the in vitro fertilization (IVF) treatments...
August 2014: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/24057155/case-report-the-use-of-annexin-v-coupled-with-magnetic-activated-cell-sorting-in-cryopreserved-spermatozoa-from-a-male-cancer-survivor-healthy-twin-newborns-after-two-previous-icsi-failures
#7
María Belén Herrero, Géraldine Delbes, Jin-Tae Chung, Weon-Young Son, Hananel Holzer, William Buckett, Peter Chan
PURPOSE: The aim of the study is to report successful outcome (live births) after sperm sorting with annexin V-MACS on cryopreserved spermatozoa with high level of sperm DNA fragmentation from a cancer patient survivor. METHODS: Cryopreserved spermatozoa were sorted with annexin V-MACS prior to ICSI. Sperm DNA fragmentation was evaluated by SCSA(®) and TUNEL. RESULTS: The couple had two previous IVF/ICSI cycles failures using sperm cryopreserved before cancer treatment...
November 2013: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/23203214/age-shock-misperceptions-of-the-impact-of-age-on-fertility-before-and-after-ivf-in-women-who-conceived-after-age-40
#8
K Mac Dougall, Y Beyene, R D Nachtigall
STUDY QUESTION: What do older women understand of the relationship between age and fertility prior and subsequent to delivering their first child? SUMMARY ANSWER: Women who were first-time parents over the age of 40 did not accurately perceive the relationship between age and fertility prior to conceiving with IVF. WHAT IS KNOWN ALREADY: While increases in women's age at their first birth have been most pronounced in relatively older women, the rapidity of fertility decline is not appreciated by most non-infertility specialist physicians, the general public or men and women who are delaying childbearing...
February 2013: Human Reproduction
https://www.readbyqxmd.com/read/22333985/-inconvenient-biology-advantages-and-disadvantages-of-first-time-parenting-after-age-40-using-in-vitro-fertilization
#9
K Mac Dougall, Y Beyene, R D Nachtigall
BACKGROUND: As ages at first birth have steadily risen in the industrial west over the last several decades, the phenomenon of 'delayed childbearing' has come under research scrutiny by demographers, medical specialists and social scientists. In this study, we specifically explore the perceived advantages and disadvantages of postponed conception as well as participants' retrospective opinions on the 'optimal age' for parenting. METHODS: To this end, we examined a cohort purposely chosen to epitomize delayed childbearing, i...
April 2012: Human Reproduction
https://www.readbyqxmd.com/read/20359706/what-do-patients-want-expectations-and-perceptions-of-ivf-clinic-information-and-support-regarding-frozen-embryo-disposition
#10
Robert D Nachtigall, Kirstin Mac Dougall, Matthew Lee, Jennifer Harrington, Gay Becker
OBJECTIVE: To describe frozen embryo holders' expectations and perceptions of IVF clinic information, support, and storage fees and their relevance to embryo disposition decision making. DESIGN: Qualitative interview study. SETTING: Three northern California IVF practices. PATIENT(S): One hundred six families (110 women and 74 men) with an average of six frozen embryos in storage for 5 years. INTERVENTION(S): Three in-depth interviews over 1 year...
November 2010: Fertility and Sterility
https://www.readbyqxmd.com/read/20150175/magnetic-activated-cell-sorting-for-sperm-preparation-reduces-spermatozoa-with-apoptotic-markers-and-improves-the-acrosome-reaction-in-couples-with-unexplained-infertility
#11
Tsung-Hsien Lee, Chung-Hsien Liu, Yang-Tse Shih, Hui-Mei Tsao, Chun-Chia Huang, Hsiu-Hui Chen, Maw-Sheng Lee
BACKGROUND: Couples with unexplained infertility (UI) tend to have low fertilization rates with current IVF procedures. Here, we attempted to identify spermatozoa with apoptotic markers in couples with UI and unsuccessful intrauterine insemination (IUI) and we investigated the efficiency and benefit of magnetic-activated cell sorting (MACS) for sperm preparation in such patients. METHODS: Sixty couples with UI and two IUI failures were recruited. The sperm were prepared by conventional density gradient centrifugation (DGC) and divided into two aliquots...
April 2010: Human Reproduction
https://www.readbyqxmd.com/read/19700150/how-couples-who-have-undergone-in-vitro-fertilization-decide-what-to-do-with-surplus-frozen-embryos
#12
Robert D Nachtigall, Kirstin Mac Dougall, Jennifer Harrington, Julia Duff, Matthew Lee, Gay Becker
In a qualitative interview study of 77 families with stored frozen embryos, we found that while embryo disposition decision making was influenced by individual life circumstances, embryo quantity/quality, personal values, embryo conceptualization, and clinic information, it was a stepwise process that could be represented as three sequential questions: (1) Will the embryos be used for additional attempts at conception? If not, (2) Will the embryos remain in storage? And if not, (3) Will the embryos be donated to other people or to science, or will they be destroyed? While almost two-thirds (63%) of participants kept their embryos in storage after 5 years, either passively through disagreement or indecision or actively to maintain embryo potential, avert feelings of loss, or as psychological or genetic "insurance," IVF clinic support and detailed information about options motivated families to make disposition decisions...
December 2009: Fertility and Sterility
https://www.readbyqxmd.com/read/17337472/molecular-glass-wool-filtration-as-a-new-tool-for-sperm-preparation
#13
Sonja Grunewald, Werner Miska, Gisela Miska, Manja Rasch, Martin Reinhardt, Hans-Juergen Glander, Uwe Paasch
BACKGROUND: Magnetic-activated cell sorting (MACS) using annexin V-conjugated microbeads in a liquid phase eliminates apoptotic spermatozoa based on the externalization of phosphatidylserine (EPS) residues. The procedure allows the enrichment of a sperm population free of apoptosis markers, giving higher fertilization potential. Our aim was to determine if the annexin V binding principle can be transferred onto a glass wool filter system in order to produce a solid phase filter. METHODS: Semen samples (n = 42) were subjected to a molecular glass wool filter system using glass surfaces coated with annexin V and compared with aliquots separated by conventional glass wool, as well as with annexin V-MACS...
May 2007: Human Reproduction
https://www.readbyqxmd.com/read/12654017/advances-in-biotechnology-new-tools-in-future-pig-production-for-agriculture-and-biomedicine
#14
REVIEW
H Niemann, D Rath, C Wrenzycki
Biotechnology in livestock comprises an arsenal of reproductive biotechniques and molecular genetics. While molecular genetics are poorly developed in swine, reproductive techniques are more advanced and applied under field conditions. This review describes three selected examples of our own research to illustrate the implication of biotechnology in future pig reproduction. Sperm sexing technology is now available and can be used to generate piglets of the desired sex by IVF and ICSI. First studies also indicate satisfactory success rates following intrauterine insemination with sexed spermatozoa...
April 2003: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/11880013/general-anesthesia-versus-monitored-anesthesia-care-with-remifentanil-for-assisted-reproductive-technologies-effect-on-pregnancy-rate
#15
COMPARATIVE STUDY
Wolfram Wilhelm, Mohamad E Hammadeh, Paul F White, Thomas Georg, Rosine Fleser, Andreas Biedler
STUDY OBJECTIVES: To compare the outcome of assisted reproductive technology procedures in women who undergo monitored anesthesia care (MAC) with remifentanil versus general anesthesia. DESIGN: Retrospective data analysis. SETTING: University hospital. PATIENTS: 251 ASA physical status I and II women participating in an in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) program. INTERVENTIONS: During the first phase of the study, all patients underwent general anesthesia induction with alfentanil, propofol, and nitrous oxide, which was maintained with isoflurane or propofol infusion...
February 2002: Journal of Clinical Anesthesia
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