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https://www.readbyqxmd.com/read/28643946/activated-proline-rich-tyrosine-kinase-2-regulates-meiotic-spindle-assembly-in-the-mouse-oocyte
#1
Xiao-Qian Meng, Bing Cui, Dong Cheng, Hui Lyu, Li-Gang Jiang, Ke-Gang Zheng, Shu-Zhen Liu, Jie Pan, Cong Zhang, Jing Bai, Jun Zhou
Proline-rich tyrosine kinase 2 (PYK2), a member of the protein tyrosine kinase family, plays an important role in various cellular processes. PYK2 can be phosphorylated on tyrosine 402 by diverse stimuli at the cell surface, and recent studies have shown that this activated form of PYK2 is enriched in oocytes and required for fertilization. However, the subcellular localization and functions of activated PYK2 in oocytes remain elusive. In this study, we demonstrate that the localization of p-PYK2 undergoes dynamic changes during in vitro maturation of mouse oocytes...
June 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28634819/establishment-of-a-tagged-variant-of-lgr4-receptor-suitable-for-functional-and-expression-studies-in-the-mouse
#2
Vitezslav Kriz, Michaela Krausova, Petra Buresova, Jan Dobes, Dusan Hrckulak, Olga Babosova, Jiri Svec, Vladimir Korinek
Leucine-rich repeat-containing G-protein-coupled receptor 4 (LGR4) is produced in a broad spectrum of mouse embryonic and adult tissues and its deficiency results in embryonal or perinatal lethality. The LGR4 function was mainly related to its potentiation of canonical Wnt signaling; however, several recent studies associate LGR4 with additional signaling pathways. To obtain a suitable tool for studying the signaling properties of Lgr4, we generated a tagged variant of the Lgr4 receptor using gene targeting in the mouse oocyte...
June 20, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28615648/embryonic-exposure-to-the-widely-used-herbicide-atrazine-disrupts-meiosis-and-normal-follicle-formation-in-female-mice
#3
Aurore Gely-Pernot, Souhila Saci, Pierre-Yves Kernanec, Chunxiang Hao, Frank Giton, Christine Kervarrec, Sergei Tevosian, Severine Mazaud-Guittot, Fatima Smagulova
The widely-used herbicide atrazine (ATZ) is detected in ground and surface water in many countries. Several studies in animals have demonstrated that ATZ has endocrine-disrupting effects on male and female reproduction in many vertebrate species. In this study, we investigated the effects of ATZ exposure on meiosis, a key step in gametogenesis in mammals. The treatment was initiated before oocyte entry into meiosis, which occurs during the embryonic period in females. We found that embryonic exposure to ATZ increases the level of 8-oxo-guanine in the nucleus of meiotic cells, reflecting oxidative stress and affecting meiotic double-strand break repair, chromosome synapsis and crossover numbers...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28603497/sigma-1-receptor-plays-a-negative-modulation-on-n-type-calcium-channel
#4
Kang Zhang, Zhe Zhao, Liting Lan, Xiaoli Wei, Liyun Wang, Xiaoyan Liu, Haitao Yan, Jianquan Zheng
The sigma-1 receptor is a 223 amino acids molecular chaperone with a single transmembrane domain. It is resident to eukaryotic mitochondrial-associated endoplasmic reticulum and plasma membranes. By chaperone-mediated interactions with ion channels, G-protein coupled receptors and cell-signaling molecules, the sigma-1 receptor performs broad physiological and pharmacological functions. Despite sigma-1 receptors have been confirmed to regulate various types of ion channels, the relationship between the sigma-1 receptor and N-type Ca(2+) channel is still unclear...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28595033/temperature-gradients-in-vivo-influence-maturing-male-and-female-gametes-in-mammals-evidence-from-the-cow
#5
R H F Hunter, F López-Gatius, O López-Albors
Since 1980 several reports have indicated that temperatures vary between preovulatory follicles and other ovarian tissues in rabbit, cow, pig and human. However, these observations did not achieve prominence; they were regarded as artefacts due to the use of anaesthetics and open surgery (laparotomy). Recently, without resorting to anaesthesia or surgery, direct measurements of temperature in preovulatory follicles have been performed in the cow by means of a thermistor probe introduced into the antrum under ultrasonic guidance...
June 9, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28552396/the-effect-of-histone-acetyl-transferase-inhibitor-trichostatin-a-on-porcine-mesenchymal-stem-cell-transcriptome
#6
Artur Gurgul, Jolanta Opiela, Klaudia Pawlina, Tomasz Szmatoła, Michał Bochenek, Monika Bugno-Poniewierska
The use of histone acetyl-transferase inhibitors such as trichostatin A (TSA) for epigenetic transformation of mesenchymal stem cells (MSCs), whose nuclei will be transferred into enucleated oocytes, is a novel approach in research involving somatic cell cloning of pigs and other mammalian species. Although the effectiveness of TSA in cloning applications was confirmed, processes and mechanisms underlying achieved effects are not yet fully understood, especially for pig MSCs. To contribute to this knowledge, in this study we performed a comprehensive transcriptome analysis using high-throughput sequencing of pig bone-marrow derived MSCs, both treated and untreated with TSA, and evaluated the effect of TSA administration on their transcription profile after 24 h of in vitro culture...
May 25, 2017: Biochimie
https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#7
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28448636/successful-vitrification-of-pronuclear-stage-pig-embryos-with-a-novel-cryoprotective-agent-carboxylated-%C3%AE%C2%B5-poly-l-lysine
#8
Maki Kamoshita, Tsubasa Kato, Katsuyoshi Fujiwara, Takafumi Namiki, Kazuaki Matsumura, Suong-Hyu Hyon, Junya Ito, Naomi Kashiwazaki
Vitrification is a powerful tool for the efficient production of offspring derived from cryopreserved oocytes or embryos in mammalian species including domestic animals. Genome editing technologies such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas)9 are now available even for domestic species, suggesting that the vitrification of embryos at the pronuclear stage (PN) will be more important because they could provide genomic host cells to be targeted by TALENs or CRISPR/Cas9...
2017: PloS One
https://www.readbyqxmd.com/read/28442066/characterisation-and-cryopreservation-of-the-ovarian-preantral-follicle-population-from-spix-s-yellow-toothed-cavies-galea-spixii-wagler-1831
#9
Érica C G Praxedes, Gabriela L Lima, Andréia M Silva, Carlos A C Apolinário, José A B Bezerra, Ana L P Souza, Moacir F Oliveira, Ana P R Rodrigues, Alexandre R Silva
The aim of the present study was to characterise the ovarian preantral follicle (PF) population and to establish a solid surface vitrification (SSV) process using dimethyl sulfoxide (DMSO) as a cryoprotectant for preservation of ovarian tissue from yellow-toothed cavies (Galea spixii). Ovaries were fixed for PF population analysis or were subjected to the SSV process. The mean (± s.e.m.) PF population per ovarian pair was estimated to be 416.0±342.8. There were 140.0±56.0 (63.4%) and 125.0±58.0 (64.0%) primary follicles on the right and left ovaries, respectively...
March 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28433565/inhibitory-effects-of-pine-nodule-extract-and-its-component-sj-2-on-acetylcholine-induced-catecholamine-secretion-and-synthesis-in-bovine-adrenal-medullary-cells
#10
Xiaojia Li, Takafumi Horishita, Yumiko Toyohira, Hui Shao, Jie Bai, Haixia Bo, Xinbo Song, Shin Ishikane, Yukari Yoshinaga, Noriaki Satoh, Masato Tsutsui, Nobuyuki Yanagihara
Extract of pine nodules (matsufushi) formed by bark proliferation on the surface of trees of Pinus tabulaeformis or Pinus massoniana has been used as an analgesic for joint pain, rheumatism, neuralgia, dysmenorrhea and other complaints in Chinese traditional medicine. Here we report the effects of matsufushi extract and its components on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. We found that matsufushi extract (0.0003-0.005%) and its component, SJ-2 (5-hydroxy-3-methoxy-trans-stilbene) (0...
April 2017: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/28397763/endoplasmic-reticulum-stress-and-homeostasis-in-reproductive-physiology-and-pathology
#11
REVIEW
Elif Guzel, Sefa Arlier, Ozlem Guzeloglu-Kayisli, Mehmet Selcuk Tabak, Tugba Ekiz, Nihan Semerci, Kellie Larsen, Frederick Schatz, Charles Joseph Lockwood, Umit Ali Kayisli
The endoplasmic reticulum (ER), comprises 60% of the total cell membrane and interacts directly or indirectly with several cell organelles i.e., Golgi bodies, mitochondria and proteasomes. The ER is usually associated with large numbers of attached ribosomes. During evolution, ER developed as the specific cellular site of synthesis, folding, modification and trafficking of secretory and cell-surface proteins. The ER is also the major intracellular calcium storage compartment that maintains cellular calcium homeostasis...
April 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28392504/defects-in-the-first-wave-of-folliculogenesis-in-mouse-xo-ovaries
#12
Kento Miura, Chiharu Murata, Kyoko Harikae, Hitomi Suzuki, Masami Kanai-Azuma, Masamichi Kurohmaru, Naoki Tsunekawa, Yoshiakira Kanai
In mouse ovaries, the first wave of folliculogenesis perinatally starts near the medullary region, which directs the initial round of follicular growth soon after birth. At the same time, cortical primordial follicles start forming in the ovarian surface region, and then some are cyclically recruited for the second and subsequent rounds of follicular growth. Recent studies suggest different dynamics between the first and subsequent waves of follicular growth in postnatal ovaries. However, the phenotypic differences between these phases remain unclear...
June 21, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28366629/affinity-chromatography-of-a-binder-of-1-methyladenine-the-maturation-inducing-hormone-for-starfish-oocytes
#13
Tetsuo Toraya, Atsushi Kuyama, Seiichi Tanaka, Masatoyo Yamamoto, Tadamasa Ohmiya, Yuri Saito, Tomoko Tanabe
Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary. They resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the maturation-inducing hormone for starfish oocytes. Putative 1-MeAde receptors have been suggested to be present on the oocyte surface, but not yet been characterized biochemically. As reported recently (T. Toraya, T. Kida, A. Kuyama, S. Matsuda, S. Tanaka, Y. Komatsu, T. Tsurukai, Biochem. Biophys. Res. Commun. 485 (2017) 41-46), it became possible to detect unknown 1-MeAde binders of starfish oocytes by immunophotoaffinity labeling, i...
May 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28343714/sensitivity-of-eastern-oyster-crassostrea-virginica-spermatozoa-and-oocytes-to-dispersed-oil-cellular-responses-and-impacts-on-fertilization-and-embryogenesis
#14
J Vignier, A K Volety, A Rolton, N Le Goïc, F-L E Chu, R Robert, P Soudant
The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF...
March 23, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28285997/ccm-3-promotes-c-%C3%A2-elegans-germline-development-by-regulating-vesicle-trafficking-cytokinesis-and-polarity
#15
Swati Pal, Benjamin Lant, Bin Yu, Ruilin Tian, Jiefei Tong, Jonathan R Krieger, Michael F Moran, Anne-Claude Gingras, W Brent Derry
Cerebral cavernous malformations (CCMs) are vascular defects of the CNS that arise from loss of integrity of the endothelial cells lining blood capillaries, causing leakage of blood into the brain [1]. This results in headaches, seizures, and/or hemorrhagic stroke, depending on the location of the lesion. CCM affects 0.5% of the population and follows an autosomal dominant inheritance pattern caused by mutations in one of the three genes: CCM1 (gene name KRIT1), CCM2 (also known as malcavernin or OSM), and CCM3 (gene name PDCD10) [2, 3], with the earliest onset and most severe prognosis occurring in CCM3 patients [4]...
March 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28248633/evaluation-of-smartflare-probe-applicability-for-verification-of-rnas-in-early-equine-conceptuses-equine-dermal-fibroblast-cells-and-trophoblastic-vesicles
#16
S Budik, W Tschulenk, S Kummer, I Walter, C Aurich
Live cell RNA imaging has become an important tool for studying RNA localisation, dynamics and regulation in cultured cells. Limited information is available using these methods in more complex biological systems, such as conceptuses at different developmental stages. So far most of the approaches rely on microinjection of synthetic constructs into oocytes during or before fertilisation. Recently, a new generation of RNA-specific probes has been developed, the so named SmartFlare probes (Merck Millipore). These consist of a central 15-nm gold particle with target-specific DNAs immobilised on its surface...
March 2, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28238840/study-of-the-effect-of-ulipristal-acetate-on-human-sperm-ability-to-interact-with-tubal-tissue-and-cumulus-oocyte-complexes
#17
Carlos Zumoffen, Matías D Gómez-Elías, Adriana M Caille, Luis Bahamondes, Patricia S Cuasnicú, Débora J Cohen, María José Munuce
OBJECTIVE: Ulispristal acetate (UPA) is a selective progesterone receptor modulator widely used for emergency contraception (EC). The described main mechanism of action is by inhibiting or delaying ovulation; however, the postovulatory effects of the drug are still on debate. Therefore, the aim of this study was to determine whether UPA could interfere with human sperm fertilizing ability. STUDY DESIGN: Human motile spermatozoa were incubated under capacitating conditions with or without UPA, and then used to inseminate human tubal explants, mouse cumulus-oocyte complexes and zona-free hamster eggs...
June 2017: Contraception
https://www.readbyqxmd.com/read/28238709/occludin-expression-and-regulation-in-small-follicles-of-the-layer-and-broiler-breeder-hen
#18
C S Stephens, P A Johnson
Synchronized yolk accumulation and follicle development are essential for egg production in oviparous species. In birds, yolk is incorporated into the oocyte by an avian specific yolk receptor (LR8), and it has been suggested that occludin (OCLN), a tight junction protein, mediates transfer of yolk material to the oocyte surface. OCLN may be a key regulator of yolk accumulation and follicle growth, however, the expression and regulation of OCLN in granulosa cells during various stages of follicle development is unknown...
February 24, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28202471/oocyte-shuttle-a-recombinant-protein-transporting-donor-dna-into-the-xenopus-oocyte-in-situ
#19
Duri Rungger, Lisbeth Muster, Oleg Georgiev, Elisabeth Rungger-Brändle
The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site...
February 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28174006/immunophotoaffinity-labeling-of-binders-of-1-methyladenine-the-oocyte-maturation-inducing-hormone-of-starfish
#20
Tetsuo Toraya, Tetsuo Kida, Atsushi Kuyama, Shinjiro Matsuda, Seiichi Tanaka, Yo Komatsu, Taro Tsurukai
Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary and resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative 1-MeAde receptors on the oocyte surface have been suggested, but not yet been biochemically characterized. Immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection, was attempted to detect unknown 1-MeAde binders including putative maturation-inducing hormone receptors in starfish oocytes...
March 25, 2017: Biochemical and Biophysical Research Communications
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