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https://www.readbyqxmd.com/read/27912163/reproduction-impairment-and-endocrine-disruption-in-adult-zebrafish-danio-rerio-after-waterborne-exposure-to-tboep
#1
Qinglong Xu, Ding Wu, Yao Dang, Liqin Yu, Chunsheng Liu, Jianghua Wang
Tris (2-butoxyethyl) phosphate (TBOEP) is widely used as a substitute of polybrominated diphenyl ethers (PBDEs). It has been frequently measured at concentrations of micrograms per liter (μg/L) in surface waters and waste water. However, limited information is available about the reproduction toxicology of TBOEP. In this study, adult zebrafish pairs were exposed to TBOEP at concentrations of 0, 5, 50, and 500μg/L for 21days. The effects on reproduction, hormone concentration, transcription of genes along the hypothalamic-pituitary-gonadal (HPG) axis, and gonadal development were investigated...
November 24, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#2
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27898723/pth1r-mutants-found-in-patients-with-primary-failure-of-tooth-eruption-disrupt-g-protein-signaling
#3
Hariharan Subramanian, Frank Döring, Sina Kollert, Natalia Rukoyatkina, Julia Sturm, Stepan Gambaryan, Angelika Stellzig-Eisenhauer, Philipp Meyer-Marcotty, Martin Eigenthaler, Erhard Wischmeyer
AIM: Primary failure of tooth eruption (PFE) is causally linked to heterozygous mutations of the parathyroid hormone receptor (PTH1R) gene. The mutants described so far lead to exchange of amino acids or truncation of the protein that may result in structural changes of the expressed PTH1R. However, functional effects of these mutations have not been investigated yet. MATERIALS AND METHODS: In HEK293 cells, PTH1R wild type was co-transfected with selected PTH1R mutants identified in patients with PFE...
2016: PloS One
https://www.readbyqxmd.com/read/27879451/tetraspanins-and-mouse-oocyte-microvilli-related-to-fertilizing-ability
#4
Achraf Benammar, Ahmed Ziyyat, Brigitte Lefèvre, Jean-Philippe Wolf
Our electron microscopy observations demonstrate for the first time that the number of microvilli on the mice oocyte membrane decreases when meiosis progresses from prophase I to metaphase II (MII) stage, and the morphology of the microvilli also changes. Microvilli are significantly shorter and larger on the ovulated oocyte membrane than at the previous stages. Although clathrin vesicles clearly disappear during oocyte maturation, exosome-like vesicles begin to be secreted at the metaphase I stage, more strongly at the MII stage...
November 21, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27857188/low-voltage-scanning-electron-microscopy-study-of-lampbrush-chromosomes-and-nuclear-bodies-in-avian-and-amphibian-oocytes
#5
Tatiana Kulikova, Tatiana Khodyuchenko, Yuri Petrov, Alla Krasikova
Nucleus is a highly compartmentalized part of the cell where the key processes of genome functionality are realized through the formation of non-membranous nuclear domains. Physically nuclear domains appear as liquid droplets with different viscosity stably maintained throughout the interphase or during the long diplotene stage of meiosis. Since nuclear body surface represents boundary between two liquid phases, the ultrastructural surface topography of nuclear domains is of an outstanding interest. The aim of this study was to examine ultrathin surface topography of the amphibian and avian oocyte nuclear structures such as lampbrush chromosomes, nucleoli, histone-locus bodies, Cajal body-like bodies, and the interchromatin granule clusters via low-voltage scanning electron microscopy...
November 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27822479/triflumuron-effects-on-the-physiology-and-reproduction-of-rhodnius-prolixus-adult-females
#6
Bianca Santos Henriques, Fernando Ariel Genta, Cícero Brasileiro Mello, Lucas Rangel Silva, Thaís Franco Codogno, Alyne F R Oliveira, Lourena Pinheiro Marinho, Denise Valle, José Bento Pereira Lima, Denise Feder, Marcelo Salabert Gonzalez, Patricia Azambuja
We evaluated the efficacy of the growth regulator triflumuron (TFM) in inducing mortality and disrupting both oviposition and egg hatching in Rhodnius prolixus adult females. TFM was administered via feeding, topically or by continuous contact with impregnated surfaces. Feeding resulted in mild biological effects compared with topical and impregnated surfaces. One day after treatment, the highest mortality levels were observed with topical surface and 30 days later both topical and impregnated surfaces induced higher mortalities than feeding...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27816448/egf-fsh-supplementation-reduces-apoptosis-of-pig-granulosa-cells-in-co-culture-with-cumulus-oocyte-complexes
#7
Maria Grazia Palmerini, Stefania Annarita Nottola, Woro Anidito Sri Tunjung, Akane Kadowaki, Serena Bianchi, Sandra Cecconi, Eimei Sato, Guido Macchiarelli
In cattle breeding, co-culture with granulosa cells (GCs) is one of the strategies to improve oocyte maturation and fertilization potential, but yields are still suboptimal due to GC apoptosis. We previously set up an in vitro co-culture system of cumulus-oocyte-complexes (COCs) anchored to GC multilayers adhering to the basal lamina (COCGs), in which GC apoptosis was inhibited by FSH supplementation. Here, we assessed the antiapoptotic effect of EGF (5 ng/ml-EGF5) alone or in synergism to FSH (50mU/ml-FSH50) on pig COCGs...
December 2, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27760355/extracellular-linkers-completely-transplant-the-voltage-dependence-from-kv1-2-ion-channels-to-kv2-1
#8
Fredrik Elinder, Michael Madeja, Hugo Zeberg, Peter Århem
The transmembrane voltage needed to open different voltage-gated K (Kv) channels differs by up to 50 mV from each other. In this study we test the hypothesis that the channels' voltage dependences to a large extent are set by charged amino-acid residues of the extracellular linkers of the Kv channels, which electrostatically affect the charged amino-acid residues of the voltage sensor S4. Extracellular cations shift the conductance-versus-voltage curve, G(V), by interfering with these extracellular charges...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27754867/regulation-of-trpp3-channel-function-by-n-terminal-domain-palmitoylation-and-phosphorylation
#9
Wang Zheng, JungWoo Yang, Erwan Beauchamp, Ruiqi Cai, Shaimaa Hussein, Laura Hofmann, Qiang Li, Veit Flockerzi, Luc G Berthiaume, Jingfeng Tang, Xing-Zhen Chen
Transient receptor potential polycystin-3 (TRPP3) is a cation channel activated by calcium and proton and is involved in hedgehog signaling, intestinal development, and sour tasting. How TRPP3 channel function is regulated remains poorly understood. By N-terminal truncation mutations, electrophysiology, and Xenopus oocyte expression, we first identified fragment Asp-21-Ser-42 to be functionally important. We then found that deletion mutant Δ1-36 (TRPP3 missing fragment Met-1-Arg-36) has a similar function as wild-type TRPP3, whereas Δ1-38 is functionally dead, suggesting the importance of Val-37 or Cys-38...
December 2, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27708759/a-mathematical-model-of-gtpase-pattern-formation-during-single-cell-wound-repair
#10
REVIEW
William R Holmes, Adriana E Golding, William M Bement, Leah Edelstein-Keshet
Rho GTPases are regulatory proteins whose patterns on the surface of a cell affect cell polarization, cell motility and repair of single-cell wounds. The stereotypical patterns formed by two such proteins, Rho and Cdc42, around laser-injured frog oocytes permit experimental analysis of GTPase activation, inactivation, segregation and crosstalk. Here, we review the development and analysis of a spatial model of GTPase dynamics that describe the formation of concentric zones of Rho and Cdc42 activity around wounds, and describe how this model has provided insights into the roles of the GTPase effector molecules protein kinase C (PKCβ and PKCη) and guanosine nucleotide dissociation inhibitor (GDI) in the wound response...
October 6, 2016: Interface Focus
https://www.readbyqxmd.com/read/27681563/structural-determinants-of-nh3-and-nh4-transport-by-mouse-rhbg-a-renal-rh-glycoprotein
#11
Solange Abdulnour-Nakhoul, Trang Le, Edd Rabon, L Lee Hamm, Nazih L Nakhoul
Renal Rhbg is localized to the basolateral membrane of intercalated cells and is involved in NH3/NH4(+) transport. The structure of Rhbg is not yet resolved; however, a high-resolution crystal structure of AmtB, a bacterial homolog of Rh, has been determined. We aligned the sequence of Rhbg to that of AmtB and identified important sites of Rhbg that may affect transport. Our analysis positioned three conserved amino acids, histidine 183 (H183), histidine 342 (H342), and tryptophan 230 (W230), within the hydrophobic pore where they presumably serve to control NH3 transport...
December 1, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27679865/characterization-of-tetraspanin-protein-cd81-in-mouse-spermatozoa-and-bovine-gametes
#12
Jana Jankovicova, Michaela Frolikova, Natasa Sebkova, Michal Simon, Petra Cupperova, Denisa Lipcseyova, Katarina Michalkova, Lubica Horovska, Radislav Sedlacek, Pavel Stopka, Jana Antalikova, Katerina Dvorakova-Hortova
Sperm-egg interaction and fusion represents a key moment of fertilization and it would not happen in mammals without the interaction of the tetraspanin superfamily members including protein CD81. A detailed immunohistochemical localization of CD81 was monitored on bovine oocytes of different maturation stages, as well as during early embryogenesis. There was also a CD81characterization on bovine and mouse sperm carried out. On bovine eggs, CD81 was detected on the plasma membrane of the germinal vesicle, metaphase I and metaphase II oocytes...
September 27, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27645113/scorpion-katoikogenic-ovariuterus-much-more-alike-to-apoikogenic-type-than-it-seemed-to-be
#13
Izabela Jędrzejowska, Kamil Szymusiak, Marta Mazurkiewicz-Kania, Arnold Garbiec
Scorpions are viviparous matrotrophic arthropods. Both, fertilization and embryonic development occur in the female gonad called ovariuterus. Two distinct reproductive patterns are recognized among scorpions: apoikogenic and katoikogenic. In the ovariuterus of apoikogenic scorpions growing oocytes protrude from the ovarian wall and continue previtellogenic and vitellogenic growth on the gonad surface being accompanied by the follicular cells that cover the oocyte surface, and, in most families, the stalk cells that join the oocyte with the ovariuterus wall...
September 27, 2016: Arthropod Structure & Development
https://www.readbyqxmd.com/read/27625611/the-calcium-sensing-receptor-and-the-reproductive-system
#14
REVIEW
Isabella Ellinger
Active placental transport of maternal serum calcium (Ca(2+)) to the offspring is pivotal for proper development of the fetal skeleton as well as various organ systems. Moreover, extracellular Ca(2+) levels impact on distinct processes in mammalian reproduction. The calcium-sensing receptor (CaSR) translates changes in extracellular Ca(2+)-concentrations into cellular reactions. This review summarizes current knowledge on the expression of CaSR and its putative functions in reproductive organs. CaSR was detected in placental cells mediating materno-fetal Ca(2+)-transport such as the murine intraplacental yolk sac (IPYS) and the human syncytiotrophoblast...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27624155/p2x-mediated-ampa-receptor-internalization-and-synaptic-depression-is-controlled-by-two-camkii-phosphorylation-sites-on-glua1-in-hippocampal-neurons
#15
Johan-Till Pougnet, Benjamin Compans, Audrey Martinez, Daniel Choquet, Eric Hosy, Eric Boué-Grabot
Plasticity at excitatory synapses can be induced either by synaptic release of glutamate or the release of gliotransmitters such as ATP. Recently, we showed that postsynaptic P2X2 receptors activated by ATP released from astrocytes downregulate synaptic AMPAR, providing a novel mechanism by which glial cells modulate synaptic activity. ATP- and lNMDA-induced depression in the CA1 region of the hippocampus are additive, suggesting distinct molecular pathways. AMPARs are homo-or hetero-tetramers composed of GluA1-A4...
September 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27582106/human-epithelial-na-channel-missense-variants-identified-in-the-gensalt-study-alter-channel-activity
#16
Evan C Ray, Jingxin Chen, Tanika N Kelly, Jiang He, L Lee Hamm, Dongfeng Gu, Lawrence C Shimmin, James E Hixson, Dabeeru C Rao, Shaohu Sheng, Thomas R Kleyman
Mutations in genes encoding subunits of the epithelial Na(+) channel (ENaC) can cause early onset familial hypertension, demonstrating the importance of this channel in modulating blood pressure. It remains unclear whether other genetic variants resulting in subtler alterations of channel function result in hypertension or altered sensitivity of blood pressure to dietary salt. This study sought to identify functional human ENaC variants to examine how these variants alter channel activity and to explore whether these variants are associated with altered sensitivity of blood pressure to dietary salt...
November 1, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27555600/protein-rs1-rsc1a1-downregulates-the-exocytotic-pathway-of-glucose-transporter-sglt1-at-low-intracellular-glucose-via-inhibition-of-ornithine-decarboxylase
#17
Chakravarthi Chintalapati, Thorsten Keller, Thomas D Mueller, Valentin Gorboulev, Nadine Schäfer, Ilona Zilkowski, Maike Veyhl-Wichmann, Dietmar Geiger, Jürgen Groll, Hermann Koepsell
Na(+)-d-glucose cotransporter 1 (SGLT1) is rate-limiting for glucose absorption in the small intestine. Shortly after intake of glucose-rich food, SGLT1 abundance in the luminal membrane of the small intestine is increased. This upregulation occurs via glucose-induced acceleration of the release of SGLT1-containing vesicles from the trans-Golgi network (TGN), which is regulated by a domain of protein RS1 (RSC1A1) named RS1-Reg. Dependent on phosphorylation, RS1-Reg blocks release of vesicles containing SGLT1 or concentrative nucleoside transporter 1...
November 2016: Molecular Pharmacology
https://www.readbyqxmd.com/read/27518902/hira-is-required-for-heart-development-and-directly-regulates-tnni2-and-tnnt3
#18
Daniel Dilg, Rasha Noureldin M Saleh, Sarah Elizabeth Lee Phelps, Yoann Rose, Laurent Dupays, Cian Murphy, Timothy Mohun, Robert H Anderson, Peter J Scambler, Ariane L A Chapgier
Chromatin remodelling is essential for cardiac development. Interestingly, the role of histone chaperones has not been investigated in this regard. HIRA is a member of the HUCA (HIRA/UBN1/CABIN1/ASF1a) complex that deposits the variant histone H3.3 on chromatin independently of replication. Lack of HIRA has general effects on chromatin and gene expression dynamics in embryonic stem cells and mouse oocytes. Here we describe the conditional ablation of Hira in the cardiogenic mesoderm of mice. We observed surface oedema, ventricular and atrial septal defects and embryonic lethality...
2016: PloS One
https://www.readbyqxmd.com/read/27500836/control-of-oocyte-reawakening-by-kit
#19
Hatice Duygu Saatcioglu, Ileana Cuevas, Diego H Castrillon
In mammals, females are born with finite numbers of oocytes stockpiled as primordial follicles. Oocytes are "reawakened" via an ovarian-intrinsic process that initiates their growth. The forkhead transcription factor Foxo3 controls reawakening downstream of PI3K-AKT signaling. However, the identity of the presumptive upstream cell surface receptor controlling the PI3K-AKT-Foxo3 axis has been questioned. Here we show that the receptor tyrosine kinase Kit controls reawakening. Oocyte-specific expression of a novel constitutively-active KitD818V allele resulted in female sterility and ovarian failure due to global oocyte reawakening...
August 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27490311/the-hydroxysteroid-17%C3%AE-dehydrogenase-family-gene-hsd17b12-is-involved-in-the-prostaglandin-synthesis-pathway-the-ovarian-function-and-regulation-of-fertility
#20
Heidi Kemiläinen, Marion Adam, Jenni Mäki-Jouppila, Pauliina Damdimopoulou, Anastasios E Damdimopoulos, Juha Kere, Outi Hovatta, Teemu D Laajala, Tero Aittokallio, Jerzy Adamski, Henrik Ryberg, Claes Ohlsson, Leena Strauss, Matti Poutanen
The hydroxysteroid (17beta) dehydrogenase (HSD17B)12 gene belongs to the hydroxysteroid (17β) dehydrogenase superfamily, and it has been implicated in the conversion of estrone to estradiol as well as in the synthesis of arachidonic acid (AA). AA is a precursor of prostaglandins, which are involved in the regulation of female reproduction, prompting us to study the role of HSD17B12 enzyme in the ovarian function. We found a broad expression of HSD17B12 enzyme in both human and mouse ovaries. The enzyme was localized in the theca interna, corpus luteum, granulosa cells, oocytes, and surface epithelium...
October 2016: Endocrinology
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