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https://www.readbyqxmd.com/read/29321627/publisher-correction-a-cdk1-gradient-guides-surface-contraction-waves-in-oocytes
#1
Johanna Bischof, Christoph A Brand, Kálmán Somogyi, Imre Májer, Sarah Thome, Masashi Mori, Ulrich S Schwarz, Péter Lénárt
A Supplementary Information file from a different paper was inadvertently published with the original version of this Article. This file was replaced with the correct Supplementary Information file on 24 October 2017.
January 10, 2018: Nature Communications
https://www.readbyqxmd.com/read/29312650/nanoparticles-and-female-reproductive-system-how-do-nanoparticles-affect-oogenesis-and-embryonic-development
#2
REVIEW
Cong-Cong Hou, Jun-Quan Zhu
Along with the increasing application of nanoparticles (NPs) in many walks of life, environmental exposure to NPs has raised considerable health concerns. When NPs enter a pregnant woman's body through inhalation, venous injection, ingestion or skin permeation, maternal toxic stress reactions such as reactive oxygen species (ROS), inflammation, apoptosis and endocrine dyscrasia are induced in different organs, particularly in the reproductive organs. Recent studies have shown that NPs disturb the developing oocyte by invading the protective barrier of theca cells, granulosa cell layers and zona pellucida...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29306179/vector-mediated-transmission-of-persistently-transmitted-plant-viruses
#3
REVIEW
Dongsheng Jia, Qian Chen, Qianzhuo Mao, Xiaofeng Zhang, Wei Wu, Hongyan Chen, Xiangzhen Yu, Zhiqiang Wang, Taiyun Wei
Many vector-borne plant viruses of agricultural importance are persistently transmitted from plant to plant by sap-sucking insects. So far, the mechanisms for vector-mediated horizontal transmission of the viruses to plant hosts and for vertical transmission to insect offspring have been poorly understood. During horizontal transmission, intact virions or virus-induced inclusions are exploited by persistently transmitted viruses to overcome the midgut and salivary gland barriers. The existing oocyte entry paths used by vitellogenin or symbiont bacteria can mediate the vertical transmission of viruses by female insects...
January 3, 2018: Current Opinion in Virology
https://www.readbyqxmd.com/read/29207252/role-of-activin-c-in-normal-ovaries-and-granulosa-cell-tumours-of-mice-and-humans
#4
Karen L Reader, Francesco E Marino, Helen D Nicholson, Gail P Risbridger, Elspeth J Gold
Activins and inhibins play important roles in the development, growth and function of the ovary. Mice lacking inhibin develop granulosa cell tumours in their ovaries that secrete activin A, and these tumours are modulated by increased activin C expression. The aim of the present study was to identify where activin C is expressed in mouse and human ovaries and whether overexpression of activin C modulates normal follicular development in mice. Immunohistochemical staining for the activin βC subunit was performed on sections from mouse and human ovaries and human adult granulosa cell tumours...
December 6, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29207017/a-common-african-variant-of-human-connexin%C3%A2-37-is-associated-with-caucasian-primary-ovarian-insufficiency-and-has-a-deleterious-effect-in%C3%A2-vitro
#5
Anne Bachelot, Jerome Gilleron, Geri Meduri, Mihelai Guberto, Jerome Dulon, Sylviane Boucherie, Philippe Touraine, Micheline Misrahi
Folliculogenesis requires communication between granulosa cells and oocytes, mediated by connexin-based gap junctions. Connexin 37 (Cx37)-deficient female mice are infertile. The present study assessed Cx37 deficiency in patients with primary ovarian insufficiency (POI). A candidate gene study was performed in patients and controls from the National Genotyping Center (Evry, France) including 58 Caucasian patients with idiopathic isolated POI and 142 Caucasian controls. Direct genomic sequencing of the coding regions of the GJA4 gene (encoding Cx37) was performed with the aim to identify a deleterious variant associated with POI and absent in ethnically matched controls...
November 16, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29191251/grooves-surrounding-the-micropyle-decrease-the-inseminating-dose-in-fish
#6
Matheus Pereira-Santos, Eduardo Shimoda, André Furugen Cesar de Andrade, Luciano Andrade Silva, Takafumi Fujimoto, José Augusto Senhorini, George Shigueki Yasui, Laura Satiko Okada Nakaghi
In fish with external fertilization, sperm must reach the oocyte through the micropyle to enter the cytoplasm. Fertilization success is then influenced by characteristics of oocytes or sperm. In this study, we evaluated oocyte morphology and sperm motility parameters and their effects on the inseminating dose in a teleost fish Astyanax altiparanae. Interestingly, we found one of the lowest yet described inseminating doses in teleosts (2390 spermatozoa oocyte-1 ml-1). Such a fertilization efficacy may be explained by the long duration of sperm motility (>75 s), the small oocyte diameter (695...
December 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29187368/n-linked-glycans-are-required-on-epithelial-na-channel-subunits-for-maturation-and-surface-expression
#7
Ossama B Kashlan, Carol L Kinlough, Michael M Myerburg, Shujie Shi, Jingxin Chen, Brandon M Blobner, Teresa M Buck, Jeffrey L Brodsky, Rebecca P Hughey, Thomas R Kleyman
Epithelial Na+ channel (ENaC) subunits undergo N-linked glycosylation in the endoplasmic reticulum where they assemble into an αβγ complex. Six, thirteen and five consensus sites (Asn-x-Ser/Thr) for N-glycosylation reside in the extracellular domains of the mouse α, β and γ subunits, respectively. Because the importance of ENaC N-linked glycans has not been fully addressed, we examined the effect of preventing N-glycosylation of specific subunits on channel function, expression, maturation, and folding...
November 29, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29184995/quantification-of-size-effect-on-protein-rotational-mobility-in-cells-by-19f-nmr-spectroscopy
#8
Yansheng Ye, Qiong Wu, Wenwen Zheng, Bin Jiang, Gary J Pielak, Maili Liu, Conggang Li
Protein diffusion in living cells might differ significantly from that measured in vitro. Little is known about the effect of globular protein size on rotational diffusion in cells because each protein has distinct surface properties, which result in different interactions with cellular components. To overcome this problem, the B1 domain of protein G (GB1) and several concatemers of the protein were labeled with 5-fluorotryptophan and studied by 19F NMR in Escherichia coli cells, Xenopus laevis oocytes, and in aqueous solutions crowded with glycerol, or Ficoll70™ and lysozyme...
November 28, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29179200/stress-kinase-regulation-of-task-1-and-task-3
#9
Susanne Rinné, Aytug K Kiper, Constanze Schmidt, Beatriz Ortiz-Bonnin, Simone Zwiener, Guiscard Seebohm, Niels Decher
BACKGROUND/AIMS: TASK channels belong to the two-pore-domain potassium (K2P) channel family. TASK-1 is discussed to contribute to chronic atrial fibrillation (AFib) and has been together with uncoupling protein 1 found as a marker protein of brown adipose tissue (BAT) fat. In addition, TASK-1 was linked in a genome-wide association study to an increased body mass index. A recent study showed that TASK-1 inhibition is causing obesity in mice by a BAT whitening and that these effects are linked to the mineralocorticoid receptor pathway, albeit the mechanism remained elusive...
November 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29168928/regulation-of-kv1-4-potassium-channels-by-pkc-and-ampk-kinases
#10
Martin Nybo Andersen, Lasse Skibsbye, Arnela Saljic, Martin Zahle Larsen, Hanne Borger Rasmussen, Thomas Jespersen
Over the last years extensive kinase-mediated regulation of a number of voltage-gated potassium (Kv) channels important in cardiac electrophysiology has been reported. This includes regulation of Kv1.5, Kv7.1 and Kv11.1 cell surface expression, where the kinase-mediated regulation appears to center around the ubiquitin ligase Nedd4-2. In the present study we examined whether Kv1.4, constituting the cardiac Ito,s current, is subject to similar regulation. In the epithelial Madin-Darby Canine Kidney (MDCK) cell line, which constitutes a highly reproducible model system for addressing membrane targeting, we find, by confocal microscopy, that Kv1...
November 23, 2017: Channels
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#11
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
January 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29128912/ovarian-stem-cells-resolving-controversies
#12
Deepa Bhartiya, Hiren Patel
A recent review on ovarian stem cells by Horan and Williams entitled "Oocyte Stem Cells: Fact or Fantasy?" suggests that the debate on ovarian stem cells (OSCs) is still not over. They did not even discuss the presence of two distinct populations of stem cells in the ovary in their review. OSCs are located in the ovary surface epithelium and Tilly's group reported them in the size range of 5-8 μm whereas Virant-Klun's group has reported pluripotent, 2-4 μm OSCs. Our group reported OSCs of two distinct sizes including pluripotent very small embryonic-like stem cells (VSELs) which are smaller in size than RBCs (similar to those reported by Virant-Klun's group) and slightly bigger (similar to those reported by Tilly's group) tissue committed progenitors (OSCs) that presumably differentiate from VSELs...
November 11, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29126465/facile-synthesis-of-magnetic-metal-organic-frameworks-for-highly-efficient-proteolytic-digestion-used-in-mass-spectrometry-based-proteomics
#13
Rui Zhai, Yifeng Yuan, Fenglong Jiao, Feiran Hao, Xiang Fang, Yangjun Zhang, Xiaohong Qian
Mass spectrometry (MS) based bottom-up strategy is now the first choice for proteomics analysis. In this process, highly efficient and complete enzymatic degradation of protein samples is extremely important to achieve in-depth protein coverage and high-throughput protein profiling. However, conventional in-solution digestion suffer from long digestion time and enzyme autolysis that limit the protein sample processing throughput and identification accuracy. Here, we developed a novel type of magnetic metal organic frameworks (MOFs)-based immobilized enzyme reactor, Fe3O4@DOTA-ZIF-90-trypsin...
November 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29124919/location-and-expression-of-juno-in-mice-oocytes-during-maturation
#14
Banri Suzuki, Yukou Sugano, Jun Ito, Haruka Saito, Sueo Niimura, Hideaki Yamashiro
OBJECTIVE: Oocyte-sperm interaction is the essential step in fertilization. Juno, which has been known as Folate receptor 4, is the Izumo1 receptor expressed on the oocyte membrane. This study aims to investigate the location and expression of Juno in mice oocytes during maturation. METHODS: To confirm the stage at which Juno expression begins in the mice oocytes and its location pattern, we performed immunostaining methods. Next, we evaluated Juno mRNA expression by a half quantitative RT-PCR...
November 10, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29102625/reprint-of-role-of-g-protein-coupled-estrogen-receptor-gper-gpr30-in-maintenance-of-meiotic-arrest-in-fish-oocytes
#15
REVIEW
Peter Thomas
An essential role for GPER (formerly known as GPR30) in regulating mammalian reproduction has not been identified to date, although it has shown to be involved in the regulation a broad range of other estrogen-dependent functions. In contrast, an important reproductive role for GPER in the maintenance of oocyte meiotic arrest has been identified in teleost fishes, which is briefly reviewed here. Recent studies have clearly shown that ovarian follicle production of estradiol-17β (E2) maintains meiotic arrest in several teleost species through activation of GPER coupled to a stimulatory G protein (Gs) on oocyte plasma membranes, resulting in stimulation of cAMP production and maintenance of elevated cAMP levels...
November 2, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29099693/comparative-analysis-of-sperm-selection-methods-before-the-intra-cytoplasmic-sperm-injection-procedure
#16
O Lohinova, V Pitko, N Sinilo, Ya Cherkashyna, N Hanzha
In recent years, one of the methods of assisted reproductive technologies has become increasingly widespread, namely the ICSI procedure (ICSI - Intra Cytoplasmic Sperm Injection). This method is based on artificial introduction of the spermatozoon into the cytoplasm of the oocyte. However, the complexity of this method is that the choice of a morphologically and biochemically mature spermatozoon becomes subjective. However, not so long ago, an innovative method for selecting mature, ready-to-fertilize spermatozoa was proposed...
October 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29055709/thioredoxin-reductase-from-the-bovine-filarial-parasite-setaria-cervi-studies-on-its-localization-and-optimization-of-the-extraction
#17
Nikhilesh Joardar, Suprabhat Mukherjee, Santi P Sinha Babu
Exploration of novel drug targets has been the major thrust area in filarial research. In this regard, identification and characterization of oxidative enzymes that play pivotal role in the survival of filarial parasite inside host are of immense importance. In this study, we are reporting the presence of an important redox regulatory enzyme, thioredoxin reductase (TrxR) in the bovine filarial parasite Setaria cervi. TrxR was found to be exists throughout the developmental stages viz. oocyte, microfilaria and adult of the parasite...
October 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29037933/role-of-maternal-xenopus-syntabulin-in-germ-plasm-aggregation-and-primordial-germ-cell-specification
#18
Denise Oh, Douglas W Houston
The localization and organization of mitochondria- and ribonucleoprotein granule-rich germ plasm is essential for many aspects of germ cell development. In Xenopus, germ plasm is maternally inherited and is required for the specification of primordial germ cells (PGCs). Germ plasm is aggregated into larger patches during egg activation and cleavage and is ultimately translocated perinuclearly during gastrulation. Although microtubule dynamics and a kinesin (Kif4a) have been implicated in Xenopus germ plasm localization, little is known about how germ plasm distribution is regulated...
October 14, 2017: Developmental Biology
https://www.readbyqxmd.com/read/29025042/stage-specific-follicular-extracellular-vesicle-uptake-and-regulation-of-bovine-granulosa-cell-proliferation
#19
Wei-Ting Hung, Raphatphorn Navakanitworakul, Tarique Khan, Pan Zhang, John S Davis, Lynda K McGinnis, Lane K Christenson
Follicular fluid within ovarian antral follicles contains numerous factors, which influence the development of a healthy oocyte including nucleic acids, steroids, proteins, and extracellular vesicles (EVs). Current evidence indicates that follicular EVs promote changes in cellular gene expression and support cumulus-oocyte complex expansion in vitro. In this study, we found EVs from different sized follicles differentially stimulate granulosa cell proliferation and this could be explained by both the differential contents associated, on or within the vesicles and by the preferential uptake of EVs dependent on follicle size from which they were isolated...
October 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29021609/a-cdk1-gradient-guides-surface-contraction-waves-in-oocytes
#20
Johanna Bischof, Christoph A Brand, Kálmán Somogyi, Imre Májer, Sarah Thome, Masashi Mori, Ulrich S Schwarz, Péter Lénárt
Surface contraction waves (SCWs) in oocytes and embryos lead to large-scale shape changes coupled to cell cycle transitions and are spatially coordinated with the cell axis. Here, we show that SCWs in the starfish oocyte are generated by a traveling band of myosin II-driven cortical contractility. At the front of the band, contractility is activated by removal of cdk1 inhibition of the RhoA/RhoA kinase/myosin II signaling module, while at the rear, contractility is switched off by negative feedback originating downstream of RhoA kinase...
October 11, 2017: Nature Communications
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