keyword
MENU ▼
Read by QxMD icon Read
search

Sperm surface

keyword
https://www.readbyqxmd.com/read/29198215/dietary-l-arginine-supplementation-improves-semen-quality-and-libido-of-boars-under-high-ambient-temperature
#1
J Q Chen, Y S Li, Z J Li, H X Lu, P Q Zhu, C M Li
l-Arginine is a nutritionally essential amino acid for spermatogenesis and plays versatile roles in animal health and can be utilized as a potential agent to improve reproductive performance of boars under high ambient temperature. The present study aimed to determine whether dietary l-arginine could alleviate heat stress-induced infertility in boars. In all, 20 boars (PIC 1040; 248.59±3.84 kg BW and 407.65±6.40 days of age) were selected and randomly assigned to four groups (group 0.0%, basal diet; group 0...
December 4, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29191251/grooves-surrounding-the-micropyle-decrease-the-inseminating-dose-in-fish
#2
Matheus Pereira-Santos, Eduardo Shimoda, André Furugen Cesar de Andrade, Luciano Andrade Silva, Takafumi Fujimoto, José Augusto Senhorini, George Shigueki Yasui, Laura Satiko Okada Nakaghi
In fish with external fertilization, sperm must reach the oocyte through the micropyle to enter the cytoplasm. Fertilization success is then influenced by characteristics of oocytes or sperm. In this study, we evaluated oocyte morphology and sperm motility parameters and their effects on the inseminating dose in a teleost fish Astyanax altiparanae. Interestingly, we found one of the lowest yet described inseminating doses in teleosts (2390 spermatozoa oocyte-1 ml-1). Such a fertilization efficacy may be explained by the long duration of sperm motility (>75 s), the small oocyte diameter (695...
December 1, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29159832/expression-and-localisation-of-rxfp3-in-human-spermatozoa-and-impact-of-insl7-on-sperm-functions
#3
S Heidari, A H Taromchi, R Nejatbakhsh, S Shokri
Insulin-like peptide 7 (INSL7) or relaxin-3 is a member of the insulin superfamily that is recently discovered. This hormone interacts with relaxin family peptide receptor 3 (RXFP3). Although recent studies of INSL7 have focused on its function in the brain as a neuropeptide, spermatozoa may be a candidate target of INSL7 due to its detection in testes and contains binding sites. Therefore, this study aims to analyse the expression and localisation of RXFP3 on human spermatozoa and to assess the effect of INSL7 on human sperm motility...
November 21, 2017: Andrologia
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#4
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
November 15, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29145645/waddlia-chondrophila-a-chlamydia-related-bacterium-has-a-negative-impact-on-human-spermatozoa
#5
D Baud, N Vulliemoz, A Ammerdorffer, J Gyger, G Greub, V Castella, M Stojanov
STUDY QUESTION: What is the impact of Waddlia chondrophila, an emerging Chlamydia-related bacterium associated with miscarriage, on human spermatozoa? SUMMARY ANSWER: W. chondrophila had a negative impact on human spermatozoa (decrease in viability and mitochondrial membrane potential) and was not entirely removed from infected samples by density gradient centrifugation. WHAT IS KNOWN ALREADY: Bacterial infection or colonization might have a deleterious effect on male fertility...
November 14, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29124919/location-and-expression-of-juno-in-mice-oocytes-during-maturation
#6
Banri Suzuki, Yukou Sugano, Jun Ito, Haruka Saito, Sueo Niimura, Hideaki Yamashiro
OBJECTIVE: Oocyte-sperm interaction is the essential step in fertilization. Juno, which has been known as Folate receptor 4, is the Izumo1 receptor expressed on the oocyte membrane. This study aims to investigate the location and expression of Juno in mice oocytes during maturation. METHODS: To confirm the stage at which Juno expression begins in the mice oocytes and its location pattern, we performed immunostaining methods. Next, we evaluated Juno mRNA expression by a half quantitative RT-PCR...
November 10, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29123094/human-sperm-steer-with-second-harmonics-of-the-flagellar-beat
#7
Guglielmo Saggiorato, Luis Alvarez, Jan F Jikeli, U Benjamin Kaupp, Gerhard Gompper, Jens Elgeti
Sperm are propelled by bending waves traveling along their flagellum. For steering in gradients of sensory cues, sperm adjust the flagellar waveform. Symmetric and asymmetric waveforms result in straight and curved swimming paths, respectively. Two mechanisms causing spatially asymmetric waveforms have been proposed: an average flagellar curvature and buckling. We image flagella of human sperm tethered with the head to a surface. The waveform is characterized by a fundamental beat frequency and its second harmonic...
November 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/29116153/ardissonea-crystallina-has-a-type-of-sexual-reproduction-that-is-unusual-for-centric-diatoms
#8
Nickolai A Davidovich, Olga I Davidovich, Yulia A Podunay, Romain Gastineau, Irena Kaczmarska, Aloisie Poulíčková, Andrzej Witkowski
Molecular phylogenetic analyses place Ardissonea crystallina (C. Agardh) Grunow and all Toxariids among the bi- and multipolar centric diatoms, almost always recovered as a derived lineage sister to Lampriscus. In all centrics where sexual reproduction has been documented, oogamy, with larger immobile eggs and smaller flagellated sperm has been observed. We were able to initiate both homothallic and heterothallic reproduction in A. crystallina. The heterothallic reproduction turned out to be non-oogamous; gametes were more or less equal in size but no flagellated cells were detected...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29099693/comparative-analysis-of-sperm-selection-methods-before-the-intra-cytoplasmic-sperm-injection-procedure
#9
O Lohinova, V Pitko, N Sinilo, Ya Cherkashyna, N Hanzha
In recent years, one of the methods of assisted reproductive technologies has become increasingly widespread, namely the ICSI procedure (ICSI - Intra Cytoplasmic Sperm Injection). This method is based on artificial introduction of the spermatozoon into the cytoplasm of the oocyte. However, the complexity of this method is that the choice of a morphologically and biochemically mature spermatozoon becomes subjective. However, not so long ago, an innovative method for selecting mature, ready-to-fertilize spermatozoa was proposed...
October 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29065974/seminal-plasma-and-cryopreservation-alter-ram-sperm-surface-carbohydrates-and-interactions-with-neutrophils
#10
Taylor Pini, Tamara Leahy, Simon Paul de Graaf
Spermatozoa deposited vaginally must navigate the physical, chemical and immune barriers of the cervix to reach the site of fertilisation. Characteristics that favour successful cervical transit remain largely unknown beyond the obvious factors of motility and viability. Epididymal and cryopreserved ram spermatozoa demonstrate poor cervical transit, for unknown reasons. We hypothesised that seminal plasma exposure and cryopreservation alter the surface sugars of these sperm populations and, consequently, their interaction with immune cells, both potential factors for successful cervical transit...
October 25, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29063176/evaluation-of-protein-phosphorylation-in-bull-sperm-during-their-maturation-in-the-epididymis
#11
Jana Jankovičová, Katarína Michalková, Petra Sečová, Ľubica Horovská, Pavla Maňásková-Postlerová, Jana Antalíková
Phosphorylation, or dephosphorylation, is one of the most frequent post-translational modifications regulating protein-protein activity in eukaryotic cells. Whereas mature spermatozoa (as specialized cells) are transcriptionally inactive and do not synthesize new proteins, phosphorylation of sperm proteins is very important for the regulation of the sperm function. Although the post-testicular maturation of spermatozoa is a process common to all mammals, comparative studies showed significant differences in sperm surface proteins and the mechanisms of protein modification during the epididymal maturation...
October 23, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29045572/impaired-sperm-maturation-in-conditional-lcn6-knockout-mice
#12
Qianqian Yin, Jia Shen, Xiaofeng Wan, Qiang Liu, Yuchuan Zhou, Yonglian Zhang
Human LCN6, a lipocalin protein, exhibits predominant expression in epididymis and location on the sperm surface. However, the biological function of LCN6 in vivo remains unknown. Herein, we found that unlike human LCN6, mouse Lcn6 gene encoded two transcript variants that were both up regulated by androgen. Subsequently, we generated a conditional knockout mouse model to disrupt Lcn6 in the adult and investigate its function. In this model, spermatogenesis was normal and Lcn6 deficiency did not affect the natural birth rate of male mice or in vitro fertilization ability of their cauda epididymal sperm...
October 13, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28971894/clusterin-in-the-mouse-epididymis-possible-roles-in-sperm-maturation-and-capacitation
#13
Arpornrad Saewu, Suraj Kadunganattil, Riya Raghupathy, Kessiri Kongmanas, Pamela Diaz Astudillo, Louis Hermo, Nongnuj Tanphaichitr
Clusterin (CLU) is known as an extracellular chaperone for proteins under stress, thus preventing them from aggregation and precipitation. We showed herein that CLU, expressed by principal cells of the mouse caput epididymis, was present in high amounts in the lumen. In the cauda epididymis, CLU bound tightly to the sperm head surface and its amount on total sperm was similar to that in the bathing luminal fluid. In both immotile and motile caudal epididymal sperm, CLU was localized over the entire sperm head except at the convex ridge, although in the motile sperm population the CLU immunofluorescence pattern was distinctively mottled with a lower intensity...
October 2, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28962037/assessment-of-sperm-nuclear-quality-after-in-vitro-maturation-of-fresh-or-frozen-thawed-mouse-pre-pubertal-testes
#14
A Oblette, N Rives, L Dumont, A Rives, F Verhaeghe, F Jumeau, C Rondanino
STUDY QUESTION: Is nuclear quality of in vitro generated spermatozoa from fresh or frozen/thawed pre-pubertal mouse testes similar to that of their in vivo counterparts? SUMMARY ANSWER: The production of spermatozoa with aneuploidy, DNA fragmentation or chromatin condensation defects was not significantly increased in organotypic cultures compared to in vivo controls. WHAT IS KNOWN ALREADY: Although murine spermatozoa have been produced in vitro from pre-pubertal testes, their nuclear DNA integrity has never been investigated...
August 23, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28942506/production-of-tiger-puffer-takifugu-rubripes-offspring-from-triploid-grass-puffer-takifugu-niphobles-parents
#15
Masaomi Hamasaki, Yutaka Takeuchi, Ryosuke Yazawa, Souta Yoshikawa, Kazushi Kadomura, Toshiyuki Yamada, Kadoo Miyaki, Kiyoshi Kikuchi, Goro Yoshizaki
The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2-5 kg) much larger than average market size (0.6-1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer...
September 23, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28903832/validation-of-modifications-to-the-ansr-for-listeria-method-for-improved-internal-positive-control-performance
#16
Susan Alles, Evan Meister, Edan Hosking, Eric Tovar, Rebecca Shaulis, Mark Schonfeld, Lei Zhang, Lin Li, Preetha Biswas, Mark Mozola, Robert Donofrio
A study was conducted to validate a minor reagent formulation change to the ANSR for Listeria method, Performance Tested Method(SM) 101202. This change involves increasing the master mix volume prelyophilization by 40% and addition of salmon sperm DNA (nontarget DNA) to the master mix. These changes improve the robustness of the internal positive control response and reduce the possibility of obtaining invalid results due to weak-positive control curves. When three foods (hot dogs, Mexican-style cheese, and cantaloupe) and sponge samples taken from a stainless steel surface were tested, no significant differences in performance between the ANSR and U...
September 14, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28878960/eversion-and-withdrawal-of-an-intromittent-organ-before-sexual-maturation-prepares-male-beetles-for-copulation
#17
Yoko Matsumura, Takuya Kubo
Some species of criocerine beetles have a hyper-elongated part of the intromittent organ called a flagellum. In resting position, the flagellum is stored in a specialized internal sac in the intromittent organ. This specialized state of the flagellum and internal sac is indispensable during copulation for flagellar insertion into the female spermathecal duct for sperm transfer. However, the morphogenesis of the flagellum does not generate the active state of the flagellum; rather, the flagellum is generated in an inactive and completely coiled state...
August 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28843076/epididymal-%C3%AE-l-fucosidase-and-its-possible-role-in-remodelling-the-surface-of-bull-spermatozoa
#18
Andrea Carolina Aguilera, Veronica Boschin, Inmaculada Robina, Pilar Elías-Rodríguez, Miguel Angel Sosa
The mammalian epididymis provides an appropriate environment for sperm maturation. During the epididymal transit, spermatozoa undergo biochemical and morphological changes that lead to the acquisition of the fertilizing capacity. In this study we analysed the fucosylation status of membrane glycoproteins in the spermatozoa obtained from different regions of the bull epididymis. High amounts of fucose were detected on caput spermatozoa (R.F.I. = 1010 ± 20.35), mostly located in the post-acrosome zone. A significant decrease in the fucose levels was detected toward the cauda (R...
August 19, 2017: Theriogenology
https://www.readbyqxmd.com/read/28806153/expression-purification-and-refolding-of-human-lipocalin-6-and-production-of-a-monoclonal-antibody-against-this-protein
#19
Jiong Chen, Wei Feng, Yue Zhao, Yaqin Li, Fei Zhan
Human lipocalin 6 (hLCN6) is a member of the lipocalin family, which is a group of structurally conserved hydrophobic ligand binding proteins, and widely distributed in animal, plant, and bacteria. Specific expression of hLCN6 in the epididymis and localization of this protein on the surface of spermatozoa suggest a role played by hLCN6, which may function as a transporter to carry ligands in the epididymal channel. However, the role of hLCN6 in sperm maturation has been largely unknown due to the lack of effective antibodies...
August 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28805603/modern-human-sperm-freezing-effect-on-dna-chromatin-and-acrosome-integrity
#20
Tahereh Rahiminia, Akram Hosseini, Morteza Anvari, Saeed Ghasemi-Esmailabad, Ali Reza Talebi
OBJECTIVE: Presence of vitrification method in sperm freezing and the introduction of solid surface vitrification beside rapid freezing in vapour, opens an easy and safe way to help infertility centres. While the effects of cryopreservation on motility, morphology and viability of sperm are documented, the question of the probable alteration of sperm DNA, chromatin and acrosome integrity after freezing and thawing procedures in different methods is still controversial. MATERIALS AND METHODS: Normal sample were collected according to WHO strict criteria...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
keyword
keyword
94658
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"