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https://www.readbyqxmd.com/read/29672554/inhibition-of-sperm-motility-in-male-macaques-with-ep055-a-potential-non-hormonal-male-contraceptive
#1
Michael G O'Rand, Katherine G Hamil, Tiffany Adevai, Mary Zelinski
Men have two practical choices for contraception; the condom which has a high typical use failure rate or vasectomy. New male hormonal and non-hormonal contraceptives are under development that target either the production of sperm (spermatogenesis) or the delivery of sperm. One particular target is the sperm protein EPPIN, which is present on the surface of human spermatozoa. EP055 is a small organic compound that targets EPPIN on the surface of sperm and inhibits motility. EP055 was tested in cynomolgus (Macaca fascicularis) males to determine its plasma half-life after intravenous (i...
2018: PloS One
https://www.readbyqxmd.com/read/29657075/seminal-plasma-proteomes-and-sperm-fertility
#2
REVIEW
Xavier Druart, Simon de Graaf
During ejaculation, the spermatozoa are transported by the seminal plasma, a fluid resulting from secretions originating mainly from the prostate and the seminal vesicles in mammals. The interaction of the seminal plasma with spermatozoa induces binding of seminal proteins onto the sperm surface and membrane remodeling potentially impacting the sperm transport, survival and fertilizing ability in the female genital tract. The seminal plasma also contains peptides and proteins involved in the inflammatory and immune response of the female tract...
April 10, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29653077/binder-of-sperm-proteins-protect-ram-spermatozoa-from-freeze-thaw-damage
#3
Taylor Pini, Kiri Farmer, Xavier Druart, Ana Paula Teixeira-Gomes, Guillaume Tsikis, Valerie Labas, Tamara Leahy, Simon P de Graaf
Cryopreservation causes sub-lethal damage which limits the fertility of frozen thawed spermatozoa. Seminal plasma has been investigated as a cryoprotectant, but has yielded inconsistent results due to considerable variation in its constituents. Individual seminal plasma proteins offer an ideal alternative to whole seminal plasma, and several have been correlated with freezing success. Binder of Sperm Proteins (BSPs) are abundant ram seminal plasma proteins which have been suggested to have significant protective effects on ram spermatozoa during cold shock...
April 10, 2018: Cryobiology
https://www.readbyqxmd.com/read/29622351/proteomics-a-valuable-approach-to-elucidate-spermatozoa-post-testicular-maturation-in-the-endangered-acipenseridae-family
#4
REVIEW
Pietro Boccaletto, Mohammad Abdul Momin Siddique, Jacky Cosson
Proteomics techniques, such as two-dimensional polyacrylamide gel electrophoresis, mass spectrometry, and differential gel electrophoresis, have been extensively used to describe the protein composition of male gametes in different animals, mainly mammals. They have also provided a deeper understanding of protein functions involved in sperm processes, as in processes that in humans lead to male infertility. However, few studies focus on fish sperm proteomics and even fewer have tried to explore the proteomic profile of Sturgeon spermatozoa...
March 31, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29618635/removal-of-sialic-acid-from-bull-sperm-decreases-motility-and-mucus-penetration-ability-but-increases-zona-pellucida-binding-and-polyspermic-penetration-in-vitro
#5
Beatriz Fernandez-Fuertes, Alfonso Blanco-Fernandez, Colm J Reid, Kieran Meade, S Fair, Pat Lonergan
This study tested the hypothesis that sperm sialic acid (Sia) is required to reach the site of fertilization, and that successful fertilization requires recognition of Sia from both the sperm and oocyte to occur. In addition, it has recently been reported that Siglecs (Sia-binding-immunoglobulin-like lectins) are present on the sperm surface. Thus, the possibility that the recognition of oocyte Sia was sperm-Siglec-mediated was also addressed. Sperm exposed to neuraminidase (NMase) exhibited lower overall and progressive motility, which translated to a decreased ability to swim through cervical mucus from cows in oestrus...
April 4, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29617298/lectin-binding-specificity-of-the-fertilization-relevant-protein-pdc-109-by-means-of-surface-plasmon-resonance-and-carbohydrate-recognition-domain-excision-mass-spectrometry
#6
Sira Defaus, Manuel Avilés, David Andreu, Ricardo Gutiérrez-Gallego
Seminal plasma proteins are relevant for sperm functionality and some appear responsible for establishing sperm interactions with the various environments along the female genital tract towards the oocyte. In recent years, research has focused on characterizing the role of these proteins in the context of reproductive biology, fertility diagnostics and treatment of related problems. Herein, we focus on the main protein of bovine seminal plasma, PDC-109 (BSP-A1/-A2), which by virtue of its lectin properties is involved in fertilization...
April 4, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29581386/siglec-expression-on-the-surface-of-human-bull-and-ram-sperm
#7
K Alkhodair, H Almhanna, J McGetrick, S Gedair, M E Gallagher, B Fernandez-Fuertes, T Tharmalingam, P B Larsen, E Fitzpatrick, P Lonergan, A C O Evans, S D Carrington, C J Reid
Sialic acid (Sia) is a major constituent of both the sperm glycocalyx and female reproductive mucosal surface and is involved in regulating sperm migration, uterotubal reservoir formation and oocyte binding. Siglecs (sialic acid-binding immunoglobulin - like lectins) commonly found on immune cells, bind to Sia in a linkage- and sugar-specific manner and often mediate cell-to-cell interactions and signalling. Proteomic and transcriptomic analysis of human and bovine sperm have listed Siglecs, but to date, their presence and/or localisation on sperm has not been studied...
April 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29580272/ejaculated-boar-spermatozoa-displaying-a-rare-multivesicular-defect
#8
Szabolcs Nagy, Tuire Tamminen, Magnus Andersson, Heriberto Rodriguez-Martinez
Two cases of a previously unreported sperm defect appearing in boar studs in Finland are presented. Spermatozoa showed small particles scattered on their surface with a prevalence decreasing with boar age. Semen samples, either stained with eosin-nigrosin or examined with phase contrast optics on formaldehyde-fixed spermatozoa, revealed the presence of multiple particles attached to the surface of spermatozoa counted as dead cells at fixation. Transmission electron microscopy revealed these were multivesicular and multilamellar vesicles, built up by phospholipid membranes...
March 27, 2018: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29567445/the-antidepressant-fluoxetine-alters-mechanisms-of-pre-and-post-copulatory-sexual-selection-in-the-eastern-mosquitofish-gambusia-holbrooki
#9
Michael G Bertram, Tiarne E Ecker, Bob B M Wong, Moira K O'Bryan, John B Baumgartner, Jake M Martin, Minna Saaristo
Contamination of aquatic habitats with pharmaceuticals is a major environmental concern. Recent studies have detected pharmaceutical pollutants in a wide array of ecosystems and organisms, with many of these contaminants being highly resistant to biodegradation and capable of eliciting sub-lethal effects in non-target species. One such pollutant is fluoxetine, a widely prescribed antidepressant, which is frequently detected in surface waters globally and can alter physiology and behaviour in aquatic organisms...
March 19, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29540542/plant-inspired-pipettes
#10
Keigo Nakamura, Tetsuya Hisanaga, Koichi Fujimoto, Keiji Nakajima, Hirofumi Wada
The female sex organ of the liverwort ( Marchantia polymorpha ) has a characteristic parasol-like form highly suitable for collecting water droplets containing sperm for fertilization. Motivated by this observation and using three-dimensional printing techniques, we develop a parasol-like rigid object that can grab, transport and release water droplets of a maximum size of about 1 cm. By combining experiments and scaling theory, we quantify the object's fundamental wetting and fluid dynamical properties. We construct a stability phase diagram and suggest that it is largely insensitive to properties of liquids such as surface tension and viscosity...
March 2018: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29512637/-effect-of-substitutions-in-surface-amino-acid-on-energy-profile-of-apomyoglobin
#11
M A Majorina, K A Glukhova, V V Marchenkov, B S Melnik
Studies on the process of spontaneous protein folding into a unique native state are an important issue of molecular biology. Apomyoglobin from the sperm whale is a convenient model for these studies in vitro. Here, we present the results of equilibrium and kinetic experiments carried out in a study on the folding and unfolding of eight mutant apomyoglobin forms of with hydrophobic amino acid substitutions on the protein surface. Calculated values of apparent constants of folding/unfolding rates, as well as the data on equilibrium conformational transitions in the urea concentration range of 0-6 М at 11°C are given...
January 2018: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/29482025/lifecycle-exposure-to-perchlorate-differentially-alters-morphology-biochemistry-and-transcription-as-well-as-sperm-motility-in-silurana-tropicalis-frogs
#12
Diana E K Campbell, Robert D Montgomerie, Valérie S Langlois
Perchlorate (ClO4 - ) contamination has been reported in ground and surface waters across North America. However, few studies have examined the effects of prolonged exposure to this thyroid hormone disrupting chemical, particularly at environmentally relevant concentrations in lower vertebrates, such as amphibians. The aim of this study was to examine the effects of a yearlong chronic exposure to ClO4 - in adult male and female Western clawed frogs (Silurana tropicalis). Frogs were spawned and raised from fertilized embryo until sexual maturity in potassium perchlorate (KClO4 )-treated water at different concentrations (0, 20, 53, and 107 μg/L)...
February 23, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29466382/mating-and-aggregative-behaviors-among-basal-hexapods-in-the-early-cretaceous
#13
Alba Sánchez-García, Enrique Peñalver, Xavier Delclòs, Michael S Engel
Among the many challenges in paleobiology is the inference and reconstruction of behaviors that rarely, if ever, leave a physical trace on the environment that is suitable for fossilization. Of particular significance are those behaviors tied to mating and courtship, individual interactions critical for species integrity and continuance, as well as those for dispersal, permitting the taxon to expand its distribution as well as access new habitats in the face of local or long-term environmental change. In this context, two recently discovered fossils from the Early Cretaceous amber of Spain (ca...
2018: PloS One
https://www.readbyqxmd.com/read/29448494/photocatalytic-degradation-of-oxytetracycline-by-ternary-mixed-catalyst-and-toxicity-assessment-using-boar-sperm
#14
Kefu Zhou, Xiao-Dan Xie, Xin-Yan Hu, Ming-Cheng Chen, Chang-Tang Chang
Response surface methodology was adopted to obtain ternary mixed catalysts of TiO2-loaded ZSM-5 zeolite and graphene. Oxytetracycline was used as challenged toxicant to evaluate the photocatalytic degradation efficiency of the composites. The optimal weight ratio of graphene, TiO2, and ZSM-5 was 1:8:1. The composites were characterized by ultraviolet-visible spectroscopy, X-ray diffraction, fourier transform infrared, N2 adsorption-desorption isotherms, and transmission electron microscope with an energy-dispersive spectroscopy system, etc...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29410678/actiflagelin-a-new-sperm-activator-isolated-from-walterinnesia-aegyptia-venom-using-phenotypic-screening
#15
Tarek Mohamed Abd El-Aziz, Sawsan Al Khoury, Lucie Jaquillard, Mathilde Triquigneaux, Guillaume Martinez, Sandrine Bourgoin-Voillard, Michel Sève, Christophe Arnoult, Rémy Beroud, Michel De Waard
Background: Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/29409138/treatment-of-semen-samples-with-%C3%AE-chymotrypsin-alters-the-expression-pattern-of-sperm-functional-proteins-a-pilot-study
#16
M K Panner Selvam, A Agarwal, R Sharma, L Samanta
Semen hyperviscosity delays the liquefaction of semen sample and is subjected to limited proteolysis by addition of α-chymotrypsin to reduce the viscosity. α-Chymotrypsin is a proteolytic enzyme involved in degradation of the proteins and polypeptides. Even though α-chymotrypsin improves the handling of hyperviscous samples, its effect on the sperm proteins is not clear. This study was aimed to evaluate the alteration in the expression of sperm functional proteins in samples treated with α-chymotrypsin...
February 6, 2018: Andrology
https://www.readbyqxmd.com/read/29385641/cellular-distribution-of-secretory-pathway-markers-in-the-haploid-synergid-cells-of-arabidopsis-thaliana
#17
Daniel S Jones, Xunliang Liu, Andrew C Willoughby, Benjamin E Smith, Ravishankar Palanivelu, Sharon A Kessler
In flowering plants, cell-cell communication plays a key role in reproductive success, as both pollination and fertilization require pathways that regulate interactions between many different cell types. Some of the most critical of these interactions are those between the pollen tube (PT) and the embryo sac, which ensure the delivery of the sperm cells required for double fertilization. Synergid cells function to attract the PT through the secretion of small peptides and in PT reception via membrane-bound proteins associated with the endomembrane system and the surface of the cell...
January 31, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29369695/integrin-heterodimers-expressed-on-the-surface-of-porcine-spermatogonial-stem-cells
#18
Min Hee Park, Min Seong Kim, Jung Im Yun, Jung Hoon Choi, Eunsong Lee, Seung Tae Lee
To date, in vitro culture systems able to sufficiently expand the small population of spermatogonial stem cells (SSCs), a tool for the development of sperm-mediated gene transfer techniques in transgenic pigs, in the porcine seminiferous tubule have not been reported. Therefore, as a step toward engineering a noncellular niche to support the in vitro maintenance of porcine SSC self-renewal, we investigated the types of integrin heterodimers that are expressed and functional on their membrane. The α and β integrin subunit protein expressions were analyzed using immunocytochemistry and fluorescence immunoassay, and the function of integrin heterodimers was confirmed by attachment and antibody inhibition assays...
January 25, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/29363381/clinical-outcomes-of-magnetic-activated-sperm-sorting-in-infertile-men-candidate-for-icsi
#19
Niloofar Ziarati, Marziyeh Tavalaee, Mehrnoosh Bahadorani, Mohammad Hossein Nasr Esfahani
Magnetic activated cell sorting (MACS) with annexin V microbeads deselected apoptotic sperm with externalized phosphatidylserine (PS) residues on their surface and decrease chance of such sperm to be inseminated. Therefore, the aim of this study was to evaluate efficiency of MACS procedure in a prospective randomized trial. Sixty-two semen samples were allocated into two groups and processed according to: (i) a combination of MACS with density gradient centrifugation (MACS-DGC) as study group (N = 29); and (ii) DGC alone as a control group (N = 33)...
January 24, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29358214/identification-of-jellyfish-neuropeptides-that-act-directly-as-oocyte-maturation-inducing-hormones
#20
Noriyo Takeda, Yota Kon, Gonzalo Quiroga Artigas, Pascal Lapébie, Carine Barreau, Osamu Koizumi, Takeo Kishimoto, Kazunori Tachibana, Evelyn Houliston, Ryusaku Deguchi
Oocyte meiotic maturation is crucial for sexually reproducing animals, and its core cytoplasmic regulators are highly conserved between species. By contrast, the few known maturation-inducing hormones (MIHs) that act on oocytes to initiate this process are highly variable in their molecular nature. Using the hydrozoan jellyfish species Clytia and Cladonema , which undergo oocyte maturation in response to dark-light and light-dark transitions, respectively, we deduced amidated tetrapeptide sequences from gonad transcriptome data and found that synthetic peptides could induce maturation of isolated oocytes at nanomolar concentrations...
January 22, 2018: Development
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