Samiya Rehman, Amjad Hussain, Munzer Ullah, Enas Ali, Mariusz Mojzych, Syed Ali Raza Naqvi, Akbar Ali, Meher Ali, Ehab Gomaa, Sherif S M Ghoneim, Bushra Mirza, Ken Keefover Ring, Hidayat Hussain, Abdur Rauf, Najeeb Ur Rehman, Faisal Attique
In ancient times, Withania coagulans Dunal was used as a therapeutic plant for the treatment of several diseases. This report aims to examine the effect of Agrobacterium tumefactions -mediated transformation of W. coagulans with the rolA gene to enhance secondary metabolite production, antioxidant activity, and anticancer activity of transformed tissues. Before transgenic plant production, the authors designed an efficient methodology for in vitro transformation. In this study, leaf explants were cultured on Murashage and Skoog (MS) media containing different amounts of naphthalene acetic acid (NAA) and benzyl adenine (BA)...
November 7, 2023: ACS Omega