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Shahrokh Ramin, Azad Sangin Abadi, Farideh Doroodgar, Mehrdad Esmaeili, Faizollah Niazi, Sana Niazi, Cyrus Alinia, Yaser Golestani, Reza Taj Abadi
This study was performed to evaluate the visual, refractive, and aberration measurement results of 2 implants, including Intacs Intracorneal Ring Segments (ICRS) and phakic Toric Implantable Collamer Lens (TICL), in patients with moderate Keratoconus (KCN). In this retrospective cross-sectional study, 30 patients with KCN with a mean age of 29.83 years were included in 2 groups, including the Intacs Intracorneal Ring Segments (ICRS) group and the phakic Toric Implantable Collamer Lens (TICL) group. Preoperative data as well as 6-month, 1-, 2-, 3- and 4-year follow-up data after the operation were collected and analyzed with the SPSS software (ver...
2018: Medical Hypothesis, Discovery and Innovation in Ophthalmology
Mitsuhiko Odera, Tadaomi Furuta, Yoshiro Sohma, Minoru Sakurai
Cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that belongs to the ATP binding cassette protein superfamily. Deletion of phenylalanine at position 508 (ΔF508) is the most common CF-associated mutation and is present in nearly 90% of CF patients. Currently, atomistic level studies are insufficient for understanding the mechanism by which the deletion of a single amino acid causes greatly reduced folding as well as trafficking and gating defects. To clarify this mechanism, we first constructed an atomic model of the inward-facing ΔF508-CFTR and performed allatom molecular dynamics (MD) simulations of the protein in a membrane environment...
2018: Biophysics and Physicobiology
Seung Woo Kim, Tae-Woong Um, Seunghoon Shin
Metal-free oxidation of ynamides is described, employing pyridine-N-oxides as oxidants under molecular iodine catalysis. In stark contrast to Brønsted acid catalysis, iodophilic activation of ynamides diverts the reaction manifold into a dioxygenation pathway. This oxidation is very rapid at room temperature with only 2.5 mol% of I2. Furthermore, this protocol could be extended to non-activated alkynes, such as diarylacetylenes, employing ICl as a catalyst to deliver benzil derivatives.
April 1, 2018: Journal of Organic Chemistry
Raymond Wong, Ahmed Abussaud, Joseph Wh Leung, Bao-Feng Xu, Fei-Ya Li, Sammen Huang, Nai-Hong Chen, Guan-Lei Wang, Zhong-Ping Feng, Hong-Shuo Sun
Activation of swelling-induced Cl- current (ICl,swell ) during neonatal hypoxia-ischemia (HI) may induce brain damage. Hypoxic-ischemic brain injury causes chronic neurological morbidity in neonates as well as acute mortality. In this study, we investigated the role of ICl,swell in hypoxic-ischemic brain injury using a selective blocker, 4-(2-butyl-6,7-dichloro-2-cyclopentylindan-1-on-5-yl) oxybutyric acid (DCPIB). In primary cultured cortical neurons perfusion of a 30% hypotonic solution activated ICl,swell , which was completely blocked by the application of DCPIB (10 μmol/L)...
March 29, 2018: Acta Pharmacologica Sinica
Jone Mitxelena, Aintzane Apraiz, Jon Vallejo-Rodríguez, Iraia García-Santisteban, Asier Fullaondo, Mónica Alvarez-Fernández, Marcos Malumbres, Ana M Zubiaga
The cellular response to DNA damage is essential for maintaining the integrity of the genome. Recent evidence has identified E2F7 as a key player in DNA damage-dependent transcriptional regulation of cell-cycle genes. However, the contribution of E2F7 to cellular responses upon genotoxic damage is still poorly defined. Here we show that E2F7 represses the expression of genes involved in the maintenance of genomic stability, both throughout the cell cycle and upon induction of DNA lesions that interfere with replication fork progression...
March 24, 2018: Nucleic Acids Research
Anita Kiss, Bianka Edina Herman, Tamás Görbe, Erzsébet Mernyák, Barnabás Molnár, János Wölfling, Mihály Szécsi, Gyula Schneider
The regioselective Cu(I)-catalyzed 1,3-dipolar cycloaddition of 17α- and 17β-azidoandrost-5-en-3β-ol epimers (3b and 5b) with different terminal alkynes afforded novel 1,4-substituted triazolyl derivatives (8a-k and 9a-k). For the preparation of 5'-iodo-1',2',3'-triazoles (8m-n and 9m-n), an improved method was developed, directly from steroidal azides and terminal alkynes, in reaction mediated by CuI and ICl as iodinating agents. Acetolysis and subsequent hydrolysis of 8n and 9n yielded 5'-hydroxy-1',2',3'-triazoles 8o and 9o...
March 22, 2018: Steroids
Muriel W Lambert
Nonerythroid αII-spectrin is a structural protein whose roles in the nucleus have just begun to be explored. αII-spectrin is an important component of the nucleoskelelton and has both structural and non-structural functions. Its best known role is in repair of DNA ICLs both in genomic and telomeric DNA. αII-spectrin aids in the recruitment of repair proteins to sites of damage and a proposed mechanism of action is presented. It interacts with a number of different groups of proteins in the nucleus, indicating it has roles in additional cellular functions...
March 2018: Experimental Biology and Medicine
Md Abu Sayeed, Kamrul Islam, Motaher Hossain, Noor Jahan Akter, Md Nur Alam, Nishat Sultana, Farhana Khanam, Meagan Kelly, Richelle C Charles, Pavol Kováč, Peng Xu, Jason R Andrews, Stephen B Calderwood, Jakia Amin, Edward T Ryan, Firdausi Qadri
Recognizing cholera cases early, especially in the initial phase of an outbreak and in areas where cholera has not previously circulated, is a high public health priority. Laboratory capacity in such settings is often limited. To address this, we have developed a rapid diagnostic test (RDT) termed Cholkit that is based on an immunochromatographic lateral flow assay for the diagnosis of cholera cases using stool. Cholkit contains a monoclonal antibody (ICL-33) to the O-specific polysaccharide (OSP) component of V...
March 14, 2018: PLoS Neglected Tropical Diseases
Zhihan Zhao, Huan Lan, Ibrahim El-Battrawy, Xin Li, Fanis Buljubasic, Katherine Sattler, Gökhan Yücel, Siegfried Lang, Malte Tiburcy, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Martin Borggrefe, Xiao-Bo Zhou, Ibrahim Akin
Background: Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery. However, the ion channel expression and functions as well as regulations in hiPSC-CMs still need to be fully characterized. Methods: Cardiomyocytes were derived from hiPS cells that were generated from two healthy donors. qPCR and patch clamp techniques were used for the study. Results: In addition to the reported ion channels, INa , ICa-L , ICa-T , If , INCX , IK1 , Ito , IKr , IKs IKATP , IK-pH , ISK1-3 , and ISK4 , we detected both the expression and currents of ACh-activated (KACh) and Na+ -activated (KNa) K+ , volume-regulated and calcium-activated (Cl-Ca) Cl- , and TRPV channels...
2018: Stem Cells International
Timsi Rao, Simonne Longerich, Weixing Zhao, Hideki Aihara, Patrick Sung, Yong Xiong
Fanconi-associated nuclease 1 (FAN1) removes interstrand DNA crosslinks (ICLs) through its DNA flap endonuclease and exonuclease activities. Crystal structures of human and bacterial FAN1 bound to a DNA flap have been solved. The Pseudomonas aeruginosa bacterial FAN1 and human FAN1 (hFAN1) missing a flexible loop are monomeric, while intact hFAN1 is homo-dimeric in structure. Importantly, the monomeric and dimeric forms of FAN1 exhibit very different DNA binding modes. Here, we interrogate the functional differences between monomeric and dimeric forms of FAN1 and provide an explanation for the discrepancy in oligomeric state between the two hFAN1 structures...
February 21, 2018: DNA Repair
Mihaela-Roxana Mănoiu, Jade Amine Amri, Alexandra Țicle, Cristina Stan
Optic nerve head drusen is an incidental finding in current ophthalmological practice. Although patients rarely display symptoms, structural, and functional defects, there are exceptional cases when clinical appearance can make it difficult to diagnose underlying or coexisting conditions, such as glaucoma. The following case report demonstrates how overlapping optic nerve pathologies can interfere in clinical judgement and therapeutic decision making in a young male patient, with relevant family history for both glaucoma and bilateral optic nerve head drusen...
October 2017: Romanian Journal of Ophthalmology
Hyeonseok Jin, Upasana Roy, Gwangrog Lee, Orlando D Schärer, Yunje Cho
DNA interstrand cross-links (ICLs) block the progress of the replication and transcription machineries and can weaken chromosomal stability, resulting in various diseases. FANCD2/FANCI-associated nuclease (FAN1) is a conserved structure-specific nuclease that unhooks DNA ICLs independently of the Fanconi anemia pathway. Recent structural studies have proposed two different mechanistic features for ICL unhooking by human FAN1: a specific basic pocket that recognizes the terminal phosphate of a 1-nt 5' flap or FAN1 dimerization...
March 7, 2018: Journal of Biological Chemistry
Magnus Liljenberg, Joakim Halldin Stenlid, Tore Brinck
The halogenation of monosubstituted benzenes in aqueous solvent has been studied using DFT at the PCM-M06-2X/6-311G(d,p) level. The reaction with Cl2 begins with the formation of C-atom coordinated π-complex and is followed by the formation of the σ-complex, which is rate-determining. The final part proceeds via the abstraction of the proton by a water molecule or a weak base. We evaluated the use of the σ-complex as a model for the rate-determining transition state (TS) and found that this model is more accurate the later the TS comes along the reaction coordinate...
March 5, 2018: Journal of Physical Chemistry. A
Chunguang Li, Lei Dong, Chuantao Zheng, Jun Lin, Yiding Wang, Frank K Tittel
A ppbv-level quartz-enhanced photoacoustic spectroscopy (QEPAS)-based ethane (C₂H₆) sensor was demonstrated by using a 3.3 μm continuous-wave (CW), distributed feedback (DFB) interband cascade laser (ICL). The ICL was employed for targeting a strong C₂H₆ absorption line located at 2996.88 cm-1 in its fundamental absorption band. Wavelength modulation spectroscopy (WMS) combined with the second harmonic (2f) detection technique was utilized to increase the signal-to-noise ratio (SNR) and simplify data acquisition and processing...
February 28, 2018: Sensors
Xiaohua Peng, Heli Fan, Huabing Sun
Recently, several aryl boronates have been reported to induce DNA interstrand cross-link (ICL) formation via carbocations upon photoirradiation. Herein we synthesized a series of new bifunctional phenyl compounds, in order to test the generality of such a mechanism, and understand how the chemical structure influences carbocation formation and DNA cross-linking process. These compounds efficiently form DNA ICLs via generated benzyl cations upon 350 nm irradiation. The DNA cross-linking efficiency and the pathway for carbocation generation depend on the aromatic substituents and the leaving groups...
February 25, 2018: Chemistry: a European Journal
Tarannum Mansoori, Satish Gooty Agraharam
PURPOSE: To report an interesting phenomenon, after vitreoretinal surgery, in an eye with toric implantable collamer lens (ICL). METHODS: A 20-year-old male presented with raised intraocular pressure (IOP) after the retinal detachment surgery in the left eye. He had toric ICL in both the eyes, and left eye showed shallow peripheral anterior chamber depth (ACD), narrow iridocorneal angle (ICA), increased ICL vault and pigments debris blocking central hole. After dilation, peripheral ACD deepened, ICL vault decreased and ICA increased, with posterior bowing of iris...
February 24, 2018: International Ophthalmology
Sunhee Ha, Bora Shin, Woojun Park
The aceA and glcB genes, encoding isocitrate lyase (ICL) and malate synthase, respectively, are not in an operon in many bacteria, including Pseudomonas aeruginosa, unlike in Escherichia coli. Here, we show that expression of aceA in P. aeruginosa is specifically upregulated under H2O2-induced oxidative stress and under iron-limiting conditions. In contrast, the addition of exogenous redox active compounds or antibiotics increases the expression of glcB. The transcriptional start sites of aceA under iron-limiting conditions and in the presence of iron were found to be identical by 5' RACE...
February 21, 2018: Microbiology
Mahmood Bagheri, Clifford Frez, Lukasz A Sterczewski, Ivan Gruidin, Mathieu Fradet, Igor Vurgaftman, Chadwick L Canedy, William W Bewley, Charles D Merritt, Chul Soo Kim, Mijin Kim, Jerry R Meyer
Since their inception, optical frequency combs have transformed a broad range of technical and scientific disciplines, spanning time keeping to navigation. Recently, dual comb spectroscopy has emerged as an attractive alternative to traditional Fourier transform spectroscopy, since it offers higher measurement sensitivity in a fraction of the time. Midwave infrared (mid-IR) frequency combs are especially promising as an effective means for probing the strong fundamental absorption lines of numerous chemical and biological agents...
February 20, 2018: Scientific Reports
ChunSik Choe, Johannes Schleusener, Jürgen Lademann, Maxim E Darvin
Porcine skin is widely used as a human skin model in dermatology. For both, porcine stratum corneum (SC) ex vivo and human SC in vivo, the hydrogen bonding states of water, the secondary and tertiary structures of keratin, the natural moisturizing factor (NMF) concentrations and the intercellular lipids' (ICL) lateral organization are investigated depth-dependently using confocal Raman microscopy. The SC depth profiles show that porcine SC ex vivo is characterized by lower hydrogen bonding states of water (10-30% SC depth), lower NMF concentration in the whole SC, more β-sheet form of keratin (10-90% SC depth), more folded tertiary keratin structures (30-70% SC depth) and higher hexagonal lateral packing order of ICL (10-50% SC depth) compared to human SC in vivo...
February 20, 2018: Journal of Biophotonics
Naved Akbar, Supriya Gupta, Apoorv Tiwari, K P Singh, Anil Kumar
In the present study, we identified seven major genes of oxalic acid biosynthesis pathway (SGAT, GGAT, ICL, GLO, MHAR, APO and OXO) from developing spike transcriptome of finger millet using rice as a reference. Sequence alignment of identified genes showed high similarity with their respective homolog in rice except for OXO and GLO. Transcript abundance (FPKM) reflects the higher accumulation of identified genes in GP-1 (low calcium genotype) as compared to GP-45 (high calcium genotype) which was further confirmed by qRT-PCR analysis, indicating differential oxalate formation in both genotypes...
April 5, 2018: Gene
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