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https://www.readbyqxmd.com/read/28317962/-phsio1-5-8-10-12-as-nanoreactors-for-non-enzymatic-introduction-of-ortho-meta-or-para-hydroxyl-groups-to-aromatic-molecules
#1
Mozhgan Bahrami, Xingwen Zhang, Morteza Ehsani, Yousef Jahani, Richard M Laine
Traditional electrophilic bromination follows long established "rules": electron-withdrawing substituents cause bromination selective for meta positions, whereas electron-donating substituents favor ortho and para bromination. In contrast, in the [PhSiO1.5]8,10,12 silsesquioxanes, the cages act as bulky, electron withdrawing groups equivalent to CF3; yet bromination under mild conditions, without a catalyst, greatly favors ortho substitution. Surprisingly, ICl iodination without a catalyst favors (>90%) para substitution [p-IC6H4SiO1...
March 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28292785/recruitment-and-positioning-determine-the-specific-role-of-the-xpf-ercc1-endonuclease-in-interstrand-crosslink-repair
#2
Daisy Klein Douwel, Wouter S Hoogenboom, Rick Acm Boonen, Puck Knipscheer
XPF-ERCC1 is a structure-specific endonuclease pivotal for several DNA repair pathways and, when mutated, can cause multiple diseases. Although the disease-specific mutations are thought to affect different DNA repair pathways, the molecular basis for this is unknown. Here we examine the function of XPF-ERCC1 in DNA interstrand crosslink (ICL) repair. We used Xenopus egg extracts to measure both ICL and nucleotide excision repair, and we identified mutations that are specifically defective in ICL repair. One of these separation-of-function mutations resides in the helicase-like domain of XPF and disrupts binding to SLX4 and recruitment to the ICL A small deletion in the same domain supports recruitment of XPF to the ICL, but inhibited the unhooking incisions most likely by disrupting a second, transient interaction with SLX4...
March 14, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28292776/comparison-of-corneal-endothelial-cell-density-and-morphology-after-posterior-chamber-phakic-intraocular-lens-implantation-with-and-without-a-central-hole
#3
Hiroyasu Goukon, Kazutaka Kamiya, Kimiya Shimizu, Akihito Igarashi
AIM: To compare the corneal endothelial cells after posterior chamber phakic intraocular lens with and without a central hole (hole implantable collamer lens (ICL) and conventional ICL) implantation for the correction of moderate to high myopia. METHODS: This retrospective study evaluated 34 eyes of 34 patients who underwent hole ICL implantation and 25 eyes of 25 patients who underwent conventional ICL implantation. Preoperatively and 3 months, and 1 and 2 years postoperatively, we compared the central corneal endothelial cell density (ECD), coefficient of variation in cell size (CV) and the percentage of hexagonal cells (HEX) between the two groups using a non-contact specular microscope (EM-3000, Tomey)...
March 14, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28291554/implantation-of-posterior-chamber-phakic-intraocular-lens-for-myopia-and-hyperopia%C3%A2-%C3%A2-long-term-clinical-outcomes
#4
H Kocová, E Vlková, L Michalcová, O Motyka
PURPOSE: To evaluate long-term refractive outcomes of implantable collamer lens (ICL) implantation and late postoperative complications. METHODS: We assessed outcomes of patients who underwent ICL implantation (type ICM V4 for myopia, ICH V3 for hyperopia, TICM V4 for astigmatism) in our department between 1998 and 2013. It comprised 62 eyes (40 myopic and 22 hyperopic). The average follow-up period was 10.5 years. We evaluated: uncorrected and best-corrected visual acuity (UCVA and BCVA), spherical equivalent (SE), ICL vault, endothelial cell density and late postoperative complications...
March 10, 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28291357/self-assembling-behavior-of-glycerol-monoundecenoate-in-water
#5
Gildas Nyame Mendendy Boussambe, Romain Valentin, Jean-François Fabre, Laurence Navailles, Frédéric Nallet, Cedric Gaillard, Zephirin Mouloungui
Self-assembling properties of glycerol esters in water are well known. Still, few data on glyc-erol monoesters of undecylenic acid are available. The aim of this study was to highlight the behavior of the glycerol monoundecenoate (GM-C11:1) in different, diluted and concentrated states. Self-assembling properties in water and upon solid inorganic surfaces were investigated in diluted state with surface tension experiments, AFM and Cryo-TEM studies. In concen-trated state, the gelling properties in presence of water were investigated by polarized light microscopy, DSC and SAXS experiments...
March 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28265055/glyoxylate-detoxification-is-an-essential-function-of-malate-synthase-required-for-carbon-assimilation-in-mycobacterium-tuberculosis
#6
Susan Puckett, Carolina Trujillo, Zhe Wang, Hyungjin Eoh, Thomas R Ioerger, Inna Krieger, James Sacchettini, Dirk Schnappinger, Kyu Y Rhee, Sabine Ehrt
The glyoxylate shunt is a metabolic pathway of bacteria, fungi, and plants used to assimilate even-chain fatty acids (FAs) and has been implicated in persistence of Mycobacterium tuberculosis (Mtb). Recent work, however, showed that the first enzyme of the glyoxylate shunt, isocitrate lyase (ICL), may mediate survival of Mtb during the acute and chronic phases of infection in mice through physiologic functions apart from fatty acid metabolism. Here, we report that malate synthase (MS), the second enzyme of the glyoxylate shunt, is essential for in vitro growth and survival of Mtb on even-chain fatty acids, in part, for a previously unrecognized activity: mitigating the toxicity of glyoxylate excess arising from metabolism of even-chain fatty acids...
March 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28262948/swelling-induced-chloride-current-in-glioblastoma-proliferation-migration-and-invasion
#7
Raymond Wong, Wenliang Chen, Xiao Zhong, James T Rutka, Zhong-Ping Feng, Hong-Shuo Sun
Glioblastoma (GBM) remains as the most common and aggressive brain tumor. The survival of GBM has been linked to the aberrant activation of swelling-induced chloride current ICl,swell . In this study, we investigated the effects of ICl,swell on cell viability, proliferation and migration in the human GBM cell lines, U251 and U87, using a combination of patch clamp electrophysiology, MTT, colony formation, wound healing assays and Western immunoblotting. First, we showed that the specific inhibitor of ICl,swell , DCPIB, potently reduced the ICl,swell in U87 cells...
March 6, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28212311/detection-of-atmospheric-methyl-mercaptan-using-wavelength-modulation-spectroscopy-with-multicomponent-spectral-fitting
#8
Zhenhui Du, Jiaxin Wan, Jinyi Li, Gang Luo, Hong Gao, Yiwen Ma
Detection of methyl mercaptan (CH₃SH) is essential for environmental atmosphere assessment and exhaled-breath analysis. This paper presents a sensitive CH₃SH sensor based on wavelength modulation spectroscopy (WMS) with a mid-infrared distributed feedback interband cascade laser (DFB-ICL). Multicomponent spectral fitting was used not only to enhance the sensitivity of the sensor but also to determine the concentration of interferents (atmospheric water and methane). The results showed that the uncertainties in the measurement of CH₃SH, H₂O, and CH₄ were less than 1...
February 16, 2017: Sensors
https://www.readbyqxmd.com/read/28205338/assessing-oxygen-saturation-in-retinal-vessels-in-high-myopia-patients-pre-and-post-implantable-collamer-lens-implantation-surgery
#9
Pei Chen, Xiaoxiao Cai, Lijun Xu, Jing Zhang, Ying Yang, Qianying Gao, Jian Ge, Keming Yu, Jing Zhuang
PURPOSE: To determine whether posterior chamber phakic implantable collamer lens (ICL) surgery in high myopia patients impedes oxygen saturation of retinal vessels. METHODS: Mean oxygen saturation and diameter in retinal blood vessels were measured before and after ICL implantation surgery to correct high myopia refractive errors (i.e. -6.00 to -20.25 dioptres [D]), using an Oxymap T1 retinal oximeter. RESULTS: In 17 eyes of 17 patients, the Oxymap T1 retinal oximeter detected a small but significant decrease in oxygen saturation of retinal venules, 1-week postoperatively (compared to preoperative measurements)...
February 15, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28203053/refractive-outcomes-and-safety-of-the-implantable-collamer-lens-in-young-low-to-moderate-myopes
#10
Paul J Dougherty, Taylor Priver
PURPOSE: To evaluate visual and refractive outcomes and safety of implantable collamer lens (ICL) implantation in low-to-moderate myopia. SETTING: Private Practice - Dougherty Laser Vision, Westlake Village, CA, USA. METHODS: A chart review was performed in 56 (104 eyes) consecutive patients who underwent implantation of the Visian ICL V4 model. Inclusion criteria were age between 21 and 40 years, spherical equivalent between -3.00 D and -10...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28197307/synthesis-and-antitumor-activity-evaluation-of-a-novel-combi-nitrosourea-prodrug-bgcnu
#11
Yameng Wang, Ting Ren, Xinxin Lai, Guohui Sun, Lijiao Zhao, Na Zhang, Rugang Zhong
Chloroethylnitrosoureas (CENUs) are an important type of alkylating agent employed in the clinical treatment of cancer. However, the anticancer efficacy of CENUs is greatly decreased by a DNA repairing enzyme, O(6)-alkylguanine-DNA alkyltransferase (AGT), by preventing the formation of interstrand cross-links (ICLs). In this study, a combi-nitrosourea prodrug, namely, N-(2-chloroethyl)-N'-2-(O(6)-benzyl-9-guanine)ethyl-N-nitrosourea (BGCNU), which possesses an O(6)-benzylguanine (O(6)-BG) derivative and CENU pharmacophores simultaneously, was synthesized and evaluated for its ability to induce ICLs...
February 9, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28178691/p-glycoprotein-attenuates-dna-repair-activity-in-multidrug-resistant-cells-by-acting-through-the-cbp-csk-src-cascade
#12
Li-Fang Lin, Ming-Hsi Wu, Vijaya Kumar Pidugu, I-Ching Ho, Tsann-Long Su, Te-Chang Lee
Recent studies have demonstrated that P-glycoprotein (P-gp) expression impairs DNA interstrand cross-linking agent-induced DNA repair efficiency in multidrug-resistant (MDR) cells. To date, the detailed molecular mechanisms underlying how P-gp interferes with Src activation and subsequent DNA repair activity remain unclear. In this study, we determined that the C-terminal Src kinase-binding protein (Cbp) signaling pathway involved in the negative control of Src activation is enhanced in MDR cells. We also demonstrated that cells that ectopically express P-gp exhibit reduced activation of DNA damage response regulators, such as ATM, Chk2, Braca1 and Nbs1 and hence attenuated DNA double-strand break repair capacity and become more susceptible than vector control cells to DNA interstrand cross-linking (ICL) agents...
February 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28171776/adverse-events-associated-with-the-use-of-cervical-spine-manipulation-or-mobilization-and-patient-characteristics-a-systematic-review
#13
REVIEW
H A Kranenburg, M A Schmitt, E J Puentedura, G J Luijckx, C P van der Schans
Cervical spinal manipulation (CSM) and cervical mobilization are frequently used in patients with neck pain and headache. Pre-manipulative cervical instability and arterial integrity tests appear to be unreliable in identifying patients at risk for adverse events. It would be valuable if patients at risk could be identified by specific characteristics during the preliminary screening. Objective was to identify characteristics of 1) patients, 2) practitioners, 3) treatment process and 4) adverse events (AE) occurring after CSM or cervical mobilization...
April 2017: Musculoskelet Sci Pract
https://www.readbyqxmd.com/read/28151460/advancing-nursing-practice-the-emergence-of-the-role-of-advanced-practice-nurse-in-saudi-arabia
#14
Denise Hibbert, Ahmad E Aboshaiqah, Kathy A Sienko, Debra Forestell, Adel W Harb, Shadia A Yousuf, Patricia W Kelley, Patricia F Brennan, Laura Serrant, Alison Leary
BACKGROUND: The roots of advanced practice nursing (APN) can be traced back to the 1890s, but the nurse practitioner (NP) emerged in Western countries during the 1960s in response to the unmet healthcare needs of populations in rural areas. These early NPs utilized the medical model of care to assess, diagnose and treat. Nursing has since grown as a profession, with its own unique and distinguishable, holistic, science-based knowledge, which is complementary within the multidisciplinary team...
January 2017: Annals of Saudi Medicine
https://www.readbyqxmd.com/read/28143714/chloroethylating-nitrosoureas-in-cancer-therapy-dna-damage-repair-and-cell-death-signaling
#15
REVIEW
Teodora Nikolova, Wynand P Roos, Oliver H Krämer, Herwig M Strik, Bernd Kaina
Chloroethylating nitrosoureas (CNU), such as lomustine, nimustine, semustine, carmustine and fotemustine are used for the treatment of malignant gliomas, brain metastases of different origin, melanomas and Hodgkin disease. They alkylate the DNA bases and give rise to the formation of monoadducts and subsequently interstrand crosslinks (ICL). ICL are critical cytotoxic DNA lesions that link the DNA strands covalently and block DNA replication and transcription. As a result, S phase progression is inhibited and cells are triggered to undergo apoptosis and necrosis, which both contribute to the effectiveness of CNU-based cancer therapy...
January 29, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28130555/xpf-knockout-via-crispr-cas9-reveals-that-ercc1-is-retained-in-the-cytoplasm-without-its-heterodimer-partner-xpf
#16
Janin Lehmann, Christina Seebode, Sabine Smolorz, Steffen Schubert, Steffen Emmert
The XPF/ERCC1 heterodimeric complex is essentially involved in nucleotide excision repair (NER), interstrand crosslink (ICL), and double-strand break repair. Defects in XPF lead to severe diseases like xeroderma pigmentosum (XP). Up until now, XP-F patient cells have been utilized for functional analyses. Due to the multiple roles of the XPF/ERCC1 complex, these patient cells retain at least one full-length allele and residual repair capabilities. Despite the essential function of the XPF/ERCC1 complex for the human organism, we successfully generated a viable immortalised human XPF knockout cell line with complete loss of XPF using the CRISPR/Cas9 technique in fetal lung fibroblasts (MRC5Vi cells)...
January 27, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28125837/regulatory-roles-of-anoctamin-6-in-human-trabecular-meshwork-cells
#17
Juni Banerjee, Chi-Ting Leung, Ang Li, Kim Peterson-Yantorno, Huan Ouyang, W Daniel Stamer, Mortimer M Civan
Purpose: Trabecular meshwork (TM) cell volume is a determinant of aqueous humor outflow resistance, and thereby IOP. Regulation of TM cell volume depends on chloride ion (Cl-) release through swelling-activated channels (ICl,Swell), whose pore is formed by LRRC8 proteins. Chloride ion release through swelling-activated channels has been reported to be regulated by calcium-activated anoctamins, but this finding is controversial. Particularly uncertain has been the effect of anoctamin Ano6, reported as a Ca2+-activated Cl- (CaCC) or cation channel in other cells...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28120709/dna-damaging-anticancer-drugs-a-perspective-for-dna-repair-oriented-therapy
#18
Janusz Blasiak
DNA-damaging drugs in cancer present two main problems: therapeutic resistance and side effects and both can associate with DNA repair, which can be targeted in cancer therapy. Bleomycin (BLM) induces complex DNA damages, including strand breaks, base loss and 3'-phosphoglycolate (3'PG) residues repaired by several pathways, but 3'PGs must be processed to the 3'-OH ends, usually by tyrosyl-DNA phosphodiesterase 1 (Tdp1). Therefore, targeting Tdp1 can improve anticancer therapy with BLM. Mitomycin C (MMC) produces a variety of adducts with DNA, including inter-strand cross-links (ICLs) and Xeroderma pigmentosum (XP) proteins, including XPG, XPE and XPF can be crucial for the initial stage of ICL repair, so they can be targeted by inhibitors to increase toxicity of MMC in cancer cells...
January 24, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28110804/differential-role-of-base-excision-repair-proteins-in-mediating-cisplatin-cytotoxicity
#19
Akshada Sawant, Ashley M Floyd, Mohan Dangeti, Wen Lei, Robert W Sobol, Steve M Patrick
Interstrand crosslinks (ICLs) are covalent lesions formed by cisplatin. The mechanism for the processing and removal of ICLs by DNA repair proteins involves nucleotide excision repair (NER), homologous recombination (HR) and fanconi anemia (FA) pathways. In this report, we monitored the processing of a flanking uracil adjacent to a cisplatin ICL by the proteins involved in the base excision repair (BER) pathway. Using a combination of extracts, purified proteins, inhibitors, functional assays and cell culture studies, we determined the specific BER proteins required for processing a DNA substrate with a uracil adjacent to a cisplatin ICL...
March 2017: DNA Repair
https://www.readbyqxmd.com/read/28106892/theoretical-study-on-the-need-for-laser-iridotomy-in-an-implantable-collamer-lens-with-a-hole-using-computational-fluid-dynamics
#20
T Kawamorita, K Shimizu, N Shoji
PurposeAlthough one of the advantages of the Hole-ICL implantation is that laser iridotomy (LI) is unnecessary, the evidence have not been reported from the viewpoint of aqueous humor circulation. We investigated the effect of laser iridotomy (LI) on the fluid dynamics of aqueous humor in an implantable collamer lens (ICL) with a central hole, that is, a Hole-ICL using computational fluid dynamics.MethodsA fluid dynamics simulation was performed using the thermal-hydraulic analysis software FloEFD (Mentor Graphics Corp...
January 20, 2017: Eye
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