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Can-Zhao Liu, Xiang-Yu Li, Ren-Hong Du, Min Gao, Ming-Ming Ma, Fei-Ya Li, Er-Wen Huang, Hong-Shuo Sun, Guan-Lei Wang, Yong-Yuan Guan
BACKGROUND: Previous research has demonstrated that ClC-3 is responsible for volume-regulated Cl(-)current (ICl.vol) in vascular smooth muscle cells (VSMCs). However, it is still not clear whether and how ClC-3 is transported to cell membranes, resulting in alteration ofICl.vol.Methods and Results:Volume-regulated chloride current (ICl.vol) was recorded by whole-cell patch clamp recording, and Western blotting and co-immunoprecipitation were performed to examine protein expression and protein-protein interaction...
October 19, 2016: Circulation Journal: Official Journal of the Japanese Circulation Society
Qiuyi Zhang, Xuchao Zhang, Honghong Yan, Benyuan Jiang, Chongrui Xu, Jinji Yang, Zhihong Chen, Jian Su, Yi-Long Wu, Qing Zhou
BACKGROUND: Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are remarkably effective for treating EGFR-mutant non-small cell lung cancer (NSCLC). However, the individual role of EGFR-TKIs in patients with brain metastasis (BM) arising from EGFR-mutant NSCLC remains unclear. METHODS: Patients with BM secondary to NSCLC and harboring EGFR-activating mutations were retrospectively screened. Patients who received gefitinib or erlotinib to control both extracranial lesions (ECLs) and intracranial lesions (ICLs) were eligible...
September 1, 2016: Thoracic Cancer
Wynand Paul Roos, Andrea Krumm
Histone/protein deacetylases play multiple roles in regulating gene expression and protein activation and stability. Their deregulation during cancer initiation and progression cause resistance to therapy. Here, we review the role of histone deacetylases (HDACs) and the NAD(+) dependent sirtuins (SIRTs) in the DNA damage response (DDR). These lysine deacetylases contribute to DNA repair by base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), non-homologous end joining (NHEJ), homologous recombination (HR) and interstrand crosslink (ICL) repair...
October 13, 2016: Nucleic Acids Research
P K Ramalingam, K Gayathri, Rpsp Santhakumar, B V Manjunath, N Karuppuswamy, B Vetriveeran, S Selvamani, P Vishnuram, Kumar Natarajan
We report a 42 year old male who was an interesting case of "idiopathic CD4 lymphocytopenia"(ICL) in a non-HIV adult with-extra pulmonary tuberculosis along with the diffuse splenic calcification and whose symptoms improved with Antitubercular treatment. He was found to have low CD4 counts on two occasions.
May 2016: Journal of the Association of Physicians of India
Elisa Gabanti, Francesca Bruno, Lucia Scaramuzzi, Filippo Mangione, Paola Zelini, Giuseppe Gerna, Daniele Lilleri
Human cytomegalovirus (HCMV) is still the most common viral infection in solid-organ transplant recipients (SOTR). Our study aimed to identify the predictive values of the T-cell response able to protect from HCMV disease, according to different assays. Viral DNA was determined by real-time PCR. The T-cell immune response to HCMV infection was investigated in SOTR according to the following assays and stimuli: cytokine flow cytometry (CFC) after peripheral blood mononuclear cell (PBMC) stimulation with autologous HCMV-infected dendritic cells (iDC) vs three ELISPOT assays using PBMCs stimulated with: i) HCMV-infected cell lysate (iCL); ii) a pool of 34 epitopic peptides (PP) from different HCMV proteins; iii) a commercial pp65 peptide pool (CPM)...
September 13, 2016: New Microbiologica
Magdalena Zdrowowicz, Paweł Wityk, Barbara Michalska, Janusz Rak
A double-stranded oligonucleotide, 80 base pairs in length, was multiply labeled with 5-bromo-2'-deoxycytidine (BrdC) using polymerase chain reaction (PCR). The modified oligonucleotide was irradiated with 300 nm photons and its damage was assayed by employing DHPLC, LC-MS and denaturing polyacrylamide gel electrophoresis (PAGE). Two types of damage were demonstrated, namely, single strand breaks (SSBs) and intrastrand cross-links (ICLs); the ICLs were in the form of d(G^C) and d(C^C) dimers. The former species are probably formed due to photoinduced electron transfer between the photoexcited BrdC and the ground state 2'-deoxyguanosine (dG), whereas the latter is a result of a cycloaddition reaction...
October 4, 2016: Organic & Biomolecular Chemistry
Daniel R Semlow, Jieqiong Zhang, Magda Budzowska, Alexander C Drohat, Johannes C Walter
During eukaryotic DNA interstrand cross-link (ICL) repair, cross-links are resolved ("unhooked") by nucleolytic incisions surrounding the lesion. In vertebrates, ICL repair is triggered when replication forks collide with the lesion, leading to FANCI-FANCD2-dependent unhooking and formation of a double-strand break (DSB) intermediate. Using Xenopus egg extracts, we describe here a replication-coupled ICL repair pathway that does not require incisions or FANCI-FANCD2. Instead, the ICL is unhooked when one of the two N-glycosyl bonds forming the cross-link is cleaved by the DNA glycosylase NEIL3...
October 6, 2016: Cell
Mohd Quadir Siddiqui, Rajan Kumar Choudhary, Pankaj Thapa, Neha Kulkarni, Yogendra S Rajpurohit, Hari S Misra, Nikhil Gadewal, Satish Kumar, Syed K Hasan, Ashok K Varma
Fanconi anemia complementation groups - I (FANCI) protein facilitates DNA ICL (Inter-Cross-link) repair and plays a crucial role in genomic integrity. FANCI is a 1328 amino acids protein which contains armadillo (ARM) repeats and EDGE motif at the C-terminus. ARM repeats are functionally diverse and evolutionarily conserved domain that plays a pivotal role in protein-protein and protein-DNA interactions. Considering the importance of ARM repeats, we have explored comprehensive in-silico and in-vitro approach to examine folding pattern...
September 30, 2016: Journal of Biomolecular Structure & Dynamics
Sherif A Eissa
PURPOSE: To assess the outcomes of sulcus implantation of the Visian(®) implantable collamer lens (ICL) to correct pseudophakic myopic anisometropic amblyopia with myopic shift and/or primary refractive overcorrection. METHODS: Prospective case series enrolled 14 pseudophakic eyes of 14 patients, 5-9 years old, with history of cataract surgery and primary in the bag-intraocular lenses (IOL) implantation, followed by myopic shift and/or refractive overcorrection and anisometropic amblyopia of variable degrees...
September 29, 2016: Acta Ophthalmologica
Youngsub Eom, Dae Wook Kim, Dongok Ryu, Jun-Heon Kim, Seul Ki Yang, Jong Suk Song, Sug-Whan Kim, Hyo Myung Kim
PURPOSE: To evaluate the incidence of central hole-induced ring-shaped dysphotopsia after posterior chamber phakic implantable collamer lens (ICL) with central hole (hole ICL) implantation and to investigate the causes of central hole-induced dysphotopsia. METHODS: The clinical study enrolled 29 eyes of 15 consecutive myopic patients implanted with hole ICL. The incidence of ring-shaped dysphotopsia after hole ICL implantation was evaluated. In the experimental simulation study, non-sequential ray tracing was used to construct myopic human eye models with hole ICL and ICL without a central hole (conventional ICL)...
September 28, 2016: Acta Ophthalmologica
Houda Migaou Miled, Hichem Ben Brahim, Yafa Hadj Hassine, Soumaya Boudokhane, Aymen Haj Salah, Nesrine Abdelkafi, Sana Salah, Anis Jellad, Zohra Ben Salah Frih
OBJECTIVE: The objective of this study was to assess the functional and socio-professional future of lower limb-amputated patients. PATIENTS AND METHODS: This is a 3-year-prospective study of lower limb-amputated patients, followed at the Physical Medicine and Rehabilitation department of the University Hospital of Monastir. The data analyzed were epidemiological, clinical, functional [the index of locomotion (ICL), the score "Special interest group of the amputee physician" (SIGAM)] and socio-professionals...
September 2016: Annals of Physical and Rehabilitation Medicine
Houda Migaou Miled, Hichem Ben Brahim, Yafa Hadj Hassine, Soumaya Boudokhane, Nesrine Abdelkafi, Aymen Haj Salah, Sana Salah, Anis Jellad, Zohra Ben Salah Frih
OBJECTIVE: Assess satisfaction of lower limb amputees towards their device. PATIENTS AND METHODS: Prospective study of device-fitted lower limb amputees followed at the Physical Medicine and Rehabilitation department at the University Hospital of Monastir. Epidemiological and clinical parameters were assessed with evaluation of fitted patients' satisfaction towards their prosthesis (survey on the satisfaction of patients with lower limb amputations towards their prosthesis) (SAT-PRO)...
September 2016: Annals of Physical and Rehabilitation Medicine
Ahmed Mohamed Kamal Elshafei, Mahmoud Mohamed Genaidy, Hossam Mohamed Moharram
Purpose. To evaluate the anterior segment, the anatomical position of the implantable collamer lenses (ICL), and its relationship to adjacent ocular structures using Ultrasound Biomicroscopy (UBM). Methods. In a prospective study, 142 myopic eyes of 93 patients implanted with Visian ICL were subjected to UBM examination between March 2010 and January 2015. The relative position of ICL to the adjacent structure and the overall iris configuration were evaluated. The machine calibers were used to measure the minimum central distance between the ICL and anterior lens capsule (vault) and the vertical central distance between the corneal endothelium and the ICL (E-ICL)...
2016: Journal of Ophthalmology
Shu-Yuan Cheng, Jiwon Seo, Bik Tzu Huang, Tanya Napolitano, Elise Champeil
Mitomycin C (MC), a commonly used anticancer drug, induces DNA damage via DNA alkylation. Decarbamoyl mitomycin C (DMC), another mitomycin lacking the carbamate at C10, generates similar lesions as MC. Interstrand cross-links (ICLs) are believed to be the lesions primarily responsible for the cytotoxicity of MC and DMC. The major ICL generated by MC (α-ICL) has a trans stereochemistry at the guanine-drug linkage whereas the major ICL from DMC (β-ICL) has the opposite, cis, stereochemistry. In addition, DMC can provoke strong p53-independent cell death...
September 23, 2016: International Journal of Oncology
Xinfang Cao, Weiliang Wu, Yang Wang, Chen Xie, Ye Shen
PURPOSE: To compare the longitudinal vault changes after implantation of a posterior chamber phakic intraocular lens (pIOL) (Visian Implantable Contact Lens) with (ICL V4c) and without (ICL V4) a central artificial hole for moderate to high myopia in Chinese eyes. DESIGN: Retrospective case series. METHODS: This study comprised 78 eyes implanted with the ICL V4c model and 82 eyes implanted with the ICL V4 model at our department by the same surgeon...
September 19, 2016: American Journal of Ophthalmology
George Fullbright, Halley B Rycenga, Jordon D Gruber, David T Long
Inter-strand crosslinks (ICLs) are extremely toxic DNA lesions that create an impassable roadblock to DNA replication. When a replication fork collides with an ICL, it triggers a damage response that promotes multiple DNA processing events required to excise the crosslink from chromatin and resolve the stalled replication fork. One of the first steps in this process involves displacement of the CMG replicative helicase (comprised of Cdc45, MCM2-7, and GINS), which obstructs the underlying crosslink. Here we report that the p97/Cdc48/VCP segregase plays a critical role in ICL repair by unloading the CMG complex from chromatin...
September 19, 2016: Molecular and Cellular Biology
Spyridon Varthis, Anthony Randi, Dennis P Tarnow
PURPOSE: The aim of this study was to determine the prevalence of interproximal open contacts between singleimplant prostheses and adjacent teeth, as well as to provide guidelines to prevent interproximal contact loss (ICL). MATERIALS AND METHODS: This was a retrospective, cross-sectional study. One hundred twenty-eight patients (174 single-implant restorations) from Columbia University College of Dental Medicine and a private faculty clinic with a single-implant restoration in the posterior or anterior region were selected to participate in this study...
September 2016: International Journal of Oral & Maxillofacial Implants
Xun Chen, Huamao Miao, Rajeev Krishnan Naidu, Xiaoying Wang, Xingtao Zhou
BACKGROUND: To objectively compare the early changes in vault over time following implantation of an Implantable Collamer Lens without (ICL V4) and with (ICL V4c) a central hole and the respective factors affecting vault change in moderate to high myopia. METHODS: This prospective study comprised of 38 eyes of 38 patients implanted with ICL V4 and 39 eyes of 39 patients implanted with ICL V4c intraocular lenses. We quantitatively assessed the postoperative values of vault and pupil size at 1 day, 1 week, and 1 month following implantation using a rotating Scheimpflug camera (Pentacam)...
2016: BMC Ophthalmology
José Ignacio Fernández-Vigo, Ana Macarro-Merino, Joaquín Fernández-Francos, Lucía De-Pablo-Gómez-de-Liaño, José María Martínez-de-la-Casa, Julián García-Feijóo, José Ángel Fernández-Vigo
PURPOSE: To compare the behavior of aqueous humor (AH) and analyze flow differences by comparing the volume and velocity of the flow after two different models of implantable collamer lens (ICL) placement. METHODS: Computational fluid dynamics with numerical simulation using Ansys Fluent software was performed to compare the AH flow through a peripheral iridotomy (PI), which is typically performed after implantation of a V4b lens to the central hole of a V4c lens...
September 1, 2016: Investigative Ophthalmology & Visual Science
Xiao Zhang, Xiang-Chen Tao, Zhi-Wei Li, Wei-Yan Zhou, Ping Ma, Chun-Xiao Zhang, Yu-Meng Wang, Guo-Ying Mu
The present study reports the use of a phakic toric Implantable Collamer Lens (ICL) that improved the refraction correction of high myopia and astigmatism in a case of keratectasia following corneal cross-linking. A 31-year-old male was diagnosed with keratectasia 12 years after laser-assisted in situ keratomileusis (LASIK). Following LASIK, the manifest refraction was -3.50-2.25×90 [0.1 logarithmic expression (LogMAR) best corrected visual acuity (BVCA)] in the right eye and -8.00-3.50×175 (0.3 LogMAR BCVA) in the left eye, with a LogMAR uncorrected distance visual acuity (UDVA) of 0...
September 2016: Experimental and Therapeutic Medicine
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