keyword
MENU ▼
Read by QxMD icon Read
search

tracking genetically engineered T cells

keyword
https://www.readbyqxmd.com/read/27486853/oncolytic-virus-therapy-a-new-era-of-cancer-treatment-at-dawn
#1
REVIEW
Hiroshi Fukuhara, Yasushi Ino, Tomoki Todo
Oncolytic virus therapy is perhaps the next major breakthrough in cancer treatment following the success in immunotherapy using immune checkpoint inhibitors. Oncolytic viruses are defined as genetically engineered or naturally occurring viruses that selectively replicate in and kill cancer cells without harming the normal tissues. T-Vec (talimogene laherparepvec), a second-generation oncolytic herpes simplex virus type 1 (HSV-1) armed with GM-CSF, was recently approved as the first oncolytic virus drug in the USA and Europe...
October 2016: Cancer Science
https://www.readbyqxmd.com/read/26900664/inclusion-of-strep-tag-ii-in-design-of-antigen-receptors-for-t-cell-immunotherapy
#2
Lingfeng Liu, Daniel Sommermeyer, Alexandra Cabanov, Paula Kosasih, Tyler Hill, Stanley R Riddell
Adoptive immunotherapy with genetically engineered T cells has the potential to treat cancer and other diseases. The introduction of Strep-tag II sequences into specific sites in synthetic chimeric antigen receptors or natural T-cell receptors of diverse specificities provides engineered T cells with a marker for identification and rapid purification, a method for tailoring spacer length of chimeric receptors for optimal function, and a functional element for selective antibody-coated, microbead-driven, large-scale expansion...
April 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/26830315/studying-platinum-sensitivity-and-resistance-in-high-grade-serous-ovarian-cancer-different-models-for-different-questions
#3
REVIEW
Nicolette G Alkema, G Bea A Wisman, Ate G J van der Zee, Marcel A T M van Vugt, Steven de Jong
High-grade serous ovarian cancer (HGSOC) has the highest mortality rate among all gynecological cancers. Patients are generally diagnosed in an advanced stage with the majority of cases displaying platinum resistant relapses. Recent genomic interrogation of large numbers of HGSOC patient samples indicated high complexity in terms of genetic aberrations, intra- and intertumor heterogeneity and underscored their lack of targetable oncogenic mutations. Sub-classifications of HGSOC based on expression profiles, termed 'differentiated', 'immunoreactive', 'mesenchymal' and 'proliferative', were shown to have prognostic value...
January 2016: Drug Resistance Updates: Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy
https://www.readbyqxmd.com/read/26702089/erratum-for-the-research-article-tracking-genetically-engineered-lymphocytes-long-term-reveals-the-dynamics-of-t-cell-immunological-memory-by-g-oliveira-e-ruggiero-m-t-l-stanghellini-n-cieri-m-d-agostino-r-fronza-c-lulay-f-dionisio-s-mastaglio-r-greco-j-peccatori
#4
https://www.readbyqxmd.com/read/26659572/tracking-genetically-engineered-lymphocytes-long-term-reveals-the-dynamics-of-t-cell-immunological-memory
#5
Giacomo Oliveira, Eliana Ruggiero, Maria Teresa Lupo Stanghellini, Nicoletta Cieri, Mattia D'Agostino, Mattio D'Agostino, Raffaele Fronza, Christina Lulay, Francesca Dionisio, Sara Mastaglio, Raffaella Greco, Jacopo Peccatori, Alessandro Aiuti, Alessandro Ambrosi, Luca Biasco, Attilio Bondanza, Antonio Lambiase, Catia Traversari, Luca Vago, Christof von Kalle, Manfred Schmidt, Claudio Bordignon, Fabio Ciceri, Chiara Bonini
Long-lasting immune protection from pathogens and cancer requires the generation of memory T cells able to survive long-term. To unravel the immunological requirements for long-term persistence of human memory T cells, we characterized and traced, over several years, T lymphocytes genetically modified to express the thymidine kinase (TK) suicide gene that were infused in 10 patients after haploidentical hematopoietic stem cell transplantation (HSCT). At 2 to 14 years after infusion and in the presence of a broad and resting immune system, we could still detect effectors/effector memory (TEM/EFF), central memory (TCM), and stem memory (TSCM) TK(+) cells, circulating at low but stable levels in all patients...
December 9, 2015: Science Translational Medicine
https://www.readbyqxmd.com/read/26564855/dynamics-of-crispr-cas9-genome-interrogation-in-living-cells
#6
Spencer C Knight, Liangqi Xie, Wulan Deng, Benjamin Guglielmi, Lea B Witkowsky, Lana Bosanac, Elisa T Zhang, Mohamed El Beheiry, Jean-Baptiste Masson, Maxime Dahan, Zhe Liu, Jennifer A Doudna, Robert Tjian
The RNA-guided CRISPR-associated protein Cas9 is used for genome editing, transcriptional modulation, and live-cell imaging. Cas9-guide RNA complexes recognize and cleave double-stranded DNA sequences on the basis of 20-nucleotide RNA-DNA complementarity, but the mechanism of target searching in mammalian cells is unknown. Here, we use single-particle tracking to visualize diffusion and chromatin binding of Cas9 in living cells. We show that three-dimensional diffusion dominates Cas9 searching in vivo, and off-target binding events are, on average, short-lived (<1 second)...
November 13, 2015: Science
https://www.readbyqxmd.com/read/25853023/fluorescence-guided-development-of-a-tricistronic-vector-encoding-bimodal-optical-and-nuclear-genetic-reporters-for-in-vivo-cellular-imaging
#7
Adam Badar, Louise Kiru, Tammy L Kalber, Amit Jathoul, Karin Straathof, Erik Årstad, Mark F Lythgoe, Martin Pule
BACKGROUND: In vivo imaging using genetic reporters is a central supporting tool in the development of cell and gene therapies affording us the ability to selectively track the therapeutic indefinitely. Previous studies have demonstrated the utility of the human norepinephrine transporter (hNET) as a positron emission tomography/single photon emission computed tomography (PET/SPECT) genetic reporter for in vivo cellular imaging. Here, our aim was to extend on this work and construct a tricistronic vector with dual optical (firefly luciferase) and nuclear (hNET) in vivo imaging and ex vivo histochemical capabilities...
2015: EJNMMI Research
https://www.readbyqxmd.com/read/25822024/noninvasive-detection-of-tumor-infiltrating-t-cells-by-pet-reporter-imaging
#8
Melissa N McCracken, Dimitrios N Vatakis, Dhaval Dixit, Jami McLaughlin, Jerome A Zack, Owen N Witte
Adoptive transfer of tumor-reactive T cells can successfully reduce tumor burden; however, in rare cases, lethal on-target/off-tumor effects have been reported. A noninvasive method to track engineered cells with high sensitivity and resolution would allow observation of correct cell homing and/or identification of dangerous off-target locations in preclinical and clinical applications. Human deoxycytidine kinase triple mutant (hdCK3mut) is a nonimmunogenic PET reporter that was previously shown to be an effective tool to monitor whole-body hematopoiesis...
May 2015: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/25798935/multiplexed-tracking-of-combinatorial-genomic-mutations-in-engineered-cell-populations
#9
Ramsey I Zeitoun, Andrew D Garst, George D Degen, Gur Pines, Thomas J Mansell, Tirzah Y Glebes, Nanette R Boyle, Ryan T Gill
Multiplexed genome engineering approaches can be used to generate targeted genetic diversity in cell populations on laboratory timescales, but methods to track mutations and link them to phenotypes have been lacking. We present an approach for tracking combinatorial engineered libraries (TRACE) through the simultaneous mapping of millions of combinatorially engineered genomes at single-cell resolution. Distal genomic sites are assembled into individual DNA constructs that are compatible with next-generation sequencing strategies...
June 2015: Nature Biotechnology
https://www.readbyqxmd.com/read/25653219/in-vivo-tracking-of-t-cells-in-humans-unveils-decade-long-survival-and-activity-of-genetically-modified-t-memory-stem-cells
#10
Luca Biasco, Serena Scala, Luca Basso Ricci, Francesca Dionisio, Cristina Baricordi, Andrea Calabria, Stefania Giannelli, Nicoletta Cieri, Federica Barzaghi, Roberta Pajno, Hamoud Al-Mousa, Alessia Scarselli, Caterina Cancrini, Claudio Bordignon, Maria Grazia Roncarolo, Eugenio Montini, Chiara Bonini, Alessandro Aiuti
A definitive understanding of survival and differentiation potential in humans of T cell subpopulations is of paramount importance for the development of effective T cell therapies. In particular, uncovering the dynamics in vivo in humans of the recently described T memory stem cells (TSCM) would be crucial for therapeutic approaches that aim at taking advantage of a stable cellular vehicle with precursor potential. We exploited data derived from two gene therapy clinical trials for an inherited immunodeficiency, using either retrovirally engineered hematopoietic stem cells or mature lymphocytes to trace individual T cell clones directly in vivo in humans...
February 4, 2015: Science Translational Medicine
https://www.readbyqxmd.com/read/25613483/towards-a-commercial-process-for-the-manufacture-of-genetically-modified-t-cells-for-therapy
#11
REVIEW
A D Kaiser, M Assenmacher, B Schröder, M Meyer, R Orentas, U Bethke, B Dropulic
The recent successes of adoptive T-cell immunotherapy for the treatment of hematologic malignancies have highlighted the need for manufacturing processes that are robust and scalable for product commercialization. Here we review some of the more outstanding issues surrounding commercial scale manufacturing of personalized-adoptive T-cell medicinal products. These include closed system operations, improving process robustness and simplifying work flows, reducing labor intensity by implementing process automation, scalability and cost, as well as appropriate testing and tracking of products, all while maintaining strict adherence to Current Good Manufacturing Practices and regulatory guidelines...
March 2015: Cancer Gene Therapy
https://www.readbyqxmd.com/read/25555152/engineering-and-exploitation-of-a-fluorescent-hiv-1-gp120-for-live-cell-cd4-binding-assays
#12
Lindsey M Costantini, Susan C Irvin, Steven C Kennedy, Feng Guo, Harris Goldstein, Betsy C Herold, Erik L Snapp
The HIV-1 envelope glycoprotein, gp120, binds the host cell receptor, CD4, in the initial step of HIV viral entry and infection. This process is an appealing target for the development of inhibitory drugs and neutralizing antibodies. To study gp120 binding and intracellular trafficking, we engineered a fluorescent fusion of the humanized gp120 JRFL HIV-1 variant and GFP. Gp120-sfGFP is glycosylated with human sugars, robustly expressed, and secreted from cultured human cells. Protein dynamics, quality control, and trafficking can be visualized in live cells...
February 2015: Virology
https://www.readbyqxmd.com/read/25517545/adoptive-immunotherapy-for-hematological-malignancies-using-t-cells-gene-modified-to-express-tumor-antigen-specific-receptors
#13
REVIEW
Hiroshi Fujiwara
Accumulating clinical evidence suggests that adoptive T-cell immunotherapy could be a promising option for control of cancer; evident examples include the graft-vs-leukemia effect mediated by donor lymphocyte infusion (DLI) and therapeutic infusion of ex vivo-expanded tumor-infiltrating lymphocytes (TIL) for melanoma. Currently, along with advances in synthetic immunology, gene-modified T cells retargeted to defined tumor antigens have been introduced as "cellular drugs". As the functional properties of the adoptive immune response mediated by T lymphocytes are decisively regulated by their T-cell receptors (TCRs), transfer of genes encoding target antigen-specific receptors should enable polyclonal T cells to be uniformly redirected toward cancer cells...
December 15, 2014: Pharmaceuticals
https://www.readbyqxmd.com/read/25279468/psma-specific-car-engineered-t-cells-eradicate-disseminated-prostate-cancer-in-preclinical-models
#14
Gaia Zuccolotto, Giulio Fracasso, Anna Merlo, Isabella Monia Montagner, Maria Rondina, Sara Bobisse, Mariangela Figini, Sara Cingarlini, Marco Colombatti, Paola Zanovello, Antonio Rosato
Immunology-based interventions have been proposed as a promising curative chance to effectively attack postoperative minimal residual disease and distant metastatic localizations of prostate tumors. We developed a chimeric antigen receptor (CAR) construct targeting the human prostate-specific membrane antigen (hPSMA), based on a novel and high affinity specific mAb. As a transfer method, we employed last-generation lentiviral vectors (LV) carrying a synthetic bidirectional promoter capable of robust and coordinated expression of the CAR molecule, and a bioluminescent reporter gene to allow the tracking of transgenic T cells after in vivo adoptive transfer...
2014: PloS One
https://www.readbyqxmd.com/read/24817285/optimization-of-in-vitro-cell-labeling-methods-for-human-umbilical-cord-derived-mesenchymal-stem-cells
#15
COMPARATIVE STUDY
R Tao, T-J Sun, Y-Q Han, G Xu, J Liu, Y-F Han
BACKGROUND AND OBJECTIVES: Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are a novel source of seed cells for cell therapy and tissue engineering. However, in vitro labeling methods for hUCMSCs need to be optimized for better detection of transplanted cells. AIM OF THE STUDY: To identify the most stable and efficient method for labeling hUCMSCs in vitro. MATERIALS AND METHODS: hUCMSCs were isolated using a modified enzymatic digestion procedure and cultured...
2014: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/24722773/coxsackievirus-b-exits-the-host-cell-in-shed-microvesicles-displaying-autophagosomal-markers
#16
Scott M Robinson, Ginger Tsueng, Jon Sin, Vrushali Mangale, Shahad Rahawi, Laura L McIntyre, Wesley Williams, Nelson Kha, Casey Cruz, Bryan M Hancock, David P Nguyen, M Richard Sayen, Brett J Hilton, Kelly S Doran, Anca M Segall, Roland Wolkowicz, Christopher T Cornell, J Lindsay Whitton, Roberta A Gottlieb, Ralph Feuer
Coxsackievirus B3 (CVB3), a member of the picornavirus family and enterovirus genus, causes viral myocarditis, aseptic meningitis, and pancreatitis in humans. We genetically engineered a unique molecular marker, "fluorescent timer" protein, within our infectious CVB3 clone and isolated a high-titer recombinant viral stock (Timer-CVB3) following transfection in HeLa cells. "Fluorescent timer" protein undergoes slow conversion of fluorescence from green to red over time, and Timer-CVB3 can be utilized to track virus infection and dissemination in real time...
April 2014: PLoS Pathogens
https://www.readbyqxmd.com/read/24675806/tumor-lysing-genetically-engineered-t-cells-loaded-with-multi-modal-imaging-agents
#17
Parijat Bhatnagar, Mian Alauddin, James A Bankson, Dickson Kirui, Payam Seifi, Helen Huls, Dean A Lee, Aydin Babakhani, Mauro Ferrari, King C Li, Laurence J N Cooper
Genetically-modified T cells expressing chimeric antigen receptors (CAR) exert anti-tumor effect by identifying tumor-associated antigen (TAA), independent of major histocompatibility complex. For maximal efficacy and safety of adoptively transferred cells, imaging their biodistribution is critical. This will determine if cells home to the tumor and assist in moderating cell dose. Here, T cells are modified to express CAR. An efficient, non-toxic process with potential for cGMP compliance is developed for loading high cell number with multi-modal (PET-MRI) contrast agents (Super Paramagnetic Iron Oxide Nanoparticles - Copper-64; SPION-(64)Cu)...
2014: Scientific Reports
https://www.readbyqxmd.com/read/24352938/adoptive-t-cell-therapy-for-hematological-malignancies-using-t-cells-gene-modified-to-express-tumor-antigen-specific-receptors
#18
REVIEW
Hiroshi Fujiwara
The functional properties of the adoptive immune response mediated by effector T lymphocytes are decisively regulated by their T-cell receptors (TCRs). Transfer of genes encoding target antigen-specific receptors enables polyclonal T cells to redirect toward cancer cells and virally infected cells expressing those defined antigens. Using this technology, a large population of redirected T cells displaying uniform therapeutic properties has been produced, powerfully advancing their clinical application as "cellular drugs" for adoptive immunotherapy against cancer...
February 2014: International Journal of Hematology
https://www.readbyqxmd.com/read/24204746/production-and-first-in-man-use-of-t-cells-engineered-to-express-a-hsvtk-cd34-sort-suicide-gene
#19
Hong Zhan, Kimberly Gilmour, Lucas Chan, Farzin Farzaneh, Anne Marie McNicol, Jin-Hua Xu, Stuart Adams, Boris Fehse, Paul Veys, Adrian Thrasher, Hubert Gaspar, Waseem Qasim
UNLABELLED: Suicide gene modified donor T cells can improve immune reconstitution after allogeneic haematopoietic stem cell transplantation (SCT), but can be eliminated in the event of graft versus host disease (GVHD) through the administration of prodrug. Here we report the production and first-in-man use of mismatched donor T cells modified with a gamma-retroviral vector expressing a herpes simplex thymidine kinase (HSVTK):truncated CD34 (tCD34) suicide gene/magnetic selection marker protein...
2013: PloS One
https://www.readbyqxmd.com/read/24023635/engineered-mitochondrial-ferritin-as-a-magnetic-resonance-imaging-reporter-in-mouse-olfactory-epithelium
#20
Bistra Iordanova, T Kevin Hitchens, Clinton S Robison, Eric T Ahrens
We report the design of a MRI reporter gene with applications to non-invasive molecular imaging. We modified mitochondrial ferritin to localize to the cell cytoplasm. We confirmed the efficient cellular processing of this engineered protein and demonstrated high iron loading in mammalian cells. The reporter's intracellular localization appears as distinct clusters that deliver robust MRI contrast. We used this new reporter to image in vivo and ex vivo the gene expression in native olfactory sensory neurons in the mouse epithelium...
2013: PloS One
keyword
keyword
94614
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"