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https://www.readbyqxmd.com/read/29453560/establishment-of-primary-culture-of-sertoli-cells
#1
Raquel L Bernardino, Marco G Alves, Pedro F Oliveira
The successful isolation and culture of Sertoli cells depend on a series of delicate processes of mechanical isolation and enzymatic digestion of the testicular tissue, taking advantage of an array of enzymes (such as DNAse, collagenase, and pancreatin) in order to digest the extracellular matrix components. The complexity of these processes may present some differences depending on the origin of the testicular sample (whole tissue or biopsy) and of the species in question. Rat and mouse Sertoli cells are obtained by a similar protocol, whereas bovine and human Sertoli cells require a more extensive mechanical and enzymatic processing...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29438718/safety-potential-biotechnological-and-probiotic-properties-of-bacteriocinogenic-enterococcus-lactis-strains-isolated-from-raw-shrimps
#2
Olfa Ben Braïek, Stefano Morandi, Paola Cremonesi, Slim Smaoui, Khaled Hani, Taoufik Ghrairi
The aims of this study are to isolate new bacteriocinogenic lactic acid bacterial strains from white (Penaeus vannamei) and pink (Palaemon serratus) raw shrimps and evaluate their technological and probiotic potentialities. Seven strains were selected, among fifty active isolates, as producing interesting antimicrobial activity. Identified as Enterococcus lactis, these isolates were able to produce enterocins A, B and/or P. The safety aspect, assessed by microbiological and molecular tests, demonstrated that the strains were susceptible to relevant antibiotics such as vancomycin, negative for haemolysin and gelatinase activities, and did not harbour virulence and antibiotic resistance genes...
February 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29313579/pancreatic-exocrine-insufficiency-in-type-1-and-2-diabetes-therapeutic-implications
#3
Rupjyoti Talukdar, D Nageshwar Reddy
The objective of the present review is to focus on pancreatic exocrine insufficiency that is associated with Type 1 and 2 diabetes, its clinical and therapeutic implications, including the utility and efficacy of pancreatin supplementation. A literature search was conducted on Pubmed / Medline to identify relevant articles using terms pancreatic exocrine insufficiency in diabetes mellitus patients, pathophysiology, prevalence, treatment and management published between 2006-2016 in English language. Meta-analysis has revealed the prevalence of PEI in patients with type-1 and type-2 diabetes mellitus to be 37...
September 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/29234315/food-associated-lactobacillus-plantarum-and-yeasts-inhibit-the-genotoxic-effect-of-4-nitroquinoline-1-oxide
#4
Roberta Prete, Rosanna Tofalo, Ermanno Federici, Aurora Ciarrocchi, Giovanni Cenci, Aldo Corsetti
Lactic acid bacteria and yeasts, representing the prevailing microbiota associated with different foods generally consumed without any cooking, were identified and characterized in vitro for some functional properties, such as acid-bile tolerance and antigenotoxic activity. In particular, 22 Lactobacillus plantarum strains and 14 yeasts were studied. The gastro-intestinal tract tolerance of all the strains was determined by exposing washed cell suspensions at 37°C to a simulated gastric juice (pH 2.0), containing pepsin (0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29225213/fish-protein-hydrolysate-exhibits-anti-obesity-activity-and-reduces-hypothalamic-neuropeptide-y-and-agouti-related-protein-mrna-expressions-in-rats
#5
Takafumi Mizushige, Masaki Komiya, Moe Onda, Kenji Uchida, Kohsuke Hayamizu, Yukihito Kabuyama
Fish protein is a source of animal protein that is consumed worldwide. Although it has been reported that the intake of Alaska pollack protein (APP) reduces body fat accumulation and increases muscle weight in rats, the mechanisms underlying these effects are poorly understood. As a possibility, peptides released from APP in the gastrointestinal tract are important to the functions of APP. In the present study, we examined the effects of APP hydrolysate digested artificially with pepsin and pancreatin on white adipose tissue and skeletal muscle...
2017: Biomedical Research
https://www.readbyqxmd.com/read/29218505/antibacterial-and-antibiofilm-activity-of-lactic-acid-bacteria-isolated-from-traditional-artisanal-milk-cheese-from-northeast-china-against-enteropathogenic-bacteria
#6
Xingyang Cui, Yunjia Shi, Shanshan Gu, Xin Yan, Hongyan Chen, Junwei Ge
The present study aims to investigate the probiotic properties of novel strains of lactic acid bacteria isolated from traditional artisanal milk cheese from Northeast China and to explore their antibacterial activity against enteropathogenic bacteria. Of the 321 isolates, 86 exhibited survival in low pH, resistance to pancreatin, and tolerance to bile salts; of these, 12 inhibited the growth of more than seven enteropathogenic bacteria and exhibited antibiofilm activities against Staphylococcus aureus CMCC26003 and/or Escherichia coli CVCC230...
December 7, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29183414/effects-of-soluble-fiber-inclusion-in-gestation-diets-with-varying-fermentation-characteristics-on-lactational-feed-intake-of-sows-over-two-successive-parities
#7
C Q Tan, H Q Sun, H K Wei, J J Tan, G Long, S W Jiang, J Peng
The effects of soluble fiber inclusion in gestation diets with varying fermentation characteristics (fermentation kinetics and short-chain fatty acids (SCFA)-profile) on lactational feed intake of sows and their piglet growth over two parities were investigated using an in vitro-in vivo methodology. After breeding, 90 multiparous Landrace sows were randomized to one of three experimental diets: the control (CON) diet, konjac flour (KF) diet or sugar beet pulp (SBP) diet. All diets had similar levels of net energy, CP, insoluble fiber and NDF, but KF and SBP diets had higher soluble fiber levels than the CON diet...
November 29, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29122582/biorelevant-media-slows-the-solution-mediated-phase-transformation-of-amorphous-spironolactone
#8
Mary S Kleppe, Roy J Haskell, Robin H Bogner
Solution-mediated phase transformation (SMPT) can reduce the high drug concentration expected from amorphous formulations, eliminating the improvement in drug absorption one hoped to gain from this high energy drug state. The differences in SMPT of a supersaturating system were compared in biorelevant media (Fasted State Simulated Intestinal Fluid (FaSSIF) and Fed State Simulated Intestinal Fluid (FeSSIF)) and USP compendial medium, Simulated Intestinal Fluid without pancreatin (SIFsp). Amorphous spironolactone underwent SMPT to the same hydrate of spironolactone in all three media which was confirmed by the decrease in dissolution rates assessed in a flow-through dissolution apparatus, as well as by the appearance of crystals on the amorphous solid surface detected by polarized light microscopy...
November 6, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29096438/exploration-of-potentially-bioactive-peptides-generated-from-the-enzymatic-hydrolysis-of-hempseed-proteins
#9
Gilda Aiello, Carmen Lammi, Giovanna Boschin, Chiara Zanoni, Anna Arnoldi
The seed of industrial hemp is an underexploited protein source. In view of a possible use in functional foods, a hempseed protein concentrate was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these enzymes. A detailed peptidomic analysis using data-dependent acquisition showed that the numbers of peptides identified ranged from 90 belonging to 33 parent proteins in the peptic hydrolysate to 9 belonging to 6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme A reductase activity, when tested on the catalytic domain of the enzyme...
November 2, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29090713/pepsin-pancreatin-hydrolysis-reduced-the-ability-of-lunasin-enriched-material-to-inhibit-activation-of-the-inflammasomes-in-thp-1-human-macrophages
#10
Samuel James Price, Philipus Pangloli, Vermont P Dia
Inflammation caused by the NLRP3 inflammasome has been linked to many diseases. Lunasin is a bioactive peptide from soybeans with reported anti-inflammatory properties. The objective of this work was to determine the effect of pepsin-pancreatin hydrolysis (PPH) on the ability of lunasin-enriched preparation (LEP) to inhibit inflammasome activation in differentiated THP-1 human macrophages. THP-1 macrophages were treated with different concentrations of LEP (0.0625 to 0.25 mg mL-1), primed with 1 μg mL-1 lipopolysaccharide for 6 h and activated by 5 mM adenosine triphosphate for 1 h...
November 1, 2017: Food & Function
https://www.readbyqxmd.com/read/29080817/production-and-structural-characterization-of-exopolysaccharides-from-newly-isolated-probiotic-lactic-acid-bacteria
#11
Yousra Abid, Angela Casillo, Houda Gharsallah, Ichrak Joulak, Rosa Lanzetta, Maria Michela Corsaro, Hamadi Attia, Samia Azabou
In this work, four exopolysaccharide-producing lactic acid bacteria (LAB) strains, newly isolated from Tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (BMS and TMS), date palm sap (DPS) and cow milk (CM), were identified as Leuconostoc citreum-BMS, Leuconostoc mesenteroides-TMS, Pediococcus pentosaceus-DPS and Leuconostoc pseudomesenteroides-CM, respectively. The isolated strains showed the ability to withstand simulated human gastrointestinal (GI) tract conditions (low pH, lysozyme, bile salts, pepsin and pancreatin) and showed high surface hydrophobicity (79-90%), besides their ability to act against Escherichia coli and Listeria monocytogenes and to produce exopolysaccharides (EPS)...
October 25, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29068450/soy-peptide-aggregates-formed-during-hydrolysis-reduced-protein-extraction-without-decreasing-their-nutritional-value
#12
Yuanhong Zhang, Feibai Zhou, Mouming Zhao, Zhengxiang Ning, Dongxiao Sun-Waterhouse, Baoguo Sun
Upon enzymatic hydrolysis, soy protein isolates showed a strong tendency to aggregate, presenting a significant loss of valuable proteins. This study mainly focused on the large insoluble aggregates formed during proteolysis, and the influence of heating was further explored for a better understanding of the mechanism involved. The results from SDS-PAGE and amino acid analysis clearly showed that the insoluble aggregates formed upon hydrolysis were aggregated peptides, mainly attributed to the hydrophobic interactions between peptides with hydrophobic amino acids (Val, Ala, Leu, Ile, Tyr, Phe, and Pro) and sulfur-containing (Met and Cys) residues...
December 13, 2017: Food & Function
https://www.readbyqxmd.com/read/29037738/peptides-derived-from-in-vitro-gastrointestinal-digestion-of-germinated-soybean-proteins-inhibit-human-colon-cancer-cells-proliferation-and-inflammation
#13
Marcela González-Montoya, Blanca Hernández-Ledesma, Jose Manuel Silván, Rosalva Mora-Escobedo, Cristina Martínez-Villaluenga
The aim was to investigate the potential of germinated soybean proteins asa source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions>10, 5-10, and<5kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28974795/effect-of-walnut-protein-hydrolysate-on-scopolamine-induced-learning-and-memory-deficits-in-mice
#14
Wenzhi Li, Tiantian Zhao, Jianan Zhang, Jucai Xu, Dongxiao Sun-Waterhouse, Mouming Zhao, Guowan Su
A walnut protein hydrolysate (WPH) was prepared by using a mixture of pancreatin and viscozyme L from industrially available defatted walnut meal. The antioxidant effects of WPH were confirmed and quantified by reducing power, oxygen radical absorbance capacity, hydroxyl radical radical-scavenging activity and ABTS(+)· radical-scavenging activity assays. The protective effects of WPH on scopolamine-induced learning and memory deficits in mice were also evaluated based on in vivo behavioral tests. Results showed that WPH administration would lead to significantly decreased latencies while increased crossing times and target times in the spatial probe test, and increased escape latency and decreased error times in the step-down avoidance test for the scopolamine-induced dementia mice...
September 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28966096/evaluation-of-the-digestibility-of-solid-lipid-nanoparticles-of-glyceryl-dibehenate-produced-by-two-techniques-ultrasonication-and-spray-flash-evaporation
#15
Vincent Jannin, Lucia Blas, Stéphanie Chevrier, Cédric Miolane, Frédéric Demarne, Denis Spitzer
OBJECTIVE: To evaluate the digestibility of Solid Lipid Nanoparticles (SLN) of glyceryl dibehenate prepared either with surfactants by ultrasonication or without surfactant by spray-flash evaporation. METHODS: SLN of glyceryl dibehenate (Compritol® 888 ATO) were produced by two processes: (i) high-shear homogenization with a solution of water-soluble surfactants followed by ultrasonication (ii) and Spray-Flash Evaporation (SFE) of the pure lipid. The digestibility of these nanoparticles was then tested by in vitro lipolysis using a pH-stat apparatus and the assay of glycerides by gel phase chromatography...
September 29, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28946233/impact-of-ultrasonication-on-the-physicochemical-properties-of-sorghum-kafirin-and-in-vitro-pepsin-pancreatin-digestibility-of-sorghum-gluten-like-flour
#16
Andrew C Sullivan, Philipus Pangloli, Vermont P Dia
Sorghum is a nutritionally dense grain but its nutritional quality is limited by its poor digestibility. The objective was to determine the effects of ultrasonication on the physicochemical properties of kafirin, major protein in sorghum, and its stability against pepsin-pancreatin hydrolysis (PPH). Ultrasonication for 10min at 40% amplitude increased the solubility of purified kafirin from 6.5μg/mL to 173.3μg/mL. Ultrasonication altered the secondary structure of kafirin as evaluated by circular dichroism and Fourier-transform infrared red spectroscopy...
February 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28941699/synthesis-of-whey-peptide-iron-complexes-influence-of-using-different-iron-precursor-compounds
#17
Maria Elisa Caetano-Silva, Renata Carolina Alves, Guilherme Nunes Lucena, Regina Célia Galvão Frem, Maria Teresa Bertoldo-Pacheco, Juliana Azevedo Lima-Pallone, Flavia Maria Netto
Iron-binding peptides are an alternative for increasing the bioavailability of iron and to decreasing its pro-oxidant effect. This study aimed to synthesize and characterize peptide-iron complexes using FeCl2 or FeSO4 as the iron precursor compounds. Whey protein isolate (WPI), WPI hydrolyzed with pancreatin, and its fractions obtained via ultrafiltration (cut-off 5kDa) were used as ligands. The fluorescence intensity of the ligands significantly decreased as the iron concentration increased as a result of metal coordination with the iron-binding sites, which may have led to changes in the microenvironment of tryptophan...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28911643/cytotoxic-and-antioxidant-capacity-of-camel-milk-peptides-effects-of-isolated-peptide-on-superoxide-dismutase-and-catalase-gene-expression
#18
Masoud Homayouni-Tabrizi, Ahmad Asoodeh, Mozhgan Soltani
Peptides from natural sources such as milk are shown to have a wide spectrum of biological activities. In this study, three peptides with antioxidant capacity were identified from camel milk protein hydrolysate. Pepsin and pancreatin were used for hydrolysis of milk proteins. Ultrafiltration and reverse-phase high-performance liquid chromatography were used for the concentration and purification of the hydrolysate, respectively. Sequences of the three peptides, which were determined by matrix-assisted laser desorption/ionization time-of-flight spectrophotometry, were LEEQQQTEDEQQDQL [molecular weight (MW): 1860...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28891827/development-of-an-animal-model-for-inducing-various-degrees-of-severity-of-incontinence-associated-dermatitis
#19
Zunjia Wen, Wen Zhu, Qiaoyan Liu, Haiying Zhang, Binbin Mei, Meifen Shen
PURPOSE: The purpose of this study was to describe the biological changes after incontinence-associated dermatitis (IAD) induction by pancreatin in the guinea pigs and to explore the potentially appropriate timing and pancreatin concentration for IAD induction with different severity. DESIGN: In vivo, experimental study. SUBJECTS AND SETTING: An experimental animal model (guinea pig) in a controlled laboratory setting was used for investigation...
November 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/28873632/magnesium-affects-spinach-carotenoid-bioaccessibility-in-vitro-depending-on-intestinal-bile-and-pancreatic-enzyme-concentrations
#20
Joana Corte-Real, Charles Desmarchelier, Patrick Borel, Elke Richling, Lucien Hoffmann, Torsten Bohn
Magnesium may reduce carotenoid bioavailability by forming insoluble complexes with bile salts/fatty acids, inhibiting micelle formation. Here, we investigated whether altering bile/pancreatin concentration influenced potential negative effects of magnesium on carotenoid bioaccessibility. Spinach (4g) was digested in vitro with added magnesium (0, 200, 400mg/L) and canola oil/coffee creamer, at varying bile extract (1 or 8mM) and pancreatin (100 or 990mg/L) concentrations. Bioaccessibility was determined for β-carotene, lutein, and total carotenoids via HPLC...
January 15, 2018: Food Chemistry
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