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https://www.readbyqxmd.com/read/28506800/behaviour-of-silver-nanoparticles-in-simulated-saliva-and-gastrointestinal-fluids
#1
Lucie Pinďáková, Věra Kašpárková, Kristina Kejlová, Markéta Dvořáková, Daniel Krsek, Dagmar Jírová, Lucie Kašparová
Continuously increasing application of silver nanoparticles (AgNPs) requires information on their safety and performance under biological conditions. Assessment of AgNPs in biological systems is also related to availability of robust toxicological methods for evaluation of toxic potential of AgNPs and information on their physicochemical state. Silver nanoparticles were subjected to action of simulated saliva, gastric and intestinal fluids, appropriately supplemented with digestive enzymes pepsin or pancreatin...
May 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28462499/incorporation-of-lipolysis-in-monolayer-permeability-studies-of-lipid-based-oral-drug-delivery-systems
#2
Tanmoy Sadhukha, Buddhadev Layek, Swayam Prabha
Lipid-based drug delivery systems, a well-tolerated class of formulations, have been evaluated extensively to enhance the bioavailability of poorly soluble drugs. However, it has been difficult to predict the in vivo performance of lipid dosage forms based on conventional in vitro techniques such as cell monolayer permeability studies because of the complexity of the gastrointestinal processing of lipid formulations. In the current study, we explored the feasibility of coupling Caco-2 and Madin-Darby canine kidney monolayer permeability studies with lipolysis, a promising in vitro technique to evaluate lipid systems...
May 1, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28460976/in-vitro-versus-in-vivo-protein-digestibility-techniques-for-calculating-pdcaas-protein-digestibility-corrected-amino-acid-score-applied-to-chickpea-fractions
#3
Olga Luisa Tavano, Valdir Augusto Neves, Sinézio Inácio da Silva Júnior
Seven different in vitro methods to determine the protein digestibility for chickpea proteins were considered and also the application of these methodologies for calculating PDCAAS (protein digestibility-corrected amino acid score), seeking their correlations with the in vivo methodology. In vitro digestibility of raw and heated samples were determined using pepsin-pancreatin hydrolysis, considering soluble nitrogen via Kjeldahl (ppKJ) and hydrolysed peptide linkages using trinitrobenzenesulfonic acid and o-phthaldialdehyde...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28407953/antioxidant-potential-of-edible-mushroom-agaricus-bisporus-protein-hydrolysates-and-their-ultrafiltration-fractions
#4
Benard Muinde Kimatu, Liyan Zhao, Yuan Biao, Gaoxing Ma, Wenjian Yang, Fei Pei, Qiuhui Hu
Mushroom protein isolate (MPI) from Agaricus bisporus was hydrolyzed using Alcalase, Pancreatin, Flavourzyme, Alcalase-Pancreatin and Alcalase-Flavourzyme. The obtained hydrolysates (MPHs) were ultrafiltered to generate peptide fractions (UFs) of molecular sizes (<1, 1-3, 3-5 and 5-10kDa). The electrophoretic profile results indicated that the enzymatic systems were efficient in hydrolyzing the MPI into low molecular weight peptides. Hydrolysate yields of >57% and protein recoveries of >43% were obtained...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28375103/antioxidant-and-antiproliferative-activities-of-protein-hydrolysate-from-the-rhizomes-of-zingiberaceae-plants
#5
Kanok Inthuwanarud, Polkit Sangvanich, Songchan Puthong, Aphichart Karnchanatat
Plant proteins have been investigated for their antioxidant activities, but there are still no reports detailing the antioxidant activity levels of plants in the Zingiberaceae family, which are popular food agents and used in folklore medicine. In this study, the crude rhizome protein extract and associated pepsin/pancreatin protein hydrolysate of 15 plants in the Zingiberaceae family were screened using the DPPH method for antioxidant activity. The protein hydrolysate of C. zedoaria possessed the highest antioxidant activity (IC50of 25...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28323499/antiproliferative-activity-of-egg-yolk-peptides-in-human-colon-cancer-cells
#6
Marwa N Yousr, Akram A Aloqbi, Ulfat M Omar, Nazlin K Howell
Egg yolk peptides were successfully prepared from egg yolk protein by-products after lecithin extraction. Defatted egg yolk protein was hydrolyzed with pepsin and pancreatin and purified by gel filtration to produce egg yolk gel filtration fraction (EYGF-33) with antiproliferative activity. The highlight of this study was that the peptide EYGF-33 (1.0 mg/ml) significantly inhibits cell viability of colon cancer cells (Caco-2) with no inhibitory effects on the viability of human colon epithelial normal cells (HCEC) after 48 h...
May 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28259659/bean-peptides-have-higher-in-silico-binding-affinities-than-ezetimibe-for-the-n-terminal-domain-of-cholesterol-receptor-niemann-pick-c1-like-1
#7
Luis M Real Hernandez, Elvira Gonzalez de Mejia
Niemann-Pick C1 like-1 (NPC1L1) mediates cholesterol absorption at the apical membrane of enterocytes through a yet unknown mechanism. Bean, pea, and lentil proteins are naturally hydrolyzed during digestion to produce peptides. The potential for pulse peptides to have high binding affinities for NPC1L1 has not been determined. In this study , in silico binding affinities and interactions were determined between the N-terminal domain of NPC1L1 and 14 pulse peptides (5≥ amino acids) derived through pepsin-pancreatin digestion...
March 1, 2017: Peptides
https://www.readbyqxmd.com/read/28253999/isolation-characterization-and-evaluation-of-the-probiotic-potential-of-a-novel-lactobacillus-strain-isolated-from-feta-type-cheese
#8
Stavros Plessas, Chrysanthi Nouska, Athanasios Karapetsas, Stavros Kazakos, Athanasios Alexopoulos, Ioanna Mantzourani, Pelagia Chondrou, Maria Fournomiti, Alex Galanis, Eugenia Bezirtzoglou
In the present study 45 lactic acid bacteria (LAB) strains were isolated from Feta-type cheese and were screened for probiotic potential in a series of established in vitro tests, including resistance to low pH, resistance to pepsin and pancreatin and tolerance to bile salts. The strain K5, which displayed properties similar to or even better than the reference strain Lactobacillus plantarum ATCC 14917, was chosen for further analysis. Firstly, multiplex PCR analysis indicated that the novel strain belongs to the paracasei species...
July 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28236643/selection-of-indigenous-lactobacillus-paracasei-cd4-and-lactobacillus-gastricus-btm-7-as-probiotic-assessment-of-traits-combined-with-principal-component-analysis
#9
Kavita Sharma, Rishi Mahajan, Sampan Attri, Gunjan Goel
AIMS: The population of Himalayan region is known to consume variety of fermented and non fermented foods and as a result they have been benefited in terms of overall health, because of the associated beneficial microbes. Therefore, the focus of the present study was to identify new strains of Lactic Acid Bacteria (LAB) from dairy products such as milk (cow, goat, buffalo) and fermented products (curd and buttermilk) with properties suitable for use as probiotic cultures. METHODS AND RESULTS: A total of 75 isolates tentatively identified as LAB from 100 samples were initially screened for production of β-hemolysin as indicators of virulence which resulted in 38 isolates with no haemolytic activity...
February 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28219009/inhibition-of-pancreatin-induced-digestion-of-cooked-rice-starch-by-adzuki-vigana-angularis-bean-flavonoids-and-the-possibility-of-a-decrease-in-the-inhibitory-effects-in-the-stomach
#10
Sachiko Hirota, Umeo Takahama
Flavonoids of adzuki bean bind to starch when the beans are cooked with rice. The purpose of this study is to show that adzuki flavonoids can suppress pancreatin-induced digestion of cooked rice starch. The diethyl ether extract of water boiled with adzuki bean inhibited starch digestion, and quercetin and a cyanidin-catechin conjugate (vignacyanidin) but not taxifolin in the extract contributed to the inhibition. The order of their inhibitory effects (taxifolin < quercetin < vignacyanidin) suggested that the effects increased with an increase in their hydrophobicity...
March 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28152036/bioactivity-of-a-modified-human-glucagon-like-peptide-1
#11
Fangfang Xu, Kevin Yueju Wang, Nan Wang, Gangqiang Li, Dehu Liu
Diabetes has become the third largest cause of death in humans worldwide. Therefore, effective treatment for this disease remains a critical issue. Glucagon-like peptide-1 (GLP-1) plays an important role in glucose homeostasis, and therefore represents a promising candidate to use for the treatment of diabetes. Native GLP-1, however, is quickly degraded in in the circulatory system; which limits its clinical application. In the present study, a chemically-synthesized, modified analogue of human GLP-1 (mGLP-1) was designed...
2017: PloS One
https://www.readbyqxmd.com/read/28114854/gastrointestinal-stability-of-dihydromyricetin-myricetin-and-myricitrin-an-in-vitro-investigation
#12
Dong Xiang, Chen-Guang Wang, Wen-Qing Wang, Chun-Yang Shi, Wei Xiong, Meng-Die Wang, Jian-Guo Fang
The gastrointestinal (GI) stability of three flavonoids, dihydromyricetin (DMY), myricetin (MYR), and myricitrin (MYT), was examined in simulated physiological fluids. Several factors that may influence the degradation rate of theses flavonoids were evaluated, including pH and the presence of pepsin and pancreatin enzymes. We found that GI stability followed the order of MYT > DMY > MYR. These flavonoids were stable in simulated gastric fluids and buffer solutions (pH 1.2), but encountered a pseudo-first-order kinetic degradation in simulated intestinal fluids and buffer solutions (pH 6...
January 23, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28049799/pancreatin-trypsin-solution
#13
(no author information available yet)
No abstract text is available yet for this article.
January 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/27988123/short-communication-inhibition-of-angiotensin-1-converting-enzyme-by-peptides-derived-from-variants-of-bovine-%C3%AE-casein-upon-apical-exposure-to-a-caco-2-cell-monolayer
#14
Bjørn Petrat-Melin, Thao T Le, Hanne S Møller, Lotte B Larsen, Jette F Young
This study investigated the consequence of genetically contingent amino acid substitutions in bovine β-casein (CN) genetic variants A(1), A(2), B, and I on the structure and bioactive potential of peptides following in vitro digestion. The β-CN variants were digested in vitro using pepsin and pancreatin, and a peptide profile was obtained by liquid chromatography tandem mass spectrometry, revealing among others, the β-casomorphin precursor peptides VYPFPGPIHN and VYPFPGPIPN, derived from variant A(1)/B and from A(2)/I, respectively...
February 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27977191/hydrolysis-of-dicaffeoylquinic-acids-from-ilex-kudingcha-happens-in-the-colon-by-intestinal-microbiota
#15
Minhao Xie, Guijie Chen, Bing Hu, Li Zhou, Shiyi Ou, Xiaoxiong Zeng, Yi Sun
Monocaffeoylquinic acids (mono-CQAs) can be hydrolyzed or metabolized by pancreatin, intestinal brush border esterase, and microbiota in the colon. Data about the conversion of dicaffeoylquinic acids (diCQAs) in digestion are scarce. The diCQA-rich fraction including 3,4-, 3,5-, and 4,5-diCQAs was prepared from Ilex kudingcha, and the conversion in simulated gastricintestine was investigated. Artificial saliva, gastric and pancreatic fluids, Caco-2 monolayer cells, and anaerobic fermentation model were utilized to mimic digestions of the oral cavity, stomach, small intestine, and colon in vitro...
December 28, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27951472/soy-protein-concentrate-mitigates-markers-of-colonic-inflammation-and-loss-of-gut-barrier-function-in-vitro-and-in-vivo
#16
Zachary T Bitzer, Amy L Wopperer, Benjamin J Chrisfield, Ling Tao, Timothy K Cooper, Jairam Vanamala, Ryan J Elias, John E Hayes, Joshua D Lambert
Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from H2O2-induced cell death and mitigated intracellular oxidative stress. Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases in monolayer permeability...
November 27, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27940083/statistical-investigation-of-simulated-fed-intestinal-media-composition-on-the-equilibrium-solubility-of-oral-drugs
#17
Zhou Zhou, Claire Dunn, Ibrahim Khadra, Clive G Wilson, Gavin W Halbert
Gastrointestinal fluid is a complex milieu and it is recognised that gut drug solubility is different to that observed in simple aqueous buffers. Simulated gastrointestinal media have been developed covering fasted and fed states to facilitate in vitro prediction of gut solubility and product dissolution. However, the combination of bile salts, phospholipids, fatty acids and proteins in an aqueous buffered system creates multiple phases and drug solubility is therefore a complex interaction between these components, which may create unique environments for each API...
March 1, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27933988/niosomes-consisting-of-tween-60-and-cholesterol-improve-the-chemical-stability-and-antioxidant-activity-of-epigallocatechin-gallate-under-intestinal-tract-conditions
#18
Rong Liang, Ling Chen, Wallace Yokoyama, Peter A Williams, Fang Zhong
In order to improve the chemical stability and antioxidant activity of (-)-epigallocatechin gallate (EGCG) in the gastrointestinal tract, niosomes composed of Tween-60 and cholesterol were developed to encapsulate EGCG in this investigation. EGCG loaded niosomes with encapsulation efficiency around 76% exhibited a small Z-average diameter about 60 nm. Compared to free EGCG, the EGCG remaining in dialysis tubes was significantly improved for niosomes at pH 2 and 7.4. Meanwhile, the residual EGCG for niosomes increased from 3% to 49% after 2 h incubation in simulated intestinal fluid (SIF)...
December 7, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27867770/soymilk-residue-okara-as-a-natural-immobilization-carrier-for-lactobacillus-plantarum-cells-enhances-soymilk-fermentation-glucosidic-isoflavone-bioconversion-and-cell-survival-under-simulated-gastric-and-intestinal-conditions
#19
Xia Xiudong, Wang Ying, Liu Xiaoli, Li Ying, Zhou Jianzhong
Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. Okara, a food-grade byproduct from soymilk production, is rich in prebiotics. Lactobacilli could provide health enhancing effects to the host. This study aimed to evaluate the potential of okara as a natural immobilizer for L. plantarum 70810 cells. The study also aimed to evaluate the effects of okara-immobilized L...
2016: PeerJ
https://www.readbyqxmd.com/read/27789354/does-the-commonly-used-ph-stat-method-with-back-titration-really-quantify-the-enzymatic-digestibility-of-lipid-drug-delivery-systems-a-case-study-on-solid-lipid-nanoparticles-sln
#20
Martha Heider, Gerd Hause, Karsten Mäder
Enzymatic digestion of lipid drug carriers is very important. Commonly, pancreatin induced formation of fatty acids is monitored by the pH-stat method, which provides a fast, but unspecific readout. However, according to the literature, the pKa values of long chain fatty acids are strongly dependent on the local environment and might vary between 4.2 and 10.15. The high pKa values would lead to an incomplete detection of the lipid digestion and false results. In order to investigate these issues in more detail, we produced cetyl palmitate solid lipid nanoparticles (CP-SLN) stabilized with poloxamer 188 or polysorbate 80...
December 2016: European Journal of Pharmaceutics and Biopharmaceutics
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