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https://www.readbyqxmd.com/read/28109198/mir-143-induces-the-apoptosis-of-prostate-cancer-lncap-cells-by-suppressing-bcl-2-expression
#1
Zhiwei Ma, Yizhao Luo, Mingxing Qiu
BACKGROUND Prostate cancer has become a serious threat to the life of patients. microRNAs are small non-coding RNA molecules that regulate the growth and apoptosis of cells. We aimed to investigate the regulation and mechanism of microRNA (miR-143) in the proliferation and apoptosis of prostate cancer LNCap cells. MATERIAL AND METHODS miR-143 and control scramble miRNA were synthesized and respectively transfected into LNCap cells. The proliferation and apoptosis were detected by MTT assay, flow cytometry, and caspase-3 activity assay...
January 21, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28109133/the-prognostic-and-therapeutic-application-of-micrornas-in-breast-cancer-tissue-and-circulating-micrornas
#2
REVIEW
Afsane Bahrami, Seyed Amir Aledavoud, Kazem Anvari, Seyed Mahdi Hassanian, Mina Maftouh, Ali Yaghobzade, Omid Salarzaee, Soodabeh ShahidSales, Amir Avan
Breast cancer is the second most common malignancy diagnosed in women, supporting the need for identification of novel prognostic and diagnostic biomarkers. Recently, microRNAs have emerged as molecular regulators that can have key roles in pathogenesis and progression of different malignancies, including breast cancer. Micro-RNAs can be circulated in body fluid, suggesting their values as non-invasive marker. There is growing body of evidence showing the aberrant activation of some known circulating miRNAs, e...
January 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28109115/-research-progress-of-mirnas-targeting-gsk-3%C3%AE-in-regulation-of-hepatocellular-carcinoma-invasion-and-metastasis
#3
Song-Qi He, Bin Wen, Guan-Xin Chen, Hai-Tao Sun, Jia-Ling Sun, Xue-Mei Yang
Invasion and metastasis are key factors contributing to the high mortality rate of patients with hepatocellular carcinoma (HCC) involving a complex mechanism. In the invasion and metastasis of HCC, miRNAs can serve as either oncogenes or tumor suppressor genes to regulate the differentiation and proliferation of tumor cells being and play important roles in tumorigenesis, angiogenesis, invasion and metastasis. This review summarizes the recent progress in research of the molecular mechanisms by which miRNAs targeting GSK-3β regulate HCC invasion and metastasis and examines the roles of miRNAs in hepatocellular carcinoma cell proliferation, apoptosis, invasion, metastasis, and GSK-3β regulation...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109084/mir-5195-3p-inhibits-proliferation-and-invasion-of-human-bladder-cancer-cells-by-directly-targeting-oncogene-klf5
#4
Zhangjie Jiang, Yida Zhang, Runfu Cao, Li Li, Kezhao Zhong, Qingsheng Chen, Jianjun Xiao
MiRNAs played key roles in carcinogenesis of many cancers including bladder cancer. In current study, the role of miR-5195-3p, a quite recently discovered and poorly studied miRNA, in the proliferation and invasion of human bladder cancer cells was investigated. Our data displayed that, compared with the healthy volunteers (control) and SU-HUC-1 human normal bladder epithelial cells, miR-5195-3p was sharply down-regulated in the bladder cancer patients and 5 human bladder cancer cell lines. Then, the oligo miR-5195-3p mimic or miR-5195-3p antagomir was respectively transfected into both T24 and BIU-87 bladder cancer cell lines...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28109082/microrna-133a-inhibits-proliferation-of-gastric-cancer-cells-by-downregulating-erbb2-expression
#5
Chang Li, Xiaoping Li, Shuohui Gao, Chang Li, Lianjun Ma
Gastric cancer is the fourth most common cancer and the second leading cause of cancer-related deaths worldwide. It hasalready been established that miR-133a is involved in gastric cancer. In this study, we aimed to investigate the molecular mechanisms by which miR-133a inhibits the proliferation of gastric cancer cells. We analyzed the proliferative capacity of human gastric cancer cells SNU-1, using MTT assay. Cell apoptosis was determined using flow cytometry. The expression levels of ERBB2, p-ERK1/2 and p-AKT in SNU-1 cells were determined using Western blot analysis...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28109080/microrna-21-inhibits-the-apoptosis-of-osteosarcoma-cell-line-saos-2-via-targeting-caspase-8
#6
Bin Xu, Hehuan Xia, Junming Cao, Zhihong Wang, Yipeng Yang, Yongsheng Lin
Currently, multiple microRNAs (miRNAs) have been found play vital roles in the pathogenesis of osteosarcoma. This studywas aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and correspondingly adjacent tissues were monitored by qPCR. Human osteosarcoma cells line SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival and apoptosis were respectively measured by MTT, colony formation assay, and flow cytometry...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28109076/microrna-409-3p-represses-glioma-cell-invasion-and-proliferation-by-targeting-high-mobility-group-nucleosome-binding-domain-5
#7
Yidong Cao, Liang Zhang, Minghao Wei, Xue Jiang, Dong Jia
Emerging evidence has suggested that aberrantly expressed microRNAs (miRNAs) are associated with glioma development andprogression. The aberrant expression of miR-409-3p has been reported in several human cancers. However, little is known about the function of miR-409-3p in gliomas. The aim of this study was to investigate the specific role and molecular mechanism of miR-409-3p in gliomas. In the present study, we found that miR-409-3p was downregulated in glioma tissue and cell lines. Overexpression of miR-409-3p inhibited glioma cell invasion and proliferation, whereas suppression of miR-409-3p promoted glioma cell invasion and proliferation...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28108674/effects-of-microrna-338-on-morphine-tolerance-by-targeting-cxcr4-in-a-rat-model-of-bone-cancer-pain
#8
Hong-Xia Mei, Min-Hong Zhou, Xing-Wang Zhang, Xi-Xi Huang, Yong-Le Wang, Pei-Fang Wang, Gong-Hao Zhan
This study aimed to investigate the effects of microRNA-338 (miR-338) on morphine tolerance through the targeting of CXCR4 in a rat model of bone cancer pain (BCP). Sprague Dawley rats were obtained and divided into model saline (n = 10), model morphine (n = 50), normal saline (n = 10) and normal morphine (healthy rats, n = 10) groups. After BCP rat model establishment, the remaining SD rats (n = 40) in the model saline group were assigned into pLV-THM-miR-338, pLV-THM-anti-miR-338, CXCR4 shRNA, blank and phosphate buffer saline (PBS) groups...
January 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28108673/plasma-microrna-21-expression-an-indicator-for-the-severity-of-type-2-diabetes-with-diabetic-retinopathy
#9
Qi Jiang, Xue-Man Lv, Yi Yuan, Ling Wang
OBJECTIVE: To investigate the roles of plasma microRNA-21 (miR-21) in the pathogenic process of type 2 diabetes (T2D) with diabetic retinopathy (DR). METHODS: T2D patients included patients without DR (NDR group), patients with non-proliferative/background DR (BDR group) and patients with proliferative DR (PDR group). Healthy individuals served as control group. Fasting plasma glucose (FPG), glycosylated hemoglobin (HbA1c), triglycerides (TG), total cholesterol (TC), urine creatinine (Cr), fasting blood glucose (FBG), blood urea nitrogen (BUN), low-density cholesterol (LDL-C), fasting insulin (FINS) and plasma miR-21 expression were measured...
January 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28108660/from-benchmarking-hits-clip-peak-detection-programs-to-a-new-method-for-identification-of-mirna-binding-sites-from-ago2-clip-data
#10
Silvia Bottini, Nedra Hamouda-Tekaya, Bogdan Tanasa, Laure-Emmanuelle Zaragosi, Valerie Grandjean, Emanuela Repetto, Michele Trabucchi
Experimental evidence indicates that about 60% of miRNA-binding activity does not follow the canonical rule about the seed matching between miRNA and target mRNAs, but rather a non-canonical miRNA targeting activity outside the seed or with a seed-like motifs. Here, we propose a new unbiased method to identify canonical and non-canonical miRNA-binding sites from peaks identified by Ago2 Cross-Linked ImmunoPrecipitation associated to high-throughput sequencing (CLIP-seq). Since the quality of peaks is of pivotal importance for the final output of the proposed method, we provide a comprehensive benchmarking of four peak detection programs, namely CIMS, PIPE-CLIP, Piranha and Pyicoclip, on four publicly available Ago2-HITS-CLIP datasets and one unpublished in-house Ago2-dataset in stem cells...
January 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28108447/mirmine-a-database-of-human-mirna-expression-profiles
#11
Bharat Panwar, Gilbert S Omenn, Yuanfang Guan
MOTIVATION: MicroRNAs (miRNAs) are small non-coding RNAs that are involved in post-transcriptional regulation of gene expression. In this high-throughput sequencing era, a tremendous amount of RNA-seq data is accumulating, and full utilization of publicly available miRNA data is an important challenge. These data are useful to determine expression values for each miRNA, but quantification pipelines are in a primitive stage and still evolving; there are many factors that affect expression values significantly...
January 19, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28108317/silencing-mir-106b-accelerates-osteogenesis-of-mesenchymal-stem-cells-and-rescues-against-glucocorticoid-induced-osteoporosis-by-targeting-bmp2
#12
Ke Liu, Ying Jing, Wen Zhang, Xuejie Fu, Huan Zhao, Xichao Zhou, Yunxia Tao, Huilin Yang, Yan Zhang, Ke Zen, Chenyu Zhang, Donghai Li, Qin Shi
Osteoporosis is a serious health problem worldwide. MicroRNA is a post-transcriptional regulator of gene expression by either promoting mRNA degradation or interfering with mRNA translation of specific target genes. It plays a significant role in the pathogenesis of osteoporosis. Here, we first demonstrated that miR-106b (miR-106b-5p) negatively regulated osteogenic differentiation of mesenchymal stem cells in vitro. Then, we found that miR-106b expression increased in C57BL/6 mice with glucocorticoid-induced osteoporosis (GIOP), and that silencing of miR-106b signaling protected mice against GIOP through promoting bone formation and inhibiting bone resorption...
January 17, 2017: Bone
https://www.readbyqxmd.com/read/28108314/mirna-638-promotes-autophagy-and-malignant-phenotypes-of-cancer-cells-via-directly-suppressing-dact3
#13
Yanli Ren, Yujie Chen, Xue Liang, Yan Lu, Wenting Pan, Ming Yang
Dyregulation of autophagy is implicated in human cancers and the mechanism details remains largely unclear. Herein we report the regulatory role of miR-638 in autophagy of esophageal squamous cell carcinoma (ESCC) and breast cancer cells. We found that miR-638 overexpression promotes starvation- and rapamycin-induced autophagy. In ESCC and breast cancer cells, miR-638 acts as an oncogene and promote cell proliferation, migration, as well as invasion in vitro and in vivo. In accordance with this, we observed significantly higher miR-638 expression in ESCC and breast cancer tissues compared to normal tissues...
January 17, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28108312/ctgf-promotes-osteosarcoma-angiogenesis-by-regulating-mr-543-angiopoietin-2-signaling
#14
Li-Hong Wang, Hsiao-Chi Tsai, Yu-Che Cheng, Chih-Yang Lin, Yuan-Li Huang, Chun-Hao Tsai, Guo-Hong Xu, Shih-Wei Wang, Yi-Chin Fong, Chih-Hsin Tang
Osteosarcoma is the most common primary solid tumor of bone. It has a high metastatic potential and occurs predominantly in adolescents and young adults. Angiopoietin 2 (Angpt2) is a key regulator in tumor angiogenesis, facilitating tumor growth and metastasis. Connective tissue growth factor (CTGF, also known as CCN2), is a cysteine-rich protein that has been reported to promote metastasis of osteosarcoma. However, the effect of CTGF on Angpt2 regulation and angiogenesis in human osteosarcoma remains largely unknown...
January 17, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28108289/negative-feedback-regulation-between-microrna-let-7g-and-lox-1-mediated-hypoxia-induced-pasmcs-proliferation
#15
Wei-Fang Zhang, Tian-Tian Zhu, You-Wen Xiong, Ai-Zhen Xiong, Xiao-Yue Ge, Chang-Ping Hu, Zheng Zhang
BACKGROUND: Pulmonary hypertension (PH) is a proliferative disorder associated with enhanced proliferation and suppressed apoptosis of pulmonary artery smooth muscle cells (PASMCs). Our lately study demonstrated that let-7g inhibited hypoxia-induced proliferation of PASMCs via repressed c-myc-Bmi-1-p16 signaling pathway. However, the upstream of let-7g has not yet been fully defined. Previous studies have shown that LOX-1, a target of let-7g, could also regulate the expression of let-7g in human aortic endothelial cells...
January 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28108181/convergent-repression-of-mir156-by-sugar-and-the-cdk8-module-of-arabidopsis-mediator
#16
Manuel Buendía-Monreal, C Stewart Gillmor
In Arabidopsis, leaves produced during the juvenile vegetative phase are simple, while adult leaves are morphologically complex. The juvenile to adult transition is regulated by miR156, a microRNA that promotes juvenility by impeding the function of SPL transcription factors, which specify adult leaf traits. Both leaf derived sugars, as well as the Mediator Cyclin Dependent Kinase 8 (CDK8) module genes CENTER CITY (CCT)/MED12 and GRAND CENTRAL (GCT)/MED13, act upstream of miR156 to promote the juvenile to adult transition...
January 18, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28107753/role-of-central-opioid-on-the-antinociceptive-effect-of-sulfated-polysaccharide-from-the-red-seaweed-solieria-filiformis-in-induced-temporomandibular-joint-pain
#17
Ianna Wivianne Fernandes Araújo, Hellíada Vasconcelos Chaves, José Mário Pachêco, Danielle Rocha Val, Lorena Vasconcelos Vieira, Rodrigo Santos, Raul Sousa Freitas, Renata Line Rivanor, Valdécio Silvano Monteiro, Juliana Trindade Clemente-Napimoga, Mirna Marques Bezerra, Norma Maria Barros Benevides
This study aimed to investigate the effect of sulfated polysaccharide from red seaweed Solieria filiformis (Fraction F II) in the inflammatory hypernociception in the temporomandibular joint (TMJ) of rats. Male Wistar rats were pretreated (30min) with a subcutaneous injection (s.c.) of vehicle or FII (0.03, 0.3 or 3.0mg/kg) followed by intra-TMJ injection of 1.5% Formalin or 5-hydroxytryptamine (5-HT, 225μg/TMJ). In other set of experiments rats were pretreated (15min) with an intrathecal injection of the non-selective opioid receptors Naloxone, or μ-opioid receptor antagonist CTOP, or δ-opioid receptor Naltridole hydrochloride, or κ-opioid receptor antagonist Nor-Binaltorphimine (Nor-BNI) followed by injection of FII (s...
January 17, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28107707/uremia-modulates-the-phenotype-of-aortic-smooth-muscle-cells
#18
Marie Madsen, Annemarie Aarup, Sebastian Albinsson, Karsten Hartvigsen, Charlotte M Sørensen, Karolina Turczynska, Lars B Nielsen, Tanja Xenia Pedersen
BACKGROUND AND AIMS: Chronic kidney disease leads to uremia and markedly accelerates atherosclerosis. Phenotypic modulation of smooth muscle cells (SMCs) in the arterial media plays a key role in accelerating atherogenesis. The aim of this study was to investigate whether uremia per se modulates the phenotype of aortic SMCs in vivo. METHODS: Moderate uremia was induced by 5/6 nephrectomy in apolipoprotein E knockout (ApoE(-/-)) and wildtype C57Bl/6 mice. Plasma analysis, gene expression, histology, and myography were used to determine uremia-mediated changes in the arterial wall...
December 23, 2016: Atherosclerosis
https://www.readbyqxmd.com/read/28107581/mir-9-plays-a-role-in-il-10-mediated-expression-of-e-cadherin-in-acute-myelogenous-leukemia-cells
#19
Chie Nishioka, Takayuki Ikezoe, Bin Pan, Kailin Xu, Akihito Yokoyama
We previously showed that the CD82/STAT5/IL-10 axis is activated in CD34(+) /CD38(-) acute myelogenous leukemia (AML) cells which favor bone marrow microenvironment. The present study explored the novel biological function of IL-10 in regulation of expression of adhesion molecules in AML cells and found that exposing AML cells to IL-10 induced expression of E-cadherin, but not other adhesion molecules including VLA4, CD29, and LFA1. Downregulation of E-cadherin with a small interfering RNA (siRNA) suppressed the adhesion of leukemia cells to bone marrow-derived mesenchymal stem cells and enhanced the anti-leukemia effect of cytarabine (AraC)...
January 20, 2017: Cancer Science
https://www.readbyqxmd.com/read/28107482/a-circulating-microrna-signature-predicts-age-based-development-of-lymphoma
#20
Afshin Beheshti, Charles Vanderburg, J Tyson McDonald, Charusheila Ramkumar, Tatenda Kadungure, Hong Zhang, Ronald B Gartenhaus, Andrew M Evens
Extensive epidemiological data have demonstrated an exponential rise in the incidence of non-Hodgkin lymphoma (NHL) that is associated with increasing age. The molecular etiology of this remains largely unknown, which impacts the effectiveness of treatment for patients. We proposed that age-dependent circulating microRNA (miRNA) signatures in the host influence diffuse large B cell lymphoma (DLBCL) development. Our objective was to examine tumor development in an age-based DLBCL system using an inventive systems biology approach...
2017: PloS One
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