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https://www.readbyqxmd.com/read/28924030/altered-regulation-of-the-diguanylate-cyclase-yaic-reduces-production-of-type-1-fimbriae-in-a-pst-mutant-of-uropathogenic-e-coli-cft073
#1
Sébastien Crépin, Gaëlle Porcheron, Sébastien Houle, Josée Harel, Charles M Dozois
The pst gene cluster encodes the phosphate specific transport system (Pst). Inactivation of the Pst system constitutively activates the two-component regulatory system PhoBR and attenuates virulence of pathogenic bacteria. In uropathogenic E. coli strain CFT073, attenuation by inactivation of pst is predominantly attributed to the decreased expression of type 1 fimbriae. However, the molecular mechanisms connecting the Pst system and type 1 fimbriae are unknown. To address this, a transposon library was constructed in the pst mutant, and clones were tested for a regain in type 1 fimbriae production...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28922626/the-typhi-colonization-factor-tcf-is-encoded-by-multiple-non-typhoidal-salmonella-serovars-but-exhibits-a-varying-expression-profile-and-interchanging-contribution-to-intestinal-colonization
#2
Shalhevet Azriel, Alina Goren, Inna Shomer, Gili Aviv, Galia Rahav, Ohad Gal-Mor
Salmonella enterica serovars Typhi and Paratyphi A are human-restricted pathogens and the leading causative agents of enteric fever. The Typhi colonization factor (Tcf) is a chaperone-usher fimbria, thought to play a role in the host-specificity of typhoidal serovars. Here we show that the tcf cluster (tcfABCD tinR tioA) is present in at least 25 non-typhoidal Salmonella (NTS) serovars and demonstrate its native expression in clinically-important serovars including Schwarzengrund, 9,12:l,v:-, Choleraesuis, Bredeney, Heidelberg, Montevideo, Virchow and Infantis...
September 18, 2017: Virulence
https://www.readbyqxmd.com/read/28922030/rapid-detection-and-classification-of-salmonella-enterica-shedding-in-feedlot-cattle-utilizing-the-roka-bioscience-atlas-salmonella-detection-assay-for-the-analysis-of-rectoanal-mucosal-swabs
#3
W Evan Chaney, Getahun E Agga, Scott V Nguyen, Terrance M Arthur, Joseph M Bosilevac, Erin Dreyling, Anantharama Rishi, Dayna Brichta-Harhay
With an increasing focus on preharvest food safety, rapid methods are required for the detection and quantification of foodborne pathogens such as Salmonella enterica in beef cattle. We validated the Atlas Salmonella Detection Assay (SEN), a nucleic acid amplification technology that targets Salmonella rRNA, for the qualitative detection of S. enterica with sample enrichment using immunomagnetic separation as a reference test, and we further evaluated its accuracy to predict pathogen load using SEN signal-to-cutoff (SCO) values from unenriched samples to classify animals as high or nonhigh shedders...
September 18, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28919685/prevalence-of-listeria-monocytogenes-yersinia-enterocolitica-staphylococcusaureus-and-salmonella-enterica-typhimurium-in-meat-and-meat-products-using-multiplex-polymerase-chain-reaction
#4
C Latha, C J Anu, V J Ajaykumar, B Sunil
AIM: The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcusaureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR) method. MATERIALS AND METHODS: The assay combined an enrichment step in tryptic soy broth with yeast extract formulated for the simultaneous growth of target pathogens, DNA isolation and multiplex PCR. A total of 1134 samples including beef (n=349), chicken (n=325), pork (n=310), chevon (n=50), and meat products (n=100) were collected from different parts of Kerala, India...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28918634/an-engineered-probiotic-for-the-inhibition-of-salmonella-via-tetrathionate-induced-production-of-microcin-h47
#5
Jacob D Palmer, Emma Piattelli, Beth McCormick, Mark Silby, Christopher Brigham, Vanni Bucci
Complications arising from antibiotic-resistant bacteria are becoming one of the key issues in modern medicine. Members of drug-resistant Enterobacteriaceae spp. include opportunistic pathogens (e.g. Salmonella spp.) that are among the leading causes of morbidity and mortality worldwide. Overgrowth of these bacteria is considered a hallmark of intestinal dysbiosis. Microcins (small antimicrobial peptides) produced by some gut commensals can potentially cure these conditions by inhibiting these pathogens, and have been proposed as a viable alternative to antibiotic treatment...
September 17, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28918274/distribution-comparison-and-risk-assessment-of-free-floating-and-particle-attached-bacterial-pathogens-in-urban-recreational-water-implications-for-water-quality-management
#6
Tingting Fang, Qijia Cui, Yong Huang, Peiyan Dong, Hui Wang, Wen-Tso Liu, Quanhui Ye
The risk of pathogen exposure in recreational water is a concern worldwide. Moreover, suspended particles, as ideal shelters for pathogens, in these waters also need attention. However, the risk caused by the pathogen-particle attachment is largely unknown. Accordingly, water samples in three recreational lakes in Beijing were collected and separated into free-floating (FL, 0.22-5μm) and particle-attached (PA, >5μm) fractions. Next-generation sequencing (NGS) was employed to determine the diversity of genera containing pathogens, and quantitative PCR (qPCR) was used to assess the presence of genes from Escherichia coli (uidA), Salmonella enterica (invA), Aeromonas spp...
September 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28917788/glass-polytetrafluoroethylene-glass-based-sandwich-microdevice-for-continuous-flow-polymerase-chain-reaction-and-its-application-for-fast-identification-of-foodborne-pathogens
#7
Kieu The Loan Trinh, Nae Yoon Lee
A uniquely fabricated microdevice with a glass-polytetrafluoroethylene (PTFE)-glass (GPG) sandwich configuration was developed for the fast identification of foodborne pathogens by using continuous-flow polymerase chain reaction (PCR) followed by on-site fluorescence detection of PCR products. A PTFE tube was simply folded into a serpentine configuration and sandwiched between two glass slides to maintain a 2D planar structure. By using a PTFE tube, the microchannel has the advantage of seamlessness without the need for photolithography methods, and is less expensive and less time-consuming to fabricate than alternative devices...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28915819/bacteriophage-therapy-to-combat-bacterial-infections-in-poultry
#8
REVIEW
Andrzej Wernicki, Anna Nowaczek, Renata Urban-Chmiel
Infections in poultry are an economic and health problem in Europe and worldwide. The most common infections are associated with salmonellosis, colibacillosis, campylobacteriosis, and others. The prevalence of Campylobacter-positive poultry flocks in European countries varies from 18% to 90%. In the United States, the prevalence of infected flocks is nearly 90%. A similar percentage of infection has been noted for salmonellosis (about 75-90%) and E. coli (90-95%). The occurence of Clostridium perfringens is a major problem for the poultry industry, with some estimates suggesting colonization of as many as 95% of chickens, resulting in clinical or subclinical infections...
September 16, 2017: Virology Journal
https://www.readbyqxmd.com/read/28914522/nanozyme-mediated-dual-immunoassay-integrated-with-smartphone-for-use-in-simultaneous-detection-of-pathogens
#9
Nan Cheng, Yang Song, Mohamed Maarouf Ali Zeinhom, Yu-Chung Chang, Lina Sheng, Haolin Li, Dan Du, Lei Li, Meijun Zhu, Yunbo Luo, Wentao Xu, Yuehe Lin
Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, platinum-palladium (Pt-Pd) nanoparticles were applied as signal amplifier in a dual-lateral flow immunoassays (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and E. coli O157:H7. After optimization, the LODs were calculated to be ∼20 CFU/mL for Salmonella Enteritidis and ∼34 CFU/mL for E. coli O157:H7, respectively...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28914098/the-antimicrobial-effects-of-three-phenolic-extracts-from-rosmarinus-officinalis-l-vitis-vinifera-l-and-polygonum-cuspidatum-l-on-food-pathogens
#10
Francesca Santomauro, Cristiana Sacco, Rosa Donato, Maria Bellumori, Marzia Innocenti, Nadia Mulinacci
In this study three phenolic extracts were examined, without volatile fraction, against common food pathogens. The samples, all suitable for food application, were from the leaves of Rosmarinus officinalis L., Vitis vinifera L., and the root of Polygonum cuspidatum L. The microorganisms tested were Escherichia coli O157:H7, Salmonella Enteritidis, Salmonella Typhi, Yersinia enterocolitica and Listeria monocytogenes, well-known as important food pathogens. The results demonstrated a microbicidal activity of all the tested compounds at different concentrations; the rosemary extract showed greater efficacy than the other compounds against the tested microorganisms...
September 15, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28913784/managing-wastes-as-green-resources-cigarette-butt-synthesized-pesticides-are-highly-toxic-to-malaria-vectors-with-little-impact-on-predatory-copepods
#11
Kadarkarai Murugan, Udaiyan Suresh, Chellasamy Panneerselvam, Rajapandian Rajaganesh, Mathath Roni, Al Thabiani Aziz, Jiang-Shiou Hwang, Kuppusamy Sathishkumar, Aruliah Rajasekar, Suresh Kumar, Abdullah A Alarfaj, Akon Higuchi, Giovanni Benelli
The development of novel mosquito control tools is a key prerequisite to build effective and reliable Integrated Vector Management strategies. Here, we proposed a novel method using cigarette butts for the synthesis of Ag nanostructures toxic to young instars of the malaria vector Anopheles stephensi, chloroquine (CQ)-resistant malaria parasites Plasmodium falciparum and microbial pathogens. The non-target impact of these nanomaterials in the aquatic environment was evaluated testing them at sub-lethal doses on the predatory copepod Mesocyclops aspericornis...
September 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28911037/a-biologically-conjugated-polysaccharide-vaccine-delivered-by-attenuated-salmonella-typhimurium-provides-protection-against-challenge-of-avian-pathogenic-escherichia-coli-o1-infection
#12
Yue Han, Qing Liu, Jie Yi, Kang Liang, Yunan Wei, Qingke Kong
Avian pathogenic Escherichia coli (APEC) causes avian airsacculitis and colibacillosis, resulting in significant economic loss to the poultry industry. O1, O2 and O78 are the three predominant serotypes. O-antigen of lipopolysaccharide is serotype determinant and highly immunogenic, and O-antigen polysaccharide-based vaccines have great potential for preventing bacterial infections. In this study, we utilized a novel yeast/bacterial shuttle vector pSS26 to clone the 10.8 kb operon synthesizing APEC O1 O-antigen polysaccharide...
November 30, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28910678/colorimetric-aptasensor-for-the-detection-of-salmonella-enterica-serovar-typhimurium-using-znfe2o4-reduced-graphene-oxide-nanostructures-as-an-effective-peroxidase-mimetics
#13
Shijia Wu, Nuo Duan, Yueting Qiu, Jinghong Li, Zhouping Wang
A new colorimetric aptasensor platform was fabricated to detect Salmonella enterica serovar typhimurium based on the peroxidase-like activity of ZnFe2O4-reduced graphene oxide (ZnFe2O4/rGO) nanostructures. The synthesized ZnFe2O4/rGO can catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 and generate a typical blue product, which was detected by micro-reader at 652nm. Therefore, ZnFe2O4/rGO was conjugated with aptamer to act as the signal probe. Biotin modified aptamer was immobilized onto the micro-plate to act as the capture probe...
September 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28910141/rapid-screening-of-natural-plant-extracts-with-calcium-diacetate-for-differential-effects-against-foodborne-pathogens-and-a-probiotic-bacterium
#14
William Colonna, Byron Brehm-Stecher, Kalidas Shetty, Anthony Pometto Iii
This study focused on advancing a rapid turbidimetric bioassay to screen antimicrobials using specific cocktails of targeted foodborne bacterial pathogens. Specifically, to show the relevance of this rapid screening tool, the antimicrobial potential of generally recognized as safe calcium diacetate (DAX) and blends with cranberry (NC) and oregano (OX) natural extracts was evaluated. Furthermore, the same extracts were evaluated against beneficial lactic acid bacteria. The targeted foodborne pathogens evaluated were Escherichia coli O157:H7, Salmonella spp...
September 14, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28910140/impact-of-clinical-salmonellosis-in-veal-calves-on-the-recovery-of-salmonella-in-lymph-nodes-at-harvest
#15
Lohendy Muñoz-Vargas, Sarah K Finney, Holden Hutchinson, Margaret A Masterson, Greg Habing
The objective of this study was to determine the prevalence, serotypes, antimicrobial resistance phenotypes, and pulsed-field gel electrophoresis (PFGE) patterns of Salmonella recovered in feces and mesenteric and prefemoral lymph nodes (LNs) from cohorts of calves with and without a confirmed outbreak of salmonellosis. In a prospective cohort study, 160 calves from four farms without a reported outbreak (nonoutbreak farms) were sampled at farm and harvest. In addition, harvest samples from 80 calves of two farms with a confirmed outbreak (outbreak farms) were collected...
September 14, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28904956/application-of-the-subtractive-genomics-and-molecular-docking-analysis-for-the-identification-of-novel-putative-drug-targets-against-salmonella-enterica-subsp-enterica-serovar-poona
#16
Tanvir Hossain, Mohammad Kamruzzaman, Talita Zahin Choudhury, Hamida Nooreen Mahmood, A H M Nurun Nabi, Md Ismail Hosen
The emergence of novel pathogenic strains with increased antibacterial resistance patterns poses a significant threat to the management of infectious diseases. In this study, we aimed at utilizing the subtractive genomic approach to identify novel drug targets against Salmonella enterica subsp. enterica serovar Poona strain ATCC BAA-1673. We employed in silico bioinformatics tools to subtract the strain-specific paralogous and host-specific homologous sequences from the bacterial proteome. The sorted proteome was further refined to identify the essential genes in the pathogenic bacterium using the database of essential genes (DEG)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28901372/multivalent-gold-nanoparticle-peptide-conjugates-for-targeting-intracellular-bacterial-infections
#17
Rimi Chowdhury, Humaira Ilyas, Anirban Ghosh, Haydar Ali, Arup Ghorai, Anupam Midya, Nikhil R Jana, Santasabuj Das, Anirban Bhunia
Although nanoparticle-tagged antimicrobal peptides have gained considerable importance in recent years, their structure-function correlation has not yet been explored. Here, we have studied the mechanism of action of a designed antimicrobial peptide, VG16KRKP (VARGWKRKCPLFGKGG), delivered via gold nanoparticle tagging against Salmonella infection by combining biological experiments with high- and low-resolution spectroscopic techniques. In comparison with the free VG16KRKP peptide or gold nanoparticle alone, the conjugated variant, Au-VG16KRKP, is non-cytotoxic to eukaryotic cells, but exhibits strong bacteriolytic activity in culture...
September 13, 2017: Nanoscale
https://www.readbyqxmd.com/read/28900347/campylobacter-from-microbiology-to-prevention
#18
REVIEW
A Facciolà, R Riso, E Avventuroso, G Visalli, S A Delia, P Laganà
In last years, Campylobacter spp has become one of the most important foodborne pathogens even in high-income countries. Particularly, in Europe, Campylobacteriosis is, since 2005, the foodborne disease most frequently notified and the second in USA, preceded by the infection due to Salmonella spp. Campylobacter spp is a commensal microorganism of the gastrointestinal tract of many wild animals (birds such as ducks and gulls), farm animals (cattle and pigs) and companion animals (such as dogs and cats) and it is responsible for zoonoses...
June 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28898289/trim32-tax1bp1-dependent-selective-autophagic-degradation-of-trif-negatively-regulates-tlr3-4-mediated-innate-immune-responses
#19
Qing Yang, Tian-Tian Liu, Heng Lin, Man Zhang, Jin Wei, Wei-Wei Luo, Yun-Hong Hu, Bo Zhong, Ming-Ming Hu, Hong-Bing Shu
Toll-like receptor (TLR)-mediated signaling are critical for host defense against pathogen invasion. However, excessive responses would cause harmful damages to the host. Here we show that deficiency of the E3 ubiquitin ligase TRIM32 increases poly(I:C)- and LPS-induced transcription of downstream genes such as type I interferons (IFNs) and proinflammatory cytokines in both primary mouse immune cells and in mice. Trim32-/- mice produced higher levels of serum inflammatory cytokines and were more sensitive to loss of body weight and inflammatory death upon Salmonella typhimurium infection...
September 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28895672/meat-and-meat-products-%C3%A2-analysis-of-the-most-common-threats-in-the-years-2011-2015-in-rapid-alert-system-for-food-and-feed-rasff
#20
Anna D. Kononiuk, Małgorzata Karwowska
Background: The key tool used in the European Union in order to eliminate the risks associated with the consumption of potentially hazardous food is RASFF - Rapid Alert System for Food and Feed Safety. The RASFF was established to increase accountability and strengthening cooperation between states of the European Union in the field of food safety control. Objective: The aim of this study was to explore the trends and temporal and spatial distribution of notifications on food safety hazards between January 2011 and December 2015 with a special emphasis on meat and meat products on the basis of notification from RASFF...
2017: Roczniki Państwowego Zakładu Higieny
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