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https://www.readbyqxmd.com/read/28330283/multiplex-pcr-for-simultaneous-identification-of-e-coli-o157-h7-salmonella-spp-and-l-monocytogenes-in-food
#1
Thuy Trang Nguyen, Vo Van Giau, Tuong Kha Vo
The rapid detection of pathogens in food is becoming increasingly critical for ensuring the safety of consumers, since the majority of food-borne illnesses and deaths are caused by pathogenic bacteria. Hence, rapid, sensitive, inexpensive and convenient approaches to detect food-borne pathogenic bacteria is essential in controlling food safety. In this study, a multiplex PCR assay for the rapid and simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes was established...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330281/evaluation-of-bioactive-secondary-metabolites-from-endophytic-fungus-pestalotiopsis-neglecta-bab-5510-isolated-from-leaves-of-cupressus-torulosa-d-don
#2
Deeksha Sharma, Avijit Pramanik, Pavan Kumar Agrawal
Six endophytic fungi were isolated from Cupressus torulosa D.Don and identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the agar well diffusion method against human pathogen such as Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus aureus. Isolated strain of Pestalotiopsis sp. was showing prominent antibacterial activity. The crude methanol and ethyl acetate extract of Pestalotiopsis sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330077/diversity-and-antagonistic-potential-of-marine-microbes-collected-from-south-west-coast-of-india
#3
S Sinimol, A R Sarika, A Jayakumaran Nair
The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329249/acetylation-regulates-protein-stability-and-dna-binding-ability-of-hild-to-modulate-salmonella-typhimurium-virulence
#4
Yu Sang, Jie Ren, Ran Qin, Shuting Liu, Zhongli Cui, Sen Cheng, Xiaoyun Liu, Jie Lu, Jing Tao, Yu-Feng Yao
HilD, a dominant regulator of Salmonella pathogenicity island 1 (SPI-1), can be acetylated by acetyltransferase Pat in Salmonella Typhimurium, and the acetylation is beneficial to its stability. However, the underlying mechanism of HilD stability regulated by acetylation is not clear. We show here that lysine 297 (K297) located in the helix-turn-helix motif, can be acetylated by Pat. Acetylation of K297 increases HilD stability, but reduces its DNA-binding affinity. In turn, the deacetylated K297 enhances the DNA-binding ability, but decreases HilD stability...
February 24, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28323855/etiology-of-acute-diarrhea-in-the-elderly-in-china-a-six-year-observational-study
#5
Zike Zhang, Shengjie Lai, Jianxing Yu, Qibin Geng, Wanqi Yang, Yu Chen, Jianguo Wu, Huaiqi Jing, Weizhong Yang, Zhongjie Li
Acute diarrhea leads to a substantial disease burden among the elderly worldwide. However, in the context of increasingly aging trend in China, the prevalence of etiological agents among elderly diarrheal patients was undetermined. This study aimed to explore the major enteropathogens of acute diarrhea among outpatients older than 65 years in China, and also the epidemiological features of the pathogens. Demographic and clinical data for acute diarrhea among outpatients older than 65 years were collected from 213 participating hospitals from 2009 to 2014...
2017: PloS One
https://www.readbyqxmd.com/read/28322256/ag-ago-nanoparticles-grown-via-time-dependent-double-mechanism-in-a-2d-layered-ni-pcp-and-their-antibacterial-efficacy
#6
Rashmi A Agarwal, Neeraj K Gupta, Rajan Singh, Shivansh Nigam, Bushra Ateeq
A simple synthesis route for growth of Ag/AgO nanoparticles (NPs) in large quantitative yields with narrow size distribution from a functional, non-activated, Ni (II) based highly flexible porous coordination polymer (PCP) as a template has been demonstrated. This template is a stable storage media for the NPs larger than the pore diameters of the PCP. From EPR study it was concluded that NPs were synthesized via two mechanisms i.e. acid formation and the redox activity of the framework. Size range of Ag/AgO NPs is sensitive to choice of solvent and reaction time...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28319869/biofilm-inhibition-formation-of-clinical-strains-of-pseudomonas-aeruginosa-mutans-photocatalytic-activity-of-azo-dye-and-gc-ms-analysis-of-leaves-of-lagerstroemia-speciosa
#7
V Sai Saraswathi, Neethu Kamarudheen, K V Bhaskara Rao, K Santhakumar
The investigation was conducted to analyse the bioactive compounds from the leaf extracts of L. speciosa by GC-MS. The extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. The bioactive compounds from the leaves of L. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by Gas Chromatography-Mass Spectroscopy (GC-MS). The antibacterial activity was evaluated against clinical strain like Staphylococcus aureus, Escherichia coli, P...
March 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28319799/occurrence-of-hepatitis-a-and-e-and-norovirus-gi-and-gii-in-ready-to-eat-vegetables-in-italy
#8
V Terio, M Bottaro, E Pavoni, M N Losio, A Serraino, F Giacometti, V Martella, A Mottola, A Di Pinto, G Tantillo
Fresh vegetables and their ready-to-eat (RTE) salads have become increasingly recognized as potential vehicles for foodborne diseases. The EU Reg. 1441/2007 establishes microbiological criteria for bacterial pathogens for products placed on the market during their shelf-life (i.e. Salmonella spp., Listeria monocytogenes) for pre-cut fruits and vegetables (RTE) whilst it does not address the problem of contamination by enteric viruses. In this study we investigated the contamination by hepatitis A virus (HAV), hepatitis E virus (HEV) and norovirus (NoV) in 911 ready-to-eat vegetable samples taken from products at retail in Apulia and in Lombardia...
March 14, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28318301/comparative-study-on-removal-of-enteric-pathogens-from-domestic-wastewater-using-typha-latifolia-and-cyperus-rotundus-along-with-different-substrate
#9
Rita P Shingare, Sneha V Nanekar, Prashant R Thawale, R Karthik, Asha A Juwarkar
A comparative study was carried out over a period of six months to evaluate the efficiency of different substrate material along with macrophytes Typha latifolia and Cyperus rotundus in treating domestic wastewater intended for reuse in agriculture. The study was conducted over a period of six months with different retention time and observations were taken twice per month. One-way ANOVA and Tukey's HSD tests were used to determine statistical significant differences between experimental columns. Treatment with T...
March 20, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28317440/potent-immune-responses-induced-by-a-salmonella-ghost-delivery-system-that-expresses-the-recombinant-stx2eb-fedf-and-feda-proteins-of-the-escherichia-coli-producing-f18-and-shiga-toxin-in-a-murine-model-and-evaluation-of-its-protective-effect-as-a-porcine-vaccine
#10
Gayeon Won, John Hwa Lee
BACKGROUND: In the pathogenicity of porcine edema disease, which is caused by the Escherichia coli-producing F18 and Shiga toxin, F18(+) fimbrial adhesins and Shiga toxin 2e (Stx2e) play pivotal roles in the colonization and enterotoxicity of this pathogen. OBJECTIVE: To develop a vaccine candidate against edema disease by combining three selected antigens of F18(+) Shiga toxin-producing Escherichia coli (STEC). METHODS: Genetically engineered Salmonella Typhimurium (ST) ghosts that express Stx2eB, FedF, and FedA were individually inserted in a ghost plasmid cassette (pJHL184), and the resultant plasmids were transformed into a Δasd Δlon ΔcpxR ST (JOL912)...
March 20, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28302859/inflammation-boosts-bacteriophage-transfer-between-salmonella-spp
#11
Médéric Diard, Erik Bakkeren, Jeffrey K Cornuault, Kathrin Moor, Annika Hausmann, Mikael E Sellin, Claude Loverdo, Abram Aertsen, Martin Ackermann, Marianne De Paepe, Emma Slack, Wolf-Dietrich Hardt
Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. A murine Salmonella Typhimurium (STm) diarrhea model was used to study the transfer of SopEΦ, a prophage from STm SL1344, to STm ATCC14028S. Gut inflammation and enteric disease triggered >55% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEΦ transfer was reduced by up to 10(5)-fold...
March 17, 2017: Science
https://www.readbyqxmd.com/read/28302471/the-expanding-targetome-of-small-rnas-in-salmonella-typhimurium
#12
REVIEW
Daniel Ryan, Mohana Mukherjee, Mrutyunjay Suar
The enterobacterial pathogen Salmonella has long served as a model for bacterial pathogenesis, stress response, gene expression and regulation with extensive investigation involving protein function. With the advent of high-throughput sequencing technologies and their applications in genomics and transcriptomics, a wealth of data particularly with respect to small RNAs (sRNAs) is being generated. These molecules serve as regulators of major stress response and virulence networks in diverse species including Salmonella enterica subsp...
March 13, 2017: Biochimie
https://www.readbyqxmd.com/read/28302440/kidney-allograft-pyelonephritis-caused-by-salmonella-enterica-serovar-schwarzengrund
#13
Kenta Ito, Haruomi Nishio, Yuji Iwatani, Ryo Yamada, Takao Okawa, Takumi Yamamoto, Masaaki Murakami, Yoko Matsuo, Ken Matsuo, Satoshi Tanaka, Kiyoshi Mori, Noriko Mori
Kidney transplant recipients (KTRs) taking immunosuppressive drugs have a 20-fold greater risk of nontyphoidal Salmonella (NTS) infection than the healthy adult population. Among KTRs, the urinary tract is the most common site of infection. However, few cases of urinary tract infection caused by NTS have been documented in KTRs, and only one in Japan. Furthermore, it frequently induces acute allograft rejection with high mortality. Salmonella enterica subsp. enterica serovar Schwarzengrund (S. Schwarzengrund) is now among the more common Salmonella serovars isolated in Japan and is likely to be invasive...
March 13, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28301453/investigation-of-salmonella-enteritidis-outbreak-associated-with-truffle-oil-district-of-columbia-2015
#14
S Janet Kuramoto-Crawford, Sasha McGee, Keith Li, Andrew K Hennenfent, Kossia Dassie, Jhetari T Carney, Arian Gibson, Ivory Cooper, Morris Blaylock, Reginald Blackwell, Angela Fields, John Davies-Cole
On September 8, 2015, the District of Columbia Department of Health (DCDOH) received a call from a person who reported experiencing gastrointestinal illness after eating at a District of Columbia (DC) restaurant with multiple locations throughout the United States (restaurant A). Later the same day, a local emergency department notified DCDOH to report four persons with gastrointestinal illness, all of whom had eaten at restaurant A during August 30-September 5. Two patients had laboratory-confirmed Salmonella group D by stool culture...
March 17, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28300643/draft-genome-sequence-of-multidrug-resistant-salmonella-enterica-serovar-brancaster-strain-ps01-isolated-from-chicken-meat-malaysia
#15
Pui San Chin, Choo Yee Yu, Geik Yong Ang, Wai-Fong Yin, Kok-Gan Chan
OBJECTIVES: Salmonella spp. represent one of the main diarrhoeal pathogens that are transmitted via the food supply chain. Here we report the draft genome sequence of a multidrug-resistant Salmonella enterica serovar Brancaster (PS01) that was isolated from poultry meat in Malaysia. METHODS: Genomic DNA was extracted from Salmonella strain PS01 and was sequenced using an Illumina HiSeq 2000 platform. The generated reads were de novo assembled using CLC Genomics Workbench...
March 11, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28298258/susceptibility-to-rifaximin-and-other-antimicrobials-of-bacteria-isolated-in-patients-with-acute-gastrointestinal-infections-in-southeast-mexico
#16
O Novoa-Farias, A C Frati-Munari, M A Peredo, S Flores-Juárez, O Novoa-García, J Galicia-Tapia, C E Romero-Carpio
INTRODUCTION: Enteropathogenic bacteria isolated in Mexico City have shown a high rate of resistance to different antibiotics, with the exception of rifaximin (RIF). RIF is a nonabsorbable antibiotic that reaches high fecal concentrations (≈ 8,000μg/g). Susceptibility to antimicrobials can vary in different geographic regions. AIM: To study the susceptibility to rifaximin and other antimicrobials of enteropathogenic bacteria isolated in patients with acute diarrhea in the southeastern region of Mexico...
March 12, 2017: Revista de Gastroenterología de México
https://www.readbyqxmd.com/read/28298180/bacterial-protein-meta-interactomes-predict-cross-species-interactions-and-protein-function
#17
J Harry Caufield, Christopher Wimble, Semarjit Shary, Stefan Wuchty, Peter Uetz
BACKGROUND: Protein-protein interactions (PPIs) can offer compelling evidence for protein function, especially when viewed in the context of proteome-wide interactomes. Bacteria have been popular subjects of interactome studies: more than six different bacterial species have been the subjects of comprehensive interactome studies while several more have had substantial segments of their proteomes screened for interactions. The protein interactomes of several bacterial species have been completed, including several from prominent human pathogens...
March 16, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28296382/human-%C3%AE-defensin-6-a-small-peptide-that-self-assembles-and-protects-the-host-by-entangling-microbes
#18
Phoom Chairatana, Elizabeth M Nolan
Human α-defensin 6 (HD6) is a 32-residue cysteine-rich peptide that contributes to innate immunity by protecting the host at mucosal sites. This peptide is produced in small intestinal Paneth cells, stored as an 81-residue precursor peptide named proHD6 in granules, and released into the lumen. One unusual feature of HD6 is that it lacks the broad-spectrum antimicrobial activity observed for other human α-defensins, including the Paneth cell peptide human α-defensin 5 (HD5). HD6 exhibits unprecedented self-assembly properties, which confer an unusual host-defense function...
March 15, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28296192/inferring-source-attribution-from-a-multiyear-multisource-data-set-of-salmonella-in-minnesota
#19
C Ahlstrom, P Muellner, S E F Spencer, S Hong, A Saupe, A Rovira, C Hedberg, A Perez, U Muellner, J Alvarez
Salmonella enterica is a global health concern because of its widespread association with foodborne illness. Bayesian models have been developed to attribute the burden of human salmonellosis to specific sources with the ultimate objective of prioritizing intervention strategies. Important considerations of source attribution models include the evaluation of the quality of input data, assessment of whether attribution results logically reflect the data trends and identification of patterns within the data that might explain the detailed contribution of different sources to the disease burden...
March 13, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28295924/flagellin-phase-dependent-swimming-on-epithelial-cell-surfaces-contributes-to-productive-salmonella-gut-colonization
#20
Julia A Horstmann, Erik Zschieschang, Theresa Truschel, Juana de Diego, Michele Lunelli, Manfred Rohde, Tobias May, Till Strowig, Theresia Stradal, Michael Kolbe, Marc Erhardt
The flagellum is a sophisticated nanomachine and an important virulence factor of many pathogenic bacteria. Flagellar motility enables directed movements towards host cells in a chemotactic process and near surface swimming on cell surfaces is crucial for selection of permissive entry sites. The long external flagellar filament is made of tens of thousands subunits of a single protein, flagellin, and many Salmonella serovars alternate expression of antigenically distinct flagellin proteins, FliC and FljB. However, the role of the different flagellin variants during gut colonization and host cell invasion remains elusive...
March 13, 2017: Cellular Microbiology
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