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NEC blood biomarkers

Birte Tröger, Christoph Härtel, Jan Buer, Michael Dördelmann, Ursula Felderhoff-Müser, Thomas Höhn, Nico Hepping, Georg Hillebrand, Angela Kribs, Janina Marissen, Dirk Olbertz, Peter-Michael Rath, Susanne Schmidtke, Jens Siegel, Egbert Herting, Wolfgang Göpel, Joerg Steinmann, Anja Stein
INTRODUCTION: In the German Neonatal Network (GNN) 10% of very-low-birth weight infants (VLBWI) suffer from blood-culture confirmed sepsis, while 30% of VLBWI develop clinical sepsis. Diagnosis of sepsis is a difficult task leading to potential over-treatment with antibiotics. This study aims to investigate whether the results of blood multiplex-PCR (SeptiFast®) for common sepsis pathogens are relevant for clinical decision making when sepsis is suspected in VLBWI. METHODS: We performed a prospective, multi-centre study within the GNN including 133 VLBWI with 214 episodes of suspected late onset sepsis (LOS)...
2016: PloS One
José Luis Gómez-Chaparro Moreno, Alejandro Rodríguez Torronteras, María Dolores Ruiz González, Lucía Izquierdo Palomares, Daniel Bonilla Valverde, Julia Ruiz Laguna, Alfonso Delgado Rubio, Juan López-Barea
UNLABELLED: We aimed to establish the utility of serum cytosolic β-glycosidase (CBG) assay as a NEC diagnosis tool. CBG activity has been compared in 192 NEC-free (NEC(-)) and 13 NEC-affected (NEC(+)) neonates, with modified Bell's stages II/III, born at Reina Sofia University Hospital; additional blood hematology, microbiology, and biochemical parameters have been assayed. NEC(+) neonates have higher serum CBG activity, 26.4 ± 12.4 mU/mg; 95 % CI (18.8-33.9), than NEC(-) infants, 11...
July 2016: European Journal of Pediatrics
Stefano Giuliani, Yew-Wei Tan, Dongling Zheng, Evmorfia Petropoulou, Ali Sohail, Sarah Bradley, Justin Richards, Nigel Kennea, Yalda Jamshidi
OBJECTIVES: Coagulopathy and mesenteric thrombosis are common in premature neonates with necrotizing enterocolitis (NEC). This pilot study aimed to investigate the hypothesis that there are changes in the gene expression related to the coagulation and anticoagulation systems in NEC. METHODS: Consecutive neonates (n = 11) with NEC (Bell's stages 2-3) were recruited. Two comparison groups, matched for birth weight and corrected gestational age, were selected based on: the absence of inflammation and coagulopathy (healthy control, n = 10); or the presence of a confirmed blood infection (sepsis control, n = 12)...
March 31, 2016: Journal of Pediatric Gastroenterology and Nutrition
Christina N Cordeiro, Yulia Savva, Dhananjay Vaidya, Cynthia H Argani, Xiumei Hong, Xiaobin Wang, Irina Burd
PROBLEM: To identify preterm neonates at risk for adverse neonatal outcomes. METHOD OF STUDY: A nested case-control study from the prospectively followed Boston Birth Cohort of mother-neonate pairs was performed. A classification model for preterm-born neonates was derived from 27 cord blood biomarkers using orthogonal projections to latent structures discriminant analysis. Predictive relationships were made between biomarkers and adverse outcomes using logistic regression...
May 2016: American Journal of Reproductive Immunology: AJRI
Guo-Zhong Tao, Bo Liu, Rong Zhang, Gigi Liu, Fizan Abdullah, Mary Cay Harris, Mary L Brandt, Richard A Ehrenkranz, Corinna Bowers, Camilia R Martin, R Lawrence Moss, Karl G Sylvester
Necrotizing enterocolitis (NEC) is the most common gastrointestinal (GI) medical/surgical emergency of the newborn and a leading cause of preterm neonate morbidity and mortality. NEC is a challenge to diagnose since it often shares similar clinical features with neonatal sepsis. In the present study, plasma protein profiling was compared among NEC, sepsis and control cohorts using gel electrophoresis, immunoblot and mass spectrometry. We observed significant impairment in the formation of fibrinogen-γ dimers (FGG-dimer) in the plasma of newborns with NEC that could efficiently differentiate NEC and sepsis with a high level of sensitivity and specificity...
2015: Scientific Reports
Lila S Wahidi, Jan Sherman, Mindy M Miller, Habib Zaghouani, Michael P Sherman
BACKGROUND: Eosinophils infiltrate intestinal tissue during necrotizing enterocolitis (NEC) and adult bowel diseases. We theorized that epithelial damage causes eosinophilic activation and recruitment at NEC onset. OBJECTIVE: We studied the relationship between persistent blood eosinophilia and medical or surgical complications during NEC. METHODS: NEC cases and controls at MU Children's Hospital (2008-2013) underwent review. A Likert scale measured NEC severity...
2015: Neonatology
Serafina Perrone, Maria Luisa Tataranno, Antonino Santacroce, Simona Negro, Giuseppe Buonocore
Necrotizing enterocolitis (NEC) is a devastating and common disease of very low birth weight (VLBW) infants with a mortality rate of 10% to 50% and a significant cause of morbidity in survivors. The incidence of NEC has increased from 5% to 7% in the last decades and this rate is likely to rise because of the increased survival of infants born at 24 weeks gestation, which are at high risk of developing NEC. NEC etiology is multifactorial: ischemia, infections, cytokines, enteral feeding and reactive oxygen species or free radicals (FRs) may contribute to the disruption of the immature gut barrier...
2014: Current Pediatric Reviews
J Remon, R Kampanatkosol, R R Kaul, J K Muraskas, R D Christensen, A Maheshwari
OBJECTIVE: Necrotizing enterocolitis (NEC) is characterized by macrophage infiltration into affected tissues. Because intestinal macrophages are derived from recruitment and in situ differentiation of blood monocytes in the gut mucosa, we hypothesized that increased recruitment of monocytes to the intestine during NEC reduces the blood monocyte concentration and that this fall in blood monocytes can be a useful biomarker for NEC. STUDY DESIGN: We reviewed medical records of very-low-birth-weight (VLBW) infants treated for NEC and compared them with a matched control group comprised of infants with feeding intolerance but no signs of NEC...
July 2014: Journal of Perinatology: Official Journal of the California Perinatal Association
Kostan W Reisinger, Boris W Kramer, David C Van der Zee, Hens A A Brouwers, Wim A Buurman, Ernest van Heurn, Joep P M Derikx
OBJECTIVE: To evaluate the value of biomarkers to detect severe NEC. SUMMARY BACKGROUND DATA: The time point of surgery in necrotizing enterocolitis (NEC) is critical. Therefore, there is a need for markers that detect severe NEC, because clinical signs of severe NEC often develop late. This study evaluated the value of biomarkers reflecting intestinal cell damage and inflammation to detect severe NEC. METHODS: 29 neonates with NEC were included...
2014: PloS One
Richard Kampanatkosol, Tricia Thomson, Omar Habeeb, Loretto Glynn, Phillip J Dechristopher, Sherri Yong, Walter Jeske, Akhil Maheshwari, Jonathan Muraskas
BACKGROUND: Necrotizing enterocolitis (NEC) affects up to 10% of extremely-low-birthweight infants, with a 30% mortality rate. Currently, no biomarker reliably facilitates early diagnosis. Since thrombocytopenia and bowel ischemia are consistent findings in advanced NEC, we prospectively investigated two potential biomarkers: reticulated platelets (RP) and intestinal alkaline phosphatase (iAP). METHODS: Infants born ≤ 32 weeks and/or ≤ 1500 g were prospectively enrolled from 2009 to 2012...
February 2014: Journal of Pediatric Surgery
Hugh Simon Lam, Hon Ming Cheung, Terence Chuen Wai Poon, Raymond Pui On Wong, Kam Tong Leung, Karen Li, Pak Cheung Ng
BACKGROUND: Early detection and treatment of infected preterm infants could decrease morbidity and mortality. Neutrophil CD64 has been shown to be an excellent early diagnostic biomarker of late-onset sepsis (LOS) and necrotizing enterocolitis (NEC). We aimed to study whether using CD64 as a daily surveillance biomarker could predict LOS/NEC before clinical manifestation. METHODS: We collected 0.1 mL whole blood from very low birth weight (VLBW) infants from day 7 postnatal age until routine daily blood tests were no longer required...
December 2013: Clinical Chemistry
Thomas Benkoe, Carlos Reck, Andreas Gleiss, Stephan Kettner, Andreas Repa, Ernst Horcher, Winfried Rebhandl
PURPOSE: The aim of this study was to test the predictive value of interleukin (IL) 8 in the assessment of intestinal involvement in necrotizing enterocolitis (NEC). METHODS: Forty infants with surgically treated NEC were classified into 3 groups based on intestinal involvement during laparotomy: focal (n = 11), multifocal (n = 16), and panintestinal (n = 13). Preoperatively obtained serum levels of IL-8, C-reactive protein, white blood cell count, and platelet count were correlated with intestinal involvement using logistic regression models...
August 2012: Journal of Pediatric Surgery
Serafina Perrone, Maria Luisa Tataranno, Simona Negro, Sara Cornacchione, Mariangela Longini, Fabrizio Proietti, Vasso Soubasi, Manon J Benders, Frank Van Bel, Giuseppe Buonocore
INTRODUCTION: Oxidative stress (OS) is strongly involved in the pathogenesis of many preterm newborn diseases; this is due to the low efficiency of neonatal antioxidant systems unable to counteract the harmful effects of free radicals (FRs). Hypoxic-ischemic events and inflammation, involved in necrotizing enterocolitis (NEC) pathogenesis, are responsible of the overproduction of FRs, generating OS. AIM: To test the hypotesis that OS markers levels in cord blood may early identify the newborns at high risk to develop NEC...
April 2012: Journal of Maternal-fetal & Neonatal Medicine
Josef Cortez, Meenakshi Gupta, Arun Amaram, Janet Pizzino, Megha Sawhney, Beena G Sood
BACKGROUND: Diagnosis of necrotizing enterocolitis (NEC) in preterm neonates is challenging. We hypothesized that regional splanchnic oxygen saturation (rsSO₂) measured by near-infrared spectroscopy (NIRS) is a biomarker for mesenteric perfusion. OBJECTIVE: To evaluate feasibility and safety of continuous rsSO₂ monitoring in preterm infants in the first 14 days of life. METHODS: Preterm neonates ≤30 weeks' gestation had a NIRS sensor placed in the left paraumbilical region within 48 h of birth...
April 2011: Journal of Maternal-fetal & Neonatal Medicine
Pak Cheung Ng, Irene Ling Ang, Rossa Wai Kwun Chiu, Karen Li, Hugh Simon Lam, Raymond Pui On Wong, Kit Man Chui, Hon Ming Cheung, Eddy Wing Yin Ng, Tai Fai Fok, Joseph Jao Yiu Sung, Yuk Ming Dennis Lo, Terence Chuen Wai Poon
Preterm infants are highly susceptible to life-threatening infections that are clinically difficult to detect, such as late-onset septicemia and necrotizing enterocolitis (NEC). Here, we used a proteomic approach to identify biomarkers for diagnosis of these devastating conditions. In a case-control study comprising 77 sepsis/NEC and 77 nonsepsis cases (10 in each group being monitored longitudinally), plasma samples collected at clinical presentation were assessed in the biomarker discovery and independent validation phases...
August 2010: Journal of Clinical Investigation
Hyman M Schipper, C Shing Kwok, Scott M Rosendahl, Dirk Bandilla, Olivier Maes, Calvin Melmed, Daniel Rabinovitch, David H Burns
BACKGROUND: No established chemical biomarkers of idiopathic Parkinson's disease (PD) currently exist. Augmented oxidative stress (OS) has been implicated in both neural and peripheral PD tissues. METHODS: In this study, Raman scattering and near-infrared spectroscopy were used to detect and quantify oxidative substrate modifications in blood plasma samples from PD and normal elderly control (NEC) subjects. RESULTS: Hypothesis-driven preselection of OS-sensitive bandwidths distinguished PD from NEC subjects with approximately 75% sensitivity and specificity using both complementary spectroscopic techniques...
June 2008: Biomarkers in Medicine
David H Burns, Scott Rosendahl, Dirk Bandilla, Olivier C Maes, Howard M Chertkow, Hyman M Schipper
There are currently no accepted blood-based biomarkers of sporadic Alzheimer's disease (AD). Augmented oxidative stress has been implicated in both neural and peripheral AD tissues. In this study, we determined whether short-wavelength near-infrared (NIR) spectrophotometry of blood plasma differentiates mild sporadic AD from normal aging. NIR analysis was conducted on 75 microl plasma samples from 19 AD, 27 amnestic MCI, and 17 normal elderly control (NEC) persons using an optical fiber-coupled, holographic grating-based NIR spectrograph...
2009: Journal of Alzheimer's Disease: JAD
Christopher Young, Renu Sharma, Martin Handfield, Volker Mai, Josef Neu
Necrotizing enterocolitis (NEC) is the most common severe gastrointestinal emergency that affects premature newborns. This disease often has a rapid onset with few, if any, antecedent signs that can be used to reliably predict its occurrence. Its rapid onset and progression to death, as well as its severe morbidity when the infant survives, begs for early diagnostic tools that may be used in determining those infants who would be at greatest risk for development of the disease and for whom early preventative measures could be targeted...
May 2009: Pediatric Research
Ashima Madan, George El-Ferzli, Scott M Carlson, John C Whitin, James Schilling, Amir Najmi, Tom To-Sang Yu, Kenneth Lau, Reed A Dimmitt, Harvey J Cohen
Preterm infants are at risk of developing sepsis, necrotizing enterocolitis (NEC), chronic lung disease (CLD), and retinopathy of prematurity (ROP). We used high-throughput mass spectrometry to investigate whether cord blood proteins can be used to predict development of these morbidities. Cord blood plasma from 44 infants with a birth weight of <1500 g was analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF). Six infants developed ROP >or=stage II, 10 CLD, three sepsis, and one NEC...
February 2007: Pediatric Research
H M Schipper, H Chertkow, K Mehindate, D Frankel, C Melmed, H Bergman
BACKGROUND: Heme oxygenase-1 (HO-1) is a 32-kDa stress protein that catalyzes the degradation of heme to biliverdin. HO-1 immunoreactivity is greatly increased in neurons and astrocytes of the hippocampus and cerebral cortex of individuals with AD and colocalizes to senile plaques and neurofibrillary tangles. METHODS: We investigated whether systemic HO-1 regulation is also deranged in AD patients and whether blood HO-1 measurements provide a peripheral biomarker of the disease...
March 28, 2000: Neurology
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