keyword
https://read.qxmd.com/read/38463949/co-registration-of-maldi-msi-and-histology-demonstrates-gangliosides-co-localize-with-amyloid-beta-plaques-in-alzheimer-s-disease
#21
Nikita Ollen-Bittle, Shervin Pejhan, Stephen H Pasternak, C Dirk Keene, Qi Zhang, Shawn N Whitehead
Alzheimer's disease (AD) is a progressive neurological condition characterized by impaired cognitive function and behavioural alterations. While AD research historically centered around mis-folded proteins, advances in mass spectrometry techniques have triggered increased exploration of the AD lipidome with lipid dysregulation emerging as a critical player in AD pathogenesis. Gangliosides are a class of glycosphingolipids enriched within the central nervous system. Previous work has suggested a shift in a-series gangliosides from complex (GM1) to simple (GM2 and GM3) species may be related to the development of neurodegenerative disease...
February 27, 2024: Research Square
https://read.qxmd.com/read/38459207/clinical-advances-in-analytical-profiling-of-signature-lipids-implications-for-severe-non-communicable-and-neurodegenerative-diseases
#22
REVIEW
Sutanu Sarkar, Deotima Roy, Bhaskar Chatterjee, Rajgourab Ghosh
BACKGROUND: Lipids play key roles in numerous biological processes, including energy storage, cell membrane structure, signaling, immune responses, and homeostasis, making lipidomics a vital branch of metabolomics that analyzes and characterizes a wide range of lipid classes. Addressing the complex etiology, age-related risk, progression, inflammation, and research overlap in conditions like Alzheimer's Disease, Parkinson's Disease, Cardiovascular Diseases, and Cancer poses significant challenges in the quest for effective therapeutic targets, improved diagnostic markers, and advanced treatments...
March 8, 2024: Metabolomics: Official Journal of the Metabolomic Society
https://read.qxmd.com/read/38456419/effects-of-sex-and-western-diet-on-spatial-lipidomic-profiles-for-the-hippocampus-cortex-and-corpus-callosum-in-mice-using-maldi-msi
#23
JOURNAL ARTICLE
Catelynn C Shafer, Jacopo Di Lucente, Ulises Ruiz Mendiola, Izumi Maezawa, Lee-Way Jin, Elizabeth K Neumann
Diet is inextricably linked to human health and biological functionality. Reduced cognitive function among other health issues has been correlated with a western diet (WD) in mouse models, indicating that increases in neurodegeneration could be fueled in part by a poor diet. In this study, we use matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to spatially map the lipidomic profiles of male and female mice that were fed a high-fat, high-sucrose WD for a period of 7 weeks. Our findings concluded that the cortex and corpus callosum showed significant lipid variation by WD in female mice, while there was little to no variation in the hippocampus, regardless of sex...
March 8, 2024: Journal of the American Society for Mass Spectrometry
https://read.qxmd.com/read/38456255/a-statistical-approach-to-system-suitability-testing-for-mass-spectrometry-imaging
#24
JOURNAL ARTICLE
Alexandria L Sohn, Russell R Kibbe, Olivia E Dioli, Emily C Hector, Hongxia Bai, Kenneth P Garrard, David C Muddiman
RATIONALE: Mass spectrometry imaging (MSI) elevates the power of conventional mass spectrometry (MS) to multidimensional space, elucidating both chemical composition and localization. However, the field lacks any robust quality control (QC) and/or system suitability testing (SST) protocols to monitor inconsistencies during data acquisition, both of which are integral to ensure the validity of experimental results. To satisfy this demand in the community, we propose an adaptable QC/SST approach with five analyte options amendable to various ionization MSI platforms (e...
May 15, 2024: Rapid Communications in Mass Spectrometry: RCM
https://read.qxmd.com/read/38451276/visualization-of-pfoa-accumulation-and-its-effects-on-phospholipid-in-zebrafish-liver-by-maldi-imaging
#25
JOURNAL ARTICLE
Qiuyue Shi, Xian Zhang, Xiaohui Liu, Changzhou Yan, Shaoyong Lu
Exposure to poly- and perfluoroalkyl substances (PFASs) can result in bioaccumulation. Initial findings suggested that PFASs could accumulate in tissues rich in both phospholipids and proteins. However, our current understanding is limited to the average concentration of PFASs or phospholipid content across entire tissue matrices, leaving unresolved the spatial variations of lipid metabolism associated with PFOA in zebrafish tissue. To address gap, we developed a novel methodology for concurrent spatial profiling of perfluorooctanoic acid (PFOA) and individual phospholipids within zebrafish hepatic tissue sections, utilizing matrix-assisted laser desorption/ionization time of flight imaging mass spectrometry (MALDI-TOF-MSI)...
March 7, 2024: Analytical and Bioanalytical Chemistry
https://read.qxmd.com/read/38445816/spatial-neurolipidomics-maldi-mass-spectrometry-imaging-of-lipids-in-brain-pathologies
#26
REVIEW
Durga Jha, Kaj Blennow, Henrik Zetterberg, Jeffrey N Savas, Jörg Hanrieder
Given the complexity of nervous tissues, understanding neurochemical pathophysiology puts high demands on bioanalytical techniques with respect to specificity and sensitivity. Mass spectrometry imaging (MSI) has evolved to become an important, biochemical imaging technology for spatial biology in biological and translational research. The technique facilitates comprehensive, sensitive elucidation of the spatial distribution patterns of drugs, lipids, peptides, and small proteins in situ. Matrix-assisted laser desorption ionization (MALDI)-based MSI is the dominating modality due to its broad applicability and fair compromise of selectivity, sensitivity price, throughput, and ease of use...
March 2024: Journal of Mass Spectrometry: JMS
https://read.qxmd.com/read/38438227/towards-developing-a-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging-maldi-msi-compatible-tissue-expansion-protocol
#27
JOURNAL ARTICLE
Li-Cyun Chen, Chuping Lee, Cheng-Chih Hsu
Mass spectrometry imaging (MSI) visualizes spatial distribution of molecules in a biological tissue. However, compared with traditional microscopy-based imaging, conventional MSI is limited to its spatial resolution, resulting in difficulties in identifying detailed tissue morphological characters, such as lesion boundary or nanoscale structures. On the other hand, expansion microscopy, a tissue expansion method widely used in optical imaging to improve morphological details, has great potential to solve insufficient spatial resolution in mass spectrometry imaging (MSI)...
April 8, 2024: Analytica Chimica Acta
https://read.qxmd.com/read/38435075/recent-developments-and-application-of-mass-spectrometry-imaging-in-n-glycosylation-studies-an-overview
#28
REVIEW
Bharath S Kumar
Among the most typical posttranslational modifications is glycosylation, which often involves the covalent binding of an oligosaccharide (glycan) to either an asparagine (N-linked) or a serine/threonine (O-linked) residue. Studies imply that the N-glycan portion of a glycoprotein could serve as a particular disease biomarker rather than the protein itself because N-linked glycans have been widely recognized to evolve with the advancement of tumors and other diseases. N-glycans found on protein asparagine sites have been especially significant...
2024: Mass Spectrometry
https://read.qxmd.com/read/38430431/biofortified-rice-provides-rich-sakuranetin-in-endosperm
#29
JOURNAL ARTICLE
Yao Zhao, Jitao Hu, Zhongjing Zhou, Linying Li, Xueying Zhang, Yuqing He, Chi Zhang, Junmin Wang, Gaojie Hong
Sakuranetin plays a key role as a phytoalexin in plant resistance to biotic and abiotic stresses, and possesses diverse health-promoting benefits. However, mature rice seeds do not contain detectable levels of sakuranetin. In the present study, a transgenic rice plant was developed in which the promoter of an endosperm-specific glutelin gene OsGluD-1 drives the expression of a specific enzyme naringenin 7-O-methyltransferase (NOMT) for sakuranetin biosynthesis. The presence of naringenin, which serves as the biosynthetic precursor of sakuranetin made this modification feasible in theory...
March 2, 2024: Rice
https://read.qxmd.com/read/38427328/mass-spectrometry-imaging-and-histology-for-the-analysis-of-budding-intestinal-organoids
#30
JOURNAL ARTICLE
Emily R Sekera, Kubra B Akkaya-Colak, Arbil Lopez, Maria M Mihaylova, Amanda B Hummon
Three-dimensional (3D) organoids have been at the forefront of regenerative medicine and cancer biology fields for the past decade. However, the fragile nature of organoids makes their spatial analysis challenging due to their budding structures and composition of single layer of cells. The standard sample preparation approaches can collapse the organoid morphology. Therefore, in this study, we evaluated several approaches to optimize a method compatible with both mass spectrometry imaging (MSI) and immunohistological techniques...
March 1, 2024: Analytical Chemistry
https://read.qxmd.com/read/38407924/a-machine-learning-driven-comparison-of-ion-images-obtained-by-maldi-and-maldi-2-mass-spectrometry-imaging
#31
JOURNAL ARTICLE
Tassiani Sarretto, Wil Gardner, Daniel Brungs, Sarbar Napaki, Paul J Pigram, Shane R Ellis
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) enables label-free imaging of biomolecules in biological tissues. However, many species remain undetected due to their poor ionization efficiencies. MALDI-2 (laser-induced post-ionization) is the most widely used post-ionization method for improving analyte ionization efficiencies. Mass spectra acquired using MALDI-2 constitute a combination of ions generated by both MALDI and MALDI-2 processes. Until now, no studies have focused on a detailed comparison between the ion images (as opposed to the generated m/z values) produced by MALDI and MALDI-2 for mass spectrometry imaging (MSI) experiments...
February 26, 2024: Journal of the American Society for Mass Spectrometry
https://read.qxmd.com/read/38406559/optimized-combination-of-maldi-msi-and-immunofluorescence-for-neuroimaging-of-lipids-within-cellular-microenvironments
#32
JOURNAL ARTICLE
Catelynn C Shafer, Elizabeth K Neumann
Proper neurological function relies on the cellular and molecular microenvironment of the brain, with perturbations of this environment leading to neurological disorders. However, studying the microenvironments of neurological tissue has proven difficult because of its inherent complexity. Both the cell type and metabolomic underpinnings of the cell have crucial functional roles, thus making multimodal characterization methods key to acquiring a holistic view of the brain's microenvironment. This study investigates methods for combining matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) and immunofluorescence (IF) microscopy to enable the concurrent investigation of cell types and lipid profiles on the same sample...
2024: Frontiers in Chemistry
https://read.qxmd.com/read/38402423/advancing-rare-cancer-research-by-maldi-mass-spectrometry-imaging-applications-challenges-and-future-perspectives-in-sarcoma
#33
REVIEW
Maren Nicole Stillger, Mujia Jenny Li, Pia Hönscheid, Cläre von Neubeck, Melanie Christine Föll
MALDI mass spectrometry imaging (MALDI imaging) uniquely advances cancer research, by measuring spatial distribution of endogenous and exogenous molecules directly from tissue sections. These molecular maps provide valuable insights into basic and translational cancer research, including tumor biology, tumor microenvironment, biomarker identification, drug treatment, and patient stratification. Despite its advantages, MALDI imaging is underutilized in studying rare cancers. Sarcomas, a group of malignant mesenchymal tumors, pose unique challenges in medical research due to their complex heterogeneity and low incidence, resulting in understudied subtypes with suboptimal management and outcomes...
February 25, 2024: Proteomics
https://read.qxmd.com/read/38384722/tissue-microarray-lipidomic-imaging-mass-spectrometry-method-application-to-the-study-of-alcohol-related-white-matter-neurodegeneration
#34
JOURNAL ARTICLE
Isabel Gameiro-Ros, Lelia Noble, Ming Tong, Emine B Yalcin, Suzanne M de la Monte
Central nervous system (CNS) white matter pathologies accompany many diseases across the lifespan, yet their biochemical bases, mechanisms, and consequences have remained poorly understood due to the complexity of myelin lipid-based research. However, recent advances in matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) have minimized or eliminated many technical challenges that previously limited progress in CNS disease-based lipidomic research. MALDI-IMS can be used for lipid identification, semi-quantification, and the refined interpretation of histopathology...
June 2023: Appl Biosci (Basel)
https://read.qxmd.com/read/38376352/investigating-the-effects-of-pfoa-accumulation-and-depuration-on-specific-phospholipids-in-zebrafish-through-imaging-mass-spectrometry
#35
JOURNAL ARTICLE
Qiuyue Shi, Zhengfen Wan, Shaoyong Lu, Cheng Fang, Changzhou Yan, Xian Zhang
Perfluorooctanoic acid (PFOA) is an emerging persistent organic pollutant. Exposure to PFOA was observed to have a correlation with the expression levels of phospholipids. However, there are currently no studies that directly visualize the effects of PFOA on phospholipids. To this end, matrix-assisted laser desorption/ionization time of flight imaging mass spectrometry (MALDI-TOF-IMS) was used to visualize changes in phospholipids in the different tissues of zebrafish following exposure to PFOA. This study found that the major perturbed phospholipids were phosphatidylcholine (PC), diacylglycerol (DG), phosphatidic acid (PA), phosphatidylglycerol (PG), sphingomyelin (SM), and triacylglycerol (TG)...
February 20, 2024: Environmental Science. Processes & Impacts
https://read.qxmd.com/read/38356087/thin-section-and-matrix-free-mass-spectrometry-imaging-reproducible-sample-transfer-using-novel-platinum-coated-porous-plate-formed-of-glass-beads
#36
JOURNAL ARTICLE
Takamasa Ikeda, Masahiro Kotani
RATIONALE: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) typically requires sample preparation such as sectioning and spraying of the matrix. Sample transfer using a Pt-coated porous plate formed of glass beads simplifies preparation and enables reproducible MSI measurements. METHODS: The surface of a sintered-glass-bead porous plate was coated with Pt on one side of the plate. Polymer additives and the cross-section of a strawberry were chosen as the sample and transferred to the Pt-coated surface of the plate...
March 30, 2024: Rapid Communications in Mass Spectrometry: RCM
https://read.qxmd.com/read/38345665/assessing-molecular-localization-of-symbiont-microalgae-in-coral-branches-through-mass-spectrometry-imaging
#37
JOURNAL ARTICLE
Shudai Sasaki, Tsukasa Mori, Hirofumi Enomoto, Sakiko Nakamura, Hideo Yokota, Hiroshi Yamashita, Naoko Goto-Inoue
Reef-building corals are a fundamental pillar of coral reef ecosystems in tropical and subtropical shallow environments. Corals harbor symbiotic dinoflagellates belonging to the family Symbiodiniaceae, commonly known as zooxanthellae. Extensive research has been conducted on this symbiotic relationship, yet the fundamental information about the distribution and localization of Symbiodiniaceae cells in corals is still limited. This information is crucial to understanding the mechanism underlying the metabolite exchange between corals and their algal symbionts, as well as the metabolic flow within holobionts...
February 12, 2024: Marine Biotechnology
https://read.qxmd.com/read/38330048/maldi-tof-imaging-analysis-of-benzalkonium-chloride-penetration-in-ex-vivo-human-skin
#38
JOURNAL ARTICLE
Chase N Morse, Collin C Hite, Nathan C Wamer, Jennifer N Gadient, Gabriella Baki, Erin G Prestwich
Benzalkonium chloride (BZK), alkyldimethylbenzlamonium chloride, is a cationic surfactant that is used as an antiseptic. BZK is classified as a quaternary ammonium compound composed of molecules of several alkyl chains of differing lengths, that dictate its effectiveness towards different microbes. As a result, BZK has become one of the most used preservatives in antibacterial solutions. Despite its widespread use, it is not clear whether BZK penetrates human skin. To answer this question, BZK treated skin was analyzed using matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry imaging...
2024: PloS One
https://read.qxmd.com/read/38324070/spatial-ms-multiomics-on-clinical-prostate-cancer-tissues
#39
JOURNAL ARTICLE
Jacob X M Truong, Sushma R Rao, Feargal J Ryan, David J Lynn, Marten F Snel, Lisa M Butler, Paul J Trim
Mass spectrometry (MS) and MS imaging (MSI) are used extensively for both the spatial and bulk characterization of samples in lipidomics and proteomics workflows. These datasets are typically generated independently due to different requirements for sample preparation. However, modern omics technologies now provide higher sample throughput and deeper molecular coverage, which, in combination with more sophisticated bioinformatic and statistical pipelines, make generating multiomics data from a single sample a reality...
February 7, 2024: Analytical and Bioanalytical Chemistry
https://read.qxmd.com/read/38319357/assessment-of-maldi-matrices-for-the-detection-and-visualization-of-phosphatidylinositols-and-phosphoinositides-in-mouse-kidneys-through-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging-maldi-msi
#40
JOURNAL ARTICLE
Laurentiu G Dabija, Maryam Yousefi-Taemeh, Ergi Duli, Mathieu Lemaire, Demian R Ifa
Phosphatidylinositols and their phosphorylated derivatives, known as phosphoinositides, are crucial in cellular processes, with their abnormalities linked to various diseases. Thus, identifying and measuring phosphoinositide levels in tissues are crucial for understanding their contributions to cellular processes and disease development. One powerful technique for mapping the spatial distribution of molecules in biological samples is matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)...
February 6, 2024: Analytical and Bioanalytical Chemistry
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