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MALDI Imaging

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https://www.readbyqxmd.com/read/28446534/proteomics-analysis-of-tissue-samples-reveals-changes-in-mitochondrial-protein-levels-in-parathyroid-hyperplasia-over-adenoma
#1
Gurler Akpinar, Murat Kasap, Nuh Zafer Canturk, Mehin Zulfigarova, Eylül Ece Islek, Sertac Ata Guler, Turgay Simsek, Zeynep Canturk
BACKGROUND/AIM: To unveil the pathophysiology of primary hyperparathyroidism, molecular details of parathyroid hyperplasia and adenoma have to be revealed. Such details will provide the tools necessary for differentiation of these two look-alike diseases. Therefore, in the present study, a comparative proteomic study using postoperative tissue samples from the parathyroid adenoma and parathyroid hyperplasia patients was performed. MATERIALS AND METHODS: Protein extracts were prepared from tissue samples (n=8 per group)...
May 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28439630/myriocin-treatment-of-cf-lung-infection-and-inflammation-complex-analyses-for-enigmatic-lipids
#2
Anna Caretti, Michele Vasso, Fabiola Tecla Bonezzi, Andrea Gallina, Marco Trinchera, Alice Rossi, Raffaella Adami, Josefina Casas, Monica Falleni, Delfina Tosi, Alessandra Bragonzi, Riccardo Ghidoni, Cecilia Gelfi, Paola Signorelli
Our aim was to use quantitative and qualitative analyses to gain further insight into the role of ceramide in cystic fibrosis (CF). Sphingolipid ceramide is a known inflammatory mediator, and its accumulation in inflamed lung has been reported in different types of emphysema, chronic obstructive pulmonary disease and CF. CF is caused by a mutation of the chloride channel and associated with hyperinflammation of the respiratory airways and high susceptibility to ongoing infections. We have previously demonstrated that de novo ceramide synthesis is enhanced in lung inflammation and sustains Pseudomonas aeruginosa pulmonary infection in a CF murine model...
April 24, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28438696/altered-temporal-lobe-white-matter-lipid-ion-profiles-in-an-experimental-model-of-sporadic-alzheimer-s-disease
#3
Ming Tong, Raiane Leão, Gina V Vimbela, Emine B Yalcin, Jared Kay, Alexander Krotow, Suzanne M de la Monte
BACKGROUND: White matter is an early and important yet under-evaluated target of Alzheimer's disease (AD). Metabolic impairments due to insulin and insulin-like growth factor resistance contribute to white matter degeneration because corresponding signal transduction pathways maintain oligodendrocyte function and survival. METHODS: This study utilized a model of sporadic AD in which adult Long Evans rats administered intracerebral streptozotocin (i.c. STZ) developed AD-type neurodegeneration...
April 21, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28437719/matrix-assisted-laser-desorption-ionization-mass-spectrometric-imaging-of-olanzapine-in-a-single-hair-using-esculetin-as-a-matrix
#4
Hang Wang, Ying Wang, Ge Wang, Lizhi Hong
Matrix-assisted laser desorption/ionization-mass spectrometric imaging (MALDI-MSI) for the analysis of intact hair is a powerful tool for monitoring changes in drug consumption. The embedding of a low drug concentration in the hydrophobic hair matrix makes it difficult to extract and detect, and requires an improved method to increase detection sensitivity. In this study, an MSI method using MALDI-Fourier transform ion cyclotron resonance was developed for direct identification and imaging of olanzapine in hair samples using the positive ion mode...
April 18, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28436032/application-of-maldi-tof-ms-for-estimating-the-postmortem-interval-in-rat-muscle-samples
#5
Chengzhi Li, Dong Ma, Kaifei Deng, Yijiu Chen, Ping Huang, Zhenyuan Wang
Estimating the postmortem interval (PMI) is very important in the forensic sciences. Although many approaches have been used for estimating the PMI, accurate PMI calculations are still difficult. In this study, four Sprague Dawley (SD) rats were sacrificed by suffocation, and muscle samples were collected by dissection at various time intervals (0, 48, 96, and 144 h) after death. All samples were probed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to obtain molecular images and data for principal component analysis (PCA)...
April 23, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28411106/mass-spectrometry-imaging-shows-major-derangements-in-neurogranin-and-in-purine-metabolism-in-the-triple-knockout-3%C3%A3-tg-alzheimer-mouse-model
#6
Clara Esteve, Emrys A Jones, Douglas B Kell, Hervé Boutin, Liam A McDonnell
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) can simultaneously measure hundreds of biomolecules directly from tissue. Using different sample preparation strategies, proteins and metabolites have been profiled to study the molecular changes in a 3×Tg mouse model of Alzheimer's disease. In comparison with wild-type (WT) control mice MALDI-MSI revealed Alzheimer's disease-specific protein profiles, highlighting dramatic reductions of a protein with m/z 7560, which was assigned to neurogranin and validated by immunohistochemistry...
April 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28409937/spatial-and-temporal-localization-of-flavonoid-metabolites-in-strawberry-fruit-fragaria-%C3%A3-ananassa
#7
Anna C Crecelius, Dirk Hölscher, Thomas Hoffmann, Bernd Schneider, Thilo C Fischer, Magda-Viola Hanke, Henryk Flachowsky, Wilfried Schwab, Ulrich S Schubert
Flavonoids are important metabolites in strawberries (Fragaria × ananassa) because they accomplish an extensive collection of physiological functions and are valuable for human health. However, their localization within the fruit tissue has not been extensively explored. Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to shed light on the spatial distribution of flavonoids during fruit development. One wild-type (WT) and two transgenic lines were compared, wherein the transgenic enzymes anthocyanidin reductase (ANRi) and flavonol synthase (FLSi), respectively, were down-regulated using an RNAi-based silencing approach...
April 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28401432/feasibility-assessment-of-a-maldi-fticr-imaging-approach-for-the-3d-reconstruction-of-a-mouse-lung
#8
E Ellen Jones, Cristine Quiason, Stephanie Dale, Sheerin K Shahidi-Latham
Matrix assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) has proven to be a quick, robust, and label-free tool to produce two-dimensional (2D) ion-density maps representing the distribution of a variety of analytes across a tissue section of interest. In addition, three-dimensional (3D) imaging mass spectrometry workflows have been developed that are capable of visualizing these same analytes throughout an entire volume of a tissue rather than a single cross-section. Until recently, the use of Fourier transform ion cyclotron resonance (FTICR) mass spectrometers for 3D volume reconstruction has been impractical due to software limitations, such as inadequate capacity to manipulate the extremely large data files produced during an imaging experiment...
April 11, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28396286/altered-mitochondrial-and-peroxisomal-integrity-in-lipocalin-2-deficient-mice-with-hepatic-steatosis
#9
Anastasia Asimakopoulou, Annabelle Fülöp, Erawan Borkham-Kamphorst, Eddy Van de Leur, Nikolaus Gassler, Thorsten Berger, Birte Beine, Helmut E Meyer, Tak W Mak, Carsten Hopf, Corinna Henkel, Ralf Weiskirchen
Lipocalin-2 (LCN2) is a secreted adipokine that transports small hydrophobic molecules such as fatty acids and steroids. LCN2 limits bacterial growth by sequestering iron-containing siderophores and in mammalian liver protects against inflammation, infection, injury and other stressors. Because LCN2 modulates hepatic fat metabolism and homeostasis, we performed a comparative profiling of proteins and lipids of wild type (WT) and Lcn2-deficient mice fed either standard chow or a methionine- and choline-deficient (MCD) diet...
April 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28387396/implementation-of-maldi-ms-profiling-and-imaging-methods-for-the-analysis-of-real-crime-scene-fingermarks
#10
R Bradshaw, N Denison, S Francese
In the study presented here, four examples of crime scene fingermarks analysed by Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI MSI), in collaboration with the local Police Force, are reported. These marks are associated to high profile crimes such as drug dealing, murder and harassment. Following the application of forensic enhancement techniques by the CSI or the crime lab, one of the marks could be directly analysed on the surface of deposition and the others were analysed as primary lifts...
April 7, 2017: Analyst
https://www.readbyqxmd.com/read/28385670/spatial-profiling-of-maytansine-during-the-germination-process-of-maytenus-senegalensis-seeds
#11
Dennis Eckelmann, Souvik Kusari, Michael Spiteller
The ecological role of maytansine, an important antineoplastic and antimicrobial compound with high cytotoxicity, particularly as a chemical defense compound has remained elusive since its discovery in the 1970s in Maytenus and Putterlickia plants. In the present study, we have used MALDI-imaging-HRMS to visualize the occurrence as well as spatial and temporal distribution of maytansine in a Maytenus senegalensis plant, seeds obtained from the mother plant during seeding stage, through the germination of the seeds, and finally up to the establishment of seedlings (or daughter plants)...
April 3, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28377615/ultra-high-mass-resolution-maldi-imaging-mass-spectrometry-of-proteins-and-metabolites-in-a-mouse-model-of-glioblastoma
#12
M Dilillo, R Ait-Belkacem, C Esteve, D Pellegrini, S Nicolardi, M Costa, E Vannini, E L de Graaf, M Caleo, L A McDonnell
MALDI mass spectrometry imaging is able to simultaneously determine the spatial distribution of hundreds of molecules directly from tissue sections, without labeling and without prior knowledge. Ultra-high mass resolution measurements based on Fourier-transform mass spectrometry have been utilized to resolve isobaric lipids, metabolites and tryptic peptides. Here we demonstrate the potential of 15T MALDI-FTICR MSI for molecular pathology in a mouse model of high-grade glioma. The high mass accuracy and resolving power of high field FTICR MSI enabled tumor specific proteoforms, and tumor-specific proteins with overlapping and isobaric isotopic distributions to be clearly resolved...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28365985/two-dimensional-correlation-spectroscopy-for-multimodal-analysis-of-ft-ir-raman-and-maldi-tof-ms-hyperspectral-images-with-hamster-brain-tissue
#13
Peter Lasch, Isao Noda
Hyperspectral imaging (HSI) techniques are useful for obtaining very detailed structural and compositional information from biomedical, pharmaceutical, or clinical samples, among others. The informative value of these methods can be further increased through the application of different HSI techniques and joint analysis of the data. However, interpretation and understanding of multimodal HSI have been impeded by difficulties in registration of the different HSI data sets and by the lack of integrative analysis concepts...
April 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28362367/sublimation-of-dan-matrix-for-the-detection-and-visualization-of-gangliosides-in-rat-brain-tissue-for-maldi-imaging-mass-spectrometry
#14
Sarah Caughlin, Dae Hee Park, Ken K-C Yeung, David F Cechetto, Shawn N Whitehead
Sample preparation is key for optimal detection and visualization of analytes in Matrix-assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS) experiments. Determining the appropriate protocol to follow throughout the sample preparation process can be difficult as each step must be optimized to comply with the unique characteristics of the analytes of interest. This process involves not only finding a compatible matrix that can desorb and ionize the molecules of interest efficiently, but also selecting the appropriate matrix deposition technique...
March 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28361385/maldi-orbitrap-mass-spectrometry-profiling-of-dysregulated-sulfoglycosphingolipids-in-renal-cell-carcinoma-tissues
#15
Robert Jirásko, Michal Holčapek, Maria Khalikova, David Vrána, Vladimír Študent, Zuzana Prouzová, Bohuslav Melichar
Matrix-assisted laser desorption/ionization coupled with Orbitrap mass spectrometry (MALDI-Orbitrap-MS) is used for the clinical study of patients with renal cell carcinoma (RCC), as the most common type of kidney cancer. Significant changes in sulfoglycosphingolipid abundances between tumor and autologous normal kidney tissues are observed. First, sulfoglycosphingolipid species in studied RCC samples are identified using high mass accuracy full scan and tandem mass spectra. Subsequently, optimization, method validation, and statistical evaluation of MALDI-MS data for 158 tissues of 80 patients are discussed...
March 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28341601/advances-in-maldi-imaging-mass-spectrometry-of-proteins-in-cardiac-tissue-including-the-heart-valve
#16
REVIEW
Peggi M Angel, H Scott Baldwin, Danielle Gottlieb Sen, Yan Ru Su, John E Mayer, David Bichell, Richard R Drake
Significant progress has been made for tissue imaging of proteins using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). These advancements now facilitate mapping of a wide range of proteins, peptides, and post-translational modifications in a wide variety of tissues; however, the use of MALDI IMS to detect proteins from cardiac tissue is limited. This review discusses the most recent advances in protein imaging and demonstrates application to cardiac tissue, including the heart valve...
March 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28336905/a-nanostructured-matrices-assessment-to-study-drug-distribution-in-solid-tumor-tissues-by-mass-spectrometry-imaging
#17
Silvia Giordano, Valentina Pifferi, Lavinia Morosi, Melinda Morelli, Luigi Falciola, Giuseppe Cappelletti, Sonja Visentin, Simonetta A Licandro, Roberta Frapolli, Massimo Zucchetti, Roberta Pastorelli, Laura Brunelli, Maurizio D'Incalci, Enrico Davoli
The imaging of drugs inside tissues is pivotal in oncology to assess whether a drug reaches all cells in an adequate enough concentration to eradicate the tumor. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI) is one of the most promising imaging techniques that enables the simultaneous visualization of multiple compounds inside tissues. The choice of a suitable matrix constitutes a critical aspect during the development of a MALDI-MSI protocol since the matrix ionization efficiency changes depending on the analyte structure and its physico-chemical properties...
March 21, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28335978/systematic-assessment-of-surfactants-for-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
#18
Bijay Banstola, Eulalie T Grodner, Fan Cao, Fabrizio Donnarumma, Kermit K Murray
A systematic method for evaluation of MALDI profiling and imaging was developed and applied to the use of three surfactants, sodium dodecyl sulfate (SDS), Triton X-100, and Tween 20, on rat brain tissue. For profiling studies, mass spectra were acquired from regular arrays of spots with manually deposited surfactant and matrix. The studies recorded the total number of peaks in the mass spectra from 2 to 20 kDa and compared the number of peaks and peak intensities with and without surfactant. It was found that SDS decreases the total number of peaks at all concentrations but does lead to an increase in the number of peaks below 5 kDa...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28329870/application-of-imaging-techniques-to-cases-of-drug-induced-crystal-nephropathy-in-preclinical-studies
#19
B Lenz, A Brink, M Siam, A De Paepe, S Bassett, A Eichinger-Chapelon, P Maliver, R Neff, U Niederhauser, B Steinhuber, R Zurbach, T Singer, C Funk, F Schuler, M Albassam, S Schadt
A number of drugs can cause precipitates within renal tubules leading to crystal nephropathy. Crystal nephropathy is usually an exposure-related finding and is not uncommon in preclinical studies, where high doses are tested. An understanding of the nature of precipitates is important for human risk assessment and further development. Our aim was to investigate the ability of various imaging techniques to detect the presence of drugs or metabolites in renal crystals. We applied matrix-assisted laser desorption/ionization - Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS) imaging, Raman and infrared microspectroscopy, scanning electron microscopy coupled with energy dispersive X-ray (SEM/EDX) spectroscopy and standard histopathology to cases of drug-induced crystal nephropathy, induced in rodents and primates by four compounds...
February 27, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28325940/one-pot-synthesis-of-four-coordinate-boron-iii-complexes-by-the-ligand-promoted-organic-group-migration-between-boronic-acids
#20
Venkata S Sadu, Hye-Rin Bin, Do-Min Lee, Kee-In Lee
Multidisciplinary applications of four-coordinate boron(III) complexes make them very attractive and challenging research field in chemistry, biology and material sciences. The dual role played by boron atom in stabilising the chelate ligand and enhancing the π-conjugation makes them very useful as luminescent materials for organic electronics and photonics, and sensing and imaging probes for biomedical purposes. The conventional methods involve the use of diarylborinic acids or anhydrides and triaryl boranes, which are made from organometallic reagents...
March 21, 2017: Scientific Reports
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