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MALDI Imaging

Gabriela Miana de Mattos Paixão, Horácio Eduardo Veronesi, Halsted Alarcão Gomes Pereira da Silva, José Nunes de Alencar Neto, Carolina de Paulo Maldi, Luciano de Figueiredo Aguiar Filho, Ibrahim Masciarelli Francisco Pinto, Francisco Faustino de Albuquerque Carneiro de França, Edileide de Barros Correia
BACKGROUND: Electrocardiogram is the initial test in the investigation of heart disease. Electrocardiographic changes in hypertrophic cardiomyopathy have no set pattern, and correlates poorly with echocardiographic findings. Cardiac magnetic resonance imaging has been gaining momentum for better assessment of hypertrophy, as well as the detection of myocardial fibrosis. OBJECTIVES: To correlate the electrocardiographic changes with the location of hypertrophy in hypertrophic cardiomyopathy by cardiac magnetic resonance...
January 2018: Arquivos Brasileiros de Cardiologia
Célia Fernandes, Etelvina Figueira, Romà Tauler, Carmen Bedia
Chlorpyrifos (CPF) is a worldwide used pesticide that raises concerns from the environmental and human health perspectives. The presence of pesticides such as CPF in edible vegetables has been already reported, but little is known about the effects induced by this pesticide stress on the morphology, oxidative response and lipid composition of treated plants. In this work, green bean plants (Phaseolus vulgaris) were exposed to increasing concentrations of CPF and the different plant parts (roots, stem bases, stem, leaves, pods and beans) were subjected to different analyses...
March 7, 2018: Ecotoxicology and Environmental Safety
Giulio Calza, Elisabeth Nyberg, Matias Mäkinen, Rabah Soliymani, Annunziata Cascone, Dan Lindholm, Emanuele Barborini, Marc Baumann, Maciej Lalowski, Ove Eriksson
Metformin is the first line drug for type 2 diabetes but its molecular mechanisms remain unclear. Here, we have studied the acute effect of a therapeutically relevant intrahepatic concentration of metformin on glucose production from lactate. We selected the perfused rat liver as experimental system since it enables the complete control of drug dosage. We used MALDI (matrix-assisted laser desorption/ionization) mass spectrometry imaging to estimate the concentration of metformin in the livers and we measured the concentration of glucose in the effluent medium under basal conditions and following lactate addition...
2018: Frontiers in Pharmacology
Vishakha Pandey, Manoj Singh, Dinesh Pandey, Soma Marla, Anil Kumar
Tilletia indica is a smut fungus that incites Karnal bunt in wheat. It has been considered as quarantine pest in more than 70 countries. Despite its quarantine significance, there is meager knowledge regarding the molecular mechanisms of disease pathogenesis. Moreover, various disease management strategies have proven futile. Development of effective disease management strategy requires identification of pathogenicity/virulence factors. With this aim, the present study was conducted to compare the secretomes of T...
March 6, 2018: Proteomics
Anna Wojakowska, Laura M Cole, Mykola Chekan, Katarzyna Bednarczyk, Magdalena Maksymiak, Małgorzata Oczko-Wojciechowska, Barbara Jarząb, Malcolm R Clench, Joanna Polańska, Monika Pietrowska, Piotr Widlak
INTRODUCTION: The distinction of papillary thyroid carcinomas from benign thyroid lesions has important implication for clinical man-agement. Classification based on histopathological features can be supported by molecular biomarkers, including lipidomic signatures, identified with the use of high-throughput mass spectrometry techniques. Formalin fixation is a standard procedure for stabilization and preservation of tissue samples, therefore this type of samples constitute highly valuable source of clinical material for retrospective molecular studies...
2018: Endokrynologia Polska
Fiona Henderson, Philippa J Hart, Jesus M Pradillo, Michael Kassiou, Lidan Christie, Kaye J Williams, Herve Boutin, Adam McMahon
RATIONALE: Stroke is a leading cause of disability worldwide. Understanding the recovery process post-stroke is essential; however, longer-term recovery studies are lacking. In-vivo Positron emission tomography (PET) can image biological recovery processes, but is limited by spatial resolution and its targeted nature. Untargeted mass spectrometry imaging offers high spatial resolution, providing an ideal ex-vivo tool for brain recovery imaging. METHODS: Magnetic resonance imaging (MRI) was used to image a rat brain 48 hours after ischaemic stroke to locate the infarcted regions of the brain...
February 27, 2018: Rapid Communications in Mass Spectrometry: RCM
Alexander J Kim, Sankha Basu, Carolyn Glass, Edgar L Ross, Nathalie Agar, Qing He, David Calligaris
Opioids are often used for analgesia via continuous intrathecal delivery by implantable devices. Higher concentration and daily dose of opioid have been postulated as risk factors for intrathecal granuloma formation. We present a 42-year-old female patient with chronic abdominal pain from refractory pancreatitis, with an intrathecal drug delivery device implanted 21 years prior, delivering continuous intrathecal morphine. After many years without concerning physical signs or complaint, with gradual increases in daily morphine dose, she presented with rapidly progressive neurologic deficits, including lower extremity, bladder, and bowel symptoms...
February 26, 2018: Pain Practice: the Official Journal of World Institute of Pain
Teesha C Luehr, Emily M Koide, Xiaodong Wang, Jun Han, Christoph H Borchers, Caren C Helbing
Anuran metamorphosis involves the transformation of an aquatic tadpole into a juvenile frog. This process is completely dependent upon thyroid hormones (THs). Although much research has been focused on changes in gene expression programs during this postembryonic developmental period, transitions in the metabolic profiles are relatively poorly understood. Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI) is a technique that generates highly multiplexed mass spectra while retaining spatial location information on a thin tissue section...
February 19, 2018: General and Comparative Endocrinology
Eva Marxen, Liang Jin, Jette Jacobsen, Christian Janfelt, Birgitte Hyrup, Joseph A Nicolazzo
PURPOSE: The purpose of this study was to assess the effect of several chemical permeation enhancers on the buccal permeability of nicotine and to image the spatial distribution of nicotine in buccal mucosa with and without buccal permeation enhancers. METHODS: The impact of sodium taurodeoxycholate (STDC), sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and Azone® on the permeability of [3 H]-nicotine and [14 C]-mannitol (a paracellular marker) across porcine buccal mucosa was studied ex vivo in modified Ussing chambers...
February 21, 2018: Pharmaceutical Research
Adam Taylor, Alex Dexter, Josephine Bunch
In this study we have explored several aspects of regional analyte suppression in mass spectrometry imaging of a heterogeneous sample; transverse cryosections of mouse brain. Olanzapine was homogeneously coated across the section prior to DESI and MALDI mass spectrometry imaging. We employed the concept of a tissue extinction coefficient (TEC) to assess suppression of an analyte on tissue relative to its intensity in an off tissue region. We expanded the use of TEC, by first segmenting anatomical regions using graph-cuts clustering, and calculating a TEC for each cluster...
February 20, 2018: Analytical Chemistry
Benjamin Brokinkel, Sabrina Kröger, Volker Senner, Astrid Jeibmann, Uwe Karst, Walter Stummer
BACKGROUND: The advantages of 5-aminolevulinacid (5-ALA)-induced fluorescence-guided surgery in meningiomas are increasingly discussed. In this context, despite detectable tumor tissue in histopathologial analyses, no fluorescence was shown at the dura tail using the standard operating microscope. Thus, 5-ALA metabolism in this surgically important site remains unknown but needs to be elucidated when further evaluating indications of fluorescence-guided surgery in meningiomas. METHOD: We here present the spatially resolved identification of protoporphyrin IX (PpIX) in sphenoid ridge meningioma cryosections from a patient who underwent fluorescence-guided microsurgery using molecular imaging analysis by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS)...
February 15, 2018: Acta Neurochirurgica
Jenny Schröter, Annabelle Fülöp, Carsten Hopf, Jürgen Schiller
Unequivocal assignment of phospholipid peaks in complex mixtures is difficult if only the m/z values but no tandem mass spectrometry (MS/MS) data are available. This is usually the case for matrix-assisted laser/desorption ionization time-of-flight (MALDI-TOF) MS imaging experiments and the analysis has normally to be performed without prior separation. Another problem might be the often matrix-induced loss of one methyl group in phosphatidylcholine (PC) species, which makes them detectable as negative ions becoming isomers of some phosphatidylethanolamines (PEs)...
February 14, 2018: Analytical and Bioanalytical Chemistry
Boone M Prentice, Daniel Ryan, Raf Van de Plas, Richard M Caprioli, Jeffrey M Spraggins
The molecular identification of species of interest is an important part of an imaging mass spectrometry (IMS) experiment. The high resolution accurate mass capabilities of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) have recently been shown to facilitate the identification of proteins in matrix-assisted laser desorption/ionization (MALDI) IMS. However, these experiments are typically limited to proteins giving rise to ions of relatively low m/z due to difficulties transmitting and measuring large molecular weight ions of low charge states...
February 14, 2018: Analytical Chemistry
Stefanie Pleik, Bernhard Spengler, Dhaka Ram Bhandari, Steven Luhn, Thomas Schäfer, Dieter Urbach, Dieter Kirsch
In forensic science, reconstructing the timing of events occurring during a criminal offense is of great importance. In some cases, the time when particular evidence was left on a crime scene is a critical matter. The ability to estimate the fingerprint age would raise the evidentiary value of fingerprints tremendously. For this purpose the most promising approach is the analysis of changes in the chemical compositions of fingerprint residues in the course of aging. The focus of our study is the identification of human specific compounds in fingerprint residues, characterized by a significant aging behavior that could analytically be used for the age determination of fingerprints in future...
February 12, 2018: Analyst
Chao Zhao, Peisi Xie, Ting Yong, Hailin Wang, Arthur Chung, Zongwei Cai
With the continuous exposure of environmental pollutants in organisms, determination of abundance variation and spatial distribution of lipids might expand our understanding of toxicological mechanisms occurring in kidney. Herein, an integrated method involving mass spectrometry (MS)-based lipidomics and matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) was developed for the study of nephrotoxicity in mice exposed to 10 and 100 μg bisphenol S (BPS)/kg body weight/day. The BPS exposure remarkable perturbed abundances of 91 potential markers that mainly involved in five metabolic pathways...
February 12, 2018: Analytical Chemistry
Suzanne M de la Monte, Jared Kay, Emine B Yalcin, Jillian J Kril, Donna Sheedy, Greg T Sutherland
BACKGROUND: Chronic alcohol use disorders (AUD) are associated with white matter (WM) degeneration with altered myelin integrity. Matrix assisted laser desorption ionization-imaging mass spectrometry (MALDI-IMS) enables high throughput analysis of myelin lipid biochemical histopathology to help characterize disease mechanisms. PURPOSE: This study utilized MALDI-IMS to investigate frontal lobe WM myelin lipid abnormalities in AUD. METHODS: Standardized cores of formalin-fixed WM from Brodmann Area 4 (BA4) and BA8/9 of 20 postmortem AUD and 19 control adult human brains were embedded in carboxymethyl-cellulose, cryo-sectioned (8 μm), thaw-mounted onto indium tin oxide (ITO) -coated glass slides, and sublimed with 2,5-dihydroxybenzxoic acid (DHB) matrix...
August 17, 2017: Alcohol
Ignacy Rzagalinski, Nadine Hainz, Carola Meier, Thomas Tschernig, Dietrich A Volmer
Bile acids (BAs) play two vital roles in living organisms, as they are involved in (1) the secretion of cholesterol from liver, and (2) the lipid digestion/absorption in the intestine. Abnormal bile acid synthesis or secretion can lead to severe liver disorders. Even though there is extensive literature on the mass spectrometric determination of BAs in biofluids and tissue homogenates, there are no reports on the spatial distribution in the biliary network of the liver. Here, we demonstrate the application of high mass resolution/mass accuracy matrix-assisted laser desorption/ionization (MALDI)-Fourier-transform ion cyclotron resonance (FTICR) to MS imaging (MSI) of BAs at high spatial resolutions (pixel size, 25 μm)...
February 7, 2018: Journal of the American Society for Mass Spectrometry
Mandana Hunter, Nicholas J Demarais, Richard L M Faull, Angus C Grey, Maurice A Curtis
The subventricular zone is a key site of adult neurogenesis and is also implicated in neurodegenerative diseases and brain cancers. In the subventricular zone, cell proliferation, migration and differentiation of nascent stem cells and neuroblasts are regulated at least in part by lipids. The human subventricular zone is distinctly layered and each layer contains discrete cell types that support the processes of neuroblast migration and neurogenesis. We set out to determine the lipid signatures of each subventricular layer in the adult human brain (n = 4)...
February 7, 2018: Scientific Reports
Adrian Arendowski, Joanna Nizioł, Tomasz Ruman
A new methodology applicable for both - high resolution laser desorption/ionization mass spectrometry and mass spectrometry imaging of amino acids is presented. The MALDI-type target containing monoisotopic cationic 109 Ag nanoparticles (109 AgNPs) was used for rapid mass spectrometry measurements of eleven amino acids of different chemical properties. Amino acids were directly tested in 100000-fold concentration change conditions ranging from 100 μg/mL to 1 ng/mL which equates to 50 ng to 500 fg of amino acid per measurement spot...
February 7, 2018: Journal of Mass Spectrometry: JMS
Bingming Chen, Chuanzi OuYang, Zichuan Tian, Meng Xu, Lingjun Li
Introduced in 2000, atmospheric pressure (AP)/matrix-assisted laser desorption/ionization (MALDI) has attracted substantial attention in the mass spectrometry community due to its ease of sample introduction and handling, interchangeability with ESI source and capability of analyzing volatile species. In this study, an AP/MALDI source with ultra-high spatial resolution was coupled to a Q Exactive HF orbitrap mass spectrometer for high resolution in situ analysis by MALDI, laserspray ionization (LSI) and matrix assisted ionization (MAI) without instrument modification...
May 12, 2018: Analytica Chimica Acta
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