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MALDI Imaging

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https://www.readbyqxmd.com/read/29340649/optic-nerve-regeneration-after-crush-remodels-the-injury-site-molecular-insights-from-imaging-mass-spectrometry
#1
David T Stark, David M G Anderson, Jacky M K Kwong, Nathan Heath Patterson, Kevin L Schey, Richard M Caprioli, Joseph Caprioli
Purpose: Mammalian central nervous system axons fail to regenerate after injury. Contributing factors include limited intrinsic growth capacity and an inhibitory glial environment. Inflammation-induced optic nerve regeneration (IIR) is thought to boost retinal ganglion cell (RGC) intrinsic growth capacity through progrowth gene expression, but effects on the inhibitory glial environment of the optic nerve are unexplored. To investigate progrowth molecular changes associated with reactive gliosis during IIR, we developed an imaging mass spectrometry (IMS)-based approach that identifies discriminant molecular signals in and around optic nerve crush (ONC) sites...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29337283/variability-studies-of-allochthonous-stone-pine-pinus-pinea-l-plantations-in-chile-through-nut-protein-profiling
#2
V Loewe, R M Navarro-Cerrillo, R Sánchez Lucas, F J Ruiz Gómez, J Jorrín-Novo
Stone pine (Pinus pinea) is characterized by low differentiation of growth parameters, high phenotypic plasticity and low genetic variability; detecting its diversity in introduced Chilean populations is therefore relevant for conservation and breeding programs. Here, variability among allochthonous Stone pine populations in Chile was explored using electrophoresis-based proteomic analysis of pine nuts. Cones from 30 populations distributed along a climatic gradient in Chile were surveyed and sampled, and proteins were extracted from seed flour using the TCA-acetone precipitation protocol...
January 11, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29321555/fourier-transform-infrared-microscopy-enables-guidance-of-automated-mass-spectrometry-imaging-to-predefined-tissue-morphologies
#3
Jan-Hinrich Rabe, Denis A Sammour, Sandra Schulz, Bogdan Munteanu, Martina Ott, Katharina Ochs, Peter Hohenberger, Alexander Marx, Michael Platten, Christiane A Opitz, Daniel S Ory, Carsten Hopf
Multimodal imaging combines complementary platforms for spatially resolved tissue analysis that are poised for application in life science and personalized medicine. Unlike established clinical in vivo multimodality imaging, automated workflows for in-depth multimodal molecular ex vivo tissue analysis that combine the speed and ease of spectroscopic imaging with molecular details provided by mass spectrometry imaging (MSI) are lagging behind. Here, we present an integrated approach that utilizes non-destructive Fourier transform infrared (FTIR) microscopy and matrix assisted laser desorption/ionization (MALDI) MSI for analysing single-slide tissue specimen...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315936/biosynthesis-of-bioactive-diterpenoids-in-the-medicinal-plant-vitex-agnus-castus
#4
Allison Maree Heskes, Tamil C M Sundram, Berin Alain Boughton, Niels Bjerg Jensen, Nikolaj Lervad Hansen, Christoph Crocoll, Federico Cozzi, Simon Rasmussen, Britta Hamberger, Björn Hamberger, Dan Staerk, Birger Lindberg Møller, Irini Pateraki
Vitex agnus-castus L. (Lamiaceae) is a medicinal plant historically used throughout the Mediterranean region to treat menstrual cycle disorders and is still used today as a clinically effective treatment for premenstrual syndrome. The pharmaceutical activity of the plant extract is linked to its ability to lower prolactin levels. This feature has been attributed to the presence of dopaminergic diterpenoids that can bind to dopamine receptors in the pituitary gland. Phytochemical analyses of V. agnus-castus show that it contains an enormous array of structurally related diterpenoids and, as such holds potential as a rich source of new dopaminergic drugs...
January 8, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29313206/the-effect-of-collimating-lens-focusing-on-laser-beam-shape-in-matrix-assisted-laser-desorption-ionization-mass-spectrometry-maldi-ms
#5
Matthew B O'Rourke, Benjamin B A Raymond, Steven P Djordjevic, Matthew P Padula
Tissue imaging using matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a well-established technique that, in recent years, has seen wider adoption and novel application. Applications such imaging mass spectrometry (IMS) and biotyping are beginning to gain greater exposure and use; however, with limitations in optimization methods, producing the best result often relies on the ability to customize the physical characteristics of the instrumentation, a task that is challenging for most mass spectrometry laboratories...
January 8, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29310285/silver-nanoparticles-as-matrix-for-maldi-fticr-ms-profiling-and-imaging-of-diverse-lipids-in-brain
#6
Ming Guan, Zhen Zhang, Shilei Li, Jian'an Liu, Lu Liu, Hui Yang, Yangyang Zhang, Tie Wang, Zhenwen Zhao
Owing to the diversity of lipids, profiling and imaging multiple classes of lipids in one analysis by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is a great challenge. In this work, polyvinylpyrrolidone (PVP) capped silver nanoparticles (AgNPs) was used as the matrix for MALDI MSI for the first time to simultaneously analyze 10 classes of lipids from the brain. This analysis included fatty acids and their derivatives, sterols, CPAs, LPA and PAs, LPE and PEs, LPC and PCs, PS, Cers, SMs, and MAGs and DAGs, and other small metabolites...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29305797/anatomical-molecular-distribution-of-ephrina1-in-infarcted-mouse-heart-using-maldi-mass-spectrometry-imaging
#7
Stephan Lefcoski, Kimberly Kew, Shaun Reece, Maria J Torres, Justin Parks, Sky Reece, Lisandra E de Castro Brás, Jitka A I Virag
EphrinA1 is a tyrosine kinase receptor localized in the cellular membrane of healthy cardiomyocytes, the expression of which is lost upon myocardial infarction (MI). Intra-cardiac injection of the recombinant form of ephrinA1 (ephrinA1-Fc) at the time of ligation in mice has shown beneficial effects by reducing infarct size and myocardial necrosis post-MI. To date, immunohistochemistry and Western blotting comprise the only experimental approaches utilized to localize and quantify relative changes of ephrinA1 in sections and homogenates of whole left ventricle, respectively...
January 5, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29297598/visualization-of-the-distribution-of-anthocyanin-species-in-mice-eyeball-by-maldi-mass-spectrometry-imaging
#8
Shuhei Yamada, Nobuhiro Zaima, Yukihiro Yoshimura, Shiori Inaba, Takamichi Fujimori, Tetsuya Sogon, Tatsuya Moriyama
RATIONALE: Anthocyanins, which belong to a class of molecules called flavonoids, are known to have beneficial effects for both humans and animals. Many physiological functions have been attributed to anthocyanins since ancient times. The most important function is the relief of eyestrain, but the biodistribution of anthocyanins remains unknown. In this study, we analyzed the kinetics of anthocyanin species in mice eyeballs and surrounding tissues. METHODS: We used mice that were administered bilberry extract solution intraperitoneally...
January 3, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29295614/insights-into-the-maldi-process-after-matrix-deposition-by-sublimation-using-3d-tof-sims-imaging
#9
Sebastiaan Van Nuffel, Nicolas Elie, Ethan Yang, Julius Nouet, David Touboul, Pierre Chaurand, Alain Brunelle
Imaging mass spectrometry (IMS) has become a powerful tool to characterise the spatial distribution of biomolecules in thin tissue sections. In the case of matrix-assisted laser desorption ionisation (MALDI) IMS, homogeneous matrix deposition is critical to produce high quality ion images and sublimation in particular has shown to be an excellent matrix deposition method for the imaging of lipids. Matrix deposition by sublimation is, however, a completely solvent-free system, which ought to prevent the mixing of matrix and analytes thought to be necessary for successful MALDI...
January 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29289304/enhanced-coverage-of-lipid-analysis-and-imaging-by-matrix-assisted-laser-desorption-ionization-mass-spectrometry-via-a-strategy-with-an-optimized-mixture-of-matrices
#10
Jianing Wang, Chunyan Wang, Xianlin Han
In matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) analysis and imaging of lipids, comprehensive ionization of lipids simultaneously by a universal matrix is a very challenging problem. Ion suppression of readily ionizable lipids to others is common. To overcome this obstacle and enhance the coverage of MALDI MS analysis and imaging of lipids, we developed a novel strategy employing a mixture of matrices, each of which is capable of selective ionization of different lipid classes. Given that MALDI MS with either 9-aminoacridine (9-AA) or N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) yields weak in-source decay which is critical for analysis of complex biological samples and possesses orthogonal selectivity for ionization of lipid classes, we tested the mixtures of NEDC and 9-AA with different ratios for analysis of standard lipids and mouse brain lipid extracts...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29285820/maldi-imaging-detects-endogenous-digoxin-in-glioblastoma-cells-infected-by-zika-virus-would-it-be-the-oncolytic-key
#11
Estela de O Lima, Tatiane M Guerreiro, Carlos Fernando O R Melo, Diogo N de Oliveira, Daisy Machado, Marcelo Lancelloti, Rodrigo R Catharino
Recently, microcephaly cases have increased in Americas and have been matter of concern due to Zika virus (ZIKV) recent outbreak. Previous studies have shown that ZIKV-infected progenitor neuronal cells present morphological abnormalities and increased rates of cell death, which may be indicators of microcephaly causes. As recent studies indicate Zika virus' tropism for brain cells, how would a glioblastoma (GBM) lineage behave under ZIKV infection, considering GBM the most common and malignant brain tumor in adults, presenting extreme chemoresistance and high morbidity and mortality rates? The current trend of using genetically engineered oncolytic pathogens as a safe way to eliminate tumors is under development, with trials already in course...
December 28, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29275560/-research-progress-of-maldi-tof-ims-in-biomedicine-and-its-application-prospect-in-forensic-sciences
#12
REVIEW
G H Ren, R H Weng, Y Shi, P Huang, Z D Li, Y Shao, K F Deng, N G Liu, Y J Chen
Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS) can analysis unknown compounds in sections and obtain molecule imaging by scanning biological tissue sections, which has become a powerful tool for the research of biomarker, lipid distribution and drug metabolism, etc. This article reviews the application of this technique in protein identification, clinical application, drug discovery, lipid research and brain injury.
October 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29247380/utilizing-mass-spectrometry-imaging-to-map-the-thyroid-hormones-triiodothyronine-and-thyroxine-in-xenopus-tropicalis-tadpoles
#13
Naoko Goto-Inoue, Tomohiko Sato, Mizuki Morisasa, Akihiko Kashiwagi, Keiko Kashiwagi, Yuki Sugiura, Eiji Sugiyama, Makoto Suematsu, Tsukasa Mori
Thyroid hormones are not only responsible for thermogenesis and energy metabolism in animals, but also have an important role in cell differentiation and development. Amphibian metamorphosis provides an excellent model for studying the remodeling of the body. This metamorphic organ remodeling is induced by thyroid hormones, and a larval body is thus converted into an adult one. The matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) imaging technology is expected to be a suitable tool for investigating small bioreactive molecules...
December 15, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29242606/murine-stroma-adopts-a-human-like-metabolic-phenotype-in-the-pdx-model-of-colorectal-cancer-and-liver-metastases
#14
Arnaud Blomme, Gaetan Van Simaeys, Gilles Doumont, Brunella Costanza, Justine Bellier, Yukihiro Otaka, Félicie Sherer, Pierre Lovinfosse, Sébastien Boutry, Ana Perez Palacios, Edwin De Pauw, Touko Hirano, Takehiko Yokobori, Roland Hustinx, Akeila Bellahcène, Philippe Delvenne, Olivier Detry, Serge Goldman, Masahiko Nishiyama, Vincent Castronovo, Andrei Turtoi
Cancer research is increasingly dependent of patient-derived xenograft model (PDX). However, a major point of concern regarding the PDX model remains the replacement of the human stroma with murine counterpart. In the present work we aimed at clarifying the significance of the human-to-murine stromal replacement for the fidelity of colorectal cancer (CRC) and liver metastasis (CRC-LM) PDX model. We have conducted a comparative metabolic analysis between 6 patient tumors and corresponding PDX across 4 generations...
December 15, 2017: Oncogene
https://www.readbyqxmd.com/read/29234137/ratiometric-mass-spectrometry-for-cell-identification-and-quantitation-using-intracellular-dual-biomarkers
#15
Xiaoming Chen, Fangjie Wo, Jiang Chen, Jie Tan, Tao Wang, Xiao Liang, Jianmin Wu
This study proposed an easy-to-use method for cell identification and quantitation by ratiometric matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Two pairs of MS peaks in the molecular fingerprint of cells were selected as intracellular dual-biomarkers due to the stability and specificity of their ratio values in different types of hepatocellular cancer (HCC) cell lines. Five types of HCC cells can be thereafter differentiated based on these two pairs of intracellular peptides/proteins...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232991/mass-spectrometry-imaging-of-illicit-drugs-in-latent-fingerprints-by-matrix-free-and-matrix-assisted-desorption-ionization-techniques
#16
Anton Skriba, Vladimir Havlicek
Compared with classical matrix-assisted laser-desorption ionization mass spectrometry (MALDI), the matrix free-based strategies generate a cleaner background, without significant noise or interference coming from an applied matrix, which is beneficial for the analysis of small molecules, such as drugs of abuse. In this work, we probed the detection efficiency of methamphetamine, heroin and cocaine in nanostructure-assisted laser desorption-ionization (NALDI) and desorption electrospray ionization and compared the sensitivity of these two matrix-free tools with a standard MALDI mass spectrometry experiment...
January 1, 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29229934/bis-monoacylglycero-phosphate-lipids-in-the-retinal-pigment-epithelium-implicate-lysosomal-endosomal-dysfunction-in-a-model-of-stargardt-disease-and-human-retinas
#17
David M G Anderson, Zsolt Ablonczy, Yiannis Koutalos, Anne M Hanneken, Jeffrey M Spraggins, M Wade Calcutt, Rosalie K Crouch, Richard M Caprioli, Kevin L Schey
Stargardt disease is a juvenile onset retinal degeneration, associated with elevated levels of lipofuscin and its bis-retinoid components, such as N-retinylidene-N-retinylethanolamine (A2E). However, the pathogenesis of Stargardt is still poorly understood and targeted treatments are not available. Utilizing high spatial and high mass resolution matrix assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS), we determined alterations of lipid profiles specifically localized to the retinal pigment epithelium (RPE) in Abca4 -/- Stargardt model mice compared to their relevant background strain...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29227110/itraq-quantitative-proteomic-profiling-and-maldi-msi-of-colon-cancer-spheroids-treated-with-combination-chemotherapies-in-a-3d-printed-fluidic-device
#18
Gabriel J LaBonia, Katelyn R Ludwig, C Bruce Mousseau, Amanda B Hummon
For a patient with metastatic colorectal cancer there are limited clinical options aside from chemotherapy. Unfortunately, the development of new chemotherapeutics is a long and costly process. New methods are needed to identify promising drug candidates earlier in the drug development process. Most chemotherapies are administered to patients in combinations. Here, an in vitro platform is used to assess the penetration and metabolism of combination chemotherapies in three-dimensional colon cancer cell cultures, or spheroids...
December 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29226683/permeability-barriers-for-nicotine-and-mannitol-in-porcine-buccal-mucosa-studied-by-high-resolution-maldi-mass-spectrometry-imaging
#19
Eva Marxen, Jette Jacobsen, Birgitte Hyrup, Christian Janfeldt
Improved nicotine permeability across buccal mucosa may enable more effective oromucosal nicotine replacement therapy products. It is essential to know the location and composition of the main barrier for drug diffusion to enhance the drug permeability. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is a rapidly evolving technique that can be used to image the spatial distribution of drugs and drug metabolites in tissue cryo-sections, without prior labeling of the drug. In this study, the distribution of nicotine and mannitol in porcine buccal mucosa was imaged with 10 µm spatial resolution after apical as well as submucosal application of the drugs, in order to localize the main permeability barrier(s)...
December 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29226434/protein-identification-in-imaging-mass-spectrometry-through-spatially-targeted-liquid-micro-extractions
#20
Daniel J Ryan, David Nei, Boone M Prentice, Kristie L Rose, Richard M Caprioli, Jeffrey M Spraggins
RATIONALE: Liquid extraction surface analysis (LESA) can be used to generate spatially-directed protein identifications in an imaging mass spectrometry (IMS) workflow. This approach involves the use of robotic micro-extractions coupled to online liquid chromatography (LC). We have characterized the extraction efficiency of this method as well as its ability to identify proteins from a matrix assisted laser/desorption ionization (MALDI) IMS experiment. METHODS: Proteins and peptides were extracted from transverse sections of a rat brain and sagittal sections of a mouse pup using liquid surface extractions...
December 10, 2017: Rapid Communications in Mass Spectrometry: RCM
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