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MALDI Imaging

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https://www.readbyqxmd.com/read/28336905/a-nanostructured-matrices-assessment-to-study-drug-distribution-in-solid-tumor-tissues-by-mass-spectrometry-imaging
#1
Silvia Giordano, Valentina Pifferi, Lavinia Morosi, Melinda Morelli, Luigi Falciola, Giuseppe Cappelletti, Sonja Visentin, Simonetta A Licandro, Roberta Frapolli, Massimo Zucchetti, Roberta Pastorelli, Laura Brunelli, Maurizio D'Incalci, Enrico Davoli
The imaging of drugs inside tissues is pivotal in oncology to assess whether a drug reaches all cells in an adequate enough concentration to eradicate the tumor. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI) is one of the most promising imaging techniques that enables the simultaneous visualization of multiple compounds inside tissues. The choice of a suitable matrix constitutes a critical aspect during the development of a MALDI-MSI protocol since the matrix ionization efficiency changes depending on the analyte structure and its physico-chemical properties...
March 21, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28335978/systematic-assessment-of-surfactants-for-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
#2
Bijay Banstola, Eulalie T Grodner, Fan Cao, Fabrizio Donnarumma, Kermit K Murray
A systematic method for evaluation of MALDI profiling and imaging was developed and applied to the use of three surfactants, sodium dodecyl sulfate (SDS), Triton X-100, and Tween 20, on rat brain tissue. For profiling studies, mass spectra were acquired from regular arrays of spots with manually deposited surfactant and matrix. The studies recorded the total number of peaks in the mass spectra from 2 to 20 kDa and compared the number of peaks and peak intensities with and without surfactant. It was found that SDS decreases the total number of peaks at all concentrations but does lead to an increase in the number of peaks below 5 kDa...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28329870/application-of-imaging-techniques-to-cases-of-drug-induced-crystal-nephropathy-in-preclinical-studies
#3
B Lenz, A Brink, M Siam, A De Paepe, S Bassett, A Eichinger-Chapelon, P Maliver, R Neff, U Niederhauser, B Steinhuber, R Zurbach, T Singer, C Funk, F Schuler, M Albassam, S Schadt
A number of drugs can cause precipitates within renal tubules leading to crystal nephropathy. Crystal nephropathy is usually an exposure-related finding and is not uncommon in preclinical studies, where high doses are tested. An understanding of the nature of precipitates is important for human risk assessment and further development. Our aim was to investigate the ability of various imaging techniques to detect the presence of drugs or metabolites in renal crystals. We applied matrix-assisted laser desorption/ionization - Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS) imaging, Raman and infrared microspectroscopy, scanning electron microscopy coupled with energy dispersive X-ray (SEM/EDX) spectroscopy and standard histopathology to cases of drug-induced crystal nephropathy, induced in rodents and primates by four compounds...
February 27, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28325940/one-pot-synthesis-of-four-coordinate-boron-iii-complexes-by-the-ligand-promoted-organic-group-migration-between-boronic-acids
#4
Venkata S Sadu, Hye-Rin Bin, Do-Min Lee, Kee-In Lee
Multidisciplinary applications of four-coordinate boron(III) complexes make them very attractive and challenging research field in chemistry, biology and material sciences. The dual role played by boron atom in stabilising the chelate ligand and enhancing the π-conjugation makes them very useful as luminescent materials for organic electronics and photonics, and sensing and imaging probes for biomedical purposes. The conventional methods involve the use of diarylborinic acids or anhydrides and triaryl boranes, which are made from organometallic reagents...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28318232/histology-compatible-maldi-mass-spectrometry-based-imaging-of-neuronal-lipids-for-subsequent-immunofluorescent-staining
#5
Ibrahim Kaya, Wojciech Michno, Dimitri Brinet, Yasmine Iacone, Giulia Zanni, Kaj Blennow, Henrik Zetterberg, Jörg Hanrieder
Matrix assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) enables acquisition of spatial distribution maps for molecular species in situ. This can provide comprehensive insights on the pathophysiology of different diseases. However, current sample preparation and MALDI-IMS acquisition methods have limitations in preserving molecular and histological tissue morphology, resulting in interfered correspondence of MALDI-IMS data with subsequently acquired immunofluorescent staining results...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28306235/gcib-tof-sims-high-resolution-imaging-of-cardiolipin-speciation-in-the-brain-identification-of-molecular-losses-after-traumatic-injury
#6
Hua Tian, Louis J Sparvero, Andrew A Amoscato, Anna Bloom, Hulya Bayir, Valerian E Kagan, Nicholas Winograd
Gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) has shown the full potential of mapping intact lipids in biological systems with better than 10 µm lateral resolution. This study investigated further the capability of GCIB-SIMS in imaging high-mass signals from intact cardiolipin (CL) and gangliosides in normal brain and the effect of a controlled cortical impact model (CCI) of traumatic brain injury (TBI) on their distribution. A combination of enzymatic and chemical treatments was employed to suppress the signals from the most abundant phospholipids (phosphatidylcholine (PC) and phosphatidylethanolamine (PE)) and enhance the signals from the low abundance CLs and gangliosides to allow their GCIB-SIMS detection at 8 and 16 µm spatial resolution...
March 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28300637/integrated-mass-spectrometry-imaging-and-omics-workflows-on-the-same-tissue-section-using-grid-aided-parafilm-assisted-microdissection
#7
Jusal Quanico, Julien Franck, Maxence Wisztorski, Michel Salzet, Isabelle Fournier
BACKGROUND: In spite of the number of applications describing the use of MALDI MSI, one of its major drawbacks is the limited capability of identifying multiple compound classes directly on the same tissue section. METHODS: We demonstrate the use of grid-aided, parafilm-assisted microdissection to perform MALDI MS imaging and shotgun proteomics and metabolomics in a combined workflow and using only a single tissue section. The grid is generated by microspotting acid dye 25 using a piezoelectric microspotter, and this grid was used as a guide to locate regions of interest and as an aid during manual microdissection...
March 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28294158/improved-maldi-imaging-ms-analysis-of-phospholipids-using-graphene-oxide-as-new-matrix
#8
Zhongjie Wang, Yan Cai, Yi Wang, Xinwen Zhou, Ying Zhang, Haojie Lu
Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is an increasingly important technique for detection and spatial localization of phospholipids on tissue. Due to the high abundance and being easy-to-ionize of phosphatidylcholine (PC), therefore, selecting matrix to yield signals of other lipids has become the most crucial factor for a successful MALDI-IMS analysis of phospholipids. Herein, graphene oxide (GO) was proposed as a new matrix to selectively enhance the detection of other types of phospholipids that are frequently suppressed by the presence of PC in positive mode...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28290124/the-characterization-of-laser-ablation-patterns-and-a-new-definition-of-resolution-in-matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-maldi-ims
#9
Matthew B O'Rourke, Benjamin B A Raymond, Matthew P Padula
Matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) is a technique that has seen a sharp rise in both use and development. Despite this rapid adoption, there have been few thorough investigations into the actual physical mechanisms that underlie the acquisition of IMS images. We therefore set out to characterize the effect of IMS laser ablation patterns on the surface of a sample. We also concluded that the governing factors that control spatial resolution have not been correctly defined and therefore propose a new definition of resolution...
March 13, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28289929/co-encapsulation-of-amyloglucosidase-with-starch-and-saccharomyces-cerevisiae-as-basis-for-a-long-lasting-co2-release
#10
Pascal Humbert, Marina Vemmer, Marco Giampà, Hanna Bednarz, Karsten Niehaus, Anant V Patel
CO2 is known as a major attractant for many arthropod pests which can be exploited for pest control within novel attract-and-kill strategies. This study reports on the development of a slow-release system for CO2 based on calcium alginate beads containing granular corn starch, amyloglucosidase and Saccharomyces cerevisiae. Our aim was to evaluate the conditions which influence the CO2 release and to clarify the biochemical reactions taking place within the beads. The amyloglucosidase was immobilized with a high encapsulation efficiency of 87% in Ca-alginate beads supplemented with corn starch and S...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28280112/lipidomic-characterization-and-localization-of-phospholipids-in-the-human-lung
#11
Karin A Zemski Berry, Robert C Murphy, Beata Kosmider, Robert J Mason
Lipids play a central role in lung physiology and pathology, however a comprehensive lipidomic characterization of human pulmonary cells relevant to disease has not been performed. The cells involved in lung host defense, including alveolar macrophages (AM), bronchial epithelial cells (BEC), and alveolar type II cells (ATII), were isolated from human subjects and lipidomic analysis by LC-MS and LC-MS/MS was performed. Additionally, pieces of lung tissue from the same donors were analyzed by MALDI imaging mass spectrometry (IMS) in order to determine lipid localization in the tissue...
March 9, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28276159/comparative-proteome-analysis-of-wild-type-and-klotho-knockout-mouse-kidneys-using-a-combination-of-maldi-ims-and-lc-ms-ms
#12
Yoko Fujino, Tomoko Minamizaki, Ikue Hayashi, Asako Kawakami, Takaaki Miyaji, Kaoru Sakurai, Hirotaka Yoshioka, Katsuyuki Kozai, Mitsugi Okada, Yuji Yoshiko
PURPOSE: Mutation of the klotho gene in mice elicits a syndrome resembling accelerated human aging. However, there is limited evidence for the role of Klotho in the kidney. We conducted a comparative proteome analysis of wild-type (WT) and Klotho-knockout (kl(-/-) ) mouse kidneys to identify proteins involved in Klotho deficiency. EXPERIMENTAL DESIGN: MALDI-IMS of frozen kidney sections from 7-week-old male WT and kl(-/-) mice was used to determine genotype-specific differences in the MS distribution...
March 8, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28271569/n-glycan-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging-protocol-for-formalin-fixed-paraffin-embedded-tissues
#13
Matthew T Briggs, Yin Ying Ho, Gurjeet Kaur, Martin K Oehler, Arun V Everest-Dass, Nicolle H Packer, Peter Hoffmann
RATIONALE: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) of the proteome of a tissue has been an established technique for the past decade. In the last few years, MALDI-MSI of the N-glycome has emerged as a novel MALDI-MSI technique. To assess the accuracy and clinical significance of the N-linked glycan spatial distribution, we have developed a method that utilises MALDI-MSI followed by liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) in order to assign glycan structures to the differentiating MALDI-MSI glycan masses released from the tissue glycoproteins...
March 8, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28263004/improving-the-analyte-ion-signal-in-matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-via-electrospray-deposition-by-enhancing-incorporation-of-the-analyte-in-the-matrix
#14
Brian J Malys, Kevin G Owens
RATIONAL: Matrix assisted laser desorption ionization (MALDI) is widely used as the ionization method in high resolution chemical imaging studies that seek to visualize the distribution of analytes within sectioned biological tissues. This work extends the use of electrospray deposition (ESD) to apply matrix with an additional solvent spray to incorporate and homogenize analyte within the matrix overlayer. METHODS: Analytes and matrix are sequentially and independently applied by ESD to create a sample from which spectra are collected, mimicking a MALDI imaging mass spectrometry (IMS) experiment...
March 6, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28259683/inhibition-of-sebum-production-with-the-acetyl-coenzyme-a-carboxylase-inhibitor-olumacostat-glasaretil
#15
David W Hunt, Geoffrey C Winters, Roger W Brownsey, Jerzy E Kulpa, Kathryn L Gilliland, Diane M Thiboutot, Hans E Hofland
Olumacostat glasaretil (OG) is a small molecule inhibitor of acetyl coenzyme A carboxylase (ACC), the enzyme which controls the first, rate limiting step in fatty acid biosynthesis. Inhibition of ACC activity in the sebaceous glands is designed to substantially impact sebum production since over 80% of human sebum components contain fatty acids. OG inhibits de novo lipid synthesis in primary and transformed human sebocytes. TrueMass® Sebum Panel analyses revealed a reduction in saturated and monounsaturated fatty acyl chains across lipid species, including di- and triacylglycerols, phospholipids, cholesteryl esters, and wax esters in OG treated sebocytes...
March 1, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28251574/dapne-with-micro-capillary-separatory-chemistry-coupled-to-maldi-ms-for-the-analysis-of-polar-and-non-polar-lipid-metabolism-in-one-cell
#16
Jason S Hamilton, Roberto Aguilar, Robby A Petros, Guido F Verbeck
The cellular metabolome is considered to be a representation of cellular phenotype and cellular response to changes to internal or external events. Methods to expand the coverage of the expansive physiochemical properties that makeup the metabolome currently utilize multi-step extractions and chromatographic separations prior to chemical detection, leading to lengthy analysis times. In this study, a single-step procedure for the extraction and separation of a sample using a micro-capillary as a separatory funnel to achieve analyte partitioning within an organic/aqueous immiscible solvent system is described...
March 1, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28248242/a-proof-of-concept-to-bridge-the-gap-between-mass-spectrometry-imaging-protein-identification-and-relative-quantitation-msi-lc-ms-ms-lf
#17
Laëtitia Théron, Delphine Centeno, Cécile Coudy-Gandilhon, Estelle Pujos-Guillot, Thierry Astruc, Didier Rémond, Jean-Claude Barthelemy, Frédéric Roche, Léonard Feasson, Michel Hébraud, Daniel Béchet, Christophe Chambon
Mass spectrometry imaging (MSI) is a powerful tool to visualize the spatial distribution of molecules on a tissue section. The main limitation of MALDI-MSI of proteins is the lack of direct identification. Therefore, this study focuses on a MSI~LC-MS/MS-LF workflow to link the results from MALDI-MSI with potential peak identification and label-free quantitation, using only one tissue section. At first, we studied the impact of matrix deposition and laser ablation on protein extraction from the tissue section...
October 26, 2016: Proteomes
https://www.readbyqxmd.com/read/28239976/high-speed-maldi-ms-ms-imaging-mass-spectrometry-using-continuous-raster-sampling
#18
Boone M Prentice, Chad W Chumbley, Richard M Caprioli
No abstract text is available yet for this article.
August 2016: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28228396/case-of-a-cerebral-abscess-caused-by-porphyromonas-gingivalis-in-a-subject-with-periodontitis
#19
Frederic Van der Cruyssen, Koenraad Grisar, Honorine Maes, Constantinus Politis
We report the case of a 65-year-old man presenting with generalised seizures after developing a right frontal brain abscess. Stereotactic aspiration and subsequent matrix assisted laser desorption/ionisation time-of-flight analyzer (MALDI-TOF) spectrometry revealed Porphyromonas gingivalis as the only causative anaerobe microorganism. Secondary incision and drainage was required due to neurological deterioration with increased dimensions of the abscess, intracranial pressure and formation of a subdural occipitoparietal empyema...
February 22, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28225596/top-down-metabolomic-approaches-for-nitrogen-containing-metabolites
#20
Ryo Nakabayashi, Kei Hashimoto, Kiminori Toyooka, Kazuki Saito
Streamlining the processes that reveal heteroatom-containing metabolites and their biosynthetic genes is essential in integrated metabolomics studies. These metabolites are especially targeted for their potential pharmaceutical activities. By using a Fourier-transform ion cyclotron resonance-mass spectrometry (FTICR-MS) instrument, we provide top-down targeted metabolomic analyses using ultrahigh-resolution liquid chromatography-mass spectrometry (LC-MS), high-resolution matrix-assisted laser desorption/ionization (MALDI), and high-resolution imaging mass spectrometry (IMS) with (15)N labeling of nitrogen-containing metabolites...
February 22, 2017: Analytical Chemistry
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