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MALDI Imaging

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https://www.readbyqxmd.com/read/28091541/apelin-receptor-homodimer-oligomers-revealed-by-single-molecule-imaging-and-novel-g-protein-dependent-signaling
#1
Xin Cai, Bo Bai, Rumin Zhang, Chunmei Wang, Jing Chen
The apelin receptor (APJ) belongs to family A of the G protein-coupled receptors (GPCRs) and is a potential pharmacotherapeutic target for heart failure, hypertension, and other cardiovascular diseases. There is evidence APJ heterodimerizes with other GPCRs; however, the existence of APJ homodimers and oligomers remains to be investigated. Here, we measured APJ monomer-homodimer-oligomer interconversion by monitoring APJ dynamically on cells and compared their proportions, spatial arrangement, and mobility using total internal reflection fluorescence microscopy, resonance energy transfer, and proximity biotinylation...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28087424/clinical-applications-of-maldi-imaging-technologies-in-cancer-and-neurodegenerative-diseases
#2
REVIEW
Yasemin Ucal, Zeynep Aslıhan Durer, Hakan Atak, Elif Kadioglu, Betul Sahin, Abdurrahman Coskun, Ahmet Tarık Baykal, Aysel Ozpinar
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) imaging mass spectrometry (IMS) enables localization of analytes of interest along with histology. More specifically, MALDI-IMS identifies the distributions of proteins, peptides, small molecules, lipids, and drugs and their metabolites in tissues, with high spatial resolution. This unique capacity to directly analyze tissue samples without the need for lengthy sample preparation reduces technical variability and renders MALDI-IMS ideal for the identification of potential diagnostic and prognostic biomarkers and disease gradation...
January 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28087213/cholesterolomics-an-update
#3
William J Griffiths, Jonas Abdel-Khalik, Eylan Yutuc, Alwena H Morgan, Ian Gilmore, Thomas Hearn, Yuqin Wang
Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome...
January 10, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28068764/topochemical-azide-alkyne-cycloaddition-reaction-in-gels-size-tunable-synthesis-of-triazole-linked-polypeptides
#4
Baiju P Krishnan, Kana M Sureshan
Though topochemical reactions are attractive, the difficulty associated with crystallization such as low yield, unsuitability for large scale synthesis etc warranted the exploitation of other self-assembled media for topochemical reactions. We synthesized a dipeptide gelator decorated with azide and alkyne at its termini, N3-Ala-Val-NHCH2-C≡CH, which is designed to self-assemble through intermolecular hydrogen bonds to β-sheets thereby placing the azide and alkyne motifs at proximity. As anticipated, this peptide forms gels in organic solvents and water via hydrogen-bonded β-sheet assembly as evidenced from IR spectroscopy and PXRD profiling...
January 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28050871/five-micron-high-resolution-maldi-mass-spectrometry-imaging-with-simple-interchangeable-multi-resolution-optical-system
#5
Adam D Feenstra, Maria Emilia Dueñas, Young Jin Lee
High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. In this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from ~9 μm practical laser spot size to a practical laser spot size of ~4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length...
January 3, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28042343/limited-tumor-tissue-drug-penetration-contributes-to-primary-resistance-against-angiogenesis-inhibitors
#6
Szilvia Torok, Melinda Rezeli, Olga Kelemen, Akos Vegvari, Kenichi Watanabe, Yutaka Sugihara, Anna Tisza, Timea Marton, Ildiko Kovacs, Jozsef Tovari, Viktoria Laszlo, Thomas H Helbich, Balazs Hegedus, Thomas Klikovits, Mir Alireza Hoda, Walter Klepetko, Sandor Paku, Gyorgy Marko-Varga, Balazs Dome
Resistance mechanisms against antiangiogenic drugs are unclear. Here, we correlated the antitumor and antivascular properties of five different antiangiogenic receptor tyrosine kinase inhibitors (RTKIs) (motesanib, pazopanib, sorafenib, sunitinib, vatalanib) with their intratumoral distribution data obtained by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). In the first mouse model, only sunitinib exhibited broad-spectrum antivascular and antitumor activities by simultaneously suppressing vascular endothelial growth factor receptor-2 (VEGFR2) and desmin expression, and by increasing intratumoral hypoxia and inhibiting both tumor growth and vascularisation significantly...
2017: Theranostics
https://www.readbyqxmd.com/read/28028548/spatial-molecular-architecture-of-the-microbial-community-of-a-peltigera-lichen
#7
Neha Garg, Yi Zeng, Anna Edlund, Alexey V Melnik, Laura M Sanchez, Hosein Mohimani, Alexey Gurevich, Vivian Miao, Stefan Schiffler, Yan Wei Lim, Tal Luzzatto-Knaan, Shengxin Cai, Forest Rohwer, Pavel A Pevzner, Robert H Cichewicz, Theodore Alexandrov, Pieter C Dorrestein
Microbes are commonly studied as individual species, but they exist as mixed assemblages in nature. At present, we know very little about the spatial organization of the molecules, including natural products that are produced within these microbial networks. Lichens represent a particularly specialized type of symbiotic microbial assemblage in which the component microorganisms exist together. These composite microbial assemblages are typically comprised of several types of microorganisms representing phylogenetically diverse life forms, including fungi, photosymbionts, bacteria, and other microbes...
November 2016: MSystems
https://www.readbyqxmd.com/read/28017863/metabolomic-profiling-of-prostate-cancer-by-matrix-assisted-laser-desorption-ionization-fourier-transform-ion-cyclotron-resonance-mass-spectrometry-imaging-using-matrix-coating-assisted-by-an-electric-field-mcaef
#8
Xiaodong Wang, Jun Han, Darryl B Hardie, Juncong Yang, Jingxi Pan, Christoph H Borchers
In this work, we combined the use of two MALDI matrices (quercetin and 9-aminoacridine), a recently developed new matrix coating technique - matrix coating assisted by an electric field (MCAEF), and matrix-assisted laser desorption/ionization - Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICRMS) to detect and image endogenous compounds in the cancerous and non-cancerous regions of three human prostate cancer (stage II) tissue specimens. After three rounds of imaging data acquisitions (i...
December 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28012871/quantification-of-low-molecular-weight-compounds-by-maldi-imaging-mass-spectrometry-a-tutorial-review
#9
REVIEW
Ignacy Rzagalinski, Dietrich A Volmer
Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) permits label-free in situ analysis of chemical compounds directly from the surface of two-dimensional biological tissue slices. It links qualitative molecular information of compounds to their spatial coordinates and distribution within the investigated tissue. MALDI-MSI can also provide the quantitative amounts of target compounds in the tissue, if proper calibration techniques are performed. Obviously, as the target molecules are embedded within the biological tissue environment and analysis must be performed at their precise locations, there is no possibility for extensive sample clean-up routines or chromatographic separations as usually performed with homogenized biological materials; ion suppression phenomena therefore become a critical side effect of MALDI-MSI...
December 22, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28010844/pharmacokinetic-study-based-on-a-matrix-assisted-laser-desorption-ionization-quadrupole-ion-trap-time-of-flight-imaging-mass-microscope-combined-with-a-novel-relative-exposure-approach-a-case-of-octreotide-in-mouse-target-tissues
#10
Tai Rao, Yuhao Shao, Naoki Hamada, Yanmin Li, Hui Ye, Dian Kang, Boyu Shen, Xinuo Li, Xiaoxi Yin, Zhangpei Zhu, Haofeng Li, Lin Xie, Guangji Wang, Yan Liang
Application of imaging mass spectrometry in drug pharmacokinetics remains challenging due to its weak quantitative capability. Herein, an imaging mass microscope (iMScope), equipped with an optical microscope, an atmospheric pressure ion-source chamber for matrix-assisted laser desorption/ionization (AP-MALDI) and a hybrid quadrupole ion trap time-of-flight (QIT-TOF) analyzer, was first validated and applied to visualize drug disposition in vivo. The distribution and elimination rate of the therapeutic peptide octreotide, a long-acting analogue of the natural hormone somatostatin, was first calculated based on the data determined by iMScope system combining a novel relative exposure strategy...
February 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28006919/bacterial-analysis-by-laser-desorption-ionization-mass-spectrometry-on-amorphous-silicon
#11
Shin Hye Kim, Jeongkwon Kim, Seung-Hyun Jo, Jeong-Hoon Kim, Kyung Joong Kim, Sohee Yoon
Lipid profiling in nine bacterial species has been accomplished by laser desorption ionization mass spectrometry (LDI-MS) using amorphous silicon (a-Si) thin film with 100 nm thickness. Lipid ions could be generated by LDI on a-Si regardless of ion acquisition modes because of a thermal property of a-Si to govern laser-induced surface heating. In a comparative study of lipid profiling in Bacillus lichemiformis by LDI-MS and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), LDI-MS on a-Si shows a higher efficiency in lipid and lipopeptide detection than MALDI-MS...
December 22, 2016: Biointerphases
https://www.readbyqxmd.com/read/28000726/heterogeneity-of-paclitaxel-distribution-in-different-tumor-models-assessed-by-maldi-mass-spectrometry-imaging
#12
Silvia Giordano, Massimo Zucchetti, Alessandra Decio, Marta Cesca, Ilaria Fuso Nerini, Marika Maiezza, Mariella Ferrari, Simonetta Andrea Licandro, Roberta Frapolli, Raffaella Giavazzi, D'Incalci Maurizio, Enrico Davoli, Lavinia Morosi
The penetration of anticancer drugs in solid tumors is important to ensure the therapeutic effect, so methods are needed to understand drug distribution in different parts of the tumor. Mass spectrometry imaging (MSI) has great potential in this field to visualize drug distribution in organs and tumor tissues with good spatial resolution and superior specificity. We present an accurate and reproducible imaging method to investigate the variation of drug distribution in different parts of solid tumors. The method was applied to study the distribution of paclitaxel in three ovarian cancer models with different histopathological characteristics and in colon cancer (HCT116), breast cancer (MDA-MB-231) and malignant pleural mesothelioma (MPM487)...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27989120/tracking-invisible-transformations-of-physisorbed-monolayers-ldi-tof-and-maldi-tof-mass-spectrometry-as-complements-to-stm-imaging
#13
Jian He, Chen Fang, Russell A Shelp, Matthew B Zimmt
Triphenyleneethynylene (TPEE) derivatives bearing one long aliphatic chain on each terminal aryl ring and two short aliphatic chains on the central aryl ring (core chains) self-assemble single component and 1-D patterned, two-component, crystalline monolayers at the solution-graphite interface. The monolayer morphology directs the core chains off the graphite, making them accessible for chemical reactions but invisible to imaging by scanning tunneling microscopy (STM). This precludes using STM to monitor transformations of the core chains, either by reaction or solution-monolayer exchange of TPEE molecules...
December 30, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27989075/maldi-imaging-mass-spectrometry-as-a-lipidomic-approach-to-heart-valve-research
#14
Peggi M Angel, Ahmed S Bayoumi, Robert B Hinton, Yan Ru Su, David Bichell, John E Mayer, H Scott Baldwin, Richard M Caprioli
BACKGROUND: Valvular disease is characterized in part by lipid deposition, but systematic analysis of the patterns of global lipid expression in healthy and diseased valve tissues are unknown. This is due in part to tissue limitations for lipidomic preparations and technologies for evaluating lipid distribution in tissues. The study aim was to examine the application of matrixassisted laser desorption ionization imaging mass spectrometry (MALDI IMS) to the aortic valve during development and disease, as an approach to detect and map lipids and ultimately better understand valve structure and function...
March 2016: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/27987025/spri-maldi-ms-characterization-and-identification-of-a-kinase-from-cell-lysate-by-specific-interaction-with-different-designed-ankyrin-repeat-proteins
#15
Ulrike Anders, Jonas V Schaefer, Fatima-Ezzahra Hibti, Chiraz Frydman, Detlev Suckau, Andreas Plückthun, Renato Zenobi
We report on the direct coupling of surface plasmon resonance imaging (SPRi) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for the investigation of specific, non-covalent interactions, using the example of designed ankyrin repeat proteins (DARPins) and ribosomal protein S6 kinase 2 (RPS6KA2) directly from lysate of SH-SY5Y cells, derived from human bone marrow. Due to an array format, tracing of binding kinetics of numerous DARPins simultaneously and in real time becomes possible...
December 16, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27984697/delineating-amyloid-plaque-associated-neuronal-sphingolipids-in-transgenic-alzheimer-s-disease-mice-tgarcswe-using-maldi-imaging-mass-spectrometry
#16
Ibrahim Kaya, Dimitri Brinet, Wojciech Michno, Stina Syvänen, Dag Sehlin, Henrik Zetterberg, Kaj Blennow, Jörg Hanrieder
The major pathological hallmarks of Alzheimer's disease (AD) are the progressive aggregation and accumulation of beta-amyloid (Aβ) and hyperphosphorylated tau protein into neurotoxic deposits. Aβ aggregation has been suggested as the critical early inducer, driving the disease progression. However, the factors that promote neurotoxic Aβ aggregation remain elusive. Imaging mass spectrometry (IMS) is a powerful technique to comprehensively elucidate the spatial distribution patterns of lipids, peptides, and proteins in biological tissue sections...
January 10, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/27975444/balance-of-radicalar-cationazed-and-protonated-ions-of-nonpolar-compounds-in-maldi-mass-spectrometry-perspectives-for-volatile-secondary-metabolites-dynamic-analysis-and-imaging-generation
#17
T Guaratini, J N Mendonça, Icc Turatti, Jlc Lopes, N P Lopes
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27956354/maldi-imaging-delineates-hippocampal-glycosphingolipid-changes-associated-with-neurotoxin-induced-proteopathy-following-neonatal-bmaa-exposure
#18
Oskar Karlsson, Wojciech Michno, Yusuf Ransome, Jörg Hanrieder
The environmental toxin β-N-methylamino-L-alanine (BMAA) has been proposed to contribute to neurodegenerative diseases. We have previously shown that neonatal exposure to BMAA results in dose-dependent cognitive impairments, proteomic alterations and progressive neurodegeneration in the hippocampus of adult rats. A high BMAA dose (460mg/kg) also induced intracellular fibril formation, increased protein ubiquitination and enrichment of proteins important for lipid transport and metabolism. The aim of this study was therefore to elucidate the role of neuronal lipids in BMAA-induced neurodegeneration...
December 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27942804/selective-improvement-of-peptides-imaging-on-tissue-by-supercritical-fluid-wash-of-lipids-for-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#19
Shoko Matsushita, Noritaka Masaki, Kohei Sato, Takahiro Hayasaka, Eiji Sugiyama, Shu-Ping Hui, Hitoshi Chiba, Nobuyuki Mase, Mitsutoshi Setou
There is a high analytical demand for improving the detection sensitivity for various peptides in matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) because exhaustive distribution analyses of various peptides could help to reveal the function of peptides in vivo. To improve the sensitivity of peptide detection, we used supercritical fluid of CO2 (scCO2) as washing solvent for a pretreatment to remove lipids. We evaluated whether our wash method using scCO2 with an entrainer improved the detection of peptides and suppressed lipid detection in MALDI-IMS...
December 9, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27939604/antigen-retrieval-prior-to-on-tissue-digestion-of-formalin-fixed-paraffin-embedded-tumour-tissue-sections-yields-oxidation-of-proline-residues
#20
Marie-Claude Djidja, Emmanuelle Claude, Peter Scriven, David W Allen, Vikki A Carolan, Malcolm R Clench
MALDI-mass spectrometry imaging (MALDI-MSI) has been shown to allow the study of protein distribution and identification directly within formalin-fixed paraffin-embedded (FFPE) tissue sections. However, direct protein identification from tissue sections remains challenging due to signal interferences and/or existing post-translational or other chemical modifications. The use of antigen retrieval (AR) has been demonstrated for unlocking proteins prior to in situ enzymatic digestion and MALDI-MSI analysis of FFPE tissue sections...
December 8, 2016: Biochimica et Biophysica Acta
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