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MALDI Imaging

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https://www.readbyqxmd.com/read/27890680/the-putative-role-of-maldi-msi-in-the-study-of-membranous-nephropathy
#1
Andrew Smith, Vincenzo L'Imperio, Elena Ajello, Franco Ferrario, Niccolò Mosele, Martina Stella, Manuel Galli, Clizia Chinello, Federico Pieruzzi, Goce Spasovski, Fabio Pagni, Fulvio Magni
Membranous Nephropathy (MN) is an immunocomplex mediated renal disease that represents the leading cause of nephrotic syndrome in adults and is one of the most frequent glomerulopathies worldwide. This glomerular disease can manifest as primary (idiopathic) or secondary and this distinction is crucial when choosing the most appropriate management of patients. In secondary cases, the best strategy consists in treating the underlying disease whereas in primary forms, the possible identification of confirmatory markers of the idiopathic etiology underlining the process is requested by clinicians...
November 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27890679/evaluation-of-non-supervised-maldi-mass-spectrometry-imaging-combined-with-microproteomics-for-glioma-grade-iii-classification
#2
Emilie Le Rhun, Marie Duhamel, Maxence Wisztorski, Jean-Pascal Gimeno, Fahed Zairi, Fabienne Escande, Nicolas Reyns, Firas Kobeissy, Claude-Alain Maurage, Michel Salzet, Isabelle Fournier
An integrated diagnosis using molecular features is recommended in the 2016 World Health Organization (WHO) classification. Our aim was to explore non-targeted molecular classification using MALDI mass spectrometry imaging (MALDI MSI) associated to microproteomics in order to classify anaplastic glioma by integration of clinical data. We used fresh-frozen tissue sections to perform MALDI MSI of proteins based on their digestion peptides after in-situ trypsin digestion of the tissue sections and matrix deposition by micro-spraying...
November 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27888142/profiling-and-identification-of-new-proteins-involved-in-brain-ischemia-using-maldi-imaging-mass-spectrometry
#3
Víctor Llombart, Sebastián Alejandro Trejo, Sílvia Bronsoms, Anna Morancho, Ma Feifei, Júlia Faura, Teresa García-Berrocoso, Alba Simats, Anna Rosell, Francesc Canals, Mar Hernández-Guillamón, Joan Montaner
: The identification of proteins involved in brain ischemia might allow the discovery of putative biomarkers or therapeutic targets for ischemic stroke. Our aim is to study the distribution of proteins within mouse brain after an ischemic insult using MALDI imaging-mass-spectrometry and to identify relevant proteins involved in brain damage. We occluded the middle cerebral artery of C57BL/6J mice. Brain slices were analyzed by MALDI-TOF and infarct (IC) and contralateral (CL) regions were compared using ClinProTools...
November 22, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27885266/electrospray-deposition-device-used-to-precisely-control-the-matrix-crystal-to-improve-the-performance-of-maldi-msi
#4
Shilei Li, Yangyang Zhang, Jian'an Liu, Juanjuan Han, Ming Guan, Hui Yang, Yu Lin, Shaoxiang Xiong, Zhenwen Zhao
MALDI MSI has been recently applied as an innovative tool for detection of molecular distribution within a specific tissue. MALDI MSI requires deposition of an organic compound, known as matrix, on the tissue of interest to assist analyte desorption and ionization, in which the matrix crystal homogeneity and size greatly influence the imaging reproducibility and spatial resolution in MALDI MSI. In this work, a homemade electrospray deposition device was developed for deposition of matrix in MALDI MSI. The device could be used to achieve 1 μm homogeneous matrix crystals in MALDI MSI analysis...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27883030/spatial-quantitation-of-drugs-in-tissues-using-liquid-extraction-surface-analysis-mass-spectrometry-imaging
#5
John G Swales, Nicole Strittmatter, James W Tucker, Malcolm R Clench, Peter J H Webborn, Richard J A Goodwin
Liquid extraction surface analysis mass spectrometry imaging (LESA-MSI) has been shown to be an effective tissue profiling and imaging technique, producing robust and reliable qualitative distribution images of an analyte or analytes in tissue sections. Here, we expand the use of LESA-MSI beyond qualitative analysis to a quantitative analytical technique by employing a mimetic tissue model previously shown to be applicable for MALDI-MSI quantitation. Liver homogenate was used to generate a viable and molecularly relevant control matrix for spiked drug standards which can be frozen, sectioned and subsequently analyzed for the generation of calibration curves to quantify unknown tissue section samples...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27873216/prognostic-metabolite-biomarkers-for-soft-tissue-sarcomas-discovered-by-mass-spectrometry-imaging
#6
Sha Lou, Benjamin Balluff, Arjen H G Cleven, Judith V M G Bovée, Liam A McDonnell
Metabolites can be an important read-out of disease. The identification and validation of biomarkers in the cancer metabolome that can stratify high-risk patients is one of the main current research aspects. Mass spectrometry has become the technique of choice for metabolomics studies, and mass spectrometry imaging (MSI) enables their visualization in patient tissues. In this study, we used MSI to identify prognostic metabolite biomarkers in high grade sarcomas; 33 high grade sarcoma patients, comprising osteosarcoma, leiomyosarcoma, myxofibrosarcoma, and undifferentiated pleomorphic sarcoma were analyzed...
November 21, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27873003/localization-of-ergot-alkaloids-in-sclerotia-of-claviceps-purpurea-by-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
#7
Julian Dopstadt, Simeon Vens-Cappell, Lisa Neubauer, Paul Tudzynski, Benedikt Cramer, Klaus Dreisewerd, Hans-Ulrich Humpf
The fungus Claviceps purpurea produces highly toxic ergot alkaloids and accumulates these in the hardened bodies of fungal mycelium. These so-called sclerotia, or ergot bodies, replace the crop seed of infected plants, which can include numerous important food- and feedstuff such as rye and wheat. While several studies have explored details of the infection process and development of ergot bodies, little information is available on the spatial distribution of the mycotoxins in the sclerotia. Here we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) at a lateral resolution of 35 μm to visualize the distribution of two representative alkaloids, ergocristine and ergometrine, produced by Ecc93 and Gal 310 variants of C...
November 21, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27859865/high-spatial-resolution-mass-spectrometry-imaging-reveals-the-genetically-programmed-developmental-modification-of-the-distribution-of-thylakoid-membrane-lipids-among-individual-cells-of-the-maize-leaf
#8
Maria Emilia Dueñas, Adam T Klein, Liza E Alexander, Marna D Yandeau-Nelson, Basil J Nikolau, Young Jin Lee
Metabolism in plants is compartmentalized among different tissues, cells and subcellular organelles. Mass spectrometry imaging (MSI) with matrix-assisted laser desorption ionization (MALDI) has recently advanced to allow for the visualization of metabolites at single cell resolution. Here we applied 5 and 10 μm high-spatial resolution MALDI-MSI to the asymmetric Kranz anatomy of maize leaves to study the differential localization of two major anionic lipids in thylakoid membranes, sulfoquinovosyldiacylglycerols (SQDG) and phosphatidylglycerols (PG)...
November 17, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27843502/molecular-mapping-alzheimer-s-disease-maldi-imaging-of-formalin-fixed-paraffin-embedded-human-hippocampal-tissue
#9
Andrea R Kelley, George Perry, Chloe Bethea, Rudolph J Castellani, Stephan B H Bach
A method for the molecular mapping of formalin-fixed, paraffin-embedded human hippocampal tissue affected by Alzheimer's disease (AD) is presented. This approach utilizes imaging mass spectrometry (IMS) with matrix-assisted laser desorption/ionization (MALDI). The usefulness of this technique in comparing diseased versus nor mal tissue at the molecular level while continuing to maintain topological and morphological integrity is evident in the preliminary findings. The critical correlation of the deparaffination, washing, matrix deposition, and analysis steps in handling the tissue sections and how these steps impact the successful mapping of human hippocampal tissue is clearly demonstrated...
2016: Open Neurology Journal
https://www.readbyqxmd.com/read/27836618/a-new-classification-method-for-maldi-imaging-mass-spectrometry-data-acquired-on-formalin-fixed-paraffin-embedded-tissue-samples
#10
Tobias Boskamp, Delf Lachmund, Janina Oetjen, Yovany Cordero Hernandez, Dennis Trede, Peter Maass, Rita Casadonte, Jörg Kriegsmann, Arne Warth, Hendrik Dienemann, Wilko Weichert, Mark Kriegsmann
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) shows a high potential for applications in histopathological diagnosis, and in particular for supporting tumor typing and subtyping. The development of such applications requires the extraction of spectral fingerprints that are relevant for the given tissue and the identification of biomarkers associated with these spectral patterns. We propose a novel data analysis method based on the extraction of characteristic spectral patterns (CSPs) that allow automated generation of classification models for spectral data...
November 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27835937/fractional-laser-assisted-topical-delivery-leads-to-enhanced-accelerated-and-deeper-cutaneous-5-fluorouracil-uptake
#11
Emily Wenande, Uffe H Olesen, Mette M B Nielsen, Christian Janfelt, Steen Honoré Hansen, Rox Anderson, Merete Haedersdal
BACKGROUND: Topical 5-Fluorouracil (5-FU) exhibits suboptimal efficacy for non-melanoma skin cancer, attributed to insufficient intracutaneous penetration. This study investigates the impact of ablative fractional laser (AFXL) at different laser-channel depths on cutaneous 5-FU pharmacokinetics and biodistribution. METHODS: In vitro porcine skin underwent AFXL-exposure using a fractional 10,600nm CO2-laser, generating microscopic ablation zones (MAZ) reaching the dermoepidermal junction (MAZ-ED), superficial-(MAZ-DS), or mid-dermis(MAZ-DM)...
November 11, 2016: Expert Opinion on Drug Delivery
https://www.readbyqxmd.com/read/27831956/identifying-diffuse-axonal-injury-by-matrix-assisted-laser-desorption-ionization-time-of-flight-a-new-method-for-pathological-observation
#12
Guanheng Ren, Donghua Zou, Ping Huang, Zhengdong Li, Yu Shao, Kaifei Deng, Yijiu Chen, Ningguo Liu
PURPOSE: The aim of this study was to identify the diffuse axonal injury (DAI) of rat through screening out differentially expressed proteins which may represent potential biomarkers by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) imaging mass spectrometry (IMS), which could make a contribution for the research of forensic pathology. METHODS: A total of 15 brainstem sections of rats (10 with and 5 without DAI) were conducted by MALDI-TOF IMS...
December 2016: American Journal of Forensic Medicine and Pathology
https://www.readbyqxmd.com/read/27815610/matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-reveals-changes-of-phospholipid-distribution-in-induced-pluripotent-stem-cell-colony-differentiation
#13
Yasuo Shimizu, Motoyasu Satou, Keitaro Hayashi, Yusuke Nakamura, Mio Fujimaki, Yasuhiro Horibata, Hiromi Ando, Taiji Watanabe, Taichi Shiobara, Kazuyuki Chibana, Akihiro Takemasa, Hiroyuki Sugimoto, Naohiko Anzai, Yoshiki Ishii
Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS). Analysis revealed changes of the intensity and distribution of peaks at m/z 782.5 and 798.5 in iPSC colonies during spontaneous differentiation...
November 4, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27810414/maldi-imaging-mass-spectrometry-from-bench-to-bedside
#14
REVIEW
Kristina Schwamborn, Mark Kriegsmann, Wilko Weichert
Today, pathologists face many challenges in defining the precise morphomolecular diagnosis and in guiding clinicians to the optimal patients' treatment. To achieve this goal, increasingly, classical histomorphological methods have to be supplemented by high throughput molecular assays. Since MALDI imaging mass spectrometry (IMS) enables the assessment of spatial molecular arrangements in tissue sections, it goes far beyond microscopy in providing hundreds of different molecular images from a single scan without the need of target-specific reagents...
October 31, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27807892/matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-for-the-spatial-location-of-feline-oviductal-proteins
#15
M Apparicio, V G Santos, Dfo Rocha, C R Ferreira, B I Macente, G M Magalhães, A E Alves, T F Motheo, L C Padilha-Nakaghi, E A Pires-Buttler, G C Luvoni, M N Eberlin, Wrr Vicente
With the purpose of identifying factors involved in early stages of embryo development in the domestic cat, matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was used for the first time to describe the spatial localization of proteins in the oviducts of queens. Oviducts were obtained from two 2 and 4 years old cross-bred queens, divided into three segments, snap-frozen in liquid nitrogen and then stored at -80°C until use. Next, they were sectioned in a cryostat, fixed on ITO (indium tin oxide) conductive glass slides for MALDI-IMS and serial sections were collected on microscope slides for histology...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27800655/relationship-between-hmf-intake-and-smf-formation-in-vivo-an-animal-and-human-study
#16
Silvia Pastoriza de la Cueva, Juana Álvarez, Ákos Végvári, Javier Montilla-Gómez, Olga Cruz-López, Cristina Delgado-Andrade, José A Rufián-Henares
SCOPE: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by non-enzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF, or the formation of DNA adducts in animals or human beings. METHODS AND RESULTS: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF...
November 1, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27796322/high-resolution-maldi-mass-spectrometry-imaging-of-gallotannins-and-monoterpene-glucosides-in-the-root-of-paeonia-lactiflora
#17
Bin Li, Dhaka Ram Bhandari, Andreas Römpp, Bernhard Spengler
High-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) at 10 μm pixel size was performed to unravel the spatio-chemical distribution of major secondary metabolites in the root of Paeonia lactiflora. The spatial distributions of two major classes of bioactive components, gallotannins and monoterpene glucosides, were investigated and visualized at the cellular level in tissue sections of P. lactiflora roots. Accordingly, other primary and secondary metabolites were imaged, including amino acids, carbohydrates, lipids and monoterpenes, indicating the capability of untargeted localization of metabolites by using high-resolution MSI platform...
October 31, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27791282/differentiating-tumor-heterogeneity-in-ffpe-prostate-adenocarcinoma-tissues-using-pca-analysis-of-maldi-ims-spectral-data
#18
Irene Panderi, Evgeny Yakirevich, Silvana Papagerakis, Lelia Noble, Kara Lombardo, Dionysios Pantazatos
RATIONALE: Many patients with adenocarcinoma of the prostate present with advanced and metastatic cancer at the time of diagnosis. There is an urgent need to detect biomarkers that will improve the diagnosis and prognosis of this disease. MALDI imaging mass spectrometry (MALDI-IMS) is playing a key role in cancer research and it can be useful to unravel the molecular profile of prostate cancer biopsies. METHODS: MALDI imaging data sets are highly complex and their interpretation requires the use of multivariate statistical methods...
October 28, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27760751/phospholipid-profile-and-distribution-in-the-receptive-oviduct-and-uterus-during-early-diestrus-in-cattle
#19
Katia Roberta A Belaz, Alessandra Tata, Moana R França, Maressa I Santos da Silva, Pedro Henrique Vendramini, Anna Maria A P Fernandes, Fábio L D'Alexandri, Marcos N Eberlin, Mario Binelli
Phospholipid metabolism and signaling influences on early pregnancy events in cattle are unknown. This study aimed to characterize global phospholipid composition of oviduct and uterus during early diestrus in a model of contrasting embryo receptivity. Beef cows were treated to ovulate a larger (LF-LCL group, associated with greater receptivity) or smaller follicle (SF-SCL group) and, consequently, to present greater or smaller plasma concentrations of estradiol during proestrus-estrus as well as progesterone during early diestrus...
October 19, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27760391/molecular-profiles-of-thyroid-cancer-subtypes-classification-based-on-features-of-tissue-revealed-by-mass-spectrometry-imaging
#20
Monika Pietrowska, Hanna C Diehl, Grzegorz Mrukwa, Magdalena Kalinowska-Herok, Marta Gawin, Mykola Chekan, Julian Elm, Grzegorz Drazek, Anna Krawczyk, Dariusz Lange, Helmut E Meyer, Joanna Polanska, Corinna Henkel, Piotr Widlak
Determination of the specific type of thyroid cancer is crucial for the prognosis and selection of treatment of this malignancy. However, in some cases appropriate classification is not possible based on histopathological features only, and it might be supported by molecular biomarkers. Here we aimed to characterize molecular profiles of different thyroid malignancies using mass spectrometry imaging (MSI) which enables the direct annotation of molecular features with morphological pictures of an analyzed tissue...
October 17, 2016: Biochimica et Biophysica Acta
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