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https://www.readbyqxmd.com/read/28912484/evaluation-of-therapeutic-potential-of-the-silver-silver-chloride-nanoparticles-synthesized-with-the-aqueous-leaf-extract-of-rumex-acetosa
#1
Sobha Kota, Pradeep Dumpala, Ratna Kumari Anantha, Mahendra Kumar Verma, Surendranath Kandepu
Silver nanoparticles were green synthesized with the aqueous leaf extract of the widely consumed green leafy vegetable, Rumex acetosa (sorrel) and the obtained silver nanoparticles (Ag NPs) were tested for their in vitro antioxidant potential, cytotoxicity against human osteosarcoma (HOS) cell lines and antibacterial effects against sixteen human pathogenic clinical isolates. Different analytical techniques viz. UV-vis, FTIR, XRD, SEM-EDX and TEM were employed to characterize the synthesized Ag NPs. Surface Plasmon spectra for the Ag NPs with brownish black color were centered approximately at 448 nm...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28891632/design-of-boron-nitride-gelatin-electrospun-nanofibers-for-bone-tissue-engineering
#2
Sakthivel Nagarajan, Habib Belaid, Celine Pochat-Bohatier, Catherine Teyssier, Igor Iatsunskyi, Emerson Coy, Sebastien Balme, David Cornu, Philippe Miele, Narayana S Kalkura, Vincent Cavailles, Mikhael Bechelany
Gelatin is a biodegradable biopolymer obtained by collagen denaturation, which shows poor mechanical properties. Hence, improving its mechanical properties is very essential towards the fabrication of efficient nontoxic material for biomedical applications. For this aim, various methods are employed using external fillers such as ceramics or bioglass. In this report, we introduce boron nitride (BN) reinforced gelatin as a new class of two dimensional biocompatible nanomaterials. The effect of the nanofiller on the mechanical behavior is analyzed...
September 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28870899/effective-metabolic-targeting-of-human-osteosarcoma-cells-in-vitro-and-in-orthotopic-nude-mouse-models-with-recombinant-methioninase
#3
Kentaro Igarashi, Kei Kawaguchi, Tasuku Kiyuna, Kentaro Miyake, Takashi Murakami, Norio Yamamoto, Katsuhiro Hayashi, Hiroaki Kimura, Shinji Miwa, Hiroyuki Tsuchiya, Robert M Hoffman
BACKGROUND: Methionine dependence may be the only known general metabolic defect in cancer. In order to exploit methionine dependence for therapy, our laboratory previously cloned L-methionine α-deamino-γ-mercaptomethane lyase [EC 4.4.1.11]) (recombinant methioninase [rMETase]), which was subsequently tested in mouse models of various types of human tumors. The present study aimed to investigate the efficacy of rMETase on human osteosarcoma cells in vitro and in vivo. MATERIALS AND METHODS: Human osteosarcoma cell lines 143B, HOS and SOSN2 were tested in vitro for survival during a 72-h exposure to rMETase using the WST-8 assay...
September 2017: Anticancer Research
https://www.readbyqxmd.com/read/28854422/effects-of-the-long-non-coding-rna-host2-on-the-proliferation-migration-invasion-and-apoptosis-of-human-osteosarcoma-cells
#4
Wei Wang, Xiao Li, Fan-Bin Meng, Zhen-Xing Wang, Ren-Tao Zhao, Chun-Yang Yang
BACKGROUND/AIMS: This study aimed to explore the effects of the long non-coding RNA HOST2 (lnc-HOST2) on the proliferation, migration, invasion and apoptosis of osteosarcoma cells. METHODS: Osteosarcoma tissues and adjacent normal tissues from 52 patients were selected. Human osteosarcoma cell lines (SaOS2, HOS, U2OS and MG-63) were collected and cultured; MG-63 cells had the highest lnc-HOST2 expression and thus were used in subsequent experiments. Then, MG-63 cells were transfected and divided into the blank (no transfection), si-CON (transfected with negative control siRNA) and si-lnc-HOST2 (transfected with small interference lnc-HOST2 siRNA) groups...
August 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28749244/radiation-protection-and-mitigation-potential-of-phenylbutyrate-delivered-via-oral-administration
#5
Alexandra C Miller, Rafael Rivas, Robert McMahon, Karvelisse Miller, Leonard Tesoro, Vilmar Villa, Daminik Yanushkevich, Paul Lison
PURPOSE: Phenylbutyrate (PB), a histone deacetylase inhibitor (HDACi) has demonstrated radiation protection in both in vitro and in vivo models. Studies previously demonstrated that PB and other HDAC inhibitors could inhibit radiation lethality in vivo by subcutaneous (s.c) injection. The objective of this study was to test the ability of oral PB treatment to protect against or to mitigate acute gamma radiation-induced lethality in vivo. MATERIALS AND METHODS: Human osteoblasts cells were used to evaluate radiation survival when PB was delivered pre- or post-radiation...
July 27, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28741397/survival-and-prognostic-factors-at-time-of-diagnosis-in-high-grade-appendicular-osteosarcoma-a-21-year-single-institution-evaluation-from-east-denmark
#6
Thomas Colding-Rasmussen, Andrea Pohly Thorn, Peter Horstmann, Catherine Rechnitzer, Lisa Lyngsie Hjalgrim, Anders Krarup-Hansen, Michael Mørk Petersen
BACKGROUND: Survival of patients with high-grade osteosarcoma (HOS), the most common primary bone cancer, has not improved significantly the last 30 years and the disease remains a major challenge. The purpose of this study is to evaluate survival in relation to prognostic factors at time of diagnosis among patients diagnosed with primary appendicular HOS in East Denmark between 1990 and 2010. MATERIAL AND METHODS: 101 patients (median age = 20 years, female/male ratio = 56/45) diagnosed with primary appendicular high-grade osteosarcoma between 1990 and 2010 were included in this study...
July 25, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28731779/arthroscopic-labral-base-repair-in-the-hip-5-year-minimum-clinical-outcomes
#7
Benjamin G Domb, Leslie C Yuen, Victor Ortiz-Declet, Jody Litrenta, Itay Perets, Austin W Chen
BACKGROUND: Arthroscopic labral base repair (LBR) in the hip is a previously described technique designed to restore the native functional anatomy of the labrum by reproducing its seal against the femoral head. LBR has been shown to have good short-term outcomes. Hypothesis/Purpose: The purpose was to evaluate clinical outcomes of an LBR cohort with a minimum 5-year follow-up. It was hypothesized that patients who underwent LBR would continue to have significant improvement from their preoperative scores and maintain scores similar to their 2-year outcomes...
July 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28716598/effect-of-lecithin-nanoliposome-or-soybean-lecithin-supplemented-by-pomegranate-extract-on-post-thaw-flow-cytometric-microscopic-and-oxidative-parameters-in-ram-semen
#8
Mahdieh Mehdipour, Hossein Daghigh Kia, Maryam Nazari, Abouzar Najafi
This investigation was carried out to study the effect of soybean lecithin 1.5% (wt/vol) (0, 2.5, 5 and 7.5 mg l(-1) pomegranate extract (PE)) or PE-loaded lecithin nanoliposome (0, 2.5, 5 and 7.5 mg l(-1)) to Tris-based extender. Sperm motility (CASA), viability, membrane integrity (HOS test), abnormalities, mitochondrial activity, apoptosis status, lipid peroxidation, total antioxidant capacity (TAC)) and antioxidant activities (GPX, SOD) were investigated following freeze-thawing. No significant differences were detected in motility parameters, viability, membrane integrity, and mitochondria activity after thawing sperm between soybean lecithin and lecithin nanoliposomes...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28662469/molecular-cellular-and-pharmacological-effects-of-platinum-ii-diiodido-complexes-containing-9-deazahypoxanthine-derivatives-a-group-of-broad-spectrum-anticancer-active-agents
#9
Ján Vančo, Zdeněk Trávníček, Radka Křikavová, Jana Gáliková, Zdeněk Dvořák, Marta Chalupová
The platinum(II) iodido complexes 1-5 of the general formula cis-[PtI2(Ln)2], where Ln stands for O-substituted 9-deazahypoxanthine derivatives, were prepared and thoroughly characterized by various techniques, including multinuclear 1D and 2D NMR spectroscopy. The complexes were screened for their anticancer potential in vitro on ten human cancer cell lines, concretely breast adenocarcinoma (MCF7), osteosarcoma (HOS), lung carcinoma (A549), cervix epithelioid carcinoma (HeLa), malignant melanoma (G-361), prostate carcinoma (22Rv1, PC-3), hepatocellular carcinoma (HepG2), ovarian carcinoma (A2780) and cisplatin-resistant ovarian carcinoma (A2780R)...
June 19, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28646317/di-n-butyl-phthalate-prompts-interruption-of-spermatogenesis-steroidogenesis-and-fertility-associated-with-increased-testicular-oxidative-stress-in-adult-male-rats
#10
Giribabu Nelli, Sreenivasula Reddy Pamanji
Di-n-butyl phthalate (DBP) is extensively used as plasticizer, and it was ubiquitary released into the environment. The present study was aimed to investigate the effect of DBP on reproductive competence in adult male rats. Adult male rats were received corn oil or DBP injection intraperitoneally (ip) at 100 and 500 mg/kg body weight on 90, 97, 104, and 111 days. Following completion of the experimental period, adult male rats were cohabitated with untreated proestrus female rats for determination of fertilization capacity...
June 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28632588/survivorship-and-outcomes-10-years-following-hip-arthroscopy-for-femoroacetabular-impingement-labral-debridement-compared-with-labral-repair
#11
COMPARATIVE STUDY
Travis J Menge, Karen K Briggs, Grant J Dornan, Shannen C McNamara, Marc J Philippon
BACKGROUND: Studies have demonstrated hip arthroscopy to be an effective treatment for femoroacetabular impingement (FAI) with associated labral tears. The purposes of this study were to report 10-year outcomes and hip survival following hip arthroscopy for FAI and to compare labral debridement with labral repair. METHODS: Prospectively collected data on patients followed for a minimum of 10 years after hip arthroscopy for FAI with either labral debridement or labral repair performed by a single surgeon were retrospectively analyzed...
June 21, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28630723/outcome-after-arthroscopic-labral-surgery-in-patients-previously-treated-with-periacetabular-osteotomy-a-follow-up-study-of-43-patients
#12
Charlotte Hartig-Andreasen, Torsten G Nielsen, Bent Lund, Kjeld Søballe, Martin Lind
To identify factors predicting failure after hip arthroscopy in patients with previous periacetabular osteotomy (PAO) defined as a conversion to total hip replacement (THR) and to evaluate the patient reported outcome scores. Of 55 hips treated with hip arthroscopy after PAO from Aug 2008 to 2012 at Aarhus University Hospital, 43 hips were included (median age: 36.1 yrs, range 16.3-56.9 yrs). Indications were unacceptable pain, a positive FABER and impingement test and signs of labral damage on MR-arthrography...
January 2017: Journal of Hip Preservation Surgery
https://www.readbyqxmd.com/read/28602527/evidence-for-reliability-validity-and-responsiveness-of-turkish-version-of-hip-outcome-score
#13
Gökhan Polat, Derya Çelik, Hilal Çil, Mehmet Erdil, Mehmet Aşık
BACKGROUND: Hip Outcome Score (HOS), originally developed in English, assesses the severity of hip pathology. To date, no Turkish version of the questionnaire exists. PURPOSE: The aim of our study was to translate the HOS into Turkish and verify its psychometric properties. METHODS: The translation and cultural adaptation were performed according to international recommendations in five stages: The HOS was translated into Turkish, consistent with published methodological guidelines...
June 8, 2017: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/28588489/fh535-suppresses-osteosarcoma-growth-in-vitro-and-inhibits-wnt-signaling-through-tankyrases
#14
Carl T Gustafson, Tewodros Mamo, Kristen L Shogren, Avudaiappan Maran, Michael J Yaszemski
Osteosarcoma (OS) is an aggressive primary bone tumor which exhibits aberrantly activated Wnt signaling. The canonical Wnt signaling cascade has been shown to drive cancer progression and metastasis through the activation of β-catenin. Hence, small molecule inhibitors of Wnt targets are being explored as primary or adjuvant chemotherapy. In this study, we have investigated the ability of FH535, an antagonist of Wnt signaling, to inhibit the growth of OS cells. We found that FH535 was cytotoxic in all OS cell lines which were tested (143b, U2OS, SaOS-2, HOS, K7M2) but well tolerated by normal human osteoblast cells...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28534278/the-effect-of-antioxidant-agents-addition-and-freezing-method-on-quality-parameters-of-frozen-thawed-ram-semen
#15
Leonidas Vichas, Ioannis A Tsakmakidis, Dimitrios Vafiadis, Georgios Tsousis, Eleni Malama, Constantin M Boscos
The aim of this study was to evaluate the effect of antioxidant agents and freezing methods on the ability of ram sperm to preserve its post-thaw quality characteristics. Six Chios rams were subjected to 52 weekly semen collections. Each ram was used as semen donor for freezing experiments once every 2 weeks. Equal number of good quality spermatozoa from each ejaculate (concentration ≥1 × 10(9) spermatozoa/ml, motility ≥70%, motility score ≥3.5) were pooled. Three equal aliquots of the pooled sample were diluted using three different fractions of a milk-based and glycerol extender (control, quercetin-enriched, α-tocopherol-enriched)...
May 22, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28516234/assessing-long-term-return-to-play-after-hip-arthroscopy-in-football-players-evaluating-risk-factors-for-good-prognosis
#16
D Barastegui, R Seijas, P Alvarez-Diaz, E Rivera, E Alentorn-Geli, G Steinbacher, X Cuscó, R Cugat
PURPOSE: Groin pain is the third most common disease in football players and has often been associated with hip pathology such as femoroacetabular impingement and labral lesions. Hip arthroscopy offers possibilities of function restoration via minimally invasive procedures. The aim of this study is to evaluate professional football player's injuries and their return to play after hip arthroscopy for FAI and labral injuries. METHODS: Patients that underwent hip arthroscopy between 2009 and 2014 were selected retrospectively...
May 17, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28473879/dihydropyridine-derivatives-as-cell-growth-modulators-in-vitro
#17
Imanta Bruvere, Egils Bisenieks, Janis Poikans, Janis Uldrikis, Aiva Plotniece, Karlis Pajuste, Martins Rucins, Brigita Vigante, Zenta Kalme, Marina Gosteva, Ilona Domracheva, Astrida Velena, Tea Vukovic, Lidija Milkovic, Gunars Duburs, Neven Zarkovic
The effects of eleven 1,4-dihydropyridine derivatives (DHPs) used alone or together with prooxidant anticancer drug doxorubicin were examined on two cancer (HOS, HeLa) and two nonmalignant cell lines (HMEC, L929). Their effects on the cell growth ((3)H-thymidine incorporation) were compared with their antiradical activities (DPPH assay), using well-known DHP antioxidant diludine as a reference. Thus, tested DHPs belong to three groups: (1) antioxidant diludine; (2) derivatives with pyridinium moieties at position 4 of the 1,4-DHP ring; (3) DHPs containing cationic methylene onium (pyridinium, trialkylammonium) moieties at positions 2 and 6 of the 1,4-DHP ring...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28466479/selection-of-viable-human-spermatozoa-with-low-levels-of-dna-fragmentation-from-an-immotile-population-using-density-gradient-centrifugation-and-magnetic-activated-cell-sorting
#18
H Zhang, X Xuan, S Yang, X Li, C Xu, X Gao
We aimed to determine whether density gradient centrifugation and magnetic-activated cell sorting (DGC-MACS) could select viable spermatozoa, with lower levels of DNA fragmentation, from an immotile population. Analysis involved sixteen patients, each with a sperm count ≥10(7) /mL. All samples were immotile despite exhibiting a live population >40%. Spermatozoa were prepared using DGC-MACS and selected spermatozoa evaluated for membrane and DNA integrity using the hypo-osmotic swelling (HOS) test, vital staining and the TUNEL test...
May 3, 2017: Andrologia
https://www.readbyqxmd.com/read/28409819/spermicidal-efficacy-of-vrp-a-synthetic-cationic-antimicrobial-peptide-inducing-apoptosis-and-membrane-disruption
#19
Prasanta Ghosh, Arpita Bhoumik, Sudipta Saha, Sandipan Mukherjee, Sarfuddin Azmi, Jimut K Ghosh, Sandhya R Dungdung
Presently available contraceptives are mostly hormonal or detergent in nature with numerous side effects like irritation, lesion, inflammation in vagina, alteration of body homeostasis, etc. Antimicrobial peptides with spermicidal activity but without adverse effects may be suitable alternatives. In the present study, spermicidal activity of a cationic antimicrobial peptide VRP on human spermatozoa has been elucidated. Progressive forward motility of human spermatozoa was instantly stopped after 100 μM VRP treatment and at 350 μM, all kinds of sperm motility ceased within 20 s as assessed by the Sander-Cramer assay...
April 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28302429/does-bony-regrowth-occur-after-arthroscopic-femoroplasty-in-a-group-of-young-adolescents
#20
Itay Perets, Asheesh Gupta, Edwin O Chaharbakhshi, Lyall Ashberg, David E Hartigan, Mary R Close, Benjamin G Domb
PURPOSE: To evaluate femoral head-neck bone regrowth and PROs in skeletally immature patients that underwent arthroscopic femoroplasties over a minimum 2-year period. METHODS: Eleven skeletally immature hips (10 patients) with open femoral head physes underwent femoroplasty between October 2008 and November 2013. Inclusion criteria were minimum 2-year follow-up with patient-reported outcomes (PROs) and radiographs preoperatively, at 2 weeks, and at a minimum of 2 years postoperatively...
May 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
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