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https://www.readbyqxmd.com/read/27860196/tricetin-inhibits-human-osteosarcoma-cells-metastasis-by-transcriptionally-repressing-mmp-9-via-p38-and-akt-pathways
#1
Pin-Yu Chang, Ming-Ju Hsieh, Yih-Shou Hsieh, Pei-Ni Chen, Jia-Sin Yang, Fang-Cheng Lo, Shun-Fa Yang, Ko-Hsiu Lu
Tricetin, a dietary flavonoid, has cytostatic properties and anti-metastasis activities in various cancer cells. However, the detailed impacts and underlying mechanisms of tricetin on human osteosarcoma cell metastasis are still unclear. Here, the hypothesis that tricetin possesses the anti-metastatic effects on human osteosarcoma cells was tested. The effects of tricetin on cell viability, motility, migration, and invasion in human osteosarcoma U2OS and HOS cells were investigated. Gelatin zymography, western blotting, polymerase chain reaction (PCR), and the luciferase assay were used to further explore the underlying mechanisms involved in anti-metastatic effects in U2OS cells...
November 8, 2016: Environmental Toxicology
https://www.readbyqxmd.com/read/27847489/how-do-maternal-subclinical-symptoms-influence-infant-motor-development-during-the-first-year-of-life
#2
Giulia Piallini, Stefania Brunoro, Chiara Fenocchio, Costanza Marini, Alessandra Simonelli, Marina Biancotto, Stefania Zoia
An unavoidable reciprocal influence characterizes the mother-child dyad. Within this relationship, the presence of depression, somatization, hostility, paranoid ideation, and interpersonal sensitivity symptoms at a subclinical level and their possible input on infant motor competences has not been yet considered. Bearing in mind that motor abilities represent not only an indicator of the infant's health-status, but also the principal field to infer his/her needs, feelings and intentions, in this study the quality of infants' movements were assessed and analyzed in relationship with the maternal attitudes...
2016: Frontiers in Psychology
https://www.readbyqxmd.com/read/27628727/an-automatic-prediction-of-epileptic-seizures-using-cloud-computing-and-wireless-sensor-networks
#3
Sanjay Sareen, Sandeep K Sood, Sunil Kumar Gupta
Epilepsy is one of the most common neurological disorders which is characterized by the spontaneous and unforeseeable occurrence of seizures. An automatic prediction of seizure can protect the patients from accidents and save their life. In this article, we proposed a mobile-based framework that automatically predict seizures using the information contained in electroencephalography (EEG) signals. The wireless sensor technology is used to capture the EEG signals of patients. The cloud-based services are used to collect and analyze the EEG data from the patient's mobile phone...
November 2016: Journal of Medical Systems
https://www.readbyqxmd.com/read/27579536/tumor-and-circulating-biomarkers-in-patients-with-second-line-hepatocellular-carcinoma-from-the-randomized-phase-ii-study-with-tivantinib
#4
Lorenza Rimassa, Giovanni Abbadessa, Nicola Personeni, Camillo Porta, Ivan Borbath, Bruno Daniele, Stefania Salvagni, Jean-Luc Van Laethem, Hans Van Vlierberghe, Jörg Trojan, Enrico N De Toni, Alan Weiss, Steven Miles, Antonio Gasbarrini, Monica Lencioni, Maria E Lamar, Yunxia Wang, Dale Shuster, Brian E Schwartz, Armando Santoro
: ARQ 197-215 was a randomized placebo-controlled phase II study testing the MET inhibitor tivantinib in second-line hepatocellular carcinoma (HCC) patients. It identified tumor MET as a key biomarker in HCC. Aim of this research was to study the prognostic and predictive value of tumor (MET, the receptor tyrosine kinase encoded by the homonymous MNNG-HOS transforming gene) and circulating (MET, hepatocyte growth factor [HGF], alpha-fetoprotein [AFP], vascular endothelial growth factor [VEGF]) biomarkers in second-line HCC...
August 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27487911/suberanilohydroxamic-acid-vorinostat-synergistically-enhances-the-cytotoxicity-of-doxorubicin-and-cisplatin-in-osteosarcoma-cell-lines
#5
Aleksandra Pettke, Marc Hotfilder, Dagmar Clemens, Stephanie Klco-Brosius, Christiane Schaefer, Jenny Potratz, Uta Dirksen
Osteosarcoma is the most common primary bone cancer in children and is a highly malignant disease, in which 25% of patients present with metastasis at diagnosis. Considerable advances in the treatment of localized disease have been achieved since the introduction of combined modality treatment, increasing the prognosis of overall survival to 70%. Yet, established therapies have only limited success in treating both metastatic disease and nonresponders to primary chemotherapy. Therefore, new therapeutic approaches are required, particularly for the control of osteosarcoma in these patient groups...
November 2016: Anti-cancer Drugs
https://www.readbyqxmd.com/read/27416992/are-short-term-outcomes-of-hip-arthroscopy-in-patients-55-years-and-older-inferior-to-those-in-younger-patients
#6
Andrew J Bryan, Aaron J Krych, Ayoosh Pareek, Patrick J Reardon, Rebecca Berardelli, Bruce A Levy
BACKGROUND: Hip arthroscopy for young patients with femoroacetabular impingement (FAI) has been successful, but the efficacy of hip arthroscopy in older patients is not clearly defined. PURPOSE: To evaluate the clinical outcomes of patients 55 years and older who are undergoing hip arthroscopy and to compare outcomes with those of patients younger than 55 years. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: A total of 201 (63 male, 138 female) patients undergoing primary hip arthroscopy for FAI without radiographic arthritis (Tönnis grade <3) were isolated from a prospective database and stratified by age to <55-year and ≥55-year groups...
July 14, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27328482/sperm-with-an-abnormal-hypo-osmotic-swelling-test-normal-fertilization-normal-embryo-development-but-implantation-failure
#7
EDITORIAL
J H Check, J Aly
PURPOSE: To review the clinical importance of including the hypo-osmotic swelling (HOS) test in routine male fertility testing which in general is not evaluated by most physicians dealing with infertility. MATERIALS AND METHODS: Pregnancy rates were evaluated in patients with low HOS test scores. A low HOS test was specifically defined as having less than 50% of sperm exhibiting the normal physiologic response of tail swelling, when subjected to a hypo-osmolar solution...
2016: Clinical and Experimental Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27274424/pre-and-post-operative-self-reported-function-and-quality-of-life-in-women-with-and-without-generalized-joint-laxity-undergoing-hip-arthroscopy-for-femoroacetabular-impingement
#8
Mattie Pontiff, Matthew P Ithurburn, Thomas Ellis, Kathleen Cenkus, Stephanie Di Stasi
BACKGROUND: Generalized joint laxity is more prevalent in women than men and may lead to poorer post-operative outcomes in select orthopedic populations. There are no studies examining peri-operative function in patients with generalized joint laxity (GJL) and femoroacetabular impingement (FAI). PURPOSE: The purpose of this study was to determine the difference in perceived function and quality of life as measured by the Hip Outcome Score ADL subscale (HOS-ADL), International Hip Outcomes Tool (iHOT-33) and the Short Form 12-Item Health Survey (SF-12) in women with and without GJL prior to and six months after undergoing hip arthroscopy for FAI...
June 2016: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/27259711/comparative-evaluation-of-two-fully-automated-real-time-pcr-methods-for-mrsa-admission-screening-in-a-tertiary-care-hospital
#9
N J Hos, P Wiegel, J Fischer, G Plum
We evaluated two fully-automated real-time PCR systems, the novel QIAGEN artus MRSA/SA QS-RGQ and the widely used BD MAX MRSA assay, for their diagnostic performance in MRSA admission screening in a tertiary-care university hospital. Two hundred sixteen clinical swabs were analyzed for MRSA DNA using the BD MAX MRSA assay. In parallel, the same specimens were tested with the QIAGEN artus MRSA/SA QS-RGQ. Automated steps included lysis of bacteria, DNA extraction, real-time PCR and interpretation of results. MRSA culture was additionally performed as a reference method for MRSA detection...
September 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27158569/knowledge-regarding-basic-facts-of-stroke-among-final-year-mbbs-students-and-house-officers-a-cross-sectional-survey-of-708-respondents-from-pakistan
#10
Mohammad U Khubaib, Farooq A Rathore, Ahmed Waqas, Mohsin M Jan, Sana Sohail
INTRODUCTION: Stroke is the leading cause of neurological disability in the world. In Pakistan, house officers (HOs) are usually the first contact for a stroke patient in the emergency department. Sometimes they need to make quick decisions regarding diagnosis and management without specialist supervision. Thousands of current final year MBBS (Bachelor of Medicine, Bachelor of Surgery) students will be performing the duties of HOs soon. This study documents the knowledge and confidence levels of final year students and HOs in Pakistan regarding basic facts related to initial diagnosis and management of stroke...
2016: Curēus
https://www.readbyqxmd.com/read/27139084/incidence-of-sperm-surface-autoantibodies-and-relationship-with-routine-semen-parameters-and-sperm-kinematics
#11
Gustavo Luis Verón, Rosa Isabel Molina, Andrea Daniela Tissera, Gustavo Martín Estofan, Clara Isabel Marín-Briggiler, Mónica Hebe Vazquez-Levin
PROBLEM: Antisperm antibodies (ASA) are associated with male subfertility. However, results on sperm surface autoantibodies are controversial, the relationship between ASA and semen parameters (WHO, 2010) is unknown, and data on ASA and sperm kinematics are scarce. METHOD OF STUDY: A retrospective study carried out in men undergoing routine semen analysis (WHO 2010), ASA evaluation (direct SpermMAR(™) (IgG) test), and computer-assisted sperm analysis (CASA). RESULTS: A 2...
July 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27051208/effect-of-management-system-and-season-on-semen-freezability-in-jakhrana-bucks
#12
Narendra Kumar, B Rai, Showkat A Bhat, S D Kharche, Chetna Gangwar, S K Jindal, Subhash Chandra
AIM: The objective of the study was to determine the effect of the management system (intensive and semi-intensive) and season (autumn and winter) on semen freezability in Jakhrana bucks. MATERIALS AND METHODS: A total of 24 Jakhrana bucks of same body weight and age (BW=30 kg, age=1 year) were randomly allotted into two groups, viz., Group I (intensive system, 12 bucks) and Group II (semi-intensive system, 12 bucks). These two groups were statistically tested for their homogeneity with respect to age and BW...
February 2016: Veterinary World
https://www.readbyqxmd.com/read/26957259/a-correlation-between-selenium-and-carnitine-levels-with-hypo-osmotic-swelling-test-for-sperm-membrane-in-low-grade-varicocele-patients
#13
B Pajovic, A Dimitrovski, N Radojevic, M Vukovic
OBJECTIVE: The hypo-osmotic swelling (HOS) test predicts membrane integrity by determining the ability of the sperm membrane to maintain equilibrium between the sperm cell and its environment. The aim of our study was to determine the correlation between selenium and carnitine levels in the seminal fluid with HOS test for sperm membrane in low-grade varicocele patients. PATIENTS AND METHODS: Study numbered 64 examinees who suffered from low-grade varicocele and were divided into two groups, according to fertility potential and HOS test outcome...
2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/26910666/opposite-effects-of-soluble-factors-secreted-by-adipose-tissue-on-proliferating-and-quiescent-osteosarcoma-cells
#14
COMPARATIVE STUDY
Pierre Avril, Franck Duteille, Perrine Ridel, Marie-Françoise Heymann, Gonzague De Pinieux, Françoise Rédini, Frédéric Blanchard, Dominique Heymann, Valérie Trichet, Pierre Perrot
BACKGROUND: Autologous adipose tissue transfer may be performed for aesthetic needs following resection of osteosarcoma, the most frequent primary malignant tumor of bone, excluding myeloma. The safety of autologous adipose tissue transfer regarding the potential risk of cancer recurrence must be addressed. METHODS: Adipose tissue injection was tested in a human osteosarcoma preclinical model induced by MNNG-HOS cells. Culture media without growth factors from fetal bovine serum were conditioned with adipose tissue samples and added to two osteosarcoma cell lines (MNNG-HOS and MG-63) that were cultured in monolayer or maintained in nonadherent spheres, favoring a proliferation or quiescent stage, respectively...
March 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/26845116/multiplex-diagnosis-of-oncogenic-fusion-and-met-exon-skipping-by-molecular-counting-using-formalin-fixed-paraffin-embedded-lung-adenocarcinoma-tissues
#15
Kuniko Sunami, Koh Furuta, Koji Tsuta, Shinji Sasada, Takehiro Izumo, Takashi Nakaoku, Yoko Shimada, Motonobu Saito, Hiroshi Nokihara, Shun-Ichi Watanabe, Yuichiro Ohe, Takashi Kohno
INTRODUCTION: Fusions of the anaplastic lymphoma receptor tyrosine kinase gene (ALK), ret proto-oncogene (RET), ROS proto-oncogene 1, receptor tyrosine kinase gene (ROS1), B-Raf proto-oncogene, serine/threonine kinase gene (BRAF), and neuregulin 1 gene (NRG1) and intronic MMNG HOS Transforming gene (MET) mutations are druggable oncogene alterations in lung adenocarcinoma that cause expression of aberrant transcripts. Because these aberrant transcripts are both infrequent (incidence <5%) and mutually exclusive, multiplex assays are required to detect them in tumor samples...
February 2016: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/26837622/prognostic-value-of-a-pre-freeze-hypo-osmotic-swelling-test-on-the-post-thaw-quality-of-dog-semen
#16
S Karger, B Geiser, M Grau, O Burfeind, W Heuwieser, S P Arlt
Throughout cryopreservation, sperm are exposed to major osmotic challenges. Only intact membranes of sperm cells are able to regulate these volumetric changes, which can be determined by the hypo-osmotic swelling test (HOS test). Correlations between the HOS test and conventional semen variables are inconsistent. Therefore, the objectives of this study were (1) to examine relationships between HOS test results and standard semen variables before freezing and after thawing and (2) to evaluate the prognostic value of the HOS assessments on post-thaw quality of dog semen...
March 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/26689751/blockade-of-ccr7-leads-to-decreased-dendritic-cell-migration-to-draining-lymph-nodes-and-promotes-graft-survival-in-low-risk-corneal-transplantation
#17
D Hos, J Dörrie, N Schaft, F Bock, M Notara, F E Kruse, S Krautwald, C Cursiefen, B O Bachmann
The chemokine receptor CCR7 is essential for migration of mature dendritic cells (DCs) to the regional lymph nodes, and it has been shown that blocking of CCR7 improves graft survival after high-risk corneal transplantation in vascularized recipient corneas. However, it is so far unknown whether blocking of CCR7 reduces migration of DCs from the avascular cornea to the draining lymph nodes and whether this leads to improved graft survival also in the low-risk setting of corneal transplantation, which accounts for the majority of perforating transplantations performed...
May 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/26679163/sperm-characteristics-following-freezing-in-extenders-supplemented-with-whole-egg-yolk-and-different-concentrations-of-low-density-lipoproteins-in-the-collared-peccary-pecari-tajacu
#18
Ana Liza Paz Souza, Gabriela Liberalino Lima, Gislayne Christianne Xavier Peixoto, Thibério de Souza Castelo, Maria Glaucia Carlos Oliveira, Valéria Veras de Paula, Alexandre Rodrigues Silva
The aim of the current study was to compare sperm quality characteristics of the collared peccary (Pecari tajacu) following freezing in extenders supplemented with whole egg yolk and different concentrations of low-density lipoproteins (LDL). Semen from 11 adult males was obtained by electroejaculation and evaluated for sperm motility, vigor, morphology as well as membrane integrity analyzed by the hypo-osmotic swelling (HOS) test and a fluorescent staining. Moreover, the semen was diluted in a Tris-based extender containing 20% egg yolk (control group) or 5, 10 or 20% LDL (treatment groups)...
December 2015: Reproductive Biology
https://www.readbyqxmd.com/read/26674513/pre-operative-low-back-pain-negatively-affects-self-reported-function-in-individuals-undergoing-hip-arthroscopy
#19
Lindsay C Becker, Stephanie Carter-Kelley, Thomas Ellis, Kathleen Cenkus, Stephanie L Di Stasi
BACKGROUND/PURPOSE: Low back pain (LBP) is a common source of disability in adults and highly prevalent in patients with painful hip pathology. Persistent LBP after hip arthroplasty is associated with lower self-reported function, however, the effect of pre-operative LBP in patients undergoing hip arthroscopy for FAI has not been evaluated. The purpose of this study was to determine whether improvements in self-reported hip function following arthroscopic surgery for femoroacetabular impingement (FAI) differed between those with and without reports of pre-operative low back pain...
December 2015: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/26610329/mesorhizobium-calcicola-sp-nov-mesorhizobium-waitakense-sp-nov-mesorhizobium-sophorae-sp-nov-mesorhizobium-newzealandense-sp-nov-and-mesorhizobium-kowhaii-sp-nov-isolated-from-sophora-root-nodules-in-new-zealand
#20
Sofie E De Meyer, Heng Wee Tan, Mitchell Andrews, Peter B Heenan, Anne Willems
In total, 31 strains of gram-negative, rod-shaped bacteria were isolated from Sophora root nodules and authenticated as rhizobia on this hos. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, with the representative strains ICMP 19560T, ICMP 19523T, ICMP 19535T, ICMP 19545T and ICMP 19512T being most closely related to Mesorhizobium sangaii SCAU7T (99.9 - 99.6%), Mesorhizobium cantuariense ICMP 19515T (99.7 - 99.6%) and Mesorhizobium ciceri UMP-CA7T (99.7 - 99.5%). Additionally, our strains formed distinct groups in the housekeeping gene analysis and were closely related to Mesorhizobium waimense ICMP 19557T (93...
November 26, 2015: International Journal of Systematic and Evolutionary Microbiology
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