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https://www.readbyqxmd.com/read/28409819/spermicidal-efficacy-of-vrp-a-synthetic-cationic-antimicrobial-peptide-inducing-apoptosis-and-membrane-disruption
#1
Prasanta Ghosh, Arpita Bhoumik, Sudipta Saha, Sandipan Mukherjee, Sarfuddin Azmi, Jimut Kanti Ghosh, Sandhya Rekha Dungdung
Presently available contraceptives are mostly hormonal or detergent in nature with numerous side effects like irritation, lesion, inflammation in vagina, alteration of body homeostasis etc. Antimicrobial peptides with spermicidal activity but without adverse effects may be suitable alternatives. In the present study spermicidal activity of a cationic antimicrobial peptide VRP on human spermatozoa has been elucidated. Progressive forward motility of human spermatozoa was instantly stopped after 100 µM VRP treatment and at 350 µM all kinds of sperm motility ceased within 20 seconds as assessed by the Sander-Cramer assay...
April 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28302429/does-bony-regrowth-occur-after-arthroscopic-femoroplasty-in-a-group-of-young-adolescents-femoroplasty-regrowth-in-adolescents
#2
Itay Perets, Asheesh Gupta, Edwin O Chaharbakhshi, Lyall Ashberg, David E Hartigan, Mary R Close, Benjamin G Domb
PURPOSE: To evaluate femoral head-neck bone regrowth and PROs in skeletally immature patients that underwent arthroscopic femoroplasties over a minimum 2-year period. METHODS: Eleven skeletally immature hips (10 patients) with open femoral head physes underwent femoroplasty between October 2008 and November 2013. Inclusion criteria were minimum 2-year follow-up with patient-reported outcomes (PROs) and radiographs preoperatively, at 2 weeks, and at a minimum of 2 years postoperatively...
March 13, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28246931/effects-of-sost-gene-silencing-on-proliferation-apoptosis-invasion-and-migration-of-human-osteosarcoma-cells-through-the-wnt-%C3%AE-catenin-signaling-pathway
#3
Jian Zou, Wei Zhang, Xiao-Lin Li
Our study explored the effects of SOST gene silencing on the proliferation, apoptosis, invasion, and migration of human osteosarcoma cells through Wnt/β-catenin signaling pathway. Fresh tissues were obtained from 108 patients with osteosarcoma and 46 patients with osteochondroma. Human osteosarcoma cells (MG-63, U2-OS, HOS, and Saos-2) and normal osteoblast (hFoB1.19) were selected and cultured. Osteosarcoma cells were grouped randomly into the blank group, the scrambled control group, and the SOST-siRNA group...
February 28, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28243577/evaluation-of-impact-of-anti-idursulfase-antibodies-during-long-term-idursulfase-enzyme-replacement-therapy-in-mucopolysaccharidosis-ii-patients
#4
R Giugliani, P Harmatz, S A Jones, N J Mendelsohn, A Vellodi, Y Qiu, C J Hendriksz, S Vijayaraghavan, D A H Whiteman, A Pano
OBJECTIVES: This 109-week, nonrandomized, observational study of mucopolysaccharidosis II (MPS II) patients already enrolled in the Hunter Outcome Survey (HOS) (NCT00882921), assessed the long-term immunogenicity of idursulfase, and examined the effect of idursulfase-specific antibody generation on treatment safety (via infusion-related adverse events [IRAEs]) and pharmacodynamics (via urinary glycosaminoglycans [uGAGs]). METHODS: Male patients ≥ 5 years, enrolled in HOS regardless of idursulfase treatment status were eligible...
September 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28204978/characterization-and-cytotoxic-effect-of-aqua-2-2-2-nitrilotriacetato-oxo-vanadium-salts-on-human-osteosarcoma-cells
#5
Aleksandra Tesmar, Dariusz Wyrzykowski, Rafał Kruszyński, Karolina Niska, Iwona Inkielewicz-Stępniak, Joanna Drzeżdżon, Dagmara Jacewicz, Lech Chmurzyński
The use of protonated N-heterocyclic compound, i.e. 2,2'-bipyridinium cation, [bpyH(+)], enabled to obtain the new nitrilotriacetate oxidovanadium(IV) salt of the stoichiometry [bpyH][VO(nta)(H2O)]H2O. The X-ray measurements have revealed that the compound comprises the discrete mononuclear [VO(nta)(H2O)](-) coordination ion that can be rarely found among other known compounds containing nitrilotriacetate oxidovanadium(IV) moieties. The antitumor activity of [bpyH][VO(nta)(H2O)]H2O and its phenanthroline analogue, [phenH][VO(nta)(H2O)](H2O)0...
April 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28163883/rules-to-fly-by-pigeons-navigating-horizontal-obstacles-limit-steering-by-selecting-gaps-most-aligned-to-their-flight-direction
#6
Ivo G Ros, Partha S Bhagavatula, Huai-Ti Lin, Andrew A Biewener
Flying animals must successfully contend with obstacles in their natural environments. Inspired by the robust manoeuvring abilities of flying animals, unmanned aerial systems are being developed and tested to improve flight control through cluttered environments. We previously examined steering strategies that pigeons adopt to fly through an array of vertical obstacles (VOs). Modelling VO flight guidance revealed that pigeons steer towards larger visual gaps when making fast steering decisions. In the present experiments, we recorded three-dimensional flight kinematics of pigeons as they flew through randomized arrays of horizontal obstacles (HOs)...
February 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/28064108/protection-of-male-reproductive-toxicity-in-rats-exposed-to-di-n-butyl-phthalate-during-embryonic-development-by-testosterone
#7
Nelli Giribabu, Pamanji Sreenivasula Reddy
Di-n-butyl phthalate (DBP) widely spread industrial chemical that made drastic alteration in male reproductive system. The present study elucidates the protective role of testosterone on reproductive toxicity in prenatal DBP exposed adult male rats. Pregnant rats were injected with corn oil or 100 and 500mg/kg body weight of DBP on gestation day (GD) 1, 7 and 14. F1 male rats were weaned, injected with either testosterone or vehicle. On postnatal day (PND) 100 F1 adult male rats were cohabited with untreated female rats...
March 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28058771/short-communication-progressive-motility-of-frozen-thawed-canine-semen-is-highest-five-minutes-after-thawing
#8
S Karger, B Geiser, M Grau, W Heuwieser, S P Arlt
Progressive motility is usually estimated by visual inspection using a light contrast microscope at X 100 immediately after semen collection or immediately after thawing frozen semen. Standard operating procedures have never been established for this test. The objective of this experiment was to examine time-dependent changes of motility after thawing cryopreserved canine semen. Semen of 35 dogs was collected, and volume, concentration, progressive motility, morphology, membrane integrity and HOS test were evaluated...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28017826/determining-vancomycin-etest-mics-in-patients-with-mrsa-bloodstream-infection-does-not-support-switching-antimicrobials
#9
Nina J Hos, Nathalie Jazmati, Danuta Stefanik, Martin Hellmich, Halil AlSael, Winfried V Kern, Siegbert Rieg, Hilmar Wisplinghoff, Harald Seifert, Achim J Kaasch
OBJECTIVES: Elevated vancomycin minimum inhibitory concentrations (MIC) have been reported to adversely affect clinical outcome in methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infection (BSI). We therefore examined the association between vancomycin MIC and outcome considering various potential confounders. METHODS: Clinical data and bacterial isolates were prospectively collected from patients with MRSA BSI from 2006 to 2012 as part of the Invasive Staphylococcus aureus Infection Cohort (INSTINCT) study...
March 2017: Journal of Infection
https://www.readbyqxmd.com/read/28011116/ethylene-glycol-but-not-dmso-could-replace-glycerol-inclusion-in-soybean-lecithin-based-extenders-in-ram-sperm-cryopreservation
#10
Abouzar Najafi, Hossein Daghigh-Kia, Hossein Vaseghi Dodaran, Mahdieh Mehdipour, Manuel Alvarez-Rodriguez
The aim of this study was to evaluate the effects of glycerol, ethylene glycol or DMSO in a soybean lecithin extender for freezing ram semen. In this study, 20 ejaculates were collected from four Ghezel rams and diluted with soybean lecithin extender with glycerol (7%), ethylene glycol (3%, 5% and 7%) or DMSO (3%, 5% and 7%). Sperm motility (CASA), membrane integrity (HOS test), viability, total abnormality, mitochondrial activity (Rhodamine 123) and apoptotic features (Annexin V/Propidium iodide) were assessed after thawing...
February 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/27999721/improvements-in-knee-extension-strength-are-associated-with-improvements-in-self-reported-hip-function-following-arthroscopy-for-femoroacetabular-impingement-syndrome
#11
Chelseana C Davis, Thomas J Ellis, Ajit K Amesur, Timothy E Hewett, Stephanie Di Stasi
BACKGROUND: Recovery of strength is critical for return to sport, and is a known predictor of functional outcomes in post-surgical orthopedic populations. Muscle weakness is a known impairment in patients with femoroacetabular impingement syndrome (FAIS) but whether improvements in muscle strength after arthroscopy are associated with improved hip function is unknown. HYPOTHESIS/PURPOSE: To examine the relationships between changes in hip and thigh muscle strength and self-reported function in athletes undergoing arthroscopy for FAIS...
December 2016: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/27999608/anticancer-activity-antioxidant-activity-and-phenolic-and-flavonoids-content-of-wild-tragopogon-porrifolius-plant-extracts
#12
Fuad Al-Rimawi, Suzi Rishmawi, Sharehan H Ariqat, Mahmoud F Khalid, Ismail Warad, Zaidoun Salah
Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer. Samples of Tragopogon porrifolius plant grown wild in Palestine were extracted with different solvents: water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AA). Four different antioxidant assays were used to evaluate AA of the extracts: two measures the reducing power of the extracts (ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant power (CUPRAC)), while two other assays measure the scavenging ability of the extracts (2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid (ABTS)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH))...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27860196/tricetin-inhibits-human-osteosarcoma-cells-metastasis-by-transcriptionally-repressing-mmp-9-via-p38-and-akt-pathways
#13
Pin-Yu Chang, Ming-Ju Hsieh, Yih-Shou Hsieh, Pei-Ni Chen, Jia-Sin Yang, Fang-Cheng Lo, Shun-Fa Yang, Ko-Hsiu Lu
Tricetin, a dietary flavonoid, has cytostatic properties and anti-metastasis activities in various cancer cells. However, the detailed impacts and underlying mechanisms of tricetin on human osteosarcoma cell metastasis are still unclear. Here, the hypothesis that tricetin possesses the anti-metastatic effects on human osteosarcoma cells was tested. The effects of tricetin on cell viability, motility, migration, and invasion in human osteosarcoma U2OS and HOS cells were investigated. Gelatin zymography, western blotting, polymerase chain reaction (PCR), and the luciferase assay were used to further explore the underlying mechanisms involved in anti-metastatic effects in U2OS cells...
November 8, 2016: Environmental Toxicology
https://www.readbyqxmd.com/read/27847489/how-do-maternal-subclinical-symptoms-influence-infant-motor-development-during-the-first-year-of-life
#14
Giulia Piallini, Stefania Brunoro, Chiara Fenocchio, Costanza Marini, Alessandra Simonelli, Marina Biancotto, Stefania Zoia
An unavoidable reciprocal influence characterizes the mother-child dyad. Within this relationship, the presence of depression, somatization, hostility, paranoid ideation, and interpersonal sensitivity symptoms at a subclinical level and their possible input on infant motor competences has not been yet considered. Bearing in mind that motor abilities represent not only an indicator of the infant's health-status, but also the principal field to infer his/her needs, feelings and intentions, in this study the quality of infants' movements were assessed and analyzed in relationship with the maternal attitudes...
2016: Frontiers in Psychology
https://www.readbyqxmd.com/read/27628727/an-automatic-prediction-of-epileptic-seizures-using-cloud-computing-and-wireless-sensor-networks
#15
Sanjay Sareen, Sandeep K Sood, Sunil Kumar Gupta
Epilepsy is one of the most common neurological disorders which is characterized by the spontaneous and unforeseeable occurrence of seizures. An automatic prediction of seizure can protect the patients from accidents and save their life. In this article, we proposed a mobile-based framework that automatically predict seizures using the information contained in electroencephalography (EEG) signals. The wireless sensor technology is used to capture the EEG signals of patients. The cloud-based services are used to collect and analyze the EEG data from the patient's mobile phone...
November 2016: Journal of Medical Systems
https://www.readbyqxmd.com/read/27579536/tumor-and-circulating-biomarkers-in-patients-with-second-line-hepatocellular-carcinoma-from-the-randomized-phase-ii-study-with-tivantinib
#16
Lorenza Rimassa, Giovanni Abbadessa, Nicola Personeni, Camillo Porta, Ivan Borbath, Bruno Daniele, Stefania Salvagni, Jean-Luc Van Laethem, Hans Van Vlierberghe, Jörg Trojan, Enrico N De Toni, Alan Weiss, Steven Miles, Antonio Gasbarrini, Monica Lencioni, Maria E Lamar, Yunxia Wang, Dale Shuster, Brian E Schwartz, Armando Santoro
ARQ 197-215 was a randomized placebo-controlled phase II study testing the MET inhibitor tivantinib in second-line hepatocellular carcinoma (HCC) patients. It identified tumor MET as a key biomarker in HCC.Aim of this research was to study the prognostic and predictive value of tumor (MET, the receptor tyrosine kinase encoded by the homonymous MNNG-HOS transforming gene) and circulating (MET, hepatocyte growth factor [HGF], alpha-fetoprotein [AFP], vascular endothelial growth factor [VEGF]) biomarkers in second-line HCC...
November 8, 2016: Oncotarget
https://www.readbyqxmd.com/read/27487911/suberanilohydroxamic-acid-vorinostat-synergistically-enhances-the-cytotoxicity-of-doxorubicin-and-cisplatin-in-osteosarcoma-cell-lines
#17
Aleksandra Pettke, Marc Hotfilder, Dagmar Clemens, Stephanie Klco-Brosius, Christiane Schaefer, Jenny Potratz, Uta Dirksen
Osteosarcoma is the most common primary bone cancer in children and is a highly malignant disease, in which 25% of patients present with metastasis at diagnosis. Considerable advances in the treatment of localized disease have been achieved since the introduction of combined modality treatment, increasing the prognosis of overall survival to 70%. Yet, established therapies have only limited success in treating both metastatic disease and nonresponders to primary chemotherapy. Therefore, new therapeutic approaches are required, particularly for the control of osteosarcoma in these patient groups...
November 2016: Anti-cancer Drugs
https://www.readbyqxmd.com/read/27416992/are-short-term-outcomes-of-hip-arthroscopy-in-patients-55-years-and-older-inferior-to-those-in-younger-patients
#18
Andrew J Bryan, Aaron J Krych, Ayoosh Pareek, Patrick J Reardon, Rebecca Berardelli, Bruce A Levy
BACKGROUND: Hip arthroscopy for young patients with femoroacetabular impingement (FAI) has been successful, but the efficacy of hip arthroscopy in older patients is not clearly defined. PURPOSE: To evaluate the clinical outcomes of patients 55 years and older who are undergoing hip arthroscopy and to compare outcomes with those of patients younger than 55 years. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: A total of 201 (63 male, 138 female) patients undergoing primary hip arthroscopy for FAI without radiographic arthritis (Tönnis grade <3) were isolated from a prospective database and stratified by age to <55-year and ≥55-year groups...
July 14, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27328482/sperm-with-an-abnormal-hypo-osmotic-swelling-test-normal-fertilization-normal-embryo-development-but-implantation-failure
#19
EDITORIAL
J H Check, J Aly
PURPOSE: To review the clinical importance of including the hypo-osmotic swelling (HOS) test in routine male fertility testing which in general is not evaluated by most physicians dealing with infertility. MATERIALS AND METHODS: Pregnancy rates were evaluated in patients with low HOS test scores. A low HOS test was specifically defined as having less than 50% of sperm exhibiting the normal physiologic response of tail swelling, when subjected to a hypo-osmolar solution...
2016: Clinical and Experimental Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27274424/pre-and-post-operative-self-reported-function-and-quality-of-life-in-women-with-and-without-generalized-joint-laxity-undergoing-hip-arthroscopy-for-femoroacetabular-impingement
#20
Mattie Pontiff, Matthew P Ithurburn, Thomas Ellis, Kathleen Cenkus, Stephanie Di Stasi
BACKGROUND: Generalized joint laxity is more prevalent in women than men and may lead to poorer post-operative outcomes in select orthopedic populations. There are no studies examining peri-operative function in patients with generalized joint laxity (GJL) and femoroacetabular impingement (FAI). PURPOSE: The purpose of this study was to determine the difference in perceived function and quality of life as measured by the Hip Outcome Score ADL subscale (HOS-ADL), International Hip Outcomes Tool (iHOT-33) and the Short Form 12-Item Health Survey (SF-12) in women with and without GJL prior to and six months after undergoing hip arthroscopy for FAI...
June 2016: International Journal of Sports Physical Therapy
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