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cutanous leishmaniasis treatment

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https://www.readbyqxmd.com/read/28222637/genotyping-leishmania-promastigotes-isolated-from-patients-with-cutaneous-leishmaniasis-in-south-eastern-turkey
#1
Fahriye Ekşi, Orhan Özgöztaşı, Tekin Karslıgil, Mustafa Sağlam
Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, which were genotyped with a restriction fragment length polymorphism (RFLP) analysis...
February 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28219639/leishmania-l-amazonensis-induces-hyperalgesia-in-balb-c-mice-contribution-of-endogenous-spinal-cord-tnf%C3%AE-and-nf%C3%AE%C2%BAb-activation
#2
Sergio M Borghi, Victor Fattori, Kenji W Ruiz-Miyazawa, Milena M Miranda-Sapla, Rúbia Casagrande, Phileno Pinge-Filho, Wander R Pavanelli, Waldiceu A Verri
: Cutaneous leishmaniasis (CL) is the most common form of the leishmaniasis in humans. Ulcerative painless skin lesions are predominant clinical features of CL. Wider data indicate pain accompanies human leishmaniasis, out with areas of painless ulcerative lesions per se. In rodents, Leishmania (L.) major infection induces nociceptive behaviors that correlate with peripheral cytokine levels. However, the role of the spinal cord in pain processing after Leishmania infection has not been investigated...
February 17, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28192528/cd8-t-cell-cytotoxicity-mediates-pathology-in-the-skin-by-inflammasome-activation-and-il-1%C3%AE-production
#3
Fernanda O Novais, Augusto M Carvalho, Megan L Clark, Lucas P Carvalho, Daniel P Beiting, Igor E Brodsky, Edgar M Carvalho, Phillip Scott
Deregulated CD8+ T cell cytotoxicity plays a central role in enhancing disease severity in several conditions. However, we have little understanding of the mechanisms by which immunopathology develops as a consequence of cytotoxicity. Using murine models of inflammation induced by the protozoan parasite leishmania, and data obtained from patients with cutaneous leishmaniasis, we uncovered a previously unrecognized role for NLRP3 inflammasome activation and IL-1β release as a detrimental consequence of CD8+ T cell-mediated cytotoxicity, ultimately resulting in chronic inflammation...
February 13, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28177049/n-acetyl-cysteine-inhibits-liver-oxidative-stress-markers-in-balb-c-mice-infected-with-leishmania-amazonensis
#4
Juciano Gasparotto, Alice Kunzler, Mario Roberto Senger, Celeste da Silva Freitas de Souza, Salvatore Giovanni de Simone, Rafael Calixto Bortolin, Nauana Somensi, Felipe Dal-Pizzol, José Claudio Fonseca Moreira, Ana Lúcia Abreu-Silva, Kátia da Silva Calabrese, Floriano Paes Silva, Daniel Pens Gelain
BACKGROUND: Leishmaniasis is a parasitosis caused by several species of the genus Leishmania. These parasites present high resistance against oxidative stress generated by inflammatory cells. OBJECTIVES: To investigate oxidative stress and molecular inflammatory markers in BALB/c mice infected with L. amazonensis and the effect of antioxidant treatment on these parameters. METHODS: Four months after infection, oxidative and inflammatory parameters of liver, kidneys, spleen, heart and lungs from BALB/c mice were assessed...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28167598/in-vitro-sensitivity-of-cutaneous-leishmania-promastigote-isolates-circulating-in-french-guiana-to-a-set-of-drugs
#5
Marine Ginouvès, Stéphane Simon, Mathieu Nacher, Magalie Demar, Bernard Carme, Pierre Couppié, Ghislaine Prévot
Anti-leishmaniasis drug resistance is a common problem worldwide. The aim of this study was to inventory the general in vitro level of sensitivity of Leishmania isolates circulating in French Guiana and to highlight potential in vitro pentamidine-resistant isolates. This sensitivity study was conducted on 36 patient-promastigote isolates for seven drugs (amphotericin B, azithromycin, fluconazole, meglumine antimoniate, miltefosine, paromomycin, and pentamidine) using the Cell Counting Kit-8 viability test. The IC50 values obtained were heterogeneous...
February 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28150041/evaluation-of-a-hypothetical-protein-for-serodiagnosis-and-as-a-potential-marker-for-post-treatment-serological-evaluation-of-tegumentary-leishmaniasis-patients
#6
Mariana Pedrosa Lima, Lourena Emanuele Costa, Mariana Costa Duarte, Daniel Menezes-Souza, Beatriz Cristina Silveira Salles, Thaís Teodoro de Oliveira Santos, Fernanda Fonseca Ramos, Miguel Angel Chávez-Fumagalli, Amanda Christine Silva Kursancew, Roberta Passamani Ambrósio, Bruno Mendes Roatt, Ricardo Andrez Machado-de-Ávila, Denise Utsch Gonçalves, Eduardo Antonio Ferraz Coelho
The serodiagnosis for tegumentary leishmaniasis (TL) presents problems related to the sensitivity and/or specificity of the tests. In the present study, an enzyme-linked immunosorbent assay (ELISA) technique was used to evaluate the performance from a Leishmania braziliensis hypothetical protein, LbHyM, in an attempt to compare its serological reactivity with a soluble Leishmania antigenic preparation (SLA) for the serodiagnosis of cutaneous (CL) and mucosal (ML) leishmaniasis. LbHyM was predicted to be a kinesin-like protein by bioinformatics tools...
February 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28137819/use-of-optical-imaging-technology-in-the-validation-of-a-new-rapid-cost-effective-drug-screen-as-part-of-a-tiered-in-vivo-screening-paradigm-for-development-of-drugs-to-treat-cutaneous-leishmaniasis
#7
Diana Caridha, Sandi Parriot, Thomas H Hudson, Thierry Lang, Franklyn Ngundam, Susan Leed, Jenell Sena, Michael Harris, Michael O'Neil, Richard Sciotti, Lisa Read, Herve Lecoeur, Mark Hickman, Max Grogl
In any drug discovery and development effort, reduction in the lead optimization cycle is critical to decrease the time to license and reduce cost. In addition, ethical guidelines call for more ethical use of animals to minimize the number of animals used and decrease their suffering. Therefore, any cutaneous Leishmaniasis drug development effort requires multiple tiers of in vivo testing that start with higher-throughput efficacy assessments and progress to lower-throughput models with the most clinical relevance...
January 30, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28131912/healing-leishmaniasis-in-amazonia-review-of-ethnomedicinal-concepts-and-pharmaco-chemical-analysis-of-traditional-treatments-to-inspire-modern-phytotherapies
#8
REVIEW
Guillaume Odonne, Emeline Houël, Geneviève Bourdy, Didier Stien
ETHNOPHARMACOLOGICAL RELEVANCE: Cutaneous/mucocutaneous leishmaniasis are neglected tropical diseases occurring in all intertropical regions of the world. Amazonian populations developed an abundant knowledge related to the disease and its remedies. Therefore, we undertook to review traditional antileishmanial plants in Amazonia, and developed new tools to analyze this somewhat dispersed information. MATERIAL AND METHODS: A review of the literature concerning traditional remedies against cutaneous/mucocutaneous leishmaniasis in the Amazon was conducted, and the obtained data used for the calculation of distribution indexes designed to highlight the most relevant uses in Amazonia...
January 25, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28127360/species-identification-and-molecular-typing-of-leishmania-spp-using-targeting-hsp70-gene-in-suspected-patients-of-cutaneous-leishmaniasis-from-sistan-and-baluchestan-province-southeast-iran
#9
Hadi Mirahmadi, Alireza Salimi Khorashad, Alireza Sohrabnahad, Peyman Heydarian, Negar Bizhani
BACKGROUND: Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed to detect the parasite species using the PCR-RFLP method. METHODS: A total of 130 Giemsa-Stained slides from suspected Cutaneous leishmaniasis (CL) patients were examined under a light microscope at ×1000...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28124961/-investigation-of-cutaneous-leishmaniasis-by-active-screening-in-primary-schools-in-sanliurfa-turkey
#10
Nebiye Yentür Doni, Gülcan Gürses, Reşat Dikme, Zeynep Şimşek, Mehmet Muratoğlu, Fadile Yıldız Zeyrek, Yavuz Yeşilova
Cutaneous leishmaniasis (CL) is endemic in Sanliurfa, a province located at southestern part of Turkey. The aims of this study were to determine CL cases among children aged 7-15 years attending primary schools and evaluate the demographical and clinical characteristics by active screening method. In our study, a total of 163.464 children attending to 123 primary schools have been evaluated in terms of CL prevalence between 2010 to 2012 period. Accordingly, 67.585, 72.279 and 23.600 students studying in 74, 37 and 12 primary schools were actively screened, respectively...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28122496/lip-leishmaniasis-a-case-series-with-molecular-identification-and-literature-review
#11
Iraj Mohammadpour, Mohammad Hossein Motazedian, Farhad Handjani, Gholam Reza Hatam
BACKGROUND: Mucocutaneous leishmaniasis (MCL), a protozoan infectious disease, is very rare in Iran despite the endemicity of both cutaneous and visceral forms. It is transmitted by the Phlebotomus sand fly. The lip is considered one of the extraordinary sites. Lesions usually initiate with erythematous papules, slowly enlarges and then it ulcerates. The diagnosis of MCL encompasses epidemiological, clinical and laboratory aspects. Usually, the combination of some of these elements is necessary for the final diagnosis...
January 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28106921/evaluation-of-efficacy-of-impregnated-curtains-in-experimental-hen-houses-as-a-phlebotomine-control-tool-in-northeast-argentina
#12
M Manteca Acosta, M S Santini, A A Pérez, O D Salomón
The aim of this study was to evaluate the effectiveness of insecticide-impregnated curtains against the entry of phlebotomine (Diptera: Psychodidae) flies into experimental slatted hen houses in an area endemic for American cutaneous leishmaniasis (ACL). Three treatments in experimental dwellings containing three chickens each were applied using, respectively, an impregnated curtain (IC), a non-impregnated curtain (NIC) and no curtain (NC). A control site without chickens (WC) was included. The study used permethrin at 0...
January 20, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28103966/chemotherapy-of-leishmaniasis-present-challenges
#13
Silvia R B Uliana, Cristiana T Trinconi, Adriano C Coelho
Cutaneous and visceral leishmaniasis are amongst the most devastating infectious diseases of our time, affecting millions of people worldwide. The treatment of these serious diseases rely on a few chemotherapeutic agents, most of which are of parenteral use and induce severe side-effects. Furthermore, rates of treatment failure are high and have been linked to drug resistance in some areas. Here, we reviewed data on current chemotherapy practice in leishmaniasis. Drug resistance and mechanisms of resistance are described as well as the prospects for applying drug combinations for leishmaniasis chemotherapy...
January 20, 2017: Parasitology
https://www.readbyqxmd.com/read/28096850/expression-analysis-of-multiple-genes-may-involve-in-antimony-resistance-among-leishmania-major-clinical-isolates-from-fars-province-central-iran
#14
Nafiseh Ghobakhloo, Mohammad Hossein Motazedian, Majid Fardaei
BACKGROUND: Treatment of Cutaneous Leishmaniasis (CL) is being faced with serious difficulties in Fars Province, due to emerging of resistance against meglumine antimonite (Glucantime®). In this context, determining some biomarkers for drug sensitivity monitoring seems to be highly essential. Different studies have been carried out to decipher the genes might be involved in antimony resistant phenotype in Leishmania spp. Here, we selected three genes: AQP (as drug transporter), TDR-1-1(as drug activator), and γ-GCS (inducing reduction environment) for comparative expression analysis on clinical resistant and sensitive isolates of L...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28071592/development-and-evaluation-of-zinc-phthalocyanine-nanoemulsions-for-use-in-photodynamic-therapy-for-leishmania-spp
#15
Luciana Betzler de Oliveira de Siqueira, Verônica da Silva Cardoso, Igor Almeida Rodrigues, Ana Lúcia Vazquez-Villa, Elisabete Pereira Dos Santos, Bruno da Costa Leal Ribeiro Guimarães, Cristal Dos Santos Cerqueira Coutinho, Alane Beatriz Vermelho, Eduardo Ricci Junior
Photodynamic therapy (PDT) combines light with photosensitizers (PS) for production of reactive oxygen species (ROS) that can kill infectious microorganisms such as bacteria, fungi and protozoa. The application of nanotechnology has enabled the advancement of PDT because many PS are insoluble in water, necessitating a nanocarrier as a physiologically acceptable carrier. Nanoemulsions are efficient nanocarriers for solubilizing liposoluble drugs, like the PS, in water. Cutaneous (CL) and mucocutaneous leishmaniasis (ML) are caused by different species of the genus Leishmania, transmitted to humans by sandfly bites...
February 10, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28052994/local-delivery-of-the-tlr9-ligand-cpg-down-regulates-host-immune-and-inflammatory-responses-ameliorating-established-leishmania-viannia-panamensis-chronic-infection
#16
Allison K Ehrlich, Olga L Fernández, Daniel Rodriguez-Pinto, Tiago M Castilho, Maria J Corral Caridad, Karen Goldsmith-Pestana, Nancy Gore Saravia, Diane McMahon-Pratt
Infection by Leishmania (Viannia) panamensis, the predominant etiologic agent for cutaneous leishmaniasis in Colombia, is characterized by a chronic mixed-inflammatory response. Current treatment options are plagued by toxicity, lengthy treatment regimens and growing evidence of drug resistance. Immunotherapy, modulating the immune system to mount a protective response, may provide an alternate therapeutic approach. We investigated the ability of the TLR9 ligand, CpG, to modulate established disease in the L...
January 4, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28043177/the-pulsed-dye-laser-is-more-effective-and-rapidly-acting-than-intralesional-meglumine-antimoniate-therapy-for-cutaneous-leishmaniasis
#17
Mohammad Radmanesh, Ehsan Omidian
BACKGROUND AND OBJECTIVE: The Pulsed dye laser (PDL) is not tested yet for the treatment of cutaneous leishmaniasis (CL). We compared the efficacy of 585nm PDL with intralesional meglumine antimoniate (MA) on 17 patients with 81 lesions of cutaneous leishmaniasis. PATIENTS AND METHOD: Out of the 81 lesions, 42 lesions were treated with one or two passes of PDL fortnightly and 39 lesions in the same patients were treated with intralesional MA weekly. The fluence chosen for the laser was 7 Joules/cm2 with 5mm spot size...
January 3, 2017: Journal of Dermatological Treatment
https://www.readbyqxmd.com/read/28011169/leishmanicidal-activity-of-the-alkaloid-rich-fraction-from-guatteria-latifolia
#18
C Ferreira, C L A Passos, D C Soares, K P Costa, M J C Rezende, A Q Lobão, A C Pinto, L Hamerski, E M Saraiva
Leishmaniasis is caused by protozoan parasites belonging to the genus Leishmania and includes cutaneous, mucocutaneous and visceral clinical forms. The drugs currently available for leishmaniasis treatment are pentavalent antimonials, amphotericin B and miltefosine, which present high toxicity, elevated cost and development of parasite resistance. The natural products constitute an important source of substances with leishmanicidal potential. Here we evaluated in vitro the anti-Leishmania amazonensis activity of crude extracts of branches, leaves and fruits of Guatteria latifolia...
January 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28008252/nanoscaled-hydrated-antimony-v-oxide-as-a-new-approach-to-first-line-antileishmanial-drugs
#19
Antonia Mr Franco, Iryna Grafova, Fabiane V Soares, Gennaro Gentile, Claudia Dc Wyrepkowski, Marcos A Bolson, Ézio Sargentini, Cosimo Carfagna, Markku Leskelä, Andriy Grafov
BACKGROUND: Coordination compounds of pentavalent antimony have been, and remain, the first-line drugs in leishmaniasis treatment for >70 years. Molecular forms of Sb (V) complexes are commercialized as sodium stibogluconate (Pentostam(®)) and meglumine antimoniate (MA) (Glucantime(®)). Ever-increasing drug resistance in the parasites limits the use of antimonials, due to the low drug concentrations being administered against high parasitic counts. Sb(5+) toxicity provokes severe side effects during treatment...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28001228/standardization-of-intralesional-meglumine-antimoniate-treatment-for-cutaneous-leishmaniasis
#20
Maria Cristina de Oliveira Duque, Érica de Camargo Ferreira E Vasconcellos, Maria Inês Fernandes Pimentel, Marcelo Rosandiski Lyra, Sandro Javier Bedoya Pacheco, Mauro Celio de Almeida Marzochi, Cláudia Maria Valete Rosalino, Armando de Oliveira Schubach
INTRODUCTION: Intralesional treatment for cutaneous leishmaniasis has been applied for over 30 years at the Oswaldo Cruz Foundation, Rio de Janeiro, with good therapeutic results and without relevant systemic toxicity. METHODS: Meglumine antimoniate was injected subcutaneously, using a long medium-caliber needle (for example, 30mm × 0.8mm); patients received 1-3 injections, with 15-day intervals. RESULTS: The technique is described in detail sufficient to enable replication...
November 2016: Revista da Sociedade Brasileira de Medicina Tropical
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