keyword
MENU ▼
Read by QxMD icon Read
search

Dialyzer

keyword
https://www.readbyqxmd.com/read/29351065/measurement-of-binding-strength-between-prey-proteins-interacting-with-toxoplasma-gondii-sag1-and-sag2-using-isothermal-titration-calorimetry-itc
#1
Meng-Yee Lai, Yee-Ling Lau
Following the outcome from a previously performed yeast two-hybrid experiment, the binding strength between T. gondii SAG1 and SAG2 and their respective prey proteins were further confirmed in this study. The sag1, sag2 and their prey genes were amplified and cloned into a pGEMT vector. To express the recombinant proteins, the fragments were then subcloned into a pRSETA vector and transformed into E. coli BL21 (DE3) cells. The recombinant proteins were expressed optimally at 37°C and 1mM of IPTG. The 6X His-tag fusion proteins were purified, dialyzed and concentrated...
March 26, 2018: Acta Parasitologica
https://www.readbyqxmd.com/read/29350348/snf472-a-novel-inhibitor-of-vascular-calcification-could-be-administered-during-hemodialysis-to-attain-potentially-therapeutic-phytate-levels
#2
Joan Perelló, M Gómez, M D Ferrer, N Y Rodríguez, C Salcedo, J M Buades, M M Pérez, J V Torregrosa, E Martín, F Maduell
BACKGROUND: Cardiovascular calcification (CVC) is a major concern in hemodialysis (HD) and the loss of endogenous modulators of calcification seems involved in the process. Phytate is an endogenous crystallization inhibitor and its low molecular mass and high water solubility make it potentially dialyzable. SNF472 (the hexasodium salt of phytate) is being developed for the treatment of calciphylaxis and CVC in HD patients. We aimed to verify if phytate is lost during dialysis, and evaluate SNF472's behaviour during dialysis...
January 19, 2018: Journal of Nephrology
https://www.readbyqxmd.com/read/29341167/the-influence-of-colloid-osmotic-pressure-on-hydrostatic-pressures-in-high-and-low-flux-hemodialyzers
#3
Daniel Schneditz, Georgios Sarikakis, Maria Kontodima, Notburga Sauseng
The aim of this study was to examine the relationship between hydrostatic trans-membrane pressure (TMPh ) and colloid osmotic pressure (COP) in low-flux (LF) and high-flux (HF) dialyzers. Hydrostatic pressures were measured in dialyzers distinguished by their ultrafiltration coefficient Kuf (16 and 85 mL/h/mm Hg) under constant dialysate flow and variable blood flow (Qb ) ranging from 0 to 400 mL/min using (i) alginate (70 kDa) dissolved in dialysate, (ii) diluted, undiluted, and concentrated plasma, or (iii) whole blood at different hematocrit, all in absence of ultrafiltration (UF)...
January 17, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29327645/identification-and-characterization-of-chitinolytic-bacteria-isolated-from-a-freshwater-lake
#4
Dinh Minh Tran, Hayuki Sugimoto, Dzung Anh Nguyen, Takeshi Watanabe, Kazushi Suzuki
To develop a novel type of biocontrol agent, we focus on bacteria that are characterized by both chitinase activity and biofilm development. Chitinolytic bacteria were isolated from sediments and chitin flakes immersed in the water of a sand dune lake, Sakata, in Niigata, Japan. Thirty-one isolates from more than 5100 isolated strains were examined chitinase activity and biofilm formation. Phylogenetic analysis of these isolates based on the 16S rRNA gene sequences revealed that most isolates belonged to the family Aeromonadaceae, followed by Paenibacillaceae, Enterobacteriaceae, and Neisseriaceae...
January 12, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29316531/graphene-oxide-doping-improves-the-biocompatibility-and-separation-performance-of-polyethersulfone-hollow-fiber-membranes-for-bioartificial-kidney-application
#5
Akshay Modi, Surendra Kumar Verma, Jayesh Bellare
HYPOTHESIS: Graphene oxide (GO)-doping in polyethersulfone hollow fiber membranes (PES HFMs) improves the biocompatibility and separation performance for bioartificial kidney (BAK) application. EXPERIMENTS: GO was doped in PES HFMs. The physicochemical characterization of the developed HFMs was carried out. The biocompatibility tests including hemocompatibility and cytotoxicity tests, and separation experiments including uremic toxins clearance were performed. FINDINGS: GO-doping resulted in low hemolysis (0...
December 27, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29314087/ultrasonographic-assessment-of-the-internal-jugular-vein-for-the-estimation-of-central-venous-pressure-in-hemodialysis-patients-a-preliminary-study
#6
Basile Kerleroux, Jeremy Pasco, Mathieu Dupuis, Marine Eustache, Ahmed Lemrabott, Charlotte Jouzel, Catherine Albert, Kevin Janot, Baptiste Morel, André Pruna
PURPOSE: To investigate a new noninvasive method to assess central venous pressure (CVP) in hemodialysis patients, based on the ultrasonographic measurement of the collapsing point of the internal jugular vein (CVPni). MATERIALS AND METHODS: In this preliminary, noninterventional, single center study, we enrolled 22 dialyzed patients with an indwelling jugular catheter. CVPni was compared to the gold-standard invasive measurement of CVP using the central venous catheter (CVPi)...
January 4, 2018: Journal of Clinical Ultrasound: JCU
https://www.readbyqxmd.com/read/29304162/adrenergic-receptors-inhibit-trpv1-activity-in-the-dorsal-root-ganglion-neurons-of-rats
#7
Yumi Matsushita, Miki Manabe, Naoki Kitamura, Izumi Shibuya
Transient receptor potential vanilloid type 1 (TRPV1) is a polymodal receptor channel that responds to multiple types of stimuli, such as heat, acid, mechanical pressure and some vanilloids. Capsaicin is the most commonly used vanilloid to stimulate TRPV1. TRPV1 channels are expressed in dorsal root ganglion neurons that extend to Aδ- and C-fibers and have a role in the transduction of noxious inputs to the skin into the electrical signals of the sensory nerve. Although noradrenergic nervous systems, including the descending antinociceptive system and the sympathetic nervous system, are known to modulate pain sensation, the functional association between TRPV1 and noradrenaline in primary sensory neurons has rarely been examined...
2018: PloS One
https://www.readbyqxmd.com/read/29298674/immunepotent-crp-induces-cell-cycle-arrest-and-caspase-independent-regulated-cell-death-in-hela-cells-through-reactive-oxygen-species-production
#8
Ana Carolina Martínez-Torres, Alejandra Reyes-Ruiz, Milena Benítez-Londoño, Moises Armides Franco-Molina, Cristina Rodríguez-Padilla
BACKGROUND: Regulated cell death (RCD) is a mechanism by which the cell activates its own machinery to self-destruct. RCD is important for the maintenance of tissue homeostasis and its deregulation is involved in diseases such as cervical cancer. IMMUNEPOTENT CRP (I-CRP) is a dialyzable bovine leukocyte extract that contains transfer factors and acts as an immunomodulator, and can be cytotoxic to cancer cell lines and reduce tumor burden in vivo. Although I-CRP has shown to improve or modulate immune response in inflammation, infectious diseases and cancer, its widespread use has been limited by the absence of conclusive data on the molecular mechanism of its action...
January 3, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29286371/preparation-of-keratin-hydrolysate-from-chicken-feathers-and-its-application-in-cosmetics
#9
Pavel Mokrejš, Matouš Huťťa, Jana Pavlačková, Pavlína Egner
Keratin hydrolysates (KHs) are established standard components in hair cosmetics. Understanding the moisturizing effects of KH is advantageous for skin-care cosmetics. The goals of the protocol are: (1) to process chicken feathers into KH by alkaline-enzymatic hydrolysis and purify it by dialysis, and (2) to test if adding KH into an ointment base (OB) increases hydration of the skin and improves skin barrier function by diminishing transepidermal water loss (TEWL). During alkaline-enzymatic hydrolysis feathers are first incubated at a higher temperature in an alkaline environment and then, under mild conditions, hydrolyzed with proteolytic enzyme...
November 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29273022/on-line-hemodiafiltration-did-not-induce-an-overproduction-of-oxidative-stress-and-inflammatory-cytokines-in-intensive-care-unit-acute-kidney-injury
#10
Kada Klouche, Laurent Amigues, Marion Morena, Vincent Brunot, Anne Marie Dupuy, Audrey Jaussent, Marie Christine Picot, Noémie Besnard, Delphine Daubin, Jean Paul Cristol
BACKGROUND: Though on-line intermittent hemodiafiltration (OL-IHDF) is a routine therapy for chronic dialysis patients, it is not yet widespread used in critically ill patients. This study was undergone to evaluate efficiency and tolerance of OL-IHDF and to appreciate inflammatory consequences of its use in intensive care unit (ICU)-acute kidney injury (AKI) patients. METHODS: In this prospective cohort study conducted in a medical academic ICU in France, 30 AKI patients who underwent OL-IHDF were included...
December 22, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/29248767/efficient-purification-of-apolipoprotein-a1-apoa1-from-plasma-by-hea-hypercel%C3%A2-an-alternative-approach
#11
Arun Govind G, Agamudi Shivasankaran Kamalanathan, Mookambeswaran Arunachalam Vijayalakshmi, Krishnan Venkataraman
HDL-ApoA1 plays a pivotal role in the prevention of atherosclerosis and cardiovascular diseases. ApoA1 purification from blood plasma has always remained tedious, involving multiple steps, large volumes of plasma and substantial loss in the final yield of pure ApoA1. In this study, a two-step method has been developed and optimized for the purification of ApoA1 from plasma. Plasma was first subjected to 60% ammonium sulphate (NH4)2SO4 precipitation and subsequently, ApoA1 was recovered using mixed mode chromatographic sorbent, HEA HyperCel™...
December 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29244856/in-vitro-anthelmintic-effects-of-spigelia-anthelmia-protein-fractions-against-haemonchus-contortus
#12
Sandra Alves Araújo, Alexandra Martins Dos Santos Soares, Carolina Rocha Silva, Eduardo Bezerra Almeida Júnior, Cláudia Quintino Rocha, André Teixeira da Silva Ferreira, Jonas Perales, Livio M Costa-Júnior
Gastrointestinal nematodes are a significant concern for animal health and well-being, and anthelmintic treatment is mainly performed through the use of chemical products. However, bioactive compounds produced by plants have shown promise for development as novel anthelmintics. The aim of this study is to assess the anthelmintic activity of protein fractions from Spigelia anthelmia on the gastrointestinal nematode Haemonchus contortus. Plant parts were separated into leaves, stems and roots, washed with distilled water, freeze-dried and ground into a fine powder...
2017: PloS One
https://www.readbyqxmd.com/read/29240855/lawrence-transfer-factor-transference-of-specifc-immune-memory-by-dialyzable-leukocyte-extract-from-a-cd8-t-cell-line
#13
Jason F Wang, Andrew J Park, Tina Rendini, William R Levis
<p>Lawrence transfer factor (TF) is defned as dialyzable leukocyte extract (DLE) that can transfer antigen-specifc cell-mediated immunity from a person testing positive for the antigen in a delayed type hypersensitivity skin test manner to a person negative for the same antigen.</p> <p>A recent article by Myles et al1 has identifed a DLE isolated from an established CD8+ T cell line capable of transferring antigen-specifc immunity. The DLE contains a portion of the beta chain of the T cell receptor and additional nucleotide and protein factors that are being subjected to further modern biochemical analysis...
December 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29232732/high-salt-intake-promotes-different-responses-to-urodilatin-and-uroguanylin-in-the-isolated-rat-kidney
#14
Antonio Rafael Coelho Jorge, Pedro Henrique Sá Costa, Helena Serra Azul Monteiro, Manassés Claudino Fonteles
Urodilatin (UD) and uroguanylin (UGN) have been implicated in the regulation of salt and water homeostasis, particularly in the balance handling of salt intake. In this sense, the aim of the present work was to study the main effects of these peptides in kidneys from animals subjected to high NaCl (2%) intake, during 10 days in metabolic cages. The control group received only normal water, whereas the treated group drank 2% solution of NaCl (NaCl 2%). In addition, we studied effect of subthreshold UD (0.14 nM) and UGN (0...
December 12, 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/29230564/adsorptive-filtration-systems-for-effective-removal-of-blood-amyloid-%C3%AE-a-potential-therapy-for-alzheimer-s-disease
#15
Nobuya Kitaguchi, Kazunori Kawaguchi, Kazunori Yamazaki, Hiroshi Kawachi, Miwa Sakata, Megumi Kaneko, Masao Kato, Kazuyoshi Sakai, Norimi Ohashi, Midori Hasegawa, Yoshiyuki Hiki, Yukio Yuzawa
Accumulation of amyloid-β protein (Aβ) in the brain causes cognitive impairment in Alzheimer's disease. We hypothesized that an extracorporeal system that rapidly removed Aβ from the blood may accelerate Aβ drainage from the brain. We previously reported that dialyzers remove blood Aβs effectively, mainly by adsorption on the inner surfaces of the hollow fibers, resulting in lower Aβ accumulation in the brains of patients undergoing hemodialysis than the controls without hemodialysis. The aim of the present study was to create a more convenient and effective blood Aβ removal system using adsorptive filtration, in which the filtrate returned to the body...
December 11, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/29227979/higher-serum-hepatocyte-growth-factor-concentration-is-associated-with-better-preservation-of-gfr-in-hemodialysis-patients
#16
Ewa Baum, Beata Maćkowiak, Patrycja Sosińska-Zawierucha, Krzysztof Pawlaczyk, Barbara Kołodziejczak, Andrzej Breborowicz
BACKGROUND/AIMS: Hemodialysis induces an intravascular inflammatory reaction which may further deteriorate renal function. We studied changes of serum interleukin 6 (IL6) and hepatocyte growth factor (HGF) concentrations during dialysis sessions, and at 12 month intervals. The synthesis of these cytokines in arterial endothelial cells in the presence of serum obtained from dialyzed patients was studied. Changes of the inflammatory reaction during 12 months of treatment were correlated with GFR...
December 8, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29223661/-is-cancer-incidence-different-between-type-2-diabetes-patients-compared-to-non-diabetics-in-hemodialysis-a-study-from-the-rein-registry
#17
Aurélie Le Guillou, Adelaide Pladys, Wahida Kihal, Muriel Siebert, Atman Haddj-Elmrabet, Charlotte Cernon, Anne Bernard, Christophe Charasse, Lise Mandart, Didier Hamel, Tugdual Tanquerel, Bernard Strullu, Christine Richer, Pascale Siohan, Théophile Sawadogo, Juliette Baleynaud, Simona Baluta, Sahar Bayat, Cécile Vigneau
BACKGROUND AND OBJECTIVES: In France, diabetes mellitus is now the second cause of end stage renal disease. In a large previous French national study, we observed that dialyzed diabetics have a significant lower risk of death by cancer. This first study was focused on cancer death but did not investigate cancer incidence. In this context, the aim of this second study was to compare the incidence of cancer in diabetic dialyzed patients compared to non-diabetic dialyzed patients in a French region...
December 6, 2017: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29222018/physicochemical-properties-and-biological-activities-of-polysaccharides-from-lycium-barbarum-prepared-by-fractional-precipitation
#18
Guiping Gong, Tiantian Dang, Yangni Deng, Jianli Han, Sun Jing, Ying Zhang, Linjuan Huang, Zhongfu Wang
Traditional separation and purification process of Lycium barbarum polysaccharide (LBP) includes water extraction, alcohol precipitation, deproteinization and ion-exchange column chromatography, which is complicated and time-consuming. In our study, non-dialyzable LBP-I and dialyzable LBP-O were obtained from LBP by water extraction, alcohol precipitation and deproteinization. LBP-I was separated by fractional precipitation and three fractions (LBP-I-1, LBP-I-2 and LBP-I-3) were obtained. The three fractions were further purified by gel permeation chromatography to LBGP-I-1, LBGP-I-2 and LBGP-I-3 with yields of 0...
December 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29220160/plant-protein-directed-synthesis-of-luminescent-gold-nanoclusters-hybrids-for-tumor-imaging
#19
Zhao Li, Haibao Peng, Jialin Liu, Ye Tian, Wuli Yang, Jinrong Yao, Zhengzhong Shao, Xin Chen
Nowadays, fluorescence detection has emerged as one of the most frequently used non-invasive biosensing method to selectively monitor biological processes within living systems. Among fluorescent nanoparticles, gold nanoclusters (AuNCs) have been intensively studied because of their intrinsic fluorescence and their endowed biocompatible surface. Herein, we selected an abundant, low-cost and sustainable plant protein, the pea protein isolate (PPI) for its excellent biocompatibility, biodegradability, and non-allergenic character, to be employed as both a reducing and stabilizing agent to facilely produce AuNCs exhibiting a strong red fluorescence...
December 8, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29209721/effect-of-high-cutoff-hemodialysis-vs-conventional-hemodialysis-on-hemodialysis-independence-among-patients-with-myeloma-cast-nephropathy-a-randomized-clinical-trial
#20
RANDOMIZED CONTROLLED TRIAL
Frank Bridoux, Pierre-Louis Carron, Brigitte Pegourie, Eric Alamartine, Karine Augeul-Meunier, Alexandre Karras, Bertrand Joly, Marie-Noëlle Peraldi, Bertrand Arnulf, Cécile Vigneau, Thierry Lamy, Alain Wynckel, Brigitte Kolb, Bruno Royer, Nolwenn Rabot, Lotfi Benboubker, Christian Combe, Arnaud Jaccard, Bruno Moulin, Bertrand Knebelmann, Sylvie Chevret, Jean-Paul Fermand
Importance: Cast nephropathy is the main cause of acute kidney injury in multiple myeloma and persistent reduction in kidney function strongly affects prognosis. Strategies to rapidly remove nephrotoxic serum-free light chains combined with novel antimyeloma agents have not been evaluated prospectively. Objective: To compare the hemodialysis independence rate among patients newly diagnosed with myeloma cast nephropathy treated with hemodialysis using a high-cutoff dialyzer (with very large membrane pores and high permeability to immunoglobulin light chains) or a conventional high-flux dialyzer (with small pores and lower permeability)...
December 5, 2017: JAMA: the Journal of the American Medical Association
keyword
keyword
9446
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"