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https://www.readbyqxmd.com/read/29222948/force-coordination-strategies-in-on-water-single-sculling-are-asymmetries-related-to-better-rowing-performance
#1
John Warmenhoven, Richard Smith, Conny Draper, Andrew Harrison, Norma Bargary, Stephen Cobley
Asymmetries of the rowing stroke cycle have been assessed with reference to kinematics and foot-force measures in laboratory testing environments. It remains unclear how asymmetries in propulsive kinetic measures are related to on-water rowing performance. A new approach for the evaluation of both global and local asymmetries across the entire movement was used to assess the continuous role of asymmetries and whether these change according to level of competitive representation. Twenty seven highly skilled female rowers (national and international competition level), rowing at 32 strokes per minute in a single scull boat were evaluated...
December 9, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29220331/regenerative-potential-of-secretome-from-dental-stem-cells-a-systematic-review-of-preclinical-studies
#2
Suleiman Alhaji Muhammad, Norshariza Nordin, Sharida Fakurazi
Injury to tissues is a major clinical challenge due to the limited regenerative capacity of endogenous cells. Stem cell therapy is evolving rapidly as an alternative for tissue regeneration. However, increasing evidence suggests that the regenerative ability of stem cells is mainly mediated by paracrine actions of secretome that are generally secreted by the cells. We aimed to systematically evaluate the efficacy of dental stem cell (DSC)-conditioned medium in in vivo animal models of various tissue defects...
December 8, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/29217940/audit-of-oral-histopathology-service-at-a-nigerian-tertiary-institution-over-a-24-year-period
#3
Akindayo O Akinyamoju, Bukola F Adeyemi, Akinyele O Adisa, Chidinma N Okoli
Background: Biopsies are often essential for definitive diagnosis of oro-facial lesions and are a part of oral diagnostic procedures carried out in histopathology laboratories. At present, there is paucity of literature on the audit of oral histopathology services in Nigeria. The objectives of this study were to determine the prevalence of biopsied oral lesions in a Nigerian tertiary institution. Also to profile the usage of oral pathology service and to identify challenges that may be present in an oral histodiagnostic service...
July 2017: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/29216879/anti-diabetic-potential-of-sapium-ellipticum-hochst-pax-leaf-extract-in-streptozotocin-stz-induced-diabetic-wistar-rats
#4
Osasenaga Macdonald Ighodaro, Oluseyi Adeboye Akinloye
BACKGOUND: Ethnobatanical survey associates Sapium ellipticum (SE) with antidiabetic usage among other medicinal functions in different parts of Africa. More importantly, previous studies on the plant extract in our laboratory showed that SE has significant effects on the activities of carbohydrate metabolizing enzymes such as glucokinase, glucose-6-phosphatase, α-amylase and α-glucosidase. In view of these, the anti-diabetic potential of the plant leaf extract in streptozotocin (STZ)-induced diabetes rat model (Wistar strain) was examined...
December 8, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29214754/utility-of-conventional-culture-and-maldi-tof-ms-for-identification-of-microbial-communities-in-bronchoalveolar-lavage-fluid-in-comparison-with-the-gs-junior-next-generation-sequencing-system
#5
Ji Yeon Sung, Younjee Hwang, Mi Hwa Shin, Moo Suk Park, Sang Hoon Lee, Dongeun Yong, Kyungwon Lee
BACKGROUND: Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical microbiology laboratories. Therefore, we evaluated the performance of conventional culture methods together with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying microbiota in bronchoalveolar lavage (BAL) fluid. METHODS: BAL fluid samples (n=27) were obtained from patients undergoing diagnostic bronchoscopy for lung mass evaluation...
March 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29214534/development-of-a-sandwich-elisa-with-potential-for-selective-quantification-of-human-lactoferrin-protein-nitrated-through-disease-or-environmental-exposure
#6
Amani Y Alhalwani, John E Repine, Michelle K Knowles, J Alex Huffman
Lactoferrin (LF) is an important multifunctional protein that comprises a large fraction of the protein mass in certain human fluids and tissues, and its concentration is often used to assess health and disease. LF can be nitrated by multiple routes, leading to changes in protein structure, and nitrated proteins can negatively impact physiological health via nitrosative stress. Despite an awareness of the detrimental effects of nitrated proteins and the importance of LF within the body, cost-effective methods for detecting and quantifying nitrated lactoferrin (NLF) are lacking...
December 6, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29214372/a-review-of-the-clinical-utility-of-systematic-behavioral-observations-in-attention-deficit-hyperactivity-disorder-adhd
#7
Franziska Minder, Agnieszka Zuberer, Daniel Brandeis, Renate Drechsler
This review evaluates the clinical utility of tools for systematic behavioral observation in different settings for children and adolescents with ADHD. A comprehensive search yielded 135 relevant results since 1990. Observations from naturalistic settings were grouped into observations of classroom behavior (n = 58) and of social interactions (n = 25). Laboratory observations were subdivided into four contexts: independent play (n = 9), test session (n = 27), parent interaction (n = 11), and peer interaction (n = 5)...
December 6, 2017: Child Psychiatry and Human Development
https://www.readbyqxmd.com/read/29213313/agronomic-performance-of-populus-deltoides-trees-engineered-for-biofuel-production
#8
David Macaya-Sanz, Jin-Gui Chen, Udaya C Kalluri, Wellington Muchero, Timothy J Tschaplinski, Lee E Gunter, Sandra J Simon, Ajaya K Biswal, Anthony C Bryan, Raja Payyavula, Meng Xie, Yongil Yang, Jin Zhang, Debra Mohnen, Gerald A Tuskan, Stephen P DiFazio
Background: One of the major barriers to the development of lignocellulosic feedstocks is the recalcitrance of plant cell walls to deconstruction and saccharification. Recalcitrance can be reduced by targeting genes involved in cell wall biosynthesis, but this can have unintended consequences that compromise the agronomic performance of the trees under field conditions. Here we report the results of a field trial of fourteen distinct transgenic Populus deltoides lines that had previously demonstrated reduced recalcitrance without yield penalties under greenhouse conditions...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29212699/rapid-microarray-based-detection-of-rifampicin-isoniazid-and-fluoroquinolone-resistance-in-mycobacterium-tuberculosis-using-a-single-cartridge
#9
Juliane Havlicek, Beatrice Dachsel, Peter Slickers, Sönke Andres, Patrick Beckert, Silke Feuerriegel, Stefan Niemann, Matthias Merker, Ines Labugger
Rapid and robust identification of mutations in Mycobacterium tuberculosis complex (MTBC) strains mediating multidrug and extensively drug-resistant (M/XDR) phenotypes is crucial to combat the MDR tuberculosis (TB) epidemic. Currently available molecular TB drug susceptibility tests are either restricted to single targets/drugs (i.e. Xpert MTB/RIF) or aid the risk of cross-contaminations due to the design limitations of the open platform (i.e. line probe assays).With a good understanding of the technical and commercial boundaries we designed a test cartridge with an introduction of dried reagents, based on an oligonucleotide array, which has the ability to identify MTBC strains resistant to isoniazid, rifampicin and the fluoroquinolones...
December 6, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29212517/is-hematoxylin-eosin-staining-in-rectal-mucosal-and-submucosal-biopsies-still-useful-for-the-diagnosis-of-hirschsprung-disease
#10
Suellen Serafini, Maria Mercês Santos, Ana Cristina Aoun Tannuri, Maria Claudia Nogueira Zerbini, Maria Cecília de Mendonça Coelho, Josiane de Oliveira Gonçalves, Uenis Tannuri
BACKGROUND: Hematoxylin-eosin (HE) staining of a full-thickness rectal wall fragment is classically used for the diagnosis of Hirschsprung disease (HD). However, this technique requires large fragments for a better diagnosis. Additionally, the histochemical and immunohistochemical methods of staining small fragments of rectal mucosal and submucosal biopsies are not available in all centers. Therefore, the possibility of diagnosing HD through HE staining in these biopsies could be a valuable alternative for centers that do not have more specific techniques...
December 6, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/29211969/use-of-platelet-rich-plasma-immediately-after-an-injury-did-not-improve-ligament-healing-and-increasing-platelet-concentrations-was-detrimental-in-an-in-vivo-animal-model
#11
Robert F LaPrade, Laurie R Goodrich, Jennifer Phillips, Grant J Dornan, Travis Lee Turnbull, Michael L Hawes, Kimi D Dahl, Ashley N Coggins, John Kisiday, David Frisbie, Jorge Chahla
BACKGROUND: Limited information in basic science and clinical trials exists to determine if ligament healing may be accelerated with the use of biological adjuvants, such as platelet-rich plasma (PRP). However, there has been widespread acceptance of PRP for use in clinical practice, despite an inadequate understanding of its biological mechanism of action. PURPOSE: To determine whether a single dose of PRP could accelerate ligament healing and correspondingly improve histological characteristics and biomechanical properties when injected immediately postoperatively into the injured medial collateral ligament (MCL) of New Zealand White rabbits...
December 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29210691/removing-2-4-dichlorophenol-from-aqueous-environments-by-heterogeneous-catalytic-ozonation-using-synthesized-mgo-nanoparticles
#12
Leili Mohammadi, Edris Bazrafshan, Meissam Noroozifar, Alireza Ansari-Moghaddam, Farahnaz Barahuie, Davoud Balarak
2,4-Dichlorophenol (2,4-DCP) is one of the seriously toxic chlorophenol compounds found in agricultural environments, in water disinfected by chlorine, and in outgoing effluents from the pulp and paper industries and paper manufacturing factories. This research studied the feasibility of using MgO nanoparticles (MgO-NPs) as a catalyst in the ozonation process for removing 2,4-DCP from aqueous environments under laboratory conditions. This study was conducted using a laboratory-scale semi-continuous reactor...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29209110/chitinase-3-like-1-secreted-by-peritumoral-macrophages-in-esophageal-squamous-cell-carcinoma-is-a-favorable-prognostic-factor-for-survival
#13
Shan Xing, Xin Zheng, Tao Zeng, Mu-Sheng Zeng, Qian Zhong, Yue-Song Cao, Kai-Lu Pan, Chu Wei, Fan Hou, Wan-Li Liu
AIM: To identify whether chitinase 3-like 1 (CHI3L1) serves as a suitable biomarker for the prognosis of esophageal squamous cell carcinoma (ESCC) and to analyze this protein's cellular source. METHODS: An ELISA was conducted to detect the concentration of CHI3L1 in the serum of 150 ESCC patients diagnosed between January 2001 and February 2005. The prognostic relevance of CHI3L1 was evaluated by a Kaplan-Meier and Cox regression analysis. The immunohistochemistry was reanalyzed, and fluorescent staining was utilized to explore the cellular origins of CHI3L1...
November 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29208557/demonstration-of-bioprocess-factors-optimization-for-enhanced-mono-rhamnolipid-production-by-a-marine-pseudomonas-guguanensis
#14
RamyaDevi K C, Lakshmi Sundaram R, Asha D, Sivamurugan V, Vidya Vasudevan, Mary Elizabeth Gnanambal Krishnan
We identified that Pseudomonas guguanensis produced macromolecular mono-rhamnolipid (Mol. Wt 1264.52Da) upon sensing n-hexadecane/diesel/kerosene from its surroundings. Permutation experiments were done to improve the laboratory-scale mono-rhamnolipid production (ie, a three-fold increase) using RSM validation. Consequently, maximal mono-rhamnolipids production [40-50mg/L] and emulsification abilities [65-70%] were encountered on day 8 using vegetable oil, peptone+yeast extract. EI24 values of the rhamnolipids were found to be 78±1...
December 2, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29207511/subcellular-trafficking-of-the-papillomavirus-genome-during-initial-infection-the-remarkable-abilities-of-minor-capsid-protein-l2
#15
REVIEW
Samuel K Campos
Since 2012, our understanding of human papillomavirus (HPV) subcellular trafficking has undergone a drastic paradigm shift. Work from multiple laboratories has revealed that HPV has evolved a unique means to deliver its viral genome (vDNA) to the cell nucleus, relying on myriad host cell proteins and processes. The major breakthrough finding from these recent endeavors has been the realization of L2-dependent utilization of cellular sorting factors for the retrograde transport of vDNA away from degradative endo/lysosomal compartments to the Golgi, prior to mitosis-dependent nuclear accumulation of L2/vDNA...
December 3, 2017: Viruses
https://www.readbyqxmd.com/read/29206630/does-untreated-obstructive-sleep-apnea-cause-secondary-erythrocytosis
#16
Christopher D Nguyen, Jon-Erik C Holty
BACKGROUND: The current literature suggests a relationship between obstructive sleep apnea (OSA) severity and hematocrit. However, the degree that OSA contributes to clinically significant erythrocytosis is uncertain. The aim of this study is to evaluate this association in a large study sample controlling for multiple confounders. METHODS: We evaluated consecutive subjects with suspected untreated OSA using multivariate analysis to test the associations between apnea-hypopnea index (AHI) and hematocrit...
September 2017: Respiratory Medicine
https://www.readbyqxmd.com/read/29206447/a-collection-method-for-isotopic-analysis-of-gaseous-nitrous-acid-hono
#17
Jiajue Chai, Meredith G Hastings
The sources and chemistry of gaseous nitrous acid (HONO) in the environment are of great interest. HONO is a major source of atmospheric hydroxyl radical (OH), which greatly impacts air quality and climate. HONO is also a major indoor pollutant that threatens human health. However, the large uncertainty of HONO sources and chemistry hinders an accurate prediction of the OH budget. Isotopic analysis of HONO may provide a tool for tracking the sources and chemistry of HONO. In this study, a modified annular denuder system (ADS) was developed to quantitatively capture HONO for offline nitrogen and oxygen isotopic analysis (δ15N and δ18O) using the denitrifier method...
December 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29205956/generating-hydrated-electrons-for-chemical-syntheses-with-a-green-led
#18
Robert Naumann, Florian Lehmann, Martin Goez
We present the first working system for accessing and utilizing laboratory-scale concentrations of hydrated electrons by photoredox catalysis with a green light-emitting diode (LED). Decisive are micellar compartmentalization and photon pooling in an intermediate that decays with second-order kinetics. The only consumable is the nontoxic and bioavailable vitamin C. A turnover number of 1380 shows the LED method to be on par with electron generation by high-power pulsed lasers, but at a fraction of the cost...
December 5, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29204688/pharmacokinetics-of-recombinant-asparaginase-in-children-with-acute-lymphoblastic-leukemia
#19
Swantje Völler, Uwe Pichlmeier, Anke Zens, Georg Hempel
PURPOSE: The objective of this study was to assess the pharmacokinetics of recombinant asparaginase (rASNase, Spectrila®) in children with acute lymphoblastic leukemia using a population pharmacokinetic approach in order to explore potential dosing recommendations. METHODS: Data on serum asparaginase activities of 124 children from three clinical studies were included in the analysis, covering an age range from 3 days to 17 years. Most patients received 5000 U/m2 rASNase intravenously every 3 days...
December 5, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/29204574/variables-associated-with-olfactory-disorders-in-adults-a-u-s-population-based-analysis
#20
Julia Noel, Al-Rahim R Habib, Andrew Thamboo, Zara M Patel
Objective: Olfactory dysfunction is known to have significant social, psychological, and safety implications. Despite increasingly recognized prevalence, potential risk factors for olfactory loss have been arbitrarily documented and knowledge is limited in scale. The aim of this study is to identify potential demographic and exposure variables correlating with olfactory dysfunction. Methods: Cross-sectional analysis of the 2011-2012 and 2013-2014 editions of the National Health Examination and Nutrition Survey was performed...
March 2017: World Journal of Otorhinolaryngology—Head and Neck Surgery
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