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https://www.readbyqxmd.com/read/28340117/an-evaluation-of-the-performance-of-the-dynamiker%C3%A2-fungus-1-3-%C3%AE-d-glucan-assay-to-assist-in-the-diagnosis-of-invasive-aspergillosis-invasive-candidiasis-and-pneumocystis-pneumonia
#1
P Lewis White, Jessica S Price, Raquel B Posso, Rosemary A Barnes
Invasive fungal disease (IFD) can be caused by a range of pathogens. Conventional diagnosis has the capacity to detect most causes of IFD, but poor performance limits impact. The introduction of non-culture diagnostics, including the detection of (1-3)-β-D-Glucan (BDG), has shown promising performance for the detection of IFD in variety of clinical settings. Recently, the Dynamiker® Fungus (1-3)-β-D-Glucan assay (D-BDG) was released as an IFD diagnostic test. This article describes an evaluation of the D-BDG assay for the diagnosis of invasive aspergillosis (IA), invasive candidiasis (IC) and Pneumocystis pneumonia (PCP) across several high-risk patient cohorts and provides comparative data with the Associates of Cape Cod Fungitell® and BioRad Platelia™ Aspergillus Ag (GM) assays...
March 3, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28339388/basics-of-compounding-clinical-pharmaceutics-part-2
#2
Loyd V Allen
This article represents part 2 of a 2-part article on the topic of clinical pharmaceutics. Pharmaceutics is relevant far beyond the pharmaceutical industry, compounding, and the laboratory. Pharmaceutics can be used to solve many clinical problems in medication therapy. A pharmacists' knowledge of the physicochemical aspects of drugs and drug products should help the patient, physician, and healthcare professionals resolve issues in the increasingly complex world of modern medicine. Part 1 of this series of articles discussed incompatibilities which can directly affect a clinical outcome and utilized pharmaceutics case examples of the application and importance of clinical pharmaceutics covering different characteristics...
November 2016: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/28339374/basics-of-compounding-clinical-pharmaceutics-part-1
#3
Loyd V Allen
Pharmaceutics is relevant far beyond the pharmaceutical industry, compounding, and the laboratory. Pharmaceutics can be used to solve many clinical problems in medication therapy. A pharmacists' knowledge of the physicochemical aspects of drugs and drug products should help the patient, physician, and healthcare professionals resolve issues in the increasingly complex world of modern medicine. Pharmacy is unique as it contains a knowledge base significantly different from that of physicians, nurses, and other health-related practitioners...
September 2016: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/28338388/preferred-hip-strategy-during-landing-reduces-knee-abduction-moment-in-collegiate-female-soccer-players
#4
Anh-Dung Nguyen, Jeffrey B Taylor, Taylor G Wimbish, Jennifer L Keith, Kevin R Ford
CONTEXT: Hip focused interventions are aimed to decrease frontal plane knee loading related to anterior cruciate ligament (ACL) injuries. Whether a preferred hip landing strategy decreases frontal plane knee loading is unknown. OBJECTIVE: To determine if a preferred hip landing strategy during a drop vertical jump (DVJ) is utilized during a single leg landing (SLL) task and whether differences in frontal plane knee loading are consistent between a DVJ and a SLL task...
March 24, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/28337628/study-of-plasma-off-gas-treatment-from-spent-ion-exchange-resin-pyrolysis
#5
Hernán Ariel Castro, Vittorio Luca, Hugo Luis Banchi
Polystyrene divinylbenzene-based ion exchange resins are employed extensively within nuclear power plants (NPPs) and research reactors for purification and chemical control of the cooling water system. To maintain the highest possible water quality, the resins are regularly replaced as they become contaminated with a range of isotopes derived from compromised fuel elements as well as corrosion and activation products including (14)C, (60)Co, (90)Sr, (129)I, and (137)Cs. Such spent resins constitute a major proportion (in volume terms) of the solid radioactive waste generated by the nuclear industry...
March 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28334326/allelopathy-mediated-competition-in-microbial-mats-from-antarctic-lakes
#6
Marc Slattery, Michael P Lesser
Microbial mats are vertically stratified communities that host a complex consortium of microorganisms, dominated by cyanobacteria, that compete for available nutrients and environmental niches, within these extreme habitats. The Antarctic Dry Valleys near McMurdo Sound include a series of lakes within the drainage basin that are bisected by glacial traverses. These lakes are traditionally independent, but recent increases in glacial melting have allowed two lakes (Chad and Hoare) to become connected by a meltwater stream...
February 18, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28333641/droplet-size-aware-high-level-synthesis-for-micro-electrode-dot-array-digital-microfluidic-biochips
#7
Zipeng Li, Kelvin Yi-Tse Lai, Po-Hsien Yu, Krishnendu Chakrabarty, Tsung-Yi Ho, Chen-Yi Lee
A digital microfluidic biochip (DMFB) is an attractive technology platform for automating laboratory procedures in biochemistry. In recent years, DMFBs based on a microelectrode-dot-array (MEDA) architecture have been demonstrated. However, due to the inherent differences between today's DMFBs and MEDA, existing synthesis solutions for biochemistry mapping cannot be utilized for MEDA biochips. We present the first synthesis approach that can be used for MEDA biochips. We first present a general analytical model for droplet velocity and validate it experimentally using a fabricated MEDA biochip...
March 21, 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28333487/adaptation-of-the-arizona-cognitive-task-battery-for-use-with-the-ts65dn-mouse-model-mus-musculus-of-down-syndrome
#8
Michael R Hunsaker, Genevieve K Smith, Raymond P Kesner
We propose and validate a clear strategy to efficiently and comprehensively characterize neurobehavioral deficits in the Ts65Dn mouse model of Down syndrome. This novel approach uses neurocognitive theory to design and select behavioral tasks that test specific hypotheses concerning the results of Down syndrome. In this article, we model the Arizona Cognitive Task Battery, used to study human populations with Down syndrome, in Ts65Dn mice. We observed specific deficits for spatial memory, impaired long-term memory for visual objects, acquisition and reversal of motor responses, reduced motor dexterity, and impaired adaptive function as measured by nesting and anxiety tasks...
March 23, 2017: Journal of Comparative Psychology
https://www.readbyqxmd.com/read/28333280/hcg-activates-the-erk1-2-mitogen-activated-protein-kinase-pathway-in-human-endometrial-stromal-cells-transiently-increasing-the-progesterone-receptor-and-regulating-genes-associated-with-endometrial-receptivity
#9
Alejandro Tapia-Pizarro, Sebastián Archiles, Felipe Argandoña, Cecilia Valencia, Keyla Zavaleta, María Cecilia Johnson, Reinaldo González-Ramos, Luigi Devoto
STUDY QUESTION: How does hCG signal in human endometrial stromal cells (ESCs) and what is its role in regulating ESC function? SUMMARY ANSWER: CG signaling in ESCs activates the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2) pathway through exchange protein activated by cAMP (Epac) and transiently increases progesterone receptor (PR) transcript and protein expression and its transcriptional function. WHAT IS KNOWN ALREADY: hCG is one of the earliest embryo-derived secreted signals in the endometrium, which abundantly expresses LH/hCG receptors...
March 14, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28333044/effects-of-c-n-ratio-on-nitrous-oxide-production-from-nitrification-in-a-laboratory-scale-biological-aerated-filter-reactor
#10
Qiang He, Yinying Zhu, Leilei Fan, Hainan Ai, Xiaoliu Huangfu, Mei Chen
Emission of nitrous oxide (N2O) during biological wastewater treatment is of growing concern. This paper reports findings of the effects of carbon/nitrogen (C/N) ratio on N2O production rates in a laboratory-scale biological aerated filter (BAF) reactor, focusing on the biofilm during nitrification. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and microelectrode technology were utilized to evaluate the mechanisms associated with N2O production during wastewater treatment using BAF...
March 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28333030/semen-quality-before-cryopreservation-and-after-thawing-in-543-patients-with-testicular-cancer
#11
Antonio MacKenna, Javier Crosby, Cristián Huidobro, Eduardo Correa, Gonzalo Duque
OBJECTIVE: The main objective of this study was to assess semen characteristics of patients with testicular cancer before cryopreservation and after thawing, to evaluate the consequences of this technique on sperm quality in patients with testicular cancer. METHODS: Five hundred eighty-nine samples from 543 patients with testicular cancer were cryopreserved between 1995 and 2015, one aliquot per patient was used for a thawing test to assess the impact of cryopreservation on sperm motility; semen analysis was performed before cryo preservation and after thawing, the result interpretation was carried out using the 2010 World Health Organization (WHO) Laboratory Manual, and consent forms were signed by the patients for freezing and when sperm was used for reproductive purposes...
February 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28330213/biodegradation-of-butyronitrile-and-demonstration-of-its-mineralization-by-rhodococcus-sp-mtb5
#12
Ismailsab Mukram, Masarbo Ramesh, T R Monisha, Anand S Nayak, T B Karegoudar
A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results reveal that 100, 98, and 88 % degradation was achieved for 2, 2.5, and 3 % butyronitrile, respectively. The strain is capable of growing in as high as 5 % butyronitrile concentration. A two-step pathway involving nitrile hydratase (NHase) and amidase was observed for the biodegradation of butyronitrile...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328623/a-novel-user-friendly-model-to-predict-corticosteroid-utilization-in-newly-diagnosed-patients-with-ulcerative-colitis
#13
Nabeel Khan, Dhruvan Patel, Yash Shah, Gary Lichtenstein, Yu-Xiao Yang
BACKGROUND: Corticosteroid (CS) use is an important marker of poor prognosis in ulcerative colitis (UC). Our aim was to develop and validate a model to predict the risk of CS utilization over the course of disease in newly diagnosed patients with UC. METHODS: Newly diagnosed patients with UC from a nationwide VA cohort were followed over time to evaluate factors predictive of CS use. Multivariate logistic regression was performed. Model development was performed in a random 2/3 of the total cohort and then validated in the remaining 1/3...
March 21, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28327156/mycobacterium-goodii-endocarditis-following-mitral-valve-ring-annuloplasty
#14
Rohan B Parikh, Matthew Grant
BACKGROUND: Mycobacterium goodii is an infrequent human pathogen which has been implicated in prosthesis related infections and penetrating injuries. It is often initially misidentified as a gram-positive rod by clinical microbiologic laboratories and should be considered in the differential diagnosis. CASE PRESENTATION: We describe here the second reported case of M. goodii endocarditis. Species level identification was performed by 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing...
March 21, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28325786/impact-on-hospital-resources-of-systematic-evaluation-and-management-of-suspected-nonaccidental-trauma-in-patients-less-than-4-years-of-age
#15
Bethann M Pflugeisen, Mauricio A Escobar, Dustin Haferbecker, Yolanda Duralde, Elizabeth Pohlson
OBJECTIVE: There has been an increasing movement worldwide to create systematic screening and management procedures for atypical injury patterns in children with the hope of better detecting and evaluating nonaccidental trauma (NAT). A legitimate concern for any hospital considering implementation of a systematic evaluation process is the impact on already burdened hospital resources. We hypothesized that implementation of a guideline that uses red flags related to history, physical, or radiologic findings to trigger a standardized NAT evaluation of patients <4 years would not negatively affect resource utilization at our level II pediatric trauma center...
March 21, 2017: Hospital Pediatrics
https://www.readbyqxmd.com/read/28325018/imquant-an-image-based-fluorescence-reader-for-quantitative-lateral-flow-immunoassays
#16
Kaushik Basak Chowdhury, Jayaraj Joseph, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam
Fluorescence immunoassays (FIA) have strong potential in enabling rapid quantitative immunological testing at point of care. Existing state of the art instruments employ laser scanning of FIA test kits, which require the use of electromechanical systems, making the instrument bulky, heavy and less reliable in the long term. There is a growing need for an extremely compact and reliable, motion-free, automated reader for accurate quantification of immunoassays. Here, we present ImQuant, an image based FIA reader with integrated monochromatic excitation source, optical filtering and real time image capture, assisted with an intuitive graphical user interface...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28325017/diffuse-reflectance-spectroscopy-can-differentiate-high-grade-and-low-grade-prostatic-carcinoma
#17
Priya N Werahera, Edward A Jasion, E David Crawford, M Scott Lucia, Adrie van Bokhoven, Holly T Sullivan, Fernando J Kim, Paul D Maroni, J David Port, John W Daily, Francisco G La Rosa
Prostate tumors are graded by the revised Gleason Score (GS) which is the sum of the two predominant Gleason grades present ranging from 6-10. GS 6 cancer exclusively with Gleason grade 3 is designated as low grade (LG) and correlates with better clinical prognosis for patients. GS >7 cancer with at least one of the Gleason grades 4 and 5 is designated as HG indicate worse prognosis for patients. Current transrectal ultrasound guided prostate biopsies often fail to correctly diagnose HG prostate cancer due to sampling errors...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324620/exposure-to-electromagnetic-fields-from-smart-utility-meters-in-gb-part-i-laboratory-measurements
#18
Azadeh Peyman, Darren Addison, Terry Mee, Cristian Goiceanu, Myron Maslanyj, Simon Mann
Laboratory measurements of electric fields have been carried out around examples of smart meter devices used in Great Britain. The aim was to quantify exposure of people to radiofrequency signals emitted from smart meter devices operating at 2.4 GHz, and then to compare this with international (ICNIRP) health-related guidelines and with exposures from other telecommunication sources such as mobile phones and Wi-Fi devices. The angular distribution of the electric fields from a sample of 39 smart meter devices was measured in a controlled laboratory environment...
March 21, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/28322802/simple-quantitative-pcr-analysis-for-allelic-pten-loss-in-tumor-progression
#19
Xingping Wu, Ailin Zhu, Xiaowu Pang, Guiqin Xie, Xinbin Gu
We describe a simple method to accurately detect and quantify both Pten mutation and allele-specific loss using allele-specific PCR analysis. Our approach used a heterozygous genomic DNA with one wild-type and one mutant Pten allele as a reference at a single concentration to calculate the percent ratio of the wild-type Pten gene for the detection of allele-specific gene loss. With a standard curve, ratios from PCR data were used to quantitate the wild-type Pten allele copy number loss in tumor specimens. We demonstrate the utility of our approach to calculate allele-specific Pten loss during tumor progression and show that our approach generates quantitative data that are comparable to those obtained from digital droplet PCR...
March 18, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28322757/impaired-clinical-utility-of-sequential-patient-gem-blood-gas-measurements-associated-with-calibration-schedule
#20
George S Cembrowski, Qian Xu, Adam R Cembrowski, Junyi Mei, Hossein Sadrzadeh
BACKGROUND: Within- and/or between-instrument variation may falsely indicate patient trends or obscure real trends. We employ a methodology that transforms sequential intra-patient results into estimates of biologic and analytic variation. We previously derived realistic biologic variation (sb) of blood gas (BG) and hematology analytes. We extend this methodology to derive the imprecision of two GEM 4000 BG analyzers. METHODS: A laboratory data repository provided arterial BG, electrolyte and metabolite results generated by two GEM 4000s on ICU patients in 2012-2013...
March 18, 2017: Clinical Biochemistry
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