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https://www.readbyqxmd.com/read/28546437/characterization-of-thermostable-cam-rubisco-activase-reveals-a-rubisco-interacting-surface-loop
#1
Devendra Shivhare, Oliver Mueller-Cajar
In order to maintain metabolic flux through the Calvin-Benson-Bassham (CBB) cycle in higher plants, dead-end inhibited complexes of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) must constantly be engaged and remodeled by the molecular chaperone Rubisco activase (Rca). In C3 plants the thermolability of Rca is responsible for the deactivation of Rubisco and reduction of photosynthesis at moderately elevated temperatures. We reasoned that CAM plants must possess thermostable Rca to support CBB flux during the day when stomata are closed...
May 25, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28543370/overexpression-of-the-rubisco-activase-gene-improves-growth-and-low-temperature-and-weak-light-tolerance-in-cucumis-sativus-l
#2
Huangai Bi, Peipei Liu, Zhensheng Jiang, Xizhen Ai
Rubisco activase (RCA) is an important enzyme that can catalyze the carboxylation and oxygenation activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which is involved in the photosynthetic carbon reduction cycle. Here, we studied the effects of changes in RCA activity on photosynthesis, growth and development, as well as the chilling tolerance of RCA overexpressing transgenic cucumber (Cucumis sativus) plants. CsRCA overexpression increased the plant height, leaf area and dry matter, and decreased the root/top ratio in transgenic cucumber plants compared with the wild type (WT) plants...
May 22, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28536711/a-target-triggered-proximity-combination-based-fluorescence-sensing-strategy-for-adenosine-detection
#3
Xiaowen Xu, Haiping Wei, Wei Jiang
Adenosine is a potent physiological and pharmacological regulator, and its abnormal level is closely related to disease development. The sensitive and specific detection of adenosine is crucial for health evaluation and disease diagnosis. In this work, a target triggered proximity combination-based fluorescence sensing strategy is developed for the sensitive and specific detection of adenosine. A difunctional probe showing target recognition and signal amplification is designed, by integration of DNA linker-connected split aptamer fragments with a fragment-elongated polymerase/nicking template...
May 24, 2017: Analyst
https://www.readbyqxmd.com/read/28536071/analysis-of-anelloviridae-sequences-characterized-from-serial-human-and-animal-biological-samples
#4
Sandra Bédarida, Bertrand Dussol, Michel Signoli, Philippe Biagini
Rolling-circle amplification-sequence-independent single primer amplifications (RCA-SISPA) and/or RCA-PCR-based approaches were applied to serial human plasma and animal (domestic cat) saliva samples. Complete SENV-H-related and PRA4 Anelloviridae genomes were characterized and analysed over time (~16 and 6.5years for human and animal samples, respectively). Genomic sequences and deduced putative coding regions were compared. Comparable values, i.e. ~2×10(-4)subs/site/year, were obtained for estimated rates of non-synonymous substitutions...
May 20, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28535516/citrate-anticoagulation-during-continuous-renal-replacement-therapy
#5
Davide Ricci, Laura Panicali, Maria Grazia Facchini, Elena Mancini
During extracorporeal dialysis, some anticoagulation strategy is necessary to prevent the coagulation of blood. Heparin has historically been used as an anticoagulant because of its efficacy combined with low cost. However, a variable incidence of hemorrhagic complications (5-30%) has been documented in patients undergoing continuous renal replacement therapy (CRRT) with heparin as an anticoagulant. Citrate has anticoagulation properties secondary to its ability to chelate calcium, which is necessary for the coagulation cascade...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/28533990/anomalous-origin-of-right-coronary-artery-originating-from-the-pulmonary-trunk-arcapa-an-incidental-finding-in-a-patient-presenting-with-chest-pain
#6
Pragathi Balakrishna, Michael Illovsky, Youssef M Al-Saghir, Abdul M Minhas
Anomalous origin of the right coronary artery originating from the pulmonary trunk (ARCAPA) is a rare congenital coronary anomaly with an estimated prevalence of 0.002%. Most patients are asymptomatic and the anomaly is detected incidentally during evaluation for other problems. Occasionally, ARCAPA may lead to myocardial ischemia and/or sudden cardiac arrest. We present a case of a 55-year-old female with a history of hypertension who presented to the emergency department with intermittent chest discomfort for three days...
April 17, 2017: Curēus
https://www.readbyqxmd.com/read/28533097/distal-anastomosis-support-for-coronary-artery-bypass-grafting-a-new-surgical-technique-and-current-outcomes
#7
Junfeng Yang, Liangshan Wang, Haitao Li, Changcheng Liu, Bo Li, Hui Li, Rui Liu, Chengxiong Gu
BACKGROUND: Saphenous vein graft (SVG) failure remains the Achilles' heel of coronary artery bypass grafting (CABG) and grafts performed on the right coronary artery (RCA) system always have the worst patency compared with those performed on the left coronary system. The aim of this study was to introduce the distal anastomoses support (DAS) procedure and investigate the effect of DAS on the mid-term graft patency of SVG-PDA. METHODS: Between May and September 2013, 100 patients with an RCA severe lesion who underwent isolated OPCABG (CABG alone group, n=50) or CABG with DAS for anastomoses of SVG-PDA (CABG plus DAS group, n=50) were evaluated retrospectively...
November 30, 2016: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28528624/root-cause-analysis-of-adverse-events-in-an-outpatient-anticoagulation-management-consortium
#8
Christopher M Graves, Brian Haymart, Eva Kline-Rogers, Geoffrey D Barnes, Linda K Perry, Denise Pluhatsch, Nannette Gearhart, Helen Gikas, Noelle Ryan, Brian Kurtz
BACKGROUND: A number of factors can lead to adverse events (AEs) in patients taking warfarin. Performing a root cause analysis (RCA) of serious AEs is one systematic way of determining the causes of these events. METHODS: Multidisciplinary teams were formed at Michigan Anticoagulation Quality Improvement Initiative (MAQI(2)) sites with organized anticoagulation management services (AMS). Medical records from patients who suffered serious AEs (major bleed, embolic stroke, venous thromboembolism) were reviewed, and AMS staff were interviewed to determine the root cause using the "5 Whys" technique...
June 2017: Joint Commission Journal on Quality and Patient Safety
https://www.readbyqxmd.com/read/28528054/surgical-management-of-simultaneous-left-coronary-atresia-and-anomalous-right-coronary-artery-origin
#9
Shyam Sathanandam, T K Susheel Kumar, Umar Boston, Christopher J Knott-Craig
A 9-year-old child presented with syncope during exercise. He received a diagnosis of congenital atresia of the left main coronary artery by angiography. He underwent successful coronary artery bypass grafting. On the third postoperative day, he experienced acute, precordial chest pain. An urgent computed tomographic scan showed an unrecognized anomalous origin of the right coronary artery (RCA) with a 1.5-cm intramural course. He was taken back to the operating room to undergo unroofing of the RCA. This case highlights the difficulty involved in making two rare diagnoses that can cause the same exact symptoms in a patient and the surgical challenges associated with it...
June 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28521602/dynamic-alterations-in-cerebral-celiac-and-renal-flows-resulting-from-ascending-aorta-subclavian-artery-and-femoral-artery-cannulations-of-extracorporeal-devices
#10
Sahin Iscan, Habib Cakir, Bortecin Eygi, Ismail Yurekli, Koksal Donmez, Pınar Unde Ayvat, Derya Sarıkaya Pekel, Mert Kestelli
INTRODUCTION: The aim of this study was to assess the relationships among cardiac output, extracorporeal blood flow, cannulation site, right (RCa) and left carotid (LCa), celiac (Ca) and renal artery (Ra) flows during extracorporeal circulation. METHODS: A mock circulatory circuit was assembled, based on a compliant anatomical aortic model. The ascending aorta, right subclavian and femoral artery cannulations were created and flow was provided by a centrifugal pump (Cp); cardiac output was provided by a roller pump (Rp)...
May 1, 2017: Perfusion
https://www.readbyqxmd.com/read/28520537/anomalous-origin-of-right-coronary-artery-from-left-coronary-sinus-13-cases-treated-with-the-reimplantation-technique
#11
Alain Cubero, Alejandro Crespo, Gadah Hamzeh, Andrés Cortes, Daniel Rivas, José Ignacio Aramendi
OBJECTIVES: Anomalous aortic origin of a coronary artery is uncommon but potentially clinically significant. Manifestations vary from asymptomatic patients to those who present with angina pectoris, myocardial infarction, heart failure, syncope, arrhythmias, and sudden death. We describe our experience with surgical reimplantation and results at midterm follow-up. METHODS: Between February 2003 and July 2016, a total of 13 patients with anomalous origin of the right coronary artery (RCA) from the left sinus underwent surgical reimplantation...
May 2017: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/28517107/right-coronary-artery-coronary-sinus-fistula-with-coronary-sinus-ostium-stenosis
#12
Lihong Pu, Rongjuan Li, Ya Yang, Guowen Liu, Yueli Wang
Coronary artery fistula (CAF) is a rare anomaly of the coronary artery. The draining site of a right coronary artery (RCA) fistula may usually be the right ventricle, right atrium, or pulmonary artery. Here, we present a patient with right coronary artery to coronary sinus fistula (RCACSF) complicated by aneurysmal dilatation of the coronary sinus (CS) and stenosis of CS ostium.
May 18, 2017: Echocardiography
https://www.readbyqxmd.com/read/28515822/primary-percutaneous-coronary-intervention-angioplasty-of-occluded-twin-circumflex-coronary-artery-in-a-patient-of-acute-inferior-wall-myocardial-infarction-a-rare-anomaly
#13
Santosh Kumar Sinha, Vikas Mishra, Nasar Abdali, Mukesh Jitendra Jha, Mahmadula Razi, Shravan Singh, Lokendra Rekwal, Vikas Chaturvedi, Chandra Mohan Varma, Ramesh Thakur
Anomalies of the coronary arteries are reported in 1-2% of patients among diagnostic angiogram. Dual origin of a circumflex from both sinuses is extremely rare among them. We report a case of a patient who underwent primary percutaneous coronary intervention for acute inferior wall myocardial infarction where left coronary injection demonstrated normal obtuse marginal and right coronary injection demonstrated normal right coronary artery (RCA). On further probing, an anomalous left circumflex (LCx) artery was seen arising from RCA ostium which was subsequently cannulated and revascularized by deployment of 2...
April 2017: Cardiology Research
https://www.readbyqxmd.com/read/28511806/st-segment-elevation-myocardial-infarction-could-be-the-primary-presentation-of-acute-aortic-dissection
#14
Qing-Yi Zhu, Shi Tai, Liang Tang, Wen Peng, Sheng-Hua Zhou, Zhen-Guo Liu, Xin-Qun Hu
BACKGROUND: Stanford type A aortic dissection (TAAD) may lead to coronary artery occlusion and malfunction. However, TAAD manifesting as acute ST-segment elevation myocardial infarction (STEMI) has not been studied. In the present study, we reported 8 TAAD cases with STEMI as the primary presentation, and analyzed their clinical characteristics and outcome. METHODS: The records were reviewed for patients admitted to the large comprehensive university hospital for PCI due to STEMI from January 1, 2002 to January 1, 2017...
May 11, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28510720/effects-of-a-3d-segmental-prosthetic-system-for-tricuspid-valve-annulus-remodelling-on-the-right-coronary-artery-a-human-cadaveric-coronary-angiography-study
#15
Mohsen Riki-Marishani, Arash Gholoobi, Ghasem Sazegar, Mathias H Aazami, Aria Hedjazi, Maryam Sajjadian, Mahmoud Ebrahimi, Ahmad Aghaii-Zade Torabi
OBJECTIVES: A prosthetic system to repair secondary tricuspid valve regurgitation was developed. The conceptual engineering of the current device is based on 3D segmental remodelling of the tricuspid valve annulus in lieu of reductive annuloplasty. This study was designed to investigate the operational safety of the current prosthetic system with regard to the anatomical integrity of the right coronary artery (RCA) in fresh cadaveric human hearts. METHODS: During the study period, from January to April 2016, the current prosthetic system was implanted on the tricuspid valve annulus in fresh cadaveric human hearts that met the study's inclusion criteria...
May 16, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28499664/stemi-with-fluctuating-closing-of-lad-and-cx-do-not-concentrate-on-the-finger
#16
Mario Sadaba, Asier Subinas, Jose Ramón Rumoroso
Case report of the male with an anterior STEMI to whom a primary PCI is performed. The angiogram shows a fluctuating close of the LAD and Cx than when an OCT is performed does not clearly see any pathological findings but when the IVUS is performed, it is clearly seen as a coronary haematoma at the LM, LAD and Cx. Stent at the LM-LAD and proximal Cx are implanted with final good result. After exchanging the guiding catheter for a diagnostic catheter to visualize the RCA, there is an aortic flap; the coronary haematoma was coming from an ascending aortic dissection...
March 27, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/28492073/polyvalent-and-thermosensitive-dna-nanoensembles-for-cancer-cell-detection-and-manipulation
#17
Jinlu Tang, Yanru Yu, Hui Shi, Xiaoxiao He, Yanli Lei, Jingfang Shangguan, Xue Yang, Zhenzhen Qiao, Kemin Wang
Development of smart DNA nanostructures is of great value in cancer studies. Here, by integrating rolling circle amplification (RCA) into split aptamer design, a novel strategy of polyvalent and thermosensitive DNA nanoensembles was first proposed for cancer cell detection and manipulation. In this strategy, a long nanosolo ssDNA with repeated Split-b and Poly T regions was generated through RCA. Split-b supplied polyvalent binding sites while Poly T supported signal output by hybridizing with fluorophore-labeled poly A...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28482382/-application-of-improved-regional-citrate-anticoagulation-in-continuous-hemofiltration-in-children
#18
K Bai, C J Liu, Y Q Fu, F Xu
Objective: To investigate the application of regional citrate anticoagulation with calcium hemofiltration basic solution in continuous hemofiltration in children. Method: The clinical data of 18 patients with citrate anticoagulation in continuous hemofiltration in children, excluding the hepatic failure and septic shock cases, were analyzed retrospectively, from September 2015 to August 2016 in Intensive Care Unit of the Children's Hospital of Chongqing Medical University.The commercial calcium hemofiltration basic solution was used as displacement liquid ...
May 4, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28476515/independent-predictors-of-in-stent-restenosis-after-drug-eluting-stent-implantation-for-ostial-right-coronary-artery-lesions
#19
Yusuke Watanabe, Kensuke Takagi, Toru Naganuma, Hiroyoshi Kawamoto, Yusuke Fujino, Hisaaki Ishiguro, Satoko Tahara, Naoyuki Kurita, Koji Hosawa, Shotaro Nakamura, Sunao Nakamura
OBJECTIVES: We evaluated the angiographic patterns and predictors of in-stent restenosis (ISR) for ostial lesions of the right coronary artery (RCA) to clarify the mechanism of insoluble restenosis. BACKGROUND: Although ISR of the RCA still occurs, limited data is available regarding the associated angiographic findings. METHODS: Between January 2005 and September 2013, we recruited consecutive patients undergoing routine angiography 6-18months after implantation of a drug-eluting stent (DES)...
April 26, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28475171/relationship-between-infarct-artery-location-acute-total-coronary-occlusion-and-mortality-in-stemi-and-nstemi-patients
#20
Jarosław Karwowski, Marek Gierlotka, Mariusz Gąsior, Lech Poloński, Jan Ciszewski, Maciej Bęćkowski, Ilona Kowalik, Hanna Szwed
INTRODUCTION    Prevalence and impact of total coronary occlusion of an infarct-related artery (IRA) on outcome could be different between patients with Non-ST-Elevation Myocardial Infarction (NSTEMI) and ST-segment elevation myocardial infarction (STEMI) undergoing percutaneous coronary intervention (PCI). OBJECTIVES    We evaluated the impact of IRA occlusion on outcome of myocardial infarction, according to the presence or absence of ST-segment elevation and the culprit lesion location. PATIENTS AND METHODS    We analyzed 4581 STEMI patients and 2717 NSTEMI patients enrolled in the Polish Registry of Acute Coronary Syndromes who underwent PCI...
May 5, 2017: Polish Archives of Internal Medicine
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