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https://www.readbyqxmd.com/read/29139059/evolution-of-rubisco-activase-gene-in-plants
#1
Ragupathi Nagarajan, Kulvinder S Gill
Rubisco activase of plants evolved in a stepwise manner without losing its function to adapt to the major evolutionary events including endosymbiosis and land colonization. Rubisco activase is an essential enzyme for photosynthesis, which removes inhibitory sugar phosphates from the active sites of Rubisco, a process necessary for Rubisco activation and carbon fixation. The gene probably evolved in cyanobacteria as different species differ for its presence. However, the gene is present in all other plant species...
November 14, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29134268/clinical-significance-of-transluminal-attenuation-gradient-in-320-row-area-detector-coronary-ct-angiography
#2
Etsuro Kato, Shinichiro Fujimoto, Kazuhisa Takamura, Yuko Kawaguchi, Chihiro Aoshima, Makoto Hiki, Kanako K Kumamaru, Hiroyuki Daida
The clinical significance of the transluminal attenuation gradient (TAG) has not been established. We evaluated the incremental diagnostic value of TAG by 320-row area detector computed tomography (320-ADCT). Subjects were 65 patients who underwent one-rotation scanning by 320-ADCT and invasive coronary angiography (ICA) within 3 months. TAG values were obtained for the major epicardial vessels 2 mm or more each in RCA, LAD and LCX using automatic analysis software. Moreover, TAG values that excluded calcified lesions in calculation of the regression line were also evaluated (excluded-TAG)...
November 13, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/29119154/terahertz-spectroscopy-for-the-isothermal-detection-of-bacterial-dna-by-magnetic-bead-based-rolling-circle-amplification
#3
Xiang Yang, Ke Yang, Xiang Zhao, Zhongquan Lin, Zhiyong Liu, Sha Luo, Yang Zhang, Yunxia Wang, Weiling Fu
The demand for rapid and sensitive bacterial detection is continuously increasing due to the significant requirements of various applications. In this study, a terahertz (THz) biosensor based on rolling circle amplification (RCA) was developed for the isothermal detection of bacterial DNA. The synthetic bacterium-specific sequence of 16S rDNA hybridized with a padlock probe (PLP) that contains a sequence fully complementary to the target sequence at the 5' and 3' ends. The linear PLP was circularized by ligation to form a circular PLP upon recognition of the target sequence; then the capture probe (CP) immobilized on magnetic beads (MBs) acted as a primer to initialize RCA...
November 9, 2017: Analyst
https://www.readbyqxmd.com/read/29117919/guideliner-extension-catheter-facilitated-side-strut-stenting-technique-for-the-treatment-of-right-coronary-artery-ostial-in-stent-restenosis
#4
REVIEW
George Kassimis, Tushar Raina
Percutaneous coronary intervention (PCI) for the treatment of right coronary artery (RCA) ostial in-stent restenosis (ISR) can be challenging. The technical difficulties are compounded by excessive stent protrusion into the aorta, preventing coaxial engagement of the guiding catheter and wiring through the central stent lumen. Through this review, we aim to highlight the practical challenges encountered during the treatment of ostial RCA ISR through PCI and evaluate the various interventional techniques that have been used...
September 22, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29102344/clinical-outcomes-of-coronary-occlusion-following-transcatheter-aortic-valve-replacement-a-systematic-review
#5
REVIEW
Oluwaseun A Akinseye, Sunil K Jha, Uzoma N Ibebuogu
BACKGROUND: Coronary occlusion (CO) is a rare but serious complication following transcatheter aortic valve replacement (TAVR) with limited published data. We sought to evaluate the immediate and short-term outcomes of CO complicating TAVR. METHODS: Studies, including case reports, case series and original articles published from 2002 to 2016 describing CO following TAVR were identified with a systematic electronic search using the PRISMA Statement. Only studies reporting data on demographic and procedural characteristics, management and follow up outcomes were analyzed...
September 12, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29098379/influence-of-hydrostatic-pressure-on-intracoronary-indices-of-stenosis-severity-in-vivo
#6
Tobias Härle, Mareike Luz, Sven Meyer, Felix Vahldiek, Pim van der Harst, Randy van Dijk, Daan Ties, Javier Escaned, Justin Davies, Albrecht Elsässer
BACKGROUND: An influence of hydrostatic pressure on intracoronary indices of stenosis severity in vitro was recently reported. We sought to analyze the influence of hydrostatic pressure, caused by the height difference between the distal and proximal pressure sensor after guidewire positioning in the interrogated vessel, on intracoronary pressure measurements in vivo. METHODS AND RESULTS: In 30 coronary stenoses, intracoronary pressure measurements were performed in supine, left, and right lateral patient position...
November 2, 2017: Clinical Research in Cardiology: Official Journal of the German Cardiac Society
https://www.readbyqxmd.com/read/29098093/epoetin-alfa-a-cause-of-coronary-artery-thrombosis
#7
Muhammad Umer Siddiqui, Yelena Galumyan, James Klein, Zunaira Naeem, Aron Schwarcz
Introduction: Epoetin alfa is an erythrocyte-stimulating factor. We here present a case of an anemic patient, who was given epogen before a coronary artery bypass (CABG) surgery and developed periprocedural myocardial infarction. To our knowledge, there has been no previous case reported of epogen causing myocardial infarction. Case Presentation: 66-year-old female presented with substernal chest pain. EKG showed ST segment elevations in aVf and L-III. Patient underwent left heart catheterization (LHC) and had triple vessel disease...
2017: Case Reports in Cardiology
https://www.readbyqxmd.com/read/29091948/selection-and-validation-of-appropriate-reference-genes-for-quantitative-real-time-pcr-analysis-in-salvia-hispanica
#8
Rahul Gopalam, Sunny D Rupwate, Ajay W Tumaney
Quantitative real-time polymerase chain reaction (qRT-PCR) has become the most popular choice for gene expression studies. For accurate expression analysis, it is pertinent to select a stable reference gene to normalize the data. It is now known that the expression of internal reference genes varies considerably during developmental stages and under different experimental conditions. For Salvia hispanica, an economically important oilseed crop, there are no reports of stable reference genes till date. In this study, we chose 13 candidate reference genes viz...
2017: PloS One
https://www.readbyqxmd.com/read/29084390/molecular-identification-of-i-neofabraea-i-species-associated-with-bull-s-eye-rot-on-apple-using-rolling-circle-amplification-of-partial-i-ef-1%C3%AE-i-sequence
#9
Huijiao Lin, Xiang Jiang, Jianping Yi, Xinguo Wang, Ranling Zuo, Zide Jiang, Weifang Wang, Erxun Zhou
A rolling-circle amplification (RCA) method with padlock probes targeted on <i>EF-1α</i> regions was developed for rapid detection of apple bull's eye rot (BER) pathogens, including<i> Neofabraea malicorticis</i>,<i> N. perennans</i>, <i>N. kienholzii</i> and <i>N. vagabunda</i> (synonym:<i> N. alba</i>). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species and 2 unrelated species which may cause serious apple decays...
October 30, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29071753/dna-hydrogel-with-tunable-ph-responsive-properties-produced-by-rolling-circle-amplification
#10
Wanlin Xu, Yishun Huang, Haoran Zhao, Pan Li, Guoyuan Liu, Jing Li, Chengshen Zhu, Tian Leilei
Recently, smart DNA hydrogels, which are generally fully self-assembled from oligonucleotides or cross-linked from oligonucleotide-polymer hybrids, have attracted tremendous attention. However, the difficulties of fabricating DNA hydrogels limit their practical applications. In this study, we report a novel method to produce pH-responsive hydrogels by rolling circle amplification (RCA). pH-Sensitive crosslinking sites are introduced into the polymeric DNA chains during RCA. The properties of the resulting hydrogel can be easily tuned through the template design...
October 25, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29068132/ostial-right-coronary-chronic-total-occlusion-transesophageal-echocardiographic-guidance-for-retrograde-aortic-re-entry
#11
Frank E Corrigan, Dimitrios Karmpaliotis, Habib Samady, Stamatios Lerakis
As antegrade options are limited, intervention upon the ostial right coronary artery (RCA) chronic total occlusion (CTO) warrants a retrograde approach. Landmarks for an aggressive approach are concerning as passage of stiff guidewires or electrocautery near the RCA ostium may result in wire passage into structures other than the aorta. We report the first use of transesophageal echocardiography (TEE) to assist retrograde passage of a guidewire into the aorta. For the ostial RCA CTO, TEE guidance may be considered to assist retrograde passage of aggressive guidewires into the aorta...
October 25, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29065341/bio-bar-code-based-photoelectrochemical-immunoassay-for-sensitive-detection-of-prostate-specific-antigen-using-rolling-circle-amplification-and-enzymatic-biocatalytic-precipitation
#12
Kangyao Zhang, Shuzhen Lv, Zhenzhen Lin, Meijin Li, Dianping Tang
Methods based on photoelectrochemistry have been developed for immunoassay, but most involve in a low sensitivity and a relatively narrow detectable range. Herein a new bio-bar-code-based split-type photoelectrochemical (PEC) immunoassay was designed for sensitive detection of prostate-specific antigen (PSA), coupling rolling circle amplification (RCA) with enzymatic biocatalytic precipitation. The bio-bar-code-based immunoreaction was carried out on monoclonal anti-PSA antibody (mAb1)-coated microplate using primer DNA and polyclonal anti-PSA antibody-conjugated gold nanoparticle (pDNA-AuNP-pAb2) with a sandwich-type assay format...
October 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29062445/the-impact-of-three-dimensional-reconstruction-on-laparoscopic-assisted-surgery-for-right-sided-colon-cancer
#13
Ryoichi Miyamoto, Sosuke Tadano, Naoki Sano, Satoshi Inagawa, Shinya Adachi, Masayoshi Yamamoto
INTRODUCTION: During laparoscopic-assisted colorectal surgery (LACS) for right-sided colon cancer patients, we performed three-dimensional (3D) surgical simulation to investigate vascular anatomy, including the ileocolic artery (ICA), right colic artery (RCA) and superior mesenteric vein (SMV). AIM: We also used 3D imaging to examine the shortest distance from the root of the ileocolic vein (ICV) to the gastrocolic trunk (GCT). MATERIAL AND METHODS: We analyzed 46 right-sided colon cancer patients who underwent 3D-simulated LACS...
September 2017: Wideochirurgia i Inne Techniki Mało Inwazyjne, Videosurgery and Other Miniinvasive Techniques
https://www.readbyqxmd.com/read/29061547/randomized-comparison-of-judkins-vs-tiger-catheter-in-coronary-angiography-via-the-right-radial-artery-the-judge-study
#14
Ioanna Xanthopoulou, Katerina Stavrou, Periklis Davlouros, Grigorios Tsigkas, Eleni Koufou, Georgios Almpanis, Michalis Koutouzis, Ioannis Tsiafoutis, Angelos Perperis, Athanasios Moulias, Nikolaos Koutsogiannis, George Hahalis
AIMS: To compare the performance of Tiger-II compared with Judkins 3.5L/4R catheters in coronary angiography (CAG) via the transradial approach (TRA). METHODS AND RESULTS: Consecutive patients undergoing non-urgent CAG via the right TRA were randomized to either Tiger-II (Terumo) or Judkins (3.5L/4R, Medtronic) 5Fr catheters and 320 patients in each group were randomized. Catheter or access site change was required in 57(17.8%) vs 68(21.3%) patients allocated in the Tiger-II and Judkins group respectively (p=0...
October 24, 2017: EuroIntervention
https://www.readbyqxmd.com/read/29056373/jte-852-a-novel-spleen-tyrosine-kinase-inhibitor-blocks-immunoglobulin-g-mediated-cellular-responses-and-autoimmune-reactions-in-vivo
#15
Toshinobu Kato, Takeshi Ohta, Hidenori Iwasaki, Hatsue Kobayashi, Akira Matsuo, Takahiro Hata, Mutsuyoshi Matsushita
AIMS: Immune and inflammatory responses mediated by immunoglobulin (Ig) G are largely responsible for the pathogenesis of autoimmune diseases. Spleen tyrosine kinase (Syk) plays a pivotal role in the IgG-mediated responses; therefore, Syk has emerged as a new therapeutic target for the treatment of autoimmune diseases. In this study, we investigated the inhibitory actions of JTE-852, a novel Syk inhibitor, on IgG-mediated cellular responses and autoimmune reactions in vivo. MAIN METHODS: We examined mediator secretion from human monocytes...
October 19, 2017: Life Sciences
https://www.readbyqxmd.com/read/29056178/root-cause-analysis-of-icu-adverse-events-in-the-veterans-health-administration
#16
Gregory S Corwin, Peter D Mills, Hasan Shanawani, Robin R Hemphill
BACKGROUND: ICUs' provision of complex care for critically ill patients results in an environment with a high potential for adverse events. A study was conducted to characterize adverse events in Veterans Health Administration (VHA) ICUs that underwent root cause analysis (RCA) and to identify the root causes and their recommended actions. METHODS: This retrospective observational study of RCA reports concerned events that occurred in VHA ICUs or as a result of ICU processes from January 1, 2013, through December 31, 2014...
November 2017: Joint Commission Journal on Quality and Patient Safety
https://www.readbyqxmd.com/read/29055232/adverse-events-occurring-on-mental-health-units
#17
Peter D Mills, Bradley V Watts, Brian Shiner, Robin R Hemphill
OBJECTIVE: While the study of suicide on mental health units has a long history, the study of patient safety more generally is relatively new. Our objective was to determine the type and relative frequency of adverse events occurring on Veterans Health Administration (VHA) mental health units; the primary root causes for these events; and make recommendations for abating or mitigating these events. METHODS: We searched our national database for any reported adverse event that occurred on an inpatient mental health unit between January 1, 2015 and December 31, 2016...
September 9, 2017: General Hospital Psychiatry
https://www.readbyqxmd.com/read/29053159/proximity-hybridization-triggered-rolling-circle-amplification-for-sensitive-electrochemical-homogeneous-immunoassay
#18
Fenglei Gao, Fuyi Zhou, Songjie Chen, Yao Yao, Jing Wu, Dengyang Yin, Deqin Geng, Po Wang
A new homogeneous electrochemical immunoassay strategy was developed for ultrasensitive detection of carcinoembryonic antigen (CEA) based on target-induced proximity hybridization coupled with rolling circle amplification (RCA). The immobilization-free detection of CEA was realized by the use of an uncharged peptide nucleic acid (PNA) probe labeled with ferrocene (Fc) as the electroactive indicator on a negatively charged indium tin oxide (ITO) electrode. In the presence of a target protein and two DNA-labeled antibodies, the proximate complex formed in homogeneous solution could unfold the molecular beacon, and a part of the unfolded molecular beacon as a primer hybridized with the RCA template to initiate the RCA process...
October 20, 2017: Analyst
https://www.readbyqxmd.com/read/29049164/electrocardiogram-in-patients-with-acute-inferior-myocardial-infarction-due-to-occlusion-of-circumflex-artery
#19
Qun Li, De-Zhao Wang, Bu-Xing Chen
To investigate the diagnostic value of electrocardiographic (ECG) ST-segment in acute inferior myocardial infarction (AIMI) caused by the left circumflex branch (LCX).A total of 240 clinical cases with AIMI in our hospital were retrospectively analyzed. All of them had received percutaneous coronary intervention (PCI) within 12 hours after symptom onset. The clinical features, ECG manifestations, and coronary artery lesion characteristics of the patients were collected.The right coronary artery (RCA) was shown to be the infarct-related artery (IRA) in 177 patients, while LCX was responsible for AIMI in 63 cases...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29046758/coronary-artery-ectasia-presenting-with-acute-inferior-wall-myocardial-infarction-in-a-young-adult
#20
Htun Latt, Sammy Aung, Kyaw Kyaw, Richard Seher
Coronary artery ectasia (CAE), a variant of coronary artery anomalies, is a rare clinical entity. Although atherosclerotic coronary artery disease (CAD) is the most common cause of acute coronary syndrome (ACS), CAE also conveys a relatively high risk for potentially life-threatening cardiac events. We report a case of a 35-year-old male with two-vessel CAE, who presented with acute inferior wall ST election myocardial infarction (STEMI). After initiating medical therapy including bivalirudin, emergent percutaneous coronary intervention (PCI) with stenting of postero-lateral branch of right coronary artery (RCA) was performed...
October 2017: Journal of Community Hospital Internal Medicine Perspectives
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