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https://www.readbyqxmd.com/read/28230577/suicide-and-suicide-attempts-on-hospital-grounds-and-clinic-areas
#1
Peter D Mills, Bradley V Watts, Robin R Hemphill
OBJECTIVES: The goal of this study was to describe suicide and suicide attempts that occurred while the patient was on hospital grounds, common spaces, and clinic areas using root cause analysis (RCA) reports of these events in a national health care organization in the United States. METHOD: This is an observational review of all RCA reports of suicide and suicide attempts on hospital grounds, common spaces, and clinic areas in our system between December 1, 1999, and December 31, 2014...
February 22, 2017: Journal of Patient Safety
https://www.readbyqxmd.com/read/28219564/coronary-artery-fistulae-involving-both-right-and-left-coronary-arteries
#2
Ahmet Bolukcu, Ahmet Can Topcu, Yavuz Sensoz, Ilyas Kayacioglu
A coronary artery fistula is defined as an abnormal direct communication between any coronary artery and any of the cardiac chambers, superior vena cava, coronary sinus, pulmonary artery, and pulmonary veins. The right coronary artery (RCA) is the most common site of origin, and right heart chambers are the most common site of drainage. However, there are few cases reported in the literature in which the fistulae originate from both the right coronary and the left anterior descending arteries and drain into the pulmonary artery...
March 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28219057/preventing-continuous-renal-replacement-therapy-induced-hypophosphatemia-an-extended-clinical-experience-with-a-phosphate-containing-solution-in-the-setting-of-regional-citrate-anticoagulation
#3
Valentina Pistolesi, Laura Zeppilli, Francesca Polistena, Maria Itala Sacco, Alessandro Pierucci, Luigi Tritapepe, Giuseppe Regolisti, Enrico Fiaccadori, Santo Morabito
AIMS: To evaluate the efficacy and safety of a commercially available phosphate-containing solution for continuous renal replacement therapy (CRRT) in preventing CRRT-related hypophosphatemia. METHODS: In heart surgery patients undergoing continuous veno-venous haemodiafiltration (CVVHDF) with regional citrate anticoagulation (RCA), we combined an 18 mmol/l citrate solution with a phosphate-containing (1.2 mmol/l) dialysate/replacement fluid evaluating the incidence of hypophosphatemia and the need for parenteral phosphorus supplementation...
February 21, 2017: Blood Purification
https://www.readbyqxmd.com/read/28217690/iatrogenic-acute-ascending-aortic-dissection-with-intramural-hematoma-during-coronary-artery-stenting-a-case-report
#4
Mohamad El-Haress, Hicham Daadaa, Shima Shahjouei, Firas El-Bitar, Hisham Bahmad
BACKGROUND: Iatrogenic acute ascending aortic dissection during percutaneous coronary intervention (PCI) is an exceptionally rare and life-threatening sequel that requires early and accurate diagnosis along with rapid management. No guidelines have yet been established to direct decisions on the different treatment options that can be employed in the setting of acute aortic dissections caused by PCI. However, similar cases have been treated either by intracoronary stenting and conservative management as in localized aortocoronary dissections or by surgical intervention in cases of extensive aortic dissections...
2017: Frontiers in Surgery
https://www.readbyqxmd.com/read/28214964/speckle-tracking-in-alcapa-patients-after-surgical-repair-as-predictor-of-residual-coronary-disease
#5
Biagio Castaldi, Vladimiro Vida, Elena Reffo, Massimo Padalino, Queenette Daniels, Giovanni Stellin, Ornella Milanesi
Surgical re-implantation of the left coronary artery (LCA) is the treatment of choice in anomalous origin of left coronary artery from pulmonary artery (ALCAPA). Despite normalization of left ventricular function after surgery, residual coronary lesions or myocardial fibrosis may be found. The aim of this study was to detect regional left ventricular dysfunction predictive of coronary lesions or residual myocardial fibrosis using speckle tracking. We enrolled ten patients treated with surgical re-implantation of LCA for ALCAPA...
February 18, 2017: Pediatric Cardiology
https://www.readbyqxmd.com/read/28194952/-off-to-on-surface-enhanced-raman-spectroscopy-platform-with-padlock-probe-based-exponential-rolling-circle-amplification-for-ultrasensitive-detection-of-microrna-155
#6
Yi He, Xia Yang, Ruo Yuan, Yaqin Chai
In this work, an "off" to "on" surface-enhanced Raman spectroscopy (SERS) platform was constructed for ultrasensitive detection of microRNA (miRNA) by using a magnetic SERS substrate (Co@C/PEI/Ag) and padlock probe-based exponential rolling circle amplification (P-ERCA) strategy. Herein, miRNA 155 could act as primers to initiate rolling circle amplification (RCA) for producing a long repeat sequence, and then the obtained DNA would be cleaved into two kinds of single-stranded DNAs in the presence of nickase...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28188374/complete-genome-sequence-of-bean-leaf-crumple-virus-a-novel-begomovirus-infecting-common-bean-in-colombia
#7
Monica Carvajal-Yepes, Leidy Zambrano, Juan M Bueno, Bodo Raatz, Wilmer J Cuellar
A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoamerican beans. Such symptoms have been reported increasingly in Colombia since at least 2002, and we detected the virus in leaf material collected since 2008. Sequence analysis showed that the virus is a member of a distinct species, sharing 81% and 76% nucleotide (nt) sequence identity (in DNA-A and DNA-B, respectively) to other begomoviruses infecting common bean in the Americas...
February 10, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28179971/atypical-double-right-coronary-artery-presenting-with-acute-coronary-syndrome-cardiogenic-shock-and-complete-heart-block
#8
Santosh Kumar Sinha, Shravan Singh, Vikash Chaturvedi, Puneet Agrawal, Mahmadula Razi, Anupam Mahrotra, Vikas Mishra, Mukesh Kumar, Nasar Abdali, Dibbendhu Khanra, Ramesh Thakur, Chandra Mohan Varma, Umeshwar Pandey
Double right coronary artery (RCA) is an extremely rare coronary artery anomaly. We here report an atherosclerotic double RCA which appeared after primary percutaneous intervention performed to treat a 34-year-old male presenting with acute inferior myocardial infarction, cardiogenic shock and complete heart block. This is an unusual case as double RCA had been hidden by total atherosclerotic occlusion of the proximal part of the RCA and complete restoration of patency led complete heart block back to normal sinus rhythm...
March 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/28168688/repeat-coronary-artery-dissection-in-pregnancy-a-case-report-and-review-of-the-literature
#9
Casey P Bitting, Ross E Zumwalt
Non-atherosclerotic spontaneous coronary artery dissection (NA-SCAD) is a rare cause of morbidity and mortality with a propensity for young, healthy, and often peripartum women. NA-SCAD etiology is poorly understood, with possible hormonal and hereditary mechanisms. Current treatment strategies range from conservative management (often showing resolution on angiographic follow-up) to invasive angiographic procedures. Rarely, NA-SCAD has recurred in another coronary artery, ranging hours to years later. We report NA-SCAD of the right coronary artery (RCA) in a 30-year old, 3-month postpartum female with an additional autopsy finding of remote myocardial infarction (MI) in the left anterior descending (LAD) coronary artery territory...
February 7, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28166424/lectin-histochemistry-for-detecting-cadmium-induced-changes-in-the-glycosylation-pattern-of-rat-placenta
#10
M C Díaz, N V González, C N Zanuzzi, R Najle, C G Barbeito
Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we investigated changes in its glycosylated components in Cd treated dams at days 4, 7, 10 and 15 of gestation using lectin histochemistry. CdCl2 was administered to pregnant rats; control animals received sterile normal saline. Placentas were processed for DBA, Con A, SBA, PNA, UEA-I, RCA-I and WGA lectin histochemistry to evaluate changes in the carbohydrate pattern of the placenta that might modify cell interactions and contribute to embryonic alterations...
February 6, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28162251/reliability-and-utility-of-citizen-science-reef-monitoring-data-collected-by-reef-check-australia-2002-2015
#11
Terence Done, Chris Roelfsema, Andrew Harvey, Laura Schuller, Jocelyn Hill, Marie-Lise Schläppy, Alexandra Lea, Anne Bauer-Civiello, Jennifer Loder
Reef Check Australia (RCA) has collected data on benthic composition and cover at >70 sites along >1000km of Australia's Queensland coast from 2002 to 2015. This paper quantifies the accuracy, precision and power of RCA benthic composition data, to guide its application and interpretation. A simulation study established that the inherent accuracy of the Reef Check point sampling protocol is high (<±7% error absolute), in the range of estimates of benthic cover from 1% to 50%. A field study at three reef sites indicated that, despite minor observer- and deployment-related biases, the protocol does reliably document moderate ecological changes in coral communities...
February 2, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28159108/an-enhanced-chemiluminescence-resonance-energy-transfer-aptasensor-based-on-rolling-circle-amplification-and-ws2-nanosheet-for-staphylococcus-aureus-detection
#12
Liling Hao, Huajie Gu, Nuo Duan, Shijia Wu, Xiaoyuan Ma, Yu Xia, Zui Tao, Zhouping Wang
A chemiluminescence resonance energy transfer aptasensor was fabricated for the detection of Staphylococcus aureus (S. aureus) with Co(2+) enhanced N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (Co(2+)/ABEI-AuNFs) as donor and WS2 nanosheet as acceptor. In the presence of S. aureus, rolling circle amplification (RCA) can be started. Partially complementary sequence of RCA product functional ABEI-AuNFs (cDNA-ABEI-AuNFs) were then annealed to multiple sites of the RCA product to form duplex complex...
March 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28155686/hemodynamic-analysis-of-sequential-graft-from-right-coronary-system-to-left-coronary-system
#13
Wenxin Wang, Boyan Mao, Haoran Wang, Xueying Geng, Xi Zhao, Huixia Zhang, Jinsheng Xie, Zhou Zhao, Bo Lian, Youjun Liu
BACKGROUND: Sequential and single grafting are two surgical procedures of coronary artery bypass grafting. However, it remains unclear if the sequential graft can be used between the right and left coronary artery system. The purpose of this paper is to clarify the possibility of right coronary artery system anastomosis to left coronary system. METHODS: A patient-specific 3D model was first reconstructed based on coronary computed tomography angiography (CCTA) images...
December 28, 2016: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28154267/contrast-induced-hyperemia-as-an-alternative-to-drug-induced-hyperemia-in-the-evaluation-of-the-fractional-flow-reserve-in-coronary-lesions
#14
Nobuo Shiode, Tomokazu Okimoto, Hiromichi Tamekiyo, Tomoharu Kawase, Kenichi Yamane, Yuzo Kagawa, Yuto Fujii, Yusuke Ueda, Naoya Hironobe, Yasuko Kato, Yasuhiko Hayashi
Objective Measuring the fractional flow reserve (FFR) requires the induction of coronary hyperemia, usually with adenosine, adenosine triphosphate (ATP), or papaverine. However, adenosine can induce rhythmic complications, and intracoronary boluses of papaverine that prolong the QT interval can cause ventricular tachycardia. Injection of contrast media, which is routinely performed to validate the FFR guidewire placement, also induces hyperemia and may be an alternative method of measuring the FFR. We evaluated the diagnostic accuracy of the FFR after contrast hyperemia (FFRcont) compared to FFR evaluated after intracoronary papaverine (FFRpp)...
2017: Internal Medicine
https://www.readbyqxmd.com/read/28150085/influence-of-scan-technique-on-intracoronary-transluminal-attenuation-gradient-in-coronary-ct-angiography-using-128-slice-dual-source-ct-multi-beat-versus-one-beat-scan
#15
Hae Jin Kim, Sung Mok Kim, Jin-Ho Choi, Yeon Hyeon Choe
The purpose of our study was to investigate the impact of temporal uniformity and adjustment by the contrast opacification enhancement in the aorta on the performance of transluminal attenuation gradient (TAG) for obstructive coronary artery disease. A total of 274 coronary arteries from 94 patients who underwent both multi- and single-beat scan using 128-slice scanner at the same time were enrolled. TAG and corrected coronary opacification (CCO) of both scan technique were compared against obstructive coronary arteries defined by diameter stenosis ≥50%...
February 1, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28142225/ecotype-diversification-of-an-abundant-roseobacter-lineage
#16
Ying Sun, Yao Zhang, James T Hollibaugh, Haiwei Luo
The Roseobacter DC5-80-3 cluster (also known as the RCA clade) is among the most abundant bacterial lineages in temperate and polar oceans. Previous studies revealed two phylotypes within this cluster that are distinctly distributed in the Antarctic and other ocean provinces. Here, we report a nearly complete genome co-assembly of three closely related single cells co-occurring in the Antarctic, and compare it to the available genomes of the other phylotype from ocean regions where iron is more accessible but phosphorus and nitrogen are less...
January 31, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28142124/quantitative-real-time-monitoring-of-rca-amplification-of-cancer-biomarkers-mediated-by-a-flexible-ion-sensitive-platform
#17
Bruno Veigas, Joana Pinto, Raquel Vinhas, Tomás Calmeiro, Rodrigo Martins, Elvira Fortunato, Pedro Viana Baptista
Ion sensitive field-effect transistors (ISFET) are the basis of radical new sensing approaches. Reliable molecular characterization of specific detection of DNA and/or RNA is vital for disease diagnostics and to follow up alterations in gene expression profiles. Devices and strategies for biomolecular recognition and detection should be developed into reliable and inexpensive platforms. Here, we describe the development of a flexible thin-film sensor for label free gene expression analysis. A charge modulated ISFET based sensor was integrated with real-time DNA/RNA isothermal nucleic acid amplification: Loop-mediated isothermal amplification (LAMP) and Rolling Circle Amplification (RCA) techniques for c-MYC and BCR-ABL1 genes, allowing for the real-time quantification of template...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28137454/residual-activity-correction-in-quantitative-myocardial-perfusion-13-n-ammonia-pet-imaging-a-study-in-post-mi-patients
#18
George Markousis-Mavrogenis, Luis Eduardo Juárez-Orozco, Erick Alexanderson
BACKGROUND/INTRODUCTION/AIM: Positron Emission Tomography (PET) is the gold standard for quantification of Myocardial Blood Flow (MBF). A standard PET scan is acquired in two phases (rest and pharmacological stress). (13)N-ammonia is a perfusion radiotracer that may show residual activity which may affect MBF estimation during the second phase of the scan. An algorithm for Residual Activity Correction (RAC) is available when reconstruction is performed using Syngo MBF (by Siemens). The aim of this study was to evaluate differentes in MBF estimation with and without RAC by Syngo MBF in patients with a previous MI using (13)N-ammonia PET...
January 27, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/28112295/a-dna-directed-covalent-conjugation-fluorescence-probe-for-in-vitro-detection-of-functional-matrix-metalloproteinases
#19
Nan Li, Linglu Yi, Ziyi He, Weifei Zhang, Haifang Li, Jin-Ming Lin
Matrix metalloproteinases (MMPs) have been considered to contribute to the progression of tumorigenesis and tumor invasion; MMP-9 in particular, has been regarded as a priority target in cancer treatment due to its massive up-regulation in malignant tissues and its ability to degrade type IV collagen. In this work, we employed a DNA-directed covalent conjugation method to design a fluorescence probe for in vitro detection of functional matrix metalloproteinases, by which a nitrilotriacetic acid (NTA)-modified DNA probe can combine with the Zn(2+) in the active site of MMPs, and then a molecule beacon (MB) modified FITC and BHQ1 can open to bond with their complementary base, NTA-modified DNA...
January 23, 2017: Analyst
https://www.readbyqxmd.com/read/28108702/is-rectal-douching-and-sharing-douching-equipment-associated-with-anorectal-chlamydia-and-gonorrhoea-a-cross-sectional-study-among-men-who-have-sex-with-men
#20
Rca Achterbergh, J J van der Helm, W van den Boom, T Heijman, I G Stolte, M S van Rooijen, Hjc de Vries
INTRODUCTION: Men who have sex with men (MSM) are at high risk for anorectal chlamydia and gonorrhoea infections. Many MSM use rectal douches in preparation for sex, which might break down the mucosal barrier function and facilitate the acquisition of STI. We determined whether rectal douching or sharing douching equipment was associated with anorectal chlamydia and gonorrhoea. METHODS: In a cross-sectional study among 994 MSM attending the STI outpatient clinic of Amsterdam between February and April 2011, data were collected on rectal douching, sexual behaviour and STI...
January 20, 2017: Sexually Transmitted Infections
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