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Mohammed El-Amin Said, Marcello Militello, Sergio Saia, Luca Settanni, Aurora Aleo, Caterina Mammina, Isabelle Bombarda, Pierre Vanloot, Christian Roussel, Nathalie Dupuy
Aerial parts of Artemisia arborescens were collected from different sites of the Mediterranean area (southwestern Algeria and southern Italy) and the chemical composition of their essential oil (EO) extracted by hydrodistillation was studied by both gas chromatography (GC) equipped with an enantioselective capillary column and GC/mass spectrometry (GC/MS). The EOs obtained were tested against several Listeria monocytogenes strains. Using GC and GC/MS, 41 compounds were identified, accounting for 96.0 - 98...
August 2016: Chemistry & Biodiversity
Marija Nenadić, Marina Soković, Jasmina Glamočlija, Ana Ćirić, Vesna Perić-Mataruga, Larisa Ilijin, Vele Tešević, Ljubodrag Vujisić, Marina Todosijević, Nikola Vesović, Srećko Ćurčić
The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens--eight bacterial both gram-positive and gram-negative species and eight fungal species...
April 2016: Die Naturwissenschaften
Laima Cesonienė, Remigijus Daubaras, Pranas Viškelis, Antanas Sarkinas
Viburnum opulus is a plant with fruits that are rich in biologically active substances, making it valuable to the food and pharmaceutical industries. Here, we present our study of the total phenolic and anthocyanin contents and antimicrobial activity in the fruit juice of six V. opulus L. accessions. The cultivar 'Krasnaya Grozd' was notable for its exceptionally large amount of total phenolics, 1168 mg/100 g, with anthocyanins comprising 3-5 % of the total phenolic content. The evaluation of the antimicrobial properties confirmed that the juice of V...
September 2012: Plant Foods for Human Nutrition
Igor Jerković, Dajana Gašo-Sokač, Hrvoje Pavlović, Zvonimir Marijanović, Mirko Gugić, Ivana Petrović, Spomenka Kovač
GC and MS were used for the analysis of Croatian Centaurium erythraea Rafn essential oil (obtained by hydrodistillation) and headspace (applying headspace solid-phase microextraction). The headspace contained numerous monoterpene hydrocarbons (the major ones were terpinene-4-ol, methone, p-cymene, γ-terpinene and limonene). Oxygenated monoterpenes were present in the headspace and oil, while 1,8-cineole, bornyl acetate and verbenone were present only in the headspace. High headspace percentages of toluene and naphthalene were found, followed by hemimellitene...
2012: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
A Berardi, L Lugli, C Rossi, M C China, G Vellani, R Contiero, F Calanca, F Camerlo, F Casula, C Di Carlo, M R Rossi, R Chiarabini, M Ferrari, S Minniti, C Venturelli, D Silvestrini, I Dodi, A Zucchini, F Ferrari
Neonatal bacterial meningitis (NM) continues to be a serious disease with an unchanging rate of adverse outcome of 20-60%, despite a worldwide decline in mortality. The 3 major pathogens in developed countries are: Group B streptococcus, gram negative rods and Lysteria monocytogenes. Signs and symptoms of NM may be subtle, unspecific, vague, atypical or absent. In order to exclude NM, all infants with proven or suspected sepsis should undergo lumbar puncture. Positive culture of cerebrospinal fluid may be the only way to diagnose NM and to identify the pathogen, as CSF parameters Smay be normal at early stages and NM may occur frequently (up to 30% of cases) in the absence of bacteraemia...
June 2010: Minerva Pediatrica
Mahanama De Zoysa, Chamilani Nikapitiya, Youngdeuk Lee, Sukkyoung Lee, Chulhong Oh, Ilson Whang, Sang-Yeop Yeo, Cheol Young Choi, Jehee Lee
The regulation of transcriptional activation is an essential and critical point in gene expression. In this study, we describe a novel transcription factor activator protein-1 (Ap-1) gene from disk abalone Haliotis discus discus (AbAp-1) for the first time in mollusk. It was identified by homology screening of an abalone normalized cDNA library. The cloned AbAp-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. The AbAp-1 gene is composed of a characteristic Jun transcription factor domain and a highly conserved basic leucine zipper (bZIP) signature similar to known Ap-1 genes...
December 2010: Fish & Shellfish Immunology
L S Buzoleva, V E Terekhova, A M Krivosheeva, N A Aĭzdaĭcher
AIM: To study the effects of sea microalgae exometabolites on reproduction of pathogenic bacteria. MATERIALS AND METHODS: Experiments were performed with unialgal cultures of sea microalgae Phaeodactylum tricornutum, Chlorella minutissima, Chroomonas salina. Strains of Lysteria monocytogenes 4b, Staphylococcus aureus, Salmonella typhimurium with typical cultural, morphologic, biochemical and antigenic characteristics were used as model bacteria. Cultivation of microalgae was performed in synthetic and natural sea water (salinity 32 per thousand)...
March 2010: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
Mahanama De Zoysa, Chamilani Nikapitiya, Yucheol Kim, Chulhong Oh, Do-Hyung Kang, Ilson Whang, Se-Jae Kim, Jae-Seong Lee, Cheol Young Choi, Jehee Lee
Here, we report the identification and characterization of allograft inflammatory factor-1 (AIF-1) from disk abalone Haliotis discus discus that was denoted as AbAIF-1. The full-length cDNA of AbAIF-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kDa molecular mass. Analysis of AbAIF-1 sequence showed that it shares characteristic two EF hand Ca(+2)-binding motifs. Results from phylogenetic analysis further confirm that AbAIF-1 is a member of the AIF-1 family similar to invertebrate and vertebrate counterparts suggesting it has high evolutional conservation...
August 2010: Fish & Shellfish Immunology
Mahanama De Zoysa, Chamilani Nikapitiya, Chulhong Oh, Ilson Whang, Jae-Seong Lee, Sung-Ju Jung, Cheol Young Choi, Jehee Lee
The lipopolysaccharide-induced TNF-alpha factor (LITAF) and Rel family nuclear factor kappaB (Rel/NF-kB) are two important transcription factors which play major roles in the regulating inflammatory cytokine, apoptosis and immune related genes. Here, we report the discovery of disk abalone LITAF (AbLITAF) and Rel/NF-kB (AbRel/NF-kB) homologues and their immune responses. Full-length cDNA of AbLITAF consists of 441 bp open reading frame (ORF) that translates into putative peptide of 147 aa. Analysis of AbLITAF sequence showed it has characteristic LITAF (Zn(+2)) binding domain with two CXXC motifs...
May 2010: Fish & Shellfish Immunology
Mahanama De Zoysa, Ilson Whang, Youngdeuk Lee, Sukkyoung Lee, Jae-Seong Lee, Jehee Lee
Gene-encoded antimicrobial peptides (AMPs) serve a major role in host defense systems against pathogens. In this study, cDNA of a new mollusk defensin was identified from a normalized cDNA library constructed from whole tissues of disk abalone. Abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. The presence of an invertebrate defensin family domain, an arrangement of six cysteine residues and their disulfide linkage in C(1)-C(4), C(2)-C(5) and C(3)-C(6) form, an alpha helix in three-dimensional structure and a phylogenetic relationship suggests that abalone defensin could be a new member of the invertebrate defensin family, and related to arthropod defensins...
February 2010: Fish & Shellfish Immunology
S M Omarova, R I Isaeva, E M Akhmedova, P M Murtuzalieva
Isolation of Lysteria monocytogenes from clinical specimens in obstetric-gynecological pathology is often difficult owing to the presence of the mixed microbial flora. Results of study of new Russian growth media for isolation of Listeria for microbiological diagnostics of listeriosis in pregnancy are presented. They show diagnostic efficacy of the developed media for laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology.
November 2008: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
Mahanama De Zoysa, Sungju Jung, Jehee Lee
Tumor necrosis factor alpha (TNF-alpha) is considered as a multifunctional immune modulator that plays an important role in the innate and adaptive immune systems in vertebrates. Here, we described the characterization and expression analysis of the first TNF-alpha homologue in mollusk abalone, named as AbTNF-alpha. It has 930-bp full length with a 717-bp open reading frame (ORF), encoding 239 amino acids. The AbTNF-alpha amino acid sequence shows the characteristic TNF family signature, N-terminal transmembrane domain consisting of a hydrophobic amino acid cluster and cell attachment sequence at (155)RGD(157)...
April 2009: Fish & Shellfish Immunology
Vanja M Tadić, Silva Dobrić, Goran M Marković, Sofija M Dordević, Ivana A Arsić, Nebojsa R Menković, Tanja Stević
Hawthorn [Crataegus monogyna Jacq. and Crataegus oxyacantha L.; sin. Crataegus laevigata (Poiret) DC., Rosaceae] leaves, flowers, and berries are used in traditional medicine in the treatment of chronic heart failure, high blood pressure, arrhythmia, and various digestive ailments, as well as geriatric and antiarteriosclerosis remedies. According to European Pharmacopoeia 6.0, hawthorn berries consist of the dried false fruits of these two species or their mixture. The present study was carried out to test free-radical-scavenging, anti-inflammatory, gastroprotective, and antimicrobial activities of hawthorn berries ethanol extract...
September 10, 2008: Journal of Agricultural and Food Chemistry
Naomi Courtemanche, Doug Barrick
Although the folding of alpha-helical repeat proteins has been well characterized, much less is known about the folding of repeat proteins containing beta-sheets. Here we investigate the folding thermodynamics and kinetics of the leucine-rich repeat (LRR) domain of Internalin B (InlB), an extracellular virulence factor from the bacterium Lysteria monocytogenes. This domain contains seven tandem leucine-rich repeats, of which each contribute a single beta-strand that forms a continuous beta-sheet with neighboring repeats, and an N-terminal alpha-helical capping motif...
January 2008: Protein Science: a Publication of the Protein Society
Moisés Armides Franco-Molina, Edgar Mendoza-Gamboa, Paloma Castillo-Tello, Reyes S Tamez-Guerra, Licet Villarreal-Treviño, Rolando Tijerina-Menchaca, Leonardo Castillo-León, Pablo Zapata-Benavides, Cristina Rodríguez-Padilla
The rapidly developing resistance of many infectious pathogenic organisms to modern drugs has spurred scientists to search for new sources of antibacterial compounds. One potential candidate, bDLE (dialysis at 10 to 12 kDa cut-off) and its fractions ("S" and "L" by 3.5 kDa cut-off and I, II, III, and IV by molecular exclusion chromatography), was evaluated for antibacterial activity against pathogenic bacterial strains (Staphylococcus aureus, Streptococcus pyogenes, Lysteria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) using standard antimicrobial assays...
2006: Immunopharmacology and Immunotoxicology
N Cioffi, N Ditaranto, L Torsi, R A Picca, L Sabbatini, A Valentini, L Novello, G Tantillo, T Bleve-Zacheo, P G Zambonin
Copper-fluoropolymer (Cu-CFx) nano-composite films are deposited by dual ion-beam sputtering. The extensive analytical characterization of these layers reveals that inorganic nanoparticles composed of Cu(II) species are evenly dispersed in a branched fluoropolymer matrix. In particular, X-ray photoelectron spectroscopy has been employed to study the surface chemical composition of the material and to assess how it changes on increasing the copper loading in the composite. Transmission electron microscopy reveals that the copper nanoclusters have a mean diameter of 2-3 nm and are homogeneously in-plane distributed in the composite films...
February 2005: Analytical and Bioanalytical Chemistry
E Adorisio, D D Tassone, A Conte, L Sebastiani Annicchiarico
Animal food-stuffs are known to be potential vehicles of Listeria monocytogenes. The contamination can be caused from processing or enviromental sources and from infected animals. This hypothesis has been checked in the present work. The authors found that 13.2% of 189 swines were carriers of Listeria monocytogenes, the microrganism was isolated from salivary glands, mesenterial gangles and tonsils. The authors suggest some preventive intervention to reduce both the environmental circulation of Listeria monocytogenes and the human risk of infection...
September 2003: Annali di Igiene: Medicina Preventiva e di Comunità
A Abolmaaty, C Vu, J Oliver, R E Levin
A new lysis solution designated TZ, consisting of 2.0% Triton X-100 plus 2.5 mg sodium azide/ml in 0.1 M Tris-HCl buffer at pH 8.0, yielded higher levels of genomic DNA from Escherichia coli O157:H7 cells compared with a number of other commonly used cell lysis methods. Ethidium bromide stained DNA bands resulting from PCR amplification of target DNA from 100 CFU of E. coli O157:H7 were readily detected following electrophoresis of agarose gels. In contrast, conventional cell lysis methods failed to detect target DNA from 100 CFU after PCR amplification...
2000: Microbios
H Villegas Castrejon, M Morales Juarez, O L Peralta, S Nájera Ruano, E Flores Rivera
Tracheal epithelial damage, was evaluated in prematures that where intubated and with mechanical ventilation; biopsies were taken in seven preterm neonates; weight was 1100 to 2350; five were by cesarean section, y one via vaginal. Bronchial culture was negative in five, in one Lysteria monocitogena, and in another one Pseudomonas spiralis; biopsy was taken at different times o intubation. Five died because of pneumonia associated to other diseases. In optical and ultrastructural study it was found that with one day of intubation there was cilia loss; with three days of ventilation there was ciliary cells loss, and not ciliary cellular death and there were no cellular unions...
May 1997: Ginecología y Obstetricia de México
V Chiaradia, A Pomes, P De Paoli, A M Minca, G Santini
We herein describe the case of Lysteria monocytogenes meningitis. The isolation and identification of the bacteria causing the infection ollowed the antibiotic treatment followed by complete remission. Our data also support the hypothesis on intense immunological response producing a favourable evolution of the disease.
January 1985: Rivista di Neurologia
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