Qi Chen, Meixian Jin, Simin Wang, Kexin Wang, Liqin Chen, Xiaojuan Zhu, Ying Zhang, Yi Wang, Yang Li, Shao Li, Youmin Zeng, Lei Feng, Wanren Yang, Yi Gao, Shuqin Zhou, Qing Peng
Acute liver failure (ALF) is characterized by rapid liver cell destruction. It is a multi-etiological and fulminant complication with a clinical mortality of over 80%. Therapy using mesenchymal stem cells (MSCs) or MSCs-derived exosomes can alleviate acute liver injury, which has been demonstrated in animal experiments and clinical application. However, similar to other stem cells, different cell sources, poor stability, cell senescence and other factors limit the clinical application of MSCs. To achieve mass production and quality control on stem cells and their exosomes, transfecting umbilical cord mesenchymal stem cell (UCMSC) with lentivirus overexpressing human telomerase reverse transcriptase (hTERT) gene, the hTERT-UCMSC was constructed as an immortalized MSC cell line...
2023: Journal of Tissue Engineering