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https://www.readbyqxmd.com/read/28636136/capillary-zone-electrophorsis-for-the-analysis-of-naturally-occurring-2-hydroxycitric-acids-and-their-lactones
#1
B L Abhijith, Moolya Mohan, David Joseph, Simimole Haleema, Hassan Y Aboul-Enein, Ibrahim Ibnusaud
A simple capillary zone electrophoresis method with direct ultraviolet detection has been developed for the analysis of naturally occurring diastereomeric 2-hydroxycitric acid lactones. Using 50 mM sodium phosphate buffer of pH 7, a baseline resolution Rs > 3.0 was observed for all organic acids selected for the present study. This method was employed for the quantitative determination of title acids present in the plant sources namely G. cambogia fruit rinds and H. sabdariffa calyx. Conversion of 2-hydroxycitric acids to their lactones on heating above plant sources is deliberated...
June 21, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28635997/a-spin-column-free-approach-to-sodium-hydroxide-based-glycan-permethylation
#2
Yueming Hu, Chad R Borges
Glycan permethylation was introduced as a tool to facilitate the study of glycans in 1903. Since that time, permethylation procedures have been continually modified to improve permethylation efficiency and qualitative applicability. Typically, however, either laborious preparation steps or cumbersome and uneconomical spin columns have been needed to obtain decent permethylation yields on small glycan samples. Here we describe a spin column-free (SCF) glycan permethylation procedure that is applicable to both O- and N-linked glycans and can be employed upstream to intact glycan analysis by MALDI-MS, ESI-MS, or glycan linkage analysis by GC-MS...
June 21, 2017: Analyst
https://www.readbyqxmd.com/read/28634326/a-chrysoporthe-cubensis-enzyme-cocktail-produced-from-a-low-cost-carbon-source-with-high-biomass-hydrolysis-efficiency
#3
Thiago Rodrigues Dutra, Valéria Monteze Guimarães, Ednilson Mascarenhas Varela, Lílian da Silva Fialho, Adriane Maria Ferreira Milagres, Daniel Luciano Falkoski, José Cola Zanuncio, Sebastião Tavares de Rezende
Low cost and high efficiency cellulolytic cocktails can consolidate lignocellulosic ethanol technologies. Sugarcane bagasse (SCB) is a low cost agro-industrial residue, and its use as a carbon source can reduce the costs of fungi cultivation for enzyme production. Chrysoporthe cubensis grown under solid state fermentation (SSF) with wheat bran has potential to produce efficient enzymatic extracts for SCB saccharification. This fungus was grown under submersed fermentation (SmF) and SSF with in natura SCB, pretreated with acid or alkali and with others carbon sources...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28634114/peanut-digestome-identification-of-digestion-resistant-ige-binding-peptides
#4
Luigia Di Stasio, Gianluca Picariello, Mariantonietta Mongiello, Rita Nocerino, Roberto Berni Canani, Simona Bavaro, Linda Monaci, Pasquale Ferranti, Gianfranco Mamone
Stability to proteolytic degradation in the digestive tract is considered a general feature shared by most food allergens. Current digestibility models exclusively utilize purified allergen proteins, neglecting the relevant effects of matrix that occur for foodstuff systems. In the present study, we investigated digestion stability of the major peanut allergens directly in the natural matrix using an in vitro static model that simulates the gastrointestinal digestion including the oral, gastric, duodenal and intestinal (brush border membrane enzymes) phases...
June 17, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28634057/evaluation-of-the-biotechnological-potential-of-a-novel-purified-protease-bs1-from-bacillus-safensis-s406-on-the-chitin-extraction-and-detergent-formulation
#5
Samiha Mhamdi, Intidhar Bkhairia, Rim Nasri, Tahar Mechichi, Moncef Nasri, Alya Sellami Kamoun
The aim of this study is the production of High-Added Value enzyme for Industrial Uses. An extracellular alkaline stable protease BS1 was purified from a newly bacterium, Bacillus safensis S406, isolated from the Sfax Solar Saltern. BS1 was purified by a process involving sulfate d'ammonium sulfate precipitation, sephadex G-75, Mono-Q anion-exchange chromatography and ultrafiltation. The enzyme has a molecular weight of 24kDa and appeared as a single band on native-PAGE. The optimum pH and temperature values of its proteolytic activity were pH 11...
June 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28633930/phospholipase-a2-in-the-venom-of-three-cottonmouth-snake-species
#6
Ying Jia, Boris Ermolinsky, Aryana Garza, Daniele Provenzano
Two phospholipase A2s (PLA2s), Asp49 (D49) and Lys49 (K49), were purified by one-step reverse-phase high performance liquid chromatography (RP-HPLC) from the venom of each of the three subspecies of cottonmouth snake, Western cottonmouth (Agkistrodon piscivorus leucostoma; Apl), Eastern cottonmouth (Agkistrodon piscivorus piscivorus; App) and Florida cottonmouth (Agkistrodon piscivorus conanti; Apc). Venom protein profiles and PLA2s elution pattern of the three cottonmouth snakes were remarkably similar displaying four similar sharp and two wide peaks; in all cases K49 PLA2 eluted first followed by D49 PLA2...
June 17, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28633895/synthesis-of-meon-neoglycosides-of-digoxigenin-with-6-deoxy-and-2-6-dideoxy-d-glucose-derivatives-and-their-anticancer-activity
#7
Dong-Dong Wang, Xiao-San Li, Yu-Zhou Bao, Jie Liu, Xiao-Kun Zhang, Xin-Sheng Yao, Xue-Long Sun, Jin-Shan Tang
Cardiac glycosides show anticancer activities and their deoxy-sugar chains are vital for their anticancer effects. In order to study the structure-activity relationship (SAR) of cardiac glycosides toward cancers and get more potent anticancer agents, a series of MeON-neoglycosides of digoxigenin was synthesized and evaluated. First, ten 6-deoxy- and 2,6-dideoxy-d-glucopyranosyl donors were synthesized starting from methyl α-d-glucopyranoside and 2-deoxy-d-glucose. Meanwhile, the digoxigenin was obtained by acidic hydrolysis of commercially available digoxin as glycosyl acceptor...
June 3, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28633842/effects-of-multi-frequency-ultrasound-pretreatment-under-low-power-density-on-the-enzymolysis-and-the-structure-characterization-of-defatted-wheat-germ-protein
#8
Xue Yang, Yunliang Li, Suyun Li, Ayobami Olayemi Oladejo, Yucheng Wang, Shanfen Huang, Cunshan Zhou, Yang Wang, Li Mao, Yanyan Zhang, Haile Ma, Xiaofei Ye
The effects of ultrasonic frequency mode, power density, pretreatment time and other parameters under low power density on the degree of hydrolysis (DH) of defatted wheat germ protein (DWGP) and angiotensin-I-converting enzyme (ACE) inhibitory activity of DWGP hydrolysate were studied in this research. Ultraviolet-visible (UV-Vis) spectra, free sulfhydryl (SH), disulfide bond (SS), surface hydrophobicity and hydrophobic protein content of ultrasound-pretreated protein and hydrophobic amino acid (HAA) content of alcalase-hydrolysate of DWGP were measured under optimized ultrasonic condition...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633819/effects-of-ultrasound-pretreatment-with-different-frequencies-and-working-modes-on-the-enzymolysis-and-the-structure-characterization-of-rice-protein
#9
Xue Yang, Yunliang Li, Suyun Li, Ayobami Olayemi Oladejo, Siyu Ruan, Yucheng Wang, Shanfen Huang, Haile Ma
The effects of ultrasound pretreatment with different frequencies and working modes, including mono-frequency ultrasound (MFU), dual-frequency ultrasound (DFU) and tri-frequency ultrasound (TFU), on the degree of hydrolysis (DH) of rice protein (RP) and angiotensin-I-converting enzyme (ACE) inhibitory activity of RP hydrolysate were investigated. Ultraviolet-visible (UV) spectroscopy, fourier transform infrared (FTIR) spectroscopy, surface hydrophobicity and scanning electron microscopy (SEM) of RP pretreated with ultrasound were measured...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633114/do-glucosinolate-hydrolysis-products-reduce-nitrous-oxide-emissions-from-urine-affected-soil
#10
S F Balvert, J Luo, L A Schipper
New Zealand agriculture is predominantly comprised of pastoral grazing systems and deposition of animal excreta during grazing has been identified as a major source of nitrous oxide (N2O) emissions. Nitrification inhibitors have been shown to significantly reduce nitrous oxide emissions from grazing pastoral systems, and some plants have been identified as having nitrification inhibiting properties. Brassica crops are one such example as they contain the secondary metabolite glucosinolate (GLS) whose hydrolysis products are thought to slow soil nitrogen cycling...
June 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28631838/aging-of-zn-added-to-soils-with-a-wide-range-of-different-properties-factors-and-modeling
#11
Xi Zhang, Bao Jiang, Yibing Ma
The aging of metals added to soil is significant in ecological risk assessment and the derivation of soil environmental quality criteria. Semi-mechanistic aging models have been developed for some metals, but not for zinc (Zn). In the present study, a published dataset detailing the changes in isotopic exchangeability with time, after water soluble Zn was added to 23 soils and incubated for 813 d, was used to analyze the effect of soil properties on aging and develop empirical and mechanistic models. Aging was mainly controlled by soil pH, probably due to precipitation, and was linearly correlated with the square root of incubation time, probably due to diffusion...
June 20, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28631814/germination-assisted-enzymatic-hydrolysis-can-improve-the-quality-of-soybean-protein
#12
Hui Yang, Xin Li, Jinyan Gao, Ping Tong, Anshu Yang, Hongbing Chen
This study aimed to investigate the effects of combined germination and Alcalase hydrolysis on the quality of soybean protein. Protein profiles, water solubility, foaming and emulsifying properties, thixotropic properties, and in vitro protein digestibility (IVPD) were tested, the chemical score (CS), essential amino acid index (EAAI), and protein efficiency ratio (PER) of soybean protein were also defined. The combined treatment of germination and Alcalase hydrolysis remarkably improved the solubility, emulsification activity index, emulsion stability index, and foaming capacity of soybean protein...
June 20, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28631488/synthesis-of-6-7-dideoxysqualestatin-h5-by-carbonyl-ylide-cycloaddition-rearrangement-and-cross-electrophile-coupling
#13
Younes Fegheh-Hassanpour, Tanzeel Arif, Herman O Sintim, Hamad H Al Mamari, David M Hodgson
An asymmetric synthesis of (-)-6,7-dideoxysqualestatin H5 is reported. Key features of the synthesis include the following: (1) highly diastereoselective n-alkylation of a tartrate acetonide enolate and subsequent oxidation-hydrolysis to provide an asymmetric entry to a β-hydroxy-α-ketoester motif; (2) facilitation of Rh(II)-catalyzed cyclic carbonyl ylide formation-cycloaddition by co-generation of keto and diazo functionality through ozonolysis of an unsaturated hydrazone; and (3) stereoretentive Ni-catalyzed Csp(3)-Csp(2) cross-electrophile coupling between tricarboxylate core and unsaturated side chain to complete the natural product...
June 20, 2017: Organic Letters
https://www.readbyqxmd.com/read/28630961/a-biocatalytic-and-thermoreversible-hydrogel-from-a-histidine-containing-tripeptide
#14
A M Garcia, M Kurbasic, S Kralj, M Melchionna, S Marchesan
We report the first histidine-containing self-assembling tripeptide devoid of capping groups that forms a thermoreversible hydrogel under physiological conditions and catalyses hydrolysis of an ester, providing a minimalist building block for functional soft materials.
June 20, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28630316/recombinant-silicateins-as-model-biocatalysts-in-organosiloxane-chemistry
#15
S Yasin Tabatabaei Dakhili, Stephanie A Caslin, Abayomi S Faponle, Peter Quayle, Sam P de Visser, Lu Shin Wong
The family of silicatein enzymes from marine sponges (phylum Porifera) is unique in nature for catalyzing the formation of inorganic silica structures, which the organisms incorporate into their skeleton. However, the synthesis of organosiloxanes catalyzed by these enzymes has thus far remained largely unexplored. To investigate the reactivity of these enzymes in relation to this important class of compounds, their catalysis of Si-O bond hydrolysis and condensation was investigated with a range of model organosilanols and silyl ethers...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28630043/the-small-gtpases-k-ras-n-ras-and-h-ras-have-distinct-biochemical-properties-determined-by-allosteric-effects
#16
Christian W Johnson, Derion Reid, Jillian A Parker, Shores Salter, Ryan Knihtila, Petr Kuzmic, Carla Mattos
H-, K-, and N-Ras are small GTPases that are important in the control of cell proliferation, differentiation and survival, and their mutants occur frequently in human cancers. The G-domain, which catalyzes GTP hydrolysis and mediates downstream signaling, is 95% conserved between the Ras isoforms. Due to their very high sequence identity, biochemical studies done on H-Ras have been considered representative of all three Ras proteins. We show here that this is not a valid assumption. Using enzyme kinetic assays under identical conditions, we observed clear differences between the three isoforms in intrinsic catalysis of GTP by Ras in the absence and presence of the Ras-binding domain (RBD) of the C-Raf Kinase protein (Raf-RBD)...
June 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28630006/multiple-conformational-states-of-dnaa-protein-regulate-its-interaction-with-dnaa-boxes-in-the-initiation-of-dna-replication
#17
Meera J Patel, Lavesh Bhatia, Gulden Yilmaz, Esther E Biswas-Fiss, Subhasis B Biswas
DnaA protein is the initiator of genomic DNA replication in prokaryotes. It binds to specific DNA sequences in the origin of DNA replication and unwinds small AT-rich sequences downstream for the assembly of the replisome. The mechanism of activation of DnaA that enables it to bind and organize the origin DNA and leads to replication initiation remains unclear. In this study, we have developed double-labeled fluorescent DnaA probes to analyze conformational states of DnaA protein upon binding DNA, nucleotide, and Soj sporulation protein using Fluorescence Resonance Energy Transfer (FRET)...
June 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28629967/isolation-of-fungi-from-dung-of-wild-herbivores-for-application-in-bioethanol-production
#18
Rhulani Makhuvele, Ignatious Ncube, Elbert Lukas Jansen van Rensburg, Daniël Coenrad La Grange
Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629709/energy-recovery-from-one-and-two-stage-anaerobic-digestion-of-food-waste
#19
Giorgia De Gioannis, Aldo Muntoni, Alessandra Polettini, Raffaella Pomi, Daniela Spiga
One- and two-stage anaerobic digestion of food waste aimed at recovering methane (CH4) and hydrogen and methane (H2+CH4), respectively, were compared in order to assess the potential benefits from the two-stage process in terms of overall energy recovery. Results suggest that a two-stage process where the first reactor is properly operated in order to achieve a significant net hydrogen production, may display a 20% comparatively higher energy recovery yield as a result, mainly, of enhanced methane production as well as of the associated hydrogen production...
June 16, 2017: Waste Management
https://www.readbyqxmd.com/read/28629408/biochemical-characterization-of-an-enantioselective-esterase-from-brevundimonas-sp-ly-2
#20
Jing Zhang, Mengjun Zhao, Die Yu, Jingang Yin, Hao Zhang, Xing Huang
BACKGROUND: Lactofen, a member of the diphenylether herbicides, has high activity and is commonly used to control broadleaf weeds. As a post-emergent herbicide, it is directly released to the environment, and easily caused the pollution. This herbicide is degraded in soil mainly by microbial activity, but the functional enzyme involved in the biodegradation of lactofen is still not clear now. RESULTS: A novel esterase gene lacH, involved in the degradation of lactofen, was cloned from the strain Brevundimonas sp...
June 19, 2017: Microbial Cell Factories
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