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https://www.readbyqxmd.com/read/29793208/enhanced-phosphate-removal-from-wastewater-by-using-in-situ-generated-fresh-trivalent-fe-composition-through-the-interaction-of-fe-ii-on-caco-3
#1
Yujie Li, Xiaoman He, Huimin Hu, Tingting Zhang, Jun Qu, Qiwu Zhang
Excessive existences of nutrients such as phosphate in the aqueous environment remain as a heavy concern although many researches have been reported for dealing with their removal. Based on the understanding toward the interactions of Fe compounds with phosphate and carbonate from many available researches, we designed a very simple and efficient approach for phosphate removal by using in situ generated fresh trivalent Fe composition through the interaction of Fe(II) as FeSO4 on CaCO3 . Addition and agitation of Fe(II) and CaCO3 simultaneously to phosphate solution allowed an amorphous Fe(III)-P or Ca-Fe(III)-P precipitation, with a phosphate removal rate close to 100%, to reduce the residual phosphorus concentration less than 0...
May 21, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29793146/development-of-a-novel-tridentate-ligand-for-colorimetric-detection-of-mn-2-based-on-agnps
#2
Jianyu Wei, Jinfan Chen, Guozong Yue, Liangsheng Hu, Danqing Zhao, Jing Zhu, Luming Yang, Deshun Huang, Pengxiang Zhao
A novel tridentate ligand nitrilotris(methylene)tris(1,2,3-triazole)triacetate (NTTTA) has been synthesized by click reaction and followed with ester hydrolysis reaction. The silver nanoparticles (AgNPs) were then modified and stabilized by this ligand, and subsequently been employed for the highly selective and sensitive colorimetric detection of Mn2+ in aqueous solution. The presence of Mn2+ can cause the aggregation of AgNPs, which leads to the color change of the dispersion from yellow to brown, as well as the decrease and red-shift of the surface plasmon resonance absorption...
May 9, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29793136/effects-of-gestational-hypertension-and-pre-eclampsia-in-mrna-expression-of-fibrinolysis-genes-in-primary-cultured-human-umbilical-vein-endothelial-cells
#3
Irais Poblete-Naredo, Yury Rodríguez-Yáñez, Rogelio O Corona-Núñez, Stuart González-Monroy, Juan E Salinas, Arnulfo Albores
Hypertension disorders (HD) and pre-eclampsia (PRE) are leading causes of maternal deaths worldwide. PRE is associated with vascular endothelial dysfunction and with deregulation of the fibrinolysis pathway genes. Fibrinolysis is the fibrin clot hydrolysis process catalyzed by plasmin, a proteolytic enzyme formed from plasminogen. Plasminogen is cleaved by tissue-type (tPA) and urokinase-type (uPA) activators and inhibited by the plasminogen activator inhibitors type-1 (PAI-1) and type-2 (PAI-2). The whole process maintains blood hemostasis...
May 17, 2018: Thrombosis Research
https://www.readbyqxmd.com/read/29793023/kinetics-and-stereochemistry-of-linb-catalyzed-%C3%AE-hbcd-transformation-comparison-of-in-vitro-and-in-silico-results
#4
Norbert V Heeb, Manuel Mazenauer, Simon Wyss, Birgit Geueke, Hans-Peter E Kohler, Peter Lienemann
LinB is a haloalkane dehalogenase found in Sphingobium indicum B90A, an aerobic bacterium isolated from contaminated soils of hexachlorocyclohexane (HCH) dumpsites. We showed that this enzyme also converts hexabromocyclododecanes (HBCDs). Here we give new insights in the kinetics and stereochemistry of the enzymatic transformation of δ-HBCD, which resulted in the formation of two pentabromocyclododecanols (PBCDols) as first- (P1δ , P2δ ) and two tetrabromocyclododecadiols (TBCDdiols) as second-generation products (T1δ , T2δ )...
May 12, 2018: Chemosphere
https://www.readbyqxmd.com/read/29792968/applicability-of-endochitinase-of-flavobacterium-johnsoniae-with-transglycosylation-activity-in-generating-long-chain-chitooligosaccharides
#5
Papa Rao Vaikuntapu, Mohan Krishna Mallakuntla, Subha Narayan Das, Bhoopal Bhuvanachandra, Bellamkonda Ramakrishna, Sandhya Rani Nadendla, Appa Rao Podile
Chitin and its derivatives are used for a variety of applications. Flavobacterium johnsoniae UW1 is an aerobic Gram-negative bacterium and genome analysis revealed the presence of 10 glycoside hydrolases (GHs) that may degrade or modify chitin. The gene encoding chitinase B (FjchiB), which encodes a single catalytic GH18 domain has been cloned and heterologously expressed in Escherichia coli. FjChiB was optimally active in 50 mM sodium citrate buffer (pH 6.0) at 40 °C. FjChiB was salt-tolerant and catalytically versatile, with substrate specificity towards 75% DDA (degree of de-acetylation) chitosan, followed by colloidal chitin...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792964/a-family-30-glucurono-xylanase-from-bacillus-subtilis-lc9-expression-characterization-and-its-application-in-chinese-bread-making
#6
Yalan Guo, Zhen Gao, Jiaxing Xu, Siyuan Chang, Bin Wu, Bingfang He
A GH30-8 endoxylanase was identified from an environmental Bacillus subtilis isolate following growth selection on aspen wood glucuronoxylan. The putative endoxylanase was cloned for protein expression and characterization in the Gram-positive protease deficient protein expression host B. subtilis WB800. The extracellular activity obtained was 55 U/mL, which was 14.5-fold higher than that obtained with the native species. The apparent molecular mass of BsXyn30 was estimated as 43 kDa by SDS-PAGE. BsXyn30 showed an optimal activity at pH 7...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792921/characterization-of-solution-stress-degradation-products-of-aliskiren-and-prediction-of-their-physicochemical-and-admet-properties
#7
Bhoopendra Singh Kushwah, Jugal Gupta, Dilip Kumar Singh, Moolchand Kurmi, Archana Sahu, Saranjit Singh
Forced degradation studies on aliskiren were carried out according to ICH and WHO guidelines. Six degradation products were formed in total in the solution state. Their separation among themselves and from the drug was successfully achieved on a C-18 column utilizing acetonitrile and phosphate buffer (pH 3.0) in the mobile phase, which was run in a gradient mode. To characterize them, a complete mass fragmentation pathway of the drug was first established with the help of MS/TOF and MSn data. This was followed by LC-MS/TOF studies on the degradation products...
May 21, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29792818/substrate-induced-formation-of-ribosomal-decoding-center-for-accurate-and-rapid-genetic-code-translation
#8
Michael Y Pavlov, Måns Ehrenberg
Accurate translation of genetic information is crucial for synthesis of functional proteins in all organisms. We use recent experimental data to discuss how induced fit affects accuracy of initial codon selection on the ribosome by aminoacyl transfer RNA in ternary complex ( T3 ) with elongation factor Tu (EF-Tu) and guanosine-5'-triphosphate (GTP). We define actual accuracy ([Formula: see text]) of a particular protein synthesis system as its current accuracy and the effective selectivity ([Formula: see text]) as [Formula: see text] in the limit of zero ribosomal binding affinity for T3 ...
May 20, 2018: Annual Review of Biophysics
https://www.readbyqxmd.com/read/29792428/structures-and-activities-of-tiahuramides-a-c-cyclic-depsipeptides-from-a-tahitian-collection-of-the-marine-cyanobacterium-lyngbya-majuscula
#9
Annabel Levert, Rebeca Alvariño, Louis Bornancin, Eliane Abou Mansour, Adam M Burja, Anne-Marie Genevière, Isabelle Bonnard, Eva Alonso, Luis Botana, Bernard Banaigs
The structures of three new cyclic depsipeptides, tiahuramides A (1), B (2), and C (3), from a French Polynesian collection of the marine cyanobacterium Lyngbya majuscula are described. The planar structures of these compounds were established by a combination of mass spectrometry and 1D and 2D NMR experiments. Absolute configurations of natural and nonproteinogenic amino acids were determined through a combination of acid hydrolysis, derivitization with Marfey's reagent, and HPLC. The absolute configuration of hydroxy acids was confirmed by Mosher's method...
May 24, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29791843/cell-cycle-regulation-of-dynamic-chromosome-association-of-the-condensin-complex
#10
Rahul Thadani, Julia Kamenz, Sebastian Heeger, Sofía Muñoz, Frank Uhlmann
Eukaryotic cells inherit their genomes in the form of chromosomes, which are formed from the compaction of interphase chromatin by the condensin complex. Condensin is a member of the structural maintenance of chromosomes (SMC) family of ATPases, large ring-shaped protein assemblies that entrap DNA to establish chromosomal interactions. Here, we use the budding yeast Saccharomyces cerevisiae to dissect the role of the condensin ATPase and its relationship with cell-cycle-regulated chromosome binding dynamics...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29791080/one-step-lignocellulose-fractionation-using-2-5-furandicarboxylic-acid-as-biogenic-and-recyclable-catalyst
#11
Dennis Weidener, Holger Klose, Walter Leitner, Ulrich Schurr, Björn Usadel, Pablo Domínguez de María, Philipp Michael Grande
To develop novel biorefinery concepts, the use of bio-based catalysts and solvents must be aligned with the Green Chemistry principles. In this context biogenic 2,5-furandicarboxylic acid (FDCA) is a very promising, yet underused molecule with high potential application as acid catalyst combining feasibility and sustainability with an efficient and straightforward recovery. In this work, FDCA was evaluated as catalyst in the recently developed OrganoCat pretreatment, a biphasic lignocellulose fractionation system...
May 23, 2018: ChemSusChem
https://www.readbyqxmd.com/read/29790507/a-novel-liquid-chromatography-detector-based-on-a-dielectric-barrier-discharge-molecular-emission-spectrometer-with-online-microwave-assisted-hydrolysis-for-determination-of-dithiocarbamates
#12
Bingjun Han, Ya Li, Bing Qian, Yan He, Lixu Peng, Huamei Yu
A novel detector for liquid chromatography (LC) for the determination of dithiocarbamate (DTC) fungicides is presented with a miniaturized dielectric barrier discharge-microplasma molecular emission spectrometer and an online microwave-assisted hydrolysis reactor. DTCs in the sample/standard solutions together with SnCl2 were pumped to the reactor to be converted to CS2 with high efficiency, which was then separated from the liquid phase and transformed for the detection of the specific molecular emission at 257...
May 23, 2018: Analyst
https://www.readbyqxmd.com/read/29789978/diauxic-growth-of-clostridium-acetobutylicum-atcc-824-when-grown-on-mixtures-of-glucose-and-cellobiose
#13
Felipe Buendia-Kandia, Emmanuel Rondags, Xavier Framboisier, Guillain Mauviel, Anthony Dufour, Emmanuel Guedon
Clostridium acetobutylicum, a promising organism for biomass transformation, has the capacity to utilize a wide variety of carbon sources. During pre-treatments of (ligno) cellulose through thermic and/or enzymatic processes, complex mixtures of oligo saccharides with beta 1,4-glycosidic bonds can be produced. In this paper, the capability of C. acetobutylicum to ferment glucose and cellobiose, alone and in mixtures was studied. Kinetic studies indicated that a diauxic growth occurs when both glucose and cellobiose are present in the medium...
May 22, 2018: AMB Express
https://www.readbyqxmd.com/read/29788884/progress-of-oral-insulin-and-related-drug-delivery-systems-and-their-pharmacokinetics
#14
Jingjing Chen, Rui Liu, Changxiao Liu, Xin Jin, Qinghua Zhang, Jialu Wang, Fang Zhao, Ze Wang, Haiyan Qiu, Yazhuo Li, Xiulin Yi
BACKGROUND: As society has developed and living standards have improved, diabetes has become a severe public health issue. Insulin plays a crucial role in managing hyperglycemia caused by type I diabetes and particular type II diabetes. Many researchers are seeking alternative, more acceptable methods of insulin delivery, such as oral insulin. An oral formulation has become a new goal for insulin delivery in recent years. METHODS: The PubMed and CNKI databases were searched for "oral insulin, " "drug delivery systems, " and "pharmacokinetics, " and 85 relevant articles were selected from the results as material for this review...
May 22, 2018: Current Drug Metabolism
https://www.readbyqxmd.com/read/29788478/a-minimal-threshold-of-fancj-helicase-activity-is-required-for-its-response-to-replication-stress-or-double-strand-break-repair
#15
Sanjay Kumar Bharti, Joshua A Sommers, Sanket Awate, Marina A Bellani, Irfan Khan, Lynda Bradley, Graeme A King, Yeonee Seol, Venkatasubramanian Vidhyasagar, Yuliang Wu, Takuye Abe, Koji Kobayashi, Kazuo Shin-Ya, Hiroyuki Kitao, Marc S Wold, Dana Branzei, Keir C Neuman, Robert M Brosh
Fanconi Anemia (FA) is characterized by bone marrow failure, congenital abnormalities, and cancer. Of over 20 FA-linked genes, FANCJ uniquely encodes a DNA helicase and mutations are also associated with breast and ovarian cancer. fancj-/- cells are sensitive to DNA interstrand cross-linking (ICL) and replication fork stalling drugs. We delineated the molecular defects of two FA patient-derived FANCJ helicase domain mutations. FANCJ-R707C was compromised in dimerization and helicase processivity, whereas DNA unwinding by FANCJ-H396D was barely detectable...
May 21, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29787882/effect-of-the-surface-acid-sites-of-tungsten-trioxide-for-highly-selective-hydrogenation-of-cellulose-to-ethylene-glycol
#16
Naixu Li, Zhongxiang Ji, Lingfei Wei, Yu Zheng, Quanhao Shen, Quanhong Ma, Menglu Tan, Mengmeng Zhan, Jiancheng Zhou
This work studied a facile and template-free hydrothermal route for controlled synthesis of tungsten trioxide in the form of hexagonal nanorod (h-WO3 ) and monoclinic nanosheet (m-WO3 ). The surface morphology, crystal plane, surface bound water, and surface acid sites of the two kinds of WO3 nanocrystals were investigated systematically. They were further evaluated as catalysts for selective cellulose hydrolysis. While both of them exhibited good catalytic performance, h-WO3 was found to be more preferential for ethylene glycol (EG) generation...
May 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29787240/sulfonium-ion-condensation-the-burden-borne-by-sam-synthetase
#17
Charles A Lewis, Richard Wolfenden
S-Adenosylmethionine (SAM+) serves as the prin-cipal methylating agent in biological systems, but the thermodynamic basis of its reactivity does not appear to have been established. Here, we show that methionine, methanol and H+ combine to form S-methylmethionine (SMM+) with a temperature-independent equilibrium constant of 9.9 M-2. The corresponding group transfer potential of SMM+, i. e. its free energy of hydrolysis at pH 7, is -8.2 kcal/mol. The "energy-rich" nature of sulfonium ions is related to the extreme acidity (pKa -5...
May 22, 2018: Biochemistry
https://www.readbyqxmd.com/read/29787238/anti-fibrotic-effect-of-ketoprofen-grafted-alginate-microcapsules-in-the-transplantation-of-insulin-producing-cells
#18
François Noverraz, Elisa Montanari, Joël Pimenta, Luca Szabo, Daniel Ortiz, Carmen Gonelle-Gispert, Léo Bühler, Sandrine Gerber-Lemaire
The controlled release of small molecular modulators of the immune response from hydrogel microspheres (MS) used for cell immobilization is an attractive approach to reduce pericapsular fibrotic overgrowth (PFO) after transplantation. Ketoprofen is a well-known non-steroidal anti-inflammatory drug involved in the early stage inflammation cascade. PEGylated derivatives of ketoprofen, presenting either ester or amide linkage to the drug, were synthesized and conjugated to the hydroxyl groups of sodium alginate (Na-alg)...
May 22, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29786939/an-update-on-enzymatic-cocktails-for-lignocellulose-breakdown
#19
REVIEW
Andreza de Mello Lopes, Edivaldo Ximenes Ferreira Filho, Leonora Rios de Souza Moreira
Alternative energy sources have received increasing attention in recent years. The possibility of adding value to agricultural wastes, by producing biofuels and other products with economic value from lignocellulosic biomass by enzymatic hydrolysis, has been widely explored. Lignocellulosic biomass, as well as being an abundant residue, is a complex recalcitrant structure that requires a consortium of enzymes for its complete degradation. Pools of enzymes with different specificities acting together usually produce an increase in hydrolysis yield...
May 22, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29785912/para-%C3%AE%C2%BA-casein-during-the-ripening-and-storage-of-low-ph-high-moisture-feta-cheese
#20
Voula Alexandraki, Golfo Moatsou
The hypothesis of this research paper was that the physicochemical conditions in a low-pH, high-moisture white brined cheese such as Feta would make para-κ-casein vulnerable to residual chymosin activity during ripening and storage. It was important to address this hypothesis, since cheese para-κ-casein could theoretically be used for the assessment of the origin of cheese milk by means of various analytical methods. Feta cheese was manufactured from sheep milk and from four different mixtures of sheep and goat milk in triplicate...
May 2018: Journal of Dairy Research
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