keyword
MENU ▼
Read by QxMD icon Read
search

hydrolysis

keyword
https://www.readbyqxmd.com/read/28527381/enantioselectivity-of-haloalkane-dehalogenase-linb-on-the-degradation-of-1-2-dichloropropane-a-qm-mm-study
#1
Xiaowen Tang, Ruiming Zhang, Yanwei Li, Qingzhu Zhang, Wenxing Wang
The hydrolysis dechlorination mechanism of a chiral organochlorinepollutant, 1,2-dichloropropane (DCP), catalyzed by haloalkane dehalogenase LinB has been investigated by using a combined quantum mechanics/molecular mechanics method. LinB was confirmed to be enantioselective towards the catabolism of the racemic mixture. Based on the SN2 nucleophilic substitution mechanism, the dechlorination process was identified as the rate-determining step in LinB-catalyzed degradation of 1,2-dichloropropane, the Boltzmann-weighted average potential barrier of which is 18...
May 12, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28527142/temporal-and-spatial-features-of-selected-wastewater-marking-pharmaceuticals-and-potential-mechanisms-of-their-removal-from-urban-rivers
#2
Haidong Zhou, Yadan Wangjin, Jianbo Liu, Tianqi Ying, Yumei Xuan
The investigations on seasonal and spatial distribution of 12 selected wastewater-marking pharmaceuticals (WWMPs) belonging to different therapeutic classes were conducted in three major urban rivers of Yangpu District, Shanghai, East China. The potential mechanisms for the removal of WWMPs in the rivers were also experimentally investigated. The detection frequencies of most WWMPs were in the range of 56-100%, with the exception of clofibric acid, which was not detected during the storm events. The median concentrations ranged from not detected to 5821 ng/L (caffeine) and the maximum concentration was 8662 ng/L, found in caffeine...
May 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28527113/mutant-p53-promotes-cell-spreading-and-migration-via-arhgap44
#3
Jinjin Xu, Jian Jiao, Wei Xu, Lei Ji, Dongjie Jiang, Shaofang Xie, Syeda Kubra, Xiaotao Li, Junjiang Fu, Jianru Xiao, Bianhong Zhang
The tumor suppressor p53 protein is either lost or mutated in about half of all human cancers. Loss of p53 function is well known to influence cell spreading, migration and invasion. While expression of mutant p53 is not equivalent to p53 loss, mutant p53 can acquire new functions to drive cell spreading and migration via different mechanisms. In our study, we found that mutant p53 significantly increased cell spreading and migration when comparing with p53-null cells. RNA-Seq analysis suggested that Rho GTPase activating protein 44 (ARHGAP44) is a new target of mutant p53, which suppressed ARHGAP44 transcription...
May 16, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28526975/lipid-nanoparticles-for-administration-of-poorly-water-soluble-neuroactive-drugs
#4
Elisabetta Esposito, Markus Drechsler, Paolo Mariani, Federica Carducci, Michela Servadio, Francesca Melancia, Patrizia Ratano, Patrizia Campolongo, Viviana Trezza, Rita Cortesi, Claudio Nastruzzi
This study describes the potential of solid lipid nanoparticles and nanostructured lipid carriers as nano-formulations to administer to the central nervous system poorly water soluble drugs. Different neuroactive drugs, i.e. dimethylfumarate, retinyl palmitate, progesterone and the endocannabinoid hydrolysis inhibitor URB597 have been studied. Lipid nanoparticles constituted of tristearin or tristearin in association with gliceryl monoolein were produced. The nanoencapsulation strategy allowed to obtain biocompatible and non-toxic vehicles, able to increase the solubility of the considered neuroactive drugs...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28526528/gluten-degrading-bacteria-are-present-in-the-human-small-intestine-of-healthy-volunteers-and-celiac-patients
#5
Alexandra R Herrán, Jénifer Pérez-Andrés, Alberto Caminero, Esther Nistal, Santiago Vivas, José María Ruiz de Morales, Javier Casqueiro
Gluten is the only known environmental factor that triggers celiac disease. Several studies have described an imbalance between the intestinal microbiota of different individuals based on diagnoses. Moreover, recent studies have suggested that human bacteria may play an important role in gluten hydrolysis. However, there has been no research focusing on the small intestine. This study aimed to characterize the adult small intestine microbiota possibly implicated in gluten hydrolysis. Duodenal biopsies from different diagnosed individuals were cultured in a gluten-containing medium, and the grown microbiota was analyzed by culture dependent/independent methods...
May 16, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28526189/potential-impact-of-salinity-on-methane-production-from-food-waste-anaerobic-digestion
#6
Jianwei Zhao, Yiwen Liu, Dongbo Wang, Fei Chen, Xiaoming Li, Guangming Zeng, Qi Yang
Previous studies have demonstrated that the presence of sodium chloride (NaCl) inhibited the production of methane from food waste anaerobic digestion. However, the details of how NaCl affects methane production from food waste remain unknown by now and the efficient approach to mitigate the impact of NaCl on methane production was seldom reported. In this paper, the details of how NaCl affects methane production was first investigated via a series of batch experiments. Experimental results showed the effect of NaCl on methane production was dosage dependent...
May 16, 2017: Waste Management
https://www.readbyqxmd.com/read/28524092/marine-fish-proteins-and-peptides-for-cosmeceuticals-a-review
#7
REVIEW
Jayachandran Venkatesan, Sukumaran Anil, Se-Kwon Kim, Min Suk Shim
Marine fish provide a rich source of bioactive compounds such as proteins and peptides. The bioactive proteins and peptides derived from marine fish have gained enormous interest in nutraceutical, pharmaceutical, and cosmeceutical industries due to their broad spectrum of bioactivities, including antioxidant, antimicrobial, and anti-aging activities. Recently, the development of cosmeceuticals using marine fish-derived proteins and peptides obtained from chemical or enzymatical hydrolysis of fish processing by-products has increased rapidly owing to their activities in antioxidation and tissue regeneration...
May 18, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28523377/mining-lipolytic-enzymes-in-community-dna-from-high-andean-soils-using-a-targeted-approach
#8
Daniel Borda-Molina, José Salvador Montaña, María Mercedes Zambrano, Sandra Baena
Microbial enrichments cultures are a useful strategy to speed up the search for enzymes that can be employed in industrial processes. Lipases have gained special attention because they show unique properties such as: broad substrate specificity, enantio- and regio-selectivity and stability in organic solvents. A major goal is to identify novel lipolytic enzymes from microorganisms living in cold extreme environments such as high Andean soils, of relevance to our study being their capability be used in industrial processes...
May 18, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28523272/structural-elements-regulating-aaa-protein-quality-control-machines
#9
REVIEW
Chiung-Wen Chang, Sukyeong Lee, Francis T F Tsai
Members of the ATPases Associated with various cellular Activities (AAA+) superfamily participate in essential and diverse cellular pathways in all kingdoms of life by harnessing the energy of ATP binding and hydrolysis to drive their biological functions. Although most AAA+ proteins share a ring-shaped architecture, AAA+ proteins have evolved distinct structural elements that are fine-tuned to their specific functions. A central question in the field is how ATP binding and hydrolysis are coupled to substrate translocation through the central channel of ring-forming AAA+ proteins...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/28522698/oligogalacturonic-acid-inhibits-vascular-calcification-by-two-mechanisms-inhibition-of-vascular-smooth-muscle-cell-osteogenic-conversion-and-interaction-with-collagen
#10
Ahmed Hodroge, Eric Trécherel, Marjorie Cornu, Walaa Darwiche, Ali Mansour, Katia Ait-Mohand, Thomas Verissimo, Cathy Gomila, Carole Schembri, Sophie Da Nascimento, Redouan Elboutachfaiti, Agnès Boullier, Emmanuel Lorne, Josiane Courtois, Emmanuel Petit, Sylvestre Toumieux, José Kovensky, Pascal Sonnet, Ziad A Massy, Saïd Kamel, Claire Rossi, Jérôme Ausseil
OBJECTIVE: Cardiovascular diseases constitute the leading cause of mortality worldwide. Calcification of the vessel wall is associated with cardiovascular morbidity and mortality in patients having many diseases, including diabetes mellitus, atherosclerosis, and chronic kidney disease. Vascular calcification is actively regulated by inductive and inhibitory mechanisms (including vascular smooth muscle cell adaptation) and results from an active osteogenic process. During the calcification process, extracellular vesicles (also known as matrix vesicles) released by vascular smooth muscle cells interact with type I collagen and then act as nucleating foci for calcium crystallization...
May 18, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28522606/structural-analysis-of-the-dual-function-thioesterase-sav606-unravels-the-mechanism-of-michael-addition-of-glycine-to-an-%C3%AE-%C3%AE-unsaturated-thioester
#11
Taichi Chisuga, Akimasa Miyanaga, Fumitaka Kudo, Tadashi Eguchi
Thioesterases catalyze hydrolysis of acyl thioesters to release carboxylic acid or macrocyclization to produce the corresponding macrocycle in the biosynthesis of fatty acids, polyketides, or nonribosomal peptides. Recently, we reported that the thioesterase CmiS1 from Streptomyces sp. MJ635-86F5 catalyzes the Michael addition of glycine to an α,β-unsaturated fatty acyl thioester followed by thioester hydrolysis in the biosynthesis of the macrolactam antibiotic cremimycin. However, the molecular mechanisms of CmiS1-catalyzed reactions are unclear...
May 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28522605/structure-function-analysis-of-human-sucrase-isomaltase-identifies-key-residues-required-for-catalytic-activity
#12
Birthe Gericke, Natalie Schecker, Mahdi Amiri, Hassan Y Naim
Sucrase-isomaltase (SI) is an intestinal membrane-associated α-glucosidase that breaks down di- and oligosaccharides to absorbable monosaccharides. SI has two homologous functional subunits (sucrase and isomaltase) that both belong to the glycoside hydrolase family 31 (GH31) and differ in substrate specificity. All GH31 enzymes share a consensus sequence harboring an aspartic acid residue as a catalytic nucleophile. Moreover, crystallographic structural analysis of isomaltase predicts that another aspartic acid residue functions as a proton donor in hydrolysis...
May 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28522398/study-on-the-synthesis-and-physicochemical-properties-of-starch-acetate-with-low-substitution-under-microwave-assistance
#13
Derong Lin, Wei Zhou, Jingjing Zhao, Weijie Lan, Rongming Chen, Yutong Li, Baoshan Xing, Zhuohao Li, Mengshi Xiao, Zhijun Wu, Xindan Li, Rongna Chen, Xingwen Zhang, Hong Chen, Qing Zhang, Wen Qin, Suqing Li
In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied. A three-level-three-factorial Central Composite Design using Response Surface Methodology was employed to optimize the reaction conditions. The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W. These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied...
May 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28522005/biocompatible-microcrystalline-cellulose-particles-from-cotton-wool-and-magnetization-via-a-simple-in-situ-co-precipitation-method
#14
Mehnaz Rashid, Mohammad Abdul Gafur, Mostafa Kaiyum Sharafat, Hideto Minami, Mohd Abdul Jalil Miah, Hasan Ahmad
This investigation describes the preparation of magnetically doped degradable microcrystalline cellulose (MCC) nanocomposite particles with application potential in biotechnology, solid support for biomolecule/water purification, oil recovery from water and beyond. MCC was first extracted from cotton wool, the most abundant biocompatible polymer, by sulfuric acid hydrolysis and the effect of acid strength was examined. The size of the elongated fiber structure was reduced with increasing acid strength. MCC particles extracted by treatment with 70% sulfuric acid were used to prepare magnetic MCC nanocomposite particles...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521991/nmr-characterization-of-cellulose-acetate-mole-fraction-of-monomers-in-cellulose-acetate-determined-from-carbonyl-carbon-resonances
#15
Hiroyuki Kono, Chinatsu Oka, Ryota Kishimoto, Sayaka Fujita
Cellulose acetate (CA) samples with varying degrees of substitution were prepared via homogeneous acetylation in 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) and by the acid-hydrolysis of cellulose triacetate in acetic acid. Quantitative analysis of the (13)C NMR spectra facilitated the assignment of the carbonyl carbon shifts of the 2-mono-, 3-mono-, 6-mono-, 2,3-di-, 2,6-di-, 3,6-di-, and 2,3,6-tri-substituted anhydroglucose units (AGUs), and the determination of the mole fraction of 7 AGUs and unsubstituted AGU in the CA chains...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521816/biochemical-characterization-of-the-xylan-hydrolysis-profile-of-the-extracellular-endo-xylanase-from-geobacillus-thermodenitrificans-t12
#16
Martinus J A Daas, Patricia Murciano Martínez, Antonius H P van de Weijer, John van der Oost, Willem M de Vos, Mirjam A Kabel, Richard van Kranenburg
BACKGROUND: Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is present in most strains belonging to this genus. As part of the HUS locus, the xynA gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. Several Geobacillus endo-xylanases have been characterized for their optimum temperature, optimum pH and generation of degradation products...
May 18, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28521372/synthesis-and-characterization-of-new-azecine-derivatives-as-potential-neuroleptics
#17
Stephanie Zergiebel, Christian Fleck, Hans-Dieter Arndt, Christoph Enzensperger, Andreas Seeling
Dibenzo- and benzindolo-azecines represent a class of potential neuroleptics. To characterize the effectiveness at the dopamine and 5-HT2A-receptor representative structures were synthesized and tested by radio ligand binding studies, in vivo and in vitro studies.Neuroleptic potency and the risk of side effects of the prodrug 7-methyl-5,6,7,8,9,14-hexahydrodibenzo[d,g]azecin-3-yl isobutyrate, an ester derivative of the most promising azecine 7-methyl-5,6,7,8,9,14-hexahydrodibenzo[d,g]azecin-3-ol (LE404), was tested in vivo concerning conditioned avoidance response inhibition, locomotor activity and triggering of catalepsy vs...
May 18, 2017: Drug Research
https://www.readbyqxmd.com/read/28521324/the-role-of-hydrolyzed-formula-in-allergy-prevention
#18
Michael D Cabana
Asthma, eczema, food allergy, and allergic rhinitis are some of the most common pediatric, chronic conditions in the world. Breastfeeding is the optimal way to feed all infants. For those infants who are exposed to infant formula, some studies suggest that certain partially hydrolyzed or extensively hydrolyzed formulas may decrease the risk of allergic disease compared to nonhydrolyzed formulas for children with a family history of atopic disease. Overall, there is some evidence to suggest that partially hydrolyzed whey formulas and extensively hydrolyzed casein formulas may decrease the risk of developing eczema for infants at high risk of allergic disease...
2017: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/28521226/phosphate-recovery-from-hydrothermally-treated-sewage-sludge-using-struvite-precipitation
#19
M T Munir, Bing Li, Irina Boiarkina, Saeid Baroutian, Wei Yu, Brent R Young
New technologies are needed to recover phosphate from organic wastes, such as sewage sludge. Sewage sludge can be hydrothermally treated to make it safe but this process is expensive. Recovering a valuable by-product, such as phosphate, could improve the economics of hydrothermal treatment. Therefore, the technical and preliminary differential cost analysis of combining hydrothermal treatment with phosphate recovery (by precipitation of magnesium ammonium phosphate (struvite)) was investigated. The effects of pH, magnesium ion dose, and either wet oxidation or thermal hydrolysis hydrothermal treatment were examined...
May 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521209/use-of-solid-digestate-for-lignocellulolytic-enzymes-production-through-submerged-fungal-fermentation
#20
Alida Musatti, Elena Ficara, Chiara Mapelli, Cecilia Sambusiti, Manuela Rollini
Studies were performed on the use of the solid fraction of digestate (D) for the production of lignocellulolytic enzymes (endo- and exo-glucanase, xylanase, β-glucosidase and laccase) by fungi, in comparison with wheat straw (benchmark) (W). To date, this is the first report on the use of such an inexpensive substrate in a liquid environment. Submerged instead of solid state fermentation was applied to overcome pH inhibition and increase surface accessibility. A total of 21 fungal strains were tested: the most performing ones were Irpex lacteus DSM1183 for both β-glucosidase (52 IU/g with D, + 400% compared to W) and endo-glucanase (236 IU/g with D, + 470% compared to W), Schizophyllum commune CBS30132 for xylanase (715 IU/g with W, + 145% compared to D) and Pleurotus ostreatus ATCC96997 for laccase (124 IU/g with D, +230% compared to D)...
May 15, 2017: Journal of Environmental Management
keyword
keyword
94381
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"