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https://www.readbyqxmd.com/read/29328971/ultrasensitive-electrochemical-sensing-of-hg2-based-on-thymine-hg2-thymine-interaction-and-signal-amplification-of-alkaline-phosphatase-catalyzed-silver-deposition
#1
Aigui Xu, Long Chao, Hongbo Xiao, Yuyun Sui, Jia Liu, Qingji Xie, Shouzhuo Yao
We report an ultrasensitive electrochemical sensor for Hg2+ detection, on the basis of two Hg2+-specific oligonucleotide probes (a thiolated capture probe and a biotinated signal probe), "terminal" signal amplification of alkaline phosphatase catalyzed deposition of silver and in situ microliter-droplet anodic stripping voltammetry (ASV). The thiolated capture probe is immobilized on an Au-plated glassy carbon electrode, and the biotinated signal probe is then attached to the electrode surface through the thymine-Hg2+-thymine interaction in the presence of Hg2+...
January 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29324918/specific-neck-pain-algometric-measurements-and-their-relation-to-heart-rate-and-skin-humidity
#2
Evgenia Petala, Maria Mironidou-Tzouveleki, Kimon Nanassis, Eirini Pagkalidou, Christos Kyriakou, Dorothea Kapoukranidou
Cervical pain is very common in general population but only few methods have been used to evaluate it with objectiveness. There are only few studies which use algometer as a means of neck pain assessment. Studies have shown that algometer could be used in pain evaluation but more studies are necessary to support this. The main purpose of the study was the evaluation of algometer as a means of an objective measurement of pain threshold to people with non-specific neck pain. The study focused on the search of correlation between neck pain and pain pressure threshold (PPT) after recording the number of the minimum pain feeling in the sample with the pressure of the algometer at specific neck points...
September 2017: Hellenic Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/29324310/25-hydroxyl-protopanaxatriol-protects-against-h2o2-induced-h9c2-cardiomyocytes-injury-via-pi3k-akt-pathway-and-apoptotic-protein-down-regulation
#3
Zhihao Wang, Guangyue Su, Zhiguo Zhang, Han Dong, Yuehui Wang, Huiying Zhao, Yuqing Zhao, Qi Sun
Oxidative stress injury and apoptosis are the main mechanisms of myocardial ischemia-reperfusion injury (MI/RI). Compounds with anti-oxidant properties can treat MI/RI. Therefore, identification of natural antioxidants such as herbs or botanical drugs is an ideal strategy to develop safe and effective anti-MI/RI drugs. Cardioprotective effects of Ginseng are well documented and are attributable to its anti-oxidant, anti-inflammatory, anti-tumorigenic, anti-arrhythmic, anti-ischemic properties. Ginseng monomer 20®-dammarane -3 beta,6 alpha,12 beta,20,25-pentol(25-hydroxyl-Protopanaxatriol,25-OH-PPT), a novel compound, which belongs to panaxatriol category, is extracted from the leaves and stem of ginseng...
January 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29322943/ultra-sensitive-and-selective-detection-of-mercury-ion-hg2-using-free-standing-silicon-nanowire-sensors
#4
Yan Jin, Anran Gao, Qing-Hui Jin, Tie Li, Yuelin Wang, Jian-Long Zhao
In this paper, ultra-sensitive and highly selective Hg2+ detection in aqueous solutions was studied by free-standing silicon nanowire (SiNW) sensors. The all-around surface of SiNW arrays was functionalized with (3-Mercaptopropyl)trimethoxysilane (MPTMS) serving as Hg2+ sensitive layer. Due to effective electrostatic control provided by the free-standing structure, the detection limit as low as 1 ppt was obtained. A linear relationship (R2=0.9838) between log(CHg2+) and device current change from 1 ppt to 5 ppm was observed...
January 11, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29319536/new-photoplethysmogram-indicators-for-improving-cuffless-and-continuous-blood-pressure-estimation-accuracy
#5
Wan-Hua Lin, Hui Wang, Oluwarotimi Williams Samuel, Gengxing Liu, Zhen Huang, Guanglin Li
Objective: The accuracy of cuffless and continuous blood pressure (BP) estimation has been improved, but it is still unsatisfactory for clinical uses. This study was designed to further increase BP estimation accuracy. Approach: In this study, a number of new indicators were extracted from photoplethysmogram (PPG) recordings and linear regression method was used to construct BP estimation models based on the PPG indicators and pulse transit time (PTT). The performance of the BP estimation models was evaluated by the PPG recordings from 22 subjects when they performed mental arithmetic stress and Valsalva's manoeuvre tasks that could induce BP fluctuations...
December 28, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/29318417/identifying-a-major-qtl-associated-with-salinity-tolerance-in-nile-tilapia-using-qtl-seq
#6
Xiao Hui Gu, Dan Li Jiang, Yan Huang, Bi Jun Li, Chao Hao Chen, Hao Ran Lin, Jun Hong Xia
Selection of new lines with high salinity tolerance allows for economically feasible production of tilapias in brackish water areas. Mapping QTLs and identifying the markers linked to salinity-tolerant traits are the first steps in the improvement of the tolerance in tilapia through marker-assisted selection techniques. By using QTL-seq strategy and linkage-based analysis, two significant QTL intervals (chrLG4 and chrLG18) on salinity-tolerant traits were firstly identified in the Nile tilapia. Fine mapping with microsatellite and SNP markers suggested a major QTL region that located at 23...
January 9, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29316386/changes-in-pain-sensitization-after-bariatric-surgery
#7
Joshua J Stefanik, David T Felson, Caroline M Apovian, Jingbo Niu, M Margaret Clancy, Michael P LaValley, Tuhina Neogi
OBJECTIVE: To evaluate changes in pain (at the knee and elsewhere) and pain sensitization in obese persons with knee pain undergoing bariatric surgery compared with similarly obese persons undergoing medical management. METHODS: Individuals undergoing bariatric surgery and medical management were recruited. Knee pain severity of the more painful knee (index knee) was assessed at baseline and 12 months using the WOMAC. Pressure pain threshold (PPT) was assessed at the index patella and the right wrist...
January 5, 2018: Arthritis Care & Research
https://www.readbyqxmd.com/read/29306188/ginsenosides-and-ginsenosidases-in-the-pathobiology-of-ginseng-cylindrocarpon-destructans-zinss-scholten
#8
Jiao Wang, Honglei Chen, Juan Gao, Jixun Guo, Xuesong Zhao, Yifa Zhou
To investigate the role that ginsenosides (and some of their metabolites) play in interactions between plants and phytopathogenic fungi (e.g. Cylindrocarpon destructans (Zinss) Scholten), we systematically determined the anti-fungal activities of six major ginsenosides (Rb1, Rb2, Rc, Rd, Re and Rg1), along with the metabolites of ginsenoside Rb1 (Gypenoside XVII (G-XVII) and F2), against the ginseng root pathogen C. destructans (Zinss) Scholten and non-ginseng pathogens Fusarium graminearum Schw., Exserohilum turcicum (Pass...
December 29, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29304407/the-effect-of-chlorpyrifos-on-salinity-acclimation-of-juvenile-rainbow-trout-oncorhynchus-mykiss
#9
Bagher Mojazi Amiri, Elvis Genbo Xu, Allison Kupsco, Marissa Giroux, Mahbubeh Hoseinzadeh, Daniel Schlenk
As a part of their unique life cycle, most salmonids undergo a transition from fresh water to salt water requiring various adjustments in metabolism, osmoregulation and ion regulation. Exposure to pesticides may affect the acclimation of juvenile salmonids to salt water during downstream migration to estuaries. Using the Caspian Sea as a model waterbody, the present study aimed to determine how the toxicity of the organophosphate pesticide chlorpyrifos (CPF) impacts saline acclimation of rainbow trout (Oncorhynchus mykiss)...
December 28, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/29288855/biomechanical-analysis-of-porous-additive-manufactured-cages-for-lateral-lumbar-interbody-fusion-a-finite-element-analysis
#10
Zhenjun Zhang, Hui Li, Guy R Fogel, Zhenhua Liao, Yang Li, Weiqiang Liu
BACKGROUND: A porous additive manufactured (AM) cage may provide stability similar to that of traditional solid cages, and may be beneficial to bone ingrowth. The biomechanical influence of various porous cages on stability, subsidence, stresses in cage, and facet contact force has not been fully described. The purpose of this study was to verify biomechanical effects of porous AM cages. METHODS: The surgical FE models with various cages were constructed. The partially porous titanium (PPT) cages and fully porous titanium (FPT) cages were applied...
December 27, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29287180/neurocognitive-and-physical-functioning-in-the-seveso-women-s-health-study
#11
Jennifer Ames, Marcella Warner, Paolo Brambilla, Paolo Mocarelli, William A Satariano, Brenda Eskenazi
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is neurotoxic in animals but few studies have investigated its effects on the human brain. Related dioxin-like compounds have been linked to poorer cognitive and motor function in older adults, with effects more pronounced in women, perhaps due to the loss of neuro-protective estrogen in menopause. On 10 July 1976, a chemical explosion in Seveso, Italy, resulted in one of the highest known residential exposures to TCDD. In 1996, we initiated the Seveso Women's Health Study, a retrospective cohort study of the health of the women who were newborn to 40 years old in 1976...
December 26, 2017: Environmental Research
https://www.readbyqxmd.com/read/29285672/stimulatory-effect-of-dietary-taurine-on-growth-performance-digestive-enzymes-activity-antioxidant-capacity-and-tolerance-of-common-carp-cyprinus-carpio-l-fry-to-salinity-stress
#12
Mohsen Abdel-Tawwab, Mohamed N Monier
The present study was carried out to evaluate the effect of dietary taurine (Tau) on performance, digestive enzymes, antioxidant activity, and resistance of common carp, Cyprinus carpio L., fry to salinity stress. Fish (0.97 ± 0.033 g) were fed on different taurine levels of 0.0 (control), 5, 10, 15, or 20 g/kg diet up to satiation twice daily for 8 weeks. At the end of the feeding trial, fish were stressed by exposure to 10 ppt salinity for 3 days during which fish mortality was observed. Fish performance was significantly (P < 0...
December 28, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29281118/estrogen-deficiency-mediated-m2-macrophage-osteoclastogenesis-contributes-to-m1-m2-ratio-alteration-in-ovariectomized-osteoporotic-mice
#13
Ce Dou, Ning Ding, Chunrong Zhao, Tianyong Hou, Fei Kang, Zhen Cao, Chuan Liu, Yun Bai, Qijie Dai, Qinyu Ma, Fei Luo, Jianzhong Xu, Shiwu Dong
In this study, for the first time we discovered that M1/M2 ratio is increased in bone marrow of ovariectomized (OVX) osteoporotic C57BL/6 mice. Considering estrogen is the main variable, we assumed that estrogen participated in this alteration. To determine whether and how estrogen contributes to the change of M1/M2 ratio, we first isolated bone marrow macrophages (BMMs) from mice femur and stimulated the cells with lipopolysaccharide (LPS)/Interferon (IFN)-γ for M1 polarization and interleukin (IL)-4/IL-13 for M2 polarization...
December 27, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29277262/pancreatectomies-for-pancreatic-neoplasms-in-pediatric-and-adolescent-age-a-single-institution-experience
#14
Chiara Scandavini, Roberto Valente, Elena Rangelova, Ralf Segersvärd, Urban Arnelo, Johan Permert, Pär-Johan Svensson, Jakob Stenman, Marco Del Chiaro
BACKGROUND: There are very few data in the current literature regarding the short- and long-term outcome of surgery for pediatric pancreatic tumors (PPT). No data are available on the impact of pancreatic surgery on the children's growth. METHODS: This is a retrospective cohort study on a consecutive series of pediatric/adolescent patients who underwent pediatric surgery at Karolinska University Hospital from January 2005 to July 2017. RESULTS: Overall 14 pancreatic operations were performed in 13 patients...
December 19, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/29275794/being-adaptive-to-pain-enhances-sham-acupuncture-analgesia-a-crossover-healthy-human-study
#15
Zhen Zheng, Dawn Wong Lit Wan, Lars Arendt-Nielsen, Dong Y Yao, Genevieve Iversen, Charlie C Xue, Kelun Wang
We have reported a model that distinguishes pain adaptive individuals (PA) from those who are pain non-adaptive (PNA). The present randomised, cross-over, participant-assessor blinded study aimed to determine the impact of pain adaptability on individuals' response to real and sham acupuncture. Healthy volunteers (nine PA and 13 PNA) were randomly allocated to receive real and sham acupuncture on the left hand and forearm in two separate acupuncture sessions. Pressure pain thresholds (PPTs) were measured at bilateral forearms and right leg before, immediately after and 20 minutes after the end of acupuncture...
December 2017: Journal of Acupuncture and Meridian Studies
https://www.readbyqxmd.com/read/29275446/distinct-expression-of-phenotypic-markers-in-placodes-and-neural-crest-derived-afferent-neurons-innervating-the-rat-stomach
#16
Alzbeta Trancikova, Eva Kovacova, Fei Ru, Kristian Varga, Mariana Brozmanova, Milos Tatar, Marian Kollarik
BACKGROUND: Visceral pain is initiated by activation of primary afferent neurons among which the capsaicin-sensitive (TRPV1-positive) neurons play an important role. The stomach is a common source of visceral pain. Similar to other organs, the stomach receives dual spinal and vagal afferent innervation. Developmentally, spinal dorsal root ganglia (DRG) and vagal jugular neurons originate from embryonic neural crest and vagal nodose neurons originate from placodes. In thoracic organs the neural crest- and placodes-derived TRPV1-positive neurons have distinct phenotypes differing in activation profile, neurotrophic regulation and reflex responses...
December 23, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29275437/gene-expression-of-thyrotropin-and-corticotrophin-releasing-hormones-is-regulated-by-environmental-salinity-in-the-euryhaline-teleost-sparus-aurata
#17
Ignacio Ruiz-Jarabo, J A Martos-Sitcha, C Barragán-Méndez, G Martínez-Rodríguez, J M Mancera, F J Arjona
In euryhaline teleosts, the hypothalamus-pituitary-thyroid and hypothalamus-pituitary-interrenal axes (HPT and HPI, respectively) are regulated in response to environmental stimuli such as salinity changes. However, the molecular players participating in this physiological process in the gilthead seabream (Sparus aurata), a species of high value for aquaculture, are still not identified and/or fully characterized in terms of gene expression regulation. In this sense, this study identifies and isolates the thyrotropin-releasing hormone (trh) mRNA sequence from S...
December 23, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29274449/proton-mrs-detection-of-high-lipid-levels-and-low-adc-is-characteristics-of-germinoma
#18
Fumiyuki Yamasaki, Yasuyuki Kinoshita, Takeshi Takayasu, Satoshi Usui, Manish Kolakshyapati, Motoki Takano, Sayuru Tsuyuguchi, Vishwa Jeet Amatya, Yuji Akiyama, Yukio Takeshima, Kazuhiko Sugiyama, Kaoru Kurisu
BACKGROUND: The differentiation of germinoma from other tumors by conventional MRI can be very difficult. The purpose of our study was to determine if proton magnetic resonance spectroscopy and diffusion-weighted imaging could give additional information for definitive diagnosis of germinoma. MATERIALS AND METHODS: Our institutional review board approved this retrospective study. We reviewed the images of 26 patients with histologically confirmed germinoma who were treated in our institution between 2003 and 2016...
December 20, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29273967/molecular-characteristics-of-a-novel-hsp60-gene-and-its-differential-expression-in-manila-clams-ruditapes-philippinarum-under-thermal-and-hypotonic-stress
#19
Jianfeng Ding, Jia Li, Dongmin Yang, Feng Yang, Hongtao Nie, Zhongming Huo, Xiwu Yan
The Manila clam Ruditapes philippinarum inhabits the intertidal zone and must therefore tolerate broad fluctuations in water temperature and salinity. Heat shock protein 60 (HSP60) is an evolutionarily conserved, multi-functional protein that plays a significant role in protecting organisms from harmful stress conditions. We cloned the R. philippinarum HSP60 (RpHSP60) gene and analyzed its transcriptional responses to thermal and low-salinity stresses. The complete sequence of RpHSP60 cDNA was 1777 nucleotides, containing a 1728-bp open reading frame encoding a polypeptide of 576-amino acids, with a calculated molecular mass of 61...
December 22, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29260444/utility-of-a-patient-reported-outcome-in-measuring-functional-impairment-during-autologous-stem-cell-transplant-in-patients-with-multiple-myeloma
#20
Nina Shah, Qiuling Shi, Sergio Giralt, Loretta Williams, Qaiser Bashir, Muzaffar Qazilbash, Richard E Champlin, Charles S Cleeland, Xin Shelley Wang
BACKGROUND: We aimed to determine the utility of a patient-reported outcome (PRO) as it relates to patient performed testing (PPT) for measuring functional status in multiple myeloma patients after autologous hematopoietic stem cell transplantation (auto-HCT). METHODS: Symptom interference on walking (a PRO) was measured by the MD Anderson Symptom Inventory (MDASI). PPT was assessed via 6-min walk test (6MWT). Mixed effects modeling was used to examine (1) the longitudinal relationship between the MDASI score and 6MWT distance and (2) the MDASI scores between patients who did or did not complete the 6WMT...
December 19, 2017: Quality of Life Research
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