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https://www.readbyqxmd.com/read/29147970/effect-of-different-salinities-on-gene-expression-and-activity-of-digestive-enzymes-in-the-thick-lipped-grey-mullet-chelon-labrosus
#1
REVIEW
I M Pujante, F J Moyano, J A Martos-Sitcha, J M Mancera, G Martínez-Rodríguez
The effects of different environmental salinities (0, 12, 40, and 55 ppt) on pepsinogen 2 (pga2), trypsinogen 2 (try2), chymotrypsinogen (ctr), and pancreatic alpha-amylase (amy2a) gene expression, and on the total activities of their corresponding enzymes, were assessed in Chelon labrosus juveniles, after their corresponding full-complementary DNA sequences were cloned. Furthermore, the quantitative effect of different salinities on the hydrolysis of feed protein by fish digestive enzymes was evaluated using an in vitro system...
November 17, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29137402/bdnf-trkb-signaling-mediates-the-anorectic-action-of-estradiol-in-the-nucleus-tractus-solitarius
#2
Ling Shen, David Q H Wang, Meifeng Xu, Stephen C Woods, Min Liu
Although compelling evidence indicates that estradiol (E2) acts in the nucleus tractus solitarius (NTS) to reduce food intake, the underlying mechanisms are largely unknown. We now report that estrogen's anorectic action occurs through enhancing the strength of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase (TrkB) signaling in the NTS. Intra-4th-ventricular administration of a low dose of BDNF reduced food intake to a greater extent in ovariectomized (OVX) rats cyclically treated with E2 than in vehicle-treated OVX rats, implying that cyclic E2 replacement increases BDNF's satiating potency...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29135458/immediate-effects-of-osteopathic-treatment-versus-therapeutic-exercise-on-patients-with-chronic-cervical-pain
#3
Xabier Galindez-Ibarbengoetxea, Igor Setuain, Robinson Ramírez-Velez, Lars L Andersen, Miriam González-Izal, Andoni Jauregi, Mikel Izquierdo
Context • Alternatives to medication for immediate pain relief are needed because long-term use of pain medication has been associated with adverse effects. High-velocity, low-amplitude (HVLA) manipulation techniques (MTs) and craniocervical flexion (CCF) exercises have been frequently used in the management of chronic cervical pain. Objective • The study intended to compare the immediate effects on pain of a treatment using HVLA manipulation versus one using a CCF exercise protocol. Design • The research team designed a single-blinded, randomized clinical trial...
November 14, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/29128393/rem-sleep-deprivation-and-dopaminergic-d2-receptors-modulation-increase-recognition-memory-in-an-animal-model-of-parkinson-s-disease
#4
Adriano D S Targa, Ana Carolina D Noseda, Lais S Rodrigues, Mariana F Aurich, Marcelo M S Lima
Cognitive impairment is an important non-motor symptom of Parkinson's disease (PD). The neuronal death in nigrostriatal pathway is the main factor for motor symptoms and recent studies indicate a possible influence in non-motor symptoms as well. The pedunculopontine tegmental nucleus (PPT) and basal ganglia are closely related anatomically and functionally and, since they are affected by neurodegeneration in PD, they might be involved in recognition memory. To investigate this, we promoted an ibotenic acid lesion within the PPT or a rotenone lesion within substantia nigra pars compacta (SNpc) of Wistar rats, followed by 24h of REM sleep deprivation (REMSD)...
November 8, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/29128248/adsorption-of-1-2-3-trichloropropane-tcp-to-meet-a-mcl-of-5%C3%A2-ppt
#5
Roger W Babcock, Bryce K Harada, Krishna M Lamichhane, Korey T Tsubota
1,2,3-Trichloropropane (TCP) is a groundwater contaminant in the drinking water aquifers in Hawaii and some other states. Granular activated carbon (GAC) has been used for 30 years to treat approximately 60 million gallons per day of TCP-contaminated groundwater in Hawaii. The State of Hawaii's current maximum contaminant level (MCL) for TCP is 600 ng/L (ppt), and consideration is being given to lower the MCL to 5 ppt. There is no EPA MCL for TCP. A study was conducted to determine if any GAC could meet a 5 ppt MCL for TCP, and if so, how many bedvolumes (BVs) could be treated prior to breakthrough...
November 8, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29124884/utility-of-cremophor-rh-40-as-a-micellar-improvement-for-spectrofluorimetric-estimation-of-sorafenib-in-pure-form-commercial-preparation-and-human-plasma
#6
Dalia W Zidan, Manal S Elmasry, Wafaa S Hassan, Abdalla A Shalaby
An easy, quick, simple and accurate spectrofluorimetric method was recognized and validated for evaluation of sorafenib (SOR) in pure form and biologically in plasma. Cremophor RH 40 (Cr RH 40) used for enhancing the fluorescence activity of SOR in phosphate buffer (pH 7). Cr RH 40 improved the native fluorescence of SOR remarkably in water. The fluorescence spectrum of SOR was observed at 405 nm after excitation at 265 nm. The linearity appeared to be in the range of 5 to 600 ng ml(-1) for pure and from 9 to 500 ng ml(-1) for plasma using the protein precipitation (ppt) method while from 10 to 500 ng ml(-1) for plasma using liquid-liquid extraction method...
November 10, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/29122886/mechanism-of-polypurine-tract-primer-generation-by-hiv-1-reverse-transcriptase
#7
Małgorzata Figiel, Miroslav Krepl, Sangwoo Park, Jarosław Poznański, Krzysztof Skowronek, Agnieszka Gołąb, Taekjip Ha, Jiři Šponer, Marcin Nowotny
HIV-1 reverse transcriptase (HIV-1 RT) possesses both DNA polymerase activity and RNase H activity that act in concert to convert single-stranded RNA of the viral genome to double-stranded DNA that is then integrated into the DNA of the infected cell. Reverse transcriptase-catalyzed reverse transcription critically relies on the proper generation of a polypurine tract (PPT) primer. However, the mechanism of PPT primer generation and the features of the PPT sequence that are critical for its recognition by HIV-1 RT remain unclear...
November 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29121466/reaching-a-vapor-sensitivity-of-0-01-parts-per-quadrillion-in-the-screening-of-large-volume-freight
#8
Daoiz Zamora, Mario Amo-Gonzalez, Marta Lanza, Gonzalo Fernandez De La Mora, Juan Fernandez de la Mora
The feasibility of detecting explosives in the atmosphere at concentrations as low as 0.01 ppq hinges on the poorly known question of what interfering species exist at these or higher concentrations. To clarify the issue, hundreds of samples of ambient air, either clean or loaded with explosives (from lightly contaminated environments) have been collected in fiberglass/stainless steel filters coated with Tenax-GR, thermally desorbed at variable temperature, and ionized with Cl- via secondary electrospray (SESI)...
November 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29121095/the-pedunculopontine-tegmentum-controls-renal-sympathetic-nerve-activity-and-cardiorespiratory-activities-in-nembutal-anesthetized-rats
#9
Anne M Fink, Caron Dean, Mariann R Piano, David W Carley
Elevated renal sympathetic nerve activity (RSNA) accompanies a variety of complex disorders, including obstructive sleep apnea, heart failure, and chronic kidney disease. Understanding pathophysiologic renal mechanisms is important for determining why hypertension is both a common sequelae and a predisposing factor of these disorders. The role of the brainstem in regulating RSNA remains incompletely understood. The pedunculopontine tegmentum (PPT) is known for regulating behaviors including alertness, locomotion, and rapid eye movement sleep...
2017: PloS One
https://www.readbyqxmd.com/read/29116831/clinician-friendly-physical-performance-tests-for-the-knee
#10
Jerrod J Harrison, Marissa K Yorgey, Alexander J Csiernik, Joseph H Vogler, Kenneth E Games
Reference:  Hegedus EJ, McDonough S, Bleakley C, Cook CE, Baxter GD. Clinician-friendly lower extremity physical performance measures in athletes: a systematic review of measurement properties and correlation with injury. Part 1: the tests for knee function including the hop tests. Br J Sports Med. 2015;49(10):642-648. CLINICAL QUESTION:   Do individual physical performance tests (PPTs) used as measures for lower extremity function have any relationship to injuries in athletes aged 12 years or older? DATA SOURCES:   Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to locate articles...
November 8, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/29112383/ozone-formation-induced-by-the-impact-of-reactive-bromine-and-iodine-species-on-photochemistry-in-a-polluted-marine-environment
#11
Moshe Shechner, Eran Tas
Reactive iodine and bromine species (RIS and RBS, respectively) are known for altering atmospheric chemistry, causing sharp tropospheric ozone (O3) depletion in polar regions and significant O3 reduction in the marine boundary layer (MBL). Here we use measurement-based modelling to show that, unexpectedly, both RIS and RBS can lead to enhanced O3 formation in a polluted marine environment under volatile organic compound (VOC)-limited conditions associated with high nitrogen oxide (NOX = [NO] + [NO2]) concentrations...
November 7, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29110195/a-transposition-active-phyllostachys-edulis-long-terminal-repeat-ltr-retrotransposon
#12
Mingbing Zhou, Linlin Liang, Heikki Hänninen
Due to infrequent sexual reproduction, moso bamboo breeding by hybridization is extremely technically difficult. Insertional mutagenesis based on endogenous active transposons may thus serve as an alternative method to create new germplasm of moso bamboo. In the present study, using LTR-STRUC, a full-length intact long terminal repeat (LTR) retrotransposon was identified in the moso bamboo genome and was named PHRE2 (Phyllostachys edulis retrotransposon 2). The 5' and 3' LTR sequences of PHRE2 were highly (98...
November 6, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/29110066/ecological-patterns-among-bacteria-and-microbial-eukaryotes-derived-from-network-analyses-in-a-low-salinity-lake
#13
Adriane Clark Jones, K David Hambright, David A Caron
Microbial communities are comprised of complex assemblages of highly interactive taxa. We employed network analyses to identify and describe microbial interactions and co-occurrence patterns between microbial eukaryotes and bacteria at two locations within a low salinity (0.5-3.5 ppt) lake over an annual cycle. We previously documented that the microbial diversity and community composition within Lake Texoma, southwest USA, were significantly affected by both seasonal forces and a site-specific bloom of the harmful alga, Prymnesium parvum...
November 7, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/29100805/development-of-the-modified-simple-test-for-evaluating-hand-function-modified-stef-construct-reliability-validity-and-responsiveness
#14
Keisuke Irie, Hirokatsu Iseki, Satomi Okamoto, Kazuhiro Okamoto, Seiji Nishimura, Kenji Kagechika
STUDY DESIGN: Clinimetric evaluation study. INTRODUCTION: Despite the availability of numerous performance tests to measure finger dexterity, there is no international consensus on hand function evaluation. PURPOSE OF THE STUDY: To evaluate the reliability, validity, and responsiveness of the modified version of the simple test for evaluating hand function (STEF), which is widely used in Japan. METHODS: The intrarater (n = 40) and inter-rater (n = 32) reliability of the modified STEF was evaluated by calculating the intraclass correlation coefficient (ICC), models (1,1) and (2,1), respectively, in healthy individuals...
November 1, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
https://www.readbyqxmd.com/read/29100761/effective-phospholipid-removal-from-plasma-samples-by-solid-phase-extraction-with-the-use-of-copper-ii-modified-silica-gel-cartridges
#15
Jolanta Flieger, Małgorzata Tatarczak-Michalewska, Anna Kowalska, Anna Madejska, Tomasz Śniegocki, Anna Sroka-Bartnicka, Monika Szymańska-Chargot
The new sorbent for solid phase extraction (SPE), based on silica gel modified with a copper (II), was obtained and its application for phospholipids removal from the human plasma was tested. SPE column conditioning requirements, the volume of the plasma, the composition of the elution solvent were all established. The efficacy of the removal of phospholipids was compared for different methods such as standard protein precipitation or HybridSPE Phospholipid Ultra and HybridSPE-PPT. The sample clean-up was verified by mass spectrometry (MS) and by monitoring of chromatograms in the region between 190nm and 400nm...
October 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29097982/evaluating-hand-function-in-clients-with-trigger-finger
#16
Danit Langer, Adina Maeir, Michael Michailevich, Shai Luria
Background: Trigger finger (TF) is a common hand pathology frequently encountered in hand clinics. Occupational therapists predominantly assess TF symptoms as opposed to using standardized hand functioning assessments. The purpose of this study was to assess the construct validity of dexterity and grip strength assessments for clients with TF. Method: Sixty-three participants with TF and 66 healthy controls were administered the Functional Dexterity Test (FDT), Purdue Pegboard Test (PPT), and Jamar® Hydraulic Hand Dynamometer (JD) and completed the Disabilities of Arm Shoulder and Hand questionnaire (DASH)...
2017: Occupational Therapy International
https://www.readbyqxmd.com/read/29090429/evolvement-of-transgenic-male-sterility-and-fertility-restoration-system-in-rice-for-production-of-hybrid-varieties
#17
Gundra Sivakrishna Rao, Priyanka Deveshwar, Malini Sharma, Sanjay Kapoor, Khareedu Venkateswara Rao
We have developed a unique male-sterility and fertility-restoration system in rice by combining Brassica napus cysteine-protease gene (BnCysP1) with anther-specific P12 promoter of rice for facilitating production of hybrid varieties. In diverse crop plants, male-sterility has been exploited as a useful approach for production of hybrid varieties to harness the benefits of hybrid vigour. The promoter region of Os12bglu38 gene of rice has been isolated from the developing panicles and was designated as P12. The promoter was fused with gusA reporter gene and was expressed in Arabidopsis and rice systems...
October 31, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29081683/physical-activity-may-be-associated-with-conditioned-pain-modulation-in-women-but-not-men-among-healthy-individuals
#18
Yukiko Shiro, Tatsunori Ikemoto, Yuta Terasawa, Young-Chang P Arai, Kazuhiro Hayashi, Takahiro Ushida, Takako Matsubara
BACKGROUND: Conditioned pain modulation (CPM), a phenomenon also known as diffuse noxious inhibitory control, is thought to be affected by various factors, including sex and level of physical activity. However, the involvement of these factors in CPM remains unclear. METHODS: Eighty-six healthy young subjects (M/F, 43/43) participated in this study. Participants were assessed on the basis of their mechanical pressure pain threshold (PPT), CPM response, body mass index (BMI), basal metabolic rate (BMR), and duration of moderate-to-vigorous physical activity (MVPA) over a week, using a motion counter...
2017: Pain Research & Management: the Journal of the Canadian Pain Society
https://www.readbyqxmd.com/read/29076519/arterial-stiffness-alone-does-not-explain-arteriovenous-fistula-outcomes
#19
Damian G McGrogan, Stephanie Stringer, Paul Cockwell, Mark Jesky, Charlie Ferro, Alexander P Maxwell, Nicholas G Inston
INTRODUCTION: Marked arterial adaptation is critical in permitting and sustaining the increased blood flow within an arteriovenous fistula (AVF). The aim of this investigation was to evaluate markers of arterial disease and their association with the early post-operative AVF outcomes. METHODS: We included all patients in whom an AVF had been performed after enrolment to the Renal Impairment In Secondary Care (RIISC) study. Primary AVF failure (PFL) was defined as thrombosis at six-week review...
October 26, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/29075862/concomitant-external-pneumatic-compression-treatment-with-consecutive-days-of-high-intensity-interval-training-reduces-markers-of-proteolysis
#20
Cody T Haun, Michael D Roberts, Matthew A Romero, Shelby C Osburn, James C Healy, Angelique N Moore, Christopher B Mobley, Paul A Roberson, Wesley C Kephart, Petey W Mumford, Michael D Goodlett, David D Pascoe, Jeffrey S Martin
PURPOSE: To compare the effects of external pneumatic compression (EPC) and sham when used concurrently with high intensity interval training (HIIT) on performance-related outcomes and recovery-related molecular measures. METHODS: Eighteen recreationally endurance-trained male participants (age: 21.6 ± 2.4 years, BMI: 25.7 ± 0.5 kg/m(2), VO2peak: 51.3 ± 0.9 mL/kg/min) were randomized to balanced sham and EPC treatment groups. Three consecutive days of HIIT followed by EPC/sham treatment (Days 2-4) and 3 consecutive days of recovery (Days 5-7) with EPC/sham only on Days 5-6 were employed...
October 26, 2017: European Journal of Applied Physiology
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