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https://www.readbyqxmd.com/read/29048467/identification-of-copy-number-variations-and-translocations-in-cancer-cells-from-hi-c-data
#1
Abhijit Chakraborty, Ferhat Ay
Motivation: Eukaryotic chromosomes adapt a complex and highly dynamic three-dimensional (3D) structure, which profoundly affects different cellular functions and outcomes including changes in epigenetic landscape and in gene expression. Making the scenario even more complex, cancer cells harbor chromosomal abnormalities (e.g., copy number variations (CNVs) and translocations) altering their genomes both at the sequence level and at the level of 3D organization. High-throughput chromosome conformation capture techniques (e...
October 18, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29045270/macroglossia-during-awake-craniotomy-a-near-miss
#2
John Cormack, Shravya Karna
No abstract text is available yet for this article.
October 17, 2017: Journal of Neurosurgical Anesthesiology
https://www.readbyqxmd.com/read/29043045/radseq-dataset-with-90-missing-data-fully-resolves-recent-radiation-of-petalidium-acanthaceae-in-the-ultra-arid-deserts-of-namibia
#3
Erin A Tripp, Yi-Hsin Erica Tsai, Yongbin Zhuang, Kyle G Dexter
Deserts, even those at tropical latitudes, often have strikingly low levels of plant diversity, particularly within genera. One remarkable exception to this pattern is the genus Petalidium (Acanthaceae), in which 37 of 40 named species occupy one of the driest environments on Earth, the Namib Desert of Namibia and neighboring Angola. To contribute to understanding this enigmatic diversity, we generated RADseq data for 47 accessions of Petalidium representing 22 species. We explored the impacts of 18 different combinations of assembly parameters in de novo assembly of the data across nine levels of missing data plus a best practice assembly using a reference Acanthaceae genome for a total of 171 sequence datasets assembled...
October 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29036316/the-role-of-small-rna-based-epigenetic-silencing-for-purifying-selection-on-transposable-elements-in-capsella-grandiflora
#4
Robert Horvath, Tanja Slotte
To avoid negative effects of transposable element (TE) proliferation, plants epigenetically silence TEs using a number of mechanisms, including RNA-directed DNA methylation. These epigenetic modifications can extend outside the boundaries of TE insertions and lead to silencing of nearby genes, resulting in a trade-off between TE silencing and interference with nearby gene regulation. Therefore, purifying selection is expected to remove silenced TE insertions near genes more efficiently and prevent their accumulation within a population...
September 27, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29030401/a-comparison-of-whole-genome-sequencing-to-multigene-panel-testing-in-hypertrophic-cardiomyopathy-patients
#5
Allison L Cirino, Neal K Lakdawala, Barbara McDonough, Lauren Conner, Dale Adler, Mark Weinfeld, Patrick O'Gara, Heidi L Rehm, Kalotina Machini, Matthew Lebo, Carrie Blout, Robert C Green, Calum A MacRae, Christine E Seidman, Carolyn Y Ho
BACKGROUND: As DNA sequencing costs decline, genetic testing options have expanded. Whole exome sequencing and whole genome sequencing (WGS) are entering clinical use, posing questions about their incremental value compared with disease-specific multigene panels that have been the cornerstone of genetic testing. METHODS AND RESULTS: Forty-one patients with hypertrophic cardiomyopathy who had undergone targeted hypertrophic cardiomyopathy genetic testing (either multigene panel or familial variant test) were recruited into the MedSeq Project, a clinical trial of WGS...
October 2017: Circulation. Cardiovascular Genetics
https://www.readbyqxmd.com/read/29028690/adverse-event-reporting-harnessing-residents-to-improve-patient-safety
#6
Sarah E Tevis, Ryan K Schmocker, Tosha B Wetterneck
OBJECTIVES: Reporting of adverse and near miss events are essential to identify system level targets to improve patient safety. Resident physicians historically report few events despite their role as front-line patient care providers. We sought to evaluate barriers to adverse event reporting in an effort to improve reporting. Our main outcomes were as follows: resident attitudes about event reporting and the frequency of event reporting before and after interventions to address reporting barriers...
October 13, 2017: Journal of Patient Safety
https://www.readbyqxmd.com/read/29028240/tobacco-still-a-major-killer-will-we-achieve-the-end-game
#7
Luis Saboga-Nunes, Diane Levin-Zamir, Vance Rabius
The game of tobacco use began in Europe in 1560 when the first tobacco seeds were sent from Lisbon to the king of France, by Jean Nicot. From kings' and nobles' exclusive use, it gradually and progressively became popular among the public, as a new player. Eighty-eight years ago (1929), Fritz Linkint, an extraordinary researcher in Germany, while reviewing existing evidence regarding a wide range of cancers potentially caused by smoking, indicated that smoking was a cause of respiratory disease. Despite the overwhelming accumulated evidence of the negative effects of nicotine intake, the prevalence of tobacco use is not expected to decline in the near future...
October 1, 2017: European Journal of Public Health
https://www.readbyqxmd.com/read/29026470/major-determinants-of-maternal-near-miss-and-mortality-at-the-maternity-teaching-hospital-erbil-city-iraq
#8
Vian Sabri Akrawi, Tariq Salman Al-Hadithi, Namir Ghanim Al-Tawil
OBJECTIVES: To find out the major determinants of maternal near-miss (NM)and maternal deaths (MDs) in Erbil city, Iraq, by comparative analysis of maternal NMs and MDs. METHODS: We conducted a hospital-based cross-sectional study in the Maternity Teaching Hospital in Erbil city from 1 June to 31 December 2013. All MDs and NMs that occurred in the hospital during the study period were included in the study. Systematic identification of all eligible women was done...
September 2017: Oman Medical Journal
https://www.readbyqxmd.com/read/29023218/out-of-hospital-pediatric-patient-safety-events-results-of-the-csi-chart-review
#9
Garth Meckler, Matthew Hansen, William Lambert, Kerth O'Brien, Caitlin Dickinson, Kathryn Dickinson, Joshua Van Otterloo, Jeanne-Marie Guise
OBJECTIVE: Studies of adult hospital patients have identified medical errors as a significant cause of morbidity and mortality. Little is known about the frequency and nature of pediatric patient safety events in the out-of-hospital setting. We sought to quantify pediatric patient safety events in EMS and identify patient, call, and care characteristics associated with potentially severe events. METHODS: As part of the Children's Safety Initiative -EMS, expert panels independently reviewed charts of pediatric critical ambulance transports in a metropolitan area over a three-year period...
October 12, 2017: Prehospital Emergency Care
https://www.readbyqxmd.com/read/28989879/root-cause-analysis-of-diabetic-ketoacidosis-admissions-at-a-tertiary-referral-pediatric-emergency-department-in-north-india
#10
Muralidharan Jayashree, Rohit Sasidharan, Sunit Singhi, Karthi Nallasamy, Mullai Baalaaji
OBJECTIVES: To identify system-based factors contributing to Emergency Department (ED) admissions of children with diabetic ketoacidosis (DKA) and related complications with emphasis on parental and physician awareness and prereferral management. MATERIALS AND METHODS: A prospective observational root cause analysis study of all consecutive admissions of children with DKA to pediatric ED of a tertiary care referral hospital in northern India over a period of 1 year (July 2010-June 2011)...
September 2017: Indian Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28982617/ointment-fistulography-introducing-a-novel-technique-for-single-or-multiple-urethrocutaneous-fistula-diagnosis-after-hypospadias-surgery
#11
Sarah Mozafarpour, Abdol-Mohammad Kajbafzadeh, Reza Abbasioun, Ali Akbar Habibi, Behnam Nabavizadeh
INTRODUCTION AND OBJECTIVE: Hypospadias is a common congenital malformation of the male genital tract. The most frequent complication after hypospadias repair is urethrocutaneous fistula.(1) Its incidence has been reported up to 35% worldwide.(2) The diagnosis of these fistulas is sometimes challenging particularly with tiny and multiple fistulas. Usually, parents complain of urinary spraying, sprinkling, or passing a single stream of urine from the undersurface of the penis after the surgery...
August 2017: Urology
https://www.readbyqxmd.com/read/28979044/use-of-volatile-anesthetic-agent-in-extracorporeal-circuit-as-a-cause-of-break-in-polycarbonate-connector-lessons-learnt
#12
Deepak Gowda, K Rashmi, Naveen Pandarinathan, Neelam Desai
Mishaps, near misses, and lethal incidents are known to occur during cardiopulmonary bypass. We share one such rare case of break in polycarbonate connector because of the use of isoflurane in extracorporeal circuit and its successful management.
September 2017: Journal of Extra-corporeal Technology
https://www.readbyqxmd.com/read/28969858/predictors-of-the-frequency-and-subjective-experience-of-cycling-near-misses-findings-from-the-first-two-years-of-the-uk-near-miss-project
#13
Rachel Aldred, Anna Goodman
Using 2014 and 2015 data from the UK Near Miss Project, this paper examines the stability of self-report incident rates for cycling near misses across these two years. It further examines the stability of the individual-level predictors of experiencing a near miss, including what influences the scariness of an incident. The paper uses three questions asked for only in 2015, which allow further exploration of factors shaping near miss rates and impacts of incidents. Firstly, a respondent's level of cycling experience; secondly, whether an incident was perceived as deliberate; and finally, whether the respondent themselves described the incident as a 'near miss' (as opposed to only a frightening and/or annoying non-injury incident)...
September 30, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28969656/inadequate-programming-insufficient-communication-and-non-compliance-with-the-basic-principles-of-maternal-death-audits-in-health-districts-in-burkina-faso-a-qualitative-study
#14
Boukaré Congo, Djénéba Sanon, Tieba Millogo, Charlemagne Marie Ouedraogo, Wambi Maurice E Yaméogo, Ziemlé Clement Meda, Seni Kouanda
BACKGROUND: Implementation of quality maternal death audits requires good programming, good communication and compliance with core principles. Studies on compliance with core principles in the conduct of maternal death audits (MDAs) exist but were conducted in urban areas, at the 2nd or 3rd level of the healthcare system, in experimental situations, or in a context of skills-building projects or technical platforms with an emphasis on the review of "near miss". This study aims to fill the gap of evidence on the implementation of MDAs in rural settings, at the first level of care and in the routine care situation in Burkina Faso...
September 29, 2017: Reproductive Health
https://www.readbyqxmd.com/read/28969226/perilunate-dislocation-case-report-and-review-of-literature
#15
Karthik Subramanian, Bakul Arora, Abhinav Bhatnagar, Imran Jan
Perilunate dislocations are rare injuries comprising of less than 10% of all wrist injuries. These usually occur after high-energy trauma to the wrist. One-fourth (25%) of perilunate dislocations are missed at the initial presentation. We report a case of perilunate dislocation in a patient who presented late, about two months after trauma. He underwent open reduction through a single dorsal approach and internal fixation with K-wires. Aggressive physiotherapy exercises were started after removal of K-wires...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28966086/actin-turnover-in-lamellipodial-fragments
#16
Dikla Raz-Ben Aroush, Noa Ofer, Enas Abu-Shah, Jun Allard, Oleg Krichevsky, Alex Mogilner, Kinneret Keren
Actin turnover is the central driving force underlying lamellipodial motility. The molecular components involved are largely known, and their properties have been studied extensively in vitro. However, a comprehensive picture of actin turnover in vivo is still missing. We focus on fragments from fish epithelial keratocytes, which are essentially stand-alone motile lamellipodia. The geometric simplicity of the fragments and the absence of additional actin structures allow us to characterize the spatiotemporal lamellipodial actin organization with unprecedented detail...
October 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28966027/microplastic-sampling-with-the-avani-trawl-compared-to-two-neuston-trawls-in-the-bay-of-bengal-and-south-pacific
#17
Marcus Eriksen, Max Liboiron, Tim Kiessling, Louis Charron, Abigail Alling, Laurent Lebreton, Heather Richards, Barent Roth, Nicolas C Ory, Valeria Hidalgo-Ruz, Erika Meerhoff, Carolynn Box, Anna Cummins, Martin Thiel
Many typical neuston trawls can only be used during relatively calm sea states and slow tow speeds. During two expeditions to the Bay of Bengal and the eastern South Pacific we investigated whether the new, high-speed AVANI trawl (All-purpose Velocity Accelerated Net Instrument) collects similar amounts and types of microplastics as two established scientific trawl designs, the manta trawl and the DiSalvo neuston net. Using a 335 μm net, the AVANI trawl can collect microplastics from the sea surface at speeds up to 8 knots as it "skis" across the surface, whereas the manta and DiSalvo neuston trawls must be towed slowly in a less turbulent sea state and often represent shorter tow lengths...
September 28, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28964048/relative-contributions-of-specific-frequency-bands-to-the-loudness-of-broadband-sounds
#18
Walt Jesteadt, Sara M Walker, Oluwaseye A Ogun, Brenda Ohlrich, Katyarina E Brunette, Marcin Wróblewski, Kendra K Schmid
Listeners with normal hearing (NH) and sensorineural hearing loss (SNHL) were asked to compare pairs of noise stimuli and choose the louder noise in each pair. Each noise was made up of 15, two-ERBN (equivalent rectangular bandwidth) wide frequency bands that varied independently over a 12-dB range from one presentation to the next. Mean levels of the bands followed the long-term average speech spectrum (LTASS) or were set to 43, 51, or 59 dB sound pressure level (SPL). The relative contribution of each band to the total loudness of the noise was determined by computing the correlation between the difference in levels for a given band on every trial and the listener's decision on that trial...
September 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28963302/building-a-collaborative-culture-in-cardiothoracic-operating-rooms-pre-and-postintervention-study-protocol-for-evaluation-of-the-implementation-of-teamstepps-training-and-the-impact-on-perceived-psychological-safety
#19
Aaron Benjamin Dahl, Arbi Ben Abdallah, Hersh Maniar, Michael Simon Avidan, Mara L Bollini, George Alexander Patterson, Aaron Steinberg, Katie Scaggs, Brenda V Dribin, Clare H Ridley
INTRODUCTION: The importance of effective communication, a key component of teamwork, is well recognised in the healthcare setting. Establishing a culture that encourages and empowers team members to speak openly in the cardiothoracic (CT) operating room (OR) is necessary to improve patient safety in this high-risk environment. METHODS AND ANALYSIS: This study will take place at Barnes-Jewish Hospital, an academic hospital in affiliation with Washington University School of Medicine located in the USA...
September 27, 2017: BMJ Open
https://www.readbyqxmd.com/read/28958987/tablet-based-patient-centered-decision-support-for-minor-head-injury-in-the-emergency-department-pilot-study
#20
Navdeep Singh, Erik Hess, George Guo, Adam Sharp, Brian Huang, Maggie Breslin, Edward Melnick
BACKGROUND: The Concussion or Brain Bleed app is a clinician- and patient-facing electronic tool to guide decisions about head computed tomography (CT) use in patients presenting to the emergency department (ED) with minor head injury. This app integrates a patient decision aid and clinical decision support (using the Canadian CT Head Rule, CCHR) at the bedside on a tablet computer to promote conversations around individualized risk and patients' specific concerns within the ED context...
September 28, 2017: JMIR MHealth and UHealth
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