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Viral mirna

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https://www.readbyqxmd.com/read/29772717/a-rapid-screen-for-host-encoded-mirnas-with-inhibitory-effects-against-ebola-virus-using-a-transcription-and-replication-competent-virus-like-particle-system
#1
Zhongyi Wang, Jiaming Li, Yingying Fu, Zongzheng Zhao, Chunmao Zhang, Nan Li, Jingjing Li, Hongliang Cheng, Xiaojun Jin, Bing Lu, Zhendong Guo, Jun Qian, Linna Liu
MicroRNAs (miRNAs) may become efficient antiviral agents against the Ebola virus (EBOV) targeting viral genomic RNAs or transcripts. We previously conducted a genome-wide search for differentially expressed miRNAs during viral replication and transcription. In this study, we established a rapid screen for miRNAs with inhibitory effects against EBOV using a tetracistronic transcription- and replication-competent virus-like particle (trVLP) system. This system uses a minigenome comprising an EBOV leader region, luciferase reporter, VP40, GP, VP24, EBOV trailer region, and three noncoding regions from the EBOV genome and can be used to model the life cycle of EBOV under biosafety level (BSL) 2 conditions...
May 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29766833/human-micrornas-expression-profiles-in-influenza-b-virus-infected-cells-based-on-illumina-miseq-platform
#2
Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Suthat Sangchuwong, Trairak Pisitkun, Yong Poovorawan, Sunchai Payungporn
BACKGROUND: Influenza B virus causes influenza-like illness in humans. MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression through mRNA degradation or translational repression. MiRNAs have evolved to regulate many cellular processes including the viral infection response. OBJECTIVE: This study aims to investigate the miRNA profiles of human cells infected with influenza B virus. METHODS: A549 cells were infected with influenza B viruses (MOI = 0...
May 14, 2018: MicroRNA
https://www.readbyqxmd.com/read/29766503/transcriptome-analysis-of-potential-mirna-involved-in-adipogenic-differentiation-of-c2c12-myoblasts
#3
Ye Hou, Liangliang Fu, Jingjin Li, Jingxuan Li, Yunxia Zhao, Yu Luan, An Liu, Huiying Liu, Xinyun Li, Shuhong Zhao, Changchun Li
>Emerging evidence indicates that microRNA (miRNA) plays an important role in adipogenesis and disease pathogenesis. To investigate the miRNA involved in regulating different periods of adipogenesis, we performed a comprehensive study on microRNAome during the stimulation of adipogenesis by an adipogenic differentiation cocktail in C2C12 myoblasts at 0, 2, 4, and 7 days using the Solexa sequencing technology. In this study, we identified 52 differentially expressed (DE) miRNA. Functional annotation indicated that the target genes of DE miRNA were mostly enriched in adipogenic transdifferentiation and fat metabolism-related pathways, including Wnt, mitogen-activated protein kinase (MAPK), and insulin signaling...
May 16, 2018: Lipids
https://www.readbyqxmd.com/read/29742983/dead-box-helicases-the-yin-and-yang-roles-in-viral-infections
#4
Vanessa Meier-Stephenson, Tyler Mrozowich, Mimi Pham, Trushar R Patel
Viruses hijack the host cell machinery and recruit host proteins to aid their replication. Several host proteins also play vital roles in inhibiting viral replication. Emerging class of host proteins central to both of these processes are the DEAD-box helicases: a highly conserved family of ATP-dependent RNA helicases, bearing a common D-E-A-D (Asp-Glu-Ala-Asp) motif. They play key roles in numerous cellular processes, including transcription, splicing, miRNA biogenesis and translation. Though their sequences are highly conserved, these helicases have quite diverse roles in the cell...
May 9, 2018: Biotechnology & Genetic Engineering Reviews
https://www.readbyqxmd.com/read/29740327/extracellular-vesicles-as-carriers-of-non-coding-rnas-in-liver-diseases
#5
REVIEW
Junfa Yang, Changyao Li, Lei Zhang, Xiao Wang
Extracellular vesicles (EVs) are small membranous vesicles secreted from normal, diseased, and transformed cells in vitro and in vivo . EVs have been found to play a critical role in cell-to-cell communication by transferring non-coding RNAs (ncRNAs) including microRNAs (miRNAs), long ncRNAs (lncRNAs) and so on. Emerging evidence shows that transferring biological information through EVs to neighboring cells in intercellular communication not only keep physiological functions, but also participate in the pathogenesis of liver diseases...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29733865/microrna-146-5p-mir-let-7c-5p-mir-221-and-mir-345-5p-are-differentially-expressed-in-respiratory-syncytial-virus-rsv-persistently-infected-hep-2-cells
#6
Eilam-Frenkel B, Naaman H, Berkic G, I Veksler-Lublinsky, G Rall, Shemer Y, Gopas J
Many viruses can establish non-cytolytic, chronic infections in host cells. Beyond the intrinsically interesting questions of how this long-term parasitism is achieved, persistently infected cells can be useful to study virus-host interactions. MicroRNAs (miRNAs) are a class of noncoding RNAs transcribed from the genomes of all multicellular organisms and some viruses. Individual miRNAs may regulate several hundred genes. In this research we have studied the expression of a selective group of host-cell encoded miRNAs, as expressed in a Respiratory Syncytial Virus (RSV) persistently infected HEp-2 cell line (HEp-2+RSV-GFP)...
May 4, 2018: Virus Research
https://www.readbyqxmd.com/read/29720520/herpes-simplex-virus-2-latency-associated-transcript-lat-region-mutations-do-not-identify-a-role-for-lat-associated-micro-rnas-in-viral-reactivation-in-the-guinea-pig-genital-model
#7
Yoshiki Kawamura, Marta Bosch-Marce, Shuang Tang, Amita Patel, Philip R Krause
Despite the long-standing observation that herpes simplex virus (HSV) Latency-Associated Transcript (LAT) promoter-deletion viruses show impaired recurrence phenotypes in relevant animal models, the mechanism by which these sequences exert this phenotypic effect is unknown. We constructed and evaluated four mutant HSV-2 viruses with targeted mutations in the LAT promoter and LAT-associated miRNAs affecting (1) the LAT TATA box, (2) the LAT ICP4-binding site, (3) miR-I and miR-II (miR-I/II), which both target ICP34...
May 2, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29717211/up-regulation-of-microrna-203-in-influenza-a-virus-infection-inhibits-viral-replication-by-targeting-dr1
#8
Sen Zhang, Jing Li, Junfeng Li, Yinhui Yang, Xiaoping Kang, Yuchang Li, Xiaoyan Wu, Qingyu Zhu, Yusen Zhou, Yi Hu
MicroRNAs (miRNAs) are small noncoding RNA molecules that play important roles in various biological processes. Much evidence shows that miRNAs are closely associated with numerous virus infections; however, involvement of cellular miRNAs in influenza A virus (IAV) infection is unclear. Here, we found that expression of miR-203 was up-regulated markedly via two different mechanisms during IAV infection. First, we examined the effects of type I interferon induced by IAV on direct activation of miR-203 expression...
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29704894/virulent-duck-enteritis-virus-infected-def-cells-generate-a-unique-pattern-of-viral-micrornas-and-a-novel-set-of-host-micrornas
#9
Xianglong Wu, Renyong Jia, Jiakun Zhou, Mingshu Wang, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Kunfeng Sun, Qiao Yang, Ying Wu, Zhongqiong Yin, Xiaoyue Chen, Jue Wang, Anchun Cheng
BACKGROUND: Duck enteritis virus (DEV) belongs to the family Herpesviridae and is an important epornitic agent that causes economic losses in the waterfowl industry. The Chinese virulent (CHv) and attenuate vaccines (VAC) are two different pathogenic DEV strains. MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate gene expression in viral infection. Nonetheless, there is little information on virulent duck enteritis virus (DEV)-encoded miRNAs. RESULTS: Using high-throughput sequencing, we identified 39 mature viral miRNAs from CHv-infected duck embryo fibroblasts cells...
April 28, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29695698/-protection-of-lymphocytes-against-hiv-using-lentivirus-vector-carrying-a-combination-of-trim5%C3%AE-hrh-genes-and-microrna-against-ccr5
#10
D O Omelchenko, D V Glazkova, E V Bogoslovskaya, F A Urusov, Y A Zhogina, G M Tsyganova, G A Shipulin
Gene therapy is considered a promising approach to treating infections caused by human immunodeficiency virus (HIV). One strategy is to introduce antiviral genes into cells in order to impart resistance to HIV. In this work, the antiviral activity of new anti-HIV lentiviral vector pT has been studied. The vector carries a combination that consists of two identical artificial miRNA mic13lg and the TRIM5α-HRH gene. Two mic13lg microRNAs suppress the expression of the CCR5 gene, which encodes the HIV coreceptor and, thus, prevents the penetration of R5-tropic HIV strains into the cell...
March 2018: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/29691416/virus-encoded-mirnas-in-ebola-virus-disease
#11
Janice Duy, Anna N Honko, Louis A Altamura, Sandra L Bixler, Suzanne Wollen-Roberts, Nadia Wauquier, Aileen O'Hearn, Eric M Mucker, Joshua C Johnson, Joshua D Shamblin, Justine Zelko, Miriam A Botto, James Bangura, Moinya Coomber, M Louise Pitt, Jean-Paul Gonzalez, Randal J Schoepp, Arthur J Goff, Timothy D Minogue
Ebola virus (EBOV) is a negative-strand RNA virus that replicates in the cytoplasm and causes an often-fatal hemorrhagic fever. EBOV, like other viruses, can reportedly encode its own microRNAs (miRNAs) to subvert host immune defenses. miRNAs are short noncoding RNAs that can regulate gene expression by hybridizing to multiple mRNAs, and viral miRNAs can enhance viral replication and infectivity by regulating host or viral genes. To date, only one EBOV miRNA has been examined in human infection. Here, we assayed mouse, rhesus macaque, cynomolgus macaque, and human samples infected with three EBOV variants for twelve computationally predicted viral miRNAs using RT-qPCR...
April 24, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29691336/a-viral-suppressor-modulates-the-plant-immune-response-early-in-infection-by-regulating-microrna-activity
#12
Robert Pertermann, Selvaraj Tamilarasan, Torsten Gursinsky, Giorgio Gambino, Jana Schuck, Claus Weinholdt, Hauke Lilie, Ivo Grosse, Ralph Peter Golbik, Vitantonio Pantaleo, Sven-Erik Behrens
Many viral suppressors (VSRs) counteract antiviral RNA silencing, a central component of the plant's immune response by sequestration of virus-derived antiviral small interfering RNAs (siRNAs). Here, we addressed how VSRs affect the activities of cellular microRNAs (miRNAs) during a viral infection by characterizing the interactions of two unrelated VSRs, the Tombusvirus p19 and the Cucumovirus 2b, with miRNA 162 (miR162), miR168, and miR403. These miRNAs regulate the expression of the important silencing factors Dicer-like protein 1 (DCL1) and Argonaute proteins 1 and 2 (AGO1 and AGO2), respectively...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29686252/micro-rnas-from-dna-viruses-are-found-widely-in-plasma-in-a-large-observational-human-population
#13
Milka Koupenova, Eric Mick, Heather A Corkrey, Tianxiao Huan, Lauren Clancy, Ravi Shah, Emelia J Benjamin, Daniel Levy, Evelyn A Kurt-Jones, Kahraman Tanriverdi, Jane E Freedman
Viral infections associate with disease risk and select families of viruses encode miRNAs that control an efficient viral cycle. The association of viral miRNA expression with disease in a large human population has not been previously explored. We sequenced plasma RNA from 40 participants of the Framingham Heart Study (FHS, Offspring Cohort, Visit 8) and identified 3 viral miRNAs from 3 different human Herpesviridae. These miRNAs were mostly related to viral latency and have not been previously detected in human plasma...
April 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29679644/non-viral-based-mir-delivery-and-recent-developments
#14
REVIEW
Annalise Elizabeth Labatut, George Mattheolabakis
miRNAs are promising therapeutic targets or tools for the treatment of numerous diseases, with most prominently, cancer. The inherent capacity of these short nucleic acids to regulate multiple cancer-related pathways simultaneously has prompted strong research on understanding miR functions and their potential use for therapeutic purposes. A key determinant of miR therapeutics' potential for treatment is their delivery. Viral and non-viral vectors attempt to address the major limitations associated with miR delivery, but several hurdles have been identified...
April 18, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29679569/ddx1-regulates-alternative-splicing-and-insulin-secretion-in-pancreatic-%C3%AE-cells
#15
Wen Zhong, Zonghong Li, Maoge Zhou, Tao Xu, You Wang
DEAD-box helicase 1 (DDX1) is a multifunction protein involved in diverse cellular processes including transcription, viral replication, mRNA/miRNA processing, and tRNA splicing. Here, we report a novel function of DDX1 in mRNA alternative splicing in pancreatic β cells. By performing integrated data analysis of high-throughput RNA sequencing (RNA-Seq), and cross-linking and immunoprecipitation coupled with deep sequencing (CLIP-Seq), we identify hundreds of alternative splicing genes that are targeted by DDX1...
June 7, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29679565/activation-of-tlr3-and-its-adaptor-ticam-1-increases-mir-21-levels-in-extracellular-vesicles-released-from-human-cells
#16
Yoshimi Fukushima, Masaaki Okamoto, Kana Ishikawa, Takahisa Kouwaki, Hirotake Tsukamoto, Hiroyuki Oshiumi
Pattern-recognition receptors (PRRs) recognizes viral RNAs and trigger the innate immune responses. Toll-like receptor 3 (TLR3), a PRR, recognizes viral double-stranded RNA (dsRNA) in endolysosomes, whereas cytoplasmic dsRNA is sensed by another PRR, MDA5. TLR3 and MDA5 utilize TICAM-1 and MAVS, respectively, to trigger the signal for inducing innate immune responses. Extracellular vesicles (EVs) include the exosomes and microvesicles; an accumulating body of evidence has shown that EVs delivers functional RNA, such as microRNAs (miRNAs), to other cells and thus mediate intercellular communications...
April 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29677213/ifn-%C3%AE-and-micrornas-are-important-modulators-of-the-pulmonary-innate-immune-response-against-influenza-a-h1n2-infection-in-pigs
#17
Louise Brogaard, Lars E Larsen, Peter M H Heegaard, Christian Anthon, Jan Gorodkin, Ralf Dürrwald, Kerstin Skovgaard
The innate immune system is paramount in the response to and clearance of influenza A virus (IAV) infection in non-immune individuals. Known factors include type I and III interferons and antiviral pathogen recognition receptors, and the cascades of antiviral and pro- and anti-inflammatory gene expression they induce. MicroRNAs (miRNAs) are increasingly recognized to participate in post-transcriptional modulation of these responses, but the temporal dynamics of how these players of the antiviral innate immune response collaborate to combat infection remain poorly characterized...
2018: PloS One
https://www.readbyqxmd.com/read/29676724/wolbachia-mediated-protection-of-drosophila-melanogaster-against-systemic-infection-with-its-natural-viral-pathogen-drosophila-c-virus-does-not-involve-changes-in-levels-of-highly-abundant-mirnas
#18
Verna Monsanto-Hearne, Karyn N Johnson
The presence of Wolbachia confers virus protection to insects. The molecular mechanism underlying Wolbachia-mediated protection in this tripartite host-endosymbiont-virus interaction is not yet fully understood. In the bipartite association between Drosophila melanogaster and Drosophila C virus (DCV), changes in the expression of microRNAs (miRNAs) influence the outcome of viral pathogenesis. Here we examined whether changes in miRNA expression are similarly involved in the Drosophila-Wolbachia-DCV association...
April 20, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29675003/role-of-micrornas-and-exosomes-in-helicobacter-pylori-and-epstein-barr-virus-associated-gastric-cancers
#19
REVIEW
Iva Polakovicova, Sofia Jerez, Ignacio A Wichmann, Alejandra Sandoval-Bórquez, Nicolás Carrasco-Véliz, Alejandro H Corvalán
Emerging evidence suggests that chronic inflammation caused by pathogen infection is connected to the development of various types of cancer. It is estimated that up to 20% of all cancer deaths is linked to infections and inflammation. In gastric cancer, such triggers can be infection of the gastric epithelium by either Helicobacter pylori ( H. pylori ), a bacterium present in half of the world population; or by Epstein-Barr virus (EBV), a double-stranded DNA virus which has recently been associated with gastric cancer...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29673190/the-role-of-mirnas-in-virus-mediated-oncogenesis
#20
REVIEW
Zuzana Vojtechova, Ruth Tachezy
To date, viruses are reported to be responsible for more than 15% of all tumors worldwide. The oncogenesis could be influenced directly by the activity of viral oncoproteins or by the chronic infection or inflammation. The group of human oncoviruses includes Epstein–Barr virus (EBV), human papillomavirus (HPV), hepatitis B virus (HBV), hepatitis C virus (HCV), human herpesvirus 8 (HHV-8) or polyomaviruses, and transregulating retroviruses such as HIV or HTLV-1. Most of these viruses express short noncoding RNAs called miRNAs to regulate their own gene expression or to influence host gene expression and thus contribute to the carcinogenic processes...
April 17, 2018: International Journal of Molecular Sciences
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