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https://www.readbyqxmd.com/read/28938133/genetic-polymorphisms-for-19-x-str-loci-of-sichuan-han-ethnicity-and-its-comparison-with-chinese-populations
#1
Guanglin He, Ye Li, Xing Zou, Mengge Wang, Pengyu Chen, Miao Liao, Jin Wu
X-chromosomal short tandem repeats (X-STRs) can be serviced as a complementary tool in forensic deficiency cases and other complicated kinship identification. To investigate the genetic variation of the western Chinese Han population, genetic polymorphisms of 19 X-STR loci (DXS8378, DXS7423, DXS10148, DXS10159, DXS10134, DXS7424, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103, DXS10101, HPRTB, DXS6809, DXS10075, DXS10074 and DXS10135) included in the AGCU X19 PCR amplification kit were obtained from 201 Chinese Han individuals (108 females and 93 males) residing in Sichuan province, western China...
September 15, 2017: Legal Medicine
https://www.readbyqxmd.com/read/28938018/genome-wide-identification-of-bacterial-plant-colonization-genes
#2
Benjamin J Cole, Meghan E Feltcher, Robert J Waters, Kelly M Wetmore, Tatiana S Mucyn, Elizabeth M Ryan, Gaoyan Wang, Sabah Ul-Hasan, Meredith McDonald, Yasuo Yoshikuni, Rex R Malmstrom, Adam M Deutschbauer, Jeffery L Dangl, Axel Visel
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system...
September 22, 2017: PLoS Biology
https://www.readbyqxmd.com/read/28937846/polybrominated-diphenyl-ethers-pbdes-in-floor-and-road-dust-from-a-manual-e-waste-dismantling-facility-and-adjacent-communities-in-thailand
#3
Dudsadee Muenhor, Hyo-Bang Moon, Sunggyu Lee, Emma Goosey
This study characterizes concentrations of polybrominated diphenyl ethers (PBDEs) in floor and road dust from a manual e-waste dismantling facility and adjacent communities in Thailand. Levels of Σ22 PBDEs in floor dust from the facility varied between 1,200 and 43,000 ng g(-1), whereas those from adjacent communities were in the range 6.6-2,200 ng g(-1). Concentrations of Σ22 PBDEs (43,000 ng g(-1)) were highest in floor dust collected from the facility. Levels of Σ22 PBDEs and all congeners studied, except for BDE-66, BDE-71, BDE-85, BDE-119, BDE-138, BDE-190 and BDE-191 in facility dust were significantly greater than those in residential dust (P = <0...
September 22, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28937513/entrustment-evidence-used-by-expert-gynecologic-surgical-teachers-to-determine-residents-autonomy
#4
Xiaodong Phoenix Chen, Amy M Sullivan, Joan M Bengtson, John L Dalrymple
OBJECTIVE: To identify entrustment evidence used by expert gynecologic surgical teachers to determine obstetrics and gynecology residents' level of autonomy in the operating room. METHODS: A qualitative interview study was undertaken from March to November 2016. Four selection criteria were used to define and purposefully sample expert gynecologic surgical teachers across the United States to represent all four geographic regions. All interviews were audio-recorded and transcribed...
October 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28936545/open-tracheostomy-training-a-nationwide-survey-among-otolaryngology-head-and-neck-surgery-residents
#5
Limor Muallem-Kalmovich, Jacob Pitaro, Ayman Asaly, Alex Kessler, Ephraim Eviatar, Moran Shteiner, Tal Marom
The aim of this study was to examine the training methods and needs of Otolaryngology-Head and Neck Surgery (OTO-HNS) residents to independently perform open tracheostomy (OT). An anonymous 26-items questionnaire pertaining to OT teaching aspects was distributed to all 93 Israeli OTO-HNS residents during March-June 2016. Residents were categorized as 'juniors,' if they were in their post-graduate year (PGY)-1 and PGY-2; 'mid-residency' (PGY-3 and PGY-4); or 'seniors' (PGY-5 and PGY-6). Response rate was 74% (n = 69)...
September 21, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28935862/unusual-interlayer-quantum-transport-behavior-caused-by-the-zeroth-landau-level-in-ybmnbi2
#6
J Y Liu, J Hu, D Graf, T Zou, M Zhu, Y Shi, S Che, S M A Radmanesh, C N Lau, L Spinu, H B Cao, X Ke, Z Q Mao
Relativistic fermions in topological quantum materials are characterized by linear energy-momentum dispersion near band crossing points. Under magnetic fields, relativistic fermions acquire Berry phase of π in cyclotron motion, leading to a zeroth Landau level (LL) at the crossing point, a signature unique to relativistic fermions. Here we report the unusual interlayer quantum transport behavior resulting from the zeroth LL mode observed in the time reversal symmetry breaking type II Weyl semimetal YbMnBi2...
September 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28935649/melanoma-sequentially-suppresses-different-dc-subsets-in-the-sentinel-lymph-node-affecting-disease-spread-and-recurrence
#7
Mari F C M van den Hout, Bas D Koster, Berbel J R Sluijter, Barbara G Molenkamp, Rieneke van de Ven, Alfons J M van den Eertwegh, Rik J Scheper, Paul A van Leeuwen, M Petrousjka van den Tol, Tanja D de Gruijl
Melanoma exerts immune suppressive effects to facilitate tumor progression and metastatic spread. We studied these effects on dendritic cell (DC) and T-cell subsets in 36 melanoma sentinel lymph nodes (SLN) from 28 stage I-III melanoma patients and determined their clinical significance. Four conventional DC subsets; plasmacytoid DCs; and CD4+, CD8+, and regulatory T cells (Tregs) were analyzed by flow cytometry. We correlated these data to clinical parameters and determined their effect on local and distant melanoma recurrence, with a median follow-up of 75 months...
September 21, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/28935639/isolation-and-characterization-of-endothelial-to-mesenchymal-transition-cells-in-pulmonary-arterial-hypertension
#8
Toshio Suzuki, Erica J Carrier, Megha A Talati, Anandharajan Rathinasabapathy, Xinping Chen, Rintaro Nishimura, Yuji Tada, Koichiro Tatsumi, James D West
Endothelial-to-mesenchymal transition (EndMT) is a process in which endothelial cells lose polarity and cell-to-cell contacts, and undergo a dramatic remodeling of the cytoskeleton. It has been implicated in initiation and progression of pulmonary arterial hypertension (PAH). However, the characteristics of cells which have undergone EndMT-cells in vivo have not been reported and so remain unclear. To study this, sugen5416 and hypoxia (SuHx)-induced PAH was established in Cdh5-Cre / Gt(ROSA)26Sor(tm4(ACTB-tdTomato,-EGFP)Luo)/J double-transgenic mice, in which GFP was stably expressed in pan-endothelial cells...
September 21, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28934343/disparate-gain-and-loss-of-parasitic-abilities-among-nematode-lineages
#9
Martijn Holterman, Akbar Karegar, Paul Mooijman, Hanny van Megen, Sven van den Elsen, Mariette T W Vervoort, Casper W Quist, Gerrit Karssen, Wilfrida Decraemer, Charles H Opperman, David M Bird, Jan Kammenga, Aska Goverse, Geert Smant, Johannes Helder
Plant parasitism has arisen time and again in multiple phyla, including bacteria, fungi, insects and nematodes. In most of these organismal groups, the overwhelming diversity hampers a robust reconstruction of the origins and diversification patterns of this trophic lifestyle. Being a moderately diversified phylum with ≈ 4,100 plant parasites (15% of total biodiversity) subdivided over four independent lineages, nematodes constitute a major organismal group for which the genesis of plant parasitism could be mapped...
2017: PloS One
https://www.readbyqxmd.com/read/28934245/angiopoietin-like-protein-2-regulates-porphyromonas-gingivalis-lipopolysaccharide-induced-inflammatory-response-in-human-gingival-epithelial-cells
#10
Tasuku Ohno, Genta Yamamoto, Jun-Ichiro Hayashi, Eisaku Nishida, Hisashi Goto, Yasuyuki Sasaki, Takeshi Kikuchi, Mitsuo Fukuda, Yoshiaki Hasegawa, Makio Mogi, Akio Mitani
Angiopoietin-like protein 2 (ANGPTL2) maintains tissue homeostasis by inducing inflammation and angiogenesis. It is produced in infiltrating immune cells or resident cells, such as adipocytes, vascular endothelial cells, and tumor cells. We hypothesized that ANGPTL2 might play an important role as a unique mediator in both systemic and periodontal disease. We demonstrated an increased ANGPTL2 concentration in gingival crevicular fluid from chronic periodontitis patients. Porphyromonas gingivalis lipopolysaccharide (LPS) treatment strongly induced ANGPTL2 mRNA and protein levels in Ca9-22 human gingival epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28934184/rural-and-urban-differences-in-passenger-vehicle-occupant-deaths-and-seat-belt-use-among-adults-united-states-2014
#11
Laurie F Beck, Jonathan Downs, Mark R Stevens, Erin K Sauber-Schatz
PROBLEM/CONDITION: Motor-vehicle crashes are a leading cause of death in the United States. Compared with urban residents, rural residents are at an increased risk for death from crashes and are less likely to wear seat belts. These differences have not been well described by levels of rurality. REPORTING PERIOD: 2014. DESCRIPTION OF SYSTEMS: Data from the Fatality Analysis Reporting System (FARS) and the Behavioral Risk Factor Surveillance System (BRFSS) were used to identify passenger-vehicle-occupant deaths from motor-vehicle crashes and estimate the prevalence of seat belt use...
September 22, 2017: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
https://www.readbyqxmd.com/read/28933734/growth-hormone-s-effect-on-adipose-tissue-quality-versus-quantity
#12
REVIEW
Darlene E Berryman, Edward O List
Obesity is an excessive accumulation or expansion of adipose tissue (AT) due to an increase in either the size and/or number of its characteristic cell type, the adipocyte. As one of the most significant public health problems of our time, obesity and its associated metabolic complications have demanded that attention be given to finding effective therapeutic options aimed at reducing adiposity or the metabolic dysfunction associated with its accumulation. Growth hormone (GH) has therapeutic potential due to its potent lipolytic effect and resultant ability to reduce AT mass while preserving lean body mass...
July 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28933066/multiple-sclerosis-implications-of-obesity-in-neuroinflammation
#13
Ana Margarida Novo, Sónia Batista
Since the discovery of the remarkable properties of adipose tissue as a metabolically active organ, several evidences on the possible link between obesity and the pathogenesis of multiple sclerosis (MS) have been gathered. Obesity in early life, mainly during adolescence, has been proposed as a relevant risk factor for late MS development. Moreover, once MS is initiated, obesity can contribute to increase disease severity by negatively influencing disease progress. Despite the fact that clinical data are not yet conclusive, many biochemical links have been recently disclosed...
2017: Advances in Neurobiology
https://www.readbyqxmd.com/read/28932871/crystal-structures-of-eukaryote-glycosyltransferases-reveal-biologically-relevant-enzyme-homooligomers
#14
REVIEW
Deborah Harrus, Sakari Kellokumpu, Tuomo Glumoff
Glycosyltransferases (GTases) transfer sugar moieties to proteins, lipids or existing glycan or polysaccharide molecules. GTases form an important group of enzymes in the Golgi, where the synthesis and modification of glycoproteins and glycolipids take place. Golgi GTases are almost invariably type II integral membrane proteins, with the C-terminal globular catalytic domain residing in the Golgi lumen. The enzymes themselves are divided into 103 families based on their sequence homology. There is an abundance of published crystal structures of GTase catalytic domains deposited in the Protein Data Bank (PDB)...
September 20, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28932248/opportunities-for-promoting-physical-activity-in-rural-communities-by-understanding-the-interests-and-values-of-community-members
#15
Thomas Park, Amy A Eyler, Rachel G Tabak, Cheryl Valko, Ross C Brownson
PURPOSE: Physical activity (PA) has well-established health benefits, but most Americans do not meet national guidelines. In southeastern Missouri, trails have been developed to increase rates of PA. Although this has had success, broad-scale interventions will be needed to improve rates further. In this study, we surveyed residents of southeastern Missouri to identify ways to improve rates of PA. METHODS: We conducted a telephone survey in 2015 of adults (n = 524) from eight rural Missouri towns that had walking trails, regarding their activities and interests...
2017: Journal of Environmental and Public Health
https://www.readbyqxmd.com/read/28931648/targeting-epithelial-mesenchymal-plasticity-in-cancer-clinical-and-preclinical-advances-in-therapy-and-monitoring
#16
REVIEW
Sugandha Bhatia, James Monkman, Alan Kie Leong Toh, Shivashankar H Nagaraj, Erik W Thompson
The concept of epithelial-mesenchymal plasticity (EMP), which describes the dynamic flux within the spectrum of phenotypic states that invasive carcinoma cells may reside, is being increasingly recognised for its role in cancer progression and therapy resistance. The myriad of events that are able to induce EMP, as well as the more recently characterised control loops, results in dynamic transitions of cancerous epithelial cells to more mesenchymal-like phenotypes through an epithelial-mesenchymal transition (EMT), as well as the reverse transition from mesenchymal phenotypes to an epithelial one...
September 20, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28931056/heterogeneity-in-the-expression-and-subcellular-localization-of-polyol-monosaccharide-transporter-genes-in-lotus-japonicus
#17
Lu Tian, Leru Liu, Yehu Yin, Mingchao Huang, Yanbo Chen, Xinlan Xu, Pingzhi Wu, Meiru Li, Guojiang Wu, Huawu Jiang, Yaping Chen
Polyols can serve as a means for the translocation of carbon skeletons and energy between source and sink organs as well as being osmoprotective solutes and antioxidants which may be involved in the resistance of some plants to biotic and abiotic stresses. Polyol/Monosaccharide transporter (PLT) proteins previously identified in plants are involved in the loading of polyols into the phloem and are reported to be located in the plasma membrane. The functions of PLT proteins in leguminous plants are not yet clear...
2017: PloS One
https://www.readbyqxmd.com/read/28930762/competency-based-medical-education-in-the-internal-medicine-clerkship-a-report-from-the-alliance-for-academic-internal-medicine-undergraduate-medical-education-task-force
#18
Sara B Fazio, Cynthia H Ledford, Paul B Aronowitz, Shobhina G Chheda, John H Choe, Stephanie A Call, Scott D Gitlin, Marty Muntz, L James Nixon, Anne G Pereira, John W Ragsdale, Emily A Stewart, Karen E Hauer
As medical educators continue to redefine learning and assessment across the continuum, implementation of competency-based medical education in the undergraduate setting has become a focus of many medical schools. While standards of competency have been defined for the graduating student, there is no uniform approach for defining competency expectations for students during their core clerkship year. The authors describe the process by which an Alliance for Academic Internal Medicine task force developed a paradigm for competency-based assessment of students during their inpatient internal medicine (IM) clerkship...
September 14, 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/28930091/proposal-to-assess-printability-of-bioinks-for-extrusion-based-bioprinting-and-evaluation-of-rheological-properties-governing-bioprintability
#19
Naomi Claire Paxton, Willi Smolan, Thomas Böck, Ferry P W Melchels, Juergen Groll, Tomasz Juengst
The development and formulation of printable inks for extrusion-based 3D bioprinting has been a major challenge in the field of biofabrication. Inks, often polymer solutions with the addition of crosslinking to form hydrogels, must not only display adequate mechanical properties for the chosen application, but also show high biocompatibility as well as printability. Here we describe a reproducible two-step method for the assessment of the printability of inks for bioprinting, focussing firstly on screening ink formulations to assess fibre formation and the ability to form 3D constructs before presenting a method for the rheological evaluation of inks to characterise the yield point, shear thinning and recovery behaviour...
September 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28929933/the-extent-and-type-of-gambling-harms-for-concerned-significant-others-a-cross-sectional-population-study-in-finland
#20
Anne H Salonen, Hannu Alho, Sari Castrén
AIMS: This study investigates the proportion of concerned significant others (CSOs) of problem gamblers at population level and describes the extent and type of gambling harms for CSOs. METHODS: Cross-sectional random sample data ( n = 4515) were collected in 2015. The data were weighted based on age, gender and residence. CSOs were identified using a question including seven options. Gambling harms were inquired using structured questions. Descriptive statistics and Chi-Squared and Fischer's exact tests were used...
December 2016: Scandinavian Journal of Public Health
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