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https://www.readbyqxmd.com/read/29684854/the-role-of-her2-nrf2-axis-in-induction-of-oxaliplatin-resistance-in-colon-cancer-cells
#1
Abbas Pirpour Tazehkand, Maryam Akbarzadeh, Kobra Velaie, Mohammad Reza Sadeghi, Nasser Samadi
Nuclear factor erythroid 2-related factor 2 (Nrf2) plays a pivotal role in promoting chemoresistance by regulation of antioxidants and detoxification enzymes. Her2 is a member of tyrosine kinase receptor family with a key function in resistance of cancer cells to chemotherapeutics. The aim of this study was to investigate the possible cross talk between Nrf2 and Her2 mediated signaling pathways in development of oxaliplatin resistance in colon cancer cells. We first generated oxaliplatin-resistant LS174T and SW480 colon cancer cells with different Her2 expression levels by employing IC50 concentrations followed by a resting period...
April 20, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29684684/targeted-data-independent-acquisition-for-mass-spectrometric-detection-of-ras-mutations-in-formalin-fixed-paraffin-embedded-tumor-biopsies
#2
Yeoun Jin Kim, Andrew G Chambers, Fabiola Cecchi, Todd Hembrough
Genomic testing for KRAS and NRAS mutations in clinical biopsies of various cancers is routinely performed to predict futility of anti-epidermal growth factor receptor (anti-EGFR) therapies. We hypothesized that RAS mutations could be detected and quantified at the protein level for diagnostic purposes using data-independent acquisition (DIA)-based mass spectrometry in formalin-fixed, paraffin-embedded (FFPE) tumor samples. We developed a targeted DIA assay that surveys the specific mass range of all possible peptides harboring activating mutations in KRAS exon 2...
April 20, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29684560/formulation-characteristics-and-in-vitro-release-testing-of-cyclosporine-ophthalmic-ointments
#3
Yixuan Dong, Haiou Qu, Naresh Pavurala, Jiang Wang, Vasanthakumar Sekar, Marilyn Martinez, Raafat Fahmy, Muhammad Ashraf, Celia N Cruz, Xiaoming Xu
The aim of the present study was to investigate the relationship between formulation/process variables versus the critical quality attributes (CQAs) of cyclosporine ophthalmic ointments and to explore the feasibility of using an in vitro approach to assess product sameness. A definitive screening design (DSD) was used to evaluate the impact of formulation and process variables. The formulation variables included drug percentage, percentage of corn oil and lanolin alcohol. The process variables studied were mixing temperature, mixing time and the method of mixing...
April 20, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29684519/identification-of-a-transcription-factor-that-functions-downstream-of-corazonin-in-the-control-of-desert-locust-gregarious-body-color
#4
Ryohei Sugahara, Seiji Tanaka, Akiya Jouraku, Takahiro Shiotsuki
Corazonin (Crz) is a neuropeptide that controls phase-dependent body color polyphenism in locusts. The Crz signaling pathway is responsible for the development of gregarious black patterns in nymphs and determination of the morphometric ratio F/C (F = hind femur length, C = maximum head width) in adults. However, little information is available regarding the mediator and effector proteins regulated by Crz. In this study, we identified a novel transcriptional factor, Loct, which functions downstream of Crz in Schistocerca gregaria and Locusta migratoria...
April 20, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29684412/no-association-of-il2-il4-il6-tnf-and-ifng-gene-polymorphisms-was-found-with-taenia-solium-human-infection-or-neurocysticercosis-severity-in-a-family-based-study
#5
A Fleury, C Alaez, A Dessein, M Rosetti, B Saenz, M Hernández, R J Bobes, R Aquino, E Sciutto, C Gorodezky, G Fragoso
Neurocysticercosis (NC) is caused by the establishment of the metacestode stage of Taenia solium in the human central nervous system. A great heterogeneity in the susceptibility to the infection and to the disease has been reported. While the factors involved in this heterogeneity are not completely understood, clearly different immune-inflammatory profiles have been associated to each condition. This study evaluated the association of cytokine single nucleotide polymorphisms (SNPs) with susceptibility to infection and disease severity in NC patients...
April 20, 2018: Human Immunology
https://www.readbyqxmd.com/read/29684352/nrf2-activation-protects-against-intratracheal-lps-induced-mouse-murine-acute-respiratory-distress-syndrome-by-regulating-macrophage-polarization
#6
Juan Wei, Guannan Chen, Xuan Shi, Huanping Zhou, Meiyun Liu, Yuanli Chen, Di Feng, Pengcheng Zhang, Lingmin Wu, Xin Lv
The transcription factor nuclear factor E2-related factor 2 (Nrf2) is known to control the expression of antioxidant response elements and cytoprotective genes and modulate inflammatory response, helping to ameliorate damage in many diseases. Exactly how Nrf2 regulates innate inflammatory homeostasis remains unclear. In this study, we provide in vitro and in vivo evidence that Nrf2 plays a crucial role in macrophage polarization and acute respiratory distress syndrome (ARDS). We conducted in vitro experiments using a mouse alveolar macrophage cell line as well as primary cultures of macrophages in which cells were exposed to lipopolysaccharide (LPS) or interferon-γ in order to mimic ARDS, in the presence or absence of the Nrf2 activator tert-butylhydroquinone (tBHQ)...
April 20, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29684311/tfam-is-required-for-maturation-of-the-fetal-and-adult-intestinal-epithelium
#7
Manasa Srivillibhuthur, Bailey N Warder, Natalie H Toke, Pooja P Shah, Qiang Feng, Nan Gao, Edward M Bonder, Michael P Verzi
During development, the embryo transitions from a metabolism favoring glycolysis to a metabolism favoring mitochondrial respiration. How metabolic shifts regulate developmental processes, or how developmental processes regulate metabolic shifts, remains unclear. To test the requirement of mitochondrial function in developing endoderm-derived tissues, we genetically inactivated the mitochondrial transcription factor, Tfam, using the Shh-Cre driver. Tfam mutants did not survive postnatally, exhibiting defects in lung development...
April 20, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29684038/murine-embryos-exposed-to-human-endometrial-mscs-derived-extracellular-vesicles-exhibit-higher-vegf-pdgf-aa-release-increased-blastomere-count-and-hatching-rates
#8
Rebeca Blázquez, Francisco Miguel Sánchez-Margallo, Verónica Álvarez, Elvira Matilla, Nuria Hernández, Federica Marinaro, María Gómez-Serrano, Inmaculada Jorge, Javier G Casado, Beatriz Macías-García
Endometrial Mesenchymal Stromal Cells (endMSCs) are multipotent cells with immunomodulatory and pro-regenerative activity which is mainly mediated by a paracrine effect. The exosomes released by MSCs have become a promising therapeutic tool for the treatment of immune-mediated diseases. More specifically, extracellular vesicles derived from endMSCs (EV-endMSCs) have demonstrated a cardioprotective effect through the release of anti-apoptotic and pro-angiogenic factors. Here we hypothesize that EV-endMSCs may be used as a co-adjuvant to improve in vitro fertilization outcomes and embryo quality...
2018: PloS One
https://www.readbyqxmd.com/read/29684003/17%C3%AE-estradiol-promotes-angiogenesis-of-rat-cardiac-microvascular-endothelial-cells-in-vitro
#9
HaiTao Liu, Yin Tao, Mai Chen, Jin Yu, Wei-Jie Li, Ling Tao, Yan Li, Fei Li
BACKGROUND The formation of new blood vessels, known as angiogenesis, is critical for recovery from ischemic heart disease, and estrogen is considered an important factor in this process. Here, we investigated the effects of 17β-estradiol (17β-E2) on proliferation and migration of cardiac microvascular endothelial cells (CMECs) in vitro. MATERIAL AND METHODS Rat CMECs were isolated and cultured with 17β-E2 (0.001-1 µmol/l) in the absence or presence of the estrogen antagonist tamoxifen. Then, the expression level of estrogen receptor alpha was evaluated by using immunofluorescence assay, RT-PCR, and Western blot...
April 23, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29683870/isosteviol-sodium-injection-improves-outcomes-by-modulating-tlrs-nf-%C3%AE%C2%BAb-dependent-inflammatory-responses-following-experimental-traumatic-brain-injury-in-rats
#10
Jie Zan, Hao Zhang, Min-Yi Lu, Hui-Min Beng, Kai-Lun Zhong, Xiao-Ou Sun, Wen Tan
Previous studies have shown that isosteviol sodium (STVNa) protects against permanent cerebral ischemia injury by inhibition of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-mediated inflammatory responses. Overwhelming evidence shows that toll-like receptors (TLRs) are the upstream regulators of NF-κB. On the basis of the similarity of the pathology caused by traumatic brain injury (TBI) and stroke, we speculated that STVNa may have a therapeutic effect against TBI through regulation of the TLRs/NF-κB signaling-mediated inflammatory response...
April 20, 2018: Neuroreport
https://www.readbyqxmd.com/read/29683442/genome-wide-rnai-screening-to-identify-host-factors-that-modulate-oncolytic-virus-therapy
#11
Kristina J Allan, Douglas J Mahoney, Stephen D Baird, Charles A Lefebvre, David F Stojdl
High-throughput genome-wide RNAi (RNA interference) screening technology has been widely used for discovering host factors that impact virus replication. Here we present the application of this technology to uncovering host targets that specifically modulate the replication of Maraba virus, an oncolytic rhabdovirus, and vaccinia virus with the goal of enhancing therapy. While the protocol has been tested for use with oncolytic Maraba virus and oncolytic vaccinia virus, this approach is applicable to other oncolytic viruses and can also be utilized for identifying host targets that modulate virus replication in mammalian cells in general...
April 3, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29683273/micelle-coated-hierarchically-structured-nanofibers-with-dual-release-capability-for-accelerated-wound-healing-and-infection-control
#12
Victoria Albright, Meng Xu, Anbazhagan Palanisamy, Jun Cheng, Mary Stack, Beilu Zhang, Arul Jayaraman, Svetlana A Sukhishvili, Hongjun Wang
Tailoring nanofibrous matrices-a material with much promise for wound healing applications-to simultaneously mitigate bacterial colonization and stimulate wound closure of infected wounds is highly desirable. To that end, a dual-releasing, multiscale system of biodegradable electrospun nanofibers coated with biocompatible micellar nanocarriers is reported. For wound healing, transforming growth factor-β1 is incorporated into polycaprolactone/collagen (PCL/Coll) nanofibers via electrospinning and the myofibroblastic differentiation of human dermal fibroblasts is locally stimulated...
April 23, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29683032/applicability-of-api-zym-to-capture-seasonal-and-spatial-variabilities-in-lake-and-river-sediments
#13
Drashti Patel, Renee Gismondi, Ali Alsaffar, Sonia M Tiquia-Arashiro
Waters draining into a lake carry with them much of the suspended sediment that is transported by rivers and streams from the local drainage basin. The organic matter processing in the sediments is executed by heterotrophic microbial communities, whose activities may vary spatially and temporally. Thus, to capture and evaluate some of these variabilities in the sediments, we sampled six sites: three from St. Clair River (SC1, SC2, and SC3) and three from Lake St. Clair in spring, summer, fall and winter of 2016...
April 21, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29682557/alpha-l-fucosidase-serves-as-a-prognostic-indicator-for-intrahepatic-cholangiocarcinoma-and-inhibits-its-invasion-capacity
#14
Zeyu Shuang, Yize Mao, Guohe Lin, Jun Wang, Xin Huang, Jianlin Chen, Fangting Duan, Shengping Li
Alpha-L-fucosidase (AFU) has been reported to be a predictor of survival in patients with several cancers, but it is unclear whether AFU is associated with prognosis in patients with intrahepatic cholangiocarcinoma (iCCA). In this study, we used receiver operating characteristic (ROC) analysis to generate the cutoff point of AFU for overall survival (OS). The prognostic influence of the AFU level in serum on OS was studied using Kaplan-Meier curves. Moreover, invasion assays and Western blotting were performed to explore the effects of AFU on iCCA invasion in vitro...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29682545/curcumin-as-a-promising-antibacterial-agent-effects-on-metabolism-and-biofilm-formation-in-s-mutans
#15
Bingchun Li, Xinlong Li, Huancai Lin, Yan Zhou
Streptococcus mutans (S. mutans) has been proved to be the main aetiological factor in dental caries. Curcumin, a natural product, has been shown to exhibit therapeutic antibacterial activity, suggesting that curcumin may be of clinical interest. The objective of this study is to evaluate the inhibitory effects of curcumin on metabolism and biofilm formation in S. mutans using a vitro biofilm model in an artificial oral environment. S. mutans biofilms were treated with varying concentrations of curcumin. The biofilm metabolism and biofilm biomass were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the crystal violet assay...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29682535/the-role-of-mirna-132-against-apoptosis-and-oxidative-stress-in-heart-failure
#16
Xuelei Liu, Zhou Tong, Keyan Chen, Xiaofang Hu, Hongxu Jin, Mingxiao Hou
Objective: To explore the effect of microRNA-132 of heart failure and provide theoretical guidance for clinical treatment of heart failure (HF). Methods: Peripheral blood was collected from HF patients. RT-qPCR was used to determine microRNA-132 expression. Mouse models of heart failure were established. Color Doppler ultrasound was utilized to measure the changes of cardiac function. HE and Masson staining were applied to observe pathological changes of the myocardium...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29682351/comparative-analysis-of-cellular-and-growth-factor-composition-in-bone-marrow-aspirate-concentrate-and-platelet-rich-plasma
#17
Hisashi Sugaya, Tomokazu Yoshioka, Toshiki Kato, Yu Taniguchi, Hiroshi Kumagai, Kojiro Hyodo, Osamu Ohneda, Masashi Yamazaki, Hajime Mishima
The purpose of this study was to quantify the stem cell and growth factor (GF) contents in the bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) prepared from whole blood using a protocol established in our laboratory. We examined 10 patients with osteonecrosis of the femoral head who were treated by autologous BMAC transplantation at our hospital between January 2015 and June 2015. We quantified CD34+ and CD31-CD45-CD90+CD105+ cells in BMAC and PRP by flow cytometry. Additionally, we measured various GFs, that is, basic fibroblast growth factor (b-FGF), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor (VEGF), transforming growth factor- β 1 (TGF- β 1), and bone morphogenetic protein-2 (BMP-2) in BMAC and PRP using enzyme-linked immunosorbent assays and statistical analyses...
2018: Bone Marrow Research
https://www.readbyqxmd.com/read/29682203/mutation-analysis-of-the-egfr-pathway-genes-egfr-ras-pik3ca-braf-and-akt1-in-salivary-gland-adenoid-cystic-carcinoma
#18
Kosuke Saida, Takayuki Murase, Mayuko Ito, Kana Fujii, Hisashi Takino, Ayako Masaki, Daisuke Kawakita, Kei Ijichi, Yuichiro Tada, Kimihide Kusafuka, Yoshiyuki Iida, Tetsuro Onitsuka, Yasushi Yatabe, Nobuhiro Hanai, Yasuhisa Hasegawa, Hitomi Shinomiya, Ken-Ichi Nibu, Kazuo Shimozato, Hiroshi Inagaki
Adenoid cystic carcinoma (AdCC), one of the most common salivary gland carcinomas, usually has a fatal outcome. Epidermal growth factor receptor (EGFR) pathway gene mutations are important in predicting a patient's prognosis and estimating the efficacy of molecular therapy targeting the EGFR pathway. In this study of salivary gland AdCC (SAdCC), we looked for gene mutations in EGFR, RAS family ( KRAS, HRAS, and NRAS ), PIK3CA, BRAF, and AKT1 , using a highly sensitive single-base extension multiplex assay, SNaPshot...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29682178/prognostic-value-of-circulating-micrornas-in-upper-tract-urinary-carcinoma
#19
Ruth Montalbo, Laura Izquierdo, Mercedes Ingelmo-Torres, Juan José Lozano, David Capitán, Antonio Alcaraz, Lourdes Mengual
The identification of upper tract urinary carcinoma (UTUC) prognostic biomarkers is urgently needed to predict tumour progression. This study aimed to identify serum microRNAs (miRNAs) that may be useful as minimally invasive predictive biomarkers of tumour progression and survival in UTUC patients. To this end, 33 UTUC patients who underwent radical nephroureterectomy at the Hospital Clinic of Barcelona were prospectively included. Expression of 800 miRNAs was evaluated in serum samples from these patients using nCounter® miRNA Expression Assays...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29682177/chemerin-induces-endothelial-cell-inflammation-activation-of-nuclear-factor-kappa-beta-and-monocyte-endothelial-adhesion
#20
Georgios K Dimitriadis, Jaspreet Kaur, Raghu Adya, Alexander D Miras, Harman S Mattu, John G Hattersley, Gregory Kaltsas, Bee K Tan, Harpal S Randeva
Chemerin, a chemoattractant protein, acts via a G-protein coupled chemokine receptor, i.e. Chemokine like Receptor 1/ChemR23; levels of which are elevated in pro-inflammatory states such as obesity and type 2 diabetes mellitus (T2DM). Obesity and T2DM patients are at high risk of developing cardiovascular disorders such as atherosclerosis. We have reported that chemerin induces human endothelial cell angiogenesis and since dysregulated angiogenesis and endothelial dysfunction are hallmarks of vascular disease; we sought to determine the effects of chemerin on monocyte-endothelial adhesion, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a critical pro-inflammatory transcription factor...
March 30, 2018: Oncotarget
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