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https://www.readbyqxmd.com/read/29245068/overexpression-of-microrna-375-impedes-platelet-derived-growth-factor-induced-proliferation-and-migration-of-human-fetal-airway-smooth-muscle-cells-by-targeting-janus-kinase-2
#1
Yamei Ji, Xin Yang, Huixia Su
The abnormal proliferation and migration of airway smooth muscle (ASM) cells play a critical role in airway remodeling during the development of asthma. MicroRNAs (miRNAs) have emerged as critical regulators of ASM cell proliferation and migration in airway remodeling. In this study, we aimed to investigate the potential role of miR-375 in the regulation of platelet-derived growth factor (PDGF)-induced fetal ASM cell proliferation and migration. Our results showed that miR-375 expression was significantly decreased in fetal ASM cells that were treated with PDGF...
December 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29244999/mechanical-stress-increases-brain-amyloid-%C3%AE-tau-and-%C3%AE-synuclein-concentrations-in-wild-type-mice
#2
Marcel Levy Nogueira, Minoo Hamraz, Mohammad Abolhassani, Erwan Bigan, Olivier Lafitte, Jean-Marc Steyaert, Bruno Dubois, Laurent Schwartz
INTRODUCTION: Exposure to traumatic brain injury is a core risk factor that predisposes an individual to sporadic neurodegenerative diseases. We provide evidence that mechanical stress increases brain levels of hallmark proteins associated with neurodegeneration. METHODS: Wild-type mice were exposed to multiple regimens of repetitive mild traumatic brain injury, generating a range of combinations of impact energies, frequencies, and durations of exposure. Brain concentrations of amyloid β 1-42 (Aβ1-42), total tau, and α-synuclein were measured by sandwich enzyme-linked immunosorbent assay...
December 12, 2017: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
https://www.readbyqxmd.com/read/29244789/pigment-epithelium-derived-factor-from-arpe19-promotes-proliferation-and-inhibits-apoptosis-of-human-umbilical-mesenchymal-stem-cells-in-serum-free-medium
#3
Dah-Ching Ding, Yao-Tseng Wen, Rong-Kung Tsai
Clinical expansion of mesenchymal stem cells (MSCs) is hampered by the lack of knowledge regarding how to prevent MSC apoptosis and promote their proliferation in serum-free medium. Our in vitro studies demonstrated that human umbilical cord MSCs (HUCMSCs) underwent apoptosis in the serum-free medium. When HUCMSCs were co-cultured with retinal pigment epithelial cells (ARPE19), however, HUCMSCs exhibited normal growth and morphology in serum-free medium. Their colony formation was promoted by the conditioned medium (CM) of ARPE19 cells on Matrigel...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29244610/inhibition-of-exosome-release-by-ketotifen-enhances-sensitivity-of-cancer-cells-to-doxorubicin
#4
Farman Matloob Khan, Ekram Saleh, Hussain Alawadhi, Rania Harati, Wolfram-Hubertus Zimmermann, Raafat El-Awady
Exosomes released from cancer cells support metastasis and growth of recipient cells and increase their resistance to chemotherapy. Therapeutic targeting of exosomes is a promising area in cancer research. Our aim is to test the effect of the mast cell stabilizer ketotifen on exosomes release from cancer cells and how this can modify their response to doxorubicin. Exosomes release from three cancer cell lines (MCF7, HeLa and BT549) was assessed by scan electron microscope and exosomes quantification kit. Doxorubicin export within exosomes was monitored flurometrically and cellular sensitivity to doxorubicin ± ketotifen was measured by sulphorhodamine-B and colony formation assays...
December 15, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29243822/nrf2-promotes-oesophageal-cancer-cell-proliferation-via-metabolic-reprogramming-and-detoxification-of-reactive-oxygen-species
#5
Yuki Kitano, Yoshifumi Baba, Shigeki Nakagawa, Keisuke Miyake, Masaaki Iwatsuki, Takatsugu Ishimoto, Yo-Ichi Yamashita, Naoya Yoshida, Masayuki Watanabe, Mitsuyoshi Nakao, Hideo Baba
Cancer cells consume a large amount of energy and maintain high levels of anabolism to promote cell proliferation via metabolic reprogramming. Nuclear factor erythroid 2-related factor 2 (Nrf2; NFE2L2) is a master transcription regulator of stress responses and promotes metabolic reprogramming to support cell proliferation in various types of cancer. As oesophageal cancer is one of the most aggressive gastrointestinal cancers, we aimed to clarify the effect of Nrf2 on metabolic reprogramming in oesophageal cancer...
December 15, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/29243793/a-new-antioxidant-formulation-reduces-the-apoptotic-and-damaging-effect-of-cigarette-smoke-extract-on-human-bronchial-epithelial-cells
#6
L Vanella, G Li Volti, A Distefano, M Raffaele, V Zingales, R Avola, D Tibullo, I Barbagallo
OBJECTIVE: In this study we evaluated the possible protective effect of an antioxidant formulation containing microfiltered milk derived polypeptides, Curcumin, Vitamin B2, Carnitine and N-Acetyl-cysteine (NAC) in an in vitro model of chronic obstructive pulmonary disease (COPD). MATERIALS AND METHODS: Human bronchial epithelial cells (16HBE) were used in this study. Cells were treated for 24 h in the presence or absence of 10% of cigarette smoke extract (CSE) and in the presence or absence of antioxidant formulation...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29243790/mir-93-inhibition-ameliorates-ogd-r-induced-cardiomyocyte-apoptosis-by-targeting-nrf2
#7
L-J Yan, X-W Fan, H-T Yang, J-T Wu, S-L Wang, C-G Qiu
OBJECTIVE: To identify the expression changes of microRNA 93 (miR-93) in oxygen-glucose deprivation/reoxygenation (OGD/R) injury in cardiomyocytes and its mechanism of mediating OGD/R and inducing apoptosis. MATERIALS AND METHODS: Primary cardiomyocytes were extracted and OGD/R model in cardiomyocytes was established in vitro. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expressions of miR-93, and Western blot assay was applied to measure the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and caspase-3...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29243776/hypoxia-promotes-invasion-of-retinoblastoma-cells-in-vitro-by-upregulating-hif-1%C3%AE-mmp9-signaling-pathway
#8
Y-Y Li, Y-L Zheng
OBJECTIVE: The aim of this study is to investigate the effect of hypoxia-inducible transcription factors-1α (HIF-1α)/matrix metalloproteinase 9 (MMP9) signaling pathway on hypoxia triggered invasion in retinoblastoma cell line HXO-RB44. MATERIALS AND METHODS: HXO-RB44 cells were cultured under hypoxia conditions for 24 h. The effect of hypoxia on invasion ability of HXO-RB44 cells was monitored with transwell invasion assay; the mRNA and protein expression levels of HIF-1α and MMP9 were detected by Real-time polymerase chain reaction (RT-PCR) and Western blot; luciferase assay was performed to assess the MMP9 regulation by HIF-1α, and HIF-1α regulation by hypoxia...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29243775/independent-prognostic-factor-of-low-expressed-lncrna-znf667-as1-for-cervical-cancer-and-inhibitory-function-on-the-proliferation-of-cervical-cancer
#9
L-P Zhao, R-H Li, D-M Han, X-Q Zhang, G-X Nian, M-X Wu, Y Feng, L Zhang, Z-G Sun
OBJECTIVE: To investigate the expression of long non-coding RNA ZNF667-AS1 in cervical cancer and its effect on the proliferation of cervical cancer cell line, SiHa cells. MATERIALS AND METHODS: The expression level of ZNF667-AS1 from two microarray datasets (GSE63514 and GSE6791) and TCGA (The Cancer Genome Atlas) were selected to analyze the difference between cervical cancer tissues and normal cervical tissues with bioinformatics methods. Then, the prognosis of ZNF667-AS1 was calculated in TCGA...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29243437/-intervention-effects-of-zuoguiwan-containing-serum-on-osteoblast-through-erk1-2-and-wnt-%C3%AE-catenin-signaling-pathway-in-models-with-kidney-yang-deficiency-kidney-yin-deficiency-osteoporosis-syndromes
#10
Jian-Hua Zhang, Jing Xin, Lian-Xia Fan, Hua Yin
To clarify the effects of Zuoguiwan containing serum on osteoblast proliferation and alkaline phosphatase(ALP) expression and its effects on the expression of β-catenin, ERK1, ERK2 mRNA and protein of osteoblast through ERK1/2, Wnt/β-catenin signaling pathway in models with osteoporosis(OP) kidney-Yang-deficiency, osteoporosis(OP) kidney-Yin-deficiency syndrome. Rat osteoporosis models were established by ovariectomy surgery, and 10 weeks after surgery, hydrocortisone was injected and thyroxine was administered by intragastric administration to establish OP kidney-Yang-deficiency rat model, and OP kidney-Yin-deficiency rat model...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29243436/-effect-of-neoflavonoid-latifolin-isolated-from-dalbergia-odorifera-on-acute-myocardial-ischemia-in-rats-and-its-mechanism-of-nrf2-signaling-pathway
#11
Xue-Liang Li, Lan-Ying Chen, Zi-Yi Guan, Yao Luo, Ya-Ru Cui, Rong-Hua Liu, Feng Shao, Ding-Qing Wang
The present study was designed to evaluate the cardioprotective effect of latifolin on pituitrin(Pit) or isoproterenol(ISO)-induced myocardial injury in rats, and further investigate its underlying mechanisms. Rats were administrated sublingually with pituitrin or subcutaneously with isoproterenol to induce acute myocardial ischemia in rats, and lead II electrocardiograph was recorded. In rats with isoproterenol, ELISA assay or colorimetric method was used to detect the content or activity of myocardial injury markers in serum, and the SOD activity and MDA content in myocardium were detected by colorimetric assay; histopathological examination was conducted by HE staining; the frozen section of myocardial tissues was used for DCFH-DA fluorescent staining to detect the content of ROS in myocardium; Western blot was used to detect the protein expression levels of Nrf2, Keap1, HO-1 and NQO1 in myocardium...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29243406/the-national-burden-of-influenza-associated-severe-acute-respiratory-illness-hospitalization-in-zambia-2011-2014
#12
Andros Theo, Stefano Tempia, Adam L Cohen, Paul Simusika, Edward Chentulo, Chikama Mukwangole Chikamukwa, Mwaka Monze
BACKGROUND: Estimates of influenza-associated hospitalization are limited in low- and middle-income countries, especially in Africa. OBJECTIVE: To estimate the national number of influenza-associated severe acute respiratory illness (SARI) hospitalization in Zambia. METHODS: We conducted active prospective hospital-based surveillance for SARI at the University Teaching Hospital (UTH) situated in Lusaka Province during 2011-2014. Upper respiratory tract samples were tested for influenza virus using a reverse transcriptase polymerase chain reaction assay...
December 15, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29243243/shikonin-inhibits-myeloid-differentiation-protein-2-to-prevent-lipopolysaccharide-induced-acute-lung-injury
#13
Yali Zhang, Tingting Xu, Zheer Pan, Xiangting Ge, Chuchu Sun, Chun Lu, Hongjin Chen, Zhongxiang Xiao, Bing Zhang, Yuanrong Dai, Guang Liang
BACKGROUND AND PURPOSE: Acute lung injury (ALI) is a challenging clinical syndrome which manifests as an acute inflammatory response. Myeloid differentiation protein 2 (MD2) has an important role in mediating lipopolysaccharide (LPS)-induced inflammation. Currently, there are no effective molecular-based therapies for ALI or viable biomarkers for predicting the severity of disease. Recent preclinical studies have shown that shikonin (SHI), a natural naphthoquinone, prevents LPS-inflammation...
December 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29243196/chronic-mild-hyperhomocysteinemia-alters-inflammatory-and-oxidative-nitrative-status-and-causes-protein-dna-damage-as-well-as-ultrastructural-changes-in-cerebral-cortex-is-acetylsalicylic-acid-neuroprotective
#14
Daniella de S Moreira, Paula W Figueiró, Cassiana Siebert, Caroline A Prezzi, Francieli Rohden, Fatima C R Guma, Vanusa Manfredini, Angela T S Wyse
Homocysteine is a sulfur-containing amino acid derived from methionine metabolism. When plasma homocysteine levels exceed 10-15 μM, there is a condition known as hyperhomocysteinemia, which occur as a result of an inborn error of methionine metabolism or by non-genetic causes. Mild hyperhomocysteinemia is considered a risk factor for development of neurodegenerative diseases. The objective of the present study was to evaluate whether acetylsalicylic acid has neuroprotective role on the effect of homocysteine on inflammatory, oxidative/nitrative stress, and morphological parameters in cerebral cortex of rats subjected to chronic mild hyperhomocysteinemia...
December 14, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/29242830/interaction-of-antibiotics-with-innate-host-defense-factors-against-salmonella-enterica-serotype-newport
#15
George Sakoulas, Monika Kumaraswamy, Armin Kousha, Victor Nizet
This study examines the pharmacodynamics of antimicrobials that are used to treat Salmonella with each other and with key components of the innate immune system. Antimicrobial synergy was assessed using time-kill and checkerboard assays. Antimicrobial interactions with innate immunity were studied by employing cathelicidin LL-37, whole-blood, and neutrophil killing assays. Ceftriaxone and ciprofloxacin were found to be synergistic in vitro against Salmonella enterica serotype Newport. Ceftriaxone, ciprofloxacin, and azithromycin each demonstrated synergy with the human cathelicidin defense peptide LL-37 in killing Salmonella...
November 2017: MSphere
https://www.readbyqxmd.com/read/29242607/the-impact-of-stromal-hic-5-on-the-tumorigenesis-of-colorectal-cancer-through-lysyl-oxidase-induction-and-stromal-remodeling
#16
Tomokatsu Omoto, Joo-Ri Kim-Kaneyama, Xiao-Feng Lei, Akira Orimo, Koji Ohnishi, Kosuke Yoshihara, Aya Miyauchi, Shuo Li, Lin Gao, Takahiro Umemoto, Junichi Tanaka, Kenta Nakahara, Motohiro Takeya, Fumio Ishida, Shin-Ei Kudo, Shogo Haraguchi, Takuro Miyazaki, Akira Miyazaki
Carcinoma-associated fibroblasts (CAFs) influence tumor initiation, progression, and metastasis within the tumor-associated stroma. This suggests that CAFs would be a potential target for tumor therapy. Here we found that Hydrogen peroxide-inducible clone-5 (Hic-5), also named transforming growth factor beta-1-induced transcript 1 protein (Tgfb1i1), was strongly induced in CAFs found in human colorectal cancer. To investigate the role of Hic-5 in CAFs, we isolated CAFs and the control counterpart normal fibroblasts (NFs) from human colorectal cancer and non-cancerous regions, respectively...
December 15, 2017: Oncogene
https://www.readbyqxmd.com/read/29242385/differential-regulation-of-zfp30-expression-in-murine-airway-epithelia-through-altered-binding-of-zfp148-to-rs51434084
#17
Lucas T Laudermilk, Joseph M Thomas, Samir N Kelada
Neutrophil chemotaxis to the airways is a key aspect of host response to microbes and a feature of multiple pulmonary diseases including asthma. Tight regulation of this recruitment is critical to prevent unwanted host tissue damage and inflammation. Using a mouse (Mus musculus) model of asthma applied to the Collaborative Cross (CC), we previously identified a lung gene expression quantitative trait locus (eQTL) for Zinc finger protein 30 (Zfp30) that was also a QTL for neutrophil recruitment and the hallmark neutrophil chemokine CXCL1...
December 14, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29242270/intermedin-enlarges-the-vascular-lumen-by-inducing-the-quiescent-endothelial-cell-proliferation
#18
Li-Jun Wang, Fei Xiao, Ling-Miao Kong, De-Nian Wang, Hong-Yu Li, Yong-Gang Wei, Chun Tan, Huan Zhao, Ting Zhang, Gui-Qun Cao, Kang Zhang, Yu-Quan Wei, Han-Shuo Yang, Wei Zhang
OBJECTIVE: Intermedin plays an important role in vascular remodeling and significantly improves blood perfusion, but the precise mechanism remains unclear. Herein, we aimed to define whether vascular lumen enlargement is responsible for the intermedin-increased blood perfusion and explore the underlying cellular and molecular mechanisms. APPROACH AND RESULTS: To study the role of intermedin, we generated the IMD-KO (Adm2-/-) mice using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated 9) system...
December 14, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29242162/nf-%C3%AE%C2%BAb-signaling-is-key-in-the-wound-healing-processes-of-silk-fibroin
#19
Ye Ri Park, Md Tipu Sultan, Hyun Jung Park, Jung Min Lee, Hyung Woo Ju, Ok Joo Lee, Dong Jin Lee, David L Kaplan, Chan Hum Park
Silk fibroin (SF) is a well-studied biomaterial for tissue engineering applications including wound healing. However, the signaling mechanisms underlying the impact of SF on this phenomenon have not been determined. In this study, through microarray analysis, regulatory genes of NF-ĸB signaling were activated in SF-treated NIH3T3 cells along with other genes. Immunoblot analysis confirmed the activation of the NF-ĸB signaling pathway as SF induced protein expression levels of IKKα, IKKβ, p65, and the degradation of IκBα...
December 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29242154/high-expression-of-diffuse-panbronchiolitis-critical-region-1-gene-promotes-cell-proliferation-migration-and-invasion-in-pancreatic-ductal-adenocarcinoma
#20
Jiayi Yan, Guanghui Chen, Xuesong Zhao, Fangying Chen, Ting Wang, Fei Miao
Diffuse panbronchiolitis critical region 1 (DPCR1) is located in the major histocompatibility complex (MHC) class I. It was reported to be downregulated in invasive pituitary adenoma compared with that in non-invasive tumors, but upregulated in the precursor of gastric carcinogenesis. However, the direct effect of DPCR1 on cancer cells has rarely been reported, and the role DPCR1 in pancreatic ductal adenocarcinoma (PDAC) remains unclear. The clinical sample validation and public data analysis of the present study demonstrated that DPCR1 was upregulated markedly in PDAC and this high expression was negatively correlated with the patient prognosis...
December 11, 2017: Biochemical and Biophysical Research Communications
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