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https://www.readbyqxmd.com/read/29454091/anlotinib-inhibits-angiogenesis-via-suppressing-the-activation-of-vegfr2-pdgfr%C3%AE-and-fgfr1
#1
Binyan Lin, Xiuming Song, Dawei Yang, Dongsheng Bai, Yuyuan Yao, Na Lu
Tumor cells recruit vascular endothelial cells and circulating endothelial progenitor cells to form new vessels to support their own growth and metastasis. VEGF, PDGF-BB and FGF-2 are three major pro-angiogenic factors and applied to promote angiogenesis. In this research, we demonstrated that anlotinib, a potent multi-tyrosine kinases inhibitor (TKI), showed a significant inhibitory effect on VEGF/PDGF-BB/FGF-2-induced angiogenesis in vitro and in vivo. Wound healing assay, chamber directional migration assay and tube formation assay indicated that anlotinib inhibited VEGF/PDGF-BB/FGF-2-induced cell migration and formation of capillary-like tubes in endothelial cells...
February 14, 2018: Gene
https://www.readbyqxmd.com/read/29454023/interaction-between-hypothermia-and-delayed-mesenchymal-stem-cell-therapy-in-neonatal-hypoxic-ischemic-brain-injury
#2
Josephine Herz, Christian Köster, Barbara S Reinboth, Mark Dzietko, Wiebke Hansen, Hemmen Sabir, Cindy van Velthoven, Ivo Bendix, Ursula Felderhoff-Müser
Acute hypothermia treatment (HT) is the only clinically established intervention following neonatal hypoxic-ischemic brain injury. However, almost half of all cooled infants still die or suffer from long-lasting neurological impairments. Regenerative therapies, such as mesenchymal stem cells (MSC) appear promising as adjuvant therapy. In the present study, we hypothesized that HT combined with delayed MSC therapy results in augmented protection, improving long-term neurological outcome. Postnatal day 9 (P9) C57BL/6 mice were exposed to hypoxia-ischemia followed by 4 hours HT...
February 14, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29453937/trajectory-classes-of-cannabis-use-and-heavy-drinking-among-rural-african-american-adolescents-multilevel-predictors-of-class-membership
#3
Allen W Barton, Gene H Brody, Tamika C B Zapolski, Trenette C Goings, Steven M Kogan, Michael Windle, Tianyi Yu
AIMS: To inform research on the etiology and prevention of substance use among rural African American youth by (a) identifying developmental trajectory classes of cannabis use and heavy drinking across adolescence and young adulthood, and (b) examining associations between trajectory class membership and multi-level assessments of risk factors. DESIGN: A prospective study spanning nine years with assessments of cannabis use and heavy drinking, the catecholamines epinephrine and norepinephrine, perceived stress, and psychosocial risk factors...
February 17, 2018: Addiction
https://www.readbyqxmd.com/read/29453814/preclinical-evaluation-of-a-semi-automated-and-rapid-commercial-electrophoresis-assay-for-von-willebrand-factor-multimers
#4
Marika Pikta, Galina Zemtsovskaja, Hector Bautista, Georges Nouadje, Timea Szanto, Margus Viigimaa, Valdas Banys
BACKGROUND: The von Willebrand factor (VWF) multimer test is required to correctly subtype qualitative type 2 von Willebrand disease (VWD). The current VWF multimer assays are difficult, nonstandardized, and time-consuming. The purpose of this study was to evaluate the clinical utility of the commercial VWF multimer kit by Sebia (Lisses, France), an electrophoresis technique yielding same-day results. METHODS: Ten healthy volunteer plasma samples, in-house reference plasma (IRP) and commercial normal plasma (CNP) samples, 10 plasma samples from patients with a known VWD type, 1 hemophilia A plasma sample, and 7 external quality assurance (EQA) samples were analyzed using the commercial VWF multimer kit...
February 17, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29453688/rapid-optimization-of-spore-production-from-bacillus-amyloliquefaciens-in-submerged-cultures-based-on-dipicolinic-acid-fluorimetry-assay
#5
Hang Ren, Ya-Ting Su, Xiao-Hua Guo
Some optimization techniques have been widely applied for spore fermentation based on the plate counting. This study optimized the culture medium for the spore production of Bacillus amyloliquefaciens BS-20 and investigated the feasibility of using a dipicolonic acid (DPA) fluorimetry assay as a simpler alternative to plate counting for evaluating spore yields. Through the single-factor experiment, the metal ions and agro-industrial raw materials that significantly enhanced spore production were determined...
February 16, 2018: AMB Express
https://www.readbyqxmd.com/read/29453664/analysis-of-transactivation-potential-of-rice-oryza-sativa-l-heat-shock-factors
#6
Dhruv Lavania, Anuradha Dhingra, Anil Grover
Based on yeast one-hybrid assays, we show that the presence of C-terminal AHA motifs is not a prerequisite for transactivation potential in rice heat shock factors. Transcriptional activation or transactivation (TA) of heat stress responsive genes takes place by binding of heat shock factors (Hsfs) to heat shock elements. Analysis of TA potential of thirteen rice (Oryza sativa L.) Hsfs (OsHsfs) carried out in this study by yeast one-hybrid assay showed that OsHsfsA3 possesses strong TA potential while OsHsfs A1a, A2a, A2b, A4a, A4d, A5, A7b, B1, B2a, B2b, B2c and B4d lack TA potential...
February 16, 2018: Planta
https://www.readbyqxmd.com/read/29453609/leptin-receptor-gene-a-g-polymorphism-rs1137101-and-renal-cell-carcinoma
#7
Azza M Abdu Allah, Sally M El-Hefnway, Alshimaa M Alhanafy, Ahmed M Zahran, Heba E Kasem
Leptin plays an important role in carcinogenesis as leptin/leptin receptor signaling promotes the angiogenesis, proliferation, and inhibits epithelial cell apoptosis. Variants in the leptin receptor gene have potential associations with renal cell carcinoma (RCC). We aimed to investigate association of rs1137101 (A/G) polymorphism at LEPR gene with risk of RCC and patients survival. 123 individuals were classified into group I: 73 RCC patients and group II: 50 healthy controls. Genotyping of the Gln223Arg (A/G) polymorphism rs1137101 at LEPR gene was analyzed using allelic discrimination assay by Real-Time PCR technique...
February 16, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29453576/monitoring-the-integrity-of-the-blood-testis-barrier-btb-an-in-vivo-assay
#8
Haiqi Chen, Wing-Yee Lui, Dolores D Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian, Will M Lee, Bruno Silvestrini, C Yan Cheng
The blood-testis barrier is a unique ultrastructure in the mammalian testis, located near the basement membrane of the seminiferous tubule that segregates the seminiferous epithelium into the basal and the adluminal (apical) compartment. Besides restricting paracellular and transcellular passage of biomolecules (e.g., paracrine factors, hormones), water, electrolytes, and other substances including toxicants and/or drugs to enter the adluminal compartment of the epithelium, the BTB is an important ultrastructure that supports spermatogenesis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29453407/neovasculature-can-be-induced-by-patching-an-arterial-graft-into-a-vein-a-novel-in-vivo-model-of-spontaneous-arteriovenous-fistula-formation
#9
Yukinobu Ito, Makoto Yoshida, Daichi Maeda, Masato Takahashi, Hiroshi Nanjo, Hirotake Masuda, Akiteru Goto
Arteriovenous malformations consist of tangles of arteries and veins that are often connected by a fistula. The causes and mechanisms of these clinical entities are not fully understood. We discovered that suturing an arterial patch into the common jugular vein of rabbits led to spontaneous neovascularization, the formation of an arteriovenous fistula and the development of an arteriovenous shunt. An arterial patch excised from the common carotid artery was sutured into the common jugular vein. Within a month, a dense nidus-like neovasculature formed around the patch...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453346/anti-inflammatory-effect-of-tauroursodeoxycholic-acid-in-raw-264-7-macrophages-bone-marrow-derived-macrophages-bv2-microglial-cells-and-spinal-cord-injury
#10
Seong Jun Kim, Wan-Kyu Ko, Min-Jae Jo, Yoshie Arai, Hyemin Choi, Hemant Kumar, In-Bo Han, Seil Sohn
This study aimed to investigate the anti-inflammatory effects of tauroursodeoxycholic acid (TUDCA) after spinal cord injury (SCI) in rats. We induced an inflammatory process in RAW 264.7 macrophages, BV2 microglial cells, and bone marrow-derived macrophages (BMM) using lipopolysaccharide (LPS). The anti-inflammatory effects of TUDCA on LPS-stimulated RAW 264.7 macrophages, BV2 microglial cells, and BMMs were analyzed using nitric oxide (NO) assays, quantitative real-time polymerase chain reactions (qRT-PCR), and enzyme-linked immunosorbent assays (ELISA)...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453333/scleraxis-is-a-transcriptional-activator-that-regulates-the-expression-of-tenomodulin-a-marker-of-mature-tenocytes-and-ligamentocytes
#11
Chisa Shukunami, Aki Takimoto, Yuriko Nishizaki, Yuki Yoshimoto, Seima Tanaka, Shigenori Miura, Hitomi Watanabe, Tetsushi Sakuma, Takashi Yamamoto, Gen Kondoh, Yuji Hiraki
Tenomodulin (Tnmd) is a type II transmembrane glycoprotein predominantly expressed in tendons and ligaments. We found that scleraxis (Scx), a member of the Twist-family of basic helix-loop-helix transcription factors, is a transcriptional activator of Tnmd expression in tenocytes. During embryonic development, Scx expression preceded that of Tnmd. Tnmd expression was nearly absent in tendons and ligaments of Scx-deficient mice generated by transcription activator-like effector nucleases-mediated gene disruption...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453285/the-distal-upstream-region-of-insulin-like-growth-factor-binding-protein-1-enhances-its-expression-in-endometrial-stromal-cells-during-decidualization
#12
Isao Tamura, Kosuke Jozaki, Shun Sato, Yuichiro Shirafuta, Masahiro Shinagawa, Ryo Maekawa, Toshiaki Taketani, Hiromi Asada, Hiroshi Tamura, Norihiro Sugino
We have previously shown that decidualization of human endometrial stromal cells (ESCs) causes a genome-wide increase in the levels of acetylation of histone-H3 Lys-27 (H3K27ac). We also reported that the distal gene regions, more than 3 kb up or downstream of gene transcription start sites have increased H3K27ac levels. Insulin-like growth factor-binding protein-1 (IGFBP-1) is a specific decidualization marker and has increased H3K27ac levels in its distal upstream region (-4701 bp to -7501 bp). Here, using a luciferase reporter gene construct containing this IGFBP-1 upstream region, we tested the hypothesis that it is an IGFBP-1 enhancer...
February 16, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29453275/structure-based-discovery-of-selective-positive-allosteric-modulators-of-antagonists-for-the-m-2-muscarinic-acetylcholine-receptor
#13
Magdalena Korczynska, Mary J Clark, Celine Valant, Jun Xu, Ee Von Moo, Sabine Albold, Dahlia R Weiss, Hayarpi Torosyan, Weijiao Huang, Andrew C Kruse, Brent R Lyda, Lauren T May, Jo-Anne Baltos, Patrick M Sexton, Brian K Kobilka, Arthur Christopoulos, Brian K Shoichet, Roger K Sunahara
Subtype-selective antagonists for muscarinic acetylcholine receptors (mAChRs) have long been elusive, owing to the highly conserved orthosteric binding site. However, allosteric sites of these receptors are less conserved, motivating the search for allosteric ligands that modulate agonists or antagonists to confer subtype selectivity. Accordingly, a 4.6 million-molecule library was docked against the structure of the prototypical M 2 mAChR, seeking molecules that specifically stabilized antagonist binding. This led us to identify a positive allosteric modulator (PAM) that potentiated the antagonist N -methyl scopolamine (NMS)...
February 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29453044/micro-rna-1298-opposes-the-effects-of-chronic-oxidative-stress-on-human-trabecular-meshwork-cells-via-targeting-on-eif4e3
#14
Wu Ruibin, Xiaowei Zheng, Jiaying Chen, Xinyi Zhang, Xiayin Yang, Yuxian Lin
OBJECTIVE: This study aimed to investigate the effect and potential mechanism of miR-1298 in the progression of human trabecular meshwork (HTM) cells. MATERIAL AND METHODS: Expression of miR-1298 was assessed by quantitative real time PCR (qRT-PCR), as well as in HTM-1 and HTM-2 cells. Mature miR-1298 mimic, miR-1298 inhibitor, and si-EIF4E3 and their corresponding controls were transfected into HTM-1 and HTM-2 to obtain stable HTM cells. Luciferase reporter assay was used to verify regulation between miR-1298 and EIF4E3...
February 13, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29452367/variant-in-human-pofut1-reduces-enzymatic-activity-and-likely-causes-a-recessive-microcephaly-global-developmental-delay-with-cardiac-and-vascular-features
#15
Hideyuki Takeuchi, Derek Wong, Michael Schneider, Hudson H Freeze, Megumi Takeuchi, Steven J Berardinelli, Atsuko Ito, Hane Lee, Stanley F Nelson, Robert S Haltiwanger
Protein O-fucosyltransferase-1 (POFUT1) adds O-fucose monosaccharides to epidermal growth factor-like (EGF) repeats found on approximately 100 mammalian proteins, including Notch receptors. Haploinsufficiency of POFUT1 has been linked to adult-onset Dowling Degos Disease (DDD) with hyperpigmentation defects. Homozygous deletion of mouse Pofut1 results in embryonic lethality with severe Notch-like phenotypes including defects in somitogenesis, cardiogenesis, vasculogenesis, and neurogenesis, but the extent to which POFUT1 is required for normal human development is not yet understood...
February 14, 2018: Glycobiology
https://www.readbyqxmd.com/read/29452342/neurofilament-light-chain-and-oligoclonal-bands-are-prognostic-biomarkers-in-radiologically-isolated-syndrome
#16
Clara Matute-Blanch, Luisa M Villar, José C Álvarez-Cermeño, Konrad Rejdak, Evgeniy Evdoshenko, Gleb Makshakov, Vladimir Nazarov, Sergey Lapin, Luciana Midaglia, Angela Vidal-Jordana, Jelena Drulovic, Antonio García-Merino, Antonio J Sánchez-López, Eva Havrdova, Albert Saiz, Sara Llufriu, Roberto Alvarez-Lafuente, Ina Schroeder, Uwe K Zettl, Daniela Galimberti, Lluís Ramió-Torrentà, René Robles, Ester Quintana, Harald Hegen, Florian Deisenhammer, Jordi Río, Mar Tintoré, Alex Sánchez, Xavier Montalban, Manuel Comabella
The prognostic role of cerebrospinal fluid molecular biomarkers determined in early pathogenic stages of multiple sclerosis has yet to be defined. In the present study, we aimed to investigate the prognostic value of chitinase 3 like 1 (CHI3L1), neurofilament light chain, and oligoclonal bands for conversion to clinically isolated syndrome and to multiple sclerosis in 75 patients with radiologically isolated syndrome. Cerebrospinal fluid levels of CHI3L1 and neurofilament light chain were measured by enzyme-linked immunosorbent assay...
February 14, 2018: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29452292/prevalence-risk-factors-and-genetic-characterization-of-toxoplasma-gondii-in-sick-pigs-and-stray-cats-in-jiangsu-province-eastern-china
#17
Zhao-Feng Hou, Shi-Jie Su, Dan-Dan Liu, Le-le Wang, Chuan-Li Jia, Zhen-Xing Zhao, Yi-Fei Ma, Qiao-Qiao Li, Jin-Jun Xu, Jian-Ping Tao
Toxoplasma gondii is an obligate intracellular parasitic protozoan with a worldwide distribution. The parasites in edible tissues of pigs and oocysts from cats are the major sources of T. gondii infection in humans. However, there are no data from sick pigs in veterinary clinics or from stray cats in Jiangsu Province, eastern China. In total, biological samples from 141 sick pigs and 64 stray cats were collected from this region. The rate of T. gondii infection in sick pigs was 46.81% using a polymerase chain reaction (PCR), and the overall prevalence of toxoplasmosis in stray cats was 34...
February 13, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29452232/long-noncoding-rna-pvt1-promotes-emt-via-mediating-microrna-186-targeting-of-twist1-in-prostate-cancer
#18
Zhongwen Chang, Jun Cui, Yongsheng Song
The pathogenesis and the underlying mechanism of endothelial-mesenchymal transition in prostate cancer remain unclear. Plasmacytoma variant translocation 1 (PVT1), a novel long non-coding RNA maps to 8q24.21, and in many tumor studies the up-regulation of PVT1 has already been reported. PVT1 is closely related to tumor cell proliferation, invasion, and metastasis. In this study, we employed a combination of techniques to study the role of PVT1 in prostate cancer, which included bioinformatic analysis, Western blotting and cell migration assays of prostate cancer cell lines...
February 13, 2018: Gene
https://www.readbyqxmd.com/read/29452217/gene-expression-screening-identifies-cdca5-as-a-potential-therapeutic-target-in-acral-melanoma
#19
Tianxiao Xu, Meng Ma, Jie Dai, Sifan Yu, Xiaowen Wu, Huan Tang, Jiayi Yu, Junya Yan, Huan Yu, Zhihong Chi, Lu Si, Xinan Sheng, Chuanliang Cui, Yan Kong, Jun Guo
Acral melanoma (AM) is a rapidly progressing subtype of melanoma with poor prognosis. The complete array of molecular changes that occur during AM metastasis remains unclear. In this study, we compared the gene expression profiles of six primary and 12 lymph node metastatic AM samples by tissue microarray analysis. We found that the expression levels of 396 genes were increased, and that of 766 genes were decreased in the metastatic tissues compared with that in the primary tumors. The top 19 genes upregulated in the metastatic tissue specimens were selected for high-content short interfering (si)RNA screening...
February 13, 2018: Human Pathology
https://www.readbyqxmd.com/read/29451781/multiple-snps-detection-based-on-lateral-flow-assay-for-phenylketonuria-diagnostic
#20
Xiaonan Liu, Chao Zhang, Kewu Liu, Han Wang, Chaoxia Lu, Hang Li, Kai Hua, Juanli Zhu, Wenli Hui, Yali Cui, Xue Zhang
Single nucleotide polymorphisms (SNPs) are closely related to genetic diseases, but current SNP detection methods, such as DNA microarrays that include tedious procedures and expensive, sophisticated instruments, are unable to perform rapid SNPs detection in clinical practice, especially for those multiple SNPs related to genetic diseases. In this study, we report a sensitive, low cost, and easy-to-use point-of-care testing (POCT) system formed by combining amplification refractory mutation system (ARMS) polymerase chain reaction with gold magnetic nanoparticles (GMNPs) and lateral flow assay (LFA) noted as the ARMS-LFA system, which allow us to use a uniform condition for multiple SNPs detection simultaneously...
February 16, 2018: Analytical Chemistry
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