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https://www.readbyqxmd.com/read/28823121/characterization-of-the-porcine-nanog-5-flanking-region
#1
Azra Memon, Ki-Duk Song, Woon Kyu Lee
Objective: Nanog, a homeodomain protein, has been investigated in humans and mice using ESCs. Because of the limited availability of ESCs, few studies have reported the function and role of Nanog in porcine ESCs. Therefore, in this study, we investigated the location of the porcine Nanog chromosome and its basal promoter activity, which might have potential applications in development of ESs specific marker as well as understanding its operating systems in the porcine. Methods: To characterize the porcine Nanog promoter, the 5'-flanking region of Nanog was isolated from cells of mini-pig ears...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28822800/amelogenin-induces-m2-macrophage-polarisation-via-pge2-camp-signalling-pathway
#2
Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura
OBJECTIVES: Amelogenin, the major component of the enamel matrix derivative (EMD), has been suggested as a bioactive candidate for periodontal regeneration. Apart from producing a regenerative effect on periodontal tissues, amelogenin has also been reported to have an anti-inflammatory effect. However, the precise molecular mechanisms underlying these effects remain unclear. In the present study, we examined the immunomodulatory effects of amelogenin on macrophages. DESIGN: Human phorbol 12-myristate 13-acetate (PMA)-differentiated U937 macrophages and CD14+ peripheral blood-derived monocytes (PBMC)-derived macrophages were stimulated with recombinant amelogenin (rM180)...
August 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28822593/decreased-expression-of-microrna-122-is-associated-with-an-unfavorable-prognosis-in-childhood-acute-myeloid-leukemia-and-function-analysis-indicates-a-therapeutic-potential
#3
Juan Yang, Yufang Yuan, Xiaochun Yang, Ze Hong, Lijuan Yang
MicroRNA (miR)-122 functions as a tumor suppressor in various human cancers. However, its involvement in childhood acute myeloid leukemia (AML) remains unknown. In this study, quantitative real-time PCR assay demonstrated that miR-122 expression in bone marrow specimens from AML children were significantly lower than that in non-malignant controls (P<0.001). Statistically, AML children with low miR-122 expression more frequently had large white blood cell count (P=0.022), French-American-British classification subtype M7 (P<0...
June 28, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28822472/tens-like-stimulation-downregulates-inflammatory-cytokines-in-a-pc-12-cell-line
#4
Guy Sovak, Brian Budgell
OBJECTIVES: The purpose of this study was to evaluate the effects of transcutaneous electrical nerve stimulation (TENS)-like stimulation on the expression of the proinflammatory cytokines tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-6 in PC-12 cells, which are commonly used as neuronal cell models. METHODS: Nerve growth factor-differentiated PC-12 cells were exposed to electrical stimulation for 15 minutes at 1 mA, 200 μs, and 100 Hz. Cell lysate from stimulated and control cells was assayed for TNF-α, IL-1β, and IL-6...
July 2017: Journal of Manipulative and Physiological Therapeutics
https://www.readbyqxmd.com/read/28822306/xyloglucan-endotransglucosylase-hydrolases-xths-are-inactivated-by-binding-to-glass-and-cellulosic-surfaces-and-released-in-active-form-by-a-heat-stable-polymer-from-cauliflower-florets
#5
Sandra C Sharples, Tu C Nguyen-Phan, Stephen C Fry
Xyloglucan endotransglucosylase (XET) activity, which cuts and re-joins hemicellulose chains in the plant cell wall, contributing to wall assembly and growth regulation, is the major activity of XTH proteins. During purification, XTHs often lose XET activity which, however, is restored by treatment with certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets. It was not known whether the XTH-activating factor (XAF) present in CHPs works by promoting (e.g. allosterically) XET activity or by re-solubilising sequestered XTH proteins...
August 5, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28822174/-biological-assay-in-quality-control-of-animal-medicines
#6
REVIEW
Xuan Wang, Luo-Dan Ouyang, Chun-Mei Dai, Li Ma, Xiao-He Xiao
Animal medicine is a unique part of traditional Chinese medicine. They have strong effects, but their effective compounds are not entirely known. The efficiency and safety of animal medicines can't be effectively controlled by current quality assurance system and evaluation method, which has deeply influenced the development of animal medicines. Biological assay does not focus on efficacy of single component, but directly reflects the pharmacodynamics and safety of animal medicines by biological effect. With the development of biotechnology, many new technologies have emerged, such as biochip and high content analysis...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822077/increased-serum-levels-of-fetuin-b-in-patients-with-coronary-artery-disease
#7
Kefu Zhu, Yuming Wang, Pengqin Shu, Qinyi Zhou, Jinzhou Zhu, Wenjing Zhou, Changqing Du, Chenkai Xu, Xiaowei Liu, Lijiang Tang
BACKGROUND: Recent evidence indicates a pivotal role for fetuin B, one of the cystatin superfamily of cysteine protease inhibitors, in the pathogenesis of metabolic diseases. This study investigated whether serum fetuin B levels are associated with the presence of coronary artery disease. METHODS: Serum fetuin B levels were assessed in 87 patients with coronary artery disease (41 with acute coronary syndromes and 46 with stable angina pectoris) and 87 healthy controls using an enzyme-linked immunosorbent assay...
August 19, 2017: Endocrine
https://www.readbyqxmd.com/read/28821819/dietary-factors-and-polymorphisms-in-vitamin-d-metabolism-genes-the-risk-and-prognosis-of-colorectal-cancer-in-northeast-china
#8
Chen Gong, Zhiping Long, Yanming Yu, Lin Zhu, Jingshen Tian, Shuo Li, Jing Li, Hongyuan Yu, Qiang Chi, Daxun Piao, Fan Wang, Yashuang Zhao, Binbin Cui
CYP24A1 and CYP27B1 are critical genes determining 1α,25(OH)2D3 concentration and impacting on carcinogenesis. A case-control study including 528 colorectal cancer (CRC) patients and 605 cancer-free controls and a follow-up study with 317 cases were conducted in northeast China. Genotypes were tested by TaqMan Genotyping Assays. Individuals carrying the GG genotype of CYP27B1 G > T (rs10877012) exhibited decreased CRC risk compared with those with the TT genotype (ORadjusted (ORadj) = 0.57, 95% Confidence Interval (CI) = 0...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821793/molecular-mechanisms-of-atlastin-mediated-er-membrane-fusion-revealed-by-a-fret-based-single-vesicle-fusion-assay
#9
Kyung Tae Kim, Yeojin Moon, Yunsu Jang, Kang Taek Lee, Changwook Lee, Youngsoo Jun, Sanghwa Lee
Homotypic fusion of endoplasmic reticulum membranes is driven by atlastin GTPases; however, the underlying mechanism remains largely unknown. Here, using a FRET-based single-vesicle fusion assay with liposomes bearing the yeast atlastin Sey1p, we investigated the molecular mechanisms of atlastin-mediated membrane tethering and fusion. Although Sey1p-bearing proteoliposomes frequently underwent membrane tethering in a GTP hydrolysis-dependent manner as reported in studies using bulk assays, only a small fraction of the tethered liposomes proceeded to fusion...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821752/hybrid-nitric-oxide-donor-and-its-carrier-for-the-treatment-of-peripheral-arterial-diseases
#10
Duong Q Le, Aneetta E Kuriakose, Dat X Nguyen, Kytai T Nguyen, Suchismita Acharya
Nitric oxide (NO) has been known to promote physiological angiogenesis to treat peripheral arterial diseases (PAD) by increasing the vascular endothelial growth factor (VEGF) level in endothelial cells (ECs) and preventing platelet adherence and leukocyte chemotaxis. However, the ongoing ischemic event during peripheral ischemia produces superoxide and diminishes the NO bioavailability by forming toxic peroxynitrite anion. Here we disclose an efficacious hybrid molecule 4-(5-Amino-1,2,3-oxadiazol-3-yl)-2,2,6,6-tetramethyl-1-piperidinol (SA-2) containing both antioxidant and NO donor functionalities that provide a therapeutic level of NO necessary to promote angiogenesis and to protect ECs against hydrogen peroxide-induced oxidative stress...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821706/apigenin-ameliorates-post-stroke-cognitive-deficits-in-rats-through-histone-acetylation-mediated-neurochemical-alterations
#11
Fengxia Tu, Qiongyi Pang, Tingting Huang, Yun Zhao, Meixia Liu, Xiang Chen
BACKGROUND To identify the effect of apigenin on cognitive deficits of rats after cerebral ischemia and reperfusion injury, and to investigate the potential molecular mechanisms. MATERIAL AND METHODS The rats were given sodium butyrate (NaB) or apigenin (20 or 40 mg/kg) for 28 days. Cognition was investigated by the Morris water maze (MWM) test. On day 28, the rats were euthanized and their hippocampal brain regions were used to identify biochemical and neurochemical alterations. The content of histone deacetylase (HDAC) was measured by enzyme-linked immunosorbent assay (ELISA)...
August 19, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28821622/the-replication-and-transcription-activator-of-murine-gammaherpesvirus-68-cooperatively-enhances-cytokine-activated-stat3-mediated-gene-expression
#12
Hui-Chen Chang Foreman, Julie Armstrong, Alexis L Santana, Laurie T Krug, Nancy C Reich
Gammaherpesviruses (γΗVs) have a dynamic strategy for life-time persistence, involving productive infection, latency, and intermittent reactivation. In latency reservoirs, such as B lymphocytes, γΗV exists as a viral episome and expresses few viral genes. Although the ability of γHV to reactivate from latency and re-enter the lytic phase is challenging to investigate and control, it is known that the γΗV replication and transcription activator, RTA, can promote lytic reactivation. In this study, we provide first evidence that RTA of murine γΗV 68 (MHV68) selectively binds and enhances the activity of tyrosine-phosphorylated host STAT3...
August 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28821618/region-specific-protein-misfolding-cyclic-amplification-reproduces-brain-tropism-of-prion-strains
#13
Nicolas Privat, Etienne Levavasseur, Serfildan Yildirim, Samia Hannaoui, Jean-Philippe Brandel, Jean-Louis Laplanche, Vincent Béringue, Danielle Seilhean, Stéphane Haïk
Human prion diseases such as Creutzfeldt-Jakob disease are transmissible brain proteinopathies, characterized by the accumulation of a misfolded isoform of the host cellular prion protein (PrP) in the brain. According to the prion model, prions are defined as proteinaceous infectious particles composed solely of this abnormal isoform of PrP (PrPSc). Even in the absence of genetic material, various prion strains can be propagated in experimental models. They can be distinguished by the pattern of disease they produce and especially by the localization of PrPSc deposits within the brain and the spongiform lesions they induce...
August 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28821477/tgf-%C3%AE-induced-intracellular-pai-1-is-responsible-for-retaining-hematopoietic-stem-cells-in-the-niche
#14
Takashi Yahata, Abd Aziz Ibrahim, Yukari Muguruma, Mesut Eren, Alexander M Shaffer, Nobuo Watanabe, Satoko Kaneko, Tetsuo Nakabayashi, Takashi Dan, Noriaki Hirayama, Douglas E Vaughan, Toshio Miyata, Kiyoshi Ando
Hematopoietic stem and progenitor cells (HSPCs) reside in the supportive stromal niche in bone marrow (BM); when needed, however, they are rapidly mobilized into the circulation, suggesting that HSPCs are intrinsically highly motile but are usually stayed in the niche. We questioned what determines the motility of HSPCs. Here we show that transforming growth factor (TGF)-β-induced intracellular plasminogen activator inhibitor (PAI)-1 activation is responsible for keeping HSPCs in the BM niche. We found that the expression of PAI-1, a downstream target of TGF-β-signaling, was selectively augmented in niche-residing HSPCs...
August 18, 2017: Blood
https://www.readbyqxmd.com/read/28821258/contribution-of-serum-fgf21-level-to-the-identification-of-left-ventricular-systolic-dysfunction-and-cardiac-death
#15
Yun Shen, Xueli Zhang, Xiaoping Pan, Yiting Xu, Qin Xiong, Zhigang Lu, Xiaojing Ma, Yuqian Bao, Weiping Jia
AIM: The relationship between fibroblast growth factor 21 (FGF21) and cardiovascular disease has been well established in recent studies. This study aimed to investigate the relationship between FGF21 and left ventricular systolic dysfunction and cardiac death. METHODS: Two-dimensional echocardiography was used to measure the left ventricular ejection fraction (LVEF) to estimate left ventricular systolic function. The optimal cutoff of FGF21 for identifying left ventricular systolic dysfunction at baseline was analyzed via receiver operating characteristic (ROC) curves...
August 18, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28821234/incident-mycobacterium-tuberculosis-infection-in-household-contacts-of-infectious-tuberculosis-patients-in-brazil
#16
Edward C Jones-López, Carlos Acuña-Villaorduña, Geisa Fregona, Patricia Marques-Rodrigues, Laura F White, David Jamil Hadad, Lucilia Pereira Dutra-Molina, Solange Vinhas, Avery I McIntosh, Mary Gaeddert, Rodrigo Ribeiro-Rodrigues, Padmini Salgame, Moises Palaci, David Alland, Jerrold J Ellner, Reynaldo Dietze
BACKGROUND: In household contact investigations of tuberculosis (TB), a second tuberculin skin test (TST) obtained several weeks after a first negative result consistently identifies individuals that undergo TST conversion. It remains unclear whether this delay in M. tuberculosis infection is related to differences in the infectious exposure, TST boosting, partial host resistance, or some other factor. METHODS: We conducted a household contact study Vitória, Brazil...
August 18, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28821225/differences-in-survival-virulence-and-biofilm-formation-between-sialidase-deficient-and-w83-wild-type-porphyromonas-gingivalis-strains-under-stressful-environmental-conditions
#17
Xiaoyu Xu, Tong Tong, Xue Yang, Yaping Pan, Li Lin, Chen Li
BACKGROUND: Porphyromonas gingivalis is a major causative pathogen of chronic periodontitis. Within the inflammatory microenvironment, there exists extreme pH values, elevated temperatures and oxidative stress. Pathogens adapt to these stressful environmental conditions by regulating the transcription of virulence genes, modifying themselves with macromolecules and by aggregating and entering into a biofilm growth phase. Our previous study showed that the P. gingivalis sialidase can help cells obtain sialic acid from the environment, which is used to modify macromolecules on the surface of P...
August 18, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28821221/acute-penicillium-marneffei-infection-stimulates-host-m1-m2a-macrophages-polarization-in-balb-c-mice
#18
Xiaoying Dai, Congzheng Mao, Xiuwan Lan, Huan Chen, Meihua Li, Jing Bai, Jingmin Deng, Qiuli Liang, Jianquan Zhang, Xiaoning Zhong, Yi Liang, Jiangtao Fan, Honglin Luo, Zhiyi He
BACKGROUND: Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen that causes fatal infection. Alveolar macrophages are innate immune cells that have critical roles in protection against pulmonary fungal pathogens and the macrophage polarization state has the potential to be a deciding factor in disease progression or resolution. The aim of this study was to investigate mouse alveolar macrophage polarization states during P. marneffei infection. RESULTS: We used enzyme-linked immunosorbent (ELISA) assays, quantitative real-time PCR (qRT-PCR), and Griess, arginase activity to evaluate the phenotypic markers of alveolar macrophages from BALB/C mice infected with P...
August 18, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28821106/quantitative-assay-of-%C3%AE-1-3-%C3%AE-1-6-glucans-from-fermentation-broth-using-aniline-blue
#19
Steven Koenig, Broder Rühmann, Volker Sieber, Jochen Schmid
An aniline blue fluorescence assay was constructed to quantify two fungal β-(1,3)-glucans with β-(1,6)-sidechains: scleroglucan and schizophyllan. Calibration curves were linear in the range from 30mg/l to 6g/l with R(2)>99.8%. The assay was robust against d-glucose (50 g/l), oxalic acid (22.5g/l), protein (0.667g/l), and KCl (13.3g/l). Sclerotium rolfsii and Schizophyllum commune cultures were sampled, and the test results of the assay and alcohol precipitation were compared. The correlation between assay results of samples at different stages of the purification with the values from the precipitation of the polymers was the best in the supernatant for S...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821104/modulation-of-binding-to-vascular-endothelial-growth-factor-and-receptor-by-heparin-derived-oligosaccharide
#20
Jie-Ru Liu, Hui-Fang Wang, Dan-Feng Yu, Xiao-Yu Chen, Shu-Ying He
We investigated the mechanism of heparin-derived oligosaccharide on the proliferation of vascular smooth muscle cell (VSMC) induced by vascular endothelial growth factor (VEGF). Expression levels of VEGFR 1 and VEGFR 2 were examined by RT-PCR, and the corresponding protein expression levels were detected by Western blotting and immunocytochemistry. Western blotting was taken to identify the expression levels of mechanism proteins. The binding of VEGF and VEGFR 2 was measured by co-IP. Besides, HS competition assay was to detect the ability of HDO to compete with Heparin for VEGF165...
October 15, 2017: Carbohydrate Polymers
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