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https://www.readbyqxmd.com/read/28743116/the-role-of-tumoral-foxp3-on-cell-proliferation-migration-and-invasion-in-gastric-cancer
#1
Lu Zhang, Jianghao Xu, Xuan Zhang, Yi Zhang, Lu Wang, Xiaoxu Huang, Zekuan Xu
BACKGROUND/AIMS: There is little published data on the role of FOXP3 in gastric cancer. METHODS: FOXP3 expression and localization in gastric cancer tissues and cells were examined by immunohistochemistry, RT-PCR, flow cytometry, western blot, and laser confocal microscopy. CCK8, plate clone, wound healing, and transwell insert assays were performed for gastric cancer cells. Potential molecules and signaling pathways were screened using high-throughput transcriptome sequencing...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743103/lanthanum-chloride-inhibits-lps-mediated-expressions-of-pro-inflammatory-cytokines-and-adhesion-molecules-in-huvecs-involvement-of-nf-%C3%AE%C2%BAb-jmjd3-signaling
#2
Xia Chen, Min Xiu, Juanjuan Xing, Shaoqing Yu, Dinghong Min, Fei Guo
BACKGROUND/AIMS: To investigate the regulation of LaCl3 on lipopolysaccharides (LPS)-induced pro-inflammatory cytokines and adhesion molecules in human umbilical vein endothelial cells (HUVECs). METHODS: Primary cultured HUVECs were pretreated with 2.5 µM LaCl3 for 30 min followed by 1 µg/ml LPS for 2 h. Pro-inflammatory cytokine and adhesion molecule expressions were determined by real-time RT-PCR and ELISA. NF-κB/p65 nuclear translocation was examined by immunofluorescence and immuno-blot, and its DNA-binding activity was measured by chemiluminescence...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743072/molecular-modeling-and-molecular-dynamics-simulation-study-of-archaeal-leucyl-trna-synthetase-in-complex-with-different-mischarged-trna-in-editing-conformation
#3
A V Rayevsky, M Sharifi, M A Tukalo
Aminoacyl-tRNA synthetases (aaRSs) play important roles in maintaining the accuracy of protein synthesis. Some aaRSs accomplish this via editing mechanisms, among which leucyl-tRNA synthetase (LeuRS) edits non-cognate amino acid norvaline mainly by post-transfer editing. However, the molecular basis for this pathway for eukaryotic and archaeal LeuRS remain unclear. In this study, a complex of archaeal P. horikoshii LeuRS (PhLeuRS) with misacylated tRNA(Leu) was modeled wherever tRNA's acceptor stem was oriented directly into the editing site...
July 18, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28743036/recist-response-and-variation-of-circulating-tumour-cells-in-phase-1-trials-a-prospective-multicentric-study
#4
Christophe Massard, Isabelle Borget, Françoise Farace, Sandrine Aspeslagh, Marie-Cécile Le Deley, Christophe Le Tourneau, François-Clement Bidard, Jean-Yves Pierga, Veronique Dieras, Paul Hofman, Jean-Philippe Spano, Charles Ferte, Ludovic Lacroix, Jean-Charles Soria
BACKGROUND: Circulating tumour cell (CTC) counting could be a new biomarker for better evaluation of tumour response to molecules tested in phase I trials. PATIENTS AND METHODS: Consenting patients with advanced metastatic cancer referred to various phase I units were enrolled prospectively in this study. CTCs from 7.5 ml of whole blood drawn at baseline and after starting experimental therapy were counted using the CellSearch system, and tumour response was assessed using RECIST 1...
July 22, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28742986/adsorption-of-mesitylene-via-mesoporous-adsorbents
#5
Kai Chun Nien, Feng Tang Chang, Moo Been Chang
Mesitylene (or 1,3,5-trimethylbenzene) is a volatile organic compound emitted from various industrial processes, e.g., spray coating. Its emissions have become a critical issue because mesitylene is toxic and cannot be removed using traditional adsorbents, e.g., zeolite (H-ZSM-5; the diameter of mesitylene molecules is greater than the pore size of H-ZSM-5). Hence, an adsorbent with a large pore size, MCM-41, is used in this study to investigate its adsorption capacity for mesitylene and is compared with that of H-ZSM-5...
July 25, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28742966/the-decisive-role-of-hydrophobicity-on-the-effect-of-alkylammonium-chlorides-on-protein-stability-a-terahertz-spectroscopic-finding
#6
Debasish Das Mahanta, Rajib Kumar Mitra, Nirnay Samanta
Many biologically important processes involve a subtle interplay between Columbic and hydrophobic interactions among molecular groups with water. A comprehensive understanding of such processes, specially while occurring simultaneously in the same molecule is of practical importance. In this contribution we report the ultrafast (sub-ps to ps) collective hydrogen bond dynamics of water in the extended hydration layers in a series of alkylammonium chloride salts using THz time domain spectroscopic (TTDS) technique (0...
July 25, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28742960/tuning-the-redox-properties-of-fullerene-clusters-within-a-metal-organic-capsule
#7
Felix J Rizzuto, Daniel M Wood, Tanya K Ronson, Jonathan R Nitschke
A porphyrin-edged metal-organic tetrahedron was shown to form host-guest complexes containing 1-4 equivalents of fullerene C60, depending on the solvent employed. The molecules of C60 were bound in anti-cooperative fashion within well-defined pockets; an X ray crystal structure of three fullerenes inside the tetrahedron was obtained. Electrochemical measurements revealed that the electron-accepting properties of the fullerenes inside the capsules were altered depending on the mode of encapsulation. Encapsulation of a greater number of fullerenes was observed to increase the electron affinity of the overall cluster...
July 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28742822/metal-ion-coordination-in-the-e-coli-nudix-hydrolase-dihydroneopterin-triphosphate-pyrophosphatase-new-clues-into-catalytic-mechanism
#8
Shannon E Hill, Elaine Nguyen, Chiamaka U Ukachukwu, Dana M Freeman, Stephen Quirk, Raquel L Lieberman
Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because inhibition of enzymes in bacterial folate biosynthetic pathways is a strategy for antibiotic development. We determined crystal structures of DHNTPase with and without activating, Mg2+-mimicking metals Co2+ and Ni2+. Four metal ions, identified by anomalous scattering, and stoichiometrically confirmed in solution by isothermal titration calorimetry, are held in place by Glu56 and Glu60 within the Nudix sequence motif, Glu117, waters, and a sulfate ion, of which the latter is further stabilized by a salt bridge with Lys7...
2017: PloS One
https://www.readbyqxmd.com/read/28742538/anaphylaxis-and-intimate-behaviour
#9
Gennaro Liccardi, Marco Caminati, Gian Enrico Senna, Luigino Calzetta, Paola Rogliani
PURPOSE OF REVIEW: Intimate behaviours may represent an unusual way of exposure to a culprit allergen, or the frame for sex-related allergies due to triggers typically linked to that situation. The present review aims at summarizing the state of the art about the topic, in order to spread the awareness and the basic know-how in the field of sexual-related allergies. RECENT FINDINGS: Kiss-related IgE-mediated reactions are caused in sensitized partners mainly by the passive transport of allergenic molecules through saliva, skin or oral mucosa...
July 22, 2017: Current Opinion in Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28742490/observing-and-understanding-the-ultrafast-photochemistry-in-small-molecules-applications-to-sunscreens
#10
Lewis A Baker, Vasilios G Stavros
In this review, we discuss the importance of biological and artificial photoprotection against overexposure to harmful ultraviolet radiation. Transient electronic and transient vibrational absorption spectroscopies are highlighted as important tools in understanding the energy transfer in small molecules, with a focus on the application to commercial sunscreens with representative examples given. Oxybenzone, a common ingredient in commercial sunscreens and sinapoyl malate, a biological sunscreen in plant leaves are presented as case studies...
September 1, 2016: Science Progress
https://www.readbyqxmd.com/read/28742439/damab-2-anti-human-dgk%C3%AE-monoclonal-antibody-for-immunocytochemistry
#11
Tomoyuki Nakano, Satoshi Ogasawara, Toshiaki Tanaka, Yasukazu Hozumi, Satoru Mizuno, Eri Satoh, Fumio Sakane, Naoki Okada, Akinobu Taketomi, Ryusuke Honma, Takuro Nakamura, Noriko Saidoh, Miyuki Yanaka, Shunsuke Itai, Saori Handa, Yao-Wen Chang, Shinji Yamada, Mika K Kaneko, Yukinari Kato, Kaoru Goto
Diacylglycerol kinase (DGK) is responsible for the enzymatic conversion of diacylglycerol to phosphatidic acid. Since both diacylglycerol and phosphatidic acid serve as signaling molecules, DGK is regarded as a hub between diacylglycerol-mediated and phosphatidic acid-mediated signaling. One of the 10 DGK isozymes, DGKα, is shown to be involved in T cell function. Transfection studies using tagged expression vectors revealed that DGKα localizes to the cytoplasm and nucleus and translocates to the plasma membrane in response to T cell receptor stimulation...
July 25, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28742403/lpxc-inhibitors-a-patent-review-2010-2016
#12
Dmitrii V Kalinin, Ralph Holl
The Zn(2+)-dependent deacetylase LpxC is an essential enzyme of lipid A biosynthesis in Gram-negative bacteria and a promising target for the development of antibiotics selectively combating Gram-negative pathogens. Researchers from industry and academia have synthesized structurally diverse LpxC inhibitors, exhibiting different LpxC inhibitory and antibacterial activities. Areas covered: A brief introduction into the structure and function of LpxC, showing its suitability as antibacterial target, along with the structures of several reported LpxC inhibitors, is given...
July 25, 2017: Expert Opinion on Therapeutic Patents
https://www.readbyqxmd.com/read/28742368/borapetoside-e-a-clerodane-diterpenoid-extracted-from-tinospora-crispa-improves-hyperglycemia-and-hyperlipidemia-in-high-fat-diet-induced-type-2-diabetes-mice
#13
Yuhui Xu, Yanfen Niu, Yuan Gao, Fang Wang, Wanying Qin, Yanting Lu, Jing Hu, Li Peng, Jikai Liu, Wenyong Xiong
An insidious increase in the incidence of obesity, insulin resistance, and hyperlipidemia has led to an epidemic of type 2 diabetes worldwide. Tinospora crispa (T. crispa) is a familiar plant traditionally used in herbal medicine for diabetes; however, the major active ingredients of this plant are still unclear. In this study, we identified the therapeutic effects of borapetoside E, a small molecule extracted from T. crispa, in high-fat-diet (HFD)-induced obesity in mice. The therapeutic effects of borapetoside E in HFD-induced obese mice were assessed physiologically, histologically, and biochemically following intraperitoneal injection...
July 25, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28742364/solvent-exchange-leading-to-nanobubble-nucleation-a-molecular-dynamics-study
#14
Qianxiang Xiao, Yawei Liu, Zhenjiang Guo, Zhiping Liu, Detlef Lohse, Xianren Zhang
The solvent exchange procedure has become the most-used protocol to produce surface nanobubbles, while the molecular mechanisms behind the solvent exchange are far from being fully understood. In this paper, we build a simple model and use molecular dynam- ics simulations to investigate the dynamic characteristics of solvent exchange for producing nanobubbles. We find that at the first stage of solvent exchange, there exists an interface between interchanging solvents of different gas solubility. This interface moves towards the substrate gradually as the exchange process proceeds...
July 25, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28742353/a-dft-assessment-of-the-environment-polarity-effect-on-polyaniline-water-coupling
#15
Jasmina Nedkova Petrova, Anela N Ivanova, Alia V Tadjer
Crystallization water plays an important role for the self-organization of oligomer chains in conducting polyaniline - emeraldine. In order to quantify the interaction between the emeraldine salt and such a water, models containing a tetramer in bipolaronic or polaronic form, chloride counterions and an explicit water molecule are used. Different initial positions of water with respect to the oligomer chain - tangential and vertical, are considered. Various media are simulated by introducing implicit solvent continuum of decreasing polarity...
July 25, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28742348/an-observation-of-structural-wavepacket-motion-the-umbrella-mode-in-rydberg-excited-n-methyl-morpholine
#16
Yao Zhang, Sanghamitra Deb, Hannes Jonsson, Peter M M Weber
We have observed time-resolved, structural dynamics of a coherent vibrational wavepacket in Rydberg-excited N-methyl morpholine, a molecule with 48 internal degrees of freedom. The molecular structure was established by associating the time-dependent Rydberg electron binding energy, obtained from time-resolved photoionization-photoelectron spectroscopy, to the molecular structure using self-interaction corrected density functional calculations. Optical excitation at 226 nm launches an oscillatory wavepacket in the amine umbrella coordinate with a 650 fs period...
July 25, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28742347/cationic-and-anionic-impact-on-the-electronic-structure-of-liquid-water
#17
Zhong Yin, Ludger Inhester, Sreevidya Thekku Veedu, Wilson Quevedo, Annette Pietzsch, Philippe Wernet, Gerrit Groenhof, Alexander Foehlisch, Helmut Grubmüller, Simone Agnes Techert
Hydration shells around ions are crucial for many fundamental biological and chemical processes. Their local physico-chemical properties are quite different from bulk water, and hard to probe experimentally. We address this problem by combining soft X-ray spectroscopy using a liquid jet and molecular dynamics (MD) simulations together with ab initio electronic structure calculations to elucidate the water-ion interaction in an MgCl2 solution at the molecular level. Our results reveal that salt ions mainly affect the electronic properties of water molecules in close vicinity and that the oxygen K-edge X-ray emission spectrum of water molecules in the first solvation shell differs significantly from bulk water...
July 25, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28742344/insights-into-the-molybdenum-copper-heterometallic-cluster-assembly-in-the-orange-protein-probing-intermolecular-interactions-with-an-artificial-metal-binding-atcun-tag
#18
Biplab K Maiti, Rui M Almeida, Luisa B Maia, Isabel Moura, José J G Moura
Orange protein (ORP) is a small bacterial protein, of unknown function, that contains a unique molybdenum/copper heterometallic cluster, [S2Mo(VI)S2Cu(I)S2Mo(VI)S2](3-) (Mo/Cu), non-covalently bound. The native cluster can be reconstituted in a protein-assisted mode by the addition of Cu(II) plus tetrathiomolybdate to apo-ORP under controlled conditions. In the work described herein, we artificially inserted the ATCUN ("amino terminus Cu and Ni") motif in the Desulfovibrio gigas ORP (Ala1Ser2His3 followed by the native amino acid residues; modified protein abbreviated as ORP*) to increase our understanding of the Mo/Cu cluster assembly in ORP...
July 25, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28742339/vms-rot-a-new-module-of-the-virtual-multifrequency-spectrometer-for-simulation-interpretation-and-fitting-of-rotational-spectra
#19
Daniele Licari, Nicola Tasinato, Lorenzo Spada, Cristina Puzzarini, Vincenzo Barone
The Virtual Multifrequency Spectrometer (VMS) is a tool that aims at integrating a wide range of computational and experimental spectroscopic techniques with the final goal of disclosing the static and dynamic physical-chemical properties "hidden" in molecular spectra. VMS is composed of two parts, namely VMS-Comp, which provides access to the latest developments in the field of computational spectroscopy, and VMS-Draw, which provides a powerful graphical user interface (GUI) for an intuitive interpretation of theoretical outcomes and a direct comparison to experiment...
July 25, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28742227/melatonin-prevents-mitochondrial-dysfunctions-and-death-in-differentiated-skeletal-muscle-cells
#20
Sara Salucci, Michela Battistelli, Valentina Baldassarri, Debora Burini, Elisabetta Falcieri, Sabrina Burattini
Oxidative stress increase induces cellular damage and apoptosis activation, a mechanism believed to represent a final common pathway correlated to sarcopenia and many skeletal muscle disorders. The goal of this study is to evaluate if melatonin, a ROS scavenger molecule, is able to counteract or modulate myotube death. Here, differentiated C2C12 skeletal muscle cells have been treated with melatonin before chemicals known to induce apoptotic death and oxidative stress, and its effect has been investigated by means of morpho-functional analyses...
July 25, 2017: Microscopy Research and Technique
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