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amino acid metabolism

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https://www.readbyqxmd.com/read/29454090/characterization-of-vasa-homolog-in-a-neotropical-catfish-jundi%C3%A3-rhamdia-quelen-molecular-cloning-and-expression-analysis-during-embryonic-and-larval-development
#1
Juliana M B Ricci, Emanuel R M Martinez, Arno J Butzge, Lucas B Doretto, Marcos A Oliveira, Robie Allan Bombardelli, Jan Bogerd, Rafael H Nóbrega
We have characterized the full-length vasa cDNA from Jundiá, Rhamdia quelen (Heptapteridae, Siluriformes). vasa encodes a member of the DEAD-box protein family of ATP-dependent RNA helicases. This protein is highly conserved among different organisms and its role is associated with RNA metabolism. In the majority of the investigated species, vasa is restricted to the germ cell lineage and its expression has been used to study germline development in many organisms, including fish. The deduced R. quelen vasa amino acid sequence displayed high similarity with Vasa protein sequences from other organisms, and did not cluster with PL10 or P68 DEAD-box protein subfamilies...
February 14, 2018: Gene
https://www.readbyqxmd.com/read/29453970/proton-nmr-based-serum-metabolic-profile-correlates-with-the-neurological-recovery-in-treated-acute-spinal-cord-injury-asci-subjects-a-pilot-study
#2
Alka Singh, Rajeshwar Nath Srivastava, Ashok Agrahari, Suruchi Singh, Saloni Raj, Tanushri Chatterji, Abbas Ali Mahdi, Ravindra Kumar Garg, Raja Roy
BACKGROUND: Acute Spinal Cord Injury (ASCI) is still having substantial morbidity and mortality despite of advanced therapeutics. Major obstacles are paucity of monitoring tools or biomarkers for severity determination, recovery and prognostication. A prospective case control pilot study with serum 1 H NMR spectroscopic metabolic profiling was carried out to evaluate metabolites perturbations and its relationship with recovery and to see role of stem cells in facilitating neurological recovery...
February 14, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29453849/methionine-is-required-for-camp-pka-mediated-morphogenesis-and-virulence-of-candida-albicans
#3
Sanne Schrevens, Griet Van Zeebroeck, Michael Riedelberger, Hélène Tournu, Karl Kuchler, Patrick Van Dijck
Candida albicans is a major human fungal pathogen, causing superficial, as well as life-threatening invasive infections. Therefore, it has to adequately sense and respond to the host defense by expressing appropriate virulence attributes. The most important virulence factor of C. albicans is the yeast-to-hyphae morphogenetic switch, which can be induced by numerous environmental cues, including the amino acid methionine. Here, we show an essential role for methionine permease Mup1 in methionine-induced morphogenesis, biofilm formation, survival inside macrophages and virulence...
February 17, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29453600/argininosuccinate-synthetase-1-ass1-loss-in-high-grade-neuroendocrine-carcinomas-of-the-urinary-bladder-implications-for-targeted-therapy-with-adi-peg-20
#4
Sounak Gupta, Divya Sahu, John S Bomalaski, Igor Frank, Stephen A Boorjian, Prabin Thapa, John C Cheville, Donna E Hansel
High-grade neuroendocrine carcinomas (HGNECs) of the urinary bladder encompass small cell (SCNEC) and large cell neuroendocrine carcinomas (LCNEC). Currently, recommended initial management is with systemic chemotherapy, followed by consolidative therapy with either radical cystectomy or radiotherapy in patients with localized disease. Nevertheless, survival in this setting remains poor. We therefore evaluated the potential to modify arginine metabolism as an alternative, targeted therapy approach in these carcinomas...
February 16, 2018: Endocrine Pathology
https://www.readbyqxmd.com/read/29452407/dietary-l-glutamine-supplementation-improves-growth-performance-gut-morphology-and-serum-biochemical-indices-of-broiler-chickens-during-necrotic-enteritis-challenge
#5
G D Xue, R Barekatain, S B Wu, M Choct, R A Swick
Necrotic enteritis (NE) causes significant economic losses in the broiler chicken industry, especially in birds raised without in-feed antibiotics. L-glutamine (Gln) is an amino acid that may compensate for metabolic losses from infection and improve the intestinal development. This study investigated the effects of dietary Gln (10 g/kg) supplementation on growth performance, intestinal lesions, jejunum morphology, and serum biochemical indices of broiler chickens during NE challenge. The study employed a factorial arrangement of treatments with factors: NE challenge, no or yes; dietary Gln inclusion, 0 g/kg in starter (S), d 0 to 10, grower (G) d 10 to 24, and finisher (F) d 24 to 35; 10 g/kg in S, G, F, or 10 g/kg in S, G only...
February 14, 2018: Poultry Science
https://www.readbyqxmd.com/read/29450408/effect-of-arginase-1-inhibition-on-the-incidence-of-autoimmune-diabetes-in-nod-mice
#6
Luis F Hernandez, Peter Buchwald, Midhat H Abdulreda
Metabolism of the amino acid L-arginine is implicated in many physiological and pathophysiological processes including autoimmune conditions such as type 1 diabetes (T1D). Alternate arginine metabolism through the citrulline-nitric oxide (NO) or the ornithine pathways can lead to proinflammatory or immune regulatory effects, respectively. In this report, we blocked the arginine-ornithine metabolic pathway by inhibiting the enzyme arginase-1 with Nω-hydroxy-nor-arginine (nor-NOHA) to make arginine more available to the alternate citrulline pathway for augmented NO production and increased incidence of autoimmune T1D in female non-obese diabetic (NOD) mice...
January 2018: Current Research in Diabetes & Obesity Journal
https://www.readbyqxmd.com/read/29449867/current-therapies-in-alleviating-liver-disorders-and-cancers-with-a-special-focus-on-the-potential-of-vitamin-d
#7
REVIEW
Shahida Khan, Ashraf Ali, Sarah Khan, Ahmed Bakillah, Ghazi Damanhouri, Aziz Khan, Ahmed Makki, Ibtehal AlAnsari, Naheed Banu
Background: Liver dysfunction is a topic of global concern with many advancing therapies being researched. Though vitamin D takes a center place, other therapies especially nutritional are also gaining ground. Vitamin D has gone beyond its role in skeletal disorders by showcasing its associations in other metabolic dysfunctions too. Result: Epidemiological evidences show a correlation between the status of vitamin D and different forms of cancer. Vitamin D receptors and alterations in gene expression appear decisive in the development of chronic liver disorders...
2018: Nutrition & Metabolism
https://www.readbyqxmd.com/read/29447453/in-silico-genome-wide-identification-and-characterization-of-glutathione-s-transferase-gene-family-in-vigna-radiata-l-wilczek
#8
Swati Vaish, Praveen Awasthi, Siddharth Tiwari, Shailesh Kumar Tiwari, Divya Gupta, Mahesh Kumar Basantani
Plant glutathione S-transferases are integral to normal plant metabolism, and biotic and abiotic stress tolerance. GST gene family has been characterized in diverse plant species using molecular biology and bioinformatics approaches. In the current study, in silico analysis identified 44 GSTs in Vigna radiata. Of the total 44 GSTs identified, chromosomal locations of 31 GSTs were confirmed. The pI value of GST proteins ranged from 5.10 to 9.40. The predicted molecular weights ranged from 13.12 to 50 kDa. Subcellular localization analysis revealed that all GSTs were predominantly localized in the cytoplasm...
February 15, 2018: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/29447243/differences-in-the-intestinal-microbiota-between-uninfected-piglets-and-piglets-infected-with-porcine-epidemic-diarrhea-virus
#9
Mei-Zhou Huang, Sheng-Yi Wang, Hui Wang, Dong-An Cui, Ya-Jun Yang, Xi-Wang Liu, Xiao-Jun Kong, Jian-Yong Li
Porcine epidemic diarrhea, a disastrous gastrointestinal disease, causes great financial losses due to its high infectivity, morbidity and mortality in suckling piglets despite the development and application of various vaccines. In this study, high-throughput sequencing was used to explore differences in the intestinal microbiota between uninfected piglets and piglets infected with porcine epidemic diarrhea virus (PEDV). The results revealed that the small intestinal microbiota of suckling piglets infected with PEDV showed low diversity and was dominated by Proteobacteria (49...
2018: PloS One
https://www.readbyqxmd.com/read/29447216/genome-mining-of-streptomyces-scabrisporus-nf3-reveals-symbiotic-features-including-genes-related-to-plant-interactions
#10
Corina Diana Ceapă, Melissa Vázquez-Hernández, Stefany Daniela Rodríguez-Luna, Angélica Patricia Cruz Vázquez, Verónica Jiménez Suárez, Romina Rodríguez-Sanoja, Elena R Alvarez-Buylla, Sergio Sánchez
Endophytic bacteria are wide-spread and associated with plant physiological benefits, yet their genomes and secondary metabolites remain largely unidentified. In this study, we explored the genome of the endophyte Streptomyces scabrisporus NF3 for discovery of potential novel molecules as well as genes and metabolites involved in host interactions. The complete genomes of seven Streptomyces and three other more distantly related bacteria were used to define the functional landscape of this unique microbe. The S...
2018: PloS One
https://www.readbyqxmd.com/read/29445454/rumen-protected-methionine-during-the-peripartal-period-in-dairy-cows-and-its-effects-on-abundance-of-major-species-of-ruminal-bacteria
#11
Mohamed K Abdelmegeid, Ahmed A Elolimy, Zheng Zhou, Vincenzo Lopreiato, Joshua C McCann, Juan J Loor
Background: Extensive degradation of amino acids in the rumen via microbial deamination decreases the post-ruminal availability of dietary indispensable amino acids. Together with the normal decrease in voluntary dry matter intake (DMI) around parturition in dairy cows, microbial metabolism contributes to a markedly negative balance of indispensable amino acids, including methionine which may be the first-limiting for milk production. The main objective of the current study was to profile changes in major bacterial species with key functions in cellulose and hemicellulose digestion, xylan breakdown, proteolytic action, propionic acid production, lactate utilization and ruminal biohydrogenation in cows supplemented with rumen-protected Methionine (SM; Smartamine M, Adisseo NA, Alpharetta, GA, USA) from -23 through 30 d relative to parturition...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29445145/canagliflozin-mediated-dual-inhibition-of-mitochondrial-glutamate-dehydrogenase-and-complex-i-an-off-target-adverse-effect
#12
Philipp F Secker, Sascha Beneke, Nadja Schlichenmaier, Johannes Delp, Simon Gutbier, Marcel Leist, Daniel R Dietrich
Recent FDA Drug Safety Communications report an increased risk for acute kidney injury in patients treated with the gliflozin class of sodium/glucose co-transport inhibitors indicated for treatment of type 2 diabetes mellitus. To identify a potential rationale for the latter, we used an in vitro human renal proximal tubule epithelial cell model system (RPTEC/TERT1), physiologically representing human renal proximal tubule function. A targeted metabolomics approach, contrasting gliflozins to inhibitors of central carbon metabolism and mitochondrial function, revealed a double mode of action for canagliflozin, but not for its analogs dapagliflozin and empagliflozin...
February 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29444855/role-of-glutathione-metabolism-in-host-defense-against-borrelia-burgdorferi-infection
#13
Mariska Kerstholt, Hedwig Vrijmoeth, Ekta Lachmandas, Marije Oosting, Mihaela Lupse, Mirela Flonta, Charles A Dinarello, Mihai G Netea, Leo A B Joosten
Pathogen-induced changes in host cell metabolism are known to be important for the immune response. In this study, we investigated how infection with the Lyme disease-causing bacterium Borrelia burgdorferi ( Bb ) affects host metabolic pathways and how these metabolic pathways may impact host defense. First, metabolome analysis was performed on human primary monocytes from healthy volunteers, stimulated for 24 h with Bb at low multiplicity of infection (MOI). Pathway analysis indicated that glutathione (GSH) metabolism was the pathway most significantly affected by Bb Specifically, intracellular levels of GSH increased on average 10-fold in response to Bb exposure...
February 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29444636/transcriptome-analysis-of-different-growth-stages-of-aspergillus-oryzae-reveals-dynamic-changes-of-distinct-classes-of-genes-during-growth
#14
Bin He, Zhihong Hu, Long Ma, Haoran Li, Mingqiang Ai, Jizhong Han, Bin Zeng
BACKGROUND: The gene expression profile and metabolic pathways of Aspergillus oryzae underlying the anatomical and morphological differentiation across different growth stages have not been fully characterized. The rapid development of next-generation sequencing technologies provides advanced knowledge of the genomic organization of A. oryzae. RESULTS: In this study, we characterized the growth and development of A. oryzae at different growth stages, including the adaptive phase, logarithmic phase, and stationary phase...
February 14, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29444218/genomic-and-physiological-characterization-and-description-of-marinobacter-gelidimuriae-sp-nov-a-psychrophilic-moderate-halophile-from-blood-falls-an-antarctic-subglacial-brine
#15
Michelle J Chua, Richard L Campen, Lindsay Wahl, Joseph J Grzymski, Jill A Mikucki
Antarctic subice environments are diverse, underexplored microbial habitats. Here we describe the ecophysiology and annotated genome of a Marinobacter strain isolated from a cold, saline, iron-rich subglacial outflow of the Taylor Glacier, Antarctica. This strain (BF04_CF4) grows fastest at neutral pH (range 6-10), is psychrophilic (range: 0-20°C), moderately halophilic (range: 0.8-15% NaCl), and hosts genes encoding potential low temperature and high salt adaptations. The predicted proteome suggests it utilizes fewer charged amino acids than a mesophilic Marinobacter strain...
February 10, 2018: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29444171/reduced-cognitive-function-increased-blood-brain-barrier-transport-and-inflammatory-responses-and-altered-brain-metabolites-in-ldlr-and-c57bl-6-mice-fed-a-western-diet
#16
Jennifer M Rutkowsky, Linda L Lee, Michelle Puchowicz, Mari S Golub, Douglas E Befroy, Dennis W Wilson, Steven Anderson, Gary Cline, Jason Bini, Kamil Borkowski, Trina A Knotts, John C Rutledge
Recent work suggests that diet affects brain metabolism thereby impacting cognitive function. Our objective was to determine if a western diet altered brain metabolism, increased blood-brain barrier (BBB) transport and inflammation, and induced cognitive impairment in C57BL/6 (WT) mice and low-density lipoprotein receptor null (LDLr -/-) mice, a model of hyperlipidemia and cognitive decline. We show that a western diet and LDLr -/- moderately influence cognitive processes as assessed by Y-maze and radial arm water maze...
2018: PloS One
https://www.readbyqxmd.com/read/29444145/waggawagga-cli-a-command-line-tool-for-predicting-stable-single-%C3%AE-helices-sah-domains-and-the-sah-domain-distribution-across-eukaryotes
#17
Dominic Simm, Martin Kollmar
Stable single-alpha helices (SAH-domains) function as rigid connectors and constant force springs between structural domains, and can provide contact surfaces for protein-protein and protein-RNA interactions. SAH-domains mainly consist of charged amino acids and are monomeric and stable in polar solutions, characteristics which distinguish them from coiled-coil domains and intrinsically disordered regions. Although the number of reported SAH-domains is steadily increasing, genome-wide analyses of SAH-domains in eukaryotic genomes are still missing...
2018: PloS One
https://www.readbyqxmd.com/read/29443975/features-and-regulation-of-non-enzymatic-post-translational-modifications
#18
REVIEW
Robert Harmel, Dorothea Fiedler
Non-enzymatic post-translational modifications of proteins can occur when a nucleophilic or redox-sensitive amino acid side chain encounters a reactive metabolite. In many cases, the biological function of these modifications is limited by their irreversibility, and consequently these non-enzymatic modifications are often considered as indicators of stress and disease. Certain non-enzymatic post-translational modifications, however, can be reversed, which provides an additional layer of regulation and renders these modifications suitable for controlling a diverse set of cellular processes ranging from signaling to metabolism...
February 14, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29442725/effect-of-bulk-mos%C3%A2-on-the-metabolic-profile-of-yeast
#19
Yadong Yu, Qi Yang, Na Wu, Hanlin Tang, Yanliang Yi, Gaihong Wang, Yilin Ge, Jiajun Zong, Catherine Madzak, Ye Zhao, Ling Jiang, He Huang
MoS2, a kind of two-dimensional material with unique performances, has been widely used in many fields. However, an in-depth understanding of its toxicity is still needed, let alone its effects on the environmental microorganism. Herein, we used different methods, including metabolomics technology, to investigate the influence of bulk MoS2 (BMS) on yeast cells. The results indicated that high concentrations (1 mg/L and more) of BMS could destroy cell membrane and induce ROS accumulation. When exposed to a low concentration of BMS (0...
June 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442259/lc-ms-based-serum-metabolomics-reveals-a-distinctive-signature-in-patients-with-rheumatoid-arthritis
#20
Ju Li, Nan Che, Lingxiao Xu, Qian Zhang, Qi Wang, Wenfeng Tan, Miaojia Zhang
Metabolomics has been applied to explore altered metabolite profiles in disease and identify unique metabolic signatures in recent years. We aim to characterize the metabolic profile of rheumatoid arthritis patients and explore its underlying pathological processes using metabolomics approach. Serum samples from 30 rheumatoid arthritis (RA) patients, 30 primary Sjogren's syndrome (pSS) patients, and 32 healthy controls (HC) were collected. The sample was analyzed by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS)...
February 13, 2018: Clinical Rheumatology
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