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https://www.readbyqxmd.com/read/28448627/engineering-chimeric-human-and-mouse-major-histocompatibility-complex-mhc-class-i-tetramers-for-the-production-of-t-cell-receptor-tcr-mimic-antibodies
#1
Demin Li, Carol Bentley, Jenna Yates, Maryam Salimi, Jenny Greig, Sarah Wiblin, Tasneem Hassanali, Alison H Banham
Therapeutic monoclonal antibodies targeting cell surface or secreted antigens are among the most effective classes of novel immunotherapies. However, the majority of human proteins and established cancer biomarkers are intracellular. Peptides derived from these intracellular proteins are presented on the cell surface by major histocompatibility complex class I (MHC-I) and can be targeted by a novel class of T-cell receptor mimic (TCRm) antibodies that recognise similar epitopes to T-cell receptors. Humoural immune responses to MHC-I tetramers rarely generate TCRm antibodies and many antibodies recognise the α3 domain of MHC-I and β2 microglobulin (β2m) that are not directly involved in presenting the target peptide...
2017: PloS One
https://www.readbyqxmd.com/read/28448603/evaluation-of-immunogenicity-and-protective-efficacy-of-recombinant-outer-membrane-proteins-of-haemophilus-parasuis-serovar-5-in-a-murine-model
#2
Miao Li, Ru-Jian Cai, Shuai Song, Zhi-Yong Jiang, Yan Li, Hong-Chao Gou, Pin-Pin Chu, Chun-Ling Li, Hua-Ji Qiu
Glässer's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28448594/novel-in-natural-infection-subdominant-hiv-1-cd8-t-cell-epitopes-revealed-in-human-recipients-of-conserved-region-t-cell-vaccines
#3
Nicola Borthwick, Zhansong Lin, Tomohiro Akahoshi, Anuska Llano, Sandra Silva-Arrieta, Tina Ahmed, Lucy Dorrell, Christian Brander, Hayato Murakoshi, Masafumi Takiguchi, Tomáš Hanke
BACKGROUND: Fine definition of targeted CD8+ T-cell epitopes and their human leucocyte antigen (HLA) class I restriction informs iterative improvements of HIV-1 T-cell vaccine designs and may predict early vaccine success or failure. Here, lymphocytes from volunteers, who had received candidate HIVconsv vaccines expressing conserved sub-protein regions of HIV-1, were used to define the optimum-length target epitopes and their HLA restriction. In HIV-1-positive patients, CD8+ T-cell responses predominantly recognize immunodominant, but hypervariable and therefore less protective epitopes...
2017: PloS One
https://www.readbyqxmd.com/read/28448521/immunization-with-toxoplasma-gondii-peroxiredoxin-1-induces-protective-immunity-against-toxoplasmosis-in-mice
#4
Ragab M Fereig, Yasuhiro Kuroda, Mohamad Alaa Terkawi, Motamed Elsayed Mahmoud, Yoshifumi Nishikawa
To develop a vaccine against Toxoplasma gondii, a vaccine antigen with immune-stimulating activity is required. In the present study, we investigated the immunogenicity and prophylactic potential of T. gondii peroxiredoxin 1 (TgPrx1). The TgPrx1 was detected in the ascitic fluid of mice 6 days postinfection, while specific antibody levels were low in the sera of chronically infected mice. Treatment of murine peritoneal macrophages with recombinant TgPrx1 triggered IL-12p40 and IL-6 production, but not IL-10 production...
2017: PloS One
https://www.readbyqxmd.com/read/28446665/dense-array-of-spikes-on-hiv-1-virion-particles
#5
Armando Stano, Daniel P Leaman, Arthur S Kim, Lei Zhang, Ludovic Autin, Jidnyasa Ingale, Syna K Gift, Jared Truong, Richard T Wyatt, Arthur J Olson, Michael B Zwick
HIV-1 is rare among viruses for having a low number of envelope glycoprotein (Env) spikes per virion, i.e. ∼7-14. This exceptional feature has been associated with avoidance of humoral immunity, i.e. B cell activation and antibody neutralization. Virus-like particles (VLPs) with increased density of Env are being pursued for vaccine development; however these typically require protein engineering that alters Env structure. Here, we used instead a strategy that targets the producer cell. We employed fluorescence activated cell sorting (FACS) to sort for cells that are recognized by trimer crossreactive broadly neutralizing antibody (bnAb) and not by non-neutralizing antibodies...
April 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28446441/long-term-persistence-of-cell-mediated-and-humoral-responses-to-a-h1n1-pdm09-influenza-virus-vaccines-and-the-role-of-the-as03-adjuvant-system-in-adults-during-two-randomized-controlled-trials
#6
Robbert G van der Most, Frédéric Clément, Julie Willekens, Walthère Dewé, Karl Walravens, David W Vaughn, Geert Leroux-Roels
We investigated the role of AS03, an α-tocopherol oil-in-water-emulsion-based Adjuvant System, on the long-term persistence of humoral and cell-mediated immune responses to A(H1N1)pdm09 influenza vaccines. In two studies (NCT00968539/NCT00989287), a total of 261 healthy adults (age ≤60 years) were randomized to receive two doses of AS03-adjuvanted vaccine containing 3.75 μg hemagglutinin, or non-adjuvanted vaccine containing 15 μg hemagglutinin (in Study A) or 3.75 μg hemagglutinin (in Study B), 21 days apart...
April 26, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28446245/mining-a-differential-sialotranscriptome-of-rhipicephalus-microplus-guides-antigen-discovery-to-formulate-a-vaccine-that-reduces-tick-infestations
#7
Sandra R Maruyama, Gustavo R Garcia, Felipe R Teixeira, Lucinda G Brandão, Jennifer M Anderson, José M C Ribeiro, Jesus G Valenzuela, Jana Horackova, Cecília J Veríssimo, Luciana M Katiki, Tamy M Banin, Amanda F Zangirolamo, Luiz G Gardinassi, Beatriz R Ferreira, Isabel K F de Miranda-Santos
BACKGROUND: Ticks cause massive damage to livestock and vaccines are one sustainable substitute for the acaricides currently heavily used to control infestations. To guide antigen discovery for a vaccine that targets the gamut of parasitic strategies mediated by tick saliva and enables immunological memory, we exploited a transcriptome constructed from salivary glands from all stages of Rhipicephalus microplus ticks feeding on genetically tick-resistant and susceptible bovines. RESULTS: Different levels of host anti-tick immunity affected gene expression in tick salivary glands; we thus selected four proteins encoded by genes weakly expressed in ticks attempting to feed on resistant hosts or otherwise abundantly expressed in ticks fed on susceptible hosts; these sialoproteins mediate four functions of parasitism deployed by male ticks and that do not induce antibodies in naturally infected, susceptible bovines...
April 26, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28446190/recognition-of-plasmodium-falciparum-mature-gametocyte-infected-erythrocytes-by-antibodies-of-semi-immune-adults-and-malaria-exposed-children-from-gabon
#8
Tamirat Gebru, Anthony Ajua, Michael Theisen, Meral Esen, Ulysse Ateba Ngoa, Saadou Issifou, Ayola A Adegnika, Peter G Kremsner, Benjamin Mordmüller, Jana Held
BACKGROUND: Transmission of malaria from man to mosquito depends on the presence of gametocytes, the sexual stage of Plasmodium parasites in the infected host. Naturally acquired antibodies against gametocytes exist and may play a role in controlling transmission by limiting the gametocyte development in the circulation or by interrupting gamete development and fertilization in the mosquito following ingestion. So far, most studies on antibody responses to sexual stage antigens have focused on a subset of gametocyte-surface antigens, even though inhibitory Ab responses to other gametocyte antigens might also play a role in controlling gametocyte density and fertility...
April 26, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28446061/signaling-by-antibodies-recent-progress
#9
Stylianos Bournazos, Taia T Wang, Rony Dahan, Jad Maamary, Jeffrey V Ravetch
IgG antibodies mediate a diversity of immune functions by coupling of antigen specificity through the Fab domain to signal transduction via Fc-Fc receptor interactions. Indeed, balanced IgG signaling through type I and type II Fc receptors is required for the control of proinflammatory, anti-inflammatory, and immunomodulatory processes. In this review, we discuss the mechanisms that govern IgG-Fc receptor interactions, highlighting the diversity of Fc receptor-mediated effector functions that regulate immunity and inflammation as well as determine susceptibility to infection and autoimmunity and responsiveness to antibody-based therapeutics and vaccines...
April 26, 2017: Annual Review of Immunology
https://www.readbyqxmd.com/read/28445973/plasmodium-parasite-as-an-effective-hepatocellular-carcinoma-antigen-glypican-3-delivery-vector
#10
Quan Liu, Yijun Yang, Xuefang Tan, Zhu Tao, Dickson Adah, Songlin Yu, Junnan Lu, Siting Zhao, Limei Qin, Li Qin, Xiaoping Chen
We have previously demonstrated that malaria parasite infection has an anti-tumor effect in a mouse model. This research aimed to investigate the possibility of using Plasmodium parasite as a novel vaccine vector for hepatocellular carcinoma (HCC) immunotherapy. We constructed a Plasmodium yoelii 17XNL strain (P.y) expressing murine glypican-3 (GPC3) protein (P.y-GPC3), and examined its therapeutic potency in a murine Hepa1-6-induced hepatoma model that highly expressed GPC3 protein. The prerequisites for invoking a CD8+ T cell response were assessed after P...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445927/vaccines-targeting-pres1-domain-overcome-immune-tolerance-in-hbv-carrier-mice
#11
Yingjie Bian, Zheng Zhang, Zhichen Sun, Juanjuan Zhao, Danming Zhu, Yang Wang, Sherry Fu, Jingya Guo, Longchao Liu, Lishan Su, Fu-Sheng Wang, Yang-Xin Fu, Hua Peng
Strong tolerance to HBV surface antigens limits the therapeutic effect of the conventional HBsAg vaccination in both preclinical animal models and patients with chronic hepatitis B (CHB) infection. In contrast, we observed that clinical CHB patients presented less immune tolerance to the preS1 domain of HBV large surface antigen. To study whether targeting the weak tolerance of preS1 region could improve therapy gain, we explored vaccination with the long peptide of preS1 domain for HBV virions clearance. Our study showed that this preS1-polypeptide rather than HBsAg vaccination induced robust immune responses in the HBV carrier mice...
April 26, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28445856/procyanidin-a-kind-of-biological-flavonoid-induces-protective-anti-tumor-immunity-and-protects-mice-from-lethal-b16f10-challenge
#12
Lina Zhang, Shuang Wang, Zeyuan Liu, Li Zhang, Shanzheng Wang, Bin Wang
Recently, increasing evidences show that procyanidin (PC) modulate immune responses in human. To evaluate adjuvant effects of PC on vaccine immune modulation and anti-tumor activity, we formulated PC with B16F10 tumor antigen as tumor vaccine to immune C57BL/6 mice and used intramuscular injection before challenge with tumor B16F10 cells. Our results revealed that PC enhanced T cell-mediated immune responses both in vitro and in vivo. Moreover, the B16F10 tumor vaccine induced some degree of anti-tumor effects as evaluated by the inhibition of tumor growth and the prolongation of survival...
April 23, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28445638/antigenic-gm3-lactone-mimetic-molecule-integrated-mannosylated-glycopeptide-nanofibers-for-the-activation-and-maturation-of-dendritic-cells
#13
Gokhan Gunay, Melis Sardan Ekiz, Xhenti Ferhati, Barbara Richichi, Cristina Nativi, Ayse Begum Tekinay, Mustafa O Guler
The ability of dendritic cells to coordinate innate and adaptive immune responses makes them essential targets for vaccination strategies. Presentation of specific antigens by dendritic cells is required for the activation of the immune system against many pathogens, and nanoscale materials can be functionalized for active targeting of dendritic cells. In this work, we integrated an immunogenic, carbohydrate melanoma-associated antigen-mimetic GM3-lactone molecule into mannosylated peptide amphiphile nanofibers to target dendritic cells through DC-SIGN receptor...
April 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28445612/influence-of-adjuvant-formulation-on-inducing-immune-response-in-mice-immunized-with-a-recombinant-serpin-from-trichinella-spiralis
#14
Jing Xu, Xue Bai, Libo Wang, Hai Ning Shi, Joke W B van der Giessen, Pascal Boireau, Ming Yuan Liu, Xiao Lei Liu
Nematodes of the genus Trichinella are one of the most widespread zoonotic pathogens on the world and they can still cause major public health problems in many parts of the world. Vaccination against the helminth nematode Trichinella could be a good strategy to reduce the risk of human and animal infection. It was our aim to evaluate three adjuvants, which could be used as an efficient vaccine for animals in combination with rTs-Serpin antigen. In this study, BALB/c mice were vaccinated by an intramuscular route with rTs-Serpin antigen from the parasite Trichinella spiralis in combination with three different adjuvant formulations: Montanide ISA201, Montanide IMS 1313 N PR VG and Freund's complete adjuvant / Freund's incomplete adjuvant (FCA/FIA)...
April 26, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28444127/hla-class-i-binding-prediction-via-convolutional-neural-networks
#15
Yeeleng S Vang, Xiaohui Xie
Motivation: Many biological processes are governed by protein-ligand interactions. One such example is the recognition of self and nonself cells by the immune system. This immune response process is regulated by the major histocompatibility complex (MHC) protein which is encoded by the human leukocyte antigen (HLA) complex. Understanding the binding potential between MHC and peptides can lead to the design of more potent, peptide-based vaccines and immunotherapies for infectious autoimmune diseases...
April 21, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28441089/one-size-fits-all-antibody-avidity-measurement-against-multiple-antigens-in-maternal-vaccination-studies
#16
Thomas Rice, Beate Kampmann, Beth Holder
No abstract text is available yet for this article.
April 25, 2017: Virulence
https://www.readbyqxmd.com/read/28440707/recent-developments-in-nanocarrier-aided-mucosal-vaccination
#17
Olga Kammona, Vassilis Bourganis, Theodora Karamanidou, Costas Kiparissides
To date, most of the licensed vaccines for mucosal delivery are based on live-attenuated viruses which carry the risk of regaining their pathogenicity. Therefore, the development of efficient nonviral vectors allowing the induction of potent humoral and cell-mediated immunity is regarded as an imperative scientific challenge as well as a commercial breakthrough for the pharma industries. For a successful translation to the clinic, such nanocarriers should protect the antigens from mucosal enzymes, facilitate antigen uptake by microfold cells and allow the copresentation of robust, safe for human use, mucosal adjuvants to antigen-presenting cells...
April 25, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28440596/immunogenicity-of-a-fully-synthetic-muc1-glycopeptide-antitumor-vaccine-enhanced-by-poly-i-c-as-tlr3-activating-adjuvant
#18
Horst Kunz, Markus Glaffig, Natascha Stergiou, Edgar Schmitt
Fully synthetic MUC1 glycopeptide antitumor vaccines have an exactly specified structure, and induce a targeted immune response without suppression of the immune response when using an immunogenic carrier protein. However, tumor-associated aberrantly glycosylated MUC1 glycopeptides are endogenous structures, "self-antigens", which exhibit only low immunogenicity. To overcome this obstacle, a fully synthetic MUC1 glycopeptide antitumor vaccine 1 was combined with polyinosinic:polycytidylic acid, poly(I:C), as a structurally defined Toll-like receptor 3 activating adjuvant...
April 25, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28440187/genetic-modifications-of-icosahedral-plant-virus-based-nanoparticles-for-vaccine-and-immunotherapy-applications
#19
Kannan Badri Narayanan, Sung Soo Han
Vaccine development is one of the greatest achievements of modern medicine. Vaccines made of live-attenuated pathogens can revert to virulent live strains, which causes safety concerns. On the other hand, the use of purified antigenic components as subunit vaccines is safer, but less effective, as these components induce lower levels of protective immunity. Multiple copy presentation of an antigenic determinant in a well-ordered and well-defined orientation on a nanosized particle can mimic the natural host-pathogen surface interaction to provide antigen stability and immunogenicity similar to that of conventional vaccines with improved safety...
April 24, 2017: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/28439708/hepatitis-b-virus-infection-assessed-3-to-18%C3%A2-years-after-vaccination-in-cuban-children-and-adolescents-born-to-hbsag-positive-mothers
#20
Licel de Los Angeles Rodríguez Lay, Marité Bello Corredor, Maria Caridad Montalvo Villalba, Annia Gertrudis Chibás Ojeda, Susel Sariego Frómeta, Manuel Diaz González, Yoandra Abad Lamoth, Meilin Sánchez Wong, Aurélie Sausy, Claude P Muller, Judith M Hübschen
Thirty-two participants, aged between 3-18 years, born to hepatitis B surface antigen (HBsAg)-positive mothers and vaccinated at birth were analyzed for hepatitis B virus (HBV) infection. Overall, 56% had anti-HB titers ≥10 IU/L; five were positive for antibodies to the core antigen (anti-HBc), and two of these were also positive for HBsAg/DNA. One of the HBsAg/anti-HBc double-negative children presented with an unusual occult infection (HBV DNA-positive). No known vaccine escape mutations were detectable...
April 24, 2017: Archives of Virology
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