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https://www.readbyqxmd.com/read/28439137/compatibility-of-ceftazidime-avibactam-ceftolozane-tazobactam-and-piperacillin-tazobactam-with-vancomycin-in-dextrose-5-in-water
#1
Kevin Meyer, Maressa Santarossa, Larry H Danziger, Eric Wenzler
Objectives: The compatibility of vancomycin with existing and novel β-lactam/β-lactamase inhibitors at clinically relevant concentrations in 5% dextrose in water has not been fully explored to date. Methods: Vancomycin concentrations tested ranged from 5 to 20 mg/mL. Ceftazidime-avibactam was tested at 8, 20, and 40 mg/mL, ceftolozane-tazobactam at 15 mg/mL, and piperacillin-tazobactam at 28 mg/mL. Compatibility of drug admixtures were tested via both simulated and actual y-site infusion. For the simulated y-site compatibility assessment, 1:1 mixtures of each respective drug were analyzed over 24 hours...
March 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/28430142/initial-spore-density-has-an-influence-on-ochratoxin-a-content-in-aspergillus-ochraceus-cgmcc-3-4412-in-pdb-and-its-interaction-with-seeds
#2
Caiyan Li, Yanmin Song, Lu Xiong, Kunlun Huang, Zhihong Liang
The morphology and secondary metabolism of Aspergillus spp. are associated with initial spore density (ISD). Fatty acids (FA) are involved in the biosynthesis of aflatoxins (AF) through Aspergillus quorum sensing (QS). Here, we studied how ochratoxin A (OTA) was regulated by spore density in Aspergillus ochraceus CGMCC 3.4412. The results contribute to understanding the role of spore density in morphogenesis, OTA biosynthesis, and host-pathogen interactions. When A. ochraceus was grown in Potato Dextrose Broth (PDB) media at different spore densities (from 10¹ to 10⁶ spores/mL), more OTA was produced when ISD were increased, but a higher level of ISD inhibited OTA biosynthesis...
April 21, 2017: Toxins
https://www.readbyqxmd.com/read/28421050/reveromycins-a-and-b-from-streptomyces-sp-3-10-antifungal-activity-against-plant-pathogenic-fungi-in-vitro-and-in-a-strawberry-food-model-system
#3
Ang Lyu, Hao Liu, Hongjie Che, Long Yang, Jing Zhang, Mingde Wu, Weidong Chen, Guoqing Li
This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3-10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3-10 was re-evaluated. The cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28411369/comparison-of-equine-platelet-function-and-survival-in-whole-blood-collected-in-acid-citrate-dextrose-solution-or-citrate-phosphate-dextrose-adenine-solution
#4
Rana Bozorgmanesh, Julie W Sutton-Burges, Fern Tablin
BACKGROUND: Equine whole blood collection and storage methods have been evaluated to assess red blood cell viability; however, platelet (PLT) viability has not been comprehensively assessed. OBJECTIVES: The purpose of the study was to compare viability of PLTs collected in whole blood into 2 different anticoagulants. METHODS: Whole blood from 6 healthy adult Thoroughbred horses was collected into citrate-phosphate-dextrose-adenine (CPDA) or acid-citrate-dextrose (ACD)...
April 14, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28297812/-the-regulative-effects-of-aspergillus-fumigatus-on-expression-of-glucocorticoid-receptor-in-asthmatic-rats
#5
X Zhou, Y J Dang, G F Wang, X Q Jin
Objective: To study the regulative effects of Aspergillus fumigatus (A.fumigatus) on expression of glucocorticoid receptor (GCR) in asthmatic rats. Methods: Wistar rats were randomly divided into 4 groups: a normal control group (UC), a normal control with A. fumigatus group (UC+ AF), an OVA group (OVA), and an OVA with A. fumigatus group (OVA+ AF). OVA and OVA+ AF groups were sensitized and challenged with OVA to establish asthmatic models. UC and UC+ AF groups were given normal saline as controls. After the last challenge, OVA+ AF and UC+ AF groups were given A...
March 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28291395/prospective-evaluation-of-intra-articular-dextrose-prolotherapy-for-treatment-of-osteoarthritis-in-dogs
#6
J Matthew Sherwood, James K Roush, Laura J Armbrust, Walter C Renberg
The objective of this study was to evaluate intra-articular dextrose prolotherapy for osteoarthritis of the elbow or stifle in dogs in a randomized, double-blind, placebo-controlled, prospective pilot study. Seventeen dogs were evaluated with 10 meeting inclusion criteria for this study. Evaluations included orthopedic exam, visual lameness scoring, Canine Brief Pain Inventory (CBPI), goniometry, kinetic gait analysis, and radiography. Initial lameness score, age, body weight, duration of lameness, and CBPI scores did not differ between groups...
March 14, 2017: Journal of the American Animal Hospital Association
https://www.readbyqxmd.com/read/28284926/moderate-short-term-local-hyperglycemia-attenuates-forearm-endothelium-dependent-vasodilation-after-transient-ischemia-reperfusion-in-human-volunteers
#7
Thomas J Ebert, Jutta Novalija, Jill A Barney, Toni D Uhrich, Shahbaz R Arain, Julie K Freed, Paul S Pagel
OBJECTIVE: Acute hyperglycemia causes endothelial dysfunction in diabetic patients, abolishes ischemic pre- and postconditioning, and is an independent predictor of adverse outcome after myocardial infarction in nondiabetic patients. Its effects on endothelial-dependent vasodilation are controversial in healthy subjects. The authors studied the effect of moderate short-term local hyperglycemia on forearm endothelium-dependent vasodilation in healthy volunteers. DESIGN: Randomized, crossover, blinded, 2-visit, pilot design...
December 2, 2016: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/28282803/blood-storage-alters-mechanical-stress-responses-of-erythrocytes
#8
Elif Ugurel, Zeynep Kucuksumer, Buse Eglenen, Ozlem Yalcin
BACKGROUND: Erythrocytes undergo irreversible morphological and biochemical changes during storage. Reduced levels of deformability have been reported for stored erythrocytes. Erythrocyte deformability is essential for healthy microcirculation. OBJECTIVE: The aim of this study is to evaluate shear stress (SS) induced improvements of erythrocyte deformability in stored blood. METHODS: Deformability changes were evaluated by applying physiological levels of SS (5 and 10 Pa) in metabolically depleted blood for 48 hours and stored blood for 35 days with citrate phosphate dextrose adenine-1 (CPDA-1)...
March 4, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28280761/comparison-of-candida-albicans-adherence-to-conventional-acrylic-denture-base-materials-and-injection-molding-acrylic-materials
#9
Masoomeh Aslanimehr, Shirin Rezvani, Ali Mahmoudi, Najmeh Moosavi
STATEMENT OF THE PROBLEM: Candida species are believed to play an important role in initiation and progression of denture stomatitis. The type of the denture material also influences the adhesion of candida and development of stomatitis. PURPOSE: The aim of this study was comparing the adherence of candida albicans to the conventional and injection molding acrylic denture base materials. MATERIALS AND METHOD: Twenty injection molding and 20 conventional pressure pack acrylic discs (10×10×2 mm) were prepared according to their manufacturer's instructions...
March 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28271330/protective-and-heat-retention-effects-of-thermo-sensitive-basement-membrane-extract-matrigel-in-hepatic-radiofrequency-ablation-in-an-experimental-animal-study
#10
Jing-Jing Fu, Song Wang, Wei Yang, Wei Gong, An-Na Jiang, Kun Yan, Min-Hua Chen
PURPOSE: To evaluate the protective effect of using thermo-sensitive basement membrane extract (Matrigel) for hydrodissection to minimize thermal injury to nearby structures and to evaluate its heat sink effect on the ablation zone in radiofrequency ablation (RFA) of the liver. MATERIALS AND METHODS: First, the viscosity profile and heat sink effect of Matrigel were assessed during RFA in vitro and ex vivo. Fresh pig liver tissue was used, and the temperature changes in Matrigel and in 5% dextrose in water (D5W) during RFA were recorded...
March 7, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28236651/intravenous-fluid-rate-for-reduction-of-cesarean-delivery-rate-in-nulliparous-women-a-systematic-review-and-meta-analysis
#11
REVIEW
Robert M Ehsanipoor, Gabriele Saccone, Neil S Seligman, Rebecca A M Pierce-Williams, Andrea Ciardulli, Vincenzo Berghella
INTRODUCTION: The National Institute of Child Health and Human Development, American College of Obstetricians and Gynecologists, and Society for Maternal-Fetal Medicine have emphasized the need to promote vaginal delivery and have offered recommendations to safely prevent primary cesarean delivery. However, there has been limited discussion regarding management of intravenous fluids and other aspects of labor management that may influence mode of delivery. Therefore the aim of our study was to determine whether an intravenous fluid rate of 250 vs...
February 25, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28223143/comparison-of-photoinactivation-of-t-rubrum-by-new-methylene-blue-nmb-and-indocyanine-green-emundo-%C3%A2
#12
Reza Fekrazad, Arash Poorsattar Bejeh Mir, Mina Kahyaie Aghdam, Vadood Ghasemi Barghi
BACKGROUND: Superficial mycotic skin infections which are predominantly caused by Trichophyton rubrum, poorly responses to conventional therapies. A great amount of attention has focused on to find more effective treatments. The current work is aimed to compare the effectiveness of phoinactivation of Trichophyton rubrum by two relatively new photosensitizers: a phenothiazinium dye(New methylene blue) and Indocyanine green (EmunDo(®)). MATERIALS AND METHODS: A Final inoculum of T...
February 18, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28215890/effect-of-oral-calcium-administration-on-the-cure-and-reproductive-performance-of-holstein-cows-diagnosed-with-puerperal-metritis
#13
Pablo Pinedo, Juan Velez, Gilberto Solano, Nelson Rodriguez, Julianne Naves, Gustavo M Schuenemann, Carlos Risco
Our objective was to evaluate the effect of oral calcium administration on clinical cure, survival, subsequent presentation of peripartal health disorders, and reproductive performance of Holstein cows diagnosed with puerperal metritis (PM) under certified organic management. A second objective was to evaluate the metabolic status at calving and at the time of PM diagnosis (d 0) in affected and matched healthy cows. Cows diagnosed with PM (n = 200) were assigned randomly to receive 1 of 2 treatments: (1) control received 3...
February 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28215169/bioactive-constituents-from-an-endophytic-fungus-penicillium-polonicum-nfw9-associated-with-taxus-fauna
#14
Nighat Fatima, Tawanun Sripisut, Ui Joung Youn, Safia Ahmed, Ihsan Ul-Haq, Ulyana Muñoz-Acuña, Charles J Simmons, Muneer Ahmed Qazi, Muniba Jadoon, Ghee Teng Tan, Esperanza J Carcache de Blanco, Leng Chee Chang
BACKGROUND: Endophytic fungi are being recognized as vital and untapped sources of a variety of structurally novel and unique bioactive secondary metabolites in the field of natural products drug discovery. Herein, this study reports the isolation and characterization of secondary metabolites from an endophytic fungus Penicillium polonicum (NFW9) associated with Taxus fuana. METHODS: Extracts of the endophytic fungus cultured on potato dextrose agar were purified using several chromatographic techniques...
February 16, 2017: Medicinal Chemistry
https://www.readbyqxmd.com/read/28205208/skin-to-skin-care-for-procedural-pain-in-neonates
#15
REVIEW
Celeste Johnston, Marsha Campbell-Yeo, Timothy Disher, Britney Benoit, Ananda Fernandes, David Streiner, Darlene Inglis, Rebekah Zee
BACKGROUND: Skin-to-skin care (SSC), often referred to as 'kangaroo care' (KC) due to its similarity with marsupial behaviour of ventral maternal-infant contact, is one non-pharmacological intervention for pain control in infants. OBJECTIVES: The primary objectives were to determine the effect of SSC alone on pain from medical or nursing procedures in neonates compared to no intervention, sucrose or other analgesics, or additions to simple SSC such as rocking; and to determine the effects of the amount of SSC (duration in minutes), method of administration (e...
February 16, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28164647/ethylenediaminetetraacetic-acid-sodium-citrate-heparin-and-citrate-phosphate-dextrose-adenine-anticoagulants-differentially-affect-cytokine-mrna-expression-in-blood-leukocytes
#16
Eva Herzogova, Pavlina Dankova
BACKGROUND: The aim of this study is to determine whether and how the anticoagulant agents EDTA, sodium citrate, and heparin present in vacutainers and CPDA located in transfusion bags modify peripheral blood cell behavior. METHODS: We compared the effect of four anticoagulants within an expression study quantifying mRNA by realtime PCR in 60 blood samples. RESULTS: We observed a significant increase of TNFα and CCL2 mRNA expression in leukocytes placed in EDTA anticoagulant compared to leukocytes collected with citrate or heparin (p ≤ 0...
July 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28141899/intravenous-midazolam-infusion-for-sedation-of-infants-in-the-neonatal-intensive-care-unit
#17
REVIEW
Eugene Ng, Anna Taddio, Arne Ohlsson
BACKGROUND: Proper sedation for neonates undergoing uncomfortable procedures may reduce stress and avoid complications. Midazolam is a short-acting benzodiazepine that is used increasingly in neonatal intensive care units (NICUs). However, its effectiveness as a sedative in neonates has not been systematically evaluated. OBJECTIVES: Primary objeciveTo assess the effectiveness of intravenous midazolam infusion for sedation, as evaluated by behavioural and/or physiological measurements of sedation levels, in critically ill neonates in the NICU...
January 31, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28121797/the-effect-of-postexercise-carbohydrate-and-protein-ingestion-on-bone-metabolism
#18
Rebecca Townsend, Kirsty J Elliott-Sale, Kevin Currell, Jonathan Tang, William D Fraser, Craig Sale
PURPOSE: To investigate the effect of feeding carbohydrate and protein (CHO+PRO), immediately or 2 h after an exhaustive run, on the bone turnover response in endurance runners. METHODS: 10 men (age 28±5 y, height 1.74±0.05 m, body mass 69.7±6.3 kg) performed treadmill running at 75%VO2max, until exhaustion, on three occasions. Blood was collected before and immediately, 1, 2, 3, 4 and 24 h post-exercise, for measurement of β-CTX, P1NP, PTH, PO4, ACa and Ca...
January 24, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28080896/traumatic-foreign-body-pericarditis-in-a-toco-toucan-ramphastos-toco
#19
Mireia Máinez, Jorge Rosell, Roger Such, Teresa Cardona, Carles Juan-Sallés
An approximately 10-yr-old, captive-born female toco toucan ( Ramphastos toco ) was presented due to an acute onset of depression and apathy. On visual and physical examination, it showed an abnormal posture and dehydration, respectively. Serum biochemistry revealed hyperuricemia (39.4 mg/dl) and elevated glutamic oxaloacetic transaminase (GOT; 1,050 U/L). Radiographs demonstrated an enlargement of the cardiac silhouette. The bird died 7 days after presentation, despite treatment with enrofloxacin, allopurinol, a preparation of hepatorenal protectors, and complex B vitamins with dextrose...
December 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28044994/influence-of-l-carnitine-on-stored-rat-blood-a-study-on-plasma
#20
Carl Hsieh, Vani Rajashekharaiah
OBJECTIVE: Plasma acts as a good indicator of oxidative stress in blood. L-carnitine is an antioxidant that reduces metabolic stress in cells, thereby providing a protective effect against oxidative stress. L-carnitine as an additive in storage has not been explored. Thus, this study attempts to analyze the role of L-carnitine in blood storage solution, Citrate Phosphate Dextrose Adenine (CPDA)-1 through oxidative stress (OS) markers - antioxidant enzymes, lipid peroxidation and protein oxidation...
December 30, 2016: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
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