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https://www.readbyqxmd.com/read/29122175/enhancing-taxol-production-in-a-novel-endophytic-fungus-aspergillus-aculeatinus-tax-6-isolated-from-taxus-chinensis-var-mairei
#1
Weichuan Qiao, Fei Ling, Lei Yu, Yifang Huang, Ting Wang
Taxol is a curatively effective but rare anti-tumor agent extracted from Taxus (yew) barks; however, the high cost and low production of the extraction method have limited its widespread use. In this study, fungi isolated from Taxus chinensis var. mairei were tested for their ability to produce taxol. High performance liquid chromatography combined with mass spectrometry confirmed that Aspergillus aculeatinus Tax-6, one of the endophytic fungi, could produce taxol in potato dextrose agar liquid medium. NaOAc, Cu(2+), and salicylic acid were introduced into the medium to enhance taxol production of strain Tax-6 because NaOAc is an important precursor of taxol, Cu(2+) may enhance the activity of oxidase and catalyze the formation of taxol, and salicylic acid could be an elicitor signal...
December 2017: Fungal Biology
https://www.readbyqxmd.com/read/29101774/dextrose-prolotherapy-for-chronic-shoulder-pain-a-case-report
#2
Casey Seenauth, Vance Inouye, Jeffrey O Langland
Context • Shoulder pain is one of the most common pain complaints reported by patients. Consensus is lacking on its ideal treatment, and many different treatments are available and used. Prolotherapy is an injection-based therapy that has shown significant results in treating many common musculoskeletal pain conditions, including osteoarthritis, lateral epicondylosis, and low-back pain. Objective • The study intended to evaluate the use of dextrose prolotherapy in the treatment of shoulder pain. Design • The research team performed a case study...
November 4, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/29101501/severe-hypernatraemic-dehydration-and-unconsciousness-in-a-care-dependent-inpatient-treated-with-empagliflozin
#3
Georg Gelbenegger, Nina Buchtele, Christian Schoergenhofer, Martin Roeggla, Michael Schwameis
A 66-year-old Caucasian male became unconscious 2 weeks after initiation of add-on therapy with empagliflozin for poorly controlled type 2 diabetes mellitus. The inpatient had recently suffered focal pontine stroke, rendering him bedridden and requiring increased nursing care, including assistance with drinking. The patient had received empagliflozin 10 mg once daily for glycaemic control. Investigations revealed hypernatraemia (164 mmol/l), a urine glucose level of 3935 mg/dl, and a creatinine level of 2...
November 3, 2017: Drug Safety—Case Reports
https://www.readbyqxmd.com/read/29076587/high-prevalence-of-candiduria-due-to-non-albicans-candida-species-among-diabetic-patients-a-matter-of-concern
#4
Alireza Esmailzadeh, Hossein Zarrinfar, AbdolMajid Fata, Tanuka Sen
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus...
October 27, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29066219/a-randomized-controlled-trial-of-one-bag-vs-two-bag-system-of-fluid-delivery-in-children-with-diabetic-ketoacidosis-experience-from-a-developing-country
#5
N Dhochak, M Jayashree, S Singhi
PURPOSE: To compare one vs. two bag system with respect to blood glucose variability (BGV), time for resolution of acidosis and incidence of hypoglycemia, hypokalemia, and cerebral edema in children with diabetic ketoacidosis (DKA). MATERIAL AND METHODS: In an open labelled randomized controlled trial, thirty consecutive patients ≤12years with DKA were randomized to either one (n=15) or two bag (n=15) system of intravenous fluid delivery. The two bags had similar electrolyte but differing dextrose concentration (none vs...
September 22, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/29017019/results-of-combined-single-session-arthrocentesis-and-dextrose-prolotherapy-for-symptomatic-temporomandibular-joint-syndrome-a-case-series
#6
Burak Cezairli, Efe Can Sivrikaya, Mehmet Melih Omezli, Ferhat Ayranci, Neslihan Seyhan Cezairli
OBJECTIVE: Arthrocentesis and prolotherapy are nonsurgical treatments for temporomandibular joint (TMJ) diseases. This study aimed to evaluate the treatment of hypermobility, pain, and displacement of the TMJ by consecutively performing arthrocentesis and prolotherapy in the same session. MATERIALS AND METHODS: In this study, 10 adults with disc displacement and painful, hypermobile TMJ were selected. Arthrocentesis and prolotherapy were consecutively performed using a 30% dextrose solution that was simultaneously injected into five areas: posterior disc attachment, superior joint space, superior and inferior capsular attachments, and stylomandibular ligament...
October 2017: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/28967446/-production-of-infectious-units-of-isaria-fumosorosea-hypocreales-cordycipitaceae-from-different-indigenous-isolates-of-northeastern-mexico-using-3-propagation-strategies
#7
Fatima L Gandarilla-Pacheco, Lilia H Morales-Ramos, Benito Pereyra-Alférez, Myriam Elías-Santos, Isela Quintero-Zapata
The aim of this study was to evaluate the production of blastospores and conidia of different native isolates and a strain of Isaria fumosorosea using different propagation techniques. Two liquid culture media of casamino acids and peptone as nitrogen sources and glucose as carbon source for both media cultures were respectively used in the production of blastospores, while for the production of conidia, the fungi were grown in potato dextrose agar; from these cultures, solutions of conidia to a concentration of 1×10(6) per milliliter were prepared to inoculate flasks with Sabouraud dextrose broth for the liquid phase of the biphasic culture, also known as preculture...
September 26, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28955647/purification-characterization-and-statistical-optimization-of-a-thermostable-%C3%AE-amylase-from-desert-actinobacterium-streptomyces-fragilis-da7-7
#8
Krishnasamy Nithya, Chinnasamy Muthukumar, Shine Kadaikunnan, Naiyf S Alharbi, Jamal M Khaled, Dharumadurai Dhanasekaran
In this study, preliminary screening revealed that of 134 desert soil actinobacterial isolates, only 43 isolates produced amylase. Among these, an isolate DA7-7, which was identified as Streptomyces fragilis DA7-7, showed a prominent zone of clearance and significant amount of α-amylase production. The pre-optimization studies showed varying physicochemical and nutrients properties of the medium influenced the enzyme production significantly. Consequently, central composite design was employed with the selected variables (pH, temperature, dextrose, and peptone) for α-amylase production...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28941697/spouted-bed-as-an-efficient-processing-for-probiotic-orange-juice-drying
#9
Niédila Nascimento Alves, Soraya de Oliveira Sancho, Ana Raquel Araújo da Silva, Stéphane Desobry, José Maria Correia da Costa, Sueli Rodrigues
This study evaluated the influence of spouted bed drying temperature and maltodextrin dextrose equivalent on the probiotic microbial survival during drying and storage period and on physicochemical properties of fermented probiotic orange juice in powder. Probiotic orange juice was spouted bed dried at 60, 70, 80 and 90°C using maltodextrin with a different dextrose equivalent (10, 20, 30 and 39). After drying, the microbial was higher when lower drying temperatures were applied. During the storage, the highest drying temperatures (80 and 90°C) negatively affected the microorganism survival...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28920043/short-term-analgesic-effects-of-5-dextrose-epidural-injections-for-chronic-low-back-pain-a-randomized-controlled-trial
#10
Liza Maniquis-Smigel, Kenneth Dean Reeves, Howard Jeffrey Rosen, John Lyftogt, Cassie Graham-Coleman, An-Lin Cheng, David Rabago
BACKGROUND: Hypertonic dextrose injection (prolotherapy) is reported to reduce pain including non-surgical chronic low back pain (CLBP), and subcutaneous injection of 5% dextrose is reported to reduce neurogenic pain, hyperalgesia and allodynia. The mechanism in both cases is unclear, though a direct effect of dextrose on neurogenic pain has been proposed. This study assessed the short-term analgesic effects of epidural 5% dextrose injection compared with saline for non-surgical CLBP...
February 2017: Anesthesiology and Pain Medicine
https://www.readbyqxmd.com/read/28901247/effect-on-morphology-osmotic-fragility-and-electro-kinetic-potential-of-erythrocytes-in-hypertension
#11
Swati Sanjay Gaikwad, J G Avari
BACKGROUND: Hypertension is a common health problem concerning a large proportion of population and a leading global risk factor for the burden of cardiovascular disease (CVD). The purpose of this study was to assess the efficacy of the erythrocyte zeta potential as a potential additional indicator for cardiovascular disorder risk so that patients with this can be more rapidly identified and treated. METHODS: In the present study, blood samples were collected in 5% dextrose solution from patients suffering from hypertension and healthy volunteers (Not taken any medication)...
September 11, 2017: Current Hypertension Reviews
https://www.readbyqxmd.com/read/28888602/properties-of-acid-gels-made-from-sodium-caseinate-maltodextrin-conjugates-prepared-by-a-wet-heating-method
#12
Shuwen Zhang, Yuansheng Gong, Som Khanal, Yanjie Lu, John A Lucey
Covalent attachment of polysaccharides to proteins (conjugation) via the Maillard reaction has been extensively studied. Conjugation can lead to a significant improvement in protein functionality (e.g., solubility, emulsification, and heat stability). Caseins have previously been successfully conjugated with maltodextrin (Md), but the effect on the detailed acid gelation properties has not been examined. We studied the effect of conjugating sodium caseinate (NaCN) with 3 different sized Md samples via the Maillard reaction in aqueous solutions...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28878933/comparison-of-a-two-bag-system-versus-conventional-treatment-protocol-one-bag-system-in-the-management-of-diabetic-ketoacidosis
#13
Iqbal Munir, Ramiz Fargo, Roger Garrison, Almira Yang, Andy Cheng, Ilho Kang, Ali Motabar, Karen Xu, Lawrence K Loo, Daniel I Kim
OBJECTIVE: We compared the conventional 'one-bag protocol' of management of diabetic ketoacidosis (DKA) with the 'two-bag protocol' which utilizes two bags of fluids, one containing saline and supplemental electrolytes and the other containing the same solution with the addition of 10% dextrose. RESEARCH DESIGN AND METHODS: A retrospective chart review and analysis was done on adult patients admitted for DKA to the Riverside University Health System Medical Center from 2008 to 2015...
2017: BMJ Open Diabetes Research & Care
https://www.readbyqxmd.com/read/28870480/propofol-sedation-substantially-increases-the-caloric-and-lipid-intake-in-critically-ill-patients
#14
Mélanie Charrière, Emma Ridley, Jennifer Hastings, Oliver Bianchet, Carlos Scheinkestel, Mette M Berger
OBJECTIVE: The amount of lipid delivered to patients varies considerably depending on the non-nutritional intake from sedation, and on the feeding solution. The aim of this study was to quantify the magnitude and proportion of lipids and energy provided from propofol sedation in intensive care unit (ICU) patients. METHODS: This was a retrospective analysis of prospectively collected data in consecutive patients admitted to the ICUs of two university hospitals. Inclusion criterion included an ICU stay >5 d...
October 2017: Nutrition
https://www.readbyqxmd.com/read/28854112/characterization-of-tolypocladium-cylindrosporum-hypocreales-ophiocordycipitaceae-and-its-impact-against-aedes-aegypti-and-aedes-albopictus-eggs-at-low-temperature
#15
Lina B Flor-Weiler, Alejandro P Rooney, Robert W Behle, Ephantus J Muturi
We examined the growth characteristics of Tolypocladium cylindrosporum IBT 41712 and its potential to infect eggs of Aedes aegypti and Ae. albopictus at a low temperature (15°C). When grown on Sabouraud dextrose agar supplemented with yeast extract, the IBT 41712 formed white colonies turning to a slightly darker, off-white color when mature. The mycelia bore swollen conidiophores producing smooth-walled, oblong to cylindrical conidia with varying sizes, ranging from 1.5 to 3.5 μm long. To determine the optimum temperature for the fungus, we cultured the fungus at eight temperatures (4°C, 12°C, 15°C, 21°C, 28°C, 33°C, 37°C, and 40°C) and measured the diametric growth...
September 2017: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28845775/preserving-and-maintaining-culinary-medicinal-royal-sun-mushroom-agaricus-brasiliensis-agaricomycetes-in-sterile-distilled-water
#16
L M Vargas-Del-Rlo, Sandra Montoya, J C Sepulveda-Arias
Strains of Agaricus brasiliensis require special procedures for conservation. Thus, the objective of this research was to establish conservation and maintenance procedures A. brasiliensis strain PSWC838 from the University of Pennsylvania (ABWC838) and an A. brasiliensis strain from the Fujian Agriculture and Forestry University (ABC). The medium in which mycelia grew the quickest for both strains was selected using a multifactorial design with 2 strains, 4 culture media, and incubation for 5 different times; the growth rate (mm/day) was the response variable...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28835147/characteristics-of-resveratrol-and-serotonin-on-antioxidant-capacity-and-susceptibility-to-oxidation-of-red-blood-cells-in-stored-human-blood-in-a-time-dependent-manner
#17
Zübeyir Huyut, Mehmet Ramazan Şekeroğlu, Ragıp Balahoroğlu, Mehmet Tahir Huyut
Objective In stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs. Methods Blood from each donor (n = 10) was placed in different bags containing 70 mL of citrate phosphate dextrose (total volume: 500 mL) and divided into three groups (n = 30): control, 60 µg/mL resveratrol, and 60 µg/mL serotonin...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28822458/-the-research-and-application-of-pretreatment-method-for-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-identification-of-filamentous-fungi
#18
Y F Huang, Z Chang, J Bai, M Zhu, M X Zhang, M Wang, G Zhang, X Y Li, Y G Tong, J L Wang, X X Lu
Objective: To establish and evaluate the feasibility of a pretreatment method for matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of filamentous fungi developed by the laboratory. Methods: Three hundred and eighty strains of filamentous fungi from January 2014 to December 2016 were recovered and cultured on sabouraud dextrose agar (SDA) plate at 28 ℃ to mature state. Meanwhile, the fungi were cultured in liquid sabouraud medium with a vertical rotation method recommended by Bruker and a horizontal vibration method developed by the laboratory until adequate amount of colonies were observed...
August 8, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28763254/decolorization-of-textile-dyes-in-an-air-lift-bioreactor-inoculated-with-bjerkandera-adusta-obr105
#19
Hong Sodaneath, Jung-In Lee, Seung-Ok Yang, Hyekyeng Jung, Hee Wook Ryu, Kyung-Suk Cho
A new decolorizing white-rot fungus, OBR105, was isolated from Mount Odae in South Korea and identified by the morphological characterization of its fruit body and spores and partial 18s rDNA sequences. The ligninolytic enzyme activity of OBR105 was studied to characterize their decolorizing mechanism using a spectrophotometric enzyme assay. For the evaluation of the decolorization capacity of OBR105, the isolate was incubated in an erlenmeyer flask and in an airlifte bioreator with potato dextrose broth (PDB) medium supplemented with each dye...
September 19, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28763003/short-term-effects-of-a-ready-to-drink-pre-workout-beverage-on-exercise-performance-and-recovery
#20
Patrick B Collins, Conrad P Earnest, Ryan L Dalton, Ryan J Sowinski, Tyler J Grubic, Christopher J Favot, Adriana M Coletta, Christopher Rasmussen, Mike Greenwood, Richard B Kreider
In a double-blind, randomized and crossover manner, 25 resistance-trained participants ingested a placebo (PLA) beverage containing 12 g of dextrose and a beverage (RTD) containing caffeine (200 mg), β-alanine (2.1 g), arginine nitrate (1.3 g), niacin (65 mg), folic acid (325 mcg), and Vitamin B12 (45 mcg) for 7-days, separated by a 7-10-day. On day 1 and 6, participants donated a fasting blood sample and completed a side-effects questionnaire (SEQ), hemodynamic challenge test, 1-RM and muscular endurance tests (3 × 10 repetitions at 70% of 1-RM with the last set to failure on the bench press (BP) and leg press (LP)) followed by ingesting the assigned beverage...
August 1, 2017: Nutrients
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