Lin Xie, Naotsugu Ichimaru, Miwa Morita, Jiajie Chen, Ping Zhu, Jihong Wang, Peter Urbanellis, Itay Shalev, Shizuko Nagao, Atsushi Sugioka, Liang Zhong, Norio Nonomura, Shiro Takahara, Gary A Levy, Xiao-Kang Li
Here we examined whether the expression of a novel immunoregulatory gene set could be used to predict outcomes in murine models of rapamycin-induced cardiac tolerance, spontaneous hepatic tolerance, and cardiac rejection. The expression of the immunoregulatory gene set was assessed with the GeXP multiplex reverse-transcription polymerase chain reaction (RT-PCR) analysis system, and it was correlated to the pathological and biochemical parameters of the allografts. In rejecting cardiac grafts, the increased expression of an inflammatory set of genes, which included CD45, CD4, CD25, suppressor of cytokine signaling 2, cytotoxic T lymphocyte-associated protein 4 (CTLA4), selectin lymphocyte, interferon-γ (IFN-γ), programmed cell death 1 (Pdcd1), and granzyme B (Gzmb), was seen 8 days after transplantation along with histological evidence of severe allograft rejection...
April 2012: Liver Transplantation