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https://www.readbyqxmd.com/read/28924661/correlative-sim-storm-microscopy
#1
O Burri, T Laroche, R Guiet, A Seitz
The ability to specifically label subcellular structures or even proteins of interest in combination with the ability to look at live specimens turned fluorescence light microscopy into an invaluable tool. However, conventional light microscopy is diffraction limited, which restricts the lateral resolution to around 200 nm laterally and 600-800 nm axially. In 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, Stefan W. Hell, and William E. Moerner for the development of super-resolved fluorescent microscopy techniques...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28923763/hemispheric-specialization-for-global-and-local-processing-a-direct-comparison-of-linguistic-and-non-linguistic-stimuli
#2
Sanne G Brederoo, Mark R Nieuwenstein, Monicque M Lorist, Frans W Cornelissen
It is often assumed that the human brain processes the global and local properties of visual stimuli in a lateralized fashion, with a left hemisphere (LH) specialization for local detail, and a right hemisphere (RH) specialization for global form. However, the evidence for such global-local lateralization stems predominantly from studies using linguistic stimuli, the processing of which has shown to be LH lateralized in itself. In addition, some studies have reported a reversal of global-local lateralization when using non-linguistic stimuli...
September 15, 2017: Brain and Cognition
https://www.readbyqxmd.com/read/28922876/visual-deficit-from-laser-interstitial-thermal-therapy-for-temporal-lobe-epilepsy-anatomical-considerations
#3
Walter J Jermakowicz, Michael E Ivan, Iahn Cajigas, Ramses Ribot, Ignacio Jusue-Torres, Mehul B Desai, Armando Ruiz, Pierre-Francois D'Haese, Andres M Kanner, Jonathan R Jagid
BACKGROUND: Laser interstitial thermal therapy (LITT) is quickly emerging as an effective surgical therapy for temporal lobe epilepsy (TLE). One of the most frequent complications of the procedure is postoperative visual field cuts, but the physiopathology of these deficits is unknown. OBJECTIVE: To evaluate potential causes of visual deficits after LITT for TLE in an attempt to minimize this complication. METHODS: This retrospective chart review compares the case of a 24-year-old male who developed homonymous hemianopsia following LITT for TLE to 17 prior patients who underwent the procedure and suffered no visual deficit...
October 1, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/28919034/a-sensitive-and-specific-lateral-flow-assay-for-rapid-detection-of-antibodies-against-glycoprotein-b-of-aujeszky-s-disease-virus
#4
Veronika V Vrublevskaya, Vladimir N Afanasyev, Andrey A Grinevich, Yuri Y Skarga, Pavel P Gladyshev, Sagila A Ibragimova, Dmitry V Krylsky, Sergey V Dezhurov, Oleg S Morenkov
A direct double antibody lateral flow assay (DDA-gB-LFA) for the detection of antibodies against the glycoprotein B (gB) of Aujeszky's disease virus (ADV) in swine sera was developed. A native ADV gB was used for the preparation of a conjugate with colloidal gold particles and the immobilization on the strip membrane. The gB purified from ADV virions by immunoaffinity chromatography retained its native epitope structure after adsorption on the nitrocellulose membrane and the surface of colloidal gold particles...
September 14, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28916470/receptive-field-properties-of-cat-perigeniculate-neurons-correlate-with-excitatory-and-inhibitory-connectivity-to-lgn-relay-neurons
#5
Hironobu Osaki, Tomoyuki Naito, Shogo Soma, Hiromichi Sato
The cat perigeniculate nucleus (PGN) is a visual sector of the thalamic reticular nucleus that consists of GABAergic neurons. It receives excitatory axon-collateral input from relay neurons of the dorsal lateral geniculate nucleus (LGN) to which it provides inhibitory input. Thus, it is usually argued that the PGN works as feedback inhibition to the LGN. At the single neuron level, however, this circuit can also provide lateral inhibition. Which inhibition dominates in the visual circuit of the thalamus has yet to be well characterized...
September 12, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28893999/cortically-coordinated-nrem-thalamocortical-oscillations-play-an-essential-instructive-role-in-visual-system-plasticity
#6
Jaclyn Durkin, Aneesha K Suresh, Julie Colbath, Christopher Broussard, Jiaxing Wu, Michal Zochowski, Sara J Aton
Two long-standing questions in neuroscience are how sleep promotes brain plasticity and why some forms of plasticity occur preferentially during sleep vs. wake. Establishing causal relationships between specific features of sleep (e.g., network oscillations) and sleep-dependent plasticity has been difficult. Here we demonstrate that presentation of a novel visual stimulus (a single oriented grating) causes immediate, instructive changes in the firing of mouse lateral geniculate nucleus (LGN) neurons, leading to increased firing-rate responses to the presented stimulus orientation (relative to other orientations)...
September 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28891856/transanal-total-mesorectal-excision-pneumodissection-of-retroperitoneal-structures-eases-laparoscopic-rectal-resection
#7
Cristiano G S Hüscher, Marco Maria Lirici
INTRODUCTION: Laparoscopic total mesorectal excision is effective and safe but often technically challenging because of inadequate exposure. Transanal total mesorectal excision was introduced to mitigate this limitation and improve the quality of mesorectal dissection in even the most challenging cases. Currently, the technique for transanal total mesorectal excision dissection is not standardized. TECHNIQUE: The sequential approach to transanal total mesorectal excision mirrors the principles of the transanal abdominal transanal procedure...
October 2017: Diseases of the Colon and Rectum
https://www.readbyqxmd.com/read/28889002/mapping-the-complex-topological-organization-of-the-human-parietal-face-area
#8
Ruey-Song Huang, Ching-Fu Chen, Martin I Sereno
The macaque monkey ventral intraparietal area (VIP) contains neurons with aligned visual-tactile receptive fields anchored to the face and upper body. Our previous fMRI studies using standard head coils found a human parietal face area (VIP + complex; putative macaque VIP homologue) containing superimposed topological maps of the face and near-face visual space. Here, we construct high signal-to-noise surface coils and used phase-encoded air puffs and looming stimuli to map topological organization of the parietal face area at higher resolution...
September 6, 2017: NeuroImage
https://www.readbyqxmd.com/read/28871810/mammography-positioning-standards-in-the-digital-era-is-the-status-quo-acceptable
#9
Ashley I Huppe, Kelly L Overman, Jason B Gatewood, Jacqueline D Hill, Louise C Miller, Marc F Inciardi
OBJECTIVE: The objective of our study was to evaluate positioning of full-field digital mammography (FFDM) and digital breast tomosynthesis (DBT) compared with film-screen (FS) mammography positioning standards. MATERIALS AND METHODS: A retrospective study was conducted of consecutive patients who underwent screening FFDM in 2010-2012 and DBT in 2012-2013 at an academic institution. Examinations were performed by five experienced technologists who underwent updated standardized positioning training...
September 5, 2017: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/28871030/spatiochromatic-interactions-between-individual-cone-photoreceptors-in-the-human-retina
#10
William S Tuten, Wolf M Harmening, Ramkumar Sabesan, Austin Roorda, Lawrence C Sincich
A remarkable feature of human vision is that the retina and brain have evolved circuitry to extract useful spatial and spectral information from signals originating in a photoreceptor mosaic whose trichromatic constituents vary widely in their relative numbers and local spatial configurations. A critical early transformation applied to cone signals is horizontal-cell-mediated lateral inhibition, which imparts a spatially-antagonistic surround to individual cone receptive fields, a signature inherited by downstream neurons and implicated in color signaling...
September 4, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28867482/neurochemical-differences-betweentarget-specific-populations-of-rat-dorsal-raphe-projection-neurons
#11
Eric W Prouty, Daniel J Chandler, Barry D Waterhouse
Serotonin (5-HT)-containing neurons in the dorsal raphe (DR) nucleus project throughout the forebrain and are implicated in many physiological processes and neuropsychiatric disorders. Diversity among these neurons has been characterized in terms of their neurochemistry and anatomical organization, but a clear sense of whether these attributes align with specific brain functions or terminal fields is lacking. DR 5-HT neurons can co-express additional neuroactive substances, increasing the potential for individualized regulation of target circuits...
August 31, 2017: Brain Research
https://www.readbyqxmd.com/read/28867207/lesions-of-the-head-direction-cell-system-increase-hippocampal-place-field-repetition
#12
Bruce Harland, Roddy M Grieves, David Bett, Rachael Stentiford, Emma R Wood, Paul A Dudchenko
A central tenet of systems neuroscience is that the mammalian hippocampus provides a cognitive map of the environment. This view is supported by the finding of place cells, neurons whose firing is tuned to specific locations in an animal's environment, within this brain region. Recent work, however, has shown that these cells repeat their firing fields across visually identical maze compartments [1, 2]. This repetition is not observed if these compartments face different directions, suggesting that place cells use a directional input to differentiate otherwise similar local environments [3, 4]...
September 11, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28865060/gut-contents-digestive-half-lives-and-feeding-state-prediction-in-the-soil-predatory-mite-pergamasus-longicornis-mesostigmata-parasitidae
#13
Clive E Bowman
Mid- and hind-gut lumenal changes are described in the free-living predatory soil mite Pergamasus longicornis (Berlese) from a time series of histological sections scored during and after feeding on fly larval prey. Three distinct types of tangible material are found in the lumen. Bayesian estimation of the change points in the states of the gut lumenal contents over time is made using a time-homogenous first order Markov model. Exponential processes within the gut exhibit 'stiff' dynamics. A lumen is present throughout the midgut from 5 min after the start of feeding as the gut rapidly expands...
September 1, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28863111/the-endoscopic-prelacrimal-recess-approach-to-the-pterygopalatine-fossa-and-infratemporal-fossa
#14
Li Gao, Lei Zhou, Zhengshou Dai, Xinsheng Huang
PURPOSE: The authors studied the anatomic importance of the endoscopic prelacrimal recess approach (PLRA) to the pterygopalatine fossa (PPF) and infratemporal fossa (ITF). METHODS: Ten adult heads (20 sides) from cadavers fixed in formalin were dissected using the PLRA. Anatomic dissections were detailed and several crucial landmarks measured. RESULTS: Identification of the infraorbital neurovascular bundle is the crucial step for the detection of other branches of the maxillary artery...
September 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28855858/processing-of-unattended-emotional-facial-expressions-correlates-of-visual-field-bias-in-women
#15
Dina Wittfoth, Christine Preibisch, Heinrich Lanfermann
Lateralization in emotional processing is a matter of ongoing debate. Various factors can influence lateralized emotional processing, including stimulus location, emotional valence, and gender. In the present study, we aim to elucidate how unattended emotional facial expressions shown at different locations in the visual field influence behavioral responses, eye movement, and neural responses in a sample of healthy women. Our female participants viewed fearful, happy and neutral faces presented at central and peripheral (left or right) locations while keeping their gaze locked on a central fixation crosshairs and indicating stimulus location via button presses...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28842389/usability-of-a-culturally-informed-mhealth-intervention-for-symptoms-of-anxiety-and-depression-feedback-from-young-sexual-minority-men
#16
John B Fleming, Yvette N Hill, Michelle Nicole Burns
BACKGROUND: To date, we are aware of no interventions for anxiety and depression developed as mobile phone apps and tailored to young sexual minority men, a group especially at risk of anxiety and depression. We developed TODAY!, a culturally informed mobile phone intervention for young men who are attracted to men and who have clinically significant symptoms of anxiety or depression. The core of the intervention consists of daily psychoeducation informed by transdiagnostic cognitive behavioral therapy (CBT) and a set of tools to facilitate putting these concepts into action, with regular mood ratings that result in tailored feedback (eg, tips for current distress and visualizations of mood by context)...
August 25, 2017: JMIR Human Factors
https://www.readbyqxmd.com/read/28837233/real-time-tomosynthesis-for-radiation-therapy-guidance
#17
Scott S Hsieh, Lydia W Ng
PURPOSE: Fluoroscopy has been a tool of choice for monitoring treatments or interventions because of its extremely fast imaging times. However, the contrast obtained in fluoroscopy may be insufficient for certain clinical applications. In stereotactic ablative radiation therapy of the lung, fluoroscopy often lacks sufficient contrast for gating treatment. The purpose of this work is to describe and assess a real-time tomosynthesis design that can produce sufficient contrast for guidance of lung tumor treatment within a small field of view...
August 24, 2017: Medical Physics
https://www.readbyqxmd.com/read/28835530/rapid-visuomotor-feedback-gains-are-tuned-to-the-task-dynamics
#18
Sae Franklin, Daniel M Wolpert, David W Franklin
Adaptation to novel dynamics requires learning a motor memory, or a new pattern of predictive feedforward motor commands. Recently we demonstrated the up-regulation of rapid visuomotor feedback gains early in curl force field learning, which decrease once a predictive motor memory is learned. However, even after learning is complete, these feedback gains are higher than those observed in the null field trials. Interestingly these up-regulated feedback gains in the curl field were not observed in a constant force field...
August 23, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28832724/selection-of-alfalfa-genotypes-for-resistance-to-the-foliar-pathogen-curvularia-geniculata
#19
Mariana R Ávila, Miguel Dall'agnol, José A Martinelli, Gerarda B P DA Silva, Carolina Bremm, Tamyris Nunes
Foliar diseases impose severe restrictions on the persistence and productivity of Medicago sativa, both of which may be increased by developing disease resistant and more competitive genotypes that can improve pasture quality. We found Curvularia geniculata as the principal alfalfa foliar pathogen in the Brazilian state of Rio Grande do Sul (RS). Growth chamber experiments evaluated the resistance of alfalfa genotypes 'E1C4', 'CPPSul', 'ABT 805' and 'CUF-101' to C. geniculata as compared the control 'Crioula' genotype...
August 17, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28827673/field-friendly-serological-tests-for-determination-of-m-leprae-specific-antibodies
#20
Anouk van Hooij, Elisa M Tjon Kon Fat, Susan J F van den Eeden, Louis Wilson, Moises Batista da Silva, Claudio G Salgado, John S Spencer, Paul L A M Corstjens, Annemieke Geluk
Early detection of leprosy is key to reduce the ongoing transmission. Antibodies directed against M. leprae PGL-I represent a useful biomarker for detecting multibacillary (MB) patients. Since efficient leprosy diagnosis requires field-friendly test conditions, we evaluated two rapid lateral flow assays (LFA) for detection of Mycobacterium leprae-specific antibodies: the visual immunogold OnSite Leprosy Ab Rapid test [Gold-LFA] and the quantitative, luminescent up-converting phosphor anti-PGL-I test [UCP-LFA]...
August 21, 2017: Scientific Reports
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