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https://www.readbyqxmd.com/read/28215509/triphenylphosphonium-modified-poly-ethylene-glycol-poly-%C3%AE%C2%B5-caprolactone-micelles-for-mitochondria-targeted-gambogic-acid-delivery
#1
Yingqi Xu, Shengpeng Wang, Hon Fai Chan, Yuling Liu, Hui Li, Chengwei He, Zeyong Li, Meiwan Chen
Mitochondria are important targets for the intracellular delivery of drugs and DNA. For mitochondria-targeted delivery, a mitochondriotropic molecule, triphenylphosphonium (TPP), was applied to the synthesis of amphiphilic TPP-poly(ethylene glycol)-poly(ε-caprolactone) (TPP-PEG-PCL) polymers. The TPP-PEG-PCL polymer was used to prepare micelles using a solvent evaporation method for the delivery of gambogic acid (GA) (GA-TPP). The micelles were obtained with a favorable particle size of 150.07±11.71nm and an encapsulation efficiency of 80...
February 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28208246/combined-proteome-and-eicosanoid-profiling-approach-for-revealing-implications-of-human-fibroblasts-in-chronic-inflammation
#2
Ammar Tahir, Andrea Bileck, Besnik Muqaku, Laura Niederstaetter, Dominique Kreutz, Rupert L Mayer, Denise Wolrab, Samuel M Meier, Astrid Slany, Christopher Gerner
During inflammation, proteins and lipids act in a concerted fashion, calling for combined analyses. Fibroblasts are powerful mediators of chronic inflammation. However, little is known about eicosanoid formation by human fibroblasts. The aim of this study was to analyze the formation of the most relevant inflammation mediators including proteins and lipids in human fibroblasts upon inflammatory stimulation and subsequent treatment with dexamethasone, a powerful antiphlogistic drug. Label-free quantification was applied for proteome profiling, while an in-house established data-dependent analysis method based on high-resolution mass spectrometry was applied for eicosadomics...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28207247/ortho-methoxyphenols-as-convenient-oxidative-bioconjugation-reagents-with-application-to-site-selective-heterobifunctional-crosslinkers
#3
Adel M ElSohly, James Irving MacDonald, Nina B Hentzen, Ioana Laura Aanei, Kareem M El Muslemany, Matthew B Francis
The synthesis of complex protein-based bioconjugates has been facilitated greatly by recent developments in chemoselective methods for biomolecular modification. The oxidative coupling of o-aminophenols or catechols with aniline functional groups is chemoselective, mild, and rapid; however, the oxidatively sensitive nature of the electron-rich aromatics and the paucity of commercial sources pose some obstacles to the general use of these reactive strategies. Herein, we identify o-methoxyphenols as air-stable, commercially available derivatives that undergo efficient oxidative couplings with anilines in the presence of periodate as oxidant...
February 16, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28195470/total-synthesis-confirms-the-molecular-structure-proposed-for-oxidized-levuglandin-d2
#4
Yu-Shiuan Cheng, Wenyuan Yu, Yunfeng Xu, Robert G Salomon
Levuglandins (LG)D2 and LGE2 are γ-ketoaldehyde levulinaldehyde derivatives with prostanoid side chains produced by spontaneous rearrangement of the endoperoxide intermediate PGH2 in the biosynthesis of prostaglandins. Covalent adduction of LGs with the amyloid peptide Aβ1-42 promotes formation of the type of oligomers that have been associated with neurotoxicity and are a pathologic hallmark of Alzheimer's disease. Within 1 min of their generation during the production of PGH2 by cyclooxygenation of arachidonic acid, LGs are sequestered by covalent adduction to proteins...
February 14, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28183860/diminished-satellite-cell-fusion-and-s6k1-expression-in-myotubes-derived-from-skeletal-muscle-of-low-birth-weight-neonatal-pigs
#5
Ying Chen, Haibo Zhu, Sydney R McCauley, Lidan Zhao, Sally E Johnson, Robert P Rhoads, Samer W El-Kadi
Low birth weight (LBWT) is consistently associated with impaired postnatal muscle growth in mammals. Satellite cell (SC)-mediated myonuclear incorporation precedes protein accumulation in the early stages of postnatal muscle development and growth. The objective of this study was to investigate proliferation and differentiation of SCs and the regulation of protein synthesis signaling in response to insulin-like growth factor (IGF)-I stimulation in SC-derived myotubes of LBWT neonatal pigs. SCs isolated from Longissimus dorsi muscle of LBWT and NBWT pigs (3-d-old, n = 8) were cultured and induced to proliferate and differentiate to myotubes in vitro...
February 2017: Physiological Reports
https://www.readbyqxmd.com/read/28183064/quantitative-proteomics-analysis-reveals-perturbation-of-lipid-metabolic-pathways-in-the-liver-of-atlantic-cod-gadus-morhua-treated-with-pcb-153
#6
Fekadu Yadetie, Eystein Oveland, Anne Døskeland, Frode Berven, Anders Goksøyr, Odd André Karlsen
PCB 153 is one of the most abundant PCB congeners detected in biological samples. It is a persistent compound that is still present in the environment despite the ban on production and use of PCBs in the late 1970s. It has strong tendencies to bioaccumulate and biomagnify in biota, and studies have suggested that it is an endocrine and metabolic disruptor. In order to study mechanisms of toxicity, we exposed Atlantic cod (Gadus morhua) to various doses of PCB 153 (0, 0.5, 2 and 8mg/kg body weight) for two weeks and examined the effects on expression of liver proteins using label-free quantitative proteomics...
January 30, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28181828/synthesis-of-sericin-based-conjugates-by-click-chemistry-enhancement-of-sunitinib-bioavailability-and-cell-membrane-permeation
#7
Luca Scrivano, Domenico Iacopetta, Maria Stefania Sinicropi, Carmela Saturnino, Pasquale Longo, Ortensia Ilaria Parisi, Francesco Puoci
Sericin is a natural protein that has been used in biomedical and pharmaceutical fields as raw material for polypeptide-based drug delivery systems (DDSs). In this paper, it has been employed as pharmaceutical biopolymer for the production of sunitinib-polypeptide conjugate. The synthesis has been carried out by simple click reaction in water, using the redox couple l-ascorbic acid/hydrogen peroxide as a free radical grafting initiator. The bioconjugate molecular weight (50 kDa < Mw < 75 kDa) was obtained by SDS-PAGE, while the spectroscopic characteristics have been studied in order to reveal the presence of grafted sunitinib...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28168121/effects-of-rhodomyrtone-on-gram-positive-bacterial-tubulin-homologue-ftsz
#8
Dennapa Saeloh, Michaela Wenzel, Thanyada Rungrotmongkol, Leendert Willem Hamoen, Varomyalin Tipmanee, Supayang Piyawan Voravuthikunchai
Rhodomyrtone, a natural antimicrobial compound, displays potent activity against many Gram-positive pathogenic bacteria, comparable to last-defence antibiotics including vancomycin and daptomycin. Our previous studies pointed towards effects of rhodomyrtone on the bacterial membrane and cell wall. In addition, a recent molecular docking study suggested that the compound could competitively bind to the main bacterial cell division protein FtsZ. In this study, we applied a computational approach (in silico), in vitro, and in vivo experiments to investigate molecular interactions of rhodomyrtone with FtsZ...
2017: PeerJ
https://www.readbyqxmd.com/read/28165370/plumbagin-suppresses-%C3%AE-msh-induced-melanogenesis-in-b16f10-mouse-melanoma-cells-by-inhibiting-tyrosinase-activity
#9
Taek-In Oh, Jeong-Mi Yun, Eun-Ji Park, Young-Seon Kim, Yoon-Mi Lee, Ji-Hong Lim
Recent studies have shown that plumbagin has anti-inflammatory, anti-allergic, antibacterial, and anti-cancer activities; however, it has not yet been shown whether plumbagin suppresses alpha-melanocyte stimulating hormone (α-MSH)-induced melanin synthesis to prevent hyperpigmentation. In this study, we demonstrated that plumbagin significantly suppresses α-MSH-stimulated melanin synthesis in B16F10 mouse melanoma cells. To understand the inhibitory mechanism of plumbagin on melanin synthesis, we performed cellular or cell-free tyrosinase activity assays and analyzed melanogenesis-related gene expression...
February 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28155937/controlled-aggregation-and-cell-uptake-of-thermoresponsive-polyoxazoline-grafted-superparamagnetic-iron-oxide-nanoparticles
#10
Steffen Kurzhals, Noga Gal, Ronald Zirbs, Erik Reimhult
Hydrophilic polymer-coated iron oxide nanoparticles are potential materials for a plethora of applications in the biotechnological field. Typical such polymers, e.g. dextran or poly(ethylene glycol), lack the ability to tailor the biological response to an environmental trigger, while common responsive polymers such as poly(N-isopropylacrylamide) or poly(acrylic acid) are not suitable for biomedical applications. We present the synthesis and characterization of superparamagnetic iron oxide nanoparticles with thermoresponsive polyoxazoline brushes grafted at unprecedented density using nitrodopamine anchor chemistry...
February 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28154371/oxidative-stress-and-calcium-dysregulation-by-palmitate-in-type-2-diabetes
#11
REVIEW
Luong Dai Ly, Shanhua Xu, Seong-Kyung Choi, Chae-Myeong Ha, Themis Thoudam, Seung-Kuy Cha, Andreas Wiederkehr, Claes B Wollheim, In-Kyu Lee, Kyu-Sang Park
Free fatty acids (FFAs) are important substrates for mitochondrial oxidative metabolism and ATP synthesis but also cause serious stress to various tissues, contributing to the development of metabolic diseases. CD36 is a major mediator of cellular FFA uptake. Inside the cell, saturated FFAs are able to induce the production of cytosolic and mitochondrial reactive oxygen species (ROS), which can be prevented by co-exposure to unsaturated FFAs. There are close connections between oxidative stress and organellar Ca(2+) homeostasis...
February 3, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28149525/self-assembled-peg-b-pdpa-b-pgem-copolymer-nanoparticles-as-protein-antigen-delivery-vehicles-to-dendritic-cells-preparation-characterization-and-cellular-uptake
#12
Pan Li, Junhui Zhou, Pingsheng Huang, Chuangnian Zhang, Weiwei Wang, Chen Li, Deling Kong
Antigen uptake by dendritic cells (DCs) is a key step for initiating antigen-specific T cell immunity. In the present study, novel synthetic polymeric nanoparticles were prepared as antigen delivery vehicles to improve the antigen uptake by DCs. Well-defined cationic and acid-responsive copolymers, monomethoxy poly(ethylene glycol)-block-poly(2-(diisopropyl amino) ethyl methacrylate)-block-poly(2-(guanidyl) ethyl methacrylate) (mPEG-b-PDPA-b-PGEM, PEDG) were synthesized by reversible addition-fragmentation chain transfer polymerization of 2-(diisopropylamino)ethyl methacrylate) and N-(tert-butoxycarbonyl) amino ethyl methacrylate monomers, followed by deprotection of tert-butyl protective groups and guanidinylation of obtained primary amines...
February 2017: Regenerative Biomaterials
https://www.readbyqxmd.com/read/28139884/streptomyces-venezuelae-tx-tl-a-next-generation-cell-free-synthetic-biology-tool
#13
Simon J Moore, Hung-En Lai, Hannah Needham, Karen M Polizzi, Paul S Freemont
Streptomyces venezuelae is a promising chassis in synthetic biology for fine chemical and secondary metabolite pathway engineering. The potential of S. venezuelae could be further realized by expanding its capability with the introduction of its own in vitro transcription-translation (TX-TL) system. TX-TL is a fast and expanding technology for bottom-up design of complex gene expression tools, biosensors and protein manufacturing. Herein, we introduce a S. venezuelae TX-TL platform by reporting a streamlined protocol for cell-extract preparation, demonstrating high-yield synthesis of a codon-optimized sfGFP reporter and the prototyping of a synthetic tetracycline-inducible promoter in S...
January 31, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28127897/cell-free-translational-screening-of-an-expression-sequence-tag-library-of-clonorchis-sinensis-for-novel-antigen-discovery
#14
Devi Kasi, Christy Catherine, Seung-Won Lee, Kyung-Ho Lee, Yu Jung Kim, Myeong Ro Lee, Jung Won Ju, Dong-Myung Kim
The rapidly evolving cloning and sequencing technologies have enabled understanding of genomic structure of parasite genomes, opening up new ways of combatting parasite-related diseases. To make the most of the exponentially accumulating genomic data, however, it is crucial to analyze the proteins encoded by these genomic sequences. In this study, we adopted an engineered cell-free protein synthesis system for large-scale expression screening of an expression sequence tag (EST) library of Clonorchis sinensis to identify potential antigens that can be used for diagnosis and treatment of clonorchiasis...
January 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28123046/pde5-inhibitor-tadalafil-and-hydroxychloroquine-co-treatment-provides-synergistic-protection-against-type-2-diabetes-and-myocardial-infarction-in-mice
#15
Rui Wang, Lei Xi, Rakesh C Kukreja
Diabetes is associated with high risk of ischemic heart disease. We previously showed that phosphodiesterase 5 inhibitor - tadalafil (TAD) induces cardioprotection against ischemia/ reperfusion (I/R) injury in diabetic mice. Hydroxychloroquine (HCQ) is a widely used antimalarial and anti-inflammatory drug, which was reported to reduce hyperglycemia in diabetic patients. Therefore we hypothesized that combination of TAD and HCQ may induce synergistic cardioprotection in diabetes. We also investigated the role of insulin-Akt-mTOR signaling, which regulates protein synthesis and cell survival...
January 25, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28114298/single-molecule-detection-of-protein-efflux-from-microorganisms-using-fluorescent-single-walled-carbon-nanotube-sensor-arrays
#16
Markita Patricia Landry, Hiroki Ando, Allen Y Chen, Jicong Cao, Vishal Isaac Kottadiel, Linda Chio, Darwin Yang, Juyao Dong, Timothy K Lu, Michael S Strano
A distinct advantage of nanosensor arrays is their ability to achieve ultralow detection limits in solution by proximity placement to an analyte. Here, we demonstrate label-free detection of individual proteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic chamber, measuring protein efflux from single organisms in real time. The array is fabricated using non-covalent conjugation of an aptamer-anchor polynucleotide sequence to near-infrared emissive single-walled carbon nanotubes, using a variable chemical spacer shown to optimize sensor response...
January 23, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28113560/biomedical-and-catalytic-applications-of-gold-and-silver-gold-alloy-nanoparticles-biosynthesized-using-cell-free-extract-of-bacillus-safensis-lau-13-antifungal-dye-degradation-anti-coagulant-and-thrombolytic-activities
#17
Sunday A Ojo, Agbaje Lateef, Musibau A Azeez, Suliat M Oladejo, Abiola S Akinwale, Tesleem B Asafa, Taofeek A Yekeen, Akeem Akinboro, Iyabo C Oladipupo, Evariste B Gueguim-Kana, Lorika S Beukes
This study investigated the green biosynthesis of gold (Au) and silver-gold alloy (Ag-Au) nanoparticles using cell-free extract of Bacillus safensis LAU 13 strain (GenBank accession No: KJ461434). The biosynthesized AuNPs and Ag-AuNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared spectroscopy, and Transmission electron microscopy. Evaluation of the antifungal activities, degradation of malachite green, anti-coagulation of blood and thrombolysis of human blood clot by the biosynthesized nanoparticles were investigated...
April 29, 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28112394/establishing-a-high-yielding-streptomyces-based-cell-free-protein-synthesis-system
#18
Jian Li, He Wang, Yong-Chan Kwon, Michael C Jewett
Cell-free protein synthesis (CFPS) has emerged as a powerful platform for applied biotechnology and synthetic biology, with a range of applications in synthesizing proteins, evolving proteins, and prototyping genetic circuits. To expand the current CFPS repertoire, we report here the development and optimization of a Streptomyces-based CFPS system for the expression of GC-rich genes. By developing a streamlined crude extract preparation protocol and optimizing reaction conditions, we were able to achieve active enhanced green fluorescent protein (EGFP) yields of greater than 50 µg/mL with batch reactions lasting up to 3 h...
January 23, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28112179/dicer1-mir-29-hmgcr-axis-contributes-to-hepatic-free-cholesterol-accumulation-in-mouse-non-alcoholic-steatohepatitis
#19
Ming-Xia Liu, Man Gao, Chun-Zhu Li, Cun-Zhi Yu, Hong Yan, Chun Peng, Yu Li, Cheng-Gang Li, Ze-Long Ma, Yang Zhao, Meng-Fan Pu, Ling-Ling Miao, Xin-Ming Qi, Jin Ren
Dicer1 is an enzyme essential for microRNA (miRNA) maturation. The loss of miRNAs resulted from Dicer1 deficiency greatly contributes to the progression of many diseases, including lipid dysregulation, but its role in hepatic accumulation of free cholesterol (FC) that is critical in the development of non-alcoholic steatohepatitis (NASH) remains elusive. In this study, we used the liver-specific Dicer1-knockout mice to identify the miRNAs involved in hepatic FC accumulation. In a widely used dietary NASH model, mice were fed a methionine-choline-deficient (MCD) diet for 3 weeks, which resulted in significant increase in hepatic FC levels as well as decrease of Dicer1 mRNA levels in livers...
January 23, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28107519/cudraflavone-c-induces-tumor-specific-apoptosis-in-colorectal-cancer-cells-through-inhibition-of-the-phosphoinositide-3-kinase-pi3k-akt-pathway
#20
Hsien-Chuen Soo, Felicia Fei-Lei Chung, Kuan-Hon Lim, Veronica Alicia Yap, Tracey D Bradshaw, Ling-Wei Hii, Si-Hoey Tan, Sze-Jia See, Yuen-Fen Tan, Chee-Onn Leong, Chun-Wai Mai
Cudraflavone C (Cud C) is a naturally-occurring flavonol with reported anti-proliferative activities. However, the mechanisms by which Cud C induced cytotoxicity have yet to be fully elucidated. Here, we investigated the effects of Cud C on cell proliferation, caspase activation andapoptosis induction in colorectal cancer cells (CRC). We show that Cud C inhibits cell proliferation in KM12, Caco-2, HT29, HCC2998, HCT116 and SW48 CRC but not in the non-transformed colorectal epithelial cells, CCD CoN 841. Cud C induces tumor-selective apoptosis via mitochondrial depolarization and activation of the intrinsic caspase pathway...
2017: PloS One
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