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https://www.readbyqxmd.com/read/28527297/hyaluronic-acid-nanogels-prepared-via-ortho-ester-linkages-show-ph-triggered-behavior-enhanced-penetration-and-antitumor-efficacy-in-3-d-tumor-spheroids
#1
Guanqing Yang, Shengxiang Fu, Weijing Yao, Xin Wang, Qian Zha, Rupei Tang
A new type of pH-triggered hyaluronic acid nanogel system (HA-NGs) was successfully developed for tumor-targeted delivery of drugs. HA-NGs were obtained by copolymerization between methacrylate HA and a new cross-linker containing ortho ester groups in an aqueous solution. The therapeutic drug (DOX) was loaded into the HA-NGs (DOX@HA-NGs) and exhibited appropriate loading of about 17.3% with a size of around 200nm. Such new pH-triggered HA-NGs are found to be highly desirable for targeted cancer therapy because it could significantly minimize the amount of premature drug release in neutral pH, and also provide a sufficient amount of drug to effectively kill the cancer cells caused by the degradation of ortho ester groups at acid pH values...
May 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28526689/long-non-coding-rna-neat1-promotes-hepatocellular-carcinoma-cell-proliferation-through-the-regulation-of-mir-129-5p-vcp-i%C3%AE%C2%BAb
#2
Luo Fang, Jiao Sun, Zongfu Pan, Yu Song, Like Zhong, Yiwen Zhang, Yujia Liu, Xiaowei Zheng, Ping Huang
BACKGROUND: Long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) plays an important role in the pathogenesis and development of several types of cancer. However, the functional mechanism of NEAT1 in hepatocellular carcinoma (HCC) remains unclear. METHODS: The NEAT1 and miR-129-5p expression in HCC tissues and cell lines were quantified by means of qPCR. The effects of NEAT1 expression inhibition or up-regulation in HCC cell lines were analyzed in terms of cell viability and apoptosis...
May 19, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28526602/palmitoyl-ascorbate-and-doxorubicin-co-encapsulated-liposome-for-synergistic-anticancer-therapy
#3
Yue Yang, Xiaoyu Lu, Qi Liu, Yu Dai, Xiaojie Zhu, Yanli Wen, Jiaqiu Xu, Yang Lu, Di Zhao, Xijing Chen, Ning Li
Combination therapy with two drugs and nanoparticle-based drug delivery systems are widely applied to reduce the adverse effects of traditional treatment by chemotherapeutic drugs. Palmitoyl ascorbate (PA) as a lipophilic derivative of ascorbic acid shows the advantages in cancer treatment. The aim of the study was to prepare a doxorubicin (DOX) and PA co-loaded liposome to synergistically treat tumor and effectively alleviate the toxicity caused by DOX. The effects were evaluated by in vitro and in vivo studies...
May 16, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28526426/the-effects-of-troglitazone-on-ampk-in-hepg2-cells
#4
Katherine M Allen, Kimberly A Coughlan, Fabliha N Mahmood, Rudy J Valentine, Neil B Ruderman, Asish K Saha
AMP-activated protein kinase (AMPK) is an enzyme crucial in cellular metabolism found to be inhibited in many metabolic diseases including type 2 diabetes. Thiazolidinediones (TZDs) are a class of anti-diabetic drug known to activate AMPK through increased phosphorylation at Thr(172), however there has been no research to date on whether they have any effect on inhibition of AMPK's lesser known site of inhibition, Ser(485/491). HepG2 cells were treated with troglitazone and phosphorylation of AMPK was found to increase at both Thr(172) and Ser(485) in a dose- and time-dependent manner...
May 16, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28526339/magnesium-isoglycyrrhizinate-blocks-fructose-induced-hepatic-nf-%C3%AE%C2%BAb-nlrp3-inflammasome-activation-and-lipid-metabolism-disorder
#5
Xiao-Juan Zhao, Yan-Zi Yang, Yan-Jing Zheng, Shan-Chun Wang, Hong-Mei Gu, Ying Pan, Shui-Juan Wang, Hong-Jiang Xu, Ling-Dong Kong
Magnesium isoglycyrrhizinate as a hepatoprotective agent possesses immune modulation and anti-inflammation, and treats liver diseases. But its effects on immunological-inflammatory and metabolic profiles for metabolic syndrome with liver injury and underlying potential mechanisms are not fully understood. In this study, magnesium isoglycyrrhizinate alleviated liver inflammation and lipid accumulation in fructose-fed rats with metabolic syndrome. It also suppressed hepatic inflammatory signaling activation by reducing protein levels of phosphorylation of nuclear factor-kappa B p65 (p-NF-κB p65), inhibitor of nuclear factor kappa-B kinase α/β (p-IKKα/β) and inhibitor of NF-κB α (p-IκBα) as well as nucleotide-binding domain (NOD)-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein (ASC) and Caspase-1 in rats, being consistent with its reduction of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-6 levels...
May 16, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28525815/an-integrated-approach-to-study-the-risk-from-landfill-soil-of-delhi-chemical-analyses-in-vitro-assays-and-human-risk-assessment
#6
Swati, Pooja Ghosh, Indu Shekhar Thakur
In the present study, landfill soil of three municipal solid waste landfill sites of Delhi, India were toxico-chemically analyzed for human risk assessment as inadequate information is available on the possible health effects of the contaminants present in landfill soil. The landfill soil samples were prepared for analyzing heavy metal concentration, organic contaminants and toxicity analysis separately. Composite soil sample collected from three landfill sites were analyzed for heavy metal by ICP-AES. Metal concentration so obtained was below the permissible limit of soil but higher than the set limits for effluent...
May 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28525794/stat6-silencing-induces-hepatocellular-carcinoma-derived-cell-apoptosis-and-growth-inhibition-by-decreasing-the-rankl-expression
#7
Tian Qing, Zhang Yamin, Wang Guijie, Jin Yan, Shen Zhongyang
Signal transducer and activator of transcription-6 (STAT6) is highly expressed in various human cancers and considered a regulator of multiple biological processes in cancers, including cell apoptosis. Evidence has indicated that STAT6 predicts a worse prognosis in hepatocellular carcinoma (HCC) patients. The objective of this study was to investigate the effects and mechanism of STAT6 in human HCC cells. We found that STAT6 silencing significantly inhibited HepG2 and Hep3B cell survival and proliferation. We observed that depletion of STAT6 increased HepG2 and Hep3B cell apoptosis by using a histone DNA ELISA detection kit...
May 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28525367/liposomal-co-delivered-oleanolic-acid-attenuates-doxorubicin-induced-multi-organ-toxicity-in-hepatocellular-carcinoma
#8
Muhammad Sarfraz, Attia Afzal, Shahid Masood Raza, Sajid Bashir, Asadullah Madni, Muhammad Waseem Khan, Xiang Ma, Guangya Xiang
Doxorubicin in combination with other cytotoxic drugs has clinical advantages. However, doxorubicin-induced cardiotoxicity negatively impacts clinical utility and outcomes. Cardiotoxicity can result from increased oxidative stress or from a local cytochrome P450 mediated increase in 20-hydroxy-5, 8, 11, 14-eicosatetraenoic acid (20-HETE). Oleanolic acid (OA) is a natural pentacyclic triterpenoid with free radical scavenging, cardioprotective, and P450-mediated cyclooxygenase-upregulating properties. We investigated co-delivery of liposomal OA and doxorubicin in a HepG2 model of hepatocellular carcinoma (HCC)...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28524760/evaluation-of-the-cytotoxicity-hepg2-and-chemical-composition-of-polar-extracts-from-the-ruderal-species-coleostephus-myconis-l-rchb-f
#9
Sílvia M F Bessada, João C M Barreira, J Santos, Carla Costa, Filipa B Pimentel, Maria João Bessa, João Paulo Teixeira, M Beatriz P P Oliveira
Coleostephus myconis (L.) Rchb.f. (Asteraceae) is a highly disseminated plant species with ruderal and persistent growth. Owing to its advantageous agronomic properties, C. myconis might have industrial applications. However, this species needs to be comprehensively characterized before any potential use. In a previous study, the phenolic composition and antioxidant activity of different C. myconis tissues were characterized. This investigation was extended to examine the cytotoxic potential of selected plant tissues (flowers and green parts) using a HepG2 cell line by utilizing the lysosomal neutral red uptake assay or mitochondrial (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay...
May 19, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28523535/self-protection-against-triptolide-induced-toxicity-in-human-hepatic-cells-via-nrf2-are-nqo1-pathway
#10
Ling-Ling Zhou, Cong Zhou, Xiao-Wen Liang, Zhe Feng, Zhang-Pu Liu, Hao-Lu Wang, Xue-Ping Zhou
OBJECTIVE: To find the signaling pathway of triptolide (TP)-induced liver injury and to reveal whether NF-E2-related factor 2 (Nrf2) plays an important role in cellular self-protection. METHODS: The L-02 and HepG2 cells were cultured and treated with various concentrations of TP. The cell viability was observed, and the cell medium was collected for detecting the aspartate aminotransferase (ALT), alanine aminotransferase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and L-glutathione production (GSH) levels...
May 18, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28522265/screening-for-bioactive-natural-products-from-a-67-compound-library-of-glycyrrhiza-inflata
#11
Yan Lin, Yi Kuang, Kai Li, Shuang Wang, Wei Song, Xue Qiao, Gulnar Sabir, Min Ye
Licorice shows a variety of pharmacological activities. This work aims to discover bioactive natural products from one botanical source of licorice, Glycyrrhiza inflata. A total of 67 free phenolics were isolated to form a compound library. Based on the bioactivities of licorice, these compounds were screened using cell- or enzyme-based bioassay methods. A total of 11 compounds exhibited potent cytotoxic activities against three human cancer cell lines (HepG2, SW480 and MCF7), while showed little toxicity on human normal cell lines LO2 and HEK293T...
May 6, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28522194/aptamer-mediated-gene-therapy-enhanced-antitumor-activity-against-human-hepatocellular-carcinoma-in-vitro-and-in-vivo
#12
Shuangli Xiao, Zhongbing Liu, Ruolan Deng, Chunhong Li, Shaozhi Fu, Guilan Chen, Xiaoqin Zhang, Famin Ke, Siyun Ke, Xin Yu, Shurong Wang, Zhirong Zhong
A recombinant adenovirus carrying the tumor suppressor gene PTEN (Ad5-PTEN) is an effective antitumor agent against liver cancer. But the application of Ad5-PTEN has been greatly hindered by its auto-immunogenicity, non-specific toxicity to normal tissues, as well as poor stability in blood stream because of neutralizing antibody. Epithelial cell adhesion molecule (EpCAM) is over-expressed in most solid tumors and it has been identified to be a cancer stem cell surface marker in liver cancer. An RNA aptamer EpDT3 could specially bind with EpCAM and target EpCAM-positive cells...
May 15, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28522001/cytotoxic-effect-of-a-mannogalactoglucan-extracted-from-agaricus-bisporus-on-hepg2-cells
#13
Amanda do Rocio Andrade Pires, Andrea Caroline Ruthes, Silvia Maria Suter Correia Cadena, Marcello Iacomini
A mannogalactoglucan (RK2-Ab; Mw 1.8×10(4)gmol(-1)) composed by Man (27.3%), Gal (24.4%) and Glc (48.3%) was extracted and characterized from Agaricus bisporus, and its biological activity was evaluated on human hepatocarcinoma cells (HepG2). The partially-O-methylated alditol acetates together with the NMR data suggest the main chain to be composed of α-d-Galp (32.8%) and β-d-Glcp (37.0%) units (1→6)-linked, with β-d-Manp (14.6%), as non-reducing end units, substituting the side chains at O-2 (α-d-Galp units; 3...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521864/insulin-induced-inhibition-of-gluconeogenesis-genes-including-glutamic-pyruvic-transaminase-2-is-associated-with-reduced-histone-acetylation-in-a-human-liver-cell-line
#14
Kazue Honma, Michiko Kamikubo, Kazuki Mochizuki, Toshinao Goda
OBJECTIVES: Hepatic glutamic pyruvic transaminase (GPT; also known as alanine aminotransferase) is a gluconeogenesis enzyme that catalyzes conversions between alanine and pyruvic acid. It is also used as a blood biomarker for hepatic damage. In this study, we investigated whether insulin regulates GPT expression, as it does for other gluconeogenesis genes, and if this involves the epigenetic modification of histone acetylation. METHODS: Human liver-derived HepG2 cells were cultured with 0...
June 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28521610/sphk1-sphingosine-kinase-1-induces-epithelial-mesenchymal-transition-by-promoting-the-autophagy-linked-lysosomal-degradation-of-cdh1-e-cadherin-in-hepatoma-cells
#15
Hong Liu, Yan Ma, Hong-Wei He, Wu-Li Zhao, Rong-Guang Shao
SPHK1 (sphingosine kinase 1), a regulator of sphingolipid metabolites, plays a causal role in the development of hepatocellular carcinoma (HCC) through augmenting HCC invasion and metastasis. However, the mechanism by which SPHK1 signaling promotes invasion and metastasis in HCC remains to be clarified. Here, we reported that SPHK1 induced the epithelial-mesenchymal transition (EMT) by accelerating CDH1/E-cadherin lysosomal degradation and facilitating the invasion and metastasis of HepG2 cells. Initially, we found that SPHK1 promoted cell migration and invasion and induced the EMT process through decreasing the expression of CDH1, which is an epithelial marker...
May 4, 2017: Autophagy
https://www.readbyqxmd.com/read/28521450/atmospheric-particulate-matter2-5-promotes-the-migration-and-invasion-of-hepatocellular-carcinoma-cells
#16
Qian Zhang, Qiong Luo, Xueyu Yuan, Li Chai, Dan Li, Jianjun Liu, Zhongwei Lv
Epidemiological data has demonstrated that particulate matter (PM) with an aerodynamic diameter ≤ 2.5 µm (PM2.5) is associated with cancer incidence. However, the precise mechanisms underlying PM2.5-mediated hepatocellular carcinoma cancer (HCC) migration and invasion remain unclear. The aim of the present study was to explore the response of the HCC cell lines HepG2 and HuH-7 to PM2.5 exposure. The results revealed that PM2.5 treatment promoted the migration and invasion of HCC cells, in addition to increasing protein levels of matrix metalloproteinase (MMP)-13...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28521273/rapid-discovery-of-cyclopamine-analogs-from-fritillaria-and-veratrum-plants-using-lc-q-tof-ms-and-lc-qqq-ms
#17
Yuan Du, Zu-Guo Zheng, Yue Yu, Zi-Tian Wu, Dan Liang, Ping Li, Yan Jiang, Hui-Jun Li
Cyclopamine, an inhibitor of the Hedgehog (Hh) signaling pathway, has been paid much attention in treating a wide variety of tumors. However, isolation and purification of cyclopamine analogs from medicinal plants remain challengeable. We herein proposed an efficient strategy using liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) and liquid chromatography triple-quadrupole mass spectrometry (LC-QqQ-MS) for rapid screening and targeted isolation of cyclopamine analogs in Fritillaria and Veratrum plants...
May 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28521254/cs-peg-decorated-plga-nano-prototype-for-delivery-of-bioactive-compounds-a-novel-approach-for-induction-of-apoptosis-in-hepg2-cell-line
#18
Ahmed A Abd-Rabou, Hanaa H Ahmed
PURPOSE: Polymer-based nanoparticles are used as vectors for cancer drug delivery. The bioactive compounds (quercetin, ellagic acid and gallic acid) are well known to be not only antioxidants but also chemopreventive candidates against various types of cancers. To circumvent the low bioavailability and the short half-life time obstacles, we hypothesized a novel PLGA nano-platform functionalized with CS and PEG to encapsulate these phytochemicals. This encapsulation will protect the compounds from the phagocytic uptake and deliver PLGA-CS-PEG nano-prototype with high biodegradability and biosafety...
March 12, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28520759/first-evidence-of-the-hepatitis-e-virus-in-environmental-waters-in-colombia
#19
Paula A Baez, Maria Camila Lopez, Alejandra Duque-Jaramillo, Dioselina Pelaez, Francisco Molina, Maria-Cristina Navas
Hepatitis E virus (HEV) is one of the main causes of acute viral hepatitis of enteric transmission. HEV has been detected in environmental samples in several countries from Europe and Asia, constituting a risk factor for waterborne infection. In Colombia, HEV has been identified in samples obtained from patients as well as from swine, but no environmental studies have been carried out. To determine if HEV is present in environmental waters, samples from the main source of drinking water plant and of wastewater system of eight municipalities and two villages of Antioquia state (North West Colombia), were collected between December 2012 and April 2014...
2017: PloS One
https://www.readbyqxmd.com/read/28520529/synthesis-of-theranostic-epithelial-cell-adhesion-molecule-targeted-mesoporous-silica-nanoparticle-with-gold-gatekeeper-for-hepatocellular-carcinoma
#20
Maryam Babaei, Khalil Abnous, Seyed Mohammad Taghdisi, Sara Amel Farzad, Mohammad Taghi Peivandi, Mohammad Ramezani, Mona Alibolandi
AIM: In this study, we report the fabrication of epithelial cell adhesion molecule targeted 5-fluorouracil (5-FU) encapsulated PEGylated mesoporous silica nanoparticles (NPs) hybridized with gold NPs (PEG-Au@Si-5-FU) as gatekeeper for theranostic applications. MATERIALS & METHODS: The prepared targeted and nontargeted formulations were evaluated in vitro in terms of their cellular internalization and toxicity. The prepared theranostic hybrid system was also implemented for computed tomography of HepG2 tumor-bearing nude mice in vivo...
May 18, 2017: Nanomedicine
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