keyword
https://read.qxmd.com/read/38529613/analytical-characterization-of-heterogeneities-in-mrna-lipid-nanoparticles-using-sucrose-density-gradient-ultracentrifugation
#1
JOURNAL ARTICLE
Amita Vaidya, Dipen Parande, Nikita Khadse, Natalia Vargas-Montoya, Vikram Agarwal, Christian Ortiz, Gordon Ellis, Neha Kaushal, Ashish Sarode, Shrirang Karve, Frank DeRosa
Rational design and robust formulation processes are critical for optimal delivery of mRNA by lipid nanoparticles (LNPs). Varying degrees of heterogeneity in mRNA-LNPs can affect their biophysical and functional properties. Given the profound complexity of mRNA-LNPs, it is critical to develop comprehensive and orthogonal analytical techniques for a better understanding of these formulations. To this end, we developed a robust ultracentrifugation method for density-based separation of subpopulations of mRNA-LNPs...
March 26, 2024: Analytical Chemistry
https://read.qxmd.com/read/38528249/positive-response-of-a-hemodialysis-patient-with-pure-red-cell-aplasia-on-recombinant-human-erythropoietin-therapy-to-cyclosporine-and-roxadustat
#2
JOURNAL ARTICLE
Xuejuan Ma, Pearl Pai, Wenjuan Zhu, Xiaowei Chen, Liwen Cui
Recombinant human erythropoietin (rHuEPO) is commonly used to treat anemia associated with chronic kidney disease (CKD). EPO-induced Pure Red Cell Aplasia (PRCA) is a rare condition of profound anemia with EPO treatment. Upon finding the development of EPO-induced PRCA, the treatment requires immediate withdrawal of EPO therapy and initiate new treatments with immunosuppression or renal transplantation. Anti-EPO antibody assay is not always positive in EPO-induced PRCA. Here, we report a case on the sudden development of PRCA in a hemodialysis patient receiving EPO and how we treated the condition successfully with cyclosporine and subsequently maintained the hemoglobin with Roxadustat, a hypoxia-inducible factor prolyl hydroxylase inhibitor (HIF-PHI)...
March 25, 2024: CEN Case Reports
https://read.qxmd.com/read/38523044/consensus-of-the-brazilian-association-of-hematology-hemotherapy-and-cellular-therapy-on-patient-blood-management-preoperative-phase-preoperative-management-of-the-patient-s-anemia
#3
JOURNAL ARTICLE
Bruno Deltreggia Benites, Flavia Leite, Selma Soriano, Roberto Luiz da Silva, Susankerle de Oliveira Costa Alves, Silvia Renata Cornélio Parolin Rizzo, Guilherme Rabello, Dante Mario Langhi Junior
Managing anemia before surgery is extremely important as it is a clinical condition that can significantly increase surgical risk and affect patient outcomes. Anemia is characterized by a reduction in the number of red blood cells or hemoglobin levels leading to a lower oxygen-carrying capacity of the blood. Proper treatment requires a multifaceted approach to ensure patients are in the best possible condition for surgery and to minimize potential complications. The challenge is recognizing anemia early and implementing a timely intervention to correct it...
March 11, 2024: Hematology, Transfusion and Cell Therapy
https://read.qxmd.com/read/38519416/effects-of-erythropoietin-on-cognitive-impairment-and-prefrontal-cortex-activity-across-affective-disorders-a-randomized-double-blinded-placebo-controlled-trial
#4
JOURNAL ARTICLE
Julian Macoveanu, Jeff Zarp Petersen, Johanna Mariegaard, Andreas Elleby Jespersen, Katrine Cramer, Caroline Fussing Bruun, Helle Østergaard Madsen, Martin Balslev Jørgensen, Maj Vinberg, Patrick M Fisher, Gitte Moos Knudsen, Ida Hageman, Hannelore Ehrenreich, Lars Vedel Kessing, Kamilla Woznica Miskowiak
BACKGROUND: Persistent cognitive impairment is frequent across bipolar disorder (BD) and major depressive disorder (MDD), highlighting an urgent need for pro-cognitive treatments. AIM: This study investigated effects of erythropoietin (EPO) on cognitive impairment and dorsal prefrontal cortex (dPFC) activity in affective disorders. METHODS: In this randomized, double-blinded, placebo-controlled trial, cognitively impaired patients with remitted BD or MDD received 1 weekly recombinant human EPO (40,000 IU/mL) or saline infusion for a 12-week period...
March 22, 2024: Journal of Psychopharmacology
https://read.qxmd.com/read/38518606/inhibition-the-ubiquitination-of-enac-and-na-k-atpase-with-erythropoietin-promotes-alveolar-fluid-clearance-in-sepsis-induced-acute-respiratory-distress-syndrome
#5
JOURNAL ARTICLE
Ye Gao, Fei Cao, Xinyi Tian, Qianping Zhang, Congcong Xu, Bowen Ji, Ye-An Zhang, Linan Du, Jun Han, Li Li, Siyu Zhou, Yuqiang Gong, Binyu Ying, Fang Gao-Smith, Shengwei Jin
Sepsis-induced acute respiratory distress syndrome (ARDS) causes significant fatalities worldwide and lacks pharmacological intervention. Alveolar fluid clearance (AFC) plays a pivotal role in the remission of ARDS and is markedly impaired in the pathogenesis of ARDS. Here, we demonstrated that erythropoietin could effectively ameliorate lung injury manifestations and lethality, restore lung function and promote AFC in a rat model of lipopolysaccharide (LPS)-induced ARDS. Moreover, it was proven that EPO-induced restoration of AFC occurs through triggering the total protein expression of ENaC and Na,K-ATPase channels, enhancing their protein abundance in the membrane, and suppressing their ubiquitination for degeneration...
March 21, 2024: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38514743/erythropoietin-and-retinopathy-of-prematurity-a-retrospective-cohort-study-in-japan-2008-2018
#6
JOURNAL ARTICLE
Kana Fukui, Yushi Ito, Masayo Kokubo, Hidehiko Nakanishi, Shinya Hirano, Satoshi Kusuda, Shuichi Ito, Tetsuya Isayama
BACKGROUND: Erythropoietin has an angiogenic effect on the retina and might increase the risk of retinopathy of prematurity (ROP). METHODS: This retrospective cohort study included infants born at 22 to 27 weeks' gestation between 2008 and 2018 who were admitted to neonatal intensive care units (NICUs). We compared mortality and morbidities between infants who received erythropoietin and those who did not. RESULTS: Among 18,955 livebirth infants, this study included 16,031 infants, among which 14,373 infants received erythropoietin...
March 21, 2024: Journal of Perinatology: Official Journal of the California Perinatal Association
https://read.qxmd.com/read/38511749/updated-threshold-renewed-problems-should-the-diagnostic-criteria-of-polycythemia-vera-be-reconsidered-a-retrospective-cross-sectional-cohort-study
#7
JOURNAL ARTICLE
Anıl Uçan, Müfide Okay Özgeyik
OBJECTIVE: This aim of this study was to evaluate hemoglobin and hematocrit values of polycythemia vera and secondary polycythemia patients with updated World Health Organization thresholds. In addition, by determining our own threshold values, we aimed to demonstrate the necessity of bone marrow biopsy and genetic analysis to be used for further diagnosis in patients with high-normal hematocrit and hemoglobin values. METHODS: A cross-sectional and retrospective study was performed with the medical records of patients from Eskisehir City Hospital hematology clinics and outpatient clinics between July 1, 2019 and July 1, 2020...
2024: Revista da Associação Médica Brasileira
https://read.qxmd.com/read/38509561/activating-mutations-in-jak2-and-calr-differentially-affect-intracellular-calcium-flux-in-store-operated-calcium-entry
#8
JOURNAL ARTICLE
Vikas Bhuria, Tobias Franz, Conny Baldauf, Martin Böttcher, Nicolas Chatain, Steffen Koschmieder, Tim H Brümmendorf, Dimitrios Mougiakakos, Burkhart Schraven, Sascha Kahlfuß, Thomas Fischer
BACKGROUND: Calcium (Ca2+ ) signaling regulates various vital cellular functions, including integrin activation and cell migration. Store-operated calcium entry (SOCE) via calcium release-activated calcium (CRAC) channels represents a major pathway for Ca2+ influx from the extracellular space in multiple cell types. The impact of JAK2-V617F and CALR mutations which are disease initiating in myeloproliferative neoplasms (MPN) on SOCE, calcium flux from the endoplasmic reticulum (ER) to the cytosol, and related key signaling pathways in the presence or absence of erythropoietin (EPO) or thrombopoietin (TPO) is poorly understood...
March 21, 2024: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/38509204/erythropoietin-receptor-signal-is-crucial-for-periodontal-ligament-stem-cell-based-tissue-reconstruction-in-periodontal-disease
#9
JOURNAL ARTICLE
Mhd Fouad Zakaria, Soichiro Sonoda, Hiroki Kato, Lan Ma, Norihisa Uehara, Yukari Kyumoto-Nakamura, M Majd Sharifa, Liting Yu, Lisha Dai, Erika Yamauchi-Tomoda, Reona Aijima, Haruyoshi Yamaza, Fusanori Nishimura, Takayoshi Yamaza
Alveolar bone loss caused by periodontal disease eventually leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are the tissue-specific cells for maintaining and repairing the periodontal ligament, cementum, and alveolar bone. Here, we investigated the role of erythropoietin receptor (EPOR), which regulates the microenvironment-modulating function of mesenchymal stem cells, in PDLSC-based periodontal therapy. We isolated PDLSCs from patients with chronic periodontal disease and healthy donors, referred to as PD-PDLSCs and Cont-PDLSCs, respectively...
March 20, 2024: Scientific Reports
https://read.qxmd.com/read/38508605/myomatous-erythrocytosis-syndrome-a-uterine-fibroid-associated-with-polycythaemia
#10
JOURNAL ARTICLE
Faisal Ansari, Talal Al Assil, Mohammad Omaira, Anna V Hoekstra
Myomatous erythrocytosis syndrome (MES) is a rare form of secondary erythrocytosis seen with myomas. Here, we present a case of a postmenopausal, nulliparous woman in her 50s incidentally found to have asymptomatic erythrocytosis on routine laboratory work. She was found to have an 18.5 cm myoma and after surgical resection, the patient's haematological values returned to normal ranges after a few weeks. This established the diagnosis as MES. The aetiology of MES continues to remain unknown but is most likely caused by an autonomous production of erythropoietin from the myomatous tissue...
March 19, 2024: BMJ Case Reports
https://read.qxmd.com/read/38504497/gene-doping-control-analysis-of-human-erythropoietin-transgene-in-equine-plasma-by-pcr-liquid-chromatography-high-resolution-tandem-mass-spectrometry
#11
JOURNAL ARTICLE
Bruce Pui-Nam Yuen, Kin-Sing Wong, Yat-Ming So, Wai Him Kwok, Hiu Wing Cheung, Terence See Ming Wan, Emmie Ngai-Man Ho, Wing-Tak Wong
Gene doping involves the misuse of genetic materials to alter an athlete's performance, which is banned at all times in both human and equine sports. Quantitative polymerase chain reaction (qPCR) assays have been used to control the misuse of transgenes in equine sports. Our laboratory recently developed and implemented duplex as well as multiplex qPCR assays for transgenes detection. To further advance gene doping control, we have developed for the first time a sensitive and definitive PCR-liquid chromatography high-resolution tandem mass spectrometry (PCR-LC-HRMS/MS) method for transgene detection with an estimated limit of detection of below 100 copies/mL for the human erythropoietin (hEPO) transgene in equine plasma...
March 19, 2024: Analytical Chemistry
https://read.qxmd.com/read/38501358/predicting-the-long-term-effects-of-therapeutic-neutralization-of-oncostatin-m-on-human-hematopoiesis
#12
JOURNAL ARTICLE
Anders Thorsted, Chiara Zecchin, Alienor Berges, Mats O Karlsson, Lena E Friberg
Therapeutic neutralization of Oncostatin M (OSM) causes mechanism-driven anemia and thrombocytopenia, which narrows the therapeutic window complicating the selection of doses (and dosing intervals) that optimize efficacy and safety. We utilized clinical data from studies of an anti-OSM monoclonal antibody (GSK2330811) in healthy volunteers (n = 49) and systemic sclerosis patients (n = 35), to quantitatively determine the link between OSM and alterations in red blood cell (RBC) and platelet production...
March 19, 2024: Clinical Pharmacology and Therapeutics
https://read.qxmd.com/read/38496140/refractory-ischemic-priapism-following-hemodialysis
#13
Abdalhai Alshoubi, Farris Al-Qawasmi
Priapism, characterized by prolonged and painful penile erection, is a rare urological emergency with diverse etiologies. We present a case of refractory ischemic priapism following hemodialysis in a 57-year-old male with a history of type II diabetes mellitus, hypertension, and end-stage renal disease. Despite standard conservative management, the patient's condition persisted, necessitating penile distal shunting through an intracorporeal dilatation plus Al-Ghorab corporoglandular shunt. Blood gas analysis of corpus cavernosum blood revealed severe acidosis and hypoxemia, emphasizing the systemic impact of ischemic priapism...
February 2024: Curēus
https://read.qxmd.com/read/38493928/efficacy-of-erythropoietin-as-a-neuroprotective-agent-in-ckd-associated-cognitive-dysfunction-a-literature-systematic-review
#14
REVIEW
Michelangela Barbieri, Paolo Chiodini, Piergiacomo Di Gennaro, Gaye Hafez, Sophie Liabeuf, Jolanta Malyszko, Laila-Yasmin Mani, Francesco Mattace Raso, Marion Pepin, Norberto Perico, Mariadelina Simeoni, Carmine Zoccali, Giovanni Tortorella, Annalisa Capuano, Giuseppe Remuzzi, Giovambattista Capasso, Giuseppe Paolisso
Patients with chronic kidney disease (CKD) often experience mild cognitive impairment and other neurocognitive disorders. Studies have shown that erythropoietin (EPO) and its receptor have neuroprotective effects in cell and animal models of nervous system disorders. Recombinant human EPO (rHuEPO), commonly used to treat anemia in CKD patients, could be a neuroprotective agent. In this systematic review, we aimed to assess the published studies investigating the cognitive benefits of rHuEPO treatment in individuals with reduced kidney function...
March 15, 2024: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://read.qxmd.com/read/38493085/drug-utilization-in-patients-starting-haemodialysis-with-a-focus-on-cardiovascular-and-antidiabetic-medications-an-epidemiological-study-in-the-lazio-region-italy-2016-2020
#15
JOURNAL ARTICLE
Ursula Kirchmayer, Claudia Marino, Sandro Feriozzi, Carlo Massimetti, Micol Manzuoli, Laura Angelici, Anna Maria Bargagli, Silvia Cascini, Antonio Addis, Marina Davoli, Nera Agabiti
BACKGROUND: Entering dialysis is a critical moment in patients' healthcare journey, and little is known about drug therapy around it. A study funded by the Italian Medicines Agency offered the opportunity to leverage data from the Lazio Regional Dialysis and Transplant Registry (RRDTL) and perform an observational study on drug use patterns before and after initiating chronic dialysis. METHODS: Individuals initiating dialysis in 2016-2020 were identified from RRDTL, excluding patients with prior renal transplantation, stopping dialysis early, or dying within 12 months...
March 16, 2024: BMC Nephrology
https://read.qxmd.com/read/38489143/clinical-effect-of-roxadustat-vs-erythropoietin-in-non-dialysis-ckd-with-diabetes-a-single-center-propensity-score-matching-analysis
#16
JOURNAL ARTICLE
Chen Jin, Yan Ren, Minmin Wang, Xiao Hu, Yiwei Shang, Yiwen Li, Bin Zhu, Qiang He, Lina Shao
PURPOSE: Renal anemia is a common complication of chronic kidney disease. Currently, recombinant human erythropoietin and roxadustat are the main treatments. In China, diabetic kidney disease is the primary cause of chronic kidney disease. However, high-quality evidence on the efficacy of roxadustat in patients with non-dialysis-dependent chronic kidney disease and diabetes mellitus is scarce. This study aimed to assess the clinical effect of roxadustat in such patients. METHODS: Patients with non-dialysis-dependent anemia and diabetes mellitus who received roxadustat or recombinant human erythropoietin for ≥ 4 weeks were enrolled...
March 15, 2024: International Urology and Nephrology
https://read.qxmd.com/read/38488446/erythropoietin-derived-peptide-ara290-mediates-brain-tissue-protection-through-the-%C3%AE-common-receptor-in-mice-with-cerebral-ischemic-stroke
#17
JOURNAL ARTICLE
Rong-Liang Wang, Zhen-Hong Yang, Yu-You Huang, Yue Hu, Yi-Lin Wang, Feng Yan, Yang-Min Zheng, Zi-Ping Han, Jun-Fen Fan, Zhen Tao, Hai-Ping Zhao, Si-Jie Li, Yu-Min Luo
AIM: To explore the neuroprotective effects of ARA290 and the role of β-common receptor (βCR) in a mouse model of middle cerebral artery occlusion (MCAO). METHODS: This study included male C57BL/6J mice that underwent MCAO and reperfusion. The neuroprotective effect of ARA290 on MCAO-induced brain injury was investigated using neurological function tests (Longa and modified neurological severity score). Cerebral infarction was examined by 2, 3, 5-triphenyl tetrazolium chloride staining, neuronal apoptosis was assessed by immunofluorescence staining, blood parameters were measured using a flow cytometry-based automated hematology analyzer, liquid chromatography with tandem mass spectrometry was used to identify the serum metabolomics signature, inflammatory cytokines and liver index were detected by commercially available kits, and the protein levels of the erythropoietin (EPO) receptor and βCR were measured by western blot...
March 2024: CNS Neuroscience & Therapeutics
https://read.qxmd.com/read/38488148/exploring-the-complex-impact-of-proteins-on-dopamine-polymerization-mechanisms-and-strategies-for-modulation
#18
JOURNAL ARTICLE
Chenxu Zhu, Huaisyuan Xie, Yihan Zhang, Ruilan Zhang, Shenbin Dai, Xinyi Li, Ying Sun, Yuxuan Zhang, Meiping Zhao
Polydopamine (pDA) is a valuable material with wide-ranging potential applications. However, the complex and debated nature of dopamine polymerization complicates our understanding. Specifically, the impact of foreign substances, especially proteins, on pDA formation adds an additional layer of subtlety and complexity. This study delves into specific surface features of proteins that predominantly shape their impact on dopamine polymerization. Notably, the biotin-binding site emerges as a critical region responsible for the pronounced inhibitory effect of avidin and neutravidin on the dopamine polymerization process...
March 15, 2024: Journal of Physical Chemistry. B
https://read.qxmd.com/read/38487970/influence-of-ionizable-lipid-tail-length-on-lipid-nanoparticle-delivery-of-mrna-of-varying-length
#19
JOURNAL ARTICLE
Kaitlin Mrksich, Marshall S Padilla, Ryann A Joseph, Emily L Han, Dongyoon Kim, Rohan Palanki, Junchao Xu, Michael J Mitchell
RNA-based therapeutics have gained traction for the prevention and treatment of a variety of diseases. However, their fragility and immunogenicity necessitate a drug carrier. Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing evidence that the structure of IL lipid tails significantly impacts the efficacy of LNP-mediated mRNA translation...
March 15, 2024: Journal of Biomedical Materials Research. Part A
https://read.qxmd.com/read/38482261/association-between-the-polymorphism-of-angiotensin-converting-enzyme-gene-and-interleukin-1-beta-gene-and-the-response-to-erythropoietin-therapy-in-dialysis-patients-with-anemia
#20
JOURNAL ARTICLE
P Dzekova-Vidimliski, N Eftimovska-Otovikj, I G Nikolov, Gj Selim, I Rambabova-Bushljetik, V Pushevski, V Karanfilovski, N Matevska-Geshovska, A Dimovski
INTRODUCTION: The polymorphism of the angiotensin-converting enzyme (ACE) gene and interleukin-1 beta (IL-1b) gene could be associated with resistance in the treatment of anemia in dialysis patients with recombinant human erythropoietin (rHuEPO). The aim of the study was to evaluate the association between the polymorphism of the ACE and IL-1b genes and the response to rHuEPO therapy in dialysis patients with anemia. MATERIAL AND METHODS: The study investigated 69 patients on dialysis with anemia treated with recombinant human erythropoietin for 12 months...
December 2023: Balkan Journal of Medical Genetics: BJMG
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