Hao Liu, Min Huang, Shuxin Wei, Xiaowen Wang, Yaqing Zhao, Zhengyang Han, Xianfeng Ye, Zhoukun Li, Yanling Ji, Zhongli Cui, Yan Huang
β-1,3-Galactanases selectively degrade β-1,3-galactan, thus it is an attractive enzyme technique to map high-galactan structure and prepare galactooligosaccharides. In this work, a gene encoding exo-β-1,3-galactanase (PxGal43) was screened form Paenibacillus xylanexedens, consisting of a GH43 domain, a CBM32 domain and α-L-arabinofuranosidase B (AbfB) domain. Using β-1,3-galactan (AG-II-P) as substrate, the recombined enzyme expressed in Escherichia coli BL21 (DE3) exhibited an optimal activity at pH 7...
April 4, 2024: International Journal of Biological Macromolecules