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https://www.readbyqxmd.com/read/28448640/mapping-of-the-lassa-virus-lamp1-binding-site-reveals-unique-determinants-not-shared-by-other-old-world-arenaviruses
#1
Hadar Israeli, Hadas Cohen-Dvashi, Anastasiya Shulman, Amir Shimon, Ron Diskin
Cell entry of many enveloped viruses occurs by engagement with cellular receptors, followed by internalization into endocytic compartments and pH-induced membrane fusion. A previously unnoticed step of receptor switching was found to be critical during cell entry of two devastating human pathogens: Ebola and Lassa viruses. Our recent studies revealed the functional role of receptor switching to LAMP1 for triggering membrane fusion by Lassa virus and showed the involvement of conserved histidines in this switching, suggesting that other viruses from this family may also switch to LAMP1...
April 27, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28445749/equilibrium-ensembles-for-insulin-folding-from-bias-exchange-metadynamics
#2
Richa Singh, Rohit Bansal, Anurag Singh Rathore, Gaurav Goel
Earliest events in the aggregation process, such as single molecule reconfiguration, are extremely important and the most difficult to characterize in experiments. To this end, we have used well-tempered bias exchange metadynamics simulations to determine the equilibrium ensembles of an insulin molecule under amyloidogenic conditions of low pH and high temperature. A bin-based clustering method that uses statistics accumulated in bias exchange metadynamics trajectories was employed to construct a detailed thermodynamic and kinetic model of insulin folding...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28445052/facile-preparation-of-drug-loaded-tristearin-encapsulated-superparamagnetic-iron-oxide-nanoparticles-using-coaxial-electrospray-processing
#3
Manoochehr Rasekh, Zeeshan Ahmad, Richard Barrie Michael Cross, Javier Hernández Gil, James D E T Wilton-Ely, Philip W Miller
Naturally occurring polymers are promising biocompatible materials that have many applications for emerging therapies, drug delivery systems and diagnostic agents. The handling and processing of such materials still constitutes a major challenge which can limit the full exploitation of their properties. This study explores an ambient environment processing technique: coaxial electrospray (CO-ES) to encapsulate genistein (an isoflavonoid and model drug), superparamagnetic iron oxide nanoparticles (SPIONs, 10-15 nm) and a fluorophore (BODIPY) into a layered (triglyceride tristearin shell) particulate system, with a view to constructing a theranostic agent...
April 26, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28443028/improved-model-of-proton-pump-crystal-structure-obtained-by-interactive-molecular-dynamics-flexible-fitting-expands-the-mechanistic-model-for-proton-translocation-in-p-type-atpases
#4
Dorota Focht, Tristan I Croll, Bjorn P Pedersen, Poul Nissen
The plasma membrane H(+)-ATPase is a proton pump of the P-type ATPase family and essential in plants and fungi. It extrudes protons to regulate pH and maintains a strong proton-motive force that energizes e.g., secondary uptake of nutrients. The only crystal structure of a H(+)-ATPase (AHA2 from Arabidopsis thaliana) was reported in 2007. Here, we present an improved atomic model of AHA2, obtained by a combination of model rebuilding through interactive molecular dynamics flexible fitting (iMDFF) and structural refinement based on the original data, but using up-to-date refinement methods...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28439081/long-lasting-neutralization-of-c5-by-sky59-a-novel-recycling-antibody-is-a-potential-therapy-for-complement-mediated-diseases
#5
Taku Fukuzawa, Zenjiro Sampei, Kenta Haraya, Yoshinao Ruike, Meiri Shida-Kawazoe, Yuichiro Shimizu, Siok Wan Gan, Machiko Irie, Yoshinori Tsuboi, Hitoshi Tai, Tetsushi Sakiyama, Akihisa Sakamoto, Shinya Ishii, Atsuhiko Maeda, Yuki Iwayanagi, Norihito Shibahara, Mitsuko Shibuya, Genki Nakamura, Takeru Nambu, Akira Hayasaka, Futa Mimoto, Yuu Okura, Yuji Hori, Kiyoshi Habu, Manabu Wada, Takaaki Miura, Tatsuhiko Tachibana, Kiyofumi Honda, Hiroyuki Tsunoda, Takehisa Kitazawa, Yoshiki Kawabe, Tomoyuki Igawa, Kunihiro Hattori, Junichi Nezu
Dysregulation of the complement system is linked to the pathogenesis of a variety of hematological disorders. Eculizumab, an anti-complement C5 monoclonal antibody, is the current standard of care for paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). However, because of high levels of C5 in plasma, eculizumab has to be administered biweekly by intravenous infusion. By applying recycling technology through pH-dependent binding to C5, we generated a novel humanized antibody against C5, SKY59, which has long-lasting neutralization of C5...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432970/insights-into-key-factors-controlling-go-stability-in-natural-surface-waters
#6
Yang Gao, Xuemei Ren, Xiaoli Tan, Tasawar Hayat, Ahmed Alsaedi, Changlun Chen
The effects of pH, cations (Na(+), K(+), Mg(2+), Ca(2+) and Al(3+)), and anions (Cl(-), HCO3(-), HPO4(2-) and SO4(2-)) on graphene oxide (GO) stability were investigated to address the current limitations in the knowledge regarding the stability of GO in natural surface water and its underlying mechanism. The threshold values of cations that destabilize GO were obtained and affected by both pH and anions. By employing elemental mapping and studying the effects of polyacrylic acid (PAA) on GO sedimentation and the re-dispersion of GO aggregates, we find that the GO aggregates induced by Na(+) and K(+) via electric double layer suppression and by Ca(2+) and Al(3+) via strong complxing are difficult to re-disperse completely...
April 11, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28427687/improved-adsorption-performance-of-nanostructured-composite-by-ultrasonic-wave-optimization-through-response-surface-methodology-isotherm-and-kinetic-studies
#7
Mehdi Dastkhoon, Mehrorang Ghaedi, Arash Asfaram, Alireza Goudarzi, Seyyedeh Maryam Mohammadi, Shaobin Wang
In this work, ultrasound-assisted adsorption of an anionic dye, sunset yellow (SY) and cationic dyes, malachite green (MG), methylene blue (MB) and their ternary dye solutions onto Cu@ Mn-ZnS-NPs-AC from water aqueous was optimized by response surface methodology (RSM) using the central composite design (CCD). The adsorbent was characterized using Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDX) and EDX mapping images. The effects of various parameters such as pH, sonication time, adsorbent mass and initial concentrations of SY, MG and MB were examined...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28411868/high-resolution-stream-water-quality-assessment-in-the-vancouver-british-columbia-region-a-citizen-science-study
#8
Scott M Shupe
Changing land cover and climate regimes modify water quantity and quality in natural stream systems. In regions undergoing rapid change, it is difficult to effectively monitor and quantify these impacts at local to regional scales. In Vancouver, British Columbia, one of the most rapidly urbanizing areas in Canada, 750 measurements were taken from a total of 81 unique sampling sites representing 49 streams located in urban, forest, and agricultural-dominant watersheds at a frequency of up to 12 times per year between 2013 and 2016...
April 12, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28404889/cryptic-bcr-abl-fusion-gene-as-variant-rearrangement-in-chronic-myeloid-leukemia-molecular-cytogenetic-characterization-and-influence-on-tkis-therapy
#9
Simona Luatti, Carmen Baldazzi, Giulia Marzocchi, Gaia Ameli, Maria Teresa Bochicchio, Simona Soverini, Fausto Castagnetti, Mario Tiribelli, Gabriele Gugliotta, Giovanni Martinelli, Michele Baccarani, Michele Cavo, Gianantonio Rosti, Nicoletta Testoni
At diagnosis, about 5% of Chronic Myeloid Leukemia (CML) patients lacks Philadelphia chromosome (Ph), despite the presence of the BCR/ABL rearrangement. Two mechanisms have been proposed about the occurrence of this rearrangement: the first one is a cryptic insertion between chromosomes 9 and 22; the second one involves two sequential translocations: a classic t(9;22) followed by a reverse translocation, which reconstitutes the normal morphology of the partner chromosomes. Out of 398 newly diagnosed CML patients, we selected 12 Ph-negative cases...
February 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28389720/superoxide-dismutase-1-is-positively-selected-to-minimize-protein-aggregation-in-great-apes
#10
Pouria Dasmeh, Kasper P Kepp
Positive (adaptive) selection has recently been implied in human superoxide dismutase 1 (SOD1), a highly abundant antioxidant protein with energy signaling and antiaging functions, one of very few examples of direct selection on a human protein product (exon); the molecular drivers of this selection are unknown. We mapped 30 extant SOD1 sequences to the recently established mammalian species tree and inferred ancestors, key substitutions, and signatures of selection during the protein's evolution. We detected elevated substitution rates leading to great apes (Hominidae) at ~1 per 2 million years, significantly higher than in other primates and rodents, although these paradoxically generally evolve much faster...
April 7, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28388846/three-dimensional-ph-mapping-within-model-hybrid-xerogel-thin-films
#11
Joel F Destino, Andrew K Craft, Frank V Bright
When xerogel films derived from carboxyethylsilanetriol (COE) and tetraethoxysilane (TEOS) or 3-aminopropyltriethoxysilane (APTES), n-octyltriethoxysilane (C8) and TEOS are formed on Al2O3 they exhibit chemically segregated domains with unique chemistries and topographies. These characteristics are important for marine antifouling. By using the ratiometric fluorescent probe 5-(and-6)-carboxy SNARF®-1 (C.SNARF-1) in concert with confocal fluorescence microscopy we determine the pH in three-dimensions within these hybrid films...
April 7, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28384183/qtl-mapping-of-wheat-plant-architectural-characteristics-and-their-genetic-relationship-with-seven-qtls-conferring-resistance-to-sheath-blight
#12
Yan Guo, Ziyi Du, Jiang Chen, Zhongjun Zhang
Sheath blight is one of the most devastating wheat diseases worldwide. Breeding resistant cultivars is the most powerful strategy to defeat the disease. Plant resistance on "disease escape" works through modulation of morphological traits and shows sustainable resistance to disease. Plant architectural traits have been reported to play a significant role in disease response. Therefore, exploring the genetic relationship between plant architecture and disease resistance is of importance to the understanding of plant resistance via "disease escape"...
2017: PloS One
https://www.readbyqxmd.com/read/28380601/genome-wide-association-study-in-an-f2-duroc-x-pietrain-resource-population-for-economically-important-meat-quality-and-carcass-traits
#13
S Casiró, D Velez-Irizarry, C W Ernst, N E Raney, R O Bates, M G Charles, J P Steibel
Meat quality is essential for consumer acceptance, it ultimately impacts pork production profitability and it is subject to genetic control. The objective of this study was to map genomic regions associated with economically important meat quality and carcass traits. We performed a genome-wide association (GWA) analysis to map regions associated with 38 meat quality and carcass traits recorded for 948 F2 pigs from the Michigan State University Duroc × Pietrain resource population. The F0, F1, and 336 F2 pigs were genotyped with the Illumina Porcine SNP60 BeadChip, while the remaining F2 pigs were genotyped with the GeneSeek Genomic Profiler for Porcine Low Desnisty (LD) chip, and imputed with high accuracy ( = 0...
February 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28378995/combinatorial-library-screening-with-liposomes-for-discovery-of-membrane-active-peptides
#14
Randy P Carney, Yann Thillier, Zsofia Kiss, Amir Sahabi, Jean Carlos Heleno Campos, Alisha Knudson, Ruiwu Liu, David Olivos, Mary Saunders, Lin Tian, Kit S Lam
Membrane active peptides (MAPs) represent a class of short biomolecules that have shown great promise in facilitating intracellular delivery without disrupting cellular plasma membranes. Yet their clinical application has been stalled by numerous factors: off-target delivery, a requirement for high local concentration near cells of interest, degradation en route to the target site, and, in the case of cell-penetrating peptides, eventual entrapment in endolysosomal compartments. The current method of deriving MAPs from naturally occurring proteins has restricted the discovery of new peptides that may overcome these limitations...
April 5, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28370530/noninvasive-evaluation-of-renal-ph-homeostasis-after-ischemia-reperfusion-injury-by-cest-mri
#15
Dario Livio Longo, Juan Carlos Cutrin, Filippo Michelotti, Pietro Irrera, Silvio Aime
Acute kidney injury (AKI) in mice caused by sustained ischemia followed by reperfusion is associated with acute tubular necrosis and renal dysfunctional blood flow. Although the principal role of the kidney is the maintenance of acid-base balance, current imaging approaches are unable to assess this important parameter, and clinical biomarkers are not robust enough in evaluating the severity of kidney damage. Therefore, novel noninvasive imaging approaches are needed to assess the acid-base homeostasis in vivo...
March 29, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28368631/strain-gradient-position-mapping-of-semiconductor-quantum-dots
#16
P-L de Assis, I Yeo, A Gloppe, H A Nguyen, D Tumanov, E Dupont-Ferrier, N S Malik, E Dupuy, J Claudon, J-M Gérard, A Auffèves, O Arcizet, M Richard, J-Ph Poizat
We introduce a nondestructive method to determine the position of randomly distributed semiconductor quantum dots (QDs) integrated in a solid photonic structure. By setting the structure in an oscillating motion, we generate a large stress gradient across the QDs plane. We then exploit the fact that the QDs emission frequency is highly sensitive to the local material stress to map the position of QDs deeply embedded in a photonic wire antenna with an accuracy ranging from ±35  nm down to ±1  nm. In the context of fast developing quantum technologies, this technique can be generalized to different photonic nanostructures embedding any stress-sensitive quantum emitters...
March 17, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28362742/single-wavelength-excited-photoacoustic-fluorescence-microscopy-for-in-vivo-ph-mapping
#17
Baoyun Yan, Huan Qin, Cuiyun Huang, Cong Li, Qun Chen, Da Xing
The balance between radiative and non-radiative decay is affected by various factors at the excitation state. A simultaneous acquisition of the fluorescence and photoacoustic signals from an appropriate probe provides an efficient, high-resolution means to monitor such a balance in a biological target, and thus may render its physiological information. Acidity plays an important role in tissue physiology. Here, we report an integrated photoacoustic-fluorescence microscopy (PA-FLM) for high-resolution (<3  μm) image mapping of interstitial pH by detecting the shift in the signal balance of a pH-sensitive probe...
April 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28355304/isolation-and-characterization-of-the-tasnrk2-10-gene-and-its-association-with-agronomic-traits-in-wheat-triticum-aestivum-l
#18
Zhao-Gui Zhang, Guang-de Lv, Bing Li, Jia-Jia Wang, Yan Zhao, Fan-Mei Kong, Ying Guo, Si-Shen Li
Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s...
2017: PloS One
https://www.readbyqxmd.com/read/28349699/limiting-the-hydrolysis-and-oxidation-of-maleimide-peptide-adducts-improves-detection-of-protein-thiol-oxidation
#19
Amber E Boyatzis, Scott D Bringans, Matthew J Piggott, Marisa N Duong, Richard J Lipscombe, Peter G Arthur
Oxidative stress, caused by reactive oxygen and nitrogen species (RONS), is important in the pathophysiology of many diseases. A key target of RONS is the thiol group of protein cysteine residues. As thiol oxidation can affect protein function, mechanistic information about how oxidative stress affects tissue function can be ascertained by identifying oxidized proteins. The probes used must be specific and sensitive, such as maleimides for the alkylation of reduced cysteine thiols. However, we find that maleimide-alkylated peptides (MAPs) are oxidized and hydrolysed under sample preparation conditions common for proteomic studies...
March 28, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28349175/characterization-of-the-temporal-and-spatial-expression-of-wheat-triticum-aestivum-l-plant-height-at-the-qtl-level-and-their-influence-on-yield-related-traits
#20
Na Zhang, Xiaoli Fan, Fa Cui, Chunhua Zhao, Wei Zhang, Xueqiang Zhao, Lijuan Yang, Ruiqing Pan, Mei Chen, Jie Han, Jun Ji, Dongcheng Liu, Zongwu Zhao, Yiping Tong, Aimin Zhang, Tao Wang, Junming Li
The temporal and spatial expression patterns of stable QTL for plant height and their influences on yield were characterized. Plant height (PH) is a complex trait in wheat (Triticum aestivum L.) that includes the spike length (SL) and the internode lengths from the first to the fifth internode, which are counted from the top and abbreviated as FIRITL, SECITL, THIITL, FOUITL, and FIFITL, respectively. This study identified eight putative additive quantitative trait loci (QTL) for PH. In addition, unconditional and conditional QTL mapping were used to analyze the temporal and spatial expression patterns of five stable QTL for PH...
March 27, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
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