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https://www.readbyqxmd.com/read/27916135/integrating-chemical-imaging-of-cationic-trace-metal-solutes-and-ph-into-a-single-hydrogel-layer
#1
Christoph Hoefer, Jakob Santner, Sergey M Borisov, Walter W Wenzel, Markus Puschenreiter
Gel-based, two-dimensional (2D) chemical imaging techniques are versatile methods for investigating biogeochemically active environments at high spatial resolution (sub-mm). State-of-the-art solute imaging techniques, such as diffusive gradients in thin films (DGT) and planar optodes (PO), employ passive solute sampling or sensing. Combining these methods will provide powerful tools for studying the biogeochemistry of biological niches in soils and sediments. In this study we aimed at developing a combined single-layer gel for direct pH imaging using PO and sampling of anionic and cationic solutes by DGT, with subsequent analysis of the bound solutes by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27914330/synergistic-effect-of-p-n-heterojunction-supporting-and-zeolite-nanoparticles-in-enhanced-photocatalytic-activity-of-nio-and-sno2
#2
Hadis Derikvandi, Alireza Nezamzadeh-Ejhieh
Increased photocatalytic activity of NiO and SnO2 was achieved by coupling and supporting of them onto clinoptilolite nanoparticles (NC) via calcination of Ni(II)-Sn(IV) - exchanged NC in photocatalytic degradation of metronidazole (MZ) aqueous solution. XRD, XRF, FTIR, SEM, X-ray mapping, DRS, TEM, BET Cyclic voltammetry and electrochemical impedance spectroscopy techniques (EIS) were used for characterization of samples. Red shift occurred in bang gap energies of the coupled semiconductors with respect to monocomponent one...
November 21, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27903630/genetic-causes-of-phenotypic-adaptation-to-the-second-fermentation-of-sparkling-wines-in-saccharomyces-cerevisiae
#3
Maria Martí-Raga, Emilien Peltier, Albert Mas, Gemma Beltran, Philippe Marullo
Hybridization is known to improve complex traits due to heterosis and phenotypic robustness. However, these phenomena have been rarely explained at the molecular level. Here, the genetic determinism of Saccharomyces cerevisiae fermentation performance was investigated using a QTL-mapping approach on an F1-progeny population. Three main QTLs were detected, with positive alleles coming from both parental strains. The heterosis effect found in the hybrid was partially explained by three loci showing pseudo-overdominance and dominance effect...
November 30, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27896628/clinical-translation-of-tumor-acidosis-measurements-with-acidocest-mri
#4
Kyle M Jones, Edward A Randtke, Eriko S Yoshimaru, Christine M Howison, Pavani Chalasani, Robert R Klein, Setsuko K Chambers, Phillip H Kuo, Mark D Pagel
PURPOSE: We optimized acido-chemical exchange saturation transfer (acidoCEST) magnetic resonance imaging (MRI), a method that measures extracellular pH (pHe), and translated this method to the radiology clinic to evaluate tumor acidosis. PROCEDURES: A CEST-FISP MRI protocol was used to image a flank SKOV3 tumor model. Bloch fitting modified to include the direct estimation of pH was developed to generate parametric maps of tumor pHe in the SKOV3 tumor model, a patient with high-grade invasive ductal carcinoma, and a patient with metastatic ovarian cancer...
November 28, 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/27869558/polynitroxylated-pegylated-hemoglobin-a-novel-small-volume-therapeutic-for-traumatic-brain-injury-resuscitation-comparison-to-whole-blood-and-dose-response-evaluation
#5
Erik C Brockman, Travis C Jackson, C Edward Dixon, Hulya Bayir, Robert Clark, Vincent A Vagni, Keri L Janesko, Catherine Byrd, Li Ma, Carleton J C Hsia, Patrick M Kochanek
: Resuscitation with polynitroxylated pegylated hemoglobin (PNPH), a PEGylated bovine hemoglobin decorated with nitroxides, eliminated the need for fluid administration, reduced ICP and brain edema, and produced neuroprotection in vitro and in vivo vs. lactated Ringer's (LR) in experimental traumatic brain injury (TBI) plus hemorrhagic shock (HS). HYPOTHESIS: Resuscitation with PNPH will improve acute physiology vs. whole blood after TBI+HS and will be safe and effective across a wide dosage range...
November 21, 2016: Journal of Neurotrauma
https://www.readbyqxmd.com/read/27862510/dynamic-31-p-mrsi-using-spiral-spectroscopic-imaging-can-map-mitochondrial-capacity-in-muscles-of-the-human-calf-during-plantar-flexion-exercise-at-7%C3%A2-t
#6
Ladislav Valkovič, Marek Chmelík, Martin Meyerspeer, Borjan Gagoski, Christopher T Rodgers, Martin Krššák, Ovidiu C Andronesi, Siegfried Trattnig, Wolfgang Bogner
Phosphorus MRSI ((31) P-MRSI) using a spiral-trajectory readout at 7 T was developed for high temporal resolution mapping of the mitochondrial capacity of exercising human skeletal muscle. The sensitivity and localization accuracy of the method was investigated in phantoms. In vivo performance was assessed in 12 volunteers, who performed a plantar flexion exercise inside a whole-body 7 T MR scanner using an MR-compatible ergometer and a surface coil. In five volunteers the knee was flexed (~60°) to shift the major workload from the gastrocnemii to the soleus muscle...
December 2016: NMR in Biomedicine
https://www.readbyqxmd.com/read/27853705/shelf-life-evaluation-of-ricotta-fresca-sheep-cheese-in-modified-atmosphere-packaging
#7
Carlo Pala, Christian Scarano, Massimiliano Venusti, Daniela Sardo, Daniele Casti, Francesca Cossu, Sonia Lamon, Vincenzo Spanu, Michela Ibba, Michela Marras, Antonio Paba, Carlo Spanu, Enrico Pietro Luigi De Santis
Ricotta fresca cheese is the product of Sardinian dairy industry most exposed to microbial post-process contamination. Due to its technological characteristics, intrinsic parameters, pH (6.10-6.80) and water activity (0.974-0.991), it represents an excellent substrate for the growth of spoilage and pathogenic microorganisms, which are usually resident in cheese-making plants environments. Generally, ricotta fresca has a shelf life of 5-7 days. For this reason, at industrial level, modified atmosphere packaging (MAP) is used to extend the durability of the product...
June 3, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/27825028/peripartum-hysterectomy-incidence-risk-factors-and-clinical-characteristics-in-ireland
#8
Sarah M Campbell, Paul Corcoran, Edel Manning, Richard A Greene
BACKGROUND: The incidence of peripartum hysterectomy (PH) shows fifty-fold variation worldwide (0.2-10.5/1000 deliveries) and risk factors include advancing maternal age and parity, previous caesarean section (CS) and abnormal placentation. OBJECTIVES: In this first national study of PH in Ireland, our objectives were threefold: to describe the national trend in PH incidence over 15 years since 1999; to assess risk of PH associated with morbidly adherent placenta (MAP), placenta praevia and postpartum haemorrhage (PPH) during 2005-2013; and to describe the causes, interventions and outcomes of PH cases during 2011-2013...
October 27, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27822741/isolated-brain-trauma-in-cats-triggers-rapid-onset-of-hypovolemia
#9
Peter Bentzer, Per-Olof Grände
BACKGROUND: Hemodynamic instability responsive to fluid resuscitation is common after a traumatic brain injury (TBI), also in the absence of systemic hemorrhage. The present study tests if an isolated severe TBI induces a decrease in plasma volume (PV). METHODS: The study was performed in three groups of anesthetized and tracheostomized male cats (n = 21). In one group (n = 8), the cats were prepared with a cranial borehole (10 mm i.d) used to expose the brain to a fluid percussion brain injury (FPI) (1...
November 7, 2016: Neurocritical Care
https://www.readbyqxmd.com/read/27806589/real-time-4d-dose-reconstruction-for-tracked-dynamic-mlc-deliveries-for-lung-sbrt
#10
Cornelis Ph Kamerling, Martin F Fast, Peter Ziegenhein, Martin J Menten, Simeon Nill, Uwe Oelfke
PURPOSE: This study provides a proof of concept for real-time 4D dose reconstruction for lung stereotactic body radiation therapy (SBRT) with multileaf collimator (MLC) tracking and assesses the impact of tumor tracking on the size of target margins. METHODS: The authors have implemented real-time 4D dose reconstruction by connecting their tracking and delivery software to an Agility MLC at an Elekta Synergy linac and to their in-house treatment planning software (TPS)...
November 2016: Medical Physics
https://www.readbyqxmd.com/read/27805910/nanoring-formation-via-in-situ-photoreduction-of-silver-on-a-virus-scaffold
#11
Serene S Bayram, Omar K Zahr, Julia Del Re, Amy Szuchmacher Blum
The fabrication of plasmonic nanorings remains of substantial interest by virtue of their enhanced electric and magnetic response to light fields which can be subsequently exploited in diverse applications. Scaling down the size of nanorings holds promise in creating artificial magnetism at wavelengths matching the solar spectrum. Nanosized bioscaffolds can be utilized to tackle the challenge of size reduction of metallic rings owing to their miniature features as well as their well-known biomineralization capacity...
November 2, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27799424/in-situ-raman-mapping-of-art-objects
#12
D Lauwers, Ph Brondeel, L Moens, P Vandenabeele
Raman spectroscopy has grown to be one of the techniques of interest for the investigation of art objects. The approach has several advantageous properties, and the non-destructive character of the technique allowed it to be used for in situ investigations. However, compared with laboratory approaches, it would be useful to take advantage of the small spectral footprint of the technique, and use Raman spectroscopy to study the spatial distribution of different compounds. In this work, an in situ Raman mapping system is developed to be able to relate chemical information with its spatial distribution...
December 13, 2016: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/27795693/inheritance-and-qtl-analysis-of-the-determinants-of-flower-color-in-tetraploid-cut-roses
#13
Virginia W Gitonga, Robert Stolker, Carole F S Koning-Boucoiran, Mitra Aelaei, Richard G F Visser, Chris Maliepaard, Frans A Krens
The success of cut rose cultivars is a direct result of their aesthetic value. The rose industry thrives on novelty, and the production of novel flower color has been extensively studied. The most popular color is red, and it is, therefore, important for breeders to produce a good red cultivar. The final visible color of the flower is a combination of a number of factors including the type of anthocyanin accumulating, modifications to the anthocyanidin molecule, co-pigmentation and vacuolar pH. Here, we analyze the quantitative variation of the biochemical constituents of flower color in a tetraploid rose population and combine this with marker information in the segregating rose population to map the chromosomal locations of putative QTLs for flower color traits...
2016: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/27782169/valproate-inhibits-map-kinase-signalling-and-cell-cycle-progression-in-s-cerevisiae
#14
Kristelle Desfossés-Baron, Ian Hammond-Martel, Antoine Simoneau, Adnane Sellam, Stephen Roberts, Hugo Wurtele
The mechanism of action of valproate (VPA), a widely prescribed short chain fatty acid with anticonvulsant and anticancer properties, remains poorly understood. Here, the yeast Saccharomyces cerevisiae was used as model to investigate the biological consequences of VPA exposure. We found that low pH strongly potentiates VPA-induced growth inhibition. Transcriptional profiling revealed that under these conditions, VPA modulates the expression of genes involved in diverse cellular processes including protein folding, cell wall organisation, sexual reproduction, and cell cycle progression...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27780075/thermal-intensification-of-microbial-fe-ii-fe-iii-redox-cycling-in-a-pristine-shallow-sand-aquifer-on-the-canadian-shield
#15
V L Shirokova, A M L Enright, C B Kennedy, F G Ferris
This investigation evaluates spatial relationships between summer (July) groundwater temperatures and Fe(II)/Fe(III) biogeochemical cycling over a five year period in a shallow pristine sand aquifer at Meilleurs Bay near Deep River, Ontario, Canada. A warm subsurface thermal island of 12.5-16.1 °C, compared to background conditions of 10-11 °C, was manifest in contour maps of average groundwater temperature over the study period. The warm zone coincided with an area of convergent groundwater flow, implicating horizontal heat transfer by advective convection as the reason for elevated temperatures...
October 19, 2016: Water Research
https://www.readbyqxmd.com/read/27779578/does-small-volume-resuscitation-with-crystalloids-or-colloids-influence-hemostasis-and-survival-of-rabbits-subjected-to-lethal-uncontrolled-hemorrhage
#16
Bijan S Kheirabadi, Nahir Miranda, Irasema B Terrazas, Mary D Gonzales, Rose C Grimm, Michael A Dubick
INTRODUCTION: Prehospital, small-volume resuscitation of combat casualties with a synthetic colloid [6% hydroxyethyl starch (HES) 670/0.75] has been recommended when blood or blood components are unavailable. We studied hemostatic effects of a newer synthetic colloid (6% HES 130/0.4,) compared to either a natural colloid (albumin) or to crystalloids in an uncontrolled hemorrhage model. METHODS: Spontaneously breathing NZW rabbits (3.4±0.1 kg) were anesthetized, instrumented and subjected to a splenic injury with uncontrolled bleeding...
October 25, 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/27768832/articular-cartilage-injury-rapidly-activates-tak1-in-chondrocytes-by-inducing-its-phosphorylation-and-k63-polyubiquitination
#17
Heba M Ismail, Athanasios Didangelos, Tonia L Vincent, Jeremy Saklatvala
Objectives Mechanical injury to cartilage predisposes to osteoarthritis (OA). Wounding the articular cartilage surface causes rapid activation of MAP kinases and NFκB mimicking the response to inflammatory cytokines. Here, we identify the upstream signalling mechanisms involved. Methods Cartilage was injured by dissecting it from the articular surface of porcine metacarpophalangeal joints (MCP) or by avulsing murine proximal femoral epiphyses. Protein phosphorylation was assayed by Western blotting of cartilage lysates...
October 21, 2016: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/27766103/development-of-prediction-model-and-experimental-validation-in-predicting-the-curcumin-content-of-turmeric-curcuma-longa-l
#18
Abdul Akbar, Ananya Kuanar, Raj K Joshi, I S Sandeep, Sujata Mohanty, Pradeep K Naik, Antaryami Mishra, Sanghamitra Nayak
The drug yielding potential of turmeric (Curcuma longa L.) is largely due to the presence of phyto-constituent 'curcumin.' Curcumin has been found to possess a myriad of therapeutic activities ranging from anti-inflammatory to neuroprotective. Lack of requisite high curcumin containing genotypes and variation in the curcumin content of turmeric at different agro climatic regions are the major stumbling blocks in commercial production of turmeric. Curcumin content of turmeric is greatly influenced by environmental factors...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27761530/spatial-assessment-of-soil-nitrogen-availability-and-varying-effects-of-related-main-soil-factors-on-soil-available-nitrogen
#19
Mingkai Qu, Weidong Li, Chuanrong Zhang, Biao Huang, Yongcun Zhao
To effectively understand the availability of soil nitrogen and assist in soil nitrogen control at the regional scale, it is essential to understand the accurate spatial distribution patterns of the three soil nitrogen parameters [i.e., total nitrogen (TN), available nitrogen (AN) and nitrogen availability ratio (NAR)] and explore the spatially varying influences of major impact factors on soil AN. Land use affects the spatial distributions of soil TN, AN and NAR (i.e., AN/TN). To explore the effects of different land use types and improve mapping accuracy, residual kriging with land use information and ordinary kriging (without land use information) were compared based on the sample data of soil TN and AN in Hanchuan county, China...
October 20, 2016: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/27743134/environmental-assessment-of-potential-toxic-trace-element-contents-in-the-inundated-floodplain-area-of-tablas-de-daimiel-wetland-spain
#20
R Jiménez-Ballesta, F J García-Navarro, S Bravo, J A Amorós, C Pérez-de-Los-Reyes, M Mejías
Contamination of aquatic systems with potentially toxic trace elements (PTEs) is a major problem throughout the world. The National Park Tablas de Daimiel (NPTD) is considered to make up one of the two most important wetlands in the Biosphere Reserve called "Wet Spot." Since PTEs are good indicator of the prevailing environmental conditions and possible contamination, soil samples collected from 43 sites were analyzed in order to investigate the levels and its distribution of these elements, in the inundated floodplain area of the NPTD wetland...
October 14, 2016: Environmental Geochemistry and Health
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