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https://www.readbyqxmd.com/read/28523639/association-mapping-of-yield-and-yield-related-traits-under-reproductive-stage-drought-stress-in-rice-oryza-sativa-l
#1
B P Mallikarjuna Swamy, Noraziyah Abd Aziz Shamsudin, Site Noorzuraini Abd Rahman, Ramil Mauleon, Wickneswari Ratnam, Ma Teressa Sta Cruz, Arvind Kumar
BACKGROUND: The identification and introgression of major-effect QTLs for grain yield under drought are some of the best and well-proven approaches for improving the drought tolerance of rice varieties. In the present study, we characterized Malaysian rice germplasm for yield and yield-related traits and identified significant trait marker associations by structured association mapping. RESULTS: The drought screening was successful in screening germplasm with a yield reduction of up to 60% and heritability for grain yield under drought was up to 78%...
December 2017: Rice
https://www.readbyqxmd.com/read/28521709/modulation-of-inflammatory-response-to-implanted-biomaterials-using-natural-compounds
#2
Maria Yanez, James Blanchette, Ehsan Jabbarzadeh
Tissue engineering offers a promising strategy to restore injuries resulting from trauma, infection, tumor resection, or other diseases. In spite of significant progress, the field faces a significant bottleneck; the critical need to understand and exploit the interdependencies of tissue healing, angiogenesis, and inflammation. Inherently, the balance of these interacting processes is affected by a number of injury site conditions that represent a departure from physiological environment, including reduced pH, increased concentration of free radicals, hypoglycemia, and hypoxia...
May 10, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28520947/synergistic-effect-of-spice-extracts-and-modified-atmospheric-packaging-towards-non-thermal-preservation-of-chicken-meat-under-refrigerated-storage
#3
M Sivarajan, U Lalithapriya, Peter Mariajenita, B Aafrin Vajiha, K Harini, D Madhushalini, M Sukumar
This study investigates the integrated approach of spice extracts and modified atmospheric packaging (MAP) chicken meat preservation. Specifically, extracts from clove (CL), cinnamon (CI) individually and in combination (3% w/w) along with MAP (30% CO2/70% N2 and 10% O2/30% CO2/60% N2) were used to increase the shelf life of fresh chicken meat stored at 4°C. The parameters evaluated as shelf life indications are microbiological (total viable count, Pseudomonas spp., lactic acid bacteria (LAB), and Enterobacteriaceae), physicochemical (pH, Lipid oxidation, color changes) and Sensory attributes...
May 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28516764/an-autoinhibitory-role-for-the-pleckstrin-homology-domain-of-itk-and-its-interplay-with-canonical-phospholipid-recognition
#4
Sujan Devkota, Raji E Joseph, Scott E Boyken, D Bruce Fulton, Amy H Andreotti
Pleckstrin homology (PH) domains are well known as phospholipid binding modules yet evidence is mounting that PH domain function extends beyond lipid recognition. In the current work, we characterize a protein binding function for the PH domain of Interleukin-2-inducible tyrosine kinase (ITK), an immune cell specific signaling protein that belongs to the TEC family of non-receptor tyrosine kinases. Its N-terminal Pleckstrin homology (PH) domain is a well characterized lipid-binding module that localizes ITK to the membrane via phosphatidylinositol (3,4,5)-trisphosphate (PIP3) binding...
May 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28516510/proteomic-analysis-of-lupinus-angustifolius-var-zeus-and-bojar-and-lupinus-luteus-var-lord-and-parys-seed-proteins-and-their-hydrolysates
#5
Jaroslaw Czubinski, Magdalena Montowska, Edward Pospiech, Eleonora Lampart-Szczapa
BACKGROUND: Proteins enzymatic digestion is a very complex process, during which some components are degraded, while others remain in an unchanged form. Moreover, the enzymatic hydrolysis is one of the most popular methods used to reduce the allergenicity of food proteins. In this study, the efficiency of enzymatic hydrolysis of lupin seeds proteins was assessed by proteomic analysis performed by two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry identification...
May 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28514937/modelling-and-optimization-of-a-nanophotocatalytic-process-using-fe-ag-zno-under-visible-led-irradiation-for-dibutyl-phthalate-removal-from-aqueous-environments
#6
B Akbari-Adergani, M H Saghi, A Eslami, A Mohseni-Bandpei, M Rabbani
An (Fe, Ag) co-doped ZnO nanostructure was synthesized by a simple chemical co-precipitation method and used for the degradation of dibutyl phthalate (DBP) in aqueous solution under visible LED irradiation. (Fe, Ag) co-doped ZnO nanorods were characterized by powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), UV-Vis DRS, elemental mapping, Field emission scanning electron microscopy (FE-SEM), TEM and Brunauer-Emmett-Teller (BET) surface area analysis. A Central Composite Design was used to optimize the reaction parameters for the removal of DBP by the (Fe, Ag) co-doped ZnO nanorods...
May 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28513002/one-oxygen-breath-shortened-the-time-to-return-of-spontaneous-circulation-in-severely-asphyxiated-piglets
#7
Rikard Linner, Doris Cunha-Goncalves, Valeria Perez-de-Sa
AIM: Asphyxiated neonates should be resuscitated with air, but it remains unclear if oxygen supplementation is needed in ineffectively ventilated newborn infants. We studied the return of spontaneous circulation (ROSC) with oxygen or air in an experimental model of inadequate ventilation. METHODS: Asphyxia was induced in 16 newborn piglets until their heart rate was <60 bpm or mean arterial pressure (MAP) <30 mmHg. During the first 10 minutes of resuscitation, they received one breath per minute of oxygen (n=8) or air (n=8)...
May 16, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28508362/quantitative-phosphoproteomic-analysis-of-brain-tissues
#8
Bing Bai, Haiyan Tan, Junmin Peng
Protein phosphorylation regulates brain development and neuronal activities; and dysregulation of phosphorylation contributes to neurobiological disorders. Phosphoproteomic analysis provides comprehensive modification maps for measuring protein activities in cellular pathways and biological processes. Here, we introduce a mass spectrometry (MS)-based protocol to quantitatively analyze the phosphoproteome of human postmortem brains of Alzheimer's disease. In this isobaric labeling protocol, up to ten brain samples are selected from control and diseased cases for comparison...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28507682/sabre-hyperpolarisation-of-vitamin-b3-as-a-function-of-ph
#9
A M Olaru, M J Burns, G G R Green, S B Duckett
In this work we describe how the signal enhancements obtained through the SABRE process in methanol-d4 solution are significantly affected by pH. Nicotinic acid (vitamin B3, NA) is used as the agent, and changing pH is shown to modify the level of polarisation transfer by over an order of magnitude, with significant improvements being seen in terms of the signal amplitude and relaxation rate at high pH values. These observations reveal that manipulating pH to improve SABRE enhancements levels may improve the potential of this method to quantify low concentrations of analytes in mixtures...
March 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28503732/imaging-short-lived-reactive-oxygen-species-ros-with-endogenous-contrast-mri
#10
Rong-Wen Tain, Alessandro M Scotti, Weiguo Li, Xiaohong Joe Zhou, Kejia Cai
PURPOSE: To characterize the relaxation properties of reactive oxygen species (ROS) for the development of endogenous ROS contrast magnetic resonance imaging (MRI). MATERIALS AND METHODS: ROS-producing phantoms and animal models were imaged at 9.4T MRI to obtain T1 and T2 maps. Egg white samples treated with varied concentrations of hydrogen peroxide (H2 O2 ) were used to evaluate the effect of produced ROS in T1 and T2 for up to 4 hours. pH and temperature changes due to H2 O2 treatment in egg white were also monitored...
May 15, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28501855/extracellular-ph-is-a-biomarker-enabling-detection-of-breast-cancer-and-liver-cancer-using-cest-mri
#11
Miaomiao Chen, Chaoying Chen, Zhiwei Shen, Xiaolei Zhang, Yanzi Chen, Fengfeng Lin, Xilun Ma, Caiyu Zhuang, Yifei Mao, Haochuan Gan, Peidong Chen, Xiaodan Zong, Renhua Wu
Extracellular pH (pHe) decrease is associated with tumor growth, invasion, metastasis, and chemoresistance, which can be detected by chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI). Here, we demonstrated that ioversol CEST MRI can be exploited to achieve pHe mapping of the liver cancer microenvironment. In in vitro studies, we firstly explored whether ioversol signal is pH-dependent, and calculated the function equation between the CEST effects of ioversol and pH values, in the range of 6...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28492229/imaging-of-ph-in-vivo-using-hyperpolarized-13-c-labelled-zymonic-acid
#12
Stephan Düwel, Christian Hundshammer, Malte Gersch, Benedikt Feuerecker, Katja Steiger, Achim Buck, Axel Walch, Axel Haase, Steffen J Glaser, Markus Schwaiger, Franz Schilling
Natural pH regulatory mechanisms can be overruled during several pathologies such as cancer, inflammation and ischaemia, leading to local pH changes in the human body. Here we demonstrate that (13)C-labelled zymonic acid (ZA) can be used as hyperpolarized magnetic resonance pH imaging sensor. ZA is synthesized from [1-(13)C]pyruvic acid and its (13)C resonance frequencies shift up to 3.0 p.p.m. per pH unit in the physiological pH range. The long lifetime of the hyperpolarized signal enhancement enables monitoring of pH, independent of concentration, temperature, ionic strength and protein concentration...
May 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/28489889/an-environmental-assessment-and-risk-map-of-ascaris-lumbricoides-and-necator-americanus-distributions-in-manufahi-district-timor-leste
#13
Rebecca Wardell, Archie C A Clements, Aparna Lal, David Summers, Stacey Llewellyn, Suzy J Campbell, James McCarthy, Darren J Gray, Susana V Nery
BACKGROUND: In Timor-Leste there have been intermittent and ineffective soil-transmitted helminth (STH) deworming programs since 2004. In a resource-constrained setting, having information on the geographic distribution of STH can aid in prioritising high risk communities for intervention. This study aimed to quantify the environmental risk factors for STH infection and to produce a risk map of STH in Manufahi district, Timor-Leste. METHODOLOGY/PRINCIPAL FINDINGS: Georeferenced cross-sectional data and stool samples were obtained from 2,194 participants in 606 households in 24 villages in the Manufahi District as part of cross sectional surveys done in the context of the "WASH for Worms" randomised controlled trial...
May 10, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28484481/qtl-mapping-for-phosphorus-efficiency-and-morphological-traits-at-seedling-and-maturity-stages-in-wheat
#14
Yuanyuan Yuan, Minggang Gao, Mingxia Zhang, Honghua Zheng, Xiuwen Zhou, Ying Guo, Yan Zhao, Fangmei Kong, Sishen Li
Phosphorus (P) efficiency (PE), which comprises phosphorus uptake (PupE) and utilization efficiency (PutE), is considered as one of the most important factors for crop yield. In the present study, 11 seedling traits and 13 maturity traits related to wheat PE and morphology were investigated using a set of recombinant inbred lines (RILs) derived from the cross of "TN 18 × LM 6," under hydroponic culture trials and field trials at low P (LP) and normal P (NP) levels in two different years, respectively. The LP input reduced of biomass, yield and PupE traits, but increased PutE traits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28475345/mechanical-properties-of-membranes-composed-of-gel-phase-or-fluid-phase-phospholipids-probed-on-liposomes-by-atomic-force-spectroscopy
#15
Oumaima Et-Thakafy, Nicolas Delorme, Cedric Gaillard, Cristelle Mériadec, Franck Artzner, Christelle Lopez, Fanny Guyomarc'h
In many liposome applications, the nanomechanical properties of the membrane envelope are essential to ensure e.g. physical stability, protection or penetration into tissues. Of all factors, the lipid composition and its phase behavior are susceptible to tune the mechanical properties of membranes. To investigate this, small unilamellar vesicles (SUV; diameter <200 nm), referred to as liposomes, were produced using either the unsaturated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or the saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in aqueous buffer at pH 6...
May 5, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28475300/facile-synthesis-of-three-dimensional-mg-al-layered-double-hydroxide-partially-reduced-graphene-oxide-nanocomposites-for-the-effective-removal-of-pb-2-from-aqueous-solution
#16
G Bishwa Bidita Varadwaj, Oluwaseun A Oyetade, Surjyakanta Rana, Bice S Martincigh, Sreekantha B Jonnalagadda, Vincent O Nyamori
A series of three-dimensional (3D) porous nanocomposites, comprised of partially reduced graphene oxide (pRGO) and CO3(2-) containing Mg-Al layered double hydroxide, were synthesized in two steps. In the first step, graphene oxide (GO) was fabricated by a modified Hummers' method, and, subsequently, in the second step layered double hydroxide (LDH) nanosheets were homogeneously grown on the surface of the GO sheets by an in situ crystallization approach, involving a facile coprecipitation technique. The alkaline medium used for the in situ growth of LDH on the GO surface resulted in the partial reduction of GO to pRGO, which was confirmed by XRD...
May 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28467057/conditional-chaperone-client-interactions-revealed-by-genetically-encoded-photo-cross-linkers
#17
Shuai Zhang, Dan He, Zhi Lin, Yi Yang, Haiping Song, Peng R Chen
The cell envelope is an integral and essential component of Gram-negative bacteria. As the front line during host-pathogen interactions, it is directly challenged by host immune responses as well as other harsh extracellular stimuli. The high permeability of the outer-membrane and the lack of ATP energy system render it difficult to maintain important biological activities within the periplasmic space under stress conditions. The HdeA/B chaperone machinery is the only known acid resistant system found in bacterial periplasm, enabling enteric pathogens to survive through the highly acidic human stomach and establish infections in the intestine...
May 3, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28464235/multi-criteria-manufacturability-indices-for-ranking-high-concentration-monoclonal-antibody-formulations
#18
Yang Yang, Ajoy Velayudhan, Nina F Thornhill, Suzanne S Farid
The need for high-concentration formulations for subcutaneous delivery of therapeutic monoclonal antibodies (mAbs) can present manufacturability challenges for the final ultrafiltration/diafiltration (UF/DF) step. Viscosity levels and the propensity to aggregate are key considerations for high-concentration formulations. This work presents novel frameworks for deriving a set of manufacturability indices related to viscosity and thermostability to rank high-concentration mAb formulation conditions in terms of their ease of manufacture...
May 2, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28462206/formulation-and-shelf-life-of-fish-burgers-served-to-preschool-children
#19
Giorgio Smaldone, Raffaele Marrone, Tiziana Zottola, Lucia Vollano, Giulio Grossi, Maria Luisa Cortesi
Consumer is very careful about healthiness; in this context nutritionists often highlight the importance of fish for human nutrition because of their protein and fatty acid composition. In order to stimulate utilisation and consumption of fish species by unusual target groups such as children, the aim of this research was to formulate and to evaluate shelf-life and nutritional values of fish preparations stored in modified atmosphere packaging (MAP). Fish species used for trail were Trachurus trachurus and Oncorhynchus mykiss fished and farmed in Basilicata region respectively...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28459798/resuscitative-endovascular-balloon-occlusion-of-the-aorta-reboa-for-major-abdominal-venous-injury-in-a-porcine-hemorrhagic-shock-model
#20
Michael S Lallemand, Donald M Moe, John M McClellan, Joshua P Smith, Leo Daab, Shannon Marko, Nam Tran, Benjamin Starnes, Matthew J Martin
BACKGROUND: Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) is a rescue maneuver for unstable patients with non-compressible hemorrhage below the diaphragm. The efficacy of REBOA in the setting a major abdominal venous injury is unknown. Our objective was to examine the use of REBOA in a large animal model of major abdominal venous injury, and characterize any impact on the hemodynamics, rate and volume of hemorrhage, and survival. METHODS: Ten swine (35-55kg) underwent a controlled and validated hemorrhage and ischemia/reperfusion injury protocol to produce shock physiology...
April 28, 2017: Journal of Trauma and Acute Care Surgery
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