Read by QxMD icon Read

pH mapping

Subhodeep Sarker, Deepti Vashistha, Munmun Saha Sarker, A Sarkar
Molecular biomarkers are used world wide for quick assessment of the immediate effect of environmental pollution on marine ecosystems. Recently, we evaluated oxidative stress responses of marine rock oyster, Saccostrea cucullata impacted due to polycyclic aromatic hydrocarbons (PAHs) accumulated in their tissues at a few sampling sites along the coast of Goa around the region of the Arabian sea coast, India (Sarkar et al., 2017). Using a combination of partial alkaline unwinding and comet assays, we now report a comprehensive study on the impairment of DNA integrity (DI) in S...
January 11, 2018: Ecotoxicology and Environmental Safety
Zecong Ding, Fenglian Fu, Dionysios D Dionysiou, Bing Tang
Naturally occurring ferrihydrite often contains various impurities, and Al is one of the most prominent impurities. However, little is known about how these impurities impact the physical and chemical properties of ferrihydrite with respect to metal(loid) adsorption. In this study, a series of Al-containing ferrihydrites were synthesized and exposed to a mixed solution containing As(III) and Cr(VI). The results showed that the two contaminants can be quickly adsorbed onto the surface of Al-containing ferrihydrite under acidic and neutral conditions...
January 11, 2018: Environmental Pollution
Rachael M Kenney, Matthew W Boyce, Nathan Whitman, Brenden P Kromhout, Matthew R Lockett
Paper-based cultures are an emerging platform for preparing 3D tissue-like structures. Chemical gradients can be imposed upon these cultures, generating microenvironments similar to those found in poorly vascularized tumors. There is increasing evidence that the tumor microenvironment is responsible for promoting drug resistance and increased invasiveness. Acidosis, or the acidification of the extracellular space, is particularly important in promoting these aggressive cancer phenotypes. To better understand how cells respond to acidosis there is a need for 3D culture platforms that not only model relevant disease states, but also contain sensors capable of quantifying the extracellular environment...
January 11, 2018: Analytical Chemistry
J Sánchez-España, K Wang, C Falagán, I Yusta, W D Burgos
Through the use of scanning transmission electron microscopy (STEM) combined with other complementary techniques (SEM, cryo-TEM, HRTEM, and EELS), we have studied the interaction of microorganisms inhabiting deep anoxic waters of acidic pit lakes with dissolved aluminum, silica, sulfate, and ferrous iron. These elements were close to saturation (Al, SiO2 ) or present at very high concentrations (0.12 m Fe(II), 0.12-0.22 m SO42- ) in the studied systems. The anaerobic conditions of these environments allowed investigation of geomicrobial interactions that are difficult to see in oxidized, Fe(III)-rich environments...
January 2018: Geobiology
Han-Sheng Liang, Yi Feng
OBJECTIVE: To observe the effect of transcutaneous acupoint electrical stimulation (TAES) on hemodynamic fluctuation caused by loosing tourniquet in the elderly patients undergoing knee joint replacement. METHODS: A total of 60 ASA (America Society Anesthesiologist) I or II elderly patients for elective knee joint replacement surgery were randomly divided into control group (30 cases) and TAES group (30 cases). Patients of both groups were treated by intravenous anesthesia, and monitored with bispectral index (BIS, between 45-60) for anesthesia depth, stroke volume variation (SVV) for fluid management, mean arterial pressure (MAP) and cardiac index (CI) for hemodynamic fluctuation evaluation, and with analgesia nociception index (ANI, between 50-70) for remifentanil dosage adjustment...
December 25, 2017: Zhen Ci Yan Jiu, Acupuncture Research
Casey P Johnson, Gary E Christensen, Jess G Fiedorowicz, Merry Mani, Joseph J Shaffer, Vincent A Magnotta, John A Wemmie
OBJECTIVES: Quantitative mapping of T1 relaxation in the rotating frame (T1ρ) is a magnetic resonance imaging technique sensitive to pH and other cellular and microstructural factors, and is a potentially valuable tool for identifying brain alterations in bipolar disorder. Recently, this technique identified differences in the cerebellum and cerebral white matter of euthymic patients vs healthy controls that were consistent with reduced pH in these regions, suggesting an underlying metabolic abnormality...
January 7, 2018: Bipolar Disorders
Xuan Zhou, Lian-Jin Jin, Chun-Yang Hu, Meng Chen, Ying Li, Yue-Shun Zhang
BACKGROUND: This study aimed to assess the efficacy and safety of remifentanil as a general anesthetic during cesarean delivery. MATERIAL AND METHODS: Fifty women with singleton pregnancies undergoing cesarean delivery were randomly divided into intervention and control groups, each group containing 25 subjects. Participants in the intervention group received remifentanil (infused at 2 μg/kg/h), whereas subjects in the control group were given dexmedetomidine (infused at 0...
December 2017: Medicine (Baltimore)
Valery V Khramtsov
EPR-based spectroscopic and imaging techniques allow for the study of free radicals - molecules with one or more unpaired electrons. Biological EPR applications include detection of endogenous biologically-relevant free radicals as well as use of specially designed exogenous radicals to probe local microenvironments. This forum issue focuses on recent advances in the field of <i>in vivo</i> EPR applications discussed at the International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems (EPR-2017)...
January 5, 2018: Antioxidants & Redox Signaling
Ákos Nemecz, Haidai Hu, Zaineb Fourati, Catherine Van Renterghem, Marc Delarue, Pierre-Jean Corringer
The Gloeobacter violaceus ligand-gated ion channel (GLIC) has been extensively studied by X-ray crystallography and other biophysical techniques. This provided key insights into the general gating mechanism of pentameric ligand-gated ion channel (pLGIC) signal transduction. However, the GLIC is activated by lowering the pH and the location of its putative proton activation site(s) still remain(s) unknown. To this end, every Asp, Glu, and His residue was mutated individually or in combination and investigated by electrophysiology...
December 27, 2017: PLoS Biology
Chao-Hui Feng, Yoshio Makino, Masatoshi Yoshimura, Dang Quoc Thuyet, Juan Francisco García-Martín
The potential of hyperspectral imaging with wavelengths of 380 to 1000 nm was used to determine the pH of cooked sausages after different storage conditions (4 °C for 1 d, 35 °C for 1, 3, and 5 d). The mean spectra of the sausages were extracted from the hyperspectral images and partial least squares regression (PLSR) model was developed to relate spectral profiles with the pH of the cooked sausages. Eleven important wavelengths were selected based on the regression coefficient values. The PLSR model established using the optimal wavelengths showed good precision being the prediction coefficient of determination (Rp2 ) 0...
December 26, 2017: Journal of Food Science
Chaobing Luo, Anxuan Liu, Wencong Long, Hong Liao, Yaojun Yang
In order to reduce the Cyrtotrachelus buqueti population, which is a serious pest in the bamboo industry, a range of approaches is required, which will be dependent on diverse gene expression influenced by environmental factors. In this study, samples from two regions of China, Muchuan in Sichuan Province and Chishui in Guizhou Province, were investigated through RNA-seq. Approximately 44 million high-quality reads were generated and 94.2% of the data was mapped to the transcriptome. A total of 15,641 out of the 29,406 identified genes were predicted...
December 21, 2017: Gene
Maik Hilgarth, Jürgen Behr, Rudi F Vogel
AIMS: This study aimed to explore the discriminatory power of MALDI-TOF MS as a high-throughput method to monitor growth dynamics of meat-spoiling bacteria on modified atmosphere packaged (MAP) beef and to differentiate psychrophilic and psychrotrophic spoilage-associated strains. METHODS AND RESULTS: MAP beef steaks were incubated for 21 days at constant 4 and 10 °C. Development of headspace gas composition, pH, CFU and spoilage-associated microbial composition were monitored...
December 14, 2017: Journal of Applied Microbiology
Zhuang Wang, Joris T K Quik, Lan Song, Marja Wouterse, Willie J G M Peijnenburg
Comprehensive experimental quantification and mapping of the aggregation and dispersion state of engineered nanoparticles (NPs) in the presence of humic substances (HS) is a great challenge. Dissipative particle dynamic (DPD) simulation was adopted to investigate the aggregation and dispersion mechanisms of NPs in the presence of a HS analogue. Twelve different types of NPs including two metal-based NPs, seven metal oxide-based NPs, and three carbon-based NPs in pure water (pH 3.0) and algae medium (pH 8.0) in the presence of a HS analogy were selected for experimental verification of the DPD simulation results...
December 14, 2017: Environmental Toxicology and Chemistry
Kelun Wang, Yi Luo, Toshiyuki Asaki, Thomas Graven-Nielsen, Brian E Cairns, Thomas Arendt-Nielsen, Lars Arendt-Nielsen
BACKGROUND AND PURPOSE: Acid has long been thought to play an important role in the pain process. Animal study showed that repeated acid stimulation induced central sensitization. The purpose of the study is to investigate muscle pain and hyperalgesia evoked by intramuscular infusion of saline at different pH levels, and to compare the effect of a single versus repeated acid infusions. METHODS: Twenty healthy subjects received infusions of buffered saline (pH 5...
October 2017: Scandinavian Journal of Pain
Brenda R de Camargo, Nico J Claassens, Betania Ferraz Quirino, Eliane F Noronha, Servé W M Kengen
An extensive list of putative cellulosomal enzymes from C. thermocellum is now available in the public databanks, however, most of these remain unvalidated with regard to their activity and expression control mechanisms. This is particularly true of those enzymes putatively involved in hemicellulose deconstruction. Our research group has been working on mapping and characterization of glycoside hydrolases produced by C. thermocellum B8, that are critical for lignocellulosic biomass deconstruction. One of the identified genes expressed during growth on sugar cane bagasse and straw is axb8, which encodes a putative cellulosomal GH43_29 α-arabinofuranosidase (EC 3...
February 2018: Enzyme and Microbial Technology
Xin Sha, Sisen Zhang, Hongwei Wang, Yingxin Cen, Wei Song, Jing Li, Lixiang Wang
OBJECTIVE: To analyze the effect of active abdominal compression-decompression cardiopulmonary resuscitation (AACD-CPR) and standard cardiopulmonary resuscitation (STD-CPR) on oxygen metabolism and prognosis of patient with cardiac arrest (CA), and to evaluate the treatment effect of AACD-CPR. METHODS: Patients with CA, CA time less than 30 minutes, and without STD-CPR and AACD-CPR contraindications admitted to the Zhengzhou People's Hospital from October 1st 2015 to May 31st 2017 were enrolled...
December 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Susan A Gerbi
THE Genetics Society of America's (GSA) George W. Beadle Award honors individuals who have made outstanding contributions to the community of genetics researchers and who exemplify the qualities of its namesake. The 2017 recipient is Susan A. Gerbi, who has been a prominent leader and advocate for the scientific community. In the course of her research on DNA replication, Gerbi helped develop the method of Replication Initiation Point (RIP) mapping to map replication origins at the nucleotide level, improving resolution by two orders of magnitude...
December 2017: Genetics
Faten Telahigue, Belgacem Agoubi, Fayza Souid, Adel Kharroubi
The present study integrates hydrogeological, hydrochemical and radiogenic data of groundwater samples taken from the Plio-Quaternary unconfined aquifer of Jerba Island, southeastern Tunisia, in order to interpret the spatial variations of the groundwater quality and identify the main hydrogeochemical factors responsible for the high ion concentrations and radon-222 content in the groundwater analysed. Thirty-nine groundwater samples were collected from open wells widespread on the island. Physical parameters (EC, pH, TDS and T °) were measured, major ions (Ca2+, Mg2+, Na+, K+, Cl-, SO42-, NO3- and HCO3-) were analysed and 222Rn concentrations were determined using a RAD7-H2O...
December 1, 2017: Journal of Environmental Radioactivity
Yimin Zhang, Libo Qin, Yanwei Mao, David L Hopkins, Guangxing Han, Lixian Zhu, Xin Luo
In this study, carbon monoxide (CO) modified atmosphere packaging (MAP) was used to increase redness (a*) and lightness (L*) values of dark cutting beef (ultimate pH (pHu)≥6.10), compared to normal pHu beef and intermediate pHu beef (pH: 5.40-5.79; pH: 5.80-6.09, respectively) during 20 d chilled storage. Compared with HiOx-MAP, CO-MAP exhibited similar color improvement effects (increased L*, a*, b* values) for all pHu beef groupings. The metmyoglobin (MetMb) content was lower under CO-MAP than that of HiOx-MAP in normal pHu beef, but opposite effects were observed in dark, high pH beef...
November 17, 2017: Meat Science
Michael S Lallemand, Donald M Moe, John M McClellan, Michael Loughren, Shannon Marko, Matthew J Eckert, Matthew J Martin
BACKGROUND: The acute coagulopathy of trauma is often accompanied by hyperfibrinolysis. TXA can reverse this phenomenon, and, when given early, decreases mortality from bleeding. Establishing IV access can be difficult in trauma and IO access is often preferred for drug administration. Currently, there is no data on the efficacy of IO administered TXA. Our objectives were to compare serum concentrations of tranexamic acid (TXA) when given IV and intraosseous (IO) and to compare the efficacy of IO administered TXA to IV at reversing hyperfibrinolysis METHODS: Using a porcine hemorrhage and ischemia-reperfusion (IR) model, 18 swine underwent hemorrhagic shock followed by a tissue plasminogen activator (tPA) infusion to induce hyperfibrinolysis...
December 1, 2017: Journal of Trauma and Acute Care Surgery
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"