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https://www.readbyqxmd.com/read/28718095/changes-in-sub-soil-river-water-quality-upon-its-open-storage-a-case-study
#1
A K Mohanty, K K Satpathy, M V R Prasad
A study was carried out to investigate the changes in the physicochemical and biological properties of sub-soil river water upon its storage in a man-made reservoir. Palar sub-soil and reservoir water samples were collated fortnightly for a period of 5 years (2010-2014). The open reservoir is used as a reliable raw water source for condenser cooling systems and for the demineralizing (DM) plant input of Fast Breeder Test Reactor (FBTR), Madras Atomic Power Station (MAPS), and other laboratories at Kalpakkam, southeast coast of India...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28714513/in-vivo-evaluation-of-tumour-acidosis-for-assessing-the-early-metabolic-response-and-onset-of-resistance-to-dichloroacetate-by-using-magnetic-resonance-ph-imaging
#2
Annasofia Anemone, Lorena Consolino, Laura Conti, Francesca Reineri, Federica Cavallo, Silvio Aime, Dario Livio Longo
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo extracellular tumour pH. We examined whether MRI-CEST pH mapping can monitor in vivo changes in tumour acidosis for assessing treatment response to DCA. Cell viability and extracellular pH were assessed in TS/A breast cancer cells treated with 1-10 mM DCA for 24 h in normoxia or hypoxia (1% O2) conditions...
August 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28709157/hypotensive-resuscitation-with-hypertonic-saline-dextran-improves-survival-in-a-rat-model-of-hemorrhagic-shock-at-high-altitude
#3
Jingxiang Zhao, Guoxing You, Bo Wang, Zhenwei Sun, Zhen Wang, Sha Xia, Lian Zhao, Hong Zhou
OBJECTIVE: To compare the efficacy of hypotensive resuscitation with hypertonic saline dextran 70 (HSD) and lactated Ringer (LR) solutions in a rat model of hemorrhagic shock at a simulated altitude of 4,000 m. METHODS: Anesthetized rats were bled to maintain their mean arterial pressure (MAP) at 45 mm Hg for 1 h. The distal quarter of the tail was then amputated to allow free blood loss; rats were simultaneously resuscitated with 4 mL kg HSD (HSD group, n = 10) or 4 mL kg LR (LR group, n = 10), followed by hypotensive resuscitation with LR to maintain MAP at 55 to 60 mm Hg for 1 h...
August 2017: Shock
https://www.readbyqxmd.com/read/28709068/simultaneous-phosphorus-and-nitrogen-recovery-from-anaerobically-digested-sludge-using-a-hybrid-system-coupling-hydrothermal-pretreatment-with-map-precipitation
#4
Yang Yu, Zhongfang Lei, Tian Yuan, Yong Jiang, Nan Chen, Chuanping Feng, Kazuya Shimizu, Zhenya Zhang
In order to dispose excess sewage sludge and recycle its nitrogen and phosphorus resources, hydrothermal pretreatment coupling with MgNH4PO3·6H2O (MAP) crystallization was investigated. Hydrothermal pretreatment at different temperature and residence time were performed on anaerobically digested sludge to maximize phosphate and ammonium release and their recovery from the resultant hydrolysate. Results showed that hydrothermal pretreatment at 200°C for 30min favored phosphate and ammonium release achieving phosphate and ammonium concentrations at 579...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28709039/agricultural-management-of-an-oxisol-affects-accumulation-of-heavy-metals
#5
Guilherme Deomedesse Minari, David Luciano Rosalen, Mara Cristina Pessôa da Cruz, Wanderley José de Melo, Lucia Maria Carareto Alves, Luciana Maria Saran
Soil contamination may result from the inadequate disposal of substances with polluting potential or prolonged agricultural use. Therefore, cadmium (Cd), chromium (Cr) and nickel (Ni) concentrations were assessed in a Eutroferric Red Oxisol under a no-tillage farming system with mineral fertilizer applications, a conventional tillage system with mineral fertilizer application and a conventional tillage system with sewage sludge application in an area used for agriculture for more than 80 years. We evaluated the spatial distributions of these elements in the experimental area and the effect of the different management practices on the soil retention of these metals...
July 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28704662/assessment-of-heavy-metal-contamination-in-sediment-at-sukinda-ultramafic-complex-using-haadf-stem-analysis
#6
Sk Md Equeenuddin, Binaya Kumar Pattnaik
The Sukinda ultramafic complex in Odisha has the largest chromite reserve in India. Sediment derived from ultramafic rocks has been enriched with various metals. Further, mining activities enhance the influx of metals into sediment by dumping mine overburden and tailings in the open area. Metal concentration in sediment is found in order of CrTotal(Cr) > Mn > Ni > Co > Zn > Cu with average concentration 26,778 mg/kg, 3098 mg/kg, 1813 mg/kg, 184 mg/kg, 116 mg/kg and 44 mg/kg respectively...
June 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/28692057/the-interactome-of-metabolic-enzyme-carbonic-anhydrase-ix-reveals-novel-roles-in-tumor-cell-migration-and-invadopodia-mmp14-mediated-invasion
#7
M Swayampakula, P C McDonald, M Vallejo, E Coyaud, S C Chafe, A Westerback, G Venkateswaran, J Shankar, G Gao, E M N Laurent, Y Lou, K L Bennewith, C T Supuran, I R Nabi, B Raught, S Dedhar
Carbonic anhydrase IX (CAIX) is a hypoxia inducible factor 1-induced, cell surface pH regulating enzyme with an established role in tumor progression and clinical outcome. However, the molecular basis of CAIX-mediated tumor progression remains unclear. Here, we have utilized proximity dependent biotinylation (BioID) to map the CAIX 'interactome' in breast cancer cells in order to identify physiologically relevant CAIX-associating proteins with potential roles in tumor progression. High confidence proteins identified include metabolic transporters, β1 integrins, integrin-associated protein CD98hc and matrix metalloprotease 14 (MMP14)...
July 10, 2017: Oncogene
https://www.readbyqxmd.com/read/28690618/identification-of-major-effect-qtls-for-agronomic-traits-and-cssls-in-rice-from-swarna-oryza-nivara-derived-backcross-inbred-lines
#8
Malathi Surapaneni, Divya Balakrishnan, Sukumar Mesapogu, Krishnam Raju Addanki, Venkateswara Rao Yadavalli, V G N Tripura Venkata, Sarla Neelamraju
Backcross inbred lines (BILs) derived from elite x wild crosses are very useful for basic studies and breeding. The aim of this study was to map quantitative trait loci (QTLs) associated with yield and related traits and to identify chromosomal segment substitution lines (CSSLs) from unselected BC2F8 BILs of Swarna/Oryza nivara IRGC81848. In all, 94 BILs were field evaluated in 2 years (wet seasons, 2014 and 2015) for nine traits; days to 50% flowering, days to maturity (DM), plant height (PH), number of tillers, number of productive tillers, panicle weight, yield per plant, bulk yield, and biomass...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28690182/functional-characterization-of-a-soluble-nadph-cytochrome-p450-reductase-from-fusarium-graminearum
#9
Thomas Etzerodt, Karl Wetterhorn, Giuseppe Dionisio, Ivan Rayment
Fusarium head blight is a devastating disease in wheat caused by some fungal pathogens of the Fusarium genus mainly F. graminearum, due to accumulation of toxic trichothecenes. Most of the trichothecene biosynthetic pathway has been mapped, although some proteins of the pathway remain uncharacterized, including an NADPH-cytochrome P450 reductase. We subcloned a F. graminearum cytochrome P450 reductase that might be involved in the trichothecene biosynthesis. It was expressed heterologously in E. coli as N-terminal truncated form with an octahistidine tag for purification...
July 6, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28689516/an-update-on-medium-and-low-abundant-blood-plasma-proteome-of-horse
#10
A Lepczyński, M Ożgo, A Dratwa-Chałupnik, P Robak, A Pyć, D Zaborski, A Herosimczyk
The main objectives of the study were to: (1) deeply analyse the serum protein composition of Equus caballus, (2) assess the effectiveness of the high-abundant protein depletion and improve the concentration of medium- and low-abundant proteins. The analysis were performed on the blood plasma of three healthy part-Arabian mares. The implementation of two-dimensional electrophoresis and matrix-assisted laser desorption/ionisation - time of flight mass spectrometry allowed us to establish a horse plasma proteome map...
July 10, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28687879/dynamic-sers-spectroscopy-for-the-in-situ-discrimination-of-xanthine-analogues-in-ternary-mixture
#11
Meiran Wu, Hao Li, Diya Lv, Feng Lu
Nowadays, there is a growing demand for analytical methods capable of providing rapid and simple identification of certain compounds in complex samples without a time-consuming separation process. In this report, a combinative strategy of dynamic surface-enhanced Raman scattering (DSERS) and two-dimensional correlation spectroscopy (2DCOS) analysis was demonstrated for the in situ identification of xanthine analogues in their ternary mixture. The DSERS method with an optimized initial pH value allows monitoring the distinct pH-dependent spectral evolution among analogues in real time, enhancing the spectral selectivity...
July 7, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28687588/the-carotid-baroreflex-modifies-the-pressor-threshold-of-the-muscle-metaboreflex-in-humans
#12
Masashi Ichinose, Tomoko K Ichinose-Kuwahara, Kazuhito Watanabe, Narihiko Kondo, Takeshi Nishiyasu
The purpose of present study was to test our hypothesis that unloading the carotid baroreceptors alters the threshold and gain of the muscle metaboreflex in humans. Ten healthy subjects performed a static handgrip exercise at 50% of maximum voluntary contraction. Contraction was sustained for 15, 30, 45 and 60 s, and was followed by 3 min of forearm circulatory arrest, during which forearm muscular pH is known to decrease linearly with increasing contraction time. The carotid baroreceptors were unloaded by applying 0...
July 7, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28686805/a-generalized-ratiometric-chemical-exchange-saturation-transfer-cest-mri-approach-for-mapping-renal-ph-using-iopamidol
#13
Yin Wu, Iris Y Zhou, Takahiro Igarashi, Dario L Longo, Silvio Aime, Phillip Zhe Sun
PURPOSE: To extend the pH detection range of iopamidol-based ratiometric chemical exchange saturation transfer (CEST) MRI at sub-high magnetic field and establish quantitative renal pH MRI. METHODS: Chemical exchange saturation transfer imaging was performed on iopamidol phantoms with pH of 5.5 to 8.0 and in vivo on rat kidneys (n = 5) during iopamidol administration at a 4.7 T. Iopamidol CEST effects were described using a multipool Lorentzian model. A generalized ratiometric analysis was conducted by ratioing resolved iopamidol CEST effects at 4...
July 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28681604/sequential-proton-loss-electron-transfer-in-deactivation-of-iron-iv-binding-protein-by-tyrosine-based-food-components
#14
Ning Tang, Leif H Skibsted
The iron(IV) binding protein ferrylmyoglobin, MbFe(IV)═O, was found to be reduced by tyrosine based food components in aqueous solution through a sequential proton loss electron transfer reaction mechanism without binding to the protein as confirmed by isothermal titration calorimetry. Dopamine and epinephrine are the most efficient food components reducing ferrylmyoglobin to oxymyoglobin, MbFe(II)O2, and metmyoglobin, MbFe(III), as revealed by multivariate curve resolution alternating least-squares with second order rate constants of 33...
July 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28674068/structural-and-functional-analysis-of-the-escherichia-coli-acid-sensing-histidine-kinase-evgs
#15
Hrishiraj Sen, Nikhil Aggarwal, Chibueze Ishionwu, Nosheen Hussain, Chandni Parmar, Mohammed Jamshad, Vassiliy N Bavro, Peter A Lund
The EvgS/EvgA two component system of E. coli is activated in response to low pH and alkali metals, and regulates many genes including the glutamate dependent acid resistance system and a number of efflux pumps. EvgS, the sensor kinase, is one of five unconventional histidine kinases in E. coli, and has a large periplasmic domain and a cytoplasmic PAS domain in addition to phospho-acceptor, HK and dimerization, internal receiver, and phosphotransfer domains. Mutations that constitutively activate the protein at pH 7 map to the PAS domain...
July 3, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28653507/t1-nuclear-magnetic-relaxation-dispersion-of-hyperpolarized-sodium-and-cesium-hydrogencarbonate-13-c
#16
Francisco M Martínez-Santiesteban, Thien Phuoc Dang, Heeseung Lim, Albert P Chen, Timothy J Scholl
In vivo pH mapping in tissue using hyperpolarized hydrogencarbonate-(13) C has been proposed as a method to study tumor growth and treatment and other pathological conditions related to pH changes. The finite spin-lattice relaxation times (T1 ) of hyperpolarized media are a significant limiting factor for in vivo imaging. Relaxation times can be measured at standard magnetic fields (1.5 T, 3.0 T etc.), but no such data are available at low fields, where T1 values can be significantly shorter. This information is required to determine the potential loss of polarization as the agent is dispensed and transported from the polarizer to the MRI scanner...
June 27, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28648673/clinical-evaluation-of-active-abdominal-lifting-and-compression-cardiopulmonary-resuscitation-in-patients-with-cardiac-arrest
#17
Min Li, Wei Song, Yan-Hong Ouyang, Duo-Hu Wu, Jun Zhang, Li-Xiang Wang, Jing Li
BACKGROUND: Chest compression is a standard recommendation during cardiopulmonary resuscitation (CPR). However, chest compression cannot be effectively applied under certain situations, such as chest wall deformity, rib fracture, or hemopneumothorax. An alternative method, abdominal compression, was reported to achieve better resuscitation outcomes in these patients. MATERIALS AND METHODS: A prospective study was performed in adult patients with cardiac arrest and anticipated ineffective chest compression (thoracic trauma, chest deformity, rib fracture, and hemopneumothorax)...
June 17, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28646311/removal-of-arsenic-iii-v-by-a-granular-mn-oxide-doped-al-oxide-adsorbent-surface-characterization-and-performance
#18
Kun Wu, Jin Zhang, Bing Chang, Ting Liu, Furong Zhang, Pengkang Jin, Wendong Wang, Xiaochang Wang
In order to remove arsenic (As) from contaminated water, granular Mn-oxide-doped Al oxide (GMAO) was fabricated using the compression method with the addition of organic binder. The analysis results of XRD, SEM, and BET indicated that GMAO was microporous with a large specific surface area of 54.26 m(2/)g, and it was formed through the aggregation of massive Al/Mn oxide nanoparticles with an amorphous pattern. EDX, mapping, FTIR, and XPS results showed the uniform distribution of Al/Mn elements and numerous hydroxyl groups on the adsorbent surface...
June 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28643782/group-additivity-pourbaix-diagrams-advocate-thermodynamically-stable-nanoscale-clusters-in-aqueous-environments
#19
Lindsay A Wills, Xiaohui Qu, I-Ya Chang, Thomas J L Mustard, Douglas A Keszler, Kristin A Persson, Paul Ha-Yeon Cheong
The characterization of water-based corrosion, geochemical, environmental and catalytic processes rely on the accurate depiction of stable phases in a water environment. The process is aided by Pourbaix diagrams, which map the equilibrium solid and solution phases under varying conditions of pH and electrochemical potential. Recently, metastable or possibly stable nanometric aqueous clusters have been proposed as intermediate species in non-classical nucleation processes. Herein, we describe a Group Additivity approach to obtain Pourbaix diagrams with full consideration of multimeric cluster speciation from computations...
June 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28639310/high-resolution-31-p-echo-planar-spectroscopic-imaging-in-vivo-at-7t
#20
Andreas Korzowski, Peter Bachert
PURPOSE: Conventional (31) P chemical shift imaging is time-consuming and yields only limited spatial resolution. The purpose of this study was to demonstrate feasibility of (31) P echo-planar spectroscopic imaging (EPSI) in vivo at 7T. METHODS: A 3D (31) P EPSI sequence with trapezoidal-shaped gradient pulses was implemented on a 7T MR scanner. To increase spectral width with reduced demand on gradient performance, a multishot approach was chosen. Acquisition weighting and (31) P-{(1) H} double resonance for nuclear Overhauser signal enhancement were applied to increase sensitivity...
June 21, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
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