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Rat achilles tendon

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https://www.readbyqxmd.com/read/29702290/construction-of-tendon-replacement-tissue-based-on-collagen-sponge-and-mesenchymal-stem-cells-by-coupled-mechano-chemical-induction-and-evaluation-of-its-tendon-repair-abilities
#1
Bingyu Zhang, Qing Luo, Bin Deng, Yasuyuki Morita, Yang Ju, Guanbin Song
Tissue engineering is an ideal therapeutic strategy for the development of functional tendon replacement tissue for tendon repair in the clinic. Currently, the synergistic roles of mechano-chemical factors and the mechanisms involved in tendon repair and regeneration are not fully understood. In this study, we developed a three-dimensional (3D) culture system based on a silicone chamber and collagen sponge scaffold that can deliver cyclic mechanical stretch and biochemical stimulation to bone marrow-derived mesenchymal stem cells (BMSCs) seeded on the scaffold...
April 24, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29696098/collagen-fibers-in-the-healing-process-of-rat-achilles-tendon-rupture-using-different-times-of-ultrasound-therapy
#2
Thiago Saikali Farcic, Cristiano Schiavinato Baldan, Aline Fernanda Perez Machado, Leonardo Affonso Massabki Caffaro, Igor Fagioli Bordello Masson, Raquel Aparecida Casarotto
Objective: The aim of this study was to evaluate the organization of collagen fibers in the healing process of rat Achilles tendon rupture using different times of ultrasound therapy (TUS). Approach: Forty Wistar rats were selected. Among these, 32 were submitted to total tenotomy of the calcaneous tendon and divided into 5 groups: control group (CG, n  = 8), without tenotomy or any treatment; tenotomy group ( n  = 8), with tenotomy and without treatment; TUS groups-TUS3 ( n  = 8), TUS4 ( n  = 8), and TUS5 ( n  = 8)-submitted to tenotomy and treated with TUS for 3, 4, and 5 min per effective radiating area (ERA), respectively...
April 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29678676/an-asymmetric-chitosan-scaffold-for-tendon-tissue-engineering-in-vitro-and-in-vivo-evaluation-with-rat-tendon-stem-progenitor-cells
#3
Erman Chen, Ling Yang, Chenyi Ye, Wei Zhang, Jisheng Ran, Deting Xue, Zhengke Wang, Zhijun Pan, Qiaoling Hu
The poor healing capacity and typically incomplete regeneration of injured tendons has made tendon repair as a primary clinical concern. Several methods for repairing injured tendons have been developed in the last decade. Tendon regeneration using current tissue engineering techniques requires advanced biomaterials to satisfy both microstructural and mechanical criteria. In this study, a novel chitosan (CS)-based scaffold with asymmetric structure was fabricated using a self-deposition technique. The fabricated scaffolds were assessed with regard to the microstructural and mechanical demands of cell ingrowth and the prevention of peritendinous adhesion...
April 17, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29676227/chemically-modified-mrna-modified-rna-application-for-treatment-of-achilles-tendon-defects
#4
Elmar Herbst, Florian Imhoff, Peter Föhr, Stefan Milz, Christian Plank, Carsten Rudolph, Günther Hasenpusch, Johannes Geiger, Manish Aneja, Karoline Groth, Stephan Vogt, Andreas Imhoff, Andreas Schmitt
Different regenerative medicine approaches for tendon healing exist. Recently, especially gene therapy gained popularity. However, potential mutagenic and immunologic effects might prevent from translation to clinical research. Chemically modified mRNA (cmRNA) might bypass these limitations of gene therapy. Therefore, the purpose of this study was to evaluate the early healing properties of Achilles tendon defects in rats treated with basic fibroblast growth factor (bFGF) cmRNA. Forty male Lewis rats were used for the study and randomly assigned to the two study groups: 1) treatment with cmRNA coding for bFGF, 2) non-coding cmRNA control...
April 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29674493/effect-of-electroacupuncture-stimulation-on-long-term-recovery-following-achilles-tendon-rupture-in-a-rat-model
#5
Miwa Imaeda, Tatsuya Hojo, Hiroshi Kitakoji, Kazuto Tanaka, Megumi Itoi, Motohiro Inoue
AIMS: In this study we examined the effect of electroacupuncture (EA) stimulation on the mechanical strength of the rat Achilles tendon after long-term recovery. METHODS: Using 20 rats, an Achilles tendon rupture model was created in an invasive manner. The rats were assigned to one of three groups, that received EA treatment (EA group), minimal acupuncture (MA group) or remained untreated (Control group). In the EA group, EA stimulation (5 ms, 50 Hz, 20 µA, 20 min) was applied to the rupture region over a period of 90 days (five times/week)...
April 19, 2018: Acupuncture in Medicine: Journal of the British Medical Acupuncture Society
https://www.readbyqxmd.com/read/29672303/bevacizumab-improves-achilles-tendon-repair-in-a-rat-model
#6
Herbert Tempfer, Alexandra Kaser-Eichberger, Christine Lehner, Renate Gehwolf, Stefanie Korntner, Nadja Kunkel, Andrea Wagner, Moritz Gruetz, Ludwig M Heindl, Falk Schroedl, Andreas Traweger
BACKGROUND/AIMS: Effective wound-healing generally requires efficient re-vascularization after injury, ensuring sufficient supply with oxygen, nutrients, and various cell populations. While this applies to most tissues, tendons are mostly avascular in nature and harbor relatively few cells, probably contributing to their poor regenerative capacity. Considering the minimal vascularization of healthy tendons, we hypothesize that controlling angiogenesis in early tendon healing is beneficial for repair tissue quality and function...
April 16, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29620182/response-of-decorin-to-different-intensity-treadmill-running
#7
Shao-Yong Xu, Sheng-Yao Liu, Lei Xu, Song-Yun Deng, Yong-Bin He, Shu-Fen Li, Guo-Xin Ni
Decorin is widely understood to affect collagen fibrillogenesis. However, little is understood about its response to various mechanical loading conditions. In the present study, 36 Wistar rats were randomly divided into control (CON), moderate treadmill running (MTR) and strenuous treadmill running (STR) groups. Animals in the MTR and STR groups were subjected to a 4‑ or 8‑week treadmill running protocol. Subsequently, all Achilles tendons were harvested to perform histological and biochemical analyses...
March 28, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29608156/effects-of-allogeneic-platelet-rich-plasma-prp-on-the-healing-process-of-sectioned-achilles-tendons-of-rats-a-methodological-description
#8
Laura Greimers, Pierre V Drion, Alain Colige, Vincent Libertiaux, Vincent Denoël, Christelle Lecut, André Gothot, Jean-François Kaux
This article describes the experimental procedures used to observe if PRP can positively affect tendon healing. There are 4 main steps to follow: induce a lesion in the Achilles tendon; prepare PRP and inject it (or the saline solution); remove the tendon; and perform biomechanical, molecular, and histological evaluations. At each step, all the procedures and methods are described in detail, so they can be reproduced easily. Achilles tendons have been surgically sectioned (removal of a 5-mm long section). Afterwards, PRP or saline solution was injected to study whether PRP has a positive effect on the healing of the tendon...
March 19, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29602657/tendon-adhesions-a-novel-method-of-objectively-measuring-adhesions-by-assessing-tendon-glide-through-a-soft-tissue-envelope-in-a-rat-model
#9
Aimee Riley, Jonathan E Isaacs, Ilvy Cotterell, Jeffrey Stromberg, Satya Mallu, Gaurangkumar Patel
PURPOSE: To develop a rat model of extra-synovial tendon adhesions that will enable accurate testing of scar barriers and adhesion inhibiting treatments to facilitate future research. METHODS: Thirty-six 6-month-old male Sprague-Dawley rats were randomized to one of the 3 groups of 12. In Group A, the middle one-third portion of the left Achilles tendon was excised. In Group B, the tendon and soft tissue bed was abraded with steel wool. In Group C, a silk suture was sewn along the tendon...
March 28, 2018: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29547593/interleukin-6-promotes-proliferation-but-inhibits-tenogenic-differentiation-via-the-janus-kinase-signal-transducers-and-activators-of-transcription-3-jak-stat3-pathway-in-tendon-derived-stem-cells
#10
Siwei Chen, Ganming Deng, Kaiqun Li, Haonan Zheng, Gang Wang, Bin Yu, Kairui Zhang
BACKGROUND Previous studies demonstrated that tendon-derived stem cells (TDSCs) were vital healing cells and that mRNA expression of anti-inflammatory cytokine IL-6 was significantly upregulated in injured tendons. The aim of the present study was to investigate the effects of IL-6 on the TDSCs in vitro. MATERIAL AND METHODS TDSCs isolated from the Achilles tendons in SD rats were co-cultured with various concentrations of IL-6. Cell proliferation, cell cycle analysis, quantitative real-time PCR, western blotting analysis, and statistical analysis were used in the study...
March 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29534523/exploring-the-in-vivo-anti-inflammatory-actions-of-simvastatin-loaded-porous-microspheres-on-inflamed-tenocytes-in-a-collagenase-induced-animal-model-of-achilles-tendinitis
#11
Chandong Jeong, Sung Eun Kim, Kyu-Sik Shim, Hak-Jun Kim, Mi Hyun Song, Kyeongsoon Park, Hae-Ryong Song
Tendon rupture induces an inflammatory response characterized by release of pro-inflammatory cytokines and impaired tendon performance. This study sought to investigate the therapeutic effects of simvastatin-loaded porous microspheres (SIM/PMSs) on inflamed tenocytes in vitro and collagenase-induced Achilles tendinitis in vivo. The treatment of SIM/PMSs in lipopolysaccharide (LPS)-treated tenocytes reduced the mRNA expressions of pro-inflammatory cytokines (Matrix metalloproteinase-3 ( MMP-3 ), cyclooxygenase-2 ( COX-2 ), interleukin-6 ( IL-6 ), and tumor necrosis factor-α ( TNF-α ))...
March 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29526155/local-application-of-tranexamic-acid-affects-tendon-healing-negatively-in-the-late-period
#12
Alper Çıraklı, Pınar Naile Gürgör, Erdal Uzun, Havva Erdem, Soner Çankaya, Orhan Baş
OBJECTIVES: This study aims to evaluate the potential adverse effects of tranexamic acid (TA) on tendon healing. MATERIALS AND METHODS: Twelve male Wistar-Albino rats (weighing 300 g to 350 g) were used in the study. Rats were divided into two groups. Right legs of the rats were determined as the TA group and left legs as the serum physiologic (SP) group. Bilateral Achilles tenotomy was performed and surgically repaired. For the right side, 1 mL of TA and for the left side, 1 mL of SP were applied...
April 2018: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
https://www.readbyqxmd.com/read/29526154/effects-of-low-molecular-weight-heparin-and-rivaroxaban-on-rat-achilles-tendon-healing
#13
Yılmaz Eren, Oktay Adanır, Yaşar Mahsut Dinçel, Erdinç Genç, Yunus Ziya Arslan, Aysel Çağlar
OBJECTIVES: This study aims to compare the histopathological and biomechanical effects of low molecular weight heparin (LMWH) and rivaroxaban, which are frequently used in orthopedic surgery for thromboembolic prophylaxis, on rat Achilles tendon healing. MATERIALS AND METHODS: In the study, 36 adult, male Sprague Dawley rats weighing between 300 g and 400 g were used. Three groups were formed including 12 rats in each. Achilles tendons of all rats were cut and repaired...
April 2018: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
https://www.readbyqxmd.com/read/29505741/the-role-of-the-paratenon-in-achilles-tendon-healing-a-study-in-rats
#14
Sebastian A Müller, Christopher H Evans, Patricia E Heisterbach, Martin Majewski
BACKGROUND: The role of the paratenon in tendon healing is unknown. The present study compares healing in the presence or absence of the paratenon in an Achilles tendon defect model in rats. HYPOTHESIS: Resection of the paratenon impairs tendon healing. STUDY DESIGN: Controlled laboratory study. METHODS: Sixty skeletally mature Sprague Dawley rats were randomly assigned to either a resected paratenon (RP) group or an intact paratenon (IP) group...
April 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29493345/negative-effect-of-zoledronic-acid-on-tendon-to-bone-healing
#15
Geir Aasmund Hjorthaug, Endre Søreide, Lars Nordsletten, Jan Erik Madsen, Finn P Reinholt, Sanyalak Niratisairak, Sigbjørn Dimmen
Background and purpose - Outcome after ligament reconstruction or tendon repair depends on secure tendon-to-bone healing. Increased osteoclastic activity resulting in local bone loss may contribute to delayed healing of the tendon-bone interface. The objective of this study was to evaluate the effect of the bisphosphonate zoledronic acid (ZA) on tendon-to-bone healing. Methods - Wistar rats (n = 92) had their right Achilles tendon cut proximally, pulled through a bone tunnel in the distal tibia and sutured anteriorly...
June 2018: Acta Orthopaedica
https://www.readbyqxmd.com/read/29454565/investigation-of-the-biomechanical-and-histopathological-effects-of-autologous-conditioned-serum-on-healing-of-achilles-tendon
#16
Erdinç Genç, Ozan Beytemur, Serdar Yuksel, Yılmaz Eren, Aysel Çağlar, Bedri Onur Küçükyıldırım, Mehmet Akif Güleç
OBJECTIVE: The aim of this study to evaluate the effects of autologous conditioned serum (ACS) on the healing of transected rat Achilles tendons via the assessment of biomechanical and histological parameters. METHODS: The study was conducted on 45 male Sprague-Dawley rats. Five rats were used as donors for ACS preparation. Animals were randomly assigned to the experimental or control group. In both groups, the Achilles tendon was cut transversally and then sutured...
February 14, 2018: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/29447959/effects-of-maturation-and-advanced-glycation-on-tensile-mechanics-of-collagen-fibrils-from-rat-tail-and-achilles-tendons
#17
Rene B Svensson, Stuart T Smith, Patrick J Moyer, S Peter Magnusson
Connective tissues are ubiquitous throughout the body and consequently affect the function of many organs. In load bearing connective tissues like tendon, the mechanical functionality is provided almost exclusively by collagen fibrils that in turn are stabilized by covalent cross-links. Functionally distinct tendons display different cross-link patterns, which also change with maturation, but these differences have not been studied in detail at the fibril level. In the present study, a custom built nanomechanical test platform was designed and fabricated to measure tensile mechanics of individual fibrils from rat tendons...
April 1, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29428676/controlled-release-of-collagen-binding-sdf-1%C3%AE-from-the-collagen-scaffold-promoted-tendon-regeneration-in-a-rat-achilles-tendon-defect-model
#18
Jie Sun, Chenchen Mou, Qin Shi, Bing Chen, Xianglin Hou, Wen Zhang, Xiaoran Li, Yan Zhuang, Jiajia Shi, Yanyan Chen, Jianwu Dai
It had been demonstrated that stromal cell-derived factor-1α (SDF-1α) could promote in situ tendon regeneration by recruiting endogenous cells. However, native SDF-1α diffuses too fast in vivo, reducing its local concentration and efficacy. In this study, we prepared a recombinant SDF-1α containing a collagen-binding domain (CBD-SDF-1α) and developed a functional collagen scaffold by tethering CBD-SDF-1α on the collagen scaffold for in situ tendon regeneration. CBD-SDF-1α could induce the migration of mesenchymal stem cells, dermal fibroblasts and Achilles tendon fibroblasts in vitro, and achieve controlled release from the collagen scaffold...
April 2018: Biomaterials
https://www.readbyqxmd.com/read/29387650/the-effect-of-strain-and-age-on-the-mechanical-properties-of-rat-achilles-tendons
#19
Emily C Vafek, Johannes F Plate, Eric Friedman, Sandeep Mannava, Aaron T Scott, Kerry A Danelson
Background: Achilles tendon (AT) ruptures are common in the middle age population; however, the pathophysiology and influence of age on AT ruptures is not fully understood. This study evaluates the effect and interactions between, strain and age on the in vitro biomechanical properties of ATs. Methods: Bilateral ATs were harvested from 17 young (8 months) and 14 middle-aged (24 months) rats and underwent stress-relaxation using Fung's quasilinear viscoelastic (QLV) modeling and load-to-failure testing...
July 2017: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/29351870/de-novo-generation-in-an-in-vivo-rat-model-and-biomechanical-characterization-of-autologous-transplants-for-ligament-and-tendon-reconstruction
#20
Marc Soubeyrand, Elisabeth Laemmel, Nathalie Maurel, Amadou Diop, Thierry Lazure, Jacques Duranteau, Eric Vicaut
BACKGROUND: Surgical reconstruction of ligaments and tendons is frequently required in clinical practice. The commonly used autografts, allografts, or synthetic transplants present limitations in terms of availability, biocompatibility, cost, and mechanical properties that tissue bioengineering aims to overcome. It classically combines an exogenous extracellular matrix with cells, but this approach remains complex and expensive. Using a rat model, we tested a new bioengineering strategy for the in vivo and de novo generation of autologous grafts without the addition of extracellular matrix or cells, and analyzed their biomechanical and structural properties...
February 2018: Clinical Biomechanics
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