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Cytochrome c oxidase

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https://www.readbyqxmd.com/read/28430780/the-importance-of-standardization-for-biodiversity-comparisons-a-case-study-using-autonomous-reef-monitoring-structures-arms-and-metabarcoding-to-measure-cryptic-diversity-on-mo-orea-coral-reefs-french-polynesia
#1
Emma Ransome, Jonathan B Geller, Molly Timmers, Matthieu Leray, Angka Mahardini, Andrianus Sembiring, Allen G Collins, Christopher P Meyer
The advancement of metabarcoding techniques, declining costs of high-throughput sequencing and development of systematic sampling devices, such as autonomous reef monitoring structures (ARMS), have provided the means to gather a vast amount of diversity data from cryptic marine communities. However, such increased capability could also lead to analytical challenges if the methods used to examine these communities across local and global scales are not standardized. Here we compare and assess the underlying biases of four ARMS field processing methods, preservation media, and current bioinformatic pipelines in evaluating diversity from cytochrome c oxidase I metabarcoding data...
2017: PloS One
https://www.readbyqxmd.com/read/28428045/sco2-deficient-mice-develop-increased-adiposity-and-insulin-resistance
#2
Shauna Hill, Sathyaseelan S Deepa, Kavithalakshmi Sataranatarajan, Pavithra Premkumar, Daniel Pulliam, Yuhong Liu, Vanessa Y Soto, Kathleen E Fischer, Holly Van Remmen
Cytochrome c oxidase (COX) is an essential transmembrane protein complex (Complex IV) in the mitochondrial respiratory electron chain. Mutations in genes responsible for the assembly of COX are associated with Leigh syndrome, cardiomyopathy, spinal muscular atrophy and other fatal metabolic disorders in humans. Previous studies have shown that mice lacking the COX assembly protein Surf1 (Surf1(-/-) mice) paradoxically show a number of beneficial metabolic phenotypes including increased insulin sensitivity, upregulation of mitochondrial biogenesis, induction of stress response pathways and increased lifespan...
April 17, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28427478/morphological-and-molecular-characteristics-of-malayfilaria-sofiani-uni-mat-udin-takaoka-n-g-n-sp-nematoda-filarioidea-from-the-common-treeshrew-tupaia-glis-diard-duvaucel-mammalia-scandentia-in-peninsular-malaysia
#3
Shigehiko Uni, Ahmad Syihan Mat Udin, Takeshi Agatsuma, Weerachai Saijuntha, Kerstin Junker, Rosli Ramli, Hasmahzaiti Omar, Yvonne Ai-Lian Lim, Sinnadurai Sivanandam, Emilie Lefoulon, Coralie Martin, Daicus Martin Belabut, Saharul Kasim, Muhammad Rasul Abdullah Halim, Nur Afiqah Zainuri, Subha Bhassu, Masako Fukuda, Makoto Matsubayashi, Masashi Harada, Van Lun Low, Chee Dhang Chen, Narifumi Suganuma, Rosli Hashim, Hiroyuki Takaoka, Mohd Sofian Azirun
BACKGROUND: The filarial nematodes Wuchereria bancrofti (Cobbold, 1877), Brugia malayi (Brug, 1927) and B. timori Partono, Purnomo, Dennis, Atmosoedjono, Oemijati & Cross, 1977 cause lymphatic diseases in humans in the tropics, while B. pahangi (Buckley & Edeson, 1956) infects carnivores and causes zoonotic diseases in humans in Malaysia. Wuchereria bancrofti, W. kalimantani Palmieri, Pulnomo, Dennis & Marwoto, 1980 and six out of ten Brugia spp. have been described from Australia, Southeast Asia, Sri Lanka and India...
April 20, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28425849/age-dependent-obesity-and-mitochondrial-dysfunction
#4
Qilong Oscar Yang Li, Ines Soro-Arnaiz, Julián Aragonés
Aging is associated with progressive visceral white adipose tissue (WAT) expansion both in human and mouse. Importantly, WAT enlargement is initiated early in life, suggesting that molecular mechanisms underlying age-dependent obesity are activated at early stages of lifetime. Our recent study found that age-dependent obesity was associated with a specific decline in mitochondrial complex IV activity, which leads to reduced fatty acid oxidation and subsequent adipocyte hypertrophy. At the molecular level, global mitochondrial complex IV inhibition was driven by hypoxia-inducible factor-1α (HIF1α)-mediated repression of some of its key subunits, including cytochrome c oxidase 5b (Cox5b)...
March 15, 2017: Adipocyte
https://www.readbyqxmd.com/read/28423703/artocarpin-an-isoprenyl-flavonoid-induces-p53-dependent-or-independent-apoptosis-via-ros-mediated-mapks-and-akt-activation-in-non-small-cell-lung-cancer-cells
#5
Ming-Horng Tsai, Ju-Fang Liu, Yao-Chang Chiang, Stephen Chu-Sung Hu, Lee-Fen Hsu, Yu-Ching Lin, Zih-Chan Lin, Hui-Chun Lee, Mei-Chuan Chen, Chieh-Liang Huang, Chiang-Wen Lee
Artocarpin has been shown to exhibit cytotoxic effects on different cancer cells, including non-small cell lung carcinoma (NSCLC, A549). However, the underlying mechanisms remain unclear. Here, we explore both p53-dependent and independent apoptosis pathways in artocarpin-treated NSCLC cells. Our results showed that artocarpin rapidly induced activation of cellular protein kinases including Erk1/2, p38 and AktS473. Inhibition of these protein kinases prevented artocarpin-induced cell death. Moreover, artocarpin-induced phosphorylation of these protein kinases and apoptosis were mediated by induction of reactive oxygen species (ROS), as pretreatment with NAC (a ROS scavenger) and Apocynin (a Nox-2 inhibitor) blocked these events...
March 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422135/genomic-evidence-of-hybridization-between-two-independent-invasions-of-european-green-crab-carcinus-maenas-in-the-northwest-atlantic
#6
N W Jeffery, C DiBacco, B F Wringe, R R E Stanley, L C Hamilton, P N Ravindran, I R Bradbury
Invasive species have been associated with significant negative impacts in their introduced range often outcompeting native species, yet the long-term evolutionary dynamics of biological invasions are not well understood. Hybridization, either among waves of invasion or between native and introduced populations, could alter the ecological and evolutionary impacts of invasions yet has rarely been studied in marine invasive species. The European green crab (Carcinus maenas) invaded eastern North America twice from northern and southern locations in its native range...
April 19, 2017: Heredity
https://www.readbyqxmd.com/read/28418073/evolved-changes-in-the-intracellular-distribution-and-physiology-of-muscle-mitochondria-in-high-altitude-native-deer-mice
#7
Sajeni Mahalingam, Grant B McClelland, Graham R Scott
High-altitude natives that have evolved to live in hypoxic environments provide a compelling system to understand how animals can overcome impairments in oxygen availability. We examined whether these include changes in mitochondrial physiology or intracellular distribution that contribute to hypoxia resistance in high-altitude deer mice (Peromyscus maniculatus). Mice from populations native to high- and low-altitudes were born and raised in captivity, and as adults were acclimated to normoxia or hypobaric hypoxia (equivalent to 4,300 m elevation)...
April 18, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28417591/effective-mosquito-and-arbovirus-surveillance-using-metabarcoding
#8
J Batovska, S E Lynch, N O I Cogan, K Brown, J M Darbro, E A Kho, M J Blacket
Effective vector and arbovirus surveillance requires timely and accurate screening techniques that can be easily upscaled. Next-generation sequencing (NGS) is a high-throughput technology that has the potential to modernise vector surveillance. When combined with DNA barcoding, it is termed 'metabarcoding'. The aim of our study was to establish a metabarcoding protocol to characterise pools of mosquitoes and screen them for virus. Pools contained 100 morphologically identified individuals, including one Ross River virus (RRV) infected mosquito, with three species present at different proportions: 1, 5, 94%...
April 18, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28413103/do-larval-types-affect-genetic-connectivity-at-sea-testing-hypothesis-in-two-sibling-marine-gastropods-with-contrasting-larval-development
#9
Maria Vittoria Modica, Valeria Russini, Giulia Fassio, Marco Oliverio
In marine environments, connectivity among populations of benthic invertebrates is provided primarily by dispersion of larvae, with the duration of pelagic larval phase (PLD) supposed to represent one of the major factor affecting connectivity. In marine gastropods, PLD is linked to specific larval development types, which may be entirely intracapsular (thus lacking a pelagic dispersal), or include a short pelagic lecithotrophic or a long planktotrophic phase. In the present study, we investigated two sibling species of the cosmopolitan neogastropod genus Columbella (commonly known as dove shells): Columbella adansoni Menke, 1853, from the Macaronesian Atlantic archipelagos, with planktotrophic development, and Columbella rustica Linnaeus, 1758, from the Mediterranean Sea, with intracapsular development...
April 4, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28412672/relationship-between-alzheimer-s-disease-and-mitochondria-coenzyme-ii-gene
#10
Chen Liangmei, Chen Ying, Liu Feihu, He Changjiang, Zhang Luying, He Dingxiong, Ao Lei
We aimed to investigate the relevance between Alzheimer's disease (AD) and gene mutations of mitochondrial cytochrome oxidase subunit III (COX3) and coenzyme II (ND2), and to provide genetic markers for the diagnosis of Alzheimer's disease (AD) and further provide some feasible basis for preventive treatment. Polymerase chain reaction-restriction fragment length polymorphism technique was used, and genotypes and gene frequencies were detected in 60 patients with Alzheimer's disease (AD), who meet the ICD-10 diagnostic criteria (AD group), 10 AD families and 60 normal old people (control group)...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28412453/in-vitro-mercaptopyruvate-sulfurtransferase-species-comparison-in-humans-and-common-laboratory-animals
#11
Bryant M Moeller, Daune L Crankshaw, Jacquie Briggs, Herbert Nagasawa, Steven E Patterson
Cyanide is a metabolic poison that inhibits cytochrome c oxidase. Its broad applications in manufacturing and history as an agent of warfare/terror highlight the limitations in approved cyanide antidotes for mass casualties. Sulfanegen, a pre-clinical antidote for cyanide poisoning, exploits an endogenous detoxification pathway and should be amenable to mass-casualty scenarios. Because human studies are unethical, determination of appropriate animal species as models in translational studies for FDA approval under the "Animal Rule" are critical...
April 12, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28409404/phenotypic-and-genotypic-identification-of-hard-ticks-of-the-genus-haemaphysalis-acari-ixodidae-in-peninsular-malaysia
#12
F C L Ernieenor, G Ernna, A Mariana
Morphotaxonomy based on phenotypic traits of immature hard ticks (Acari: Ixodidae) is a skill challenge and has prompted many inexperienced acarologists to adopt DNA-based methods for identifying and discriminating the species. The aim of this study is therefore to utilize COI gene for verifying the morphological status of Haemaphysalis ticks in Peninsular Malaysia. A total of 19 on-host ticks collected from four localities were first identified using specific illustrated taxonomic keys that lead to the genus of Haemaphysalis...
April 13, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28408971/long-term-effects-of-maternal-deprivation-on-redox-regulation-in-rat-brain-involvement-of-nadph-oxidase
#13
Branka Marković, Nevena V Radonjić, Gordana Jevtić, Tihomir Stojković, Milica Velimirović, Milan Aksić, Joko Poleksić, Tatjana Nikolić, Dubravka Aleksić, Vidosava Radonjić, Branislav Filipović, Nataša D Petronijević
Maternal deprivation (MD) causes perinatal stress, with subsequent behavioral changes which resemble the symptoms of schizophrenia. The NADPH oxidase is one of the major generators of reactive oxygen species, known to play a role in stress response in different tissues. The aim of this study was to elucidate the long-term effects of MD on the expression of NADPH oxidase subunits (gp91(phox), p22(phox), p67(phox), p47(phox), and p40(phox)). Activities of cytochrome C oxidase and respiratory chain Complex I, as well as the oxidative stress parameters using appropriate spectrophotometric techniques were analyzed...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28408098/population-genetics-of-the-schistosoma-snail-host-bulinus-truncatus-in-egypt
#14
Rima Zein-Eddine, Félicité F Djuikwo-Teukeng, Yasser Dar, Gilles Dreyfuss, Frederik Van den Broeck
The tropical freshwater snail Bulinus truncatus serves as an important intermediate host of several human and cattle Schistosoma species in many African regions. Despite some ecological and malacological studies, there is no information on the genetic diversity of B. truncatus in Egypt. Here, we sampled 70-100 snails in ten localities in Upper Egypt and the Nile Delta. Per locality, we sequenced 10 snails at a partial fragment of the cytochrome c oxidase subunit 1 gene (cox1) and we genotyped 25-30 snails at six microsatellite markers...
April 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28407939/dna-barcoding-coupled-to-hrm-analysis-as-a-new-and-simple-tool-for-the-authentication-of-gadidae-fish-species
#15
Telmo J R Fernandes, Joana Costa, M Beatriz P P Oliveira, Isabel Mafra
This work aimed to exploit the use of DNA mini-barcodes combined with high resolution melting (HRM) for the authentication of gadoid species: Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Theragra chalcogramma) and saithe (Pollachius virens). Two DNA barcode regions, namely cytochrome c oxidase subunit I (COI) and cytochrome b (cytb), were analysed in silico to identify genetic variability among the four species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407723/dna-barcodes-and-insights-into-the-phylogenetic-relationships-of-corvidae-aves-passeriformes
#16
Zuhao Huang, Rujue Ruan
DNA barcoding has become a promising tool for species identification and phylogeny in a wide range of animal taxa using mitochondrial cytochrome c oxidase subunit I (COI). The Corvidae (Aves: Passeriformes) is a species rich and morphologically diverse family. In the present study, we analyzed the COI barcodes of 39 species from 12 genera of Corvidae. COI gene was also used to examine phylogenetic relationships of Corvidae. Every species possessed a barcode distinct from that of other species. Kimura two-parameter distances were calculated between species barcodes...
April 13, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28407595/proteo-lipobeads-to-encapsulate-cytochrome-c-oxidase-from-paracoccus-denitrificans
#17
Andreas F Geiss, Christina Bliem, Pinar Frank, Ciril Reiner-Rozman, Justin Kewney, Michael Boersch, Renate L C Naumann
Proteo-lipobeads (PLBs) are investigated as cell-free model systems to encapsulate membrane proteins such as ion channels and transporters. PLBs are based on nickel nitrile tri-acetic acid (Ni-NTA)-functionalized agarose beads, onto which membrane proteins (MP) are bound via histidine(his)-tag. Composite beads thus obtained (subsequently called proteobeads) are dialyzed in the presence of lipid micelles to form PLBs. As an example we employed cytochrome c oxidase from P. denitrificans with a his-tag fused to the C-terminus of subunitI...
April 5, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28406159/sensitivity-and-accuracy-of-high-throughput-metabarcoding-methods-for-early-detection-of-invasive-fish-species
#18
Chelsea Hatzenbuhler, John R Kelly, John Martinson, Sara Okum, Erik Pilgrim
High-throughput DNA metabarcoding has gained recognition as a potentially powerful tool for biomonitoring, including early detection of aquatic invasive species (AIS). DNA based techniques are advancing, but our understanding of the limits to detection for metabarcoding complex samples is inadequate. For detecting AIS at an early stage of invasion when the species is rare, accuracy at low detection limits is key. To evaluate the utility of metabarcoding in future fish community monitoring programs, we conducted several experiments to determine the sensitivity and accuracy of routine metabarcoding methods...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28399302/genetic-diversity-and-phylogenetic-relationships-of-cytochrome-c-oxidase-subunit-i-in-cimex-hemipterus-hemiptera-cimicidae-populations-in-malaysia
#19
Siti Nor Ain Seri Masran, Abdul Hafiz Ab Majid
The tropical bed bug is scientifically recognized as a significant public health problem. While there is an increased awareness about their resurgence by medical and life science committees, efficient bed bug management still remains unresolved. The solution may soon arise, as information about bed bugs' infestation dynamics and systematics are becoming more distinguishable. Recent developments in studies about bed bugs are based on molecular intervention by determining their genetic variation and phylogeography...
February 8, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28399227/genetic-diversity-and-identification-of-palearctic-black-flies-in-the-subgenus-wilhelmia-diptera-simuliidae
#20
Abdullah Inci, Alparslan Yildirim, Onder Duzlu, Zuhal Onder, Arif Ciloglu, Gunther Seitz, Peter H Adler
Accurate species identifications are the essential first step in understanding the medical, economic, and ecological importance of black flies. The utility of DNA barcoding based on cytochrome c oxidase subunit 1 (COI) sequences was evaluated for identifying six common species of Palearctic black flies in the subgenus Wilhelmia, including several that are virulent pests. Chromosomally identified larvae from Turkey and Germany and COI sequences in GenBank were analyzed. Intraspecific genetic divergence was 0...
March 8, 2017: Journal of Medical Entomology
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