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AMA_1 vaccine

Qingli Niu, Zhijie Liu, Jifei Yang, Guiquan Guan, Yuping Pan, Jianxun Luo, Hong Yin
Apical membrane antigen-1 (AMA-1) has been described as a potential vaccine candidate in apicomplexan parasites. Here we characterize the ama-1 gene. The full-length ama-1 gene of Babesia sp. BQ1 (Lintan) (BLTAMA-1) is 1785 bp, which contains an open reading frame (ORF) encoding a 65-kDa protein of 594 amino acid residues; by definition, the 5' UTR precedes the first methionine of the ORF. Phylogenetic analysis based on AMA-1 amino acid sequences clearly separated Piroplasmida from other Apicomplexa parasites...
December 12, 2016: Parasitology
Abhik Saha, Sejal Chauhan, Tamishraha Bagchi
BACKGROUND: Recombinant proteins and vaccine candidates of Plasmodium vivax have met with limited success. One of the reasons could be their effect on monocytes which are important in malaria pathogenesis. Our aim was therefore to investigate the effect of selected recombinant malarial proteins on monocytes functions. METHODS: Phagocytosis rate and respiratory burst of healthy individuals' monocytes treated with antigens were examined. The homing capacity of monocytes was studied by examining the mRNA level of chemokine receptors from patients and healthy individuals...
August 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
Mahamadou A Thera, Drissa Coulibaly, Abdoulaye K Kone, Ando B Guindo, Karim Traore, Abdourhamane H Sall, Issa Diarra, Modibo Daou, Idrissa M Traore, Youssouf Tolo, Mady Sissoko, Amadou Niangaly, Charles Arama, Mounirou Baby, Bourema Kouriba, Mahamadou S Sissoko, Issaka Sagara, Ousmane B Toure, Amagana Dolo, Dapa A Diallo, Edmond Remarque, Roma Chilengi, Ramadhani Noor, Sanie Sesay, Alan Thomas, Clemens H Kocken, Bart W Faber, Egeruan Babatunde Imoukhuede, Odile Leroy, Ogobara K Doumbo
BACKGROUND: The safety and immunogenicity of PfAMA1, adjuvanted with Alhydrogel(®) was assessed in malaria-experienced Malian adults. The malaria vaccine, PfAMA1-FVO [25-545] is a recombinant protein Pichia pastoris-expressed AMA-1 from Plasmodium falciparum FVO clone adsorbed to Alhydrogel(®), the control vaccine was tetanus toxoid produced from formaldehyde detoxified and purified tetanus toxin. METHODS: A double blind randomized controlled phase 1 study enrolled and followed 40 healthy adults aged 18-55 years in Bandiagara, Mali, West Africa, a rural setting with intense seasonal transmission of P...
August 30, 2016: Malaria Journal
Chantal Ama Agbemabiese, Toyoko Nakagomi, Yen Hai Doan, Loan Phuong Do, Susan Damanka, George E Armah, Osamu Nakagomi
Understanding of the genetic diversity and evolution of Rotavirus A (RVA) strains, a common cause of severe diarrhoea in children, needs to be based on the analysis at the whole genome level in the vaccine era. This study sequenced the whole genomes of six representative G2P[4] strains detected in Ghana from 2008 to 2013, and analysed them phylogenetically with a global collection of G2P[4] strains and African non-G2P[4] DS-1-like strains. The genotype constellation of the study strains was G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2...
August 26, 2016: Infection, Genetics and Evolution
Ed Clarke, Yauba Saidu, Jane U Adetifa, Ikechukwu Adigweme, Mariama Badjie Hydara, Adedapo O Bashorun, Ngozi Moneke-Anyanwoke, Ama Umesi, Elishia Roberts, Pa Modou Cham, Michael E Okoye, Kevin E Brown, Matthias Niedrig, Panchali Roy Chowdhury, Ralf Clemens, Ananda S Bandyopadhyay, Jenny Mueller, David J Jeffries, Beate Kampmann
BACKGROUND: The introduction of the inactivated poliovirus vaccine (IPV) represents a crucial step in the polio eradication endgame. This trial examined the safety and immunogenicity of IPV given alongside the measles-rubella and yellow fever vaccines at 9 months and when given as a full or fractional dose using needle and syringe or disposable-syringe jet injector. METHODS: We did a phase 4, randomised, non-inferiority trial at three periurban government clinics in west Gambia...
August 2016: Lancet Global Health
Ricardo A Chaurio, M Andreína Pacheco, Omar E Cornejo, Ester Durrego, Craig E Stanley, Andreína I Castillo, Sócrates Herrera, Ananias A Escalante
Transmission-blocking (TB) vaccines are considered an important tool for malaria control and elimination. Among all the antigens characterized as TB vaccines against Plasmodium vivax, the ookinete surface proteins Pvs28 and Pvs25 are leading candidates. These proteins likely originated by a gene duplication event that took place before the radiation of the known Plasmodium species to primates. We report an evolutionary genetic analysis of a worldwide sample of pvs28 and pvs25 alleles. Our results show that both genes display low levels of genetic polymorphism when compared to the merozoite surface antigens AMA-1 and MSP-1; however, both ookinete antigens can be as polymorphic as other merozoite antigens such as MSP-8 and MSP-10...
June 2016: PLoS Neglected Tropical Diseases
Alessandra Torina, Antonio Cordaro, Valeria Blanda, Rosalia D'Agostino, Salvatore Scimeca, Maria E Scariano, Guido Sireci, Rossella Lelli
Babesiosis due to Babesia bigemina is a relevant tick-borne disease, affecting cattle worldwide. Many surface proteins of the pathogen including the Apical Membrane Antigen 1 (AMA-1) - have been analysed for vaccine and diagnostic purposes. This study focused on B. bigemina AMA-1 and on its use for the assessment of diagnostic tests. After bioinformatic analyses, AMA-1 codifying region was amplified and cloned into an expression vector used to induce protein synthesis in Escherichia coli cells. AMA-1 was purified by affinity chromatography and used to set up the best condition for an ELISA protocol...
January 2016: Veterinaria Italiana
Rafiou Adamou, Francine Chénou, Ibrahim Sadissou, Paulin Sonon, Célia Dechavanne, Abdelkader Djilali-Saïah, Gilles Cottrell, Agnès Le Port, Achille Massougbodji, Edmond J Remarque, Adrian J F Luty, Ambaliou Sanni, André Garcia, Florence Migot-Nabias, Jacqueline Milet, David Courtin
Antibodies that impede the invasion of Plasmodium falciparum (P. falciparum) merozoites into erythrocytes play a critical role in anti-malarial immunity. The Growth Inhibition Assay (GIA) is an in vitro measure of the functional capacity of such antibodies to limit erythrocyte invasion and/or parasite growth. Up to now, it is unclear whether growth-inhibitory activity correlates with protection from clinical disease and there are inconsistent results from studies performed with GIA. Studies that have focused on the relationship between IgGs and their in vitro parasite Growth Inhibition Activity (GIAc) in infants aged less than two years old are rare...
July 2016: Acta Tropica
Yan Shen, Jun Wang, Yuxiao Huang, Jiao Liang, Xuewu Liu, Dudu Wu, He Jiang, Ya Zhao, Yinghui Li
Malaria is a severe, life-threatening infectious disease that endangers human health. However, there are no vaccines or immune strategy of vaccines succeeding in both erythrocytic and pre-erythrocytic stage. During the liver stage of the Plasmodium life cycle, sporozoites invade the host liver cells. The sporozoites, then, induce a cellular immune response via the major histocompatibility complex (MHC) molecules on their surfaces. The cytotoxic T lymphocytes (CTLs) then recognize the corresponding antigen-MHC complex on the surfaces of these infected liver cells and kill them...
May 2016: Parasitology Research
Maryam Salavatifar, Sedigheh Zakeri, Akram Abouie Mehrizi, Sedigheh Mirkhazemi, Navid Dinparast Djadid
BACKGROUND: Acquired antibody responses following natural infection provide valuable information for selection of candidate antigens for malaria vaccines. Apical membrane antigen-1 of Plasmodium vivax (PvAMA-1) has potential as a component of a subunit vaccine for vivax malaria. In addition, genetic diversity in this antigen is responsible for challenges in the development of an effective PvAMA-1 based vaccine. Therefore, the main aim of this study was to determine whether allelic polymorphisms in pvama-1 influence the recognition of naturally occurring antibodies...
December 2015: Archives of Iranian Medicine
Juan Camilo Sánchez-Arcila, Marcelle Marcolino de França, Virginia Araujo Pereira, Mariana Pinheiro Alves Vasconcelos, Antonio Têva, Daiana de Souza Perce-da-Silva, Joffre Rezende Neto, Cesarino Junior Lima Aprígio, Josue da Costa Lima-Junior, Mauricio Martins Rodrigues, Irene Silva Soares, Dalma Maria Banic, Joseli Oliveira-Ferreira
BACKGROUND: Polyparasitism is a common condition in humans but its impact on the host immune system and clinical diseases is still poorly understood. There are few studies of the prevalence and the effect of malaria-intestinal parasite co-infections in the immune response to malaria vaccine candidates. The present study determines whether the presence of malaria and intestinal parasites co-infection is associated with impaired IgG responses to Plasmodium vivax AMA-1 and MSP-119 in a rural population of the Brazilian Amazon...
2015: Malaria Journal
Maryam Salavatifar, Sedigheh Zakeri, Nasim Hayati Roodbari, Navid Dinparast Djadid
OBJECTIVE: The apical membrane antigen-1 (AMA-1) is considered as a promising candidate for development of a malaria vaccine against Plasmodium parasites. The correct conformation of this protein appears to be necessary for the stimulation of parasite-inhibitory responses, and these responses, in turn, seem to be antibody-mediated. Therefore, in the present investigation, we expressed the Plasmodium vivax AMA-1 (PvAMA-1) ectodomain in Escherichia coli (E. coli), purified it using standard procedures and characterized it to determine its biological activities for it to be used as a potential target for developing a protective and safe vivax malaria vaccine...
2015: Cell Journal
Mun Yik Fong, Shen Siang Wong, Jeremy Ryan De Silva, Yee Ling Lau
The simian malaria parasite Plasmodium knowlesi is now recognized as a species that can cause human malaria. The first report of large scale human knowlesi malaria was in 2004 in Malaysia Borneo. Since then, hundreds of human knowlesi malaria cases have been reported in Southeast Asia. The present study investigates the genetic polymorphism of P. knowlesi DI domain of the apical membrane antigen-1 (AMA-1), a protein considered as a promising vaccine candidate for malaria. The DI domain of AMA-1 gene of P. knowlesi clinical isolates from Peninsular Malaysia was amplified by PCR, cloned into Escherichia coli, then sequenced and analysed...
December 2015: Acta Tropica
M A Someabozorg, S Mirkazemi, A A Mehrizi, F Shokri, N D Djadid, S Zakeri
Naloxone (NLX) has ability to shift the immune response to a Th1 profile. Therefore, the adjuvant efficacy of NLX with recombinant P. vivax apical membrane antigen-1(rPvAMA-1) in BALB/c mice was evaluated. Mice were immunized subcutaneously with purified rPvAMA-1 formulated with NLX (doses of 5 mg/kg body weight) alone or in combination with IFA. A significant increase in anti-PvAMA-1 IgG antibody after the second boost (mean OD490 = 2.08 and 2.17, in groups received, rPvAMA-1/NLX and rPvAMA-1/-NLX/IFA, respectively) was detected...
August 1, 2015: Parasite Immunology
E Moreau, C Bonsergent, I Al Dybiat, L M Gonzalez, C A Lobo, E Montero, L Malandrin
Babesiosis is an important veterinary and zoonotic tick borne disease caused by the hemoprotozoan Babesia spp. which infects red blood cell of its vertebrate host. In order to control the infection, vaccination that targets molecules involved in the invasion process of red blood cells could provide a good alternative to chemotherapy. Among these molecules, Apical Membrane Antigen-1 (AMA-1) has been described as an excellent vaccine candidate in Plasmodium spp. In this paper, we have investigated AMA-1 of Babesia divergens (BdAMA-1) as vaccine candidate by evaluating its polymorphism and by studying the humoral response against BdAMA-1 of sheep experimentally infected with B...
August 2015: Experimental Parasitology
Franciele Maira Moreira Batista Tomaz, Adriana Antônia da Cruz Furini, Marcela Petrolini Capobianco, Marinete Marins Póvoa, Pamella Cristina Alves Trindade, Valéria Daltibari Fraga, Luciana Moran Conceição, Lucas Ribeiro de Azevedo, Sônia Maria Oliani, Gustavo Capatti Cassiano, Carlos Eugênio Cavasini, Sidney Emanuel Batista Dos Santos, Ricardo Luiz Dantas Machado
BACKGROUND: Several studies have recently demonstrated that the immune responses against malaria is governed by different factors, including the genetic components of the host. The IL-4 gene appears to be a strong candidate factor because of its role in the regulation of the Th2 response. The present study investigated the role of IL-4 polymorphisms in the development of IgG antibodies against PvAMA-1 and the IL-4 levels in individuals infected with Plasmodium vivax in a malaria endemic area in the Brazilian Amazon...
August 2015: Cytokine
Ahmad Reza Esmaeili Rastaghi, Fatemeh Nedaei, Hossein Nahrevanian, Nazanin Hoseinkhan
Apical membrane antigen-1 (AMA-1) of Plasmodium vivax Grassi et Feletti, 1890 is a promising malaria vaccine candidate. However, antigenic variation is a major problem to design a universal malaria vaccine. Hence, detailed understanding of the pvama-1 gene polymorphism can provide conductive information on this potential vaccine component. Therefore, this study investigated the extent of genetic polymorphisms at domain I (DI), DII and partial DIII of AMA-1 among Iranian P. vivax isolates. Out of 107 blood samples, 92 were analysed based on the quality of the sequencing data...
October 2014: Folia Parasitologica
Aliehsan Heidari, Hossein Keshavarz, Homa Hajjaran, Seyyed Meisam Ebrahimi, Kourosh Kabir, Mohammad Hassan Naseri
BACKGROUND: Apical Membrane antigen 1 (AMA-1) is positioned on the surface of merozoite and it may play a role in attack to red blood cells. The main aim of present study was to determine the genetic variation, as well as, to detect of selection at domain I of AMA-1 gene Plasmodium vivax isolates in Iran. METHODS: Blood samples were collected from 58 patients positive for P. vivax, mono infection and the domain I of AMA-1 gene was amplified by nested PCR and then sequenced...
October 2013: Iranian Journal of Parasitology
Michael E von Fricken, Thomas A Weppelmann, Brandon Lam, Will T Eaton, Laura Schick, Roseline Masse, Madsen V Beau De Rochars, Alexandre Existe, Joseph Larkin, Bernard A Okech
BACKGROUND: Malaria transmission continues to occur in Haiti, with 25,423 confirmed cases of Plasmodium falciparum and 161,236 suspected infections reported in 2012. At low prevalence levels, passive surveillance measures, which rely primarily on reports from health systems, becomes less appropriate for capturing annual malaria incidence. To improve understanding of malaria transmission in Haiti, participants from the Ouest and Sud-Est departments were screened using a highly sensitive enzyme-linked immunosorbent assay (ELISA)...
2014: Malaria Journal
Elaine C Vicentin, Kátia S Françoso, Mariana V Rocha, Dmitri Iourtov, Fernanda L Dos Santos, Flávia S Kubrusly, Maria A Sakauchi, Isaias Raw, Francois Nosten, Laurent Rénia, Mauricio M Rodrigues, Bruce Russell, Irene S Soares
In a recent vaccine trial performed with African children, immunization with a recombinant protein based on Plasmodium falciparum apical membrane antigen 1 (AMA-1) conferred a significant degree of strain-specific resistance against malaria. To contribute to the efforts of generating a vaccine against Plasmodium vivax malaria, we expressed the ectodomain of P. vivax AMA-1 (PvAMA-1) as a secreted soluble protein in the methylotrophic yeast Pichia pastoris. Recognized by a high percentage of sera from individuals infected by P...
March 2014: Infection and Immunity
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