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https://www.readbyqxmd.com/read/29224159/quantitation-of-dna-methylation-by-quantitative-multiplex-methylation-specific-pcr-qm-msp-assay
#1
Mary Jo Fackler, Saraswati Sukumar
The defining feature of the Quantitative Multiplex Methylation-Specific PCR (QM-MSP) method to sensitively quantify DNA methylation is the two-step PCR approach for a multiplexed analysis of a panel of up to 12 genes in clinical samples with minimal quantities of DNA. In the first step, for up to 12 genes tested, one pair of gene-specific primers (forward and reverse) amplifies the methylated and unmethylated copies of the same gene simultaneously and in multiplex, in one PCR reaction. This methylation-independent amplification step produces amplicons of up to 109 copies per μL after 36 cycles of PCR...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29220586/co-occurring-psychosocial-problems-and-multiple-sexual-partners-among-men-who-have-sex-with-men-in-shanghai-china-a-syndemic-approach
#2
Zezhou Wang, Xu Zhao, Zhan Zhang, Mengyun Luo, Qiuming Shen, Yuanyuan Dong, Ying Wang, Yong Cai
We aimed to apply syndemic theory to verify the association and reinforcing effects between psychosocial problems and multiple sexual partners (MSP) of men who have sex with men (MSM) in Shanghai, China. A cross-sectional study was conducted among 547 MSM in Shanghai, China using a face-to-face survey. Sociodemographic and psychosocial data were collected, as well as information on MSP and condomless anal sex. In the 6 months prior to the survey, 54.3% of the MSM sample had had MSP. Univariate analysis showed that self-esteem, depression, loneliness, sexual compulsivity, and involuntary subordination (IS) were associated with MSP...
December 8, 2017: Journal of Sex Research
https://www.readbyqxmd.com/read/29205508/aberrant-methylation-of-mutl-homolog-1-is-associated-with-increased-risk-of-non-small-cell-lung-cancer
#3
Haochang Hu, Xiaoying Chen, Cong Zhou, Bin Li, Yong Yang, Xiuru Ying, Yiyi Mao, Yihan Zhang, Jie Zhong, Jie Dai, Hang Yu, Boyi Wu, Xiaodong Li, Tiangong Wang, Shiwei Duan
BACKGROUND: Non-small cell lung cancer (NSCLC) is a common malignant tumor. DNA hypermethylation in the promoter region has been served as a potential molecular marker for several tumors. The goal of the current study was to assess the diagnostic ability of mutL homolog 1 (MLH1) promoter methylation in NSCLC. METHODS: A total of 111 NSCLC patients' paired tissue samples were obtained to explore the association between MLH1 promoter methylation and NSCLC by methylation-specific polymerase chain reaction (MSP) method...
December 5, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29202805/clinical-significance-of-pcdh10-promoter-methylation-in-diffuse-large-b-cell-lymphoma
#4
Wenting Huang, Xuemin Xue, Ling Shan, Tian Qiu, Lei Guo, Jianming Ying, Ning Lu
BACKGROUND: PCDH10, one of the non-clustered protocadherins, is identified as a tumor suppressor gene in many tumors. Recently, promoter methylation of PCDH10 was found in diffuse large B-cell lymphoma (DLBCL) but not in normal lymph nodes, suggesting that its epigenetic aberrance is essential to the lymphomagenesis. However, there are few studies on the clinicopathological relevance and prognostic significance of PCDH10 methylation status in DLBCL. METHODS: One hundred-seven cases of DLBCL between Jan 2009 and Jul 2010 were selected to extract genomic DNA and perform bisulfite modification...
December 4, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29197086/methylation-of-oprl1-mediates-the-effect-of-psychosocial-stress-on-binge-drinking-in-adolescents
#5
Barbara Ruggeri, Christine Macare, Serena Stopponi, Tianye Jia, Fabiana M Carvalho, Gabriel Robert, Tobias Banaschewski, Arun L W Bokde, Uli Bromberg, Christian Büchel, Anna Cattrell, Patricia J Conrod, Sylvane Desrivières, Herta Flor, Vincent Frouin, Jürgen Gallinat, Hugh Garavan, Penny Gowland, Andreas Heinz, Bernd Ittermann, Jean Luc Martinot, Marie-Laure Paillère Martinot, Frauke Nees, Dimitri Papadopoulos-Orfanos, Tomáš Paus, Luise Poustka, Michael N Smolka, Nora C Vetter, Henrik Walter, Robert Whelan, Wolfgang H Sommer, Georgy Bakalkin, Roberto Ciccocioppo, Gunter Schumann
BACKGROUND: Nociceptin is a key regulator linking environmental stress and alcohol drinking. In a genome-wide methylation analysis, we recently identified an association of a methylated region in the OPRL1 gene with alcohol-use disorders. METHODS: Here, we investigate the biological basis of this observation by analysing psychosocial stressors, methylation of the OPRL1 gene, brain response during reward anticipation and alcohol drinking in 660 fourteen-year-old adolescents of the IMAGEN study...
December 2, 2017: Journal of Child Psychology and Psychiatry, and Allied Disciplines
https://www.readbyqxmd.com/read/29178003/enhanced-phosphorus-removal-using-acid-treated-magnesium-slag-particles
#6
Xianqiang Tang, Rui Li, Min Wu, Lei Dong, Zhenhua Wang
Magnesium-enriched magnesium slag particles (MSPs) can be used as an adsorption substrate as well as the magnesium source for struvite precipitation. In this study, an HCl treatment was used to enhance MSPs for phosphorus removal. After soaking in 1 mol/L HCl, an 11.27% decrease in median diameter (D50) and a 6.73% increase in specific surface area were observed when compared with the original MSPs. The improvement of the MSP surface properties resulted in 188.96 mg/kg increase in the PO43- adsorption capacity...
November 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29161283/prognostic-significance-of-promoter-dna-hypermethylation-of-the-cysteine-dioxygenase-1-cdo1-gene-in-primary-gallbladder-cancer-and-gallbladder-disease
#7
Kazuharu Igarashi, Keishi Yamashita, Hiroshi Katoh, Keita Kojima, Yosuke Ooizumi, Nobuyuki Nishizawa, Ryo Nishiyama, Hiroshi Kawamata, Hiroshi Tajima, Takashi Kaizu, Yusuke Kumamoto, Masahiko Watanabe
BACKGROUND: Aberrant promoter DNA methylation of the cysteine dioxygenase 1 (CDO1) gene is found in various human cancers and is associated with clinical outcome. In this study, we assessed for the first time the clinicopathological significance of CDO1 methylation in primary gallbladder cancer (GBC) in comparison with non-malignant gallbladder disease. METHODS: CDO1 DNA methylation was quantified using quantitative TaqMan methylation specific PCR (Q-MSP) in 99 primary GBC patients together with the 78 corresponding non-tumor tissues and 26 benign gallbladder disease (including 7 patients with xanthogranulomatous cholecystitis) who underwent surgical resection between 1986 and 2014...
2017: PloS One
https://www.readbyqxmd.com/read/29150948/methylation-associated-dok1-and-dok2-down-regulation-potential-biomarkers-for-predicting-adverse-prognosis-in-acute-myeloid-leukemia
#8
Pin-Fang He, Zi-Jun Xu, Jing-Dong Zhou, Xi-Xi Li, Wei Zhang, De-Hong Wu, Zhi-Hui Zhang, Xin-Yue Lian, Xin-Yu Yao, Zhao-Qun Deng, Jiang Lin, Jun Qian
DOK-1 and DOK-2 (DOK1/2) are closely related members of downstream of tyrosine kinase (DOK) family genes, which are found to be frequently rearranged in several hematopoietic cancers. However, the clinical implications of DOK1/2 in acute myeloid leukemia (AML) remain largely unknown. To investigate the clinical significance, real-time quantitative PCR (RQ-PCR) was carried out to detect DOK1/2 expressions in 125 de novo AML patients and 28 healthy controls. Real-time quantitative methylation-specific PCR (RQ-MSP) and bisulfite sequencing PCR (BSP) were applied to detect DOK1/2 methylation level and density...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29145503/zinc-sulfate-contributes-to-promote-telomere-length-extension-via-increasing-telomerase-gene-expression-telomerase-activity-and-change-in-the-tert-gene-promoter-cpg-island-methylation-status-of-human-adipose-derived-mesenchymal-stem-cells
#9
Raheleh Farahzadi, Ezzatollah Fathi, Seyed Alireza Mesbah-Namin, Nosratollah Zarghami
The use of mesenchymal stem cells (MSCs) for cell therapy and regenerative medicine has received widespread attention over the past few years, but their application can be complicated by factors such as reduction in proliferation potential, the senescent tendency of the MSCs upon expansion and their age-dependent decline in number and function. It was shown that all the mentioned features were accompanied by a reduction in telomerase activity and telomere shortening. Furthermore, the role of epigenetic changes in aging, especially changes in promoter methylation, was reported...
2017: PloS One
https://www.readbyqxmd.com/read/29133429/microbial-pathway-for-anaerobic-5-methylthioadenosine-metabolism-coupled-to-ethylene-formation
#10
Justin A North, Anthony R Miller, John A Wildenthal, Sarah J Young, F Robert Tabita
Numerous cellular processes involving S-adenosyl-l-methionine result in the formation of the toxic by-product, 5'-methylthioadenosine (MTA). To prevent inhibitory MTA accumulation and retain biologically available sulfur, most organisms possess the "universal" methionine salvage pathway (MSP). However, the universal MSP is inherently aerobic due to a requirement of molecular oxygen for one of the key enzymes. Here, we report the presence of an exclusively anaerobic MSP that couples MTA metabolism to ethylene formation in the phototrophic bacteria Rhodospirillum rubrum and Rhodopseudomonas palustris In vivo metabolite analysis of gene deletion strains demonstrated that this anaerobic MSP functions via sequential action of MTA phosphorylase (MtnP), 5-(methylthio)ribose-1-phosphate isomerase (MtnA), and an annotated class II aldolase-like protein (Ald2) to form 2-(methylthio)acetaldehyde as an intermediate...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29128865/promoter-methylation-of-rassf1a-indicates-prognosis-for-patients-with-stage-ii-and-iii-colorectal-cancer-treated-with-oxaliplatin-based-chemotherapy
#11
Xicai Sun, Wei Yuan, Furong Hao, Wenzhen Zhuang
BACKGROUND The purpose of this study was to investigate the prognostic significance of methylation of RAS association domain family protein 1 (RASSF1A) in the promoter region for patients with stage II and III colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy. MATERIAL AND METHODS There were 108 eligible CRC patients and 78 healthy controls included in this study. Methylation-specific polymerase chain reaction (MSP) was applied to detect the methylation status of RASSF1A in patients before and after chemotherapy...
November 12, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29121929/variation-in-the-immune-responses-against-plasmodium-falciparum-merozoite-surface-protein-1-and-apical-membrane-antigen-1-in-children-residing-in-the-different-epidemiological-strata-of-malaria-in-cameroon
#12
Tebit Emmanuel Kwenti, Adzemye Linus Moye, Adzemye Basil Wiylanyuy, Longdoh Anna Njunda, Theresa Nkuo-Akenji
BACKGROUND: Studies to assess the immune responses against malaria in Cameroonian children are limited. The purpose of this study was to assess the immune responses against Plasmodium falciparum merozoite surface protein-1 (MSP-119) and apical membrane antigen-1 (AMA-1) in children residing in the different epidemiological strata of malaria in Cameroon. METHODS: In a cross-sectional survey performed between April and July 2015, 602 children between 2 and 15 years (mean ± SD = 5...
November 9, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29113164/methylation-and-expression-of-reck-p53-and-runx-genes-in-patients-with-esophageal-cancer
#13
Yanrong Lu, Baerxiaguli Zabihula, Waresijiang Yibulayin, Xiang Liu
The methylation and expression of RECK, P53 and RUNX genes in patients with esophageal cancer was investigated. In order to achieve this aim, a sample of 58 patients with esophageal cancer, treated between February 2013 and February 2014, were considered as the observation group. Additionally, a sample of 42 healthy individuals was selected as the control group. Methylation status of RECK, P53 and RUNX genes from the observation and control groups were detected by MSP. Reverse transcriptase-quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), western blot and immunohistochemistry were used to detect the mRNA and protein levels of RECK, P53 and RUNX in both the observation and the control groups...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29100464/core-shell-gene-carriers-with-different-lengths-of-plga-chains-to-transfect-endothelial-cells
#14
Xinghong Duo, Qian Li, Jun Wang, Juan Lv, Xuefang Hao, Yakai Feng, Xiangkui Ren, Changcan Shi, Wencheng Zhang
In order to improve the transfection efficiency and reduce the cytotoxicity of gene carriers, many strategies have been used to develop novel gene carriers. In this study, five complex micelles (MSP(2 k), MSP(4 k), MSP(6 k), MSP(8 k), and MSP(10 k)) were prepared from methoxy-poly(ethylene glycol)-b-poly(d,l-lactide-co-glycolide) (mPEG-b-PLGA) and sorbitol-poly(d,l-lactide-co-glycolide)-graft-PEI (sorbitol-PLGA-g-PEI, where the designed molecular weights of PLGA chains were 2 kDa, 4 kDa, 6 kDa, 8 kDa, and 10 kDa, respectively) copolymers by a self-assembly method, and the mass ratio of mPEG-b-PLGA to sorbitol-PLGA-g-PEI was 1/3...
November 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29099287/pax5-gene-as-a-novel-methylation-marker-that-predicts-both-clinical-outcome-and-cisplatin-sensitivity-in-esophageal-squamous-cell-carcinoma
#15
Keisuke Kurimoto, Masamichi Hayashi, Rafael Guerrero-Preston, Masahiko Koike, Mitsuro Kanda, Sho Hirabayashi, Hiroshi Tanabe, Nao Takano, Naoki Iwata, Yukiko Niwa, Hideki Takami, Daisuke Kobayashi, Chie Tanaka, Suguru Yamada, Goro Nakayama, Hiroyuki Sugimoto, Tsutomu Fujii, Michitaka Fujiwara, Yasuhiro Kodera
Therapeutic strategies for esophageal cancer largely depend on histopathological assessment. To select appropriate treatments for individual patients, we examined the background molecular characteristics of tumor malignancy and sensitivity to multidisciplinary therapy. Seventy-eight surgically-resected esophageal squamous cell carcinoma (ESCC) cases during 2001-2013 were examined. PAX5, a novel gene methylation marker in ESCC, was evaluated in the specimens, as methylation of this gene was identified as an extremely tumor-specific event in squamous cell carcinogenesis of head and neck...
November 3, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/29082740/methylation-status-of-the-estrogen-receptor-1-promoter-predicts-poor-prognosis-of-acute-on-chronic-hepatitis-b-liver-failure
#16
Xiao-Peng Fan, Cheng-Yun Dou, Yu-Chen Fan, Chuang-Jie Cao, Ze-Hua Zhao, Kai Wang
BACKGROUND: Acute-on-chronic hepatitis B liver failure (ACHBLF) is an acute deteriorating liver disease and rapidly progresses to multiple organ failure. There is currently no adequate accurate predictive models of ACHBLF prognosis. AIMS: To identify the methylation frequency of the estrogen receptor 1 (ESR1) promoter in ACHBLF and analyze the associated prognostic significance. METHODS: Methylation-specific PCR (MSP) was used to determine the methylation frequency of the ESR1 promoter in peripheral blood mononuclear cells from a training and validation cohort of patients...
October 30, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/29075332/hypermethylation-in-the-promoter-of-the-mthfr-gene-is-associated-with-diabetic-complications-and-biochemical-indicators
#17
Mayara Karla Dos Santos Nunes, Alexandre Sérgio Silva, Isabella Wanderley de Queiroga Evangelista, João Modesto Filho, Cecília Neta Alves Pegado Gomes, Rayner Anderson Ferreira do Nascimento, Rafaella Cristhine Pordeus Luna, Maria José de Carvalho Costa, Naila Francis Paulo de Oliveira, Darlene Camati Persuhn
BACKGROUND: DNA methylation is an epigenetic mechanism for regulating the transcription of many genes and has been linked to the development of various diseases. A promising gene to investigate is methylenetetrahydrofolate reductase (MTHFR), since the enzyme methylenetetrahydrofolate reductase (MTHFR) promotes methyl radical synthesis in the homocysteine cycle and can provide methyl groups for DNA methylation. In addition, several studies have correlated gene polymorphisms of this enzyme with a greater risk of diabetes, but little is known regarding the relationship between epigenetic changes in this gene and diabetes and its complications...
2017: Diabetology & Metabolic Syndrome
https://www.readbyqxmd.com/read/29075061/harmine-a-novel-dna-methyltransferase-1-inhibitor-in-the-leukemia-cell-line
#18
Arezoo Oodi, Hamed Norouzi, Naser Amirizadeh, Mahin Nikougoftar, Zahra Vafaie
DNA methylation followed by tumor suppressor gene repression plays a critical role in the leukemia development. So, DNA methyl transferase inhibitors have great importance in treatment of theses malignancies. Harmine, A beta carboline alkaloid derivative of Peganum harmala, had shown anti- proliferative effects on leukemic cell line. This study aimed to evaluate the effect of Harmine on DNMT1 (DNA methyl transferase 1) expression in a leukemic cell line. Cell proliferation and cell cycle analysis were studied in NB4 cell line after treatment with Harmine for 72 h...
December 2017: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29071769/inhibition-of-fatty-acid-amide-hydrolase-in-the-central-amygdala-alleviates-co-morbid-expression-of-innate-anxiety-and-excessive-alcohol-intake
#19
Serena Stopponi, Yannick Fotio, Ana Domi, Anna Maria Borruto, Luis Natividad, Marisa Roberto, Roberto Ciccocioppo, Nazzareno Cannella
Fatty acid amide hydrolase (FAAH) is an enzyme that prominently degrades the major endocannabinoid N-arachidonoylethanolamine (anandamide). Inhibition of this enzyme leads to increased anandamide levels in brain regions that modulate stress and anxiety. Recently, we found that genetically selected Marchigian Sardinian alcohol-preferring (msP) rats display hyperactive FAAH in amygdalar regions that was associated with increased stress sensitivity and a hyper-anxious phenotype. Our previous work has also demonstrated that msPs display an innate preference for and excessive consumption of alcohol, potentially reflecting a form of self-medication to gain relief from hyper-anxious states...
October 26, 2017: Addiction Biology
https://www.readbyqxmd.com/read/29063888/epidemiology-of-malaria-and-msp-2-gene-based-genetic-diversity-of-plasmodium-falciparum-from-patients-attending-community-health-centre-jiribam-manipur
#20
Indu Sharma, Appu Saikia, Paromita Chakraborty
Two millilitres of peripheral blood was collected from 323 patients of different age groups and rapid diagnosis (RDT test) was performed. Parasite genomic DNA was extracted from whole blood and MSP-2 gene-based diversity and polymorphism was determined followed by restriction fragment length polymorphism analysis using Taq 1 and Vsp 1 digestion of each polymerase chain reaction product to analyse MSP-2 genotypes. Twenty-six sequences of P. falciparum MSP-2 gene were retrieved from the current GenBank database to represent strains from various geographical locations and were analysed for the Taq 1 and Vsp 1 enzyme restriction sites using in silico restriction digestion in Sequence Manipulation Suite, Version 2...
July 2017: Indian Journal of Medical Microbiology
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