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https://www.readbyqxmd.com/read/29670585/maldi-spectra-database-for-rapid-discrimination-and-subtyping-of-mycobacterium-kansasii
#1
Jayaseelan Murugaiyan, Astrid Lewin, Elisabeth Kamal, Zofia Bakuła, Jakko van Ingen, Vit Ulmann, Miren J Unzaga Barañano, Joanna Humięcka, Aleksandra Safianowska, Uwe H Roesler, Tomasz Jagielski
Mycobacterium kansasii is an emerging non-tuberculous mycobacterial (NTM) pathogen capable of causing severe lung disease. Of the seven currently recognized M. kansasii genotypes (I-VII), genotypes I and II are most prevalent and have been associated with human disease, whereas the other five (III-VII) genotypes are predominantly of environmental origin and are believed to be non-pathogenic. Subtyping of M. kansasii serves as a valuable tool to guide clinicians in pursuing diagnosis and to initiate the proper timely treatment...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29665905/-relationship-between-dnmt1-and-methylation-of-shp-1-promoter-2-in-k562-cells
#2
Xue-Dong Liu, Xiao Liu, Xiu-Fen Guo, Jian-Min Luo, Ying-Hua Li
OBJECTIVE: To investigate the relationship of DNA methyltransferase 1 ( DNMT1 ) with hematopoietic cell phosphatase (SHP-1) gene expression and promoter 2 methylation status in cell line K562. METHODS: The promoter sequence of SHP-1 gene promoter 2 in NCBI database was analyzed, the K562 cells were transfected with the lentiviral plasmids-the specified retroviral vector psiHIV-mU6-shDNMT1 and psiHIV-mU6-mcherryFP-control. The methylation status of SHP-1 gene promoter 2 in K562 cells was detected by methylation-specific polymerase chain reaction (MSP) and bisulfite-modified sequencing (BSP)...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29658966/methylation-in-the-promoter-regions-of-wt1-nkx6-1-and-dbc1-genes-in-cervical-cancer-tissues-of-uygur-women-in-xinjiang
#3
Dan Wu, Jinli Zhang, Peiwen Fan, Hongtao Li, Dongmei Li, Huan Pan, Hongchang He, Xianxian Ren, Zhenzhen Pan, Renfu Shao, Zemin Pan
This study aimed to explore: 1) DNA methylation in the promoter regions of Wilms tumor gene 1 (WT1), NK6 transcription factor related locus 1 gene (NKX6-1) and Deleted in bladder cancer 1 (DBC1) gene in cervical cancer tissues of Uygur women in Xinjiang, and 2) the correlation of gene methylation with the infection of HPV16/18 viruses. We detected HPV16/18 infection in 43 normal cervical tissues, 30 cervical intraepithelial neoplasia lesions (CIN) and 48 cervical cancer tissues with polymerase chain reaction (PCR) method...
January 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29626433/plasmodium-falciparum-merozoite-protein-1-genetic-diversity-and-multiplicity-of-infection-in-isolates-from-congolese-children-consulting-in-a-pediatric-hospital-in-brazzaville
#4
Nerly Shirère Gampio Gueye, Francine Ntoumi, Christevy Vouvoungui, Simon Charles Kobawila, Michael N Kombo, Alain Mouanga, Julia Deibert, Felix Koukouikila-Koussounda
As in many sub-Saharan African countries, the burden of malaria has been reduced in the Republic of Congo as a result of massive deployment of insecticide treated nets and availability of artemisinin-combinations therapies (ACTs). High to moderate genetic diversity of msp-1 gene of Plasmodium falciparum (P. falciparum) has been reported from different parts of the world but limited data are available from Central Africa including the Republic of Congo. For this reason, the aim of study was to investigate the P...
April 4, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29574515/a-regional-algorithm-to-model-mesozooplankton-biomass-along-the-southwestern-bay-of-bengal
#5
R Mahesh, A Saravanakumar, T Thangaradjou, H U Solanki, Mini Raman
A three-dimensional regression analysis attempted to model mesozooplankton (MSP) biomass using sea surface temperature (SST) and chlorophyll-a (Chl-a). The study was carried out from January 2014 to July 2015 in the southwestern Bay of Bengal (BoB) and sampling was carried out on board Sagar Manjusha and Sagar Purvi. SST ranged from 26.2 to 33.1 °C while Chl-a varied from 0.04 to 6.09 μg L-1 . During the course of the study period, there was a weak correlation (r = 0.32) between SST and Chl-a statistically...
March 24, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29562865/regulatable-lentiviral-hematopoietic-stem-cell-gene-therapy-in-a-mouse-model-of-parkinson-s-disease
#6
Guo Ge, Cang Chen, Michael James Guderyon, Jingwei Liu, Zhixu He, Yanni Yu, Robert A Clark, Senlin Li
Glial cell line-derived neurotrophic factor (GDNF) exhibits potent neuroprotective properties in preclinical models of Parkinson's disease (PD), but challenges in GDNF delivery have been reported from clinical trials. To address this barrier, we developed a hematopoietic stem cell transplantation (HSCT) -based macrophage-mediated GDNF therapy platform. Here we introduced a regulatable lentiviral vector (LV-MSP-Tet-Off-hGDNF) in order to allow the expression of human GDNF to be adjusted or stopped by oral administration of doxycycline (Dox)...
March 21, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29557690/hypomethylation-of-the-mirna-34a-gene-promoter-is-associated-with-severe-preeclampsia
#7
Mahnaz Rezaei, Fatemeh Eskandari, Abbas Mohammadpour-Gharehbagh, Mahdiyeh Harati-Sadegh, Batool Teimoori, Saeedeh Salimi
PURPOSE: PE is a pregnancy-specific complication, which genetic and epigenetic factors play key roles in its pathogenesis. DNA methylation is a main epigenetic alteration with important roles in gene regulation. Micro RNAs (miRNAs) as another member of epigenetic machinery regulate the gene expression and involve in different biological pathways including apoptosis and placental development. Therefore, the present study performed to assess the association between miRNA-34a promoter methylation and PE susceptibility...
March 20, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29554659/clinical-significance-of-retinoic-acid-receptor-beta-promoter-methylation-in-prostate-cancer-a-meta-analysis
#8
MengMeng Dou, XueLiang Zhou, ZhiRui Fan, XianFei Ding, LiFeng Li, ShuLing Wang, Wenhua Xue, Hui Wang, Zhenhe Suo, XiaoMing Deng
BACKGROUND/AIMS: Retinoic acid receptor beta (RAR beta) is a retinoic acid receptor gene that has been shown to play key roles during multiple cancer processes, including cell proliferation, apoptosis, migration and invasion. Numerous studies have found that methylation of the RAR beta promoter contributed to the occurrence and development of malignant tumors. However, the connection between RAR beta promoter methylation and prostate cancer (PCa) remains unknown. This meta-analysis evaluated the clinical significance of RAR beta promoter methylation in PCa...
March 15, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29549022/landmark-independent-method-to-determine-midsagittal-plane-and-its-clinical-application-in-craniomaxillofacial-trauma
#9
Xiaotian Lian, Jun Guo, Wei Zeng, Hai Qing, Rui Ju, Wei Tang
PURPOSE: The purpose of this study was to explore new landmark-independent methods suitable for determining the midsagittal plane (MSP) in patients with craniomaxillofacial trauma and its clinical application. MATERIALS AND METHODS: Preoperative computed tomography data of 25 patients with craniomaxillofacial trauma were imported into Mimics software (Materialise, Leuven, Belgium) to reconstruct 3-dimensional skull models. In the experimental group, the MSP was determined by different landmarks that were clear, were scattered, and did not have displacement according to the types of fractures...
February 20, 2018: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29545906/the-clinical-influence-of-autophagy-associated-proteins-on-human-lung-cancer
#10
Yuan Chen, Kai Leonie Schnitzler, Yunxia Ma, Miljana Nenkov, Bernhard Theis, Iver Petersen
Exploitation of autophagy might potentially improve therapeutic strategy. Here, we analyzed the protein expression of autophagy-associated genes including LC3A, LC3B, Beclin-1, p62, and Atg5 in 88-131 primary lung tumors by immunohistochemistry (IHC) on tissue-microarrays (TMAs). Additionally, the DNA methylation pattern of LC3A was investigated by bisulfite sequencing (BS) and methylation-specific-PCR (MSP). It turned out that the higher expression of LC3A protein was associated with adenocarcinoma compared to squamous cell carcinoma of lung ( p = 0...
2018: Disease Markers
https://www.readbyqxmd.com/read/29514939/improved-discrimination-of-bacillus-anthracis-from-closely-related-species-in-the-bacillus-cereus-sensu-lato-group-based-on-maldi-tof-mass-spectrometry
#11
Viktoria I Pauker, Bryan R Thoma, Gregor Grass, Pauline Bleichert, Matthias Hanczaruk, Lothar Zöller, Sabine Zange
Discrimination of highly pathogenic bacteria, such as Bacillus anthracis , from closely related species based on molecular biological methods is challenging. We applied Matrix Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (MALDI-TOF MS) to a collection of B. anthracis strains and close relatives in order to significantly improve the statistical confidence of identification results for this group of bacteria. Protein mass spectra of 189 verified and diverse Bacillus strains of the Bacillus cereus sensu lato group were generated using MALDI-TOF MS and subsequently analyzed with supervised and unsupervised statistical methods, such as shrinkage discriminant analysis (SDA) and principle component analysis (PCA)...
March 7, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29511044/protein-protein-interaction-studies-reveal-the-plasmodium-falciparum-merozoite-surface-protein-1-region-involved-in-complex-formation-that-binds-to-human-erythrocytes
#12
Gourab Paul, Arunaditya Deshmukh, Bishwanath Kumar Chourasia, Md Kalamuddin, Ashutosh Panda, Susheel Kumar Singh, Puneet K Gupta, Asif Mohmmed, Virander S Chauhan, Michael Theisen, Pawan Malhotra
Plasmodium  falciparum Merozoite surface protein (MSP) 1 has been studied extensively as a vaccine candidate antigen. PfMSP-1 undergoes proteolytic processing into four major products; p83, p30, p38 and p42 that are associated in the form of non-covalent complex(s) with other merozoite surface proteins. To delineate MSP1 regions involved in the interaction with other MSPs, here we expressed recombinant proteins (PfMSP-165 ) encompassing part of p38 and p42 regions and PfMSP-119 PfMSP-165   interacted strongly with PfMSP-3, PfMSP-6, PfMSP-7, PfMSP-9, whereas PfMSP-119 did not interact with any of these proteins...
March 6, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29497387/evaluation-of-alcohol-preference-and-drinking-in-msp-rats-bearing-a-crhr1-promoter-polymorphism
#13
Marian L Logrip, John R Walker, Lydia O Ayanwuyi, Valentina Sabino, Roberto Ciccocioppo, George F Koob, Eric P Zorrilla
Alcoholism is a pervasive societal problem, yet available pharmacotherapies fail to treat most sufferers. The type 1 corticotropin-releasing factor (CRF1 ) receptor has received much attention for its putative role in the progression to alcohol dependence, although at present its success in clinical trials has been limited. Two single-nucleotide polymorphisms in the rat Crhr1 promoter have been identified in the Marchigian substrain of Sardinian alcohol-preferring (msP) rats. Unlike other Wistar-derived alcohol-preferring lines, nondependent msP rats reduce their alcohol self-administration in response to CRF1 antagonists and show increased brain CRF1 expression...
2018: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/29491567/a-study-on-prevalence-and-molecular-characterization-of-trypanosomal-species-infecting-equines-in-lahore-region-pakistan
#14
Naveed Sabir, Zafar Iqbal Chaudhry, Asim Aslam, Khushi Muhammad, Muhammad Shahid, Abid Hussain, Shahzad Akbar Khan, Ishtiaq Ahmad
Trypanosomiasis is an important protozoal disease with a diverse range of susceptible host including human. In the current study, molecular characterization of prevalent species was done through a pan-trypanosome polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). A total of three hundred (n = 300) equines including horses, donkeys and mules (100 each) were randomly selected and the equine blood samples were subjected to screening for trypanosomes through microhaematocrit centrifuge technique (MHCT), conventional PCR, semi-nested PCR and RFLP...
March 2018: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29475367/mir-148b-3p-affects-the-pathogenesis-of-adjuvant-induced-arthritis-rats-through-the-direct-target-dnmt1
#15
Chenggui Miao, Hao Yu, Jun Chang, Guoxue Zhang, Guoliang Zhou, Chuanlei Zhao
Our previous study showed that up-regulated DNA methyltransferase-1 (DNMT1) played an important role in the hypermethylation modification of SFRP4 in adjuvant-induced arthritis (AIA) rats. This work focused on the role of disordered miR-148b-3p in RA pathology and its corresponding regulatory targets. The expression of miR-148b-3p and DNMT1, and the effect of miR-148b-3p on the DNMT1 expression were determined by real-time qPCR, western blotting and double luciferase reporter genes. The role of miR-148b-3p on the SFRP4 expression, the canonical Wnt signaling and the pathology of AIA rats was investigated using real-time qPCR, western blotting and methylation-specific PCR (MSP)...
February 23, 2018: Autoimmunity
https://www.readbyqxmd.com/read/29468541/using-methylation-specific-pcr-to-study-rb1-promoter-hypermethylation
#16
Thaís M McCormick, Maria Da Glória Da C Carvalho
It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29458365/temporal-changes-in-genetic-diversity-of-msp-1-msp-2-and-msp-3-in-plasmodium-falciparum-isolates-from-grande-comore-island-after-introduction-of-act
#17
Bo Huang, Fei Tuo, Yuan Liang, Wanting Wu, Guangchao Wu, Shiguang Huang, Qirun Zhong, Xin-Zhuan Su, Hongying Zhang, Mingqiang Li, Affane Bacar, Kamal Said Abdallah, Ahamada M S A Mliva, Qi Wang, Zhaoli Yang, Shaoqin Zheng, Qin Xu, Jianping Song, Changsheng Deng
BACKGROUND: Malaria is still one of the serious public health problems in Grande Comore Island, although the number of annual cases has been greatly reduced in recent years. A better understanding of malaria parasite population diversity and transmission dynamics is critical for assessing the effectiveness of malaria control measures. The objective of this study is to investigate temporal changes in genetic diversity of Plasmodium falciparum populations and multiplicity of infection (MOI) in Grande Comore 10 years after introduction of ACT...
February 20, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29449053/genetic-and-epigenetic-alterations-in-the-tumour-tumour-margins-and-normal-buccal-mucosa-of-patients-with-oral-cancer
#18
N Eljabo, N Nikolic, J Carkic, D Jelovac, M Lazarevic, N Tanic, J Milasin
Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers...
February 12, 2018: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29444078/multiplex-serology-for-impact-evaluation-of-bed-net-distribution-on-burden-of-lymphatic-filariasis-and-four-species-of-human-malaria-in-northern-mozambique
#19
Mateusz M Plucinski, Baltazar Candrinho, Geraldo Chambe, João Muchanga, Olinda Muguande, Graça Matsinhe, Guidion Mathe, Eric Rogier, Timothy Doyle, Rose Zulliger, James Colborn, Abu Saifodine, Patrick Lammie, Jeffrey W Priest
BACKGROUND: Universal coverage with long-lasting insecticidal nets (LLINs) is a primary control strategy against Plasmodium falciparum malaria. However, its impact on the three other main species of human malaria and lymphatic filariasis (LF), which share the same vectors in many co-endemic areas, is not as well characterized. The recent development of multiplex antibody detection provides the opportunity for simultaneous evaluation of the impact of control measures on the burden of multiple diseases...
February 14, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29398759/tubercular-mycobacterial-spindle-cell-pseudotumour-a-case-report
#20
Deba Prasad Dhibar, Kamal Kant Sahu, Surjit Singh, Amanjit Bal, Abhijit Chougale, Varun Dhir
Pseudotumour is a benign inflammatory lesion. Mycobacterial spindle cell pseudotumour (MSP) is a rare pseudotumour. It is a benign proliferation of spindle-shaped histiocytes containing acid-fast mycobacterium, commonly reported in immunocompromised patients. MSP is usually associated with mycobacterium avium complex (MAC). Here, we present the case of a 38-year-old gentleman with acquired immune deficiency syndrome (AIDS) who presented with low-grade fever for 1-month duration. Clinically, he had generalised lymphadenopathy...
January 2018: Iranian Journal of Medical Sciences
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