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Phuong Kim Truong, Thuan Duc Lao, Thuy Ai Huyen LE
BACKGROUND: Human papillomavirus (HPV) infection has been considered as main cause of cervical cancer. Recently, aberrant DNA methylation at tumor suppressor genes (TSGs), leading to inactivation, has also been an early epigenetic event and cofactor in cervical carcinogenesis. This study was performed to evaluate an association between the hypermethylation of p16(INK4α) gene's promoter and HPV exposure in non-invasive samples (liquid-based papanicolaous samples) in Vietnamese population...
September 2017: Iranian Journal of Public Health
Ying-Li Lin, Yan-Li Li, Jian-Guo Ma
BACKGROUND PCDH8 is a newly-discovered suppressor gene that is frequently inactivated by aberrant methylation in several human cancers, including prostate cancer. The identification of PCDH8 methylation can be used as a potential predictive biomarker. Prostate cancer patients with high Gleason score are considered as being at high risk for tumor recurrence and progression, and adjuvant therapy is often routinely performed in clinical practice. In the present study, we did not measure the methylation of PCDH8 in these patients...
October 13, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Aleksandra Majchrzak-Celińska, Katarzyna Kosicka, Julia Paczkowska, Franciszek K Główka, Grzegorz H Bręborowicz, Mariola Krzyścin, Anna Siemiątkowska, Marcin Szaumkessel, Wanda Baer-Dubowska
Hypertensive Disorders of Pregnancy (HDsP) remain leading causes of maternal and perinatal morbidity and mortality. Growing evidence suggests the involvement of epigenetic factors, such as gene-specific and global DNA methylation changes, both in the etiology and as an effect of HDsP. In this study, we investigated the potential association between placental DNA methylation status in selected CpGs of HSD11B2 cortisol level controlling gene, RUNX3 tumor suppressor gene, and long interspersed nucleotide element-1 (LINE-1) repetitive elements and HDsP-preeclampsia (PE), gestational hypertension (GH), and chronic hypertension (CH)...
November 2017: Reproductive Sciences
Lilian Rose Pratt-Riccio, Daiana De Souza Perce-Da-Silva, Josué Da Costa Lima-Junior, Evelyn Kety Pratt Riccio, Marcelo Ribeiro-Alves, Fátima Santos, Mércia Arruda, Daniel Camus, Pierre Druilhe, Joseli Oliveira-Ferreira, Cláudio Tadeu Daniel-Ribeiro, Dalma Maria Banic
Peptide vaccine strategies using Plasmodium-derived antigens have emerged as an attractive approach against malaria. However, relatively few studies have been conducted with malaria-exposed populations from non-African countries. Herein, the seroepidemiological profile against Plasmodium falciparum of naturally exposed individuals from a Brazilian malaria-endemic area against synthetic peptides derived from vaccine candidates circumsporozoite protein (CSP), liver stage antigen-1 (LSA-1), erythrocyte binding antigen-175 (EBA-175), and merozoite surface protein-3 (MSP-3) was investigated...
September 18, 2017: American Journal of Tropical Medicine and Hygiene
Shahrbano Rostami, Fatemeh Nadali, Reza Alibakhshi, Farhad Zaker, Nahid Nasiri, Mehrdad Payandeh, Bahram Chahardouli, Ali Maleki
Background: Acute myeloid leukemia (AML) is a heterogeneous clonal disorder characterized by immature myeloid cell proliferation and bone marrow failure. Various genetic and epigenetic factors have been found to be influential in such patients. Methylation silencing of APAF-1, a putative tumor suppressor gene (TSG), has been found in several human malignancies. In this study, we explored the association of APAF-1 methylation status with AML patients. Materials and Methods: We studied the methylation status of APAF-1 gene in 101 AML patients and 50 healthy subjects as controls...
July 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
James Blain Johnston, Joss N Reimer, John L Wylie, Jared Bullard
OBJECTIVES: HIV point-of-care testing (POCT) has been available in Manitoba since 2008. This study evaluated the effectiveness of POCT at identifying individuals with previously unknown HIV status, its effects on clinical outcomes and the characteristics of the populations reached. METHODS: A retrospective database review was conducted for individuals who received HIV POCT from 2011 to 2014. Time to linkage to care and viral load suppression were compared between individuals who tested positive for HIV using POCT and controls identified as positive through standard screening...
October 7, 2017: Sexually Transmitted Infections
Carmen H Logie, Candice Lys, Moses Okumu, Cristina Leone
OBJECTIVES: Sexual and mental health disparities exist in the Northwest Territories (NWT) compared with other Canadian regions. STI rates are 10-fold higher, and youth suicide rates double the Canadian average. Scant research has examined associations between mental and sexual health among youth in the NWT. The study objective was to explore pathways from depression to multiple sex partners (MSP) among young women in the NWT, Canada. METHODS: We implemented a cross-sectional survey in 2015-2016 with a venue-based recruitment sample of young women aged 13-17 attending secondary schools in 17 NWT communities...
October 7, 2017: Sexually Transmitted Infections
Azadeh Ashtarinezhad, Ataollah Panahyab, Baharak Mohamadzadehasl, Farshad H Shirazi
Azole agents especially Miconazole are widely used even during pregnancy as antifungal agents. The aim of this study was to assess the usefulness of FTIR Micro-Spectroscopy for discriminating of Miconazole treated liver tissue from control liver tissue. The mice were injected with Miconazole (60 mg/Kg) on gestation day 9 and they were dissected on pregnancy day 15. The fetus fixed, dehydrated, and embedded in paraffin. Sections of liver (10 μM) were prepared from control and treated fetus groups by Microtome and deparaffinized with xylene...
2017: Iranian Journal of Pharmaceutical Research: IJPR
Yingjie Zhang, Huibo Li, Rongyi Cao, Lili Sun, Yan Wang, Shengjin Fan, Yanqiu Zhao, Desheng Kong, Lin Cui, Leilei Lin, Ke Wang, Yinghua Li, Jin Zhou
Inactivation of Dickkopf-3 (DKK3) is closely associated with a poor prognosis in various solid tumor and hematologic malignancies. Promoter hypermethylation is one potential cause of DKK3 inactivation. However, whether other mechanisms lead to DKK3 inactivation and the subsequent effects of these inactivations on cell proliferation and the Wnt signaling pathway in adult B acute lymphoblastic leukemia (B-ALL) remain unclear. In the present study, we found that low DKK3 expression levels were associated with high miR-708 expression and promoter hypermethylation in adult B-ALL...
September 8, 2017: Oncotarget
Ramona Faas, Annelie Pohle, Karin Moß, Marius Henkel, Rudolf Hausmann
Nanodiscs are membrane mimetics which may be used as tools for biochemical and biophysical studies of a variety of membrane proteins. These nanoscale structures are composed of a phospholipid bilayer held together by an amphipathic membrane scaffold protein (MSP). In the past, nanodiscs were successfully assembled with membrane scaffold protein 1D1 and 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine with a homogeneous diameter of ∼10 nm. In this study, the formation of nanoscale particles from MSP1D1 and rhamnolipid biosurfactants is investigated...
December 2017: Biotechnology Reports
Liang Zhou, Ningfu Wang, Hong Li, Guoxin Tong, Jianmin Yang, Lei Lai, Hao Pan, Xianhua Ye, Jinyu Huang
BACKGROUND: Inflammation is involved in the development of In-stent restenosis (ISR) after percutaneous coronary intervention. We aimed to investigate the association between of suppressor of cytokine signaling-1 (SOCS1), a major negative regulator for inflammation, and the occurrence of ISR in Chinese patients. METHODS: We enrolled patients with coronary artery disease who underwent PCI with stenting. PCI procedures were performed successfully and a follow-up angiography was repeated 1 year later to determine ISR presence...
August 22, 2017: Oncotarget
Yu-Chan Ahn, Jeehoon Han
A novel strategy of large-scale 1,4-pentanediol (1,4-PDO) production derived from corn stover is presented based on catalytic conversion experiments. In this strategy, cellulose and hemicellulose of corn stover are catalytically converted to GVL by using corn stover derived GVL asa reaction solvent, and GVL asa reaction intermediate is then upgraded to 1,4-PDO. In the strategy, three possible designs consisting of conversion and separation subsystems are developed in terms of biomass residues as electricity or fuel sources (A: electricity source, B: electricity and fuel sources, and C: fuel source)...
September 5, 2017: Bioresource Technology
Anja Jäschke, Boubacar Coulibaly, Edmond J Remarque, Hermann Bujard, Christian Epp
Naturally acquired immunity against malaria is largely mediated by serum antibodies controlling levels of blood stage parasites. A limited understanding of the antigenic targets and functional mechanisms of protective antibodies has hampered so far the development of efficient malaria vaccines. Besides directly inhibiting growth of Plasmodium parasites, antibodies can opsonize merozoites and recruit immune effector cells such as monocytes or neutrophils. Antibodies against the vaccine candidate merozoite surface protein-1 (MSP-1) are acquired during natural infection and have been associated with protection against malaria in several epidemiological studies...
September 6, 2017: Clinical and Vaccine Immunology: CVI
Olga Otrusinová, Gabriel Demo, Petr Padrta, Zuzana Jaseňáková, Blanka Pekárová, Zuzana Gelová, Agnieszka Szmitkowska, Pavel Kadeřávek, Séverine Jansen, Milan Zachrdla, Tomáš Klumpler, Jaromír Marek, Jozef Hritz, Lubomír Janda, Hideo Iwaï, Michaela Wimmerová, Jan Hejátko, Lukáš Žídek
Multistep phosphorelay (MSP) cascades mediate responses to a wide spectrum of stimuli, including plant hormonal signaling, but several aspects of MSP await elucidation. Here, we provide first insight into the key step of MSP-mediated phosphotransfer in a eukaryotic system, the phosphorylation of the receiver domain of the histidine kinase CYTOKININ INDEPENDENT 1 (CKI1RD) from Arabidopsis thaliana We observed that the crystal structures of free, Mg(2+)-bound, and beryllofluoridated CKI1RD (a stable analog of the labile phosphorylated form) were identical and similar to the active state of receiver domains of bacterial response regulators...
August 31, 2017: Journal of Biological Chemistry
O A Olukosi, M M Kasprzak, S Kightley, P Carre, J Wiseman, J G M Houdijk
Four experiments were carried out to study the possible differences in metabolizable energy (ME) of meals (RSM) or expeller meals (RSE) from double-low rapeseed (Expt. 1), the influence of processing on ME (Expt. 2) and on relative phosphorus (P) bioavailability (Expt. 3) in RSM, and effect of RSM inclusion on growth performance of broilers (Expt. 4). For Expt. 1, diets with 300 g/kg RSM from 11 RSM and 4 RSE varieties were fed to broilers from d 14 to 21, with excreta collection on d 19 to 21. Each treatment had 8 replicates and 3 birds per replicate...
September 1, 2017: Poultry Science
Sehmus Ozden, Isaac G Macwan, Peter S Owuor, Suppanat Kosolwattana, Pedro A S Autreto, Sushila Silwal, Robert Vajtai, Chandra S Tiwary, Aditya D Mohite, Prabir K Patra, Pulickel M Ajayan
It is one of the most important needs to develop renewable, scalable and multifunctional methods for the fabrication of 3D carbon architectures. Even though a lot of methods have been developed to create porous and mechanically stable 3D scaffolds, the fabrication and control over the synthesis of such architectures still remain a challenge. Here, we used Magnetospirillum magneticum (AMB-1) bacteria as a bio-template to fabricate light-weight 3D solid structure of carbon nanotubes (CNTs) with interconnected porosity...
August 29, 2017: Scientific Reports
L Wang, X Z Liu, Z Y Ren, L Ding, J Nan, C L Liu, Z C Song, M J Feng, Q Yang, J T Wang
Objective: To explore the effect of polycyclic aromatic hydrocarbons (PAHs) and p16, FHIT gene CpG island methylation, as well as their interaction in cervical intraepithelial neoplasias. Methods: Objects of this study were from a cohort of cervical lesions study in Yangqu county of Shanxi province. All the patients were diagnosed pathologically, that including 83 patients with high-grade cervical intraepithelial neoplasia (CINⅡ/Ⅲ), 86 patients with low-grade cervical intraepithelial neoplasia (CINⅠ) and another 91 women under normal cervical (NC) condition...
August 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Zulkarnain Md Idris, Chim W Chan, James Kongere, Tom Hall, John Logedi, Jesse Gitaka, Chris Drakeley, Akira Kaneko
As markers of exposure anti-malaria antibody responses can help characterise heterogeneity in malaria transmission. In the present study antibody responses to Plasmodium falciparum AMA-1, MSP-119 and CSP were measured with the aim to describe transmission patterns in meso-endemic settings in Lake Victoria. Two cross-sectional surveys were conducted in Lake Victoria in January and August 2012. The study area comprised of three settings: mainland (Ungoye), large island (Mfangano) and small islands (Takawiri, Kibuogi, Ngodhe)...
August 22, 2017: Scientific Reports
Junlin Zhang, Lilan Xie, Dingfeng Xu, Shuohao Yue, Yi Li, Xiaohong Guo, Xiaojing Lai
Although purified and synthesized Cecropin A-magainin 2 (CAMA-syn) shows potent antibacterial activity in vitro, its ability to inhibit bacteria within mammal cells mediated by virus vector has not yet been investigated. To enhance its antimicrobial potential and reduce systemic side effects, it would be desirable to deliver CAMA-syn in macrophages by adenovirus vector. In this study,recombinant adenovirus Ad-MSP-CAMA/GFP were used to infect macrophages RAW264.7 cells in vitro and macrophages cells of lungs in vivo and the expression of CAMA-syn was detected by RT-PCR and observation of co-expression of GFP...
September 30, 2017: Gene
Yingjie Zhang, Huibo Li, Rongyi Cao, Lili Sun, Yan Wang, Shengjin Fan, Yanqiu Zhao, Desheng Kong, Lin Cui, Leilei Lin, Ke Wang, Yinghua Li, Jin Zhou
Inactivation of Dickkopf-3 (DKK3) is closely associated with a poor prognosis in various solid tumor and hematologic malignancies. Promoter hypermethylation is one potential cause of DKK3 inactivation. However, whether other mechanisms lead to DKK3 inactivation and the subsequent effects of these inactivations on cell proliferation and the Wnt signaling pathway in adult B acute lymphoblastic leukemia (B-ALL) remain unclear. In the present study, we found that low DKK3 expression levels were associated with high miR-708 expression and promoter hypermethylation in adult B-ALL...
July 18, 2017: Oncotarget
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