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https://www.readbyqxmd.com/read/28720100/effect-of-seasonal-malaria-chemoprevention-on-the-acquisition-of-antibodies-to-plasmodium-falciparum-antigens-in-ouelessebougou-mali
#1
Almahamoudou Mahamar, Djibrilla Issiaka, Amadou Barry, Oumar Attaher, Adama B Dembele, Tiangoua Traore, Adama Sissoko, Sekouba Keita, Bacary Soumana Diarra, David L Narum, Patrick E Duffy, Alassane Dicko, Michal Fried
BACKGROUND: Seasonal malaria chemoprevention (SMC) is a new strategy to reduce malaria burden in young children in Sahelian countries. It consists of the administration of full treatment courses of sulfadoxine-pyrimethamine plus amodiaquine to children at monthly intervals during the malaria season. However, it is not clear if there is a cumulative effect of SMC over time on acquisition of antibodies to malaria antigens. METHODS: A cross-sectional serosurvey was carried out 1 month after the last dose of SMC in 2016...
July 18, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28698274/noroviruses-co-opt-the-function-of-host-proteins-vapa-and-vapb-for-replication-via-a-phenylalanine-phenylalanine-acidic-tract-motif-mimic-in-nonstructural-viral-protein-ns1-2
#2
Broc T McCune, Wei Tang, Jia Lu, James B Eaglesham, Lucy Thorne, Anne E Mayer, Emily Condiff, Timothy J Nice, Ian Goodfellow, Andrzej M Krezel, Herbert W Virgin
The Norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. Murine noroviruses provide the opportunity to study norovirus replication in cell culture and in small animals. The human norovirus nonstructural protein NS1/2 interacts with the host protein VAMP-associated protein A (VAPA), but the significance of the NS1/2-VAPA interaction is unexplored. Here we report decreased murine norovirus replication in VAPA- and VAPB-deficient cells. We characterized the role of VAPA in detail...
July 11, 2017: MBio
https://www.readbyqxmd.com/read/28693156/detection-of-opcml-methylation-a-possible-epigenetic-marker-from-free-serum-circulating-dna-to-improve-the-diagnosis-of-early-stage-ovarian-epithelial-cancer
#3
Bi Wang, Lei Yu, Xin Luo, Lin Huang, Qin-Shan Li, Xiao-Shan Shao, Yi Liu, Yu Fan, Guo-Zhen Yang
The aim of the present study was to identify the appropriate DNA sequence and design high-quality primers for methylation-specific polymerase chain reaction (MSP). These primers may be used to examine and identify patients with early-stage epithelial ovarian carcinoma (EOC). Opioid binding protein/cell adhesion molecule like (OPCML), Runt-related transcription factor 3 and tissue factor pathway inhibitor 2 were selected as possible molecular markers. MSP primer sets were designed to monitor the methylation of the three markers...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28678324/screening-and-identification-of-a-tumor-specific-methylation-phenotype-in-the-colorectal-laterally-spreading-tumor
#4
H-B Ni, F-Y Wang, J Xu, X-J He, J Chen, Q Wu, J-F Wu, Y-S Sun
OBJECTIVE: We screened and identified the differential expression of the methylation phenotype in the whole genome of colorectal laterally spreading tumor (LSTs). MATERIALS AND METHODS: 3 tissue samples of colorectal polypoid adenomas (PAs), 3 tissue samples of LSTs and 3 tissue samples of colon cancer were analyzed with a high-density gene chip, and about 450,000 methylation sites were detected covering approximately 95% of the CpG islands. The Delta Data screening was taken through a cluster analysis of methylation phenotype differential expression...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28656627/cb1-and-ethanol-effects-on-glutamatergic-transmission-in-the-central-amygdala-of-male-and-female-msp-and-wistar-rats
#5
Dean Kirson, Christopher Shaun Oleata, Loren Howell Parsons, Roberto Ciccocioppo, Marisa Roberto
The central amygdala (CeA) is involved in the processing of anxiety and stress and plays a role in ethanol consumption. Chronic ethanol recruits stress systems in the CeA, leading to aversive withdrawal symptoms. Although primarily GABAergic, CeA contains glutamatergic afferents, and we have reported inhibitory effects of ethanol on locally evoked glutamatergic responses in CeA of Wistar and Marchigian Sardinian alcohol-preferring (msP) rats. Notably, msP rats display enhanced anxiety, stress and alcohol drinking, simulating the alcohol-dependent phenotype...
June 28, 2017: Addiction Biology
https://www.readbyqxmd.com/read/28634272/the-secreted-msp-domain-of-c-elegans-vapb-homolog-vpr-1-patterns-the-adult-striated-muscle-mitochondrial-reticulum-via-smn-1
#6
Jessica Schultz, Se-Jin Lee, Tim Cole, Hieu D Hoang, Jack Vibbert, Pauline A Cottee, Michael A Miller, Sung Min Han
The major sperm protein domain (MSPd) has an extracellular signaling function implicated in amyotrophic lateral sclerosis. Secreted MSPds derived from the C. elegans VAPB homolog VPR-1 promote mitochondrial localization to actin-rich I-bands in body wall muscle. Here we show that the nervous system and germ line are key MSPd secretion tissues. MSPd signals are transduced through the CLR-1 Lar-like tyrosine phosphatase receptor. We show that CLR-1 is expressed throughout the muscle plasma membrane, where it is accessible to MSPd within the pseudocoelomic fluid...
June 15, 2017: Development
https://www.readbyqxmd.com/read/28606809/animal-derived-natural-products-of-sowa-rigpa-medicine-their-pharmacopoeial-description-current-utilization-and-zoological-identification
#7
Karma Yeshi, Paolo Morisco, Phurpa Wangchuk
ETHNOPHARMACOLOGICAL RELEVANCE: The Bhutanese Sowa Rigpa medicine (BSM) uses animal parts in the preparation of numerous polyingredient traditional remedies. Our study reports the taxonomical identification of medicinal animals and the description of traditional uses in English medical terminologies. AIM OF THE STUDY: To taxonomically identify the medicinal animals and their derived natural products used as a zootherapeutic agents in BSM. MATERIALS AND METHODS: First, the traditional textbooks were reviewed to generate a list of animal products described as ingredients...
June 9, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28576864/lin-41-and-oma-ribonucleoprotein-complexes-mediate-a-translational-repression-to-activation-switch-controlling-oocyte-meiotic-maturation-and-the-oocyte-to-embryo-transition-in-caenorhabditis-elegans
#8
Tatsuya Tsukamoto, Micah D Gearhart, Caroline A Spike, Gabriela Huelgas-Morales, Makaela Mews, Peter R Boag, Traude H Beilharz, David Greenstein
An extended meiotic prophase is a hallmark of oogenesis. Hormonal signaling activates the CDK1/cyclin B kinase to promote oocyte meiotic maturation, which involves nuclear and cytoplasmic events. Nuclear maturation encompasses nuclear envelope breakdown, meiotic spindle assembly, and chromosome segregation. Cytoplasmic maturation involves major changes in oocyte protein translation and cytoplasmic organelles and is poorly understood. In the nematode Caenorhabditis elegans, sperm release the major sperm protein (MSP) hormone to promote oocyte growth and meiotic maturation...
June 1, 2017: Genetics
https://www.readbyqxmd.com/read/28554048/ascorbic-acid-mediated-enhanced-cardiomyocyte-differentiation-of-mouse-es-cells-involves-interplay-of-dna-methylation-and-multiple-signals
#9
Deepti Abbey, Polani B Seshagiri
Embryonic stem cells (ES-cells) provide a good model system to study lineage-specific differentiation. Though, the differentiation of ES-cells to cardiomyocytes is documented, a clear understanding of the molecular mechanism of differentiation and improved functional-differentiation efficiency are yet to be achieved. In this regard, ascorbic acid (Aa) is shown to be one of the effective cardiac inducers in ES-cells. But, its mechanism is poorly understood. We therefore, investigated the mechanism of Aa-mediated cardiomyocyte differentiation of ES-cells...
May 10, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/28549390/clinical-and-molecular-monitoring-of-plasmodium-falciparum-resistance-to-antimalarial-drug-artesunate-sulphadoxine-pyrimethamine-in-two-highly-malarious-district-of-madhya-pradesh-central-india-from-2012-2014
#10
Sweta Mishra, Praveen K Bharti, Man M Shukla, Nazia A Ali, Sher S Kashyotia, Avdhesh Kumar, Akshay C Dhariwal, Neeru Singh
The spread of P. falciparum resistant strain has led to a significant resurgence of malaria morbidity and mortality. The current cornerstone in malaria treatment in India is Artemisinin based Combination (Artesunate + Sulphadoxine-Pyrimethamine) Therapy (ACT) for treatment of uncomplicated P. falciparum malaria since 2010. In the present study we assessed the therapeutic efficacy of ACT and molecular monitoring of antimalarial resistance. Therapeutic efficacy was determined by in vivo method using 28 days follow-up...
June 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28542247/genetic-polymorphism-of-merozoite-surface-protein-2-msp-2-in-plasmodium-falciparum-isolates-from-pawe-district-north-west-ethiopia
#11
Hussein Mohammed, Moges Kassa, Ashenafi Assefa, Mekonnen Tadesse, Amha Kebede
BACKGROUND: In malaria endemic regions, Plasmodium falciparum infection is characterized by extensive genetic diversity. Describing this diversity provides important information about the local malaria situation. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Pawe district, North West Ethiopia, using the highly polymorphic merozoite surface protein 2 gene. METHODS: Atotal of 92 isolates from patients with uncomplicated P...
2017: PloS One
https://www.readbyqxmd.com/read/28500887/integrated-techno-economic-and-environmental-analysis-of-butadiene-production-from-biomass
#12
Somayeh Farzad, Mohsen Ali Mandegari, Johann F Görgens
In this study, lignocellulose biorefineries annexed to a typical sugar mill were investigated to produce either ethanol (EtOH) or 1,3-butadiene (BD), utilizing bagasse and trash as feedstock. Aspen simulation of the scenarios were developed and evaluated in terms of economic and environmental performance. The minimum selling prices (MSPs) for bio-based BD and EtOH production were 2.9-3.3 and 1.26-1.38-fold higher than market prices, respectively. Based on the sensitivity analysis results, capital investment, Internal Rate of Return and extension of annual operating time had the greatest impact on the MSP...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28482119/silencing-of-homeobox-a5-gene-in-the-stratum-corneum-of-psoriasis
#13
Yoshimasa Nobeyama, Yoshinori Umezawa, Hidemi Nakagawa
Analysis of psoriatic parakeratotic cells is helpful for understanding the pathogenesis of psoriasis. Methylation analysis can be performed on psoriatic scales, but it is unclear whether genes can be silenced by DNA methylation in psoriatic stratum corneum. The present study was conducted to detect genes silenced in psoriatic stratum corneum. Methylation array analysis with 485,577 probes, quantitative real-time methylation-specific PCR (RT-MSP) and bisulfite sequencing were performed for 30 psoriatic scale samples, 6 fully developed psoriatic skin samples and 12 normal skin samples...
May 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28469808/methylation-of-kruppel-like-factor-2-klf2-associates-with-its-expression-and-non-small-cell-lung-cancer-progression
#14
Wenbin Jiang, Xueqing Xu, Shaoli Deng, Jie Luo, Huan Xu, Chao Wang, Tingting Sun, Guoqin Lei, Fengling Zhang, Cheng Yang, Lin Zhou, Feng Wang, Ming Chen
Kruppel-like factor 2 (KLF2) is a putative tumor suppressor gene. This study investigated its role and epigenetic mechanisms in human non-small cell lung cancer (NSCLC) in ex vivo and in vitro. A total of 47 paired NSCLC and normal tissues and six cell lines were analyzed using qRT-PCR for KLF2 expression. KLF2 methylation was assessed using the methylation specific PCR (MSP) or bisulfite sequencing PCR (BSP). Functional KLF2 region 4 (+567 to +906) was confirmed using the dual-luciferase reporter assay, while CCK-8 cell viability and flow cytometric assays were used to assess changes in cell viability, cell cycle distribution, and apoptosis after knockdown or re-expression of KLF2...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28453906/oral-treponeme-major-surface-protein-sequence-diversity-and-distributions-within-periodontal-niches
#15
Meng You, Yuki Chan, Donnabella C Lacap-Bugler, Yong-Biao Huo, Wenling Gao, W Keung Leung, Rory M Watt
Treponema denticola and other species (phylotypes) of oral spirochetes are widely-considered to play important etiological roles in periodontitis and other oral infections. The major surface protein (Msp) of T. denticola is directly implicated in several pathological mechanisms. Here, we have analyzed msp sequence diversity across 68 strains of oral phylogroup 1 and 2 treponemes; including reference strains of T. denticola, Treponema putidum, Treponema medium, 'Treponema vincentii', and 'Treponema sinensis'...
April 28, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28440432/ron-and-c-met-facilitate-metastasis-through-the-erk-signaling-pathway-in-prostate-cancer-cells
#16
Binbin Yin, Zhenping Liu, Yiyun Wang, Xuchu Wang, Weiwei Liu, Pan Yu, Xiuzhi Duan, Chunhua Liu, Yuhua Chen, Yurong Zhang, Xiaoyan Pan, Hangping Yao, Zhaoping Liao, Zhihua Tao
Prostate cancer (PCa) is a metastatic malignant cancer driven by complex pathological mechanisms and characterized by poor long-term prognosis. Metastasis is the main cause of death of PCa patients, yet the molecular mechanisms of this process are poorly understood. In the present study, positive co-expression of RON and c-Met was observed in human clinical PCa tissues (biopsy material), as detected by immunohistochemical staining and quantitative real-time PCR. We investigated this further in PCa cells, demonstrating that the inhibition of RON and c-Met with foretinib (GSK1363089) suppressed metastasis and promoted the reversal of the epithelial-to-mesenchymal transition (EMT) in PCa cells...
June 2017: Oncology Reports
https://www.readbyqxmd.com/read/28365691/dickkopf-related-protein-2-is-epigenetically-inactivated-and-suppresses-colorectal-cancer-growth-and-tumor-metastasis-by-antagonizing-wnt-%C3%AE-catenin-signaling
#17
Can Wang, Yujuan Yue, Bianfei Shao, Zhu Qiu, Junhao Mu, Jun Tang, Xiaofan Han, Tingxiu Xiang, Guosheng Ren
BACKGROUND/AIMS: Aberrant activation of the Wnt/β-catenin signaling pathway plays a key role in the pathogenesis of multiple tumors including digestive cancers. Recent studies have reported that Dickkopf-related protein 2 (DKK2) is epigenetically inactivated in numerous types of cancers and that its gene products exhibit tumor-suppressive properties. However, the biological functions and underlying molecular mechanisms of DKK2 in colon carcinoma remains obscure. METHODS: We examined the expression of DKK2 in colon tumor cell lines by RT-PCR and its promoter methylation status in colon tumor cell lines and primary tumors by methylation-specific PCR (MSP)...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28350481/a-qualitative-study-of-a-maintenance-support-program-for-women-at-risk-of-homelessness-part-2-situational-factors
#18
Rose McMaster, Violeta Lopez, Rachel Kornhaber, Michelle Cleary
People who are homeless tend to have a number of complex needs. A housing maintenance support program (MSP) for women from the perspectives of clients, case managers and health professionals within the program was explored in this qualitative descriptive study. Interviews were conducted, and data were analysed using thematic analysis. The overarching theme that emerged from the data was "A life-changing event: I have the power to change." This theme was supported by three sub-themes: personal, situational and societal dimensions...
June 2017: Issues in Mental Health Nursing
https://www.readbyqxmd.com/read/28341016/the-role-of-alcohol-expectancies-in-sexual-risk-behaviors-among-adolescents-and-young-adults-in-the-democratic-republic%C3%A2-of-the%C3%A2-congo
#19
Emily A Hurley, Heena Brahmbhatt, Patrick K Kayembe, Michelle-Alphonsine Fatuma Busangu, Ma-Umba Mabiala, Deanna Kerrigan
PURPOSE: Considering the increased risk of HIV among alcohol users, we explored the role of individual alcohol expectancies in risk behaviors among youth in the Democratic Republic of the Congo. METHODS: We surveyed 1,396 adolescents (ages 15-19) and young adults (ages 20-24) on alcohol and sexual behaviors in May, 2010. We assessed expectancies of alcohol use (1) leading to sex or positive sexual experiences; (2) diminishing one's ability to resist unwanted sex; and (3) diminishing one's ability to use or negotiate use of condoms...
January 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/28336320/preliminary-research-on-dna-methylation-changes-during-murine-palatogenesis-induced-by-tcdd
#20
Chen Wang, Xin-Gang Yuan, Cui-Ping Liu, Sha-Na Zhai, Ding-Wen Zhang, Yue-Xian Fu
2,3,7,8-Tetrachlrodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate through growth factor and receptor expression changes during palatogenesis. DNA methylation is an important epigenetic modification that can regulate gene expressions and may be involved in TCDD-induced cleft palate. In this study, we investigated the effects of TCDD on the global and CpG DNA methylation status and the expression levels of DNA methyltransferases (Dnmts) in palate tissue of fetal mice. Pregnant C57BL/6J mice were administered with corn oil or TCDD 28 μg/kg at gestation day 10...
February 12, 2017: Journal of Cranio-maxillo-facial Surgery
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