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https://www.readbyqxmd.com/read/29035457/evaluation-of-mir-203-expression-levels-and-dna-promoter-methylation-status-in-serum-of-patients-with-endometrial-cancer
#1
Marco Benati, Martina Montagnana, Elisa Danese, Elisa Paviati, Silvia Giudici, Massimo Franchi, Giuseppe Lippi
BACKGROUND: Endometrial cancer (EC) is currently considered the fourth most frequent female cancer in Europe. In an attempt to achieve an early diagnosis, many studies have identified some putative biomarkers for gynecologic cancers, including circulating microRNAs (miRs) and aberrant promoter methylation status. Previous studies which have investigated miR-203 expression profiles in EC tissues and normal endometrial tissues concluded that miR-203 is regulated by methylation promoter...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29026785/evaluation-of-p16-ink4%C3%AE-hypermethylation-from-liquid-based-pap-test-samples-in-vietnamese-population
#2
Phuong Kim Truong, Thuan Duc Lao, Thuy Ai Huyen LE
BACKGROUND: Human papillomavirus (HPV) infection has been considered as main cause of cervical cancer. Recently, aberrant DNA methylation at tumor suppressor genes (TSGs), leading to inactivation, has also been an early epigenetic event and cofactor in cervical carcinogenesis. This study was performed to evaluate an association between the hypermethylation of p16(INK4α) gene's promoter and HPV exposure in non-invasive samples (liquid-based papanicolaous samples) in Vietnamese population...
September 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29026066/aberrant-promoter-methylation-of-protocadherin8-pcdh8-in-serum-is-a-potential-prognostic-marker-for-low-gleason-score-prostate-cancer
#3
Ying-Li Lin, Yan-Li Li, Jian-Guo Ma
BACKGROUND PCDH8 is a newly-discovered suppressor gene that is frequently inactivated by aberrant methylation in several human cancers, including prostate cancer. The identification of PCDH8 methylation can be used as a potential predictive biomarker. Prostate cancer patients with high Gleason score are considered as being at high risk for tumor recurrence and progression, and adjuvant therapy is often routinely performed in clinical practice. In the present study, we did not measure the methylation of PCDH8 in these patients...
October 13, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28988194/observational-study-of-the-populations-accessing-rapid-point-of-care-hiv-testing-in-winnipeg-manitoba-canada-through-a-retrospective-chart-review-of-site-records
#4
James Blain Johnston, Joss N Reimer, John L Wylie, Jared Bullard
OBJECTIVES: HIV point-of-care testing (POCT) has been available in Manitoba since 2008. This study evaluated the effectiveness of POCT at identifying individuals with previously unknown HIV status, its effects on clinical outcomes and the characteristics of the populations reached. METHODS: A retrospective database review was conducted for individuals who received HIV POCT from 2011 to 2014. Time to linkage to care and viral load suppression were compared between individuals who tested positive for HIV using POCT and controls identified as positive through standard screening...
October 7, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28948158/self-assembly-of-nanoscale-particles-with-biosurfactants-and-membrane-scaffold-proteins
#5
Ramona Faas, Annelie Pohle, Karin Moß, Marius Henkel, Rudolf Hausmann
Nanodiscs are membrane mimetics which may be used as tools for biochemical and biophysical studies of a variety of membrane proteins. These nanoscale structures are composed of a phospholipid bilayer held together by an amphipathic membrane scaffold protein (MSP). In the past, nanodiscs were successfully assembled with membrane scaffold protein 1D1 and 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine with a homogeneous diameter of ∼10 nm. In this study, the formation of nanoscale particles from MSP1D1 and rhamnolipid biosurfactants is investigated...
December 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28860196/conformational-dynamics-as-a-key-factor-of-signaling-mediated-by-the-receiver-domain-of-sensor-histidine-kinase-from-arabidopsis-thaliana
#6
Olga Otrusinová, Gabriel Demo, Petr Padrta, Zuzana Jaseňáková, Blanka Pekárová, Zuzana Gelová, Agnieszka Szmitkowska, Pavel Kadeřávek, Séverine Jansen, Milan Zachrdla, Tomáš Klumpler, Jaromír Marek, Jozef Hritz, Lubomír Janda, Hideo Iwaï, Michaela Wimmerová, Jan Hejátko, Lukáš Žídek
Multistep phosphorelay (MSP) cascades mediate responses to a wide spectrum of stimuli, including plant hormonal signaling, but several aspects of MSP await elucidation. Here, we provide first insight into the key step of MSP-mediated phosphotransfer in a eukaryotic system, the phosphorylation of the receiver domain of the histidine kinase CYTOKININ INDEPENDENT 1 (CKI1RD) from Arabidopsis thaliana We observed that the crystal structures of free, Mg(2+)-bound, and beryllofluoridated CKI1RD (a stable analog of the labile phosphorylated form) were identical and similar to the active state of receiver domains of bacterial response regulators...
August 31, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28854758/investigations-of-the-nutritive-value-of-meals-of-double-low-rapeseed-and-its-influence-on-growth-performance-of-broiler-chickens
#7
O A Olukosi, M M Kasprzak, S Kightley, P Carre, J Wiseman, J G M Houdijk
Four experiments were carried out to study the possible differences in metabolizable energy (ME) of meals (RSM) or expeller meals (RSE) from double-low rapeseed (Expt. 1), the influence of processing on ME (Expt. 2) and on relative phosphorus (P) bioavailability (Expt. 3) in RSM, and effect of RSM inclusion on growth performance of broilers (Expt. 4). For Expt. 1, diets with 300 g/kg RSM from 11 RSM and 4 RSE varieties were fed to broilers from d 14 to 21, with excreta collection on d 19 to 21. Each treatment had 8 replicates and 3 birds per replicate...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28847065/-interaction-between-polycyclic-aromatic-hydrocarbons-and-p16-fhit-gene-cpg-island-methylation-in-patients-with-cervical-intraepithelial-neoplasias
#8
L Wang, X Z Liu, Z Y Ren, L Ding, J Nan, C L Liu, Z C Song, M J Feng, Q Yang, J T Wang
Objective: To explore the effect of polycyclic aromatic hydrocarbons (PAHs) and p16, FHIT gene CpG island methylation, as well as their interaction in cervical intraepithelial neoplasias. Methods: Objects of this study were from a cohort of cervical lesions study in Yangqu county of Shanxi province. All the patients were diagnosed pathologically, that including 83 patients with high-grade cervical intraepithelial neoplasia (CINⅡ/Ⅲ), 86 patients with low-grade cervical intraepithelial neoplasia (CINⅠ) and another 91 women under normal cervical (NC) condition...
August 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28827116/targeting-expression-of-antimicrobial-peptide-cama-syn-by-adenovirus-vector-in-macrophages-inhibits-the-growth-of-intracellular-bacteria
#9
Junlin Zhang, Lilan Xie, Dingfeng Xu, Shuohao Yue, Yi Li, Xiaohong Guo, Xiaojing Lai
Although purified and synthesized Cecropin A-magainin 2 (CAMA-syn) shows potent antibacterial activity in vitro, its ability to inhibit bacteria within mammal cells mediated by virus vector has not yet been investigated. To enhance its antimicrobial potential and reduce systemic side effects, it would be desirable to deliver CAMA-syn in macrophages by adenovirus vector. In this study,recombinant adenovirus Ad-MSP-CAMA/GFP were used to infect macrophages RAW264.7 cells in vitro and macrophages cells of lungs in vivo and the expression of CAMA-syn was detected by RT-PCR and observation of co-expression of GFP...
September 30, 2017: Gene
https://www.readbyqxmd.com/read/28731690/the-metal-drives-the-chemistry-dual-functions-of-acireductone-dioxygenase
#10
REVIEW
Aditi R Deshpande, Thomas C Pochapsky, Dagmar Ringe
Acireductone dioxygenase (ARD) from the methionine salvage pathway (MSP) is a unique enzyme that exhibits dual chemistry determined solely by the identity of the divalent transition-metal ion (Fe(2+) or Ni(2+)) in the active site. The Fe(2+)-containing isozyme catalyzes the on-pathway reaction using substrates 1,2-dihydroxy-3-keto-5-methylthiopent-1-ene (acireductone) and dioxygen to generate formate and the ketoacid precursor of methionine, 2-keto-4-methylthiobutyrate, whereas the Ni(2+)-containing isozyme catalyzes an off-pathway shunt with the same substrates, generating methylthiopropionate, carbon monoxide, and formate...
August 9, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28720100/effect-of-seasonal-malaria-chemoprevention-on-the-acquisition-of-antibodies-to-plasmodium-falciparum-antigens-in-ouelessebougou-mali
#11
Almahamoudou Mahamar, Djibrilla Issiaka, Amadou Barry, Oumar Attaher, Adama B Dembele, Tiangoua Traore, Adama Sissoko, Sekouba Keita, Bacary Soumana Diarra, David L Narum, Patrick E Duffy, Alassane Dicko, Michal Fried
BACKGROUND: Seasonal malaria chemoprevention (SMC) is a new strategy to reduce malaria burden in young children in Sahelian countries. It consists of the administration of full treatment courses of sulfadoxine-pyrimethamine plus amodiaquine to children at monthly intervals during the malaria season. However, it is not clear if there is a cumulative effect of SMC over time on acquisition of antibodies to malaria antigens. METHODS: A cross-sectional serosurvey was carried out 1 month after the last dose of SMC in 2016...
July 18, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28698274/noroviruses-co-opt-the-function-of-host-proteins-vapa-and-vapb-for-replication-via-a-phenylalanine-phenylalanine-acidic-tract-motif-mimic-in-nonstructural-viral-protein-ns1-2
#12
Broc T McCune, Wei Tang, Jia Lu, James B Eaglesham, Lucy Thorne, Anne E Mayer, Emily Condiff, Timothy J Nice, Ian Goodfellow, Andrzej M Krezel, Herbert W Virgin
The Norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. Murine noroviruses provide the opportunity to study norovirus replication in cell culture and in small animals. The human norovirus nonstructural protein NS1/2 interacts with the host protein VAMP-associated protein A (VAPA), but the significance of the NS1/2-VAPA interaction is unexplored. Here we report decreased murine norovirus replication in VAPA- and VAPB-deficient cells. We characterized the role of VAPA in detail...
July 11, 2017: MBio
https://www.readbyqxmd.com/read/28693156/detection-of-opcml-methylation-a-possible-epigenetic-marker-from-free-serum-circulating-dna-to-improve-the-diagnosis-of-early-stage-ovarian-epithelial-cancer
#13
Bi Wang, Lei Yu, Xin Luo, Lin Huang, Qin-Shan Li, Xiao-Shan Shao, Yi Liu, Yu Fan, Guo-Zhen Yang
The aim of the present study was to identify the appropriate DNA sequence and design high-quality primers for methylation-specific polymerase chain reaction (MSP). These primers may be used to examine and identify patients with early-stage epithelial ovarian carcinoma (EOC). Opioid binding protein/cell adhesion molecule like (OPCML), Runt-related transcription factor 3 and tissue factor pathway inhibitor 2 were selected as possible molecular markers. MSP primer sets were designed to monitor the methylation of the three markers...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28678324/screening-and-identification-of-a-tumor-specific-methylation-phenotype-in-the-colorectal-laterally-spreading-tumor
#14
H-B Ni, F-Y Wang, J Xu, X-J He, J Chen, Q Wu, J-F Wu, Y-S Sun
OBJECTIVE: We screened and identified the differential expression of the methylation phenotype in the whole genome of colorectal laterally spreading tumor (LSTs). MATERIALS AND METHODS: 3 tissue samples of colorectal polypoid adenomas (PAs), 3 tissue samples of LSTs and 3 tissue samples of colon cancer were analyzed with a high-density gene chip, and about 450,000 methylation sites were detected covering approximately 95% of the CpG islands. The Delta Data screening was taken through a cluster analysis of methylation phenotype differential expression...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28656627/cb1-and-ethanol-effects-on-glutamatergic-transmission-in-the-central-amygdala-of-male-and-female-msp-and-wistar-rats
#15
Dean Kirson, Christopher Shaun Oleata, Loren Howell Parsons, Roberto Ciccocioppo, Marisa Roberto
The central amygdala (CeA) is involved in the processing of anxiety and stress and plays a role in ethanol consumption. Chronic ethanol recruits stress systems in the CeA, leading to aversive withdrawal symptoms. Although primarily GABAergic, CeA contains glutamatergic afferents, and we have reported inhibitory effects of ethanol on locally evoked glutamatergic responses in CeA of Wistar and Marchigian Sardinian alcohol-preferring (msP) rats. Notably, msP rats display enhanced anxiety, stress and alcohol drinking, simulating the alcohol-dependent phenotype...
June 28, 2017: Addiction Biology
https://www.readbyqxmd.com/read/28634272/the-secreted-msp-domain-of-c-elegans-vapb-homolog-vpr-1-patterns-the-adult-striated-muscle-mitochondrial-reticulum-via-smn-1
#16
Jessica Schultz, Se-Jin Lee, Tim Cole, Hieu D Hoang, Jack Vibbert, Pauline A Cottee, Michael A Miller, Sung Min Han
The major sperm protein domain (MSPd) has an extracellular signaling function implicated in amyotrophic lateral sclerosis. Secreted MSPds derived from the C. elegans VAPB homolog VPR-1 promote mitochondrial localization to actin-rich I-bands in body wall muscle. Here we show that the nervous system and germ line are key MSPd secretion tissues. MSPd signals are transduced through the CLR-1 Lar-like tyrosine phosphatase receptor. We show that CLR-1 is expressed throughout the muscle plasma membrane, where it is accessible to MSPd within the pseudocoelomic fluid...
June 15, 2017: Development
https://www.readbyqxmd.com/read/28606809/animal-derived-natural-products-of-sowa-rigpa-medicine-their-pharmacopoeial-description-current-utilization-and-zoological-identification
#17
Karma Yeshi, Paolo Morisco, Phurpa Wangchuk
ETHNOPHARMACOLOGICAL RELEVANCE: The Bhutanese Sowa Rigpa medicine (BSM) uses animal parts in the preparation of numerous polyingredient traditional remedies. Our study reports the taxonomical identification of medicinal animals and the description of traditional uses in English medical terminologies. AIM OF THE STUDY: To taxonomically identify the medicinal animals and their derived natural products used as a zootherapeutic agents in BSM. MATERIALS AND METHODS: First, the traditional textbooks were reviewed to generate a list of animal products described as ingredients...
June 9, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28576864/lin-41-and-oma-ribonucleoprotein-complexes-mediate-a-translational-repression-to-activation-switch-controlling-oocyte-meiotic-maturation-and-the-oocyte-to-embryo-transition-in-caenorhabditis-elegans
#18
Tatsuya Tsukamoto, Micah D Gearhart, Caroline A Spike, Gabriela Huelgas-Morales, Makaela Mews, Peter R Boag, Traude H Beilharz, David Greenstein
An extended meiotic prophase is a hallmark of oogenesis. Hormonal signaling activates the CDK1/cyclin B kinase to promote oocyte meiotic maturation, which involves nuclear and cytoplasmic events. Nuclear maturation encompasses nuclear envelope breakdown, meiotic spindle assembly, and chromosome segregation. Cytoplasmic maturation involves major changes in oocyte protein translation and cytoplasmic organelles and is poorly understood. In the nematode Caenorhabditis elegans, sperm release the major sperm protein (MSP) hormone to promote oocyte growth and meiotic maturation...
August 2017: Genetics
https://www.readbyqxmd.com/read/28554048/ascorbic-acid-mediated-enhanced-cardiomyocyte-differentiation-of-mouse-es-cells-involves-interplay-of-dna-methylation-and-multiple-signals
#19
Deepti Abbey, Polani B Seshagiri
Embryonic stem cells (ES-cells) provide a good model system to study lineage-specific differentiation. Though, the differentiation of ES-cells to cardiomyocytes is documented, a clear understanding of the molecular mechanism of differentiation and improved functional-differentiation efficiency are yet to be achieved. In this regard, ascorbic acid (Aa) is shown to be one of the effective cardiac inducers in ES-cells. But, its mechanism is poorly understood. We therefore, investigated the mechanism of Aa-mediated cardiomyocyte differentiation of ES-cells...
May 10, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/28549390/clinical-and-molecular-monitoring-of-plasmodium-falciparum-resistance-to-antimalarial-drug-artesunate-sulphadoxine-pyrimethamine-in-two-highly-malarious-district-of-madhya-pradesh-central-india-from-2012-2014
#20
Sweta Mishra, Praveen K Bharti, Man M Shukla, Nazia A Ali, Sher S Kashyotia, Avdhesh Kumar, Akshay C Dhariwal, Neeru Singh
The spread of P. falciparum resistant strain has led to a significant resurgence of malaria morbidity and mortality. The current cornerstone in malaria treatment in India is Artemisinin based Combination (Artesunate + Sulphadoxine-Pyrimethamine) Therapy (ACT) for treatment of uncomplicated P. falciparum malaria since 2010. In the present study we assessed the therapeutic efficacy of ACT and molecular monitoring of antimalarial resistance. Therapeutic efficacy was determined by in vivo method using 28 days follow-up...
June 2017: Pathogens and Global Health
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