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https://www.readbyqxmd.com/read/29455435/dna-methylation-regulated-micrornas-in-hpv-16-induced-head-and-neck-squamous-cell-carcinoma-hnscc
#1
M K Sannigrahi, Rajni Sharma, Varinder Singh, Naresh K Panda, Vidya Rattan, Madhu Khullar
INTRODUCTION: Epigenetic modifications have been reported to play an important role in regulating gene expression and these modifications become critical when they have a role in controlling another important layer of epigenetic regulation namely microRNAs. In the present study, we have identified the microRNAs that may be regulated by promoter DNA methylation and histone acetylation in Human papilloma virus-positive head and neck squamous cell carcinoma. METHODOLOGY: HPV-negative cell line (UPCI:SCC-116) and HPV-16 +ve cell line (UPCI:SCC-090) were treated with methylation inhibitor (5-aza-2'-deoxycytidine, AZA) and acetylation inhibitor (Trichostatin-A, TSA), followed by micro-array analysis...
February 17, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29449053/genetic-and-epigenetic-alterations-in-the-tumour-tumour-margins-and-normal-buccal-mucosa-of-patients-with-oral-cancer
#2
N Eljabo, N Nikolic, J Carkic, D Jelovac, M Lazarevic, N Tanic, J Milasin
Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers...
February 12, 2018: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29445424/promoter-methylation-of-dna-damage-repair-ddr-genes-in-human-tumor-entities-rbbp8-ctip-is-almost-exclusively-methylated-in-bladder-cancer
#3
Jolein Mijnes, Jürgen Veeck, Nadine T Gaisa, Eduard Burghardt, Tim C de Ruijter, Sonja Gostek, Edgar Dahl, David Pfister, Sebastian C Schmid, Ruth Knüchel, Michael Rose
Background: Genome-wide studies identified pan-cancer genes and shared biological networks affected by epigenetic dysregulation among diverse tumor entities. Here, we systematically screened for hypermethylation of DNA damage repair (DDR) genes in a comprehensive candidate-approach and exemplarily identify and validate candidate DDR genes as targets of epigenetic inactivation unique to bladder cancer (BLCA), which may serve as non-invasive biomarkers. Methods: Genome-wide DNA methylation datasets (2755 CpG probes of n = 7819 tumor and n = 659 normal samples) of the TCGA network covering 32 tumor entities were analyzed in silico for 177 DDR genes...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29434979/lats2-promoter-hypermethylation-and-its-effect-on-gene-expression-in-human-breast-cancer
#4
Saki Matsui, Naofumi Kagara, Chieko Mishima, Yasuto Naoi, Masafumi Shimoda, Atsushi Shimomura, Kenzo Shimazu, Seung Jin Kim, Shinzaburo Noguchi
Tumor-specific promoter hypermethylation of large tumor suppressor, homolog 2 (LATS2), a tumor suppressor gene, has been investigated using methylation-specific polymerase chain reaction (MSP) assays in different types of human cancer producing conflicting results. The aim of the present study was to evaluate the methylation status of the LATS2 promoter region using bisulfite sequencing with a next generation sequencer for breast cancer. In the 11 patients enrolled in the present study, the LATS2 promoter methylation index (MI) was uniformly high in tumor and normal tissues of the breast (median, 84...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29405146/identification-of-medically-important-candida-species-by-polymerase-chain-reaction-restriction-fragment-length-polymorphism-analysis-of-the-rdna-its1-and-its2-regions
#5
Suphi Bayraktar, Nizami Duran, Gülay Gülbol Duran, Naciye Eryilmaz, Hayat Aslan, Cansu Önlen, Burçin Özer
AIM: We aimed to identify the distribution of species in candidal strains isolated from clinical samples and restriction fragment length polymorphism (RFLP) method based on Msp I and Bln I restrictive enzyme cuts of polymerase chain reaction (PCR) products after the amplification of ITS1 and ITS2 regions of rDNA genotypically. MATERIALS AND METHODS: One hundred and fifty candidal strains isolated from various clinical samples were studies/ included. Phenotypic species assessment was performed using automated VITEK-2 system and kit used with the biochemical tests...
October 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29399169/histone-deacetylation-as-opposed-to-promoter-methylation-results-in-epigenetic-bim-silencing-and-resistance-to-egfr-tki-in-nsclc
#6
Mingchuan Zhao, Yishi Zhang, Jiayu Li, Xuefei Li, Ningning Cheng, Qi Wang, Weijing Cai, Chao Zhao, Yayi He, Jianhua Chang, Caicun Zhou
Drug resistance remains a major challenge in epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) therapy. Bcl-2-like protein 11 (BIM), a B-cell lymphoma 2 family pro-apoptotic protein, is a prime target for specific anti-cancer therapeutics. However, the epigenetic regulation of BIM in non-small cell lung cancer (NSCLC) cell lines and patients with NSCLC in association with EGFR-TKI resistance requires investigation. Methylation-specific PCR (MSP), pyrosequencing, and nested quantitative (q)-MSP were conducted to explore the methylation status of BIM in NSCLC cell lines...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29375686/correlation-between-rage-gene-promoter-methylation-and-diabetic-retinal-inflammation
#7
Shifeng Kan, Jing Wu, Chengxi Sun, Jing Hao, Zhen Wu
The methylation status of the receptor for advanced glycation end products (RAGE) gene promoter in peripheral blood mononuclear cells (PBMCs) of type 2 diabetic retinopathy (DR) patients was evaluated to investigate the correlation between RAGE gene promoter methylation and diabetic retinal inflammation. Eighty patients admitted and diagnosed as type 2 DR in Qilu Hospital, Shandong University during the period from October, 2013 to October, 2015 were enrolled in this study. They were the observation group and 40 healthy subjects were enrolled in the control group...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29355145/molecular-characterization-of-plasmodium-falciparum-in-arunachal-pradesh-from-northeast-india-based-on-merozoite-surface-protein-1-glutamate-rich-protein
#8
Nilanju Pran Sarmah, Kishore Sarma, Dibya Ranjan Bhattacharyya, Ali Sultan, Devendra Bansal, Neeru Singh, Praveen K Bharti, Hargobinder Kaur, Rakesh Sehgal, Pradyumna Kishore Mohapatra, Jagadish Mahanta
BACKGROUND & OBJECTIVES: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P...
September 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29344347/epigenetic-alterations-in-tramp-mice-epigenome-dna-methylation-profiling-using-medip-seq
#9
Wenji Li, Ying Huang, Davit Sargsyan, Tin Oo Khor, Yue Guo, Limin Shu, Anne Yuqing Yang, Chengyue Zhang, Ximena Paredes-Gonzalez, Michael Verzi, Ronald P Hart, Ah-Ng Kong
Purpose: We investigated the genomic DNA methylation profile of prostate cancer in transgenic adenocarcinoma of the mouse prostate (TRAMP) cancer model and to analyze the crosstalk among targeted genes and the related functional pathways. Methods: Prostate DNA samples from 24-week-old TRAMP and C57BL/6 male mice were isolated. The DNA methylation profiles were analyzed by methylated DNA immunoprecipitation (MeDIP) followed by next-generation sequencing (MeDIP-seq)...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29340524/efficacy-of-removal-of-cariogenic-bacteria-and-carious-dentin-by-ablation-using-different-modes-of-er-yag-lasers
#10
A Baraba, L Kqiku, D Gabrić, Ž Verzak, K Hanscho, I Miletić
The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Seventy-two extracted human molars were used in this study. Sixty teeth with carious dentin were randomly divided into four experimental groups according to the treatment for caries removal: group 1: 400 µs (FFC group); group 2: super short pulse (SSP group, 50 µs pulse); group 3: medium short pulse (MSP group, 100 µs pulse); group 4: short pulse (SP group, 300 µs pulse) and one positive control group with no treatment...
January 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29301521/training-the-next-generation-of-physician-researchers-vanderbilt-medical-scholars-program
#11
Abigail M Brown, Teresa M Chipps, Tebeb Gebretsadik, Lorraine B Ware, Jessica Y Islam, Luke R Finck, Joey Barnett, Tina V Hartert
BACKGROUND: As highlighted in recent reports published by the Physician-Scientist Workforce Working Group at the National Institutes of Health, the percentage of physicians conducting research has declined over the past decade. Various programs have been put in place to support and develop current medical student interest in research to alleviate this shortage, including The Vanderbilt University School of Medicine Medical Scholars Program (MSP). This report outlines the long-term program goals and short-term outcomes on career development of MSP alumni, to shed light on the effectiveness of research training programs during undergraduate medical training to inform similar programs in the United States...
January 4, 2018: BMC Medical Education
https://www.readbyqxmd.com/read/29301308/msp-4-an-antimicrobial-peptide-induces-apoptosis-via-activation-of-extrinsic-fas-fasl-and-intrinsic-mitochondria-mediated-pathways-in-one-osteosarcoma-cell-line
#12
Hsiao-Mei Kuo, Chung-Chih Tseng, Nan-Fu Chen, Ming-Hong Tai, Han-Chun Hung, Chien-Wei Feng, Shu-Yu Cheng, Shi-Ying Huang, Yen-Hsuan Jean, Zhi-Hong Wen
Osteosarcoma (OS) is a common malignant bone cancer. The relatively high density of a person's bone structure means low permeability for drugs, and so finding drugs that can be more effective is important and should not be delayed. MSPs are marine antimicrobial peptides (AMP) and natural compounds extracted from Nile tilapia (Oreochromis niloticus). MSP-4 is a part of the AMPs series, with the advantage of having a molecular weight of about 2.7-kDa and anticancer effects, although the responsible anticancer mechanism is not very clear...
January 2, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29286222/brca1-promoter-methylation-and-expression-associations-with-er-pr-and-her2-subtypes-of-breast-carcinoma
#13
Mohit Kumar, Ram Krishna Sahu, Aditi Goyal, Sonal Sharma, Navneet Kaur, Ravi Mehrotra, Usha Rani Singh, Suresh Hedau
Introduction: Considering the increasing trend in incidence rates, morbidity and mortality of breast cancer, there is an urgent need to identify and validate new biomarkers for early detection and better management. The purpose of the study was to investigate BRCA1 protein expression and promoter methylation of the BRCA1 gene and their association with molecular subtypes based on estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) positivity. Materials and Methods: A total of 114 breast cancer tissue biopsies were collected for methylation specific PCR (MSP) and immunohistochemical (IHC) analysis...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29285776/association-of-the-cyp17-msp-ai-t-34c-and-cyp19-codon-39-trp-arg-polymorphisms-with-susceptibility-to-acne-vulgaris
#14
F Chamaie-Nejad, S Saeidi, F Najafi, A Ebrahimi, Z Rahimi, E Shakiba, Z Rahimi
The aim of this study was to detect the association of the cytochrome P450 (CYP) 17 T-34C and CYP19 T<C polymorphisms with the risk of acne vulgaris (AV). The study enrolled 198 patients with AV (mild, moderate and severe) and 195 unrelated age-matched healthy controls from western Iran who had Kurdish ethnic background. The presence of the CYP17 TC genotype significantly increased the risk of mild, moderate and severe AV by 2.68, 2.28 and 2.94 times, respectively, while the presence of the CYP19 TC genotype significantly elevated the risk of overall AV and mild AV by 2...
December 28, 2017: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/29279840/pro-inflammatory-cytokine-response-and-genetic-diversity-in-merozoite-surface-protein-2-of-plasmodium-falciparum-isolates-from-nigeria
#15
Olusola Ajibaye, Akinniyi A Osuntoki, Albert Ot Ebuehi, Bamidele A Iwalokun, Emmanuel O Balogun, Kathleen N Egbuna
Background: Polymorphisms in Plasmodium falciparum merozoite surface protein-2 (msp-2) and associated parasite genetic diversity which varies between malaria-endemic regions remain a limitation in malaria vaccine development. Pro-inflammatory cytokines are important in immunity against malaria, understanding the influence of genetic diversity on cytokine response is important for effective vaccine design. Methods: P. falciparum isolates obtained from 300 Nigerians with uncomplicated falciparum malaria at Ijede General Hospital, Ijede (IJE), General Hospital Ajeromi, Ajeromi (AJE) and Saint Kizito Mission Hospital, Lekki, were genotyped by nested polymerase chain reaction of msp-2 block 3 while ELISA was used to determine the pro-inflammatory cytokine response to describe the genetic diversity of P...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29275584/-dna-methylation-modification-of-brms1-in-triple-negative-breast-cancer-and-its-correlation-with-tumor-metastasis
#16
J Xia, Z D Lü, P H Zhou, S C Cui, B Kong, D Liu, S S Long, X H Zhang, J J Zhao
Objective: To investigate the effect of methylation status of breast cancer metastasis suppressor gene 1 (BRMS1) on the expression of breast cancer and the biological behavior of cancer cells in triple-negative breast cancer (TNBC). Methods: The expression of BRMS1 in TNBC tissues and corresponding non-malignant tissues and its relationship with clinicopathological parameters were detected by immunohistochemistry. The mRNA and protein expression of BRMS1 in normal breast epithelial cells and TNBC cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting...
November 28, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29245188/the-unmet-needs-of-aboriginal-australians-with-musculoskeletal-pain-a-mixed-method-systematic-review
#17
Ivan B Lin, Samantha Bunzli, Donna B Mak, Charmaine Green, Roger Goucke, Juli Coffin, Peter B O'Sullivan
OBJECTIVE: Musculoskeletal pain (MSP) conditions are the biggest cause of disability and internationally, Indigenous peoples experience a higher burden. There are conflicting reports about Aboriginal Australians and MSP. We conducted a systematic review to describe the prevalence, associated factors, impacts, care access, health care experiences, and factors associated with MSP among Aboriginal Australians. METHODS: A systematic search of quantitative and qualitative scientific and grey literature (PROSPERO number: CRD42016038342)...
December 15, 2017: Arthritis Care & Research
https://www.readbyqxmd.com/read/29234405/qingchang-wenzhong-decoction-attenuates-dss-induced-colitis-in-rats-by-reducing-inflammation-and-improving-intestinal-barrier-function-via-upregulating-the-msp-ron-signalling-pathway
#18
Tangyou Mao, Junxiang Li, Lijuan Liu, Weihan Zhao, Yuyue Liu, Kangli Gao, Yi Guo, Tianhong Xie, Ningfei Li, Rui Shi
Ulcerative colitis (UC) is a chronic, nonspecific, inflammatory disease for which an effective treatment is lacking. Our previous study found that Qingchang Wenzhong Decoction (QCWZD) can significantly improve the clinical symptoms of UC and ameliorate dextran sulphate sodium- (DSS-) induced ulcerative colitis in rats by downregulating the IP10/CXCR3 axis-mediated inflammatory response. The purpose of the present study was to further explore the mechanism of QCWZD for UC in rats models, which were established by 7-day administration of 4...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29228451/rg108-induces-the-apoptosis-of-endometrial-cancer-ishikawa-cell-lines-by-inhibiting-the-expression-of-dnmt3b-and-demethylation-of-hmlh1
#19
L Yang, J Hou, X-H Cui, L-N Suo, Y-W Lv
OBJECTIVE: The effects of DNA methyltransferase (DNMT) inhibitor RG108 on the proliferation and apoptosis of endometrial cancer was investigated, and whether its mechanism was related to the inhibition of DNMT3B, thereby affecting the human mutL homolog 1 (hMLH1) methylation status and its expression, was further studied. MATERIALS AND METHODS: Culture of human endometrial cancer Ishikawa cell lines: cells grew adhering to the wall in Roswell Park Memorial Institute-1640 (RPMI-1640) medium (supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamic acid)...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29220586/co-occurring-psychosocial-problems-and-multiple-sexual-partners-among-men-who-have-sex-with-men-in-shanghai-china-a-syndemic-approach
#20
Zezhou Wang, Xu Zhao, Zhan Zhang, Mengyun Luo, Qiuming Shen, Yuanyuan Dong, Ying Wang, Yong Cai
We aimed to apply syndemic theory to verify the association and reinforcing effects between psychosocial problems and multiple sexual partners (MSP) of men who have sex with men (MSM) in Shanghai, China. A cross-sectional study was conducted among 547 MSM in Shanghai, China using a face-to-face survey. Sociodemographic and psychosocial data were collected, as well as information on MSP and condomless anal sex. In the 6 months prior to the survey, 54.3% of the MSM sample had had MSP. Univariate analysis showed that self-esteem, depression, loneliness, sexual compulsivity, and involuntary subordination (IS) were associated with MSP...
December 8, 2017: Journal of Sex Research
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