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https://www.readbyqxmd.com/read/29207829/evaluation-of-tumour-associated-macrophages-and-angiogenesis-in-ameloblastoma
#1
Akinyele O Adisa, Samuel E Udeabor, Anna Orlowska, Robert A Sader, Shahram Ghanaati
Introduction: Ameloblastoma is a locally invasive odontogenic neoplasm that has a high recurrence rate. The invasion of adjacent tissue is supported by angiogenesis stimulated by Tumour-Associated Macrophages (TAMs). TAMs are macrophages modified in the milieu of the tumour microenvironment and have very weak or no ability to present antigens. Thus, there is collaboration between the tumour and the tumour microenvironment to maintain tumour enlargement. TAMs exist as the classically activated M1 macrophages that possess antitumour activity and the otherwise activated M2 macrophages that support tumour invasion and metastasis...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29205549/identification-and-characterization-of-antibodies-elicited-by-human-cystatin-c-fragment
#2
Izabela Behrendt, Martyna Prądzińska, Marta Spodzieja, Paulina Czaplewska, Aleksandra S Kołodziejczyk, Aneta Szymańska, Franciszek Kasprzykowski, Susanna L Lundström, Roman A Zubarev, Sylwia Rodziewicz-Motowidło
Amyloid formation is associated with a number of neurodegenerative diseases that affect the independence and quality of life of aging populations. One of rather atypical, occurring at a young age amyloidosis is hereditary cystatin C amyloid angiopathy (HCCAA) related to aggregation of L68Q variant of human cystatin C (hCC). Human cystatin C plays a very important role in many aspects of human health; however, its amyloidogenic properties manifested in HCCAA present a real, lethal threat to some populations and any work on factors that can affect possible influencing hCC aggregation is not to overestimate...
December 5, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29199679/a-comparative-analysis-of-immunohistochemistry-and-fluorescent-in-situ-hybridization-assay-to-detect-anaplastic-lymphoma-kinase-status-in-lung-adenocarcinoma-cases-a-search-for-a-testing-algorithm
#3
P B Wagle, N A Jambhekar, R Kumar, K Prabhash, C S Pramesh, S B Desai, V Noronha, G Karimundackal, A Shah, A Joshi, S G Laskar, S Jiwnani, T Pai, J P Agarwal
INTRODUCTION: Testing for echinoderm microtubule-associated protein-like 4 (EML4) anaplastic lymphoma kinase (ALK) translocation by fluorescence in situ hybridization (FISH) is well established whereas the Food and Drug Administration (FDA) ALK immunohistochemical (IHC) test is relatively new. AIMS AND OBJECTIVE: The aim of this study is to compare FDA-approved ALK IHC test (D5F3 clone) with the standard ALK FISH test. MATERIALS AND METHODS: A validation and a test arm with 100 and 200 cases of Formalin-Fixed, Paraffin-embedded blocks of lung adenocarcinoma, respectively, comprised the material...
January 2017: Indian Journal of Cancer
https://www.readbyqxmd.com/read/29187883/programmed-cell-death-ligand-1-protein-levels-predicted-survival-of-non-small-cell-lung-cancer
#4
Shaohua Cui, Xinying Su, Lili Dong, Jialin Qian, Lin Ye, Tianwei Zhang, Haihua Fu, Hulin Han, Jiaqi Huang, Yihong Yao, Yi Gu, Liyan Jiang
Objective: To investigate the relationship between programmed death ligand 1 (PD-L1) expression using 5%, 25%, 50% cutoffs in tumor cells (TC) and postsurgical survival in non-small-cell lung cancer (NSCLC) patients. For samples with tumor infiltrating lymphocytes (TIL), correlation between PD-L1 expression in TIL using 1% cutoff and postsurgical survival was also evaluated. Methods: Primary NSCLC tumor surgical samples staging I to IIIA of 126 patients who underwent surgical procedures from September 2009 to August 2012 in Shanghai Chest Hospital, Shanghai Jiao Tong University were retrospectively included...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29187698/evaluation-of-schistosoma-japonicum-thioredoxin-peroxidase-1-as-a-potential-circulating-antigen-target-for-the-diagnosis-of-asian-schistosomiasis
#5
Adrian Miki C Macalanda, Jose Ma M Angeles, Kharleezelle J Moendeg, Anh Tm Dang, Luna Higuchi, Noboru Inoue, Xuenan Xuan, Masashi Kirinoki, Yuichi Chigusa, Lydia R Leonardo, Elena A Villacorte, Pilarita T Rivera, Yasuyuki Goto, Shin-Ichiro Kawazu
Asian schistosomiasis caused by Schistosoma japonicum is a serious zoonotic disease endemic in China, the Philippines and parts of Indonesia. Mass drug administration in endemic areas resulted to decline in disease severity and intensity. The low intensity of infection limits the use of current parasitological methods for schistosomiasis diagnosis. Detection of parasite circulating antigens might provide more informative result as it may indicate the true status of infection. In this study, S. japonicum thioredoxin peroxidase-1 (SjTPx-1) a 22 kDa secreted antioxidant enzyme expressed throughout the life stages of the parasite was evaluated for its potential use as a biomarker for schistosomiasis japonica infection...
November 28, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29162919/production-of-recombinant-human-procollagen-type-i-c-terminal-propeptide-and-establishment-of-a-sandwich-elisa-for-quantification
#6
Woo-Young Seo, Jeong-Ho Kim, Du-San Baek, Su-Jung Kim, Sujin Kang, Won Suk Yang, Ji-Ae Song, Moo-Seung Lee, Sunghoon Kim, Yong-Sung Kim
Procollagen type I carboxy-terminal propeptide (PICP), derived from type I procollagen, has been identified as an indicator of type I collagen synthesis in bone matrix formation and skin recovery. PICP is a heterotrimeric glycoprotein consisting of two α1 chains (PICPα1) and one α2 chain (PICPα2). Here, we report the recombinant expression of human PICP using a mammalian expression system. Co-expression of PICPα1 and PICPα2 in HEK293F cells resulted in the production of functional PICP in the correctly assembled heterotrimeric form...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29151654/development-and-evaluation-of-simple-dot-blot-assays-for-rapid-detection-of-staphylococcal-enterotoxin-a-in-food
#7
Mamta Singh, Ravi Kant Agrawal, Bhoj Raj Singh, Sanjod Kumar Mendiratta, Rajesh Kumar Agarwal, Mithilesh Kumar Singh, Deepak Kumar
The present study was aimed to develop and evaluate dot-blot assays for rapid detection of staphylococcal enterotoxin-A (SEA) in food. Dot blots were developed in two formats, indirect and sandwich utilizing mouse monoclonal anti-SEA and rabbit polyclonal anti-SEA antibodies. In indirect dot-blot format, recombinant SEA was directly coated on NCM dot-blot strip and detection was carried out by anti-SEA antibodies. In sandwich dot-blot format, SEA was trapped between anti-SEA capture and detection antibodies...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29135332/antibodies-to-pcpa-and-phtd-protect-mice-against-streptococcus-pneumoniae-by-a-macrophage-and-complement-dependent-mechanism
#8
Lucian Visan, Nicolas Rouleau, Emilie Proust, Loïc Peyrot, Arnaud Donadieu, Martina Ochs
Currently marketed Streptococcus pneumoniae (Spn) vaccines, which contain polysaccharide capsular antigens from the most common Spn serotypes, have substantially reduced pneumococcal disease rates but have limited coverage. A trivalent pneumococcal protein vaccine containing pneumococcal choline-binding protein A (PcpA), pneumococcal histidine triad protein D (PhtD), and detoxified pneumolysin is being developed to provide broader, cross-serotype protection. Antibodies against detoxified pneumolysin protect against bacterial pneumonia by neutralizing Spn-produced pneumolysin, but how anti-PhtD and anti-PcpA antibodies protect against Spn has not been established...
November 14, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29133571/development-of-protective-immunity-in-new-zealand-white-rabbits-challenged-with-bacillus-anthracis-spores-and-treated-with-antibiotics-and-obiltoxaximab-a-monoclonal-antibody-against-protective-antigen
#9
Lisa N Henning, Sarah Carpenter, Gregory V Stark, Natalya V Serbina
Recommended management of inhalational anthrax, a high-priority bioterrorist threat, includes antibiotics and antitoxins. Obiltoxaximab, a chimeric monoclonal antibody against anthrax Protective Antigen (PA) is licensed under the U.S. Food and Drug Administration's (FDA's) Animal Rule for treatment of inhalational anthrax. Because of spore latency, disease re-emergence after treatment cessation is a concern and there is a need to understand development of endogenous protective immune responses following antitoxin-containing anthrax treatment regimens...
November 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29127256/development-of-phospho-specific-rab-protein-antibodies-to-monitor-in-vivo-activity-of-the-lrrk2-parkinson-s-disease-kinase
#10
Pawel Lis, Sophie Burel, Martin Steger, Matthias Mann, Fiona Brown, Federico Diez, Francesca Tonelli, Janice L Holton, Philip Winglok Ho, Shu-Leong Ho, Meng-Yun Chou, Nicole K Polinski, Terina N Martinez, Paul Davies, Dario R Alessi
Mutations that activate the LRRK2 protein kinase, predispose to Parkinson's disease, suggesting that LRRK2 inhibitors might have therapeutic benefit. Recent work has revealed that LRRK2 phosphorylates a subgroup of 14 Rab proteins, including Rab10, at a specific residue located at the centre of its effector binding Switch-II motif. In this study, we analyse the selectivity and sensitivity of polyclonal and monoclonal phospho-specific antibodies raised against 9 different LRRK2 phosphorylated Rab proteins (Rab3A/3B/3C/3D, Rab5A/5B/5C, Rab8A/8B, Rab10, Rab12, Rab29[T71], Rab29[S72], Rab35 and Rab43)...
November 10, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29116503/construction-of-rabbit-immune-antibody-libraries
#11
Thi Thu Ha Nguyen, Jong Seo Lee, Hyunbo Shim
Rabbits have distinct advantages over mice as a source of target-specific antibodies. They produce higher affinity antibodies than mice, and may elicit strong immune response against antigens or epitopes that are poorly immunogenic or tolerated in mice. However, a great majority of currently available monoclonal antibodies are of murine origin because of the wider availability of murine fusion partner cell lines and well-established tools and protocols for fusion and cloning of mouse hybridoma. Phage-display selection of antibody libraries is an alternative method to hybridoma technology for the generation of target-specific monoclonal antibodies...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29115701/multispecificity-of-a-recombinant-anti-ras-monoclonal-antibody
#12
John W Schrader, Gary R McLean
Recombinant monoclonal antibodies (Ab's) have widespread application as research tools, diagnostic reagents and as biotherapeutics. Whilst studying the cellular molecular switch protein m-ras, a recombinant monoclonal antibody to m-ras was generated for use as a research tool. Antibody genes from a single rabbit B cell secreting IgG to an m-ras specific peptide sequence were expressed in mammalian cells, and monoclonal rabbit IgG binding was characterized by ELISA and peptide array blotting. Although the monoclonal Ab was selected for specificity to m-ras peptide, it also bound to both recombinant full-length m-ras and h-ras proteins...
November 8, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29115505/alanine-scanning-mutagenesis-of-sp70-epitope-in-characterizing-species%C3%A2-specific-antibodies-induced-by-enterovirus-71%C3%A2-based-antigens
#13
Meng Gao, Feng Zhang, Yun Zhu, Limei Gao, Yunshui Jiang, Yongneng Luo, Fangchang Zhuang, Zian Mao, Jiangsen Mao
The enterovirus 71 (EV71) SP70 epitope, derived from amino acids 208‑222 of VP1, is a neutralizing epitope. The present study aimed to assess the inter‑species differences of the antibodies induced by EV71‑based antigens in responses to SP70 mutant peptides. BALB/c mice and Lou/C rats were immunized with EV71 SP70. Monoclonal antibodies (Mabs) were produced by hybridoma clones. Serum polyclonal antibodies (Pabs) were produced from BALB/c mice and New Zealand white rabbits immunized with recombinant EV71 VP1 (rEV71‑VP1) protein or inactivated EV71...
November 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29090969/expression-of-cat-podoplanin-in-feline-squamous-cell-carcinomas
#14
Shunsuke Itai, Shinji Yamada, Mika K Kaneko, Hiroyuki Harada, Yumiko Kagawa, Satoru Konnai, Yukinari Kato
Oral squamous cell carcinoma is an aggressive tumor in cats; however, molecular-targeted therapies against this tumor, including antibody therapy, have not been developed. Sensitive and specific monoclonal antibodies (mAbs) against highly expressed membrane proteins are needed to develop antibody therapies. Podoplanin, a type I transmembrane glycoprotein, is expressed in many human malignant tumors, including brain tumor, esophageal cancer, lung cancer, mesothelioma, and oral cancer. Podoplanin binds to C-type lectin-like receptor-2 (CLEC-2) and activates platelet aggregation, which is involved in cancer metastasis...
November 1, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29089143/an-optimized-double-antibody-sandwich-elisa-for-quantitative-detection-of-wssv-in-artificially-infected-crayfish
#15
Xiaoqian Tang, Qianrong Liang, Lushan Liu, Xiuzhen Sheng, Jing Xing, Wenbin Zhan
Developing a rapid, accurate and quantitative method for detecting white spot syndrome virus (WSSV) is extremely urgent and critical for reducing the risk of white spot disease outbreaks. In the present work, an optimized double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed for quantitative detection of WSSV. The method employed rabbit polyclonal antibodies against WSSV as the capture antibody and previously produced anti-WSSV monoclonal antibodies as the detector antibody. A standard curve of the log concentration of WSSV versus OD value was established, which was linear in the concentration range of 120-7680ng/mL, and the linear regression equation was y=0...
January 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29072637/-morphology-of-epithelial-mesenchymal-transformation-in-neoplasias-of-oral-mucosa
#16
A A Ivina, V A Syomkin, I I Babichenko
The aim of the study was to assess correlation of E-cadherin and N-cadherin expression with proliferative activity and neoplasia grade in oral mucosa. Oral biopsies of 48 patients were studied with focal hyperplasia, squamous intraepithelial neoplasia and oral squamous cell carcinoma. Tissue antigens were determined using a mouse monoclonal antibody to Ki-67 and E-cadherin and rabbit polyclonal antibodies to N-cadherin. The study revealed correlation between the proliferative activity of epithelial cells and N-cadherin synthesis associated with decline of E-cadherin expression...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29035625/chickens-with-humanized-immunoglobulin-genes-generate-antibodies-with-high-affinity-and-broad-epitope-coverage-to-conserved-targets
#17
Kathryn H Ching, Ellen J Collarini, Yasmina N Abdiche, Daniel Bedinger, Darlene Pedersen, Shelley Izquierdo, Rian Harriman, Lei Zhu, Robert J Etches, Marie-Cecile van de Lavoir, William D Harriman, Philip A Leighton
Transgenic animal platforms for the discovery of human monoclonal antibodies have been developed in mice, rats, rabbits and cows. The immune response to human proteins is limited in these animals by their tolerance to mammalian-conserved epitopes. To expand the range of epitopes that are accessible, we have chosen an animal host that is less phylogenetically related to humans. Specifically, we generated transgenic chickens expressing antibodies from immunoglobulin heavy and light chain loci containing human variable regions and chicken constant regions...
October 16, 2017: MAbs
https://www.readbyqxmd.com/read/28993122/characterization-of-novel-monoclonal-antibodies-against-the-mers-coronavirus-spike-protein-and-their-application-in-species-independent-antibody-detection-by-competitive-elisa
#18
Shuetsu Fukushi, Aiko Fukuma, Takeshi Kurosu, Shumpei Watanabe, Masayuki Shimojima, Kazuya Shirato, Naoko Iwata-Yoshikawa, Noriyo Nagata, Kazuo Ohnishi, Manabu Ato, Simenew Keskes Melaku, Hiroshi Sentsui, Masayuki Saijo
Since discovering the Middle East respiratory syndrome coronavirus (MERS-CoV) as a causative agent of severe respiratory illness in the Middle East in 2012, serological testing has been conducted to assess antibody responses in patients and to investigate the zoonotic reservoir of the virus. Although the virus neutralization test is the gold standard assay for MERS diagnosis and for investigating the zoonotic reservoir, it uses live virus and so must be performed in high containment laboratories. Competitive ELISA (cELISA), in which a labeled monoclonal antibody (MAb) competes with test serum antibodies for target epitopes, may be a suitable alternative because it detects antibodies in a species-independent manner...
January 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/28978475/elicitation-of-neutralizing-antibodies-targeting-the-v2-apex-of-the-hiv-envelope-trimer-in-a-wild-type-animal-model
#19
James E Voss, Raiees Andrabi, Laura E McCoy, Natalia de Val, Roberta P Fuller, Terrence Messmer, Ching-Yao Su, Devin Sok, Salar N Khan, Fernando Garces, Laura K Pritchard, Richard T Wyatt, Andrew B Ward, Max Crispin, Ian A Wilson, Dennis R Burton
Recent efforts toward HIV vaccine development include the design of immunogens that can engage B cell receptors with the potential to affinity mature into broadly neutralizing antibodies (bnAbs). V2-apex bnAbs, which bind a protein-glycan region on HIV envelope glycoprotein (Env) trimer, are among the most broad and potent described. We show here that a rare "glycan hole" at the V2 apex is enriched in HIV isolates neutralized by inferred precursors of prototype V2-apex bnAbs. To investigate whether this feature could focus neutralizing responses onto the apex bnAb region, we immunized wild-type rabbits with soluble trimers adapted from these Envs...
October 3, 2017: Cell Reports
https://www.readbyqxmd.com/read/28958080/invasive-features-of-small-sized-lung-adenocarcinoma-adjoining-emphysematous-bullae
#20
Gouji Toyokawa, Mototsugu Shimokawa, Yuka Kozuma, Taichi Matsubara, Naoki Haratake, Shinkichi Takamori, Takaki Akamine, Kazuki Takada, Masakazu Katsura, Fumihiro Shoji, Tatsuro Okamoto, Yoshihiko Maehara
OBJECTIVES: Radiologically small-sized adenocarcinomas are special entities of lung cancer, as their radiological and pathological invasiveness determines the surgical procedures applied; however, the clinicopathological features of small-sized lung adenocarcinoma adjoining cystic airspaces (Ca-ADJ) have yet to be fully clarified. The aim of this study was to elucidate the clinicopathological characteristics, including the programmed death ligand 1 (PD-L1) expression, in patients with Ca-ADJ ≤3...
August 29, 2017: European Journal of Cardio-thoracic Surgery
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