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rabbit monoclonal antibody

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https://www.readbyqxmd.com/read/28640941/phox2b-reliably-distinguishes-neuroblastoma-among-small-round-blue-cell-tumors
#1
Yin P Hung, John P Lee, Andrew M Bellizzi, Jason L Hornick
AIMS: Neuroblastoma shows considerable histologic overlap with other small round blue cell tumors. PHOX2B, a transcription factor essential for autonomic nervous system development, has been reported as an immunohistochemical marker for neuroblastoma. The purpose of this study was to validate the specificity and diagnostic utility of PHOX2B for peripheral neuroblastic tumors. METHODS AND RESULTS: We evaluated 240 cases (133 in whole-tissue sections; 107 in tissue microarrays), including 76 peripheral neuroblastic tumors [median age 2 years; including 4 adults] and 164 other tumors: 44 Wilms tumors; 20 Ewing sarcomas; 10 each CIC-rearranged round cell sarcomas, poorly differentiated synovial sarcomas, lymphoblastic lymphomas, alveolar rhabdomyosarcomas, embryonal rhabdomyosarcomas, mesenchymal chondrosarcomas, Merkel cell carcinomas, olfactory neuroblastomas, and melanomas; 5 each NUT midline carcinomas and desmoplastic small round cell tumors...
June 22, 2017: Histopathology
https://www.readbyqxmd.com/read/28631595/a-32-kda-viral-attachment-protein-of-lymphocystis-disease-virus-lcdv-specifically-interacts-with-a-27-8-kda-cellular-receptor-from-flounder-paralichthys-olivaceus
#2
Ying Zhong, Chenjie Fei, Xiaoqian Tang, Wenbin Zhan, Xiuzhen Sheng
The 27.8 kDa protein in flounder gill (FG) cells was previously proved to be a receptor specific for lymphocystis disease virus (LCDV) entry and infection. In this paper, a 32 kDa viral attachment protein (VAP) of LCDV specifically binding to the 27.8 kDa receptor (27.8R) was found by far-Western blotting coupled with monoclonal antibodies (MAbs) against 27.8R. The 32 kDa protein was confirmed to be encoded by the open reading frame (ORF) 038 gene in LCDV-C, and predicted to contain a putative transmembrane region, multiple N-myristoylation and glycosylation sites and phosphorylation motifs...
June 20, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28627716/a-cherry-seed-derived-spice-mahleb-is-recognized-by-anti-almond-antibodies-including-almond-allergic-patient-ige
#3
Kyle A Noble, Changqi Liu, Shridhar K Sathe, Kenneth H Roux
There are a number of examples of immunologic cross-reactivity elicited by pollens, fruits, seeds, and nuts of closely related plant species. Such cross-reactivity is of particular concern for patients with food allergies. In this report, we investigated a spice (mahleb) that is prepared from the kernel of the St. Lucie cherry, Prunus mahaleb, for cross-reactivity with almond (Prunus dulcis), using enzyme-linked immunosorbent assay (ELISA) and Western blot. Almond and mahleb are members of the same genus. Cross-reactivity between the mahleb and almond was demonstrated by reaction of cherry and almond kernel protein extracts with antibodies raised against almond proteins...
June 19, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28621534/construction-of-an-immunized-rabbit-phage-display-library-for-selecting-high-activity-of-against-bacillus-thuringiensis-cry1f-toxin-single-chain-antibodies
#4
Chongxin Xu, Cunzheng Zhang, Jianfeng Zhong, Hui Hu, Shimin Luo, Xiaoqin Liu, Xiao Zhang, Yuan Liu, Xianjin Liu
In the present study, a Cry1F-immunized rabbit phage display library (6.96×108) was constructed for selecting high activity of anti-Cry1F toxin single-chain antibody (scFv) by bio-panning. Total 16 positive monoclonal phage scFvs were obtained after 4 rounds panning which identified by enzyme-linked immunoassay (ELISA), PCR and DNA sequencing. The most positive phage scFv (named RF4) was expressed in E.coli.HB2151 and the purified soluble protein approximate 30 kDa with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
June 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28596680/role-of-axl-in-invasion-and-drug-resistance-of-colon-and-breast-cancer-cells-and-its-association-with-p53-alterations
#5
Wael M Abdel-Rahman, Noura A Al-Khayyal, Vidhya A Nair, S R Aravind, Maha Saber-Ayad
AIM: To characterize AXL receptor tyrosine kinase (AXL) expression in relationship to tumor protein P53 (TP53 gene, p53 protein) and its role in tumor invasion and response to therapy. METHODS: We used 14 cell lines, including 3 isogenic pairs carrying mutant/knockout p53, to gain insight into the relationship between AXL and TP53. These included HCT116, HCT116.p53 mutant, RKO, and RKO.p53(-/-) lines (all from colon cancers) as well as breast cancer cell lines MCF7 and 1001 (MCF7-p53 mutant clone)...
May 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28577523/quantification-of-fel-d-1-in-house-dust-samples-of-cat-allergic-patients-by-using-monoclonal-antibody-specific-to-a-novel-ige-binding-epitope
#6
Natt Tasaniyananda, Anchalee Tungtrongchitr, Watee Seesuay, Yuwaporn Sakolvaree, Pisinee Aiumurai, Nitaya Indrawattana, Wanpen Chaicumpa, Nitat Sookrung
BACKGROUND: Avoidance of allergen exposure is an effective measure for preventing naÏve and allergic individuals from sensitization (primary intervention) and disease aggravation (secondary intervention), respectively. Regular monitoring of the allergens in the environment is required for the effective intervention. Thus, there is a need for cost-effective test kits for environmental allergen quantifications. OBJECTIVE: To invent a test kit for quantification of cat major allergen, Fel d 1...
June 1, 2017: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/28573869/obiltoxaximab-adding-to-the-treatment-arsenal-for-bacillus-anthracis-infection
#7
Audrey W Hou, Amanda M Morrill
OBJECTIVE: To review the safety and efficacy of obiltoxaximab, a monoclonal antibody indicated for the treatment of Bacillus anthracis inhalational anthrax in adult and pediatric patients. DATA SOURCES: A MEDLINE (1946 to May, week 1, 2017) and EMBASE (1980 to 2017, week 19) search was performed using the search terms obiltoxaximab OR ETI-204 OR Anthim AND anthrax. STUDY SELECTION AND DATA EXTRACTION: All English-language clinical studies in both animal and human models assessing the safety and efficacy of obiltoxaximab were included...
June 1, 2017: Annals of Pharmacotherapy
https://www.readbyqxmd.com/read/28542437/cp5-system-for-simple-and-highly-efficient-protein-purification-with-a-c-terminal-designed-mini-tag
#8
Hiroyuki Takeda, Wei Zhou, Kohki Kido, Ryoji Suno, Takahiro Iwasaki, Takuya Kobayashi, Tatsuya Sawasaki
There are many strategies to purify recombinant proteins of interest, and affinity purification utilizing monoclonal antibody that targets a linear epitope sequence is one of the essential techniques used in current biochemistry and structural biology. Here we introduce a new protein purification system using a very short CP5 tag. First, we selected anti-dopamine receptor D1 (DRD1) rabbit monoclonal antibody clone Ra62 (Ra62 antibody) as capture antibody, and identified its minimal epitope sequence as a 5-amino-acid sequence at C-terminal of DRD1 (GQHPT-COOH, D1CE sequence)...
2017: PloS One
https://www.readbyqxmd.com/read/28541149/characterization-of-equine-vitamin-d-binding-protein-development-of-an-assay-and-assessment-of-plasma-concentrations-of-the-protein-in-healthy-horses-and-horses-with-gastrointestinal-disease
#9
Tina H Pihl, Stine Jacobsen, Dorthe T Olsen, Peter Højrup, Astrid Grosche, David E Freeman, Pia H Andersen, Gunnar Houen
OBJECTIVE To purify and characterize equine vitamin D-binding protein (VDBP) from equine serum and to evaluate plasma concentrations of VDBP in healthy horses and horses with gastrointestinal injury or disease. ANIMALS 13 healthy laboratory animals (8 mice and 5 rabbits), 61 healthy horses, 12 horses with experimentally induced intestinal ischemia and reperfusion (IR), and 59 horses with acute gastrointestinal diseases. PROCEDURES VDBP was purified from serum of 2 healthy horses, and recombinant equine VDBP was obtained through a commercial service...
June 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28529510/advances-in-the-isolation-of-specific-monoclonal-rabbit-antibodies
#10
REVIEW
Zaibao Zhang, Huijuan Liu, Qian Guan, Lei Wang, Hongyu Yuan
The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28507619/expression-of-erg-protein-and-tmrpss2-erg-fusion-in-prostatic-carcinoma-in-egyptian-patients
#11
Ahmed Abdel-Hady, Ali El-Hindawi, Olfat Hammam, Heba Khalil, Sara Diab, Soulafa Abd El-Aziz, Mohamed Badawy, Ahmed Ismail, Noha Helmy, Nora Kamel, Shady Anis, Amr El Kholy, Khalid Al Osili, Afaf Abdel-Hady, Hani Nour, Maha Akl
AIM: Prostate cancer (PCa) is the second most common cancers in men worldwide. Its incidence can be influenced by several risk factors including genetic susceptibility. Therefore the search for the expression of a certain gene (ERG) and its rearrangement could give us clues for proper identification of PCa. And the study of ERG expression and its comparison to FISH in Egyptian patients can show whether ERG immunophenotype could be used instead of FISH, as it is cheaper. MATERIALS AND METHODS: This study was performed on 85 cases of PCa, showing 30 cases with HGPIN and 30 cases of prostatic hyperplasia...
April 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28505000/h3f3a-histone-3-3-g34w-immunohistochemistry-a-reliable-marker-defining-benign-and-malignant-giant-cell-tumor-of-bone
#12
Fernanda Amary, Fitim Berisha, Hongtao Ye, Manu Gupta, Alice Gutteridge, Daniel Baumhoer, Rebecca Gibbons, Roberto Tirabosco, Paul O'Donnell, Adrienne M Flanagan
Giant cell tumor of bone (GCTB) is a locally aggressive subarticular tumor. Having recently reported that H3.3 G34W mutations are characteristic of this tumor type, we have now investigated the sensitivity and specificity of the anti-histone H3.3 G34W rabbit monoclonal antibody in a wide variety of tumors including histologic mimics of GCTB to assess its value as a diagnostic marker. We also determined the incidence of H3.3 G34 mutations in primary malignant bone tumors as assessed by genotype and H3.3 G34W immunostaining...
May 12, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28487989/purification-of-a-polyclonal-antibody-against-cd147-for-elisa-using-antigen%C3%A2-immunoaffinity-chromatography
#13
Shuangshuang Liu, Shasha Li, Yang Zhang, Ye Wang, Yumeng Zhu, Bin Wang, Zhi-Nan Chen
The immunoglobulin superfamily member CD147 is a widely expressed glycoprotein that occurs in both a membrane‑spanning and soluble form. Sandwich ELISA is a powerful tool for analyzing soluble antigens. The aim of the present study was to obtain a highly specific polyclonal antibody against human CD147 that can be used for sandwich ELISA analysis. Expression of recombinant CD147 by a eukaryotic expression system was used to immunize rabbits to obtain antiserum. A highly specific polyclonal antibody that was able to detect soluble CD147 in sandwich ELISA was obtained by antigen‑immunoaffinity chromatography purification...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28414586/development-and-validation-of-monoclonal-antibody-based-antigen-capture-elisa-for-detection-of-group-a-porcine-rotavirus
#14
Atta Muhammad Memon, Anjuman Ara Bhuyan, Fangzhou Chen, Xiaozhen Guo, Harish Menghwar, Yinxing Zhu, Xugang Ku, Shuhua Chen, Zhonghua Li, Qigai He
Porcine rotavirus-A (PoRVA) is one of the common causes of mild to severe dehydrating diarrhea, leading to losses in weaning and postweaning piglets. A rapid, highly specific, and sensitive antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) was developed for detection of PoRVA, by using VP6 (a highly conserved and antigenic protein of group-A rotavirus)-directed rabbit polyclonal antibodies (capture antibody) and murine monoclonal antibodies (detector antibody). The detection limit of AC-ELISA was found to be equal to that of conventional reverse transcription-polymerase chain reaction (RT-PCR; about 10(2...
May 2017: Viral Immunology
https://www.readbyqxmd.com/read/28412300/mechanisms-of-retinal-damage-after-ocular-alkali-burns
#15
Eleftherios I Paschalis, Chengxin Zhou, Fengyang Lei, Nathan Scott, Vassiliki Kapoulea, Marie-Claude Robert, Demetrios Vavvas, Reza Dana, James Chodosh, Claes H Dohlman
Alkali burns to the eye constitute a leading cause of worldwide blindness. In recent case series, corneal transplantation revealed unexpected damage to the retina and optic nerve in chemically burned eyes. We investigated the physical, biochemical, and immunological components of retinal injury after alkali burn and explored a novel neuroprotective regimen suitable for prompt administration in emergency departments. Thus, in vivo pH, oxygen, and oxidation reduction measurements were performed in the anterior and posterior segment of mouse and rabbit eyes using implantable microsensors...
June 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28409324/generation-and-characterization-of-polyclonal-and-monoclonal-antibodies-to-human-ntpdase2-including-a-blocking-antibody
#16
Julie Pelletier, Hervé Agonsanou, Ninotchska Delvalle, Michel Fausther, Mabrouka Salem, Brian Gulbransen, Jean Sévigny
Nucleoside triphosphate diphosphohydrolase-2 (NTPDase2) is an ectonucleotidase that modulates P2 receptor activation by hydrolyzing ATP to ADP. In rodents, NTPDase2 is expressed by several specialized cell types such as vascular adventitial cells, neuroglial cells, hepatic portal fibroblasts, gustatory type I cells, and cells within the connective tissues of reproductive and gastrointestinal organs. Much less is known regarding the expression and function of NTPDase2 in humans. Here, we developed specific research tools to study human NTPDase2...
April 13, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28396686/expression-of-somatostatin-receptor-2-in-somatotropinoma-correlated-with-the-short-term-efficacy-of-somatostatin-analogues
#17
Wenjuan Liu, Lina Xie, Min He, Ming Shen, Jingjing Zhu, Yeping Yang, Meng Wang, Ji Hu, Hongying Ye, Yiming Li, Yao Zhao, Zhaoyun Zhang
The expression of somatostatin receptor subtypes (SSTRs) in pituitary growth hormone- (GH-) secreting adenomas may predict the response to somatostatin analogues (SSA). Our aim was to evaluate the value of the immunohistochemical (IHC) scores of 2 subtypes, SSTR2 and SSTR5, in predicting the short-term efficacy of SSA therapy in patients with active acromegaly. Ninety-three newly diagnosed acromegalic patients were included in our study. These patients were categorized into either a SSA-pretreated group (SA, n = 63) or a direct-surgery group (DS, n = 30), depending on whether or not presurgical SSA treatment was received...
2017: International Journal of Endocrinology
https://www.readbyqxmd.com/read/28394698/generation-of-therapeutic-immunoconjugates-via-residue-specific-conjugation-technology-respect-utilizing-a-native-cysteine-in-the-light-chain-framework-of-oryctolagus-cuniculus
#18
Earl F Albone, Jared L Spidel, Xin Cheng, Young Chul Park, Sara Jacob, Andrew Z Milinichik, Ben Vassen, Janet Butler, J Bradford Kline, Luigi Grasso
The conjugation of toxins, dyes, peptides, or proteins to monoclonal antibodies is often performed via free thiol groups generated by either partial reduction methods or engineering free cysteine residues into the antibody sequence. Antibodies from the rabbit Oryctolagus cuniculus have an additional intrachain disulfide bond, whereby the light chain variable kappa domain is bridged to the constant kappa region between cysteine residues at positions 80 and 171, respectively. Chimerization of rabbit antibodies with human constant domains allows for the generation of a free thiol group at the light chain position 80 (C80) that can be used for site-specific conjugation...
April 10, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28389319/in-vitro-immunoregulatory-effects-of-thymoglobulin-on-human-immune-cell-subpopulations
#19
Maja Buszko, Bojana Jakic, Christian Ploner, Paul Hoertnagl, Christina Mayerl, Georg Wick, Giuseppe Cappellano
Thymoglobulin (ATG) is a polyclonal rabbit antibody against human thymocytes used as a T cell-depleting agent in organ transplantation. Its polyclonal character suggests that its effect may go far beyond just T cell depletion. The aim of this study was to further elucidate possible mechanisms underlying the suppressive activity of ATG. For in vitro studies, human peripheral blood mononuclear cells (PBMC) were incubated with ATG or control Ig for various time points. Foxp3(+) regulatory cells (Tregs) and monocytes were phenotypically analyzed by flow cytometry and functionally tested by in vitro suppression assays...
April 4, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28387612/development-of-mpmab-1-a-mouse-rat-chimeric-antibody-against-mouse-podoplanin
#20
Shinji Yamada, Mika K Kaneko, Takuro Nakamura, Osamu Ichii, Satoru Konnai, Yukinari Kato
Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb...
April 7, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
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