keyword
MENU ▼
Read by QxMD icon Read
search

rabbit monoclonal antibody

keyword
https://www.readbyqxmd.com/read/29627856/generation-and-characterization-of-a-novel-recombinant-scfv-antibody-specific-for-campylobacter-jejuni
#1
Ruramayi M Nzuma, Fuquan Liu, Irene R Grant
Campylobacter jejuni is a leading cause of foodborne illness worldwide, mainly due to consumption and handling of contaminated raw chicken. Rapid detection methods for C. jejuni are vital for monitoring contamination levels in chicken products and reducing human Campylobacteriosis cases. The 'gold standard' culture-based method of Campylobacter detection takes 3-5 days and is too slow to permit effective intervention. Immuno-based methods are faster, but usually necessitate use of animals or hybridoma technology to produce antibodies; making them difficult and expensive to produce...
April 7, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29563917/strategies-for-generating-diverse-antibody-repertoires-using-transgenic-animals-expressing-human-antibodies
#2
Weihsu C Chen, Christopher M Murawsky
Therapeutic molecules derived from antibodies have become a dominant class of drugs used to treat human disease. Increasingly, therapeutic antibodies are discovered using transgenic animal systems that have been engineered to express human antibodies. While the engineering details differ, these platforms share the ability to raise an immune response that is comprised of antibodies with fully human idiotypes. Although the predominant transgenic host species has been mouse, the genomes of rats, rabbits, chickens, and cows have also been modified to express human antibodies...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29522252/rapid-multiple-immunofluorescent-staining-for-the-simultaneous-detection-of-cytokeratin-and-vimentin-in-the-cytology-of-canine-tumors
#3
Mariko Sawa, Akira Yabuki, Moeko Kohyama, Noriaki Miyoshi, Osamu Yamato
BACKGROUND: Immunocytochemistry (ICC) is utilized as an advanced technique in veterinary cytology. In tumor diagnosis, cytokeratin and vimentin are markers used to distinguish the origin of tumor cells. Standard enzyme-based ICC has limitations in clinical use; and therefore, more convenient and reliable methods are needed. OBJECTIVES: The purpose of this study was to develop a rapid multiple immunofluorescent (RMIF) detection method for dual cytokeratin and vimentin staining on cytology slides in dogs...
March 9, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/29508480/identification-of-metastatic-papillary-thyroid-carcinoma-in-fna-specimens-using-thyroid-peroxidase-immunohistochemistry
#4
P W Shield, S J Crouch, D J Papadimos, M D Walsh
INTRODUCTION: We evaluated immunohistochemical staining for thyroid peroxidase (TPO), a glycoprotein found in the apical plasma membrane of thyroid follicular cells, as a marker for metastatic PTC in FNA samples and compared results with thyroglobulin (Tg) and thyroid transcription factor 1 (TTF1) staining. METHODS: Cell block sections prepared from 100 FNA specimens were stained with a rabbit monoclonal antibody to TPO (EP159). The FNAs included 64 metastatic malignancies from non-thyroid primary sites, including 18 lung, and 36 cases of thyroid tumours (29 PTC, six cases of medullary thyroid carcinoma and one thyroid anaplastic carcinoma)...
March 6, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/29504532/generation-and-partial-characterization-of-rabbit-monoclonal-antibody-to-pyroglutamate-amyloid-%C3%AE-3-42-pe3-a%C3%AE
#5
Pankaj D Mehta, Bruce A Patrick, Marc Barshatzky, Sangita P Mehta, Janusz Frackowiak, Bozena Mazur-Kolecka, Jerzy Wegiel, Thomas Wisniewski, David L Miller
N-terminally truncated pyroglutamate amyloid-β (Aβ) peptide starting at position 3 represents a significant fraction of Aβ peptides (pE3-Aβ) in amyloid plaques of postmortem brains from patients with Alzheimer's disease (AD) and older persons with Down syndrome (DS). Studies in transgenic mouse models of AD also showed that pE3-Aβ is a major component of plaques, and mouse monoclonal antibody to pE3-Aβ appears to be a desirable therapeutic agent for AD. Since small peptides do not typically elicit a good immune response in mice, but do so favorably in rabbits, our aims were to generate and partially characterize a rabbit monoclonal antibody (RabmAb) to pE3-Aβ...
August 22, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29500044/immunodominance-of-lipl32-93-272-peptides-revealed-by-leptospirosis-sera-and-therapeutic-monoclonal-antibodies
#6
Tippawan Pissawong, Santi Maneewatchararangsri, Nonglucksanawan Ritthisunthorn, Ngamphol Soonthornworasiri, Onrapak Reamtong, Poom Adisakwattana, Thareerat Kalambaheti, Urai Chaisri, Galayanee Doungchawee
BACKGROUND/PURPOSE: Leptospirosis is a neglected zoonosis, imposing significant human and veterinary public health burdens. In this study, recombinant LipL3293-147 and LipL32148-184 middle domain of LipL3293-184 , and LipL32171-214 , and LipL32215-272 of c-terminal LipL32171-272 truncations were defined for immunodominance of the molecule during Leptospira infections revealed by leptospirosis sera. RESULTS: IgM-dominant was directed to highly surface accessible LipL32148-184 and Lipl32171-214 ...
February 14, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29490698/clinical-evaluation-of-haploidentical-hematopoietic-combined-with-human-umbilical-cord-derived-mesenchymal-stem-cells-in-severe-aplastic-anemia
#7
Lixin Xu, Zhouyang Liu, Yamei Wu, Xueliang Yang, Yongbin Cao, Xiaohong Li, Bei Yan, Songwei Li, Wanming Da, Xiaoxiong Wu
BACKGROUND: This study not only evaluated the clinical effects of treatment using haploidentical hematopoietic stem cells (haplo-HSCs) combined with human umbilical cord mesenchymal stem cells (UC-MSCs) in patients with severe aplastic anemia (SAA), but also investigated the factors related to graft versus host disease (GVHD). METHODS: Cotransplantation of haplo-HSCs and UC-MSCs was performed in 24 SAA patients. The conditioning regimens consisted of rabbit anti-human T-lymphocyte immunoglobulin (ATG), cyclophosphamide, and fludarabine with or without busulfan...
March 1, 2018: European Journal of Medical Research
https://www.readbyqxmd.com/read/29460891/-the-proliferative-activity-of-tumor-cells-and-the-distribution-of-sox2-pluripotent-stem-cells-in-various-histological-types-of-ameloblastoma
#8
I I Babichenko, V F Rybalskaya, N S Tsimbalist, V A Semkin
AIM: to investigate the proliferative activity and distribution of pluripotent stem cells in various histological types of amelobrastoma. MATERIAL AND METHODS: The surgical and archival materials of 76 patients with ameloblastoma from the Central Research Institute of Dentistry and Oral Surgery, Ministry of Health of the Russian Federation, were used. Tissue antigens were determined using a rabbit monoclonal antibody against to Ki-67 and SOX2. RESULTS: Proliferating (Ki-67) and pluripotent (SOX2) cells were identified in various histological types of ameloblastoma...
2018: Arkhiv Patologii
https://www.readbyqxmd.com/read/29444803/open-source-recombinant-monoclonal-secondary-nanobodies
#9
Helge Ewers
Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays.
February 14, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29442712/development-of-a-colloidal-gold-based-immunochromatographic-assay-for-the-rapid-detection-of-edwardsiella-ictaluri
#10
Ruijun Li, Xianyu Zhang, Chuan Yang, Shigen Ye, Hua Li, Qiang Li
Edwardsiella Ictaluri is known as the etiological agent of enteric septicaemia of channel catfish, causing heavy economic losses in the aquaculture industry. In this study, a colloidal gold-based immunochromatography assay (GICA) was developed for rapid detection of E. ictaluri. Briefly, monoclonal antibody (MAbs) and polyclonal antibody (PAbs) against E. ictaluri were prepared. Sensitivity of MAbs and PAbs to E. ictaluri was analyzed by Dot ELISA. Mouse MAb5D11 against E. ictaluri was conjugated with the 20 nm colloidal gold particles as the detector...
June 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29383151/high-protein-and-mrna-expression-levels-of-tubb3-class-iii-%C3%A3-tubulin-are-associated-with-aggressive-tumor-features-in-esophageal-adenocarcinomas
#11
Heike Loeser, Simon Schallenberg, Moritz von Winterfeld, Lars Tharun, Hakan Alakus, Arnulf Hölscher, Elfriede Bollschweiler, Reinhard Buettner, Thomas Zander, Alexander Quaas
Background: Esophageal adenocarcinomas show an increasing incidence in the Western world and their overall survival remains low. Microtubules are multifunctional cytoskeletal proteins involved in crucial cellular roles, including maintenance of cell shape, intracellular transport, meiosis, and mitosis. Microtubulus-TUBB3 was found overexpressed in several carcinomas suggesting a significant role in cancer development. High levels of TUBB3 expression were also described to be associated with poor clinical outcome in various cancers...
December 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29378261/improving-the-quality-of-a-recombinant-rabbit-monoclonal-antibody-against-plxdc2-by-optimizing-transient-expression-conditions-and-purification-method
#12
Hisayo Shimizu, Masataka Nakagawa, Nemuri Todaka, Keitaro Imaizumi, Yasunori Kurosawa, Toshiaki Maruyama, C J Okumura, Takashi Shibata, Yosuke Tanaka, Yoshinori Sato, Yasuo Ono, Teruo Akuta
Rabbit monoclonal antibodies (mAbs) have many advantages over mouse antibodies in biological research and diagnostics applications because they exhibit high affinity and specificity. However, the methods of recombinant rabbit mAb production have not been optimized to the same extent as techniques used to produce mouse and human mAbs. In this study, we sought to optimize the production of a recombinant rabbit mAb against human plexin domain containing protein 2 (PLXDC2), a known cell surface antigen, by culturing HEK293-6E cells transfected with antibody-encoding genes at two different temperatures and by purifying the end-product by three different chromatography methods...
January 26, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29343576/increased-epitope-complexity-correlated-with-antibody-affinity-maturation-and-a-novel-binding-mode-revealed-by-structures-of-rabbit-antibodies-against-the-third-variable-loop-v3-of-hiv-1-gp120
#13
Ruimin Pan, Yali Qin, Marisa Banasik, William Lees, Adrian J Shepherd, Michael W Cho, Xiang-Peng Kong
The V3 loop of HIV-1 gp120 is an immunodominant region targeted by neutralizing antibodies (nAbs). Despite limited breadth, better characterization of the structural details of the interactions between these nAbs and their target epitopes would enhance our understanding of the mechanism of neutralization and facilitate designing better immunogens to induce nAbs with greater breadth. Recently, we isolated two anti-V3 neutralizing monoclonal antibodies (mAbs), 10A3 and 10A37, from a rabbit immunized with gp120 of the M group consensus sequence...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29338152/generation-and-characterization-of-siglec-f-specific-monoclonal-antibodies
#14
Sima Shahmohammadi-Farid, Roya Ghods, Mahmood Jeddi-Tehrani, Ali-Ahmad Bayat, Nazanin Mojtabavi, Alireza Razavi, Amir-Hassan Zarnani
Siglec-F (SF) is a surface glycoprotein expressed by mouse eosinophils and induces caspase- and mitochondria-dependent apoptosis after engagement with its cognate ligand or specific antibodies. This targeting eosinophils by monoclonal antibodies may help diverse diseases associated with increased frequency of eosinophils including allergy and asthma. In this paper, production of murine and rat monoclonal antibodies (mAbs) against Siglec-F has been addressed. Balb/c mice were immunized with siglec-F1 (SF1) and siglec-F2 (SF2) synthetic peptides conjugated to a carrier protein...
December 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29324051/anti-il-8-antibody-potentiates-the-effect-of-exogenous-surfactant-in-respiratory-failure-caused-by-meconium-aspiration
#15
Pavol Mikolka, Jana Kopincova, Petra Kosutova, Maros Kolomaznik, Andrea Calkovska, Daniela Mokra
AIM: Meconium aspiration syndrome (MAS) is life-threatening respiratory failure of newborns which can be treated by exogenous surfactant. In response to meconium, increased levels of chemokine IL-8 (CXCL8) stimulate massive neutrophil infiltration of the lungs. Local accumulation and activation of neutrophils, on-going inflammation, lung edema, and oxidative damage contribute to inactivation of endogenous and therapeutically given surfactants. Therefore, we have hypothesized that addition of monoclonal anti-IL-8 antibody into exogenous surfactant can mitigate the neutrophil-induced local injury and the secondary surfactant inactivation and may finally result in improvement of respiratory functions...
February 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29207829/evaluation-of-tumour-associated-macrophages-and-angiogenesis-in-ameloblastoma
#16
Akinyele O Adisa, Samuel E Udeabor, Anna Orlowska, Robert A Sader, Shahram Ghanaati
Introduction: Ameloblastoma is a locally invasive odontogenic neoplasm that has a high recurrence rate. The invasion of adjacent tissue is supported by angiogenesis stimulated by Tumour-Associated Macrophages (TAMs). TAMs are macrophages modified in the milieu of the tumour microenvironment and have very weak or no ability to present antigens. Thus, there is collaboration between the tumour and the tumour microenvironment to maintain tumour enlargement. TAMs exist as the classically activated M1 macrophages that possess antitumour activity and the otherwise activated M2 macrophages that support tumour invasion and metastasis...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29205549/identification-and-characterization-of-antibodies-elicited-by-human-cystatin-c-fragment
#17
Izabela Behrendt, Martyna Prądzińska, Marta Spodzieja, Paulina Czaplewska, Aleksandra S Kołodziejczyk, Aneta Szymańska, Franciszek Kasprzykowski, Susanna L Lundström, Roman A Zubarev, Sylwia Rodziewicz-Motowidło
Amyloid formation is associated with a number of neurodegenerative diseases that affect the independence and quality of life of aging populations. One of rather atypical, occurring at a young age amyloidosis is hereditary cystatin C amyloid angiopathy (HCCAA) related to aggregation of L68Q variant of human cystatin C (hCC). Human cystatin C plays a very important role in many aspects of human health; however, its amyloidogenic properties manifested in HCCAA present a real, lethal threat to some populations and any work on factors that can affect possible influencing hCC aggregation is not to overestimate...
December 5, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29199679/a-comparative-analysis-of-immunohistochemistry-and-fluorescent-in-situ-hybridization-assay-to-detect-anaplastic-lymphoma-kinase-status-in-lung-adenocarcinoma-cases-a-search-for-a-testing-algorithm
#18
P B Wagle, N A Jambhekar, R Kumar, K Prabhash, C S Pramesh, S B Desai, V Noronha, G Karimundackal, A Shah, A Joshi, S G Laskar, S Jiwnani, T Pai, J P Agarwal
INTRODUCTION: Testing for echinoderm microtubule-associated protein-like 4 (EML4) anaplastic lymphoma kinase (ALK) translocation by fluorescence in situ hybridization (FISH) is well established whereas the Food and Drug Administration (FDA) ALK immunohistochemical (IHC) test is relatively new. AIMS AND OBJECTIVE: The aim of this study is to compare FDA-approved ALK IHC test (D5F3 clone) with the standard ALK FISH test. MATERIALS AND METHODS: A validation and a test arm with 100 and 200 cases of Formalin-Fixed, Paraffin-embedded blocks of lung adenocarcinoma, respectively, comprised the material...
January 2017: Indian Journal of Cancer
https://www.readbyqxmd.com/read/29187883/programmed-cell-death-ligand-1-protein-levels-predicted-survival-of-non-small-cell-lung-cancer
#19
Shaohua Cui, Xinying Su, Lili Dong, Jialin Qian, Lin Ye, Tianwei Zhang, Haihua Fu, Hulin Han, Jiaqi Huang, Yihong Yao, Yi Gu, Liyan Jiang
Objective: To investigate the relationship between programmed death ligand 1 (PD-L1) expression using 5%, 25%, 50% cutoffs in tumor cells (TC) and postsurgical survival in non-small-cell lung cancer (NSCLC) patients. For samples with tumor infiltrating lymphocytes (TIL), correlation between PD-L1 expression in TIL using 1% cutoff and postsurgical survival was also evaluated. Methods: Primary NSCLC tumor surgical samples staging I to IIIA of 126 patients who underwent surgical procedures from September 2009 to August 2012 in Shanghai Chest Hospital, Shanghai Jiao Tong University were retrospectively included...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29187698/evaluation-of-schistosoma-japonicum-thioredoxin-peroxidase-1-as-a-potential-circulating-antigen-target-for-the-diagnosis-of-asian-schistosomiasis
#20
Adrian Miki C Macalanda, Jose Ma M Angeles, Kharleezelle J Moendeg, Anh Tm Dang, Luna Higuchi, Noboru Inoue, Xuenan Xuan, Masashi Kirinoki, Yuichi Chigusa, Lydia R Leonardo, Elena A Villacorte, Pilarita T Rivera, Yasuyuki Goto, Shin-Ichiro Kawazu
Asian schistosomiasis caused by Schistosoma japonicum is a serious zoonotic disease endemic in China, the Philippines and parts of Indonesia. Mass drug administration in endemic areas resulted to decline in disease severity and intensity. The low intensity of infection limits the use of current parasitological methods for schistosomiasis diagnosis. Detection of parasite circulating antigens might provide more informative result as it may indicate the true status of infection. In this study, S. japonicum thioredoxin peroxidase-1 (SjTPx-1) a 22 kDa secreted antioxidant enzyme expressed throughout the life stages of the parasite was evaluated for its potential use as a biomarker for schistosomiasis japonica infection...
February 2, 2018: Journal of Veterinary Medical Science
keyword
keyword
93912
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"