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rabbit monoclonal antibody

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https://www.readbyqxmd.com/read/29888794/sensitivity-and-specificity-of-phospho-ser129-%C3%AE-synuclein-monoclonal-antibodies
#1
Vedad Delic, Sidhanth Chandra, Hisham Abdelmotilib, Tyler Maltbie, Shijie Wang, Danielle Kem, Hunter J Scott, Rachel N Underwood, Zhiyong Liu, Laura A Volpicelli-Daley, Andrew B West
α-Synuclein (α-syn) is an abundant presynaptic protein that is the primary constituent of inclusions that define Lewy body diseases (LBDs). In these inclusions, α-syn is phosphorylated at the serine-129 residue. Antibodies directed to this phosphorylation site are used to measure inclusion abundance and stage disease progression in pre-clinical models as well as in post-mortem tissues in LBDs. While it is critical to reliably identify inclusions, phospho-specific antibodies often cross-react with non-specific antigens...
June 11, 2018: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29888023/absence-of-rejection-in-a-facial-allograft-recipient-with-a-positive-flow-crossmatch-24-months-after-induction-with-rabbit-anti-thymocyte-globulin-and-anti-cd20-monoclonal-antibody
#2
Bruce E Gelb, J Rodrigo Diaz-Siso, Natalie M Plana, Adam Jacoby, William J Rifkin, Kimberly S Khouri, Daniel J Ceradini, Eduardo D Rodriguez
Background: Donor-specific antibodies (DSA) to human leukocyte antigen increase the risk of accelerated rejection and allograft damage and reduce the likelihood of successful transplantation. Patients with full-thickness facial burns may benefit from facial allotransplantation. However, they are at a high risk of developing DSA due to standard features of their acute care. Case Presentation: A 41-year-old male with severe disfigurement from facial burns consented to facial allotransplantation in 2014; panel reactive antibody score was 0%...
2018: Case Reports in Transplantation
https://www.readbyqxmd.com/read/29882300/critical-assessment-of-the-efficiency-of-cd34-and-cd133-antibodies-for-enrichment-of-rabbit-hematopoietic-stem-cells
#3
Jaromír Vašíček, Medhat Shehata, Susanne Schnabl, Martin Hilgarth, Rainer Hubmann, Ulrich Jäger, Miroslav Bauer, Peter Chrenek
Rabbits have many hereditary diseases common to humans and are therefore a valuable model for regenerative disease and hematopoietic stem cell (HSC) therapies. Currently, there is no substantial data on the isolation and/or enrichment of rabbit HSCs. This study was initiated to evaluate the efficiency of the commercially available anti-CD34 and anti-CD133 antibodies for the detection and potential enrichment of rabbit HSCs from peripheral blood. PBMCs from rabbit and human blood were labelled with different clones of anti-human CD34 monoclonal antibodies (AC136, 581 and 8G12) and rabbit polyclonal CD34 antibody (pCD34) and anti-human CD133 monoclonal antibodies (AC133 and 293C3)...
June 8, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29867859/development-of-colloidal-gold-based-immunochromatographic-assay-for-rapid-detection-of-goose-parvovirus
#4
Xianglong Yu, Lei Wei, Hao Chen, Xiaoyu Niu, Yanguo Dou, Jing Yang, Zhenzhong Wang, Yi Tang, Youxiang Diao
Goose parvovirus (GPV) remains as a worldwide problem in goose industry. For this reason, it is necessary to develop a new diagnostic approach that is easier and faster than conventional tests. A rapid immunochromatographic assay based on antibody colloidal gold nanoparticles specific to GPV was developed for the detection of GPV in goose allantoic fluid and supernatant of tissue homogenate. The monoclonal antibodies (Mab) was produced by immunizing the BALB/c mice with purified GPV suspension, and the polyclonal antibody (pAb) was produced by immunizing the rabbits with recombinant VP3 protein...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29863809/evaluation-of-pan-trk-immunohistochemistry-in-infantile-fibrosarcoma-lipofibromatosis-like-neural-tumor-and-histologic-mimics
#5
Yin P Hung, Christopher D M Fletcher, Jason L Hornick
AIMS: Infantile fibrosarcoma is characterized by intersecting fascicles of spindle cells and ETV6-NTRK3 gene fusion in most cases. Given histologic overlap with other spindle-cell tumors, the diagnosis can be challenging and often requires molecular confirmation. A recently developed pan-TRK antibody shows promise for identifying tumors with NTRK fusions. The purpose of this study was to evaluate the potential diagnostic utility of pan-TRK immunohistochemistry for infantile fibrosarcoma...
June 4, 2018: Histopathology
https://www.readbyqxmd.com/read/29860455/anti-cd160-alone-or-in-combination-with-bevacizumab-is-a-potent-inhibitor-of-ocular-neovascularization-in-rabbit-and-monkey-models
#6
Thierry Menguy, Anne Briaux, Elisabeth Jeunesse, Jérôme Giustiniani, Alexandre Calcei, Thierry Guyon, Jacques Mizrahi, Hélène Haegel, Vanessa Duong, Vincent Soler, Pierre Brousset, Armand Bensussan, Isabelle Raymond Letron, Philippe Le Bouteiller
Purpose: To assess the efficacy of the murine first-in-class CL1-R2 monoclonal antibody (mAb) targeting human CD160 (alone or in combination with bevacizumab) by using the rabbit corneal neovascularization (CNV) model, and determine the safety and efficacy of ELB01101, a novel CL1-R2-derived humanized IgG4 mAb, in a monkey model of choroidal neovascularization (ChNV). Methods: Comparison of effect of CL1-R2, bevacizumab, or aflibercept or IgG1 (control) injections in early and late treatment schemes on evolution of VEGF- or FGF2-induced rabbit CNV was performed...
June 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29845491/inhibition-of-tissue-factor-pathway-inhibitor-tfpi-as-a-treatment-for-haemophilia-rationale-with-focus-on-concizumab
#7
Pratima Chowdary
Replacement therapy with missing factor (F) VIII or IX in haemophilia patients for bleed management and preventative treatment or prophylaxis is standard of care. Restoration of thrombin generation through novel mechanisms has become the focus of innovation to overcome limitations imposed by protein replacement therapy. Tissue factor pathway inhibitor (TFPI) is a multivalent Kunitz-type serine protease inhibitor that regulates tissue factor (TF)-induced coagulation through a FXa-dependent feedback inhibition of the TF...
May 29, 2018: Drugs
https://www.readbyqxmd.com/read/29843069/evaluation-of-cmet-aberration-by-immunohistochemistry-and-fluorescence-in-situ-hybridization-fish-in-triple-negative-breast-cancers
#8
REVIEW
Mopei Wang, Li Liang, Xiudong Lei, Asha Multani, Funda Meric-Bernstam, Debasish Tripathy, Yun Wu, Hui Chen, Hong Zhang
PURPOSE: To evaluate the incidence of cMET proto-oncogene aberration in a cohort of triple negative breast cancers using immunohistochemistry and fluorescence in situ hybridization (FISH) methods and correlated with patient outcome. PATIENTS AND METHODS: One hundred and six female patients with diagnosis of triple negative invasive breast carcinoma at The University of Texas-M D Anderson Cancer Center from 1983 to 2009 were included in the study. Expression of cMET was assessed by IHC using rabbit monoclonal anti-total cMET antibody (SP44 from Ventana)...
May 3, 2018: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29803557/regulation-of-corneal-noradrenaline-release-and-topography-of-sympathetic-innervation-functional-implications-for-adrenergic-mechanisms-in-the-human-cornea
#9
Luis Figueira, Catarina Janeiro, Fatima Ferreirinha, Paula Serrao, Sara Perestrelo, Fernando Falcao-Reis, Paulo Correia-de-Sa, Daniel Moura
Having established a main neuronal origin for noradrenaline (NA) in the cornea, we set out to study the physiologic determinants of its release and to correlate functional findings with sympathetic nerve density and overall topography. Whole corneas were obtained from 3 to 4 month-old rabbits and human donors. Study of prejunctional effects was carried out after incubation with radiolabelled NA (3H-NA). Corneas were superfused with warm aerated amine-free medium with cocaine and hydrocortisone to block subsequent neuronal and extraneuronal NA uptake...
May 24, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29795099/-the-role-of-wnt-%C3%AE-catenin-signaling-pathway-in-ameloblastoma-formation
#10
I I Babichenko, N S Tsimbalist, V F Rybal'skaya, A A Sherstnev, V A Syomkin
The aim of the study was to assess the role of the canonical Wnt/β-catenin signaling pathway in the formation and clinical course of various histological variants of ameloblastoma. MATERIAL AND METHODS: 76 cases of ameloblastoma were investigated, the average age of the patients was 46 years. There were 49 (64.5%) cases of recurrence of the disease. Tissue antigens were determined using monoclonal rabbit antibodies to Ki-67 and β-catenin. RESULTS: it was found correlation between the proliferative activity of ameloblastoma cells and the intranuclear localization of β-catenin, indicating the activation of the Wnt/β-catenin-signaling pathway...
2018: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29789565/the-wide-utility-of-rabbits-as-models-of-human-diseases
#11
REVIEW
Pedro J Esteves, Joana Abrantes, Hanna-Mari Baldauf, Lbachir BenMohamed, Yuxing Chen, Neil Christensen, Javier González-Gallego, Lorenzo Giacani, Jiafen Hu, Gilla Kaplan, Oliver T Keppler, Katherine L Knight, Xiang-Peng Kong, Dennis K Lanning, Jacques Le Pendu, Ana Lemos de Matos, Jia Liu, Shuying Liu, Ana M Lopes, Shan Lu, Sheila Lukehart, Yukari C Manabe, Fabiana Neves, Grant McFadden, Ruimin Pan, Xuwen Peng, Patricia de Sousa-Pereira, Ana Pinheiro, Masmudur Rahman, Natalie Ruvoën-Clouet, Selvakumar Subbian, Maria Jesús Tuñón, Wessel van der Loo, Michael Vaine, Laura E Via, Shixia Wang, Rose Mage
Studies using the European rabbit Oryctolagus cuniculus contributed to elucidating numerous fundamental aspects of antibody structure and diversification mechanisms and continue to be valuable for the development and testing of therapeutic humanized polyclonal and monoclonal antibodies. Additionally, during the last two decades, the use of the European rabbit as an animal model has been increasingly extended to many human diseases. This review documents the continuing wide utility of the rabbit as a reliable disease model for development of therapeutics and vaccines and studies of the cellular and molecular mechanisms underlying many human diseases...
May 22, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29784859/igg-responses-to-the-plasmodium-falciparum-antigen-var2csa-in-colombia-are-restricted-to-pregnancy-and-are-not-induced-by-exposure-to-p-vivax
#12
Mary Lopez-Perez, Mads Delbo Larsen, Rafael Bayarri-Olmos, Paulina Ampomah, Liz Stevenson, Myriam Arévalo-Herrera, Sócrates Herrera, Lars Hviid
Clinical immunity to malaria is associated with acquisition of IgG specific for members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family of clonally variant antigens on the surface of infected erythrocytes (IEs). The VAR2CSA subtype of PfEMP1 mediates IE binding in the placenta. VAR2CSA-specific IgG is normally acquired only after exposure to placental parasites. However, it was recently reported that men and children from Colombia often have high levels of functional VAR2CSA-specific IgG...
May 21, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29736673/development-and-evaluation-of-a-gold-nanoparticle-based-immunochromatographic-strip-test-for-the-detection-of-canine-parvovirus
#13
Chhavi Sharma, Mithilesh Singh, Vikramaditya Upmanyu, Vishal Chander, Suman Verma, Soumendu Chakrovarty, Gaurav K Sharma, Himani Dhanze, Praveen Singh, Sameer Shrivastava, Jyoti Kumar, Tapas Kumar Goswami, V K Gupta
Canine parvovirus (CPV) is the leading viral cause of enteritis in dogs and occurs mainly in 6- to 8-week-old pups. Rapid diagnosis of CPV under field conditions is now possible due to commercially available immunochromatographic (IC) assays. However, these commercial kits are somewhat expensive because they utilize a minimum of two monoclonal antibodies (mAbs) targeting different epitopes as capture and detector antibodies. Using only a single mAb for both capture and detection purpose may reduce the sensitivity of the assay...
May 8, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29736037/broadly-neutralizing-antibodies-from-human-survivors-target-a-conserved-site-in-the-ebola-virus-glycoprotein-hr2-mper-region
#14
Andrew I Flyak, Natalia Kuzmina, Charles D Murin, Christopher Bryan, Edgar Davidson, Pavlo Gilchuk, Christopher P Gulka, Philipp A Ilinykh, Xiaoli Shen, Kai Huang, Palaniappan Ramanathan, Hannah Turner, Marnie L Fusco, Rebecca Lampley, Nurgun Kose, Hannah King, Gopal Sapparapu, Benjamin J Doranz, Thomas G Ksiazek, David W Wright, Erica Ollmann Saphire, Andrew B Ward, Alexander Bukreyev, James E Crowe
Ebola virus (EBOV) in humans causes a severe illness with high mortality rates. Several strategies have been developed in the past to treat EBOV infection, including the antibody cocktail ZMapp, which has been shown to be effective in nonhuman primate models of infection 1 and has been used under compassionate-treatment protocols in humans 2 . ZMapp is a mixture of three chimerized murine monoclonal antibodies (mAbs)3-6 that target EBOV-specific epitopes on the surface glycoprotein7,8 . However, ZMapp mAbs do not neutralize other species from the genus Ebolavirus, such as Bundibugyo virus (BDBV), Reston virus (RESTV) or Sudan virus (SUDV)...
May 7, 2018: Nature Microbiology
https://www.readbyqxmd.com/read/29733313/an-hs-mrm-assay-for-the-quantification-of-host-cell-proteins-in-protein-biopharmaceuticals-by-liquid-chromatography-ion-mobility-qtof-mass-spectrometry
#15
Catalin Doneanu, Jing Fang, Yun Alelyunas, Ying Qing Yu, Mark Wrona, Weibin Chen
The analysis of low-level (1-100 ppm) protein impurities (e.g., host-cell proteins (HCPs)) in protein biotherapeutics is a challenging assay requiring high sensitivity and a wide dynamic range. Mass spectrometry-based quantification assays for proteins typically involve protein digestion followed by the selective reaction monitoring/multiple reaction monitoring (SRM/MRM) quantification of peptides using a low-resolution (Rs ~1,000) tandem quadrupole mass spectrometer. One of the limitations of this approach is the interference phenomenon observed when the peptide of interest has the "same" precursor and fragment mass (in terms of m/z values) as other co-eluting peptides present in the sample (within a 1-Da window)...
April 17, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29715580/expression-of-p27-and-c-myc-by-immunohistochemistry-in-breast-ductal-cancers-in-african-american-women
#16
Farhan Khan, Luisel J Ricks-Santi, Rabia Zafar, Yasmine Kanaan, Tammey Naab
OBJECTIVES: Proteins p27 and c-Myc are both key players in the cell cycle. While p27, a tumor suppressor, inhibits progression from G1 to S phase, c-Myc, a proto-oncogene, plays a key role in cell cycle regulation and apoptosis. The objective of our study was to determine the association between expression of c-Myc and the loss of p27 by immunohistochemistry (IHC) in the four major subtypes of breast cancer (BC) (Luminal A, Luminal B, HER2, and Triple Negative) and with other clinicopathological factors in a population of 202 African-American (AA) women...
April 5, 2018: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29709424/preclinical-pharmacokinetics-of-a-recombinant-humanized-rabbit-anti-vegf-monoclonal-antibody-in-rabbits-and-monkeys
#17
Dong-An Yu, Meng You, Wan-Wan Ji, You Lu, Bin Liu, Shou-Sheng Yan
TMAB001 is a humanized rabbit monoclonal antibody (mAb) designed to bind and neutralize human vascular endothelial growth factor (VEGF)-165. The purpose of the study was to investigate the pharmacokinetics (PK) and ocular tissue distribution after a single intravitreal (IVT) dose in rabbits and monkeys. Rabbits (2.5 mg/eye; n = 40) and monkeys (2.5 mg/eye; n = 12) received TMAB001 as a bilateral IVT dose. TMAB001 concentrations were measured in ocular tissues in all rabbits and monkeys by enzyme-linked immunosorbent assay (ELISA)...
April 27, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29708866/the-characterization-of-monoclonal-antibodies-to-mouse-tlt-1-suggests-that-tlt-1-plays-a-role-in-wound-healing
#18
Barbara Manfredi, Jessica Morales-Ortíz, Lymarie M Díaz-Díaz, Liz Hernandez-Matias, Delmaliz Barreto-Vázquez, Javier Menéndez-Pérez, J Alejandro Rodríguez-Cordero, Juan C Villalobos-Santos, Edgardo Santiago-Rivera, Adriana Rivera-Dompenciel, Eunice L Lozada-Delgado, Madhavi Kuchibhotla, Kelvin Carrasquillo-Carrión, Abiel Roche-Lima, A Valance Washington
Platelets play a vital role in hemostasis and inflammation. The membrane receptor TREM-like transcript-1 (TLT-1) is involved in platelet aggregation, bleeding, and inflammation, and it is localized in the α-granules of platelets. Upon platelet activation, TLT-1 is released from α-granules both in its transmembrane form and as a soluble fragment (sTLT-1). Higher levels of sTLT-1 have been detected in the plasma of patients with acute inflammation or sepsis, suggesting an important role for TLT-1 during inflammation...
April 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29669835/characterization-of-three-novel-linear-neutralizing-b-cell-epitopes-in-the-capsid-protein-of-swine-hepatitis-e-virus
#19
Yiyang Chen, Baoyuan Liu, Yani Sun, Huixia Li, Taofeng Du, Yuchen Nan, Julian A Hiscox, En-Min Zhou, Qin Zhao
Hepatitis E virus (HEV) causes liver disease in humans and is thought to be a zoonotic infection with domestic animals being a reservoir including swine and rabbits. One of the proteins encoded by the virus is the capsid protein. This is likely the major immune-dominant protein and a target for vaccination. Four monoclonal antibodies (MAbs); three novel; 1E4, 2C7, 2G9, and one previously characterized (1B5), were evaluated for binding to the capsid protein from genotype 4 (swine) hepatitis E virus (HEV). The results indicated that 625 DFCP628 , 458 PSRPF462 , and 407 EPTV410 peptides on the capsid protein comprised minimal amino acid sequence motifs recognized by 1E4, 2C7, and 2G9, respectively...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29627856/generation-and-characterization-of-a-novel-recombinant-scfv-antibody-specific-for-campylobacter-jejuni
#20
Ruramayi M Nzuma, Fuquan Liu, Irene R Grant
Campylobacter jejuni is a leading cause of foodborne illness worldwide, mainly due to consumption and handling of contaminated raw chicken. Rapid detection methods for C. jejuni are vital for monitoring contamination levels in chicken products and reducing human Campylobacteriosis cases. The 'gold standard' culture-based method of Campylobacter detection takes 3-5 days and is too slow to permit effective intervention. Immuno-based methods are faster, but usually necessitate use of animals or hybridoma technology to produce antibodies; making them difficult and expensive to produce...
June 2018: Applied Microbiology and Biotechnology
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